Culture dish rack fixing bracket, incubator and biological sample morphology imaging equipment

Abstract

The disclosed embodiment discloses a culture dish rack fixing bracket, an incubator, and a biological sample morphology imaging device, wherein the culture dish rack fixing bracket includes a rotating platform and a bracket body, wherein the bracket body is fixedly connected to the rotating platform, and the bracket body includes a first bracket and a second bracket, wherein the first bracket is radially distributed, and the second bracket is fixed to the end of the first bracket extending out of the rotating platform, and the space adjacent to the second bracket is used to fix the culture dish rack. This technical solution can fix multiple culture dish racks on the second bracket, and driven by the rotating platform, different culture dish racks can be respectively aligned with the imaging device set at the fixed position, so that the biological samples in multiple culture dish racks can be imaged and analyzed at the same time, thereby improving the sample analysis throughput.

Description

Technical Field

[0001] The present disclosure relates to the technical field of biological sample detection, and in particular to a culture dish rack fixing bracket, a culture box and a biological sample morphology imaging device. Background Art

[0002] In traditional biological research, due to the limitations of experimental methods, we can usually only study the static state of organisms at a certain time section, reflecting the cumulative effect of all biological dynamic processes before that time point. However, the growth and development of organisms is a combination of a series of time-space specific processes. Only by observing the dynamic change process can we more comprehensively understand the spatiotemporal specificity of biological growth and development regulation.

[0003] The prior art provides a device that needs to be placed in a dark greenhouse with a constant temperature to maintain the stability of the biological growth environment, and controls the movement of the camera to switch the camera field of view between samples to observe the growth and development of the organism under different temporal and spatial characteristics. However, the device cannot achieve simultaneous analysis of large-scale samples, and can only provide a single experimental environment, which cannot meet the requirements of applying a normal culture light source and controlling the most basic conditions for biological growth such as temperature, oxygen, and carbon dioxide. Summary of the invention

[0004] In order to solve the problems in the related art, the embodiments of the present disclosure provide a culture dish rack fixing bracket, a culture box and a biological sample morphology imaging device.

[0005] In a first aspect, an embodiment of the present disclosure provides a culture dish rack fixing bracket.

[0006] Specifically, the culture dish fixing bracket includes: a rotating platform and a bracket body, wherein the bracket body is fixedly connected to the rotating platform, and the bracket body includes a first bracket and a second bracket, the first bracket is radially distributed, and the second bracket is fixed to the end of the first bracket extending out of the rotating platform, and the space portion adjacent to the second bracket is used to fix the culture dish rack.

[0007] Optionally, the culture dish rack fixing bracket further comprises:

[0008] A branching plate, fixed above the rotating platform;

[0009] Among them, a conductive slip ring is arranged inside the rotating platform, and an electrical socket interface for electrically connecting to the culture dish rack is arranged on the second bracket. The cable connected to the electrical socket interface passes through the distribution board and is electrically connected to the conductive slip ring.

[0010] Optionally, a mounting hole is provided on the top of the rotating platform, and the first bracket and the branching plate are fixed through the mounting hole; the first bracket is fixed between the branching plate and the rotating platform;

[0011] The rotating platform is also provided with a central through hole, and the cable connected to the electrical socket interface passes through the distribution board and is electrically connected to the conductive slip ring through the central through hole.

[0012] Optionally, the mounting holes include: a bracket mounting hole and a branch board mounting hole; the bracket mounting holes and the branch board mounting holes are arranged at intervals.

[0013] Optionally, there are at least 2 groups of bracket mounting holes.

[0014] Optionally, the bracket mounting holes are provided in 8 groups, and the angle between each group is 45°.

[0015] Optionally, the conductive slip ring comprises: a stator part and a rotor part; the rotor part is fixed to the table top of the rotating platform; and the stator part is fixed to the base of the rotating platform.

[0016] Optionally, the rotating platform includes: a base, a flange fixed to the base, a baffle fixed to the flange, and a photoelectric limit switch;

[0017] The photoelectric limit switch is fixed on the base, and its height is adapted to the baffle; the baffle is provided with a notch, and the optical signal of the photoelectric limit switch can pass through the notch.

[0018] Optionally, a fixing piece is further provided on the second bracket for fixing the culture dish rack.

[0019] Optionally, the culture dish rack fixing bracket also includes: an air pump fixing plate, an air pump shock absorbing sleeve and an air pump; the air pump fixing plate is fixed above the branching plate, a number of the air pump shock absorbing sleeves are fixed on the air pump fixing plate, and the air pump is arranged in the air pump shock absorbing sleeve; the air pump is used for gas exchange inside and outside the culture dish rack.

[0020] Optionally, the culture dish rack fixing bracket further comprises:

[0021] A liquid pump is used to transport liquid into the culture dish rack.

[0022] Optionally, the culture dish rack fixing bracket further includes: an electrical equipment cover fixed above the first bracket; an air pump pipe outlet is provided on the electronic equipment cover, and an air pump pipe connected to the air pump is connected to the culture dish rack through the air pump pipe outlet.

[0023] Optionally, the culture dish rack fixing bracket further includes: a corner reinforcement block, which is arranged between adjacent first brackets and is used to reinforce the bracket body.

[0024] In a second aspect, an embodiment of the present disclosure provides an incubator.

[0025] Specifically, the incubator is fixed to the optical seismic isolation platform, and comprises: a culture dish rack fixing bracket and a box body as described in any one of the first aspects;

[0026] The bottom of the box body is provided with an opening, and the culture dish rack fixing bracket is fixed to the optical seismic isolation platform through the opening;

[0027] An observation window is installed on one side wall of the box body, and each culture dish rack can be observed through the observation window by rotating the culture dish rack fixing bracket.

[0028] Optionally, an aviation socket panel is installed on the box body to provide an electrical connection interface for the culture dish rack fixing bracket.

[0029] Optionally, the incubator further comprises: a temperature control device for providing a constant temperature environment for the incubator;

[0030] The temperature control device includes: a compressor, which is arranged outside the incubator.

[0031] Optionally, the incubator further comprises: an air inlet and an air outlet opened on the box body, and a solenoid valve located at the air outlet;

[0032] The air inlet is used to connect to the air supply component, and the air supply component is used to provide a gas environment for the growth of plants in the incubator; the solenoid valve is used to control the opening and closing state of the exhaust port.

[0033] Optionally, the observation window is coated on both sides to increase the transmittance of visible light and infrared light.

[0034] Optionally, heating elements are arranged around the observation window to prevent frost on the observation window glass.

[0035] In a third aspect, the embodiments of the present disclosure also provide a biological sample morphology imaging device.

[0036] Specifically, the biological sample morphology imaging device includes: an incubator and an imaging device as described in any one of the second aspects; the imaging device is used to photograph the morphology of the biological sample in the culture dish rack through the observation window.

[0037] Optionally, taking the plane where the observation window is located as the Z-axis direction in the three-dimensional space, the shooting component of the imaging device can move in the X-axis, Y-axis and Z-axis directions in the three-dimensional space.

[0038] The technical solution provided by the embodiments of the present disclosure may have the following beneficial effects:

[0039] The culture dish rack fixing bracket provided in the embodiment of the present disclosure can fix multiple culture dish racks on the second bracket. Driven by the rotating platform, different culture dish racks can be respectively aligned with imaging devices (such as CCD cameras or CMOS cameras) set at fixed positions, so that imaging analysis of biological samples in multiple culture dish racks can be performed simultaneously, thereby improving the sample analysis throughput.

[0040] It is to be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present disclosure. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Other features, objectives and advantages of the present disclosure will become more apparent through the following detailed description of non-limiting embodiments in conjunction with the accompanying drawings. In the accompanying drawings:

[0042] Figure 1 A front view of a culture dish rack fixing bracket according to an embodiment of the present disclosure is shown;

[0043] Figure 2 A schematic diagram showing a culture dish rack fixing bracket fixing a culture dish according to an embodiment of the present disclosure;

[0044] Figure 3 Show Figure 1 Schematic diagram of electrical equipment cover removed;

[0045] Figure 4 A top view of a culture dish rack fixing bracket according to an embodiment of the present disclosure is shown;

[0046] Figure 5 A schematic diagram showing the position relationship of a photoelectric limit switch according to an embodiment of the present disclosure;

[0047] Figure 6 A schematic diagram showing the structure of an incubator according to an embodiment of the present disclosure is shown;

[0048] Figure 7 A schematic structural diagram of a biological sample morphology imaging device according to an embodiment of the present disclosure is shown. DETAILED DESCRIPTION

[0049] Hereinafter, exemplary embodiments of the present disclosure will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily implement them. In addition, for the sake of clarity, parts not related to the description of the exemplary embodiments are omitted in the accompanying drawings.

[0050] In the present disclosure, it should be understood that terms such as "include" or "have" are intended to indicate the presence of features, numbers, steps, behaviors, components, parts, or a combination thereof disclosed in the present specification, and are not intended to exclude the possibility that one or more other features, numbers, steps, behaviors, components, parts, or a combination thereof exist or are added.

[0051] It should also be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present disclosure may be combined with each other. The present disclosure will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0052] The prior art provides a device that needs to be placed in a dark greenhouse with a constant temperature to maintain a stable plant growth environment, and controls the movement of the camera to switch the camera field of view between samples to observe the growth and development of organisms under different temporal and spatial characteristics. However, the device cannot achieve simultaneous analysis of large-scale samples, and can only provide a single experimental environment, which cannot meet the requirements of applying a normal culture light source and controlling the most basic conditions for biological growth such as temperature, oxygen, and carbon dioxide.

[0053] In basic scientific research in botany, including but not limited to plant resistance to environmental stress (resistance to high temperature, frost, salt, hypoxia, and oxidative damage), light signal pathways, photosynthesis, plant hormone signal pathways, plant developmental biology, plant breeding, etc., it is necessary to study dynamic temporal and spatial specificity, and therefore requires matching experimental techniques.

[0054] The present disclosure is made to at least partially solve the problems in the prior art discovered by the inventors.

[0055] like Figure 1-Figure 5 As shown, an embodiment of the present disclosure provides a culture dish rack fixing bracket, the culture dish rack fixing bracket 1 comprises: a rotating platform 11 and a bracket body 12, wherein the bracket body 12 is fixedly connected to the rotating platform 11, the bracket body 12 comprises a first bracket 121 and a second bracket 122, the first bracket 121 is radially distributed, the end of the first bracket 121 extending out of the rotating platform 11 is fixed with the second bracket 122, and the space portion adjacent to the second bracket 122 is used to fix the culture dish rack A.

[0056] The culture dish rack fixing bracket provided in the embodiment of the present disclosure can fix multiple culture dish racks on the second bracket. Driven by the rotating platform, different culture dish racks can be respectively aligned with imaging devices (such as CCD cameras or CMOS cameras) set at fixed positions, so that imaging analysis of biological samples in multiple culture dish racks can be performed simultaneously, thereby improving the sample analysis throughput.

[0057] According to an embodiment of the present disclosure, the culture dish rack fixing bracket also includes: a junction plate 13, fixed above the rotating platform 11, wherein a conductive slip ring 111 is arranged inside the rotating platform 11, and an electrical socket interface 123 for electrically connecting to the culture dish rack is arranged on the second bracket 122, and the cable connected to the electrical socket interface 123 is electrically connected to the conductive slip ring 111 after being converged via the junction plate 13.

[0058] In the manner of the embodiment of the present disclosure, a light source with different wavelengths and / or light intensities can be provided in the culture dish rack as a culture light source for biological samples. The electrical socket interface 123 is used to electrically connect to the light source in the culture dish rack, thereby providing light source conditions for the culture of biological samples. The number of electrical socket interfaces 123 can correspond to the number of culture dish racks, so as to facilitate the nearby connection of the electrical socket interfaces 123 and the culture dish racks. After the cables led out from the different second brackets 122 are merged via the branching plate 13, they are electrically connected to the conductive slip ring 111 inside the rotating platform 11, ensuring that the cables can rotate along with the culture dish rack during the rotation of the second bracket 122, without cable entanglement, and the electrical signals are reliably transmitted from the conductive slip ring 111 to the electrical socket interface 123.

[0059] According to an embodiment of the present disclosure, a mounting hole 112 is provided on the top of the rotating platform 11, and the first bracket 121 and the wiring board 13 are fixed through the mounting hole 112. The first bracket 121 is fixed between the wiring board 13 and the rotating platform 11. The rotating platform 11 is also provided with a central through hole 113, and the cables connected to the electrical socket port 123 are electrically connected to the conductive slip ring 111 through the central through hole 113 after being merged through the wiring board 13.

[0060] According to an embodiment of the present disclosure, the mounting holes 112 include: a bracket mounting hole 1121 and a branching board mounting hole 1122; the bracket mounting holes 1121 and the branching board mounting holes 1122 are arranged at intervals. The first bracket 121 is fixed through the bracket mounting holes 1121 to form a radial distribution, and the branching board 13 is fixed above the rotating platform 11 through the branching board mounting holes 1122. The first bracket 121 extends from the gap between the vertical mounting parts that fix the branching board 13 to the outside of the table of the rotating platform 11 to form a free end, and the second bracket 122 is installed on the free end.

[0061] According to an embodiment of the present disclosure, there are at least two groups of the bracket mounting holes 1121 , thereby forming at least one group of adjacent second brackets 122 , so as to facilitate fixing the culture dish rack in the space portion of the adjacent second brackets 122 .

[0062] According to an embodiment of the present disclosure, there are 8 groups of bracket mounting holes 1121, and the angle between each group is 45°, thereby forming an eight-way main bracket, that is, the number of second brackets 122 is 8, and there are 8 groups of adjacent second brackets 122 for fixing the culture dish rack.

[0063] Those skilled in the art will appreciate that the bracket mounting holes 112 may be in other groups, and the angles between each group may be other angles, so as to form a main bracket in other directions besides eight directions. The present disclosure does not limit this.

[0064] In the method of the embodiment of the present disclosure, each group of bracket mounting holes 1121 can be used to fix a group of first brackets 121, so there are 8 groups of first brackets 121 and second brackets 122. After the culture dish racks are fixed on the adjacent second brackets 122, for the imaging device at a fixed position, it can be rotated in 8 directions so that the culture dish racks in each direction are aligned with the imaging device, and then the imaging analysis of the biological sample is performed. It should be noted that multiple culture dish racks can be set at different heights on the second bracket 122. When performing imaging analysis, it is only necessary to adjust the height of the imaging device to image the biological sample in the culture dish rack without adjusting the alignment position of the culture dish rack.

[0065] In the embodiment of the present disclosure, the number of each group of bracket mounting holes 1121 can be 1-5, providing support for fixing the bracket body 12. The specific number can be flexibly adjusted as needed, and the present disclosure does not impose any restrictions on this.

[0066] According to an embodiment of the present disclosure, the conductive slip ring 111 includes: a stator part and a rotor part; the rotor part is fixed to the table top of the rotating platform 11 ; and the stator part is fixed to the base 114 of the rotating platform 11 .

[0067] According to an embodiment of the present disclosure, the rotating platform 11 includes: a base 114, a flange 115 fixed to the base 114, a baffle 116 fixed to the flange 115, and a photoelectric limit switch 117;

[0068] The photoelectric limit switch 117 is fixed on the base 114 , and its height is adapted to the baffle 116 ; the baffle 116 has a notch 1161 , and the optical signal of the photoelectric limit switch 116 can pass through the notch 1161 .

[0069] In the method of the embodiment of the present disclosure, the photoelectric limit switch 117 is used to calibrate the zero point position of the rotating platform 11. When the rotating platform 11 rotates, the baffle 116 can block the light signal of the photoelectric limit switch 117, and the light signal at the notch 1161 can pass through. The zero point position can be calibrated by detecting the light signal so that the culture dish rack can be aligned with the imaging device.

[0070] An exemplary calibration method is as follows:

[0071] a) Rotate counterclockwise at a speed of about 25° / s until the photoelectric limit switch signal is turned on and stops moving;

[0072] b) Rotate clockwise at a speed of about 10° / s until the photoelectric limit switch signal is disconnected and stops moving;

[0073] c) Rotate counterclockwise at a speed of about 0.1° / s until the photoelectric limit switch signal is turned on and stops moving;

[0074] d) Rotate 1° clockwise at normal drive speed and set the current position as the zero point.

[0075] The zero point position is calibrated by the above calibration method to achieve accurate positioning of the biological sample in the culture dish rack by the imaging device, which can improve the accuracy of biological sample analysis.

[0076] Those skilled in the art will appreciate that other clockwise or counterclockwise rotation methods and rotation speeds may be used to calibrate the zero point position, and the present disclosure does not limit this.

[0077] According to an embodiment of the present disclosure, a fixing member 124 is further provided on the second bracket 122 for fixing the culture dish rack.

[0078] According to an embodiment of the present disclosure, the culture dish rack fixing bracket further includes: a corner reinforcement block 125 disposed between adjacent first brackets 121 for reinforcing the bracket body 12 .

[0079] In the embodiment of the present disclosure, the corner reinforcement block 125 is disposed at a position of the first bracket 121 close to the second bracket 122 to ensure the stability of the bracket body 12 during the rotation process.

[0080] According to an embodiment of the present disclosure, the culture dish rack fixing bracket also includes: an air pump fixing plate 14, an air pump shock absorbing sleeve 15 and an air pump 16; the air pump fixing plate 14 is fixed above the branching plate 13, and a plurality of the air pump shock absorbing sleeves 15 are fixed on the air pump fixing plate 14, and the air pump 16 is arranged in the air pump shock absorbing sleeve 15; the air pump 16 is used for gas exchange inside and outside the culture dish rack.

[0081] In the embodiment of the present disclosure, air, oxygen, carbon dioxide, ethylene and other gases can be pumped into the culture dish rack by an air pump to study the growth status of plants under the experimental conditions, thereby meeting the needs of different experimental conditions.

[0082] According to an embodiment of the present disclosure, the culture dish rack fixing bracket also includes: an electrical equipment cover 17, fixed above the first bracket 121; an air pump tube outlet 171 is provided on the electronic equipment cover 17, and the air pump tube connected to the air pump 16 is connected to the culture dish rack through the air pump tube outlet 171.

[0083] According to an embodiment of the present disclosure, a liquid pump such as a peristaltic pump (not shown in the drawings) may also be provided on the air pump fixing plate 14, and the liquid conduit may be connected to the culture dish rack through the air pump tube outlet 171 to transport liquid such as nutrient solution to the culture dish.

[0084] The culture dish rack fixing bracket provided in the embodiment of the present disclosure can be fixed to the optical seismic isolation platform to prevent external vibration from affecting the alignment accuracy of the culture dish rack and the imaging device. When using the imaging device to perform imaging analysis on the biological samples in the culture dish rack, it is necessary to calibrate the zero point position of the rotating platform. After calibration, the rotation speed of the rotating platform can be controlled by a stepper motor or a direct drive motor. The rotating platform drives the bracket body to rotate so that the culture dish rack that needs to be imaged in each direction can be aligned with the imaging device at a fixed position, and then the imaging device is used to analyze the sample photos taken. The culture dish rack fixing bracket provided in the embodiment of the present disclosure can fix multiple culture dish racks, so that imaging analysis can be performed on biological samples in multiple culture dish racks at the same time, which improves the sample analysis throughput.

[0085] like Figure 6 As shown, the present disclosure provides a culture box, the culture box 2 can be fixed to the optical seismic isolation platform, including: a culture dish rack fixing bracket 1 and a box body 20. The culture dish rack fixing bracket is shown in FIG. Figure 1-Figure 5 The embodiments shown are not described in detail here.

[0086] The bottom of the box 20 is provided with an opening 21, and the culture dish rack fixing bracket is fixed to the optical seismic isolation platform through the opening 21 to avoid affecting the stability of the rotating platform 11 during operation due to insufficient mechanical strength of the box 20, and to avoid direct transmission of the vibration of the box 20 itself to the culture dish rack fixing bracket. A thin rubber pad can be added between the opening 21 and the optical seismic isolation platform to avoid damaging the air tightness of the box 20 here. An observation window 22 is installed on one of the side walls of the box 20, and the culture dish rack fixing bracket can be rotated, and each culture dish rack A can be observed through the observation window 22.

[0087] According to an embodiment of the present disclosure, an aviation socket panel 23 is installed on the box 20 to provide an electrical connection interface for the culture dish rack fixing bracket. The electrical connection between the internal equipment of the box 20 and the outside world is through the aviation socket on the panel to ensure that the wiring is light-proof and air-tight.

[0088] According to an embodiment of the present disclosure, the incubator 2 further includes: a temperature control device for providing a constant temperature environment for the incubator; the temperature control device includes: a compressor 24, which is arranged outside the incubator and connected to the box body 20 by a copper tube, so as to avoid the vibration generated by the compressor when working from being transmitted to the culture dish rack fixing bracket, thereby further improving the alignment accuracy of the biological sample morphology imaging device and the culture dish rack.

[0089] The temperature control device also includes: a condenser, an evaporator, a circulating fan, a temperature sensor, a control circuit and other parts. Except for the compressor 24, the other parts of the temperature control device are arranged in the box body 20, and a bottom long strip air outlet 25 is also provided in the box body 20. When the door of the box body 20 is closed, the air cooled or heated by the temperature control device is blown out from the bottom long strip air outlet 25 and sucked back from the top circulating fan to maintain a constant temperature environment in the enclosed space in the box body 20.

[0090] According to an embodiment of the present disclosure, an air inlet 26 and an exhaust port 27 are provided on the box body 20, as well as a solenoid valve located at the exhaust port 27; the air inlet 26 is used to connect to an air supply component, and the air supply component is used to provide a gas environment in the incubator; the solenoid valve is used to control the opening and closing state of the exhaust port 27.

[0091] In the embodiment of the present disclosure, the gas supply assembly connected to the air inlet 26 can provide an experimental environment such as air, oxygen, carbon dioxide, ethylene, etc. for the enclosed space in the box 20, and the air pump 16 can pump the gas in the box 20 into the corresponding culture dish rack to study the growth status of biological samples under the experimental environment.

[0092] It should be noted that in order to avoid the influence of the original gas in the box 20 on the experimental conditions (such as gas concentration), the exhaust port 27 is opened by the solenoid valve, and high-pressure air is introduced to quickly flush the gas in the box 20 and discharge it outside the box 20, and then the exhaust port 27 is closed by the solenoid valve, and the gas that meets the experimental conditions can be introduced into the box 20 through the air supply assembly. After closing the door, the inside of the box 20 remains strictly sealed, except for the observation window, it is opaque and airtight. When the solenoid valve at the exhaust port 27 is closed, the gas introduced from the air inlet 26 can generate a small positive pressure inside the box, which can prevent the external air from infiltrating from the possible gaps caused by the production and manufacturing process problems and polluting the internal environment.

[0093] According to the embodiments of the present disclosure, the imaging device on the outside of the box 20 can photograph the biological sample through the observation window 22. The glass used for the observation window 22 is double-sided coated, and the transmittance of the glass in the visible light and infrared light bands is increased at the same time, so as to reduce the reflection of the infrared lighting source on the outside of the box 20 into the lens, and reduce the loss of visible light signals of the biological samples in the box 20 when detected by the sensor on the outside of the box 20.

[0094] According to an embodiment of the present disclosure, heating elements are provided around the observation window 22 to prevent frost on the surface of the observation window glass when the internal low temperature environment is used.

[0095] like Figure 7 As shown, the present disclosure provides a biological sample morphology imaging device, which includes: a culture dish rack fixing support 1, a culture box 2, an optical seismic isolation platform 3 and an imaging device 4; the culture box is shown in FIG. Figure 6 The incubator 2 and the imaging device 4 are both fixed to the optical vibration isolation platform 2 .

[0096] The imaging device 4 is used to photograph the morphology of the biological sample in the culture dish rack through the observation window.

[0097] According to the embodiments of the present disclosure, with the plane where the observation window is located as the Z-axis direction in the three-dimensional space, the shooting component of the imaging device can move in the X-axis, Y-axis, and Z-axis directions in the three-dimensional space. By adjusting the displacement of the imaging device in the X-axis, Y-axis, and Z-axis directions, and rotating the corresponding culture dish rack A to align with the shooting component through the rotating platform, it is possible to image the biological samples in several culture dish racks A fixed on the culture dish rack fixing bracket, and then perform imaging analysis without adjusting the alignment position of the culture dish rack. It is also possible to image multiple plant samples in the same culture dish rack A, and autofocus can be achieved, thereby achieving high-throughput imaging.

[0098] The biological sample morphology imaging device provided by the embodiment of the present disclosure can be used for analyzing the growth status of plant samples, especially plant seedlings. By rotating the culture dish fixing bracket, a large number of plant seedling samples can be photographed simultaneously, thereby improving the analysis throughput. Moreover, while meeting the requirements of high-throughput imaging analysis, the temperature, gas component concentration, light and other conditions of the plant seedling culture environment in the culture dish rack can be flexibly adjusted, meeting the requirements of observing the changes in the growth of plant seedlings under a variety of experimental condition variables, thereby greatly expanding the function of the device.

[0099] The above description is only a preferred embodiment of the present disclosure and an explanation of the technical principles used. Those skilled in the art should understand that the scope of the invention involved in the present disclosure is not limited to the technical solution formed by a specific combination of the above technical features, but should also cover other technical solutions formed by any combination of the above technical features or their equivalent features without departing from the inventive concept. For example, the above features are replaced with the technical features with similar functions disclosed in the present disclosure (but not limited to) by each other.

Claims

1. A culture dish rack fixing bracket, characterized in that: include: A branching plate, a rotating platform and a bracket body, wherein the bracket body is fixedly connected to the rotating platform, the bracket body includes a first bracket and a second bracket, the first bracket is radially distributed, the second bracket is fixed to the end of the first bracket extending out of the rotating platform, and the space portion adjacent to the second bracket is used to fix the culture dish rack; the branching plate is fixed above the rotating platform; a conductive slip ring is arranged inside the rotating platform, and an electrical socket interface for electrically connecting to the culture dish rack is arranged on the second bracket, and the cable connected to the electrical socket interface is electrically connected to the conductive slip ring after passing through the branching plate; Wherein, the rotating platform includes: a base, a flange fixed to the base, a baffle fixed to the flange, and a photoelectric limit switch; the photoelectric limit switch is fixed on the base and its height is matched with the baffle; the baffle has a notch, and the optical signal of the photoelectric limit switch can pass through the notch.

2. The culture dish rack fixing bracket according to claim 1, characterized in that: The top of the rotating platform is provided with a mounting hole, through which the first bracket and the branching plate are fixed; the first bracket is fixed between the branching plate and the rotating platform; The rotating platform is also provided with a central through hole, and the cable connected to the electrical socket interface passes through the distribution board and is electrically connected to the conductive slip ring through the central through hole.

3. The culture dish rack fixing bracket according to claim 2, characterized in that: The mounting holes include: a bracket mounting hole and a branching board mounting hole; the bracket mounting holes and the branching board mounting holes are arranged at intervals.

4. The culture dish rack fixing bracket according to claim 3, characterized in that: The bracket mounting holes are at least 2 groups.

5. The culture dish rack fixing bracket according to claim 4, characterized in that: The bracket mounting holes are divided into 8 groups, and the angle between each group is 45°.

6. The culture dish rack fixing bracket according to claim 1, characterized in that: The conductive slip ring comprises: a stator part and a rotor part; the rotor part is fixed on the table surface of the rotating platform; and the stator part is fixed on the base of the rotating platform.

7. The culture dish rack fixing bracket according to any one of claims 1 to 6, characterized in that: The second bracket is also provided with a fixing piece for fixing the culture dish rack.

8. The culture dish rack fixing bracket according to claim 7, characterized in that: Also includes: An air pump fixing plate, an air pump shock absorbing sleeve and an air pump; the air pump fixing plate is fixed above the branching plate, a number of the air pump shock absorbing sleeves are fixed on the air pump fixing plate, and the air pump is arranged inside the air pump shock absorbing sleeve; the air pump is used for gas exchange inside and outside the culture dish rack.

9. The culture dish rack fixing bracket according to claim 8, characterized in that: Also includes: A liquid pump is used to transport liquid into the culture dish rack.

10. The culture dish rack fixing bracket according to claim 8, characterized in that: Also includes: The electrical equipment cover is fixed above the first bracket; the electrical equipment cover is provided with an air pump pipe outlet, and the air pump pipe connected to the air pump is communicated with the culture dish rack through the air pump pipe outlet.

11. The culture dish rack fixing bracket according to claim 1, characterized in that: Also includes: A corner reinforcement block is arranged between adjacent first brackets and is used to reinforce the bracket body.

12. An incubator, characterized in that: The incubator is fixed to the optical seismic isolation platform, comprising: a culture dish rack fixing bracket and a box body as described in any one of claims 1 to 11; The bottom of the box body is provided with an opening, and the culture dish rack fixing bracket is fixed to the optical seismic isolation platform through the opening; An observation window is installed on one side wall of the box body, and each culture dish rack can be observed through the observation window by rotating the culture dish rack fixing bracket.

13. The incubator according to claim 12, characterized in that: An aviation socket panel is installed on the box body to provide an electrical connection interface for the culture dish rack fixing bracket.

14. The incubator according to claim 12 or 13, characterized in that: Also includes: A temperature control device, used to provide a constant temperature environment for the incubator; The temperature control device includes: a compressor, which is arranged outside the incubator.

15. The incubator according to claim 14, characterized in that: Also includes: An air inlet and an air outlet are provided on the box body, and a solenoid valve is located at the air outlet; The air inlet is used to connect to the air supply component, and the air supply component is used to provide a gas environment for the growth of plants in the incubator; the solenoid valve is used to control the opening and closing state of the exhaust port.

16. The incubator according to claim 12, characterized in that: The observation window is coated on both sides to increase the transmittance of visible light and infrared light.

17. The incubator according to claim 16, characterized in that: Heating elements are arranged around the observation window to prevent the observation window glass from frosting.

18. A biological sample morphology imaging device, characterized in that: include: An incubator and an imaging device as described in any one of claims 12 to 17; the imaging device is used to photograph the morphology of biological samples in the culture dish rack through the observation window.

19. The device according to claim 18, characterized in that With the plane where the observation window is located as the Z-axis direction in the three-dimensional space, the shooting component of the imaging device can move in the X-axis, Y-axis and Z-axis directions in the three-dimensional space.

Images