Culture dish rack fixing bracket, incubator and biological sample morphology imaging equipment

Abstract

The disclosed embodiment discloses a culture dish rack fixing bracket, an incubator, and a biological sample morphology imaging device, wherein the culture dish rack fixing bracket comprises: a bracket body and a rack fixing member; the bracket body comprises a plurality of first brackets arranged at intervals, and at least one rack fixing member is fixed on each of the first brackets; the rack fixing member comprises two rows of mounting positions, and the adjacent mounting positions on the rack fixing members of two adjacent first brackets are used to fix a culture dish rack. This technical solution can fix multiple culture dish racks on a plurality of adjacent mounting positions through a universal rack fixing member, and then use an imaging device to perform imaging analysis on the biological samples in the culture dish racks in sequence, thereby improving the sample analysis throughput.

Description

Technical Field

[0001] The present disclosure relates to the technical field of biological sample detection, and in particular to a culture dish rack fixing bracket, a culture box and a biological sample morphology imaging device. Background Art

[0002] In traditional biological research, due to the limitations of experimental methods, we can usually only study the static state of organisms at a certain time section, reflecting the cumulative effect of all biological dynamic processes before that time point. However, the growth and development of organisms is a combination of a series of time-space specific processes. Only by observing the dynamic change process can we more comprehensively understand the spatiotemporal specificity of biological growth and development regulation.

[0003] The prior art provides a device that needs to be placed in a dark greenhouse with a constant temperature to maintain the stability of the biological growth environment, and controls the movement of the camera to switch the camera field of view between samples to observe the growth and development of the organism under different temporal and spatial characteristics. However, the device cannot achieve simultaneous analysis of large-scale samples, and can only provide a single experimental environment, which cannot meet the requirements of applying a normal culture light source and controlling the most basic conditions for biological growth such as temperature, oxygen, and carbon dioxide. Summary of the invention

[0004] In order to solve the problems in the related art, the embodiments of the present disclosure provide a culture dish rack fixing bracket, a culture box and a biological sample morphology imaging device.

[0005] In a first aspect, an embodiment of the present disclosure provides a culture dish rack fixing bracket.

[0006] Specifically, the culture dish rack fixing bracket includes: a bracket body and a rack fixing piece; the bracket body includes a plurality of first brackets arranged at intervals, and at least one rack fixing piece is fixed on each of the first brackets; the rack fixing piece includes two rows of mounting positions, and the adjacent mounting positions on the rack fixing pieces of two adjacent first brackets are used to fix a culture dish rack.

[0007] Optionally, the rack fixing component includes: a bracket fixing part, a first rack part and a second rack part arranged in a pair; the first rack part and the second rack part are respectively located on both sides of the bracket fixing part; wherein the first rack part and the second rack part are each provided with at least two mounting holes; the mounting holes form a row of mounting positions.

[0008] Optionally, the first mounting portion and the second mounting portion are respectively arranged at an obtuse angle to the support fixing portion.

[0009] Optionally, the mounting hole is a through hole structure or a U-shaped groove.

[0010] Optionally, the rack fixing members are fixed at different height positions of the same first bracket; and / or

[0011] The rack fixing parts are fixed at the same height positions of a plurality of the first brackets.

[0012] Optionally, the heights of the mounting holes on two rack fixing members for fixing the same culture dish rack match, and the heights of the two rack fixing members are the same or different.

[0013] Optionally, a row of mounting positions of the rack fixture is composed of two mounting holes, which are used to mount a small culture dish rack on one side of the rack fixture; or

[0014] A row of mounting positions of the rack fixture is composed of more than four mounting holes, which are used to mount more than two small culture dish racks, or at least one large culture dish rack, on the left or right side of the rack fixture; or

[0015] The lengths of the mounting fixtures of two adjacent first brackets are different, and the longer mounting fixture matches the height of the mounting hole on at least one shorter mounting fixture.

[0016] Optionally, the first bracket is also provided with an electrical socket interface for achieving electrical connection with the culture dish rack.

[0017] Optionally, the electrical socket interface includes an electrical circuit connector having control pins.

[0018] Optionally, the control pins in the electrical circuit connector include:

[0019] Light source common cathode / common anode pins and / or

[0020] Top light source pin 1 and / or

[0021] Top mounted second light source pin and / or

[0022] Top third light source pin and / or

[0023] Top mounted 4th light source pin and / or

[0024] Side light pins and / or

[0025] Servo motor power pins and / or

[0026] Servo motor ground pin and / or

[0027] Servo motor data bus pins.

[0028] Optionally, the first bracket is also provided with an air pump fixing plate, an air pump shock absorbing sleeve and an air pump; a plurality of the air pump shock absorbing sleeves are fixed on the air pump fixing plate, and the air pump is arranged inside the air pump shock absorbing sleeve; the air pump is used for gas exchange inside and outside the culture dish rack.

[0029] Optionally, a pneumatic joint is further provided on the first bracket, which is connected to the gas pipeline of the air pump and is used to transport gas into the culture dish rack.

[0030] Optionally, a liquid delivery connector is further provided on the first bracket for delivering liquid into the culture dish rack.

[0031] Optionally, the culture dish rack fixing bracket further comprises:

[0032] A rotating platform, the bracket body is fixedly connected to the rotating platform;

[0033] Wherein, the support body further includes a second support, the second support is radially distributed, and the end of the second support extending out of the rotating platform is fixed with the first support.

[0034] Optionally, the rotating platform includes: a base, a flange fixed to the base, a baffle, and a photoelectric limit switch fixed to the base;

[0035] The baffle is fixed at the gap of the flange, and the height of the baffle matches the height of the photoelectric limit switch; the baffle is provided with a notch, and the optical signal of the photoelectric limit switch can pass through the notch.

[0036] Optionally, the first bracket is further provided with an electrical socket interface, and the culture dish rack fixing bracket further comprises:

[0037] A branching plate, fixed above the rotating platform;

[0038] Wherein, a conductive slip ring is arranged inside the rotating platform, and the cables connected to the electrical socket interface are electrically connected to the conductive slip ring after being joined via the branching board.

[0039] Optionally, a mounting hole is provided on the top of the rotating platform, and the second bracket and the branching plate are fixed through the mounting hole; the second bracket is fixed between the branching plate and the rotating platform;

[0040] The rotating platform is also provided with a central through hole, and the cables connected to the electrical socket interface are electrically connected to the conductive slip ring through the central through hole after being joined via the branching board.

[0041] Optionally, the culture dish rack fixing bracket further includes: an electrical equipment cover fixed above the second bracket; and an air pump pipe outlet is provided on the electronic equipment cover.

[0042] Optionally, a liquid delivery connector is further provided on the first bracket and is connected to the liquid pipeline of the conductive slip ring.

[0043] Optionally, the liquid delivery connector is arranged on a side of the electrical socket interface.

[0044] Optionally, the mounting holes include: a bracket mounting hole and a branch board mounting hole; the bracket mounting holes and the branch board mounting holes are arranged at intervals.

[0045] In a second aspect, an embodiment of the present disclosure provides an incubator.

[0046] Specifically, the interior of the incubator is provided with a culture dish rack fixing bracket as described in any one of the first aspects.

[0047] In a third aspect, an embodiment of the present disclosure provides a biological sample morphology imaging device.

[0048] Specifically, the biological sample morphology imaging device comprises: an incubator and an imaging device as described in the second aspect; the imaging device is used to photograph the morphology of the biological samples inside the incubator.

[0049] The technical solution provided by the embodiments of the present disclosure may have the following beneficial effects:

[0050] The culture dish rack fixing bracket provided in the embodiment of the present disclosure can fix multiple culture dish racks on several adjacent installation positions through a universal rack fixing piece, and then use an imaging device (such as a CCD camera or a CMOS camera) to perform imaging analysis on the biological samples in the culture dish racks in turn, thereby improving the sample analysis throughput.

[0051] It is to be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present disclosure. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Other features, objectives and advantages of the present disclosure will become more apparent through the following detailed description of non-limiting embodiments in conjunction with the accompanying drawings. In the accompanying drawings:

[0053] Figure 1 A front view of a culture dish rack fixing bracket according to an embodiment of the present disclosure is shown;

[0054] Figure 2 A schematic diagram showing the structure of a rack fixing member according to an embodiment of the present disclosure is shown;

[0055] Figure 3A schematic diagram showing the structure of the electrical connection between the conductive slip ring and the electrical socket interface according to an embodiment of the present disclosure;

[0056] Figure 4 A schematic diagram showing a culture dish rack fixing bracket fixing a culture dish rack according to an embodiment of the present disclosure;

[0057] Figure 5 Show Figure 1 Schematic diagram of electrical equipment cover removed;

[0058] Figure 6 A top view of a culture dish rack fixing bracket according to an embodiment of the present disclosure is shown;

[0059] Figure 7 A schematic diagram showing the position relationship of a photoelectric limit switch according to an embodiment of the present disclosure;

[0060] Figure 8 A schematic diagram showing the structure of an incubator according to an embodiment of the present disclosure is shown;

[0061] Fig. 9 A schematic structural diagram of a biological sample morphology imaging device according to an embodiment of the present disclosure is shown. DETAILED DESCRIPTION

[0062] Hereinafter, exemplary embodiments of the present disclosure will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily implement them. In addition, for the sake of clarity, parts not related to the description of the exemplary embodiments are omitted in the accompanying drawings.

[0063] In the present disclosure, it should be understood that terms such as "include" or "have" are intended to indicate the presence of features, numbers, steps, behaviors, components, parts, or a combination thereof disclosed in the present specification, and are not intended to exclude the possibility that one or more other features, numbers, steps, behaviors, components, parts, or a combination thereof exist or are added.

[0064] It should also be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present disclosure may be combined with each other. The present disclosure will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0065] The prior art provides a device that needs to be placed in a dark greenhouse with a constant temperature to maintain a stable plant growth environment, and controls the movement of the camera to switch the camera field of view between samples to observe the growth and development of organisms under different temporal and spatial characteristics. However, the device cannot achieve simultaneous analysis of large-scale samples, and can only provide a single experimental environment, which cannot meet the requirements of applying a normal culture light source and controlling the most basic conditions for biological growth such as temperature, oxygen, and carbon dioxide.

[0066] In basic scientific research in botany, including but not limited to plant resistance to environmental stress (resistance to high temperature, frost, salt, hypoxia, and oxidative damage), light signal pathways, photosynthesis, plant hormone signal pathways, plant developmental biology, plant breeding, etc., it is necessary to study dynamic temporal and spatial specificity, and therefore requires matching experimental techniques.

[0067] The present disclosure is made to at least partially solve the problems in the prior art discovered by the inventors.

[0068] like Figure 1-Figure 7 As shown, an embodiment of the present disclosure provides a culture dish rack fixing bracket, the culture dish rack fixing bracket 1 comprises: a bracket body 11 and a rack fixing member 12; the bracket body 11 comprises a plurality of first brackets 111 arranged at intervals, and at least one rack fixing member 12 is fixed on each of the first brackets 111; the rack fixing member 12 comprises two rows of mounting positions, and the adjacent mounting positions on the rack fixing members 12 of two adjacent first brackets 111 are used to fix a culture dish rack.

[0069] The culture dish rack fixing bracket provided in the embodiment of the present disclosure can fix multiple culture dish racks on several adjacent installation positions through a universal rack fixing piece, and then use an imaging device (such as a CCD camera or a CMOS camera) to perform imaging analysis on the biological samples in the culture dish racks in turn, thereby improving the sample analysis throughput.

[0070] According to an embodiment of the present disclosure, the mounting fixture 12 includes: a bracket fixing portion 121, a first mounting portion 122 and a second mounting portion 123 arranged in pairs; the first mounting portion 122 and the second mounting portion 123 are respectively located on both sides of the bracket fixing portion 121; wherein the first mounting portion 122 and the second mounting portion 123 are both provided with at least two mounting holes; the mounting holes form a row of mounting positions.

[0071] In the embodiment of the present disclosure, the first mounting portion 122 and the second mounting portion 123 are respectively arranged at an obtuse angle to the support fixing portion 121, thereby presenting an open and closed state, which facilitates the operation of fixing the culture dish support.

[0072] In the embodiment of the present disclosure, the mounting holes include an upper mounting hole A1 and a lower mounting hole A2; the upper mounting hole A1 and the lower mounting hole A2 form a row of mounting positions.

[0073] In the embodiment of the present disclosure, the upper mounting hole A1 and the lower mounting hole A2 can be through holes, or can be U-shaped grooves or L-shaped grooves. The culture dish bracket can be fixed by screws, nuts and other parts passing through the through holes or U-shaped grooves, or pushed into the horizontal part of the L-shaped groove and then sliding downward into the vertical part. The present disclosure does not impose any restrictions on this.

[0074] In the embodiment of the present disclosure, a row of mounting positions is not limited to two mounting holes, and a row of mounting positions may also be composed of multiple mounting holes. Suitable mounting holes on the mounting positions are selected for fixing according to the size of the culture dish fixing rack, and the present disclosure does not impose any restrictions on this.

[0075] According to an embodiment of the present disclosure, the rack fixing member 12 is fixed at different height positions of the same first bracket 111 ; and / or the rack fixing member 12 is fixed at the same height position of several first brackets 111 .

[0076] In the manner of the embodiment of the present disclosure, a plurality of rack fixings 12 can be set on each first bracket 111, and the heights of the rack fixings 12 set on different first brackets 111 are the same, so that the installation positions on different first brackets 111 are close in height, which facilitates the use of the installation positions to fix multiple culture dish racks in layers on the bracket body 11, thereby increasing the number of fixed culture dish racks.

[0077] It is understandable that if more culture dish racks need to be fixed, it is only necessary to increase the height of the first bracket 111 and the number of rack fixing members 12 accordingly, and to set more layers of mounting positions, which will not be elaborated in the present disclosure.

[0078] According to an embodiment of the present disclosure, the heights of the mounting holes on the two rack fixing members 12 for fixing the same culture dish rack match, and the heights of the two rack fixing members 12 are the same or different.

[0079] In the embodiment of the present disclosure, the two rack fixing members 12 fixing the same culture dish rack can be universal members. When the heights of the two rack fixing members are the same, the mounting holes on the mounting positions are in an aligned state, which facilitates the fixing of the culture dish rack.

[0080] In the embodiment of the present disclosure, the position fixing members 12 on the first brackets 111 may be staggered by one or several mounting holes, and the positions of other mounting holes must be aligned. Similarly, multiple culture dish brackets may be fixed in layers using the mounting positions, and the present disclosure does not impose any restrictions on this.

[0081] According to an embodiment of the present disclosure, a row of mounting positions of the rack fixture 12 is composed of two mounting holes, which are used to mount a small culture dish rack on one side of the rack fixture 12; or

[0082] A row of mounting positions of the rack fixture 12 is composed of more than four mounting holes, which are used to mount more than two small culture dish racks, or at least one large culture dish rack, on the left or right side of the rack fixture 12; or

[0083] The lengths of the mounting fixtures 12 of two adjacent first brackets 111 are different, and the longer mounting fixture 12 matches the height of the mounting hole on at least one shorter mounting fixture 12 .

[0084] In the manner of the embodiment of the present disclosure, for example, two mounting holes can be opened on the rack fixture 12, or four mounting holes can be opened as a row of mounting positions. Fixing a small culture dish rack usually requires two mounting holes to ensure stability. Therefore, a small culture dish rack can be fixed using a rack fixture 12 with two mounting holes, or two small culture dish racks can be fixed using a rack fixture 12 with four mounting holes. Of course, a large culture dish rack can also be fixed using a rack fixture 12 with four mounting holes to ensure stability. By analogy, culture dish racks of different sizes and quantities can be installed according to the number of mounting holes opened on the rack fixture 12, and the present disclosure does not limit this.

[0085] In the embodiment of the present disclosure, the rack fixing member 12 can be a non-universal member, that is, the lengths of the rack fixing members 12 of two adjacent first brackets 111 can be different. When fixing the culture dish rack, the longer rack fixing member 12 matches the height of the mounting hole on at least one shorter rack fixing member 12. For example, after the mounting holes are aligned or the positions of one or several mounting holes are misaligned, the other mounting holes are aligned, and the culture dish rack can be fixed.

[0086] According to an embodiment of the present disclosure, the first bracket 111 is also provided with an electrical socket interface 13 for electrically connecting to the culture dish rack. The electrical socket interface 13 can be fixed on each first bracket 111, and the number can be flexibly set as required, which is not limited by the present disclosure.

[0087] According to an embodiment of the present disclosure, the electrical socket interface 13 includes: an electrical circuit connector having a control pin.

[0088] In the embodiment of the present disclosure, the electrical circuit connector may include: a 9-pin wiring socket, which can control 5 light sources and multiple servo motors simultaneously.

[0089] The specific pin definitions are as follows:

[0090] 1- common cathode / common anode of light source, 2- top-mounted 1st light source, 3- top-mounted 2nd light source, 4- top-mounted 3rd light source, 5- top-mounted 4th light source, 6- side-mounted light source, 7- servo motor power supply (VCC pin), 8- servo motor ground (GND pin), 9- servo motor data bus (DATA pin).

[0091] Light sources with different wavelengths and / or light intensities can be provided in the culture dish rack as culture light sources for biological samples. The electrical socket interface 13 is used to electrically connect to the light source in the culture dish rack, thereby providing light source conditions for the culture of biological samples. For example, an overhead culture light source and a side culture light source can be provided on the culture dish rack. Among them, the overhead culture light source is located at the top of the culture dish rack, and the side culture light source is located at the side of the culture dish rack. For the overhead culture light source, a 9-pin plug is used but the cable is only connected to pins 1 to 5. For the side culture light source, the cable is only connected to pins 1 and 6. The culture dish rack can also fix a gravity module, which can change the gravity direction of the biological sample in the culture dish. For the gravity module, the cable is only connected to pins 7 to 9. All modules use 9-pin plugs of the same specification and can be inserted into any electrical socket interface on the first bracket 111, without distinguishing the position and function of each interface.

[0092] The servo motor is controlled by a data bus. Multiple motors are connected to the same data bus. According to the address information contained in the data packet, the servo motor with the corresponding address number on the bus responds and executes the instruction.

[0093] The electrical circuit connector can adopt a flat cable socket or other types of connectors such as an aviation plug to achieve quick connection and disconnection of cables. The number of pins of the connector is not limited to 9 pins. It can be determined whether the multiple LED light sources use a common cathode / common anode or a set of independent cathode / anode pins for each light source based on the rated current of each pin of the connector. The pins used by the servo motor can also adopt a single-pin data pin (TTL signal control mode) or a 2-pin data pin (RS485 signal control mode) according to the type of data bus. Other pins can also be reserved for subsequent function expansion, and the present disclosure does not limit this.

[0094] According to an embodiment of the present disclosure, the culture dish rack fixing bracket 1 also includes: a rotating platform 14; the bracket body 11 is fixedly connected to the rotating platform 14, and the bracket body 11 also includes a second bracket 112, the second bracket 112 is radially distributed, and the end of the second bracket 112 extending out of the rotating platform 14 is fixed with the first bracket 111.

[0095] The culture dish rack fixing bracket provided in the embodiment of the present disclosure can, driven by a rotating platform, allow different culture dish racks to be respectively aligned with imaging devices (such as CCD cameras or CMOS cameras) set at fixed positions, thereby conveniently and simultaneously performing imaging analysis on biological samples in multiple culture dish racks, thereby improving the efficiency of sample analysis.

[0096] According to an embodiment of the present disclosure, the rotating platform 14 includes: a base 141, a flange 142 fixed to the base 141, a baffle 143 and a photoelectric limit switch 144 fixed to the base 141;

[0097] The baffle 143 is fixed at the gap of the flange 142 , and the height of the baffle 143 matches the height of the photoelectric limit switch 144 ; the baffle 143 has a notch 1431 , and the optical signal of the photoelectric limit switch 144 can pass through the notch 1431 .

[0098] In the method of the embodiment of the present disclosure, the photoelectric limit switch 144 is used to calibrate the zero point position of the rotating platform 14. When the rotating platform 14 rotates, the baffle 143 can block the light signal of the photoelectric limit switch 144, and the light signal at the notch 1431 can pass through. The zero point position can be calibrated by detecting the light signal so that the culture dish rack can be aligned with the imaging device.

[0099] An exemplary calibration method is as follows:

[0100] a) Rotate counterclockwise at a speed of about 25° / s until the photoelectric limit switch signal is turned on and stops moving;

[0101] b) Rotate clockwise at a speed of about 10° / s until the photoelectric limit switch signal is disconnected and stops moving;

[0102] c) Rotate counterclockwise at a speed of about 0.1° / s until the photoelectric limit switch signal is turned on and stops moving;

[0103] d) Rotate 1° clockwise at normal drive speed and set the current position as the zero point.

[0104] The zero point position is calibrated by the above calibration method to achieve accurate positioning of the biological sample in the culture dish rack by the imaging device, which can improve the accuracy of biological sample analysis.

[0105] Those skilled in the art will appreciate that other clockwise or counterclockwise rotation methods and rotation speeds may be used to calibrate the zero point position, and the present disclosure does not limit this.

[0106] According to an embodiment of the present disclosure, the culture dish rack fixing bracket further includes: a corner reinforcement block 113 , which is disposed between adjacent second brackets 112 and is used to reinforce the bracket body 11 .

[0107] In the embodiment of the present disclosure, the corner reinforcement block 113 is disposed at a position of the first bracket 111 close to the second bracket 112 to ensure the stability of the bracket body 20 during the rotation process.

[0108] According to an embodiment of the present disclosure, an electrical socket interface 13 is also provided on the first bracket 111, and the culture dish rack fixing bracket also includes: a branching plate 15, which is fixed above the rotating platform 14, wherein a conductive slip ring 145 is provided inside the rotating platform 14, and the cables connected to the electrical socket interface 13 are electrically connected to the conductive slip ring 145 after being converged via the branching plate 15.

[0109] In the embodiment of the present disclosure, the number of electrical socket interfaces 13 can correspond to the number of culture dish racks, which is convenient for connecting the electrical socket interfaces 13 and the culture dish racks nearby. The cables led out from different first brackets 111 are converged through the branching plate 15 and electrically connected to the conductive slip ring 145 inside the rotating platform 14, ensuring that the cables can rotate along with the culture dish racks when the first bracket 111 rotates and is driven by the culture dish racks, without cable entanglement, and the electrical signals can be reliably transmitted from the conductive slip ring 145 to the electrical socket interfaces 13.

[0110] Take the 8-way branch circuit board as an example. Figure 3 As shown, the electrical socket interfaces 1-16 are respectively electrical socket interfaces fixed on a plurality of first brackets 111, and the branching circuit board electrically connects the eight cables introduced by the conductive slip ring to each electrical socket interface respectively, wherein each electrical socket interface is electrically connected to the eight cables branched out through the eight-way branching circuit board.

[0111] According to an embodiment of the present disclosure, a mounting hole 146 is provided on the top of the rotating platform 14, and the second bracket 112 and the wiring board 15 are fixed through the mounting hole 146. The second bracket 112 is fixed between the wiring board 15 and the rotating platform 14. The rotating platform 14 is also provided with a central through hole 147, and the cables connected to the electrical socket port 13 are electrically connected to the conductive slip ring 145 through the central through hole 147 after being merged through the wiring board 15.

[0112] According to an embodiment of the present disclosure, the mounting holes 146 include: a bracket mounting hole 1461 and a branching board mounting hole 1462; the bracket mounting holes 1461 and the branching board mounting holes 1462 are arranged at intervals. The second bracket 112 is fixed through the bracket mounting holes 1461 to form a radial distribution, and the branching board 15 is fixed above the rotating platform 14 through the branching board mounting holes 1462. The second bracket 112 extends from the gap between the vertical mounting parts that fix the branching board 15 to the outside of the table of the rotating platform 14 to form a free end, and the first bracket 111 is installed on the free end.

[0113] According to an embodiment of the present disclosure, there are at least two groups of the bracket mounting holes 1461 , thereby forming at least one group of adjacent first brackets 111 , so as to facilitate fixing the culture dish rack in the space portion of the adjacent first brackets 111 .

[0114] According to an embodiment of the present disclosure, the bracket mounting holes 1461 are 8 groups, and the angle between each group is 45°, thereby forming an eight-way main bracket, that is, the number of first brackets 111 is 8, and there are 8 groups of adjacent first brackets 111 for fixing the culture dish rack.

[0115] Those skilled in the art will appreciate that the bracket mounting holes 1461 may be in other groups, and the angles between each group may be in other angles, so as to form a main bracket in other directions besides eight directions. The present disclosure does not limit this.

[0116] In the method of the embodiment of the present disclosure, each group of bracket mounting holes 1461 can be used to fix a group of second brackets 112, so there are 8 groups of first brackets 111 and second brackets 112. After the culture dish racks are fixed on adjacent first brackets 111, for the imaging device at a fixed position, it can be rotated in 8 directions so that the culture dish racks in each direction are aligned with the imaging device, and then the imaging analysis of the biological sample is performed. It should be noted that multiple culture dish racks can be set at different heights on the first bracket 111. When performing imaging analysis, it is only necessary to adjust the height of the imaging device to image the biological sample in the culture dish rack without adjusting the alignment position of the culture dish rack.

[0117] In the embodiment of the present disclosure, the number of each group of bracket mounting holes 1461 can be 1-5, providing support for fixing the bracket body 11. The specific number can be flexibly adjusted as needed, and the present disclosure does not impose any restrictions on this.

[0118] According to an embodiment of the present disclosure, the conductive slip ring 145 includes: a stator part and a rotor part; the rotor part is fixed to the table top of the rotating platform 14; and the stator part is fixed to the base 141 of the rotating platform 14.

[0119] According to an embodiment of the present disclosure, the culture dish rack fixing bracket also includes: an air pump fixing plate 16, an air pump shock absorbing sleeve 17 and an air pump 18; the air pump fixing plate 16 is fixed above the branching plate 15, and a plurality of the air pump shock absorbing sleeves 17 are fixed on the air pump fixing plate 16, and the air pump 18 is arranged inside the air pump shock absorbing sleeve 17; the air pump 18 is used for gas exchange inside and outside the culture dish rack.

[0120] In the embodiment of the present disclosure, air, oxygen, carbon dioxide, ethylene and other gases can be pumped into the culture dish rack through an air pump to ensure that the air composition of the airtight culture dish is consistent with that in the incubator, which is used to study the growth status of plants under the experimental conditions, thereby meeting the needs of different experimental conditions.

[0121] According to an embodiment of the present disclosure, the culture dish rack fixing bracket also includes: an electrical equipment cover 19, fixed above the second bracket 112; an air pump pipe outlet 191 is provided on the electrical equipment cover 19, and the air pump pipe connected to the air pump 18 is connected to the culture dish rack through the air pump pipe outlet 191.

[0122] According to an embodiment of the present disclosure, the culture dish rack fixing bracket further includes: a pneumatic joint (not shown in the figure), which is connected to the gas pipeline of the air pump 19 and is arranged on the first bracket 111.

[0123] In the embodiment of the present disclosure, the pneumatic connector is arranged on the side of the electrical socket interface 13. For example, a connector (quick-plug type, quick-twist type or pagoda type connector) for connecting a pneumatic hose can be installed, which is connected to the air pump 19 through a hose buried in the bracket body 11, and connected to the air inlet of the culture dish rack through the hose.

[0124] According to an embodiment of the present disclosure, the culture dish rack fixing bracket further includes: a liquid delivery connector (not shown in the figure), which is connected to the liquid pipeline of the conductive slip ring 145 and is arranged on the first bracket 111 for providing culture liquid to the culture dish rack.

[0125] In the embodiment of the present disclosure, the liquid delivery connector is arranged on the side of the electrical socket interface 13 to provide culture fluid for the biological samples in each culture dish rack. If the liquid pipeline is connected, the conductive slip ring 145 should be an electric-liquid hybrid slip ring, and other liquid pipeline components such as the culture fluid storage bottle and the peristaltic pump can be arranged on the air pump fixing plate 16, connected with a hose, and introduced into the bracket body 11 until the liquid delivery connector or through the conductive slip ring 145.

[0126] Those skilled in the art will appreciate that the culture medium storage bottle, peristaltic pump, and liquid input connector may also be used to transport other liquids besides culture medium to the culture dish rack, and the present disclosure does not limit this.

[0127] The culture dish rack fixing bracket provided in the embodiment of the present disclosure can be fixed to the optical seismic isolation platform to prevent external vibration from affecting the alignment accuracy of the culture dish rack and the imaging device. When using the imaging device to perform imaging analysis on the biological samples in the culture dish rack, it is necessary to calibrate the zero point position of the rotating platform. After calibration, the rotation speed of the rotating platform can be controlled by a stepper motor or a direct drive motor. The rotating platform drives the bracket body to rotate so that the culture dish rack that needs to be imaged in each direction can be aligned with the imaging device at a fixed position, and then the imaging device is used to analyze the sample photos taken. The culture dish rack fixing bracket provided in the embodiment of the present disclosure can fix multiple culture dish racks, so that imaging analysis can be performed on biological samples in multiple culture dish racks at the same time, which improves the sample analysis throughput.

[0128] like Figure 8 As shown, the present disclosure provides a culture box, the culture box 2 can be fixed to the optical seismic isolation platform, including: a culture dish rack fixing bracket 1 and a box body 20. The culture dish rack fixing bracket is shown in FIG. Figure 1-Figure 7 The embodiments shown are not described in detail here.

[0129] The bottom of the box 20 is provided with an opening 21, and the culture dish rack fixing bracket is fixed to the optical seismic isolation platform through the opening 21 to avoid affecting the stability of the rotating platform 11 during operation due to insufficient mechanical strength of the box 20, and to avoid direct transmission of the vibration of the box 20 itself to the culture dish rack fixing bracket. A thin rubber pad can be added between the opening 21 and the optical seismic isolation platform to avoid damaging the air tightness of the box 20 here. An observation window 3 is installed on one of the side walls of the box 20, and the culture dish rack fixing bracket is rotated, and each culture dish rack A can be observed through the observation window 3.

[0130] According to an embodiment of the present disclosure, an aviation socket panel 23 is installed on the box 20 to provide an electrical connection interface for the culture dish rack fixing bracket. The electrical connection between the internal equipment of the box 20 and the outside world is through the aviation socket on the panel to ensure that the wiring is light-proof and air-tight.

[0131] According to an embodiment of the present disclosure, the incubator 2 further includes: a temperature control device for providing a constant temperature environment for the incubator; the temperature control device includes: a compressor 24, which is arranged outside the incubator and connected to the box body 20 by a copper tube, so as to avoid the vibration generated by the compressor when working from being transmitted to the culture dish rack fixing bracket, thereby further improving the alignment accuracy of the biological sample morphology imaging device and the culture dish rack.

[0132] The temperature control device also includes: a condenser, an evaporator, a circulating fan, a temperature sensor, a control circuit and other parts. Except for the compressor 24, the other parts of the temperature control device are arranged in the box body 20, and a bottom long strip air outlet 25 is also provided in the box body 20. When the door of the box body 20 is closed, the air cooled or heated by the temperature control device is blown out from the bottom long strip air outlet 25 and sucked back from the top circulating fan to maintain a constant temperature environment in the enclosed space in the box body 20.

[0133] According to an embodiment of the present disclosure, an air inlet 26 and an exhaust port 27 are provided on the box body 20, as well as a solenoid valve located at the exhaust port 27; the air inlet 26 is used to connect to an air supply component, and the air supply component is used to provide a gas environment in the incubator; the solenoid valve is used to control the opening and closing state of the exhaust port 27.

[0134] In the embodiment of the present disclosure, the gas supply assembly connected to the air inlet 26 can provide an experimental environment such as air, oxygen, carbon dioxide, ethylene, etc. for the enclosed space in the box 20, and the air pump 19 can pump the gas in the box 20 into the corresponding culture dish rack to study the growth status of biological samples under the experimental environment.

[0135] It should be noted that in order to avoid the influence of the original gas in the box 20 on the experimental conditions (such as gas concentration), the exhaust port 27 is opened by the solenoid valve, and high-pressure air is introduced to quickly flush the gas in the box 20 and discharge it outside the box 20, and then the exhaust port 27 is closed by the solenoid valve, and the gas that meets the experimental conditions can be introduced into the box 20 through the air supply assembly. After closing the door, the inside of the box 20 remains strictly sealed, except for the observation window, it is opaque and airtight. When the solenoid valve at the exhaust port 27 is closed, the gas introduced from the air inlet 26 can generate a small positive pressure inside the box, which can prevent the external air from infiltrating from the possible gaps caused by the production and manufacturing process problems and polluting the internal environment.

[0136] According to the embodiments of the present disclosure, the imaging device on the outside of the box 20 can photograph the biological sample through the observation window 22. The glass used for the observation window 22 is double-sided coated, and the transmittance of the glass in the visible light and infrared light bands is increased at the same time, so as to reduce the reflection of the infrared lighting source on the outside of the box 20 into the lens, and reduce the loss of visible light signals of the biological samples in the box 20 when detected by the sensor on the outside of the box 20.

[0137] According to an embodiment of the present disclosure, heating elements are provided around the observation window 22 to prevent frost on the surface of the observation window glass when the internal low temperature environment is low.

[0138] like Fig. 9As shown, the present disclosure provides a biological sample morphology imaging device, which includes: a culture dish rack fixing support 1, a culture box 2, an optical seismic isolation platform 3 and an imaging device 4; the culture box is shown in FIG. Figure 8 The incubator 2 and the imaging device 4 are both fixed to the optical vibration isolation platform 3 .

[0139] The imaging device 4 is used to photograph the morphology of the biological sample inside the culture dish rack through the observation window.

[0140] According to the embodiments of the present disclosure, with the plane where the observation window is located as the Z-axis direction in the three-dimensional space, the shooting component of the imaging device can move in the X-axis, Y-axis, and Z-axis directions in the three-dimensional space. By adjusting the displacement of the imaging device in the X-axis, Y-axis, and Z-axis directions, and rotating the corresponding culture dish rack A to align with the shooting component through the rotating platform, it is possible to image the biological samples in several culture dish racks A fixed on the culture dish rack fixing bracket, and then perform imaging analysis without adjusting the alignment position of the culture dish rack. It is also possible to image multiple plant samples in the same culture dish rack A, and autofocus can be achieved, thereby achieving high-throughput imaging.

[0141] The biological sample morphology imaging device provided by the embodiment of the present disclosure can be used for analyzing the growth status of plant samples, especially plant seedlings. By rotating the culture dish rack fixing bracket, a large number of plant seedling samples can be photographed simultaneously, thereby improving the analysis throughput. Moreover, while meeting the requirements of high-throughput imaging analysis, the temperature, gas component concentration, light and other conditions of the plant seedling culture environment in the culture dish rack can be flexibly adjusted, thereby meeting the requirements of observing the changes in the growth of plant seedlings under a variety of experimental condition variables, thereby greatly expanding the function of the device.

[0142] The above description is only a preferred embodiment of the present disclosure and an explanation of the technical principles used. Those skilled in the art should understand that the scope of the invention involved in the present disclosure is not limited to the technical solution formed by a specific combination of the above technical features, but should also cover other technical solutions formed by any combination of the above technical features or their equivalent features without departing from the inventive concept. For example, the above features are replaced with the technical features with similar functions disclosed in the present disclosure (but not limited to) by each other.

Claims

1. A culture dish rack fixing bracket, characterized in that: include: A support body and a rack fixing member; the support body comprises a plurality of first supports arranged at intervals, and at least one rack fixing member is fixed on each of the first supports; the rack fixing member comprises two rows of mounting positions, and the adjacent mounting positions on the rack fixing members of two adjacent first supports are used to fix a culture dish rack; It also includes: a rotating platform, the bracket body is fixedly connected to the rotating platform; wherein the bracket body also includes a second bracket, the second bracket is radially distributed, and the first bracket is fixed to the end of the second bracket extending out of the rotating platform; The first bracket is provided with an electrical socket interface, a pneumatic joint and a liquid delivery joint; the electrical socket interface is used to achieve electrical connection with the culture dish rack, the pneumatic joint is used to deliver gas into the culture dish rack, and the liquid delivery joint is used to deliver liquid into the culture dish rack.

2. The culture dish rack fixing bracket according to claim 1, characterized in that: The rack fixing member includes: a bracket fixing part, a first rack part and a second rack part arranged in pairs; the first rack part and the second rack part are respectively located on both sides of the bracket fixing part; wherein the first rack part and the second rack part are each provided with at least two mounting holes; the mounting holes form a row of mounting positions.

3. The culture dish rack fixing bracket according to claim 2, characterized in that: The first mounting portion and the second mounting portion are respectively arranged at an obtuse angle to the support fixing portion.

4. The culture dish rack fixing bracket according to claim 2, characterized in that: The mounting hole is a through hole structure or a U-shaped groove or an L-shaped groove.

5. The culture dish rack fixing bracket according to any one of claims 2 to 4, characterized in that: The rack fixing members are fixed at different height positions of the same first bracket; and / or The rack fixing parts are fixed at the same height positions of a plurality of the first brackets.

6. The culture dish rack fixing bracket according to any one of claims 2 to 4, characterized in that: The heights of the mounting holes on the two rack fixing members used to fix the same culture dish rack match, and the heights of the two rack fixing members are the same or different.

7. The culture dish rack fixing bracket according to claim 1, characterized in that: A row of mounting positions of the rack fixture is composed of two mounting holes, which are used to mount a small culture dish rack on one side of the rack fixture; or A row of mounting positions of the rack fixture is composed of more than four mounting holes, which are used to mount more than two small culture dish racks, or at least one large culture dish rack, on the left or right side of the rack fixture; or The lengths of the mounting fixtures of two adjacent first brackets are different, and the longer mounting fixture matches the height of the mounting hole on at least one shorter mounting fixture.

8. The culture dish rack fixing bracket according to claim 1, characterized in that: The electrical receptacle interface includes an electrical line connector having control pins.

9. The culture dish rack fixing bracket according to claim 8, characterized in that: The control pins in the electrical circuit connector include: Light source common cathode / common anode pins and / or Top light source pin 1 and / or Top mounted second light source pin and / or Top third light source pin and / or Top mounted 4th light source pin and / or Side light pins and / or Servo motor power pins and / or Servo motor ground pin and / or Servo motor data bus pins.

10. The culture dish rack fixing bracket according to claim 1, characterized in that: The first bracket is also provided with an air pump fixing plate, an air pump shock absorbing sleeve and an air pump; a plurality of the air pump shock absorbing sleeves are fixed on the air pump fixing plate, and the air pump is arranged inside the air pump shock absorbing sleeve; the air pump is used for gas exchange inside and outside the culture dish rack.

11. The culture dish rack fixing bracket according to claim 10, characterized in that: The pneumatic joint is connected to the gas pipeline of the air pump.

12. The culture dish rack fixing bracket according to claim 1, characterized in that: The rotating platform comprises: a base, a flange fixed to the base, a baffle and a photoelectric limit switch fixed to the base; The baffle is fixed at the gap of the flange, and the height of the baffle matches the height of the photoelectric limit switch; the baffle is provided with a notch, and the optical signal of the photoelectric limit switch can pass through the notch.

13. The culture dish rack fixing bracket according to claim 1, characterized in that: Also includes: A branching plate, fixed above the rotating platform; Wherein, a conductive slip ring is arranged inside the rotating platform, and the cables connected to the electrical socket interface are electrically connected to the conductive slip ring after being joined via the branching board.

14. The culture dish rack fixing bracket according to claim 13, characterized in that: The top of the rotating platform is provided with a mounting hole, through which the second bracket and the branching plate are fixed; the second bracket is fixed between the branching plate and the rotating platform; The rotating platform is also provided with a central through hole, and the cables connected to the electrical socket interface are electrically connected to the conductive slip ring through the central through hole after being joined via the branching board.

15. The culture dish rack fixing bracket according to claim 1, characterized in that: Also includes: The electrical equipment cover is fixed above the second bracket; the electrical equipment cover is provided with an air pump pipe outlet.

16. The culture dish rack fixing bracket according to claim 13, characterized in that: The liquid delivery joint is connected to the liquid pipeline of the conductive slip ring.

17. The culture dish rack fixing bracket according to claim 16, characterized in that: The liquid delivery connector is arranged on the side of the electrical socket interface.

18. The culture dish rack fixing bracket according to claim 14, characterized in that: The mounting holes include: a bracket mounting hole and a branching board mounting hole; the bracket mounting holes and the branching board mounting holes are arranged at intervals.

19. An incubator, characterized in that: The interior of the incubator is provided with a culture dish rack fixing bracket as described in any one of claims 1 to 18.

20. A biological sample morphology imaging device, characterized in that: include: The incubator and imaging device as described in claim 19; the imaging device is used to photograph the morphology of biological samples inside the incubator.

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