PCR pipette clamping mechanism

Abstract

The present invention relates to the technical field of automated control equipment, and discloses a PCR liquid transfer gun clamping mechanism, including a clamping seat and a clamping head, the clamping head is installed with a gun seat, a clamping hole is arranged in the middle of the clamping seat, a driving shaft is arranged in the clamping hole, a bumping ball is arranged on the outer wall of the clamping hole, the upper end of the driving shaft is connected to a driving component, so that the driving shaft is displaced up and down, the size of the clamping head matches the clamping hole, a bumping ball hole is arranged on the outer diameter, the bumping ball hole corresponds to the bumping ball on the outer wall of the clamping hole, a gun head mounting part is provided below the gun seat, for installing the gun head, a piston rod is arranged in the gun seat, a lower magnet is arranged on the upper end of the piston rod, an upper magnet is arranged on the lower end of the driving shaft in the clamping hole, and the driving shaft drives the piston rod to move up and down through the suction force of the upper magnet and the lower magnet to realize the blowing and suction of the gun head. The present invention realizes rapid docking with a variety of row guns, and can blow and suck liquids and install and discard gun heads.

Description

Technical Field

[0001] The present invention relates to the technical field of laboratory-related equipment, and in particular to a PCR pipette clamping mechanism. Background Art

[0002] During the automated virus detection process, it is necessary to accurately take and put the liquid and replace the gun tip at any time. However, the existing technology does not have a particularly good solution and it can usually only be achieved through overly cumbersome equipment and steps. Summary of the invention

[0003] The purpose of the present invention is to solve the above problems and provide a PCR pipette clamping mechanism to achieve rapid docking with a variety of pipettes, and to be able to blow and aspirate liquids and install and discard gun tips.

[0004] The technical solution adopted by the present invention is:

[0005] A PCR pipette clamping mechanism, characterized in that it includes a clamping seat and a clamping head, the clamping head is installed with a gun seat, a clamping hole is arranged in the middle of the clamping seat, a driving shaft is arranged in the clamping hole, a bumping ball is arranged on the outer wall of the clamping hole, the upper end of the driving shaft is connected to the driving component to make the driving shaft move up and down, the size of the clamping head matches the clamping hole, a bumping ball hole is arranged on the outer diameter, the bumping ball hole corresponds to the bumping ball on the outer wall of the clamping hole, a gun head mounting part is provided below the gun seat for installing the gun head, a piston rod is arranged in the gun seat, a lower magnet is arranged on the upper end of the piston rod, an upper magnet is arranged on the lower end of the driving shaft in the clamping hole, and the driving shaft drives the piston rod to move up and down through the suction force of the upper magnet and the lower magnet to realize the blowing and suction of the gun head.

[0006] Furthermore, there are four beads, which are respectively installed on four positions of the outer wall of the clamping hole, and there are also four corresponding beads holes on the clamping head.

[0007] Furthermore, a shoulder is formed below the clamping head, a positioning column is provided on the lower end surface of the clamping seat, and a positioning hole is provided on the shoulder below the clamping head. When the clamping head and the clamping seat are clamped, they are positioned by the positioning column and the positioning hole.

[0008] Furthermore, the gun head mounting part is wrapped with a sleeve, and the sleeve is arranged on the outer diameter of the gun seat through a tension spring, and a vertical through groove is arranged in the clamping hole, the top edge of the sleeve is located at the through groove position, and the bottom edge of the sleeve is located above the connection between the gun head and the gun head mounting part, and a pressure plate is arranged on the driving shaft, and the pressure plate extends into the through groove and corresponds to the top edge of the sleeve, and the driving shaft is displaced downward to make the pressure plate cooperate with the sleeve, and the sleeve is displaced downward to push off the gun head.

[0009] Furthermore, the gun head mounting portion is provided with a conical head, and the conical head is sealingly matched with the head of the gun head.

[0010] Furthermore, support columns are symmetrically arranged on both sides of the shoulder of the clamping head, and the support columns are used for force support when the clamping seat is clamped or the clamping head is removed.

[0011] Furthermore, the clamping head is equipped with a gun tip mounting portion, and the gun tip mounting portion is equipped with gun tips ranging from 100ul to 1000ul.

[0012] Furthermore, the clamping head is equipped with four to eight gun tip mounting parts, and each gun tip mounting part is equipped with a 10ul to 20ul gun tip.

[0013] Furthermore, the clamping head is installed with four to eight gun seats, each gun seat is provided with a piston rod, the piston rod is connected to the piston parallel body, a lower magnet is provided on the piston parallel body, and the driving shaft drives each piston rod to move up and down through the suction force of the upper magnet and the lower magnet to realize the blowing and suction of the gun head.

[0014] Furthermore, the clamping head is installed with four to eight gun head mounting parts, each gun head mounting part is wrapped with a sleeve, and a parallel body is connected to the clamping seat. Each sleeve is arranged on the sleeve parallel body through a tension spring. The top edge of the sleeve parallel body is located at the through groove position. The driving shaft is displaced downward to make the pressure plate cooperate with the sleeve parallel body. The sleeve parallel body displaces the sleeve downward to push out the gun head of each gun head mounting part at the same time.

[0015] The beneficial effects of the present invention are:

[0016] (1) High versatility, enabling quick docking with single guns and volley guns;

[0017] (2) Automatically blow and suck liquid, and accurately control the blowing and suction volume through electrical circuits;

[0018] (3) Automatic loading and unloading of the gun tip. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Attached Figure 1 It is a three-dimensional structural schematic diagram of a single gun head of the present invention;

[0020] Attached Figure 2 It is a schematic diagram of the three-dimensional structure of the 8-row head of the present invention;

[0021] Attached Figure 3 It is a three-dimensional schematic diagram of the card seat structure;

[0022] Attached Figure 4 It is a three-dimensional schematic diagram of a single-gun chuck;

[0023] Attached Figure 5 It is a cross-sectional schematic diagram of a single-gun chuck;

[0024] Attached Figure 6 It is a three-dimensional schematic diagram of the eight-gun cartridge;

[0025] Attached Figure 7It is a cross-sectional schematic diagram of the eight-gun chuck.

[0026] The reference numerals in the accompanying drawings are:

[0027] 1. Card holder; 2. Card head;

[0028] 3. Mounting hole; 4. Driving shaft;

[0029] 5. Touch the bead; 6. Touch the bead hole;

[0030] 7. Gun head mounting part; 8. Conical head;

[0031] 9. Shoulder; 10. Positioning column;

[0032] 11. Positioning hole; 12. Support column;

[0033] 13. Piston rod; 14. Lower magnet;

[0034] 15. Upper magnet; 16. Sleeve;

[0035] 17. Tension spring; 18. Through slot;

[0036] 19. Pressure plate; 20. Piston parallel body;

[0037] 21. Sleeve parallel body. DETAILED DESCRIPTION

[0038] The specific implementation of the PCR pipette clamping mechanism of the present invention is described in detail below with reference to the accompanying drawings.

[0039] See attached Figure 1 , 2 The PCR pipette clamping mechanism is divided into two parts, one part is the clamping base 1, and the other part is the clamping head 2. The clamping head 2 is installed on the clamping base 1 to form a pipette. The clamping base 1 is displaced by a driving component, such as a mechanical arm or a robot, so as to send the pipette to a specified position for liquid operation.

[0040] See attached Figure 3 A mounting hole 3 is arranged in the middle of the mounting hole 3, a driving shaft 4 is arranged in the mounting hole 3, a bumping ball 5 is arranged on the outer wall of the mounting hole 3, and the bumping ball 5 can be arranged at four positions of the outer wall. The upper end of the driving shaft 4 is connected to the driving component to make the driving shaft 4 move up and down. The driving component is a stepping motor driving component, and precise displacement can be achieved through electrical control.

[0041] See attached Figure 4 , 5The size of the clamping head 2 matches the clamping hole 3, and a bead hole 6 is set on the outer diameter. The bead hole 6 corresponds to the bead 5 on the outer wall of the clamping hole 3. The position and number of the bead hole 6 correspond to the bead 5 on the base 1. Below the clamping head 2 is a gun head mounting part 7, and the gun head mounting part 7 is provided with a conical head 8. The conical head 8 is used to install the gun head and is sealed with the head of the gun head.

[0042] A shoulder 9 is formed below the clamp head 2, a positioning column 10 is provided on the lower end surface of the clamp base 1, and a positioning hole 11 is provided on the shoulder 9 below the clamp head 2. The clamp head 2 and the clamp base 1 are positioned by the positioning column 10 and the positioning hole 11 when they are assembled. Support columns 12 are symmetrically provided on both sides of the shoulder of the clamp head 2, and the support columns 12 are used for force support when the clamp base 1 is assembled or the clamp head 2 is disassembled.

[0043] Depending on the experimental requirements and liquid handling process needs, the cartridge 2 should be matched with different types of gun holders. For dispensing, use a single gun head of 100 to 1000ul, and for pipetting of 96-well plates, use a four-row or eight-row gun head of 10 to 20ul.

[0044] A piston rod 13 is arranged in the single gun head gun seat, a lower magnet 14 is arranged at the upper end of the piston rod 13, an upper magnet 15 is arranged at the lower end of the driving shaft 4 in the clamping hole 3, and the driving shaft 4 drives the piston rod 13 to move up and down through the suction force of the upper magnet 15 and the lower magnet 14 to realize the blowing and suction of the gun head.

[0045] The gun head mounting part 7 is wrapped with a sleeve 16, and the sleeve 16 is arranged on the outer diameter of the gun seat through a tension spring 17. A vertical through groove 18 is arranged in the clamping hole 3, and the top edge of the sleeve 16 is located at the position of the through groove 18. The bottom edge of the sleeve 16 is located above the connection between the gun head and the gun head mounting part 7. A pressure plate 19 is arranged on the driving shaft 4, and the pressure plate 19 extends into the through groove 18 and corresponds to the top edge of the sleeve 16. The driving shaft 4 is displaced downward to make the pressure plate 19 cooperate with the sleeve 16, and the sleeve 16 is displaced downward to push the gun head off.

[0046] See attached Figure 6 , 7 The clamping head 2 of the multi-gun head is installed with four to eight gun head mounting parts 7, and the clamping head 2 is installed with four to eight gun seats. A piston rod 13 is arranged in each gun seat, and the piston rod 13 is connected to the piston parallel body 20. A lower magnet 14 is arranged on the piston parallel body 20. The driving shaft 4 drives each piston rod 13 to move up and down through the suction force of the upper magnet 15 and the lower magnet 14 to realize the blowing and suction of the gun head.

[0047] The clamping head 2 is installed with four to eight gun head mounting parts 7, each gun head mounting part 7 is wrapped with a sleeve 16, and a sleeve parallel body 21 is connected to the clamping base 1. Each sleeve 16 is set on the sleeve parallel body 21 through a tension spring 17. The top edge of the sleeve parallel body 21 is located at the position of the through groove 18. The driving shaft 4 is displaced downward to make the pressure plate 19 cooperate with the sleeve parallel body 21. The sleeve parallel body 21 displaces the sleeve 16 downward to simultaneously push off the gun heads of each gun head mounting part 7.

[0048] When using a pipette, if you choose a single-gun head holder, move the holder to the gun head tray position, and install the single-gun head holder from the gun head tray onto the holder. Then install the gun head and perform the pipetting operation. After completion, remove the gun head through the sleeve.

[0049] When replacing the 8-gun head gun holder, first unclamp the single-gun head gun holder through the gun head plate, and then clamp the 8-gun head gun holder to perform liquid operations.

[0050] The above are only preferred embodiments of the present invention. It should be pointed out that ordinary technicians in this technical field can make several improvements and modifications without departing from the principles of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A PCR pipette clamping mechanism, characterized in that: The invention comprises a card seat and a card head, wherein the card head is installed with a gun seat, a card hole is arranged in the middle of the card seat, a driving shaft is arranged in the card hole, a bumping ball is arranged on the outer wall of the card hole, the upper end of the driving shaft is connected to the driving component to make the driving shaft move up and down, the size of the card head matches the card hole, a bumping ball hole is arranged on the outer diameter, the bumping ball hole corresponds to the bumping ball on the outer wall of the card hole, a gun head mounting part is arranged below the gun seat for mounting the gun head, a piston rod is arranged in the gun seat, a lower magnet is arranged on the upper end of the piston rod, an upper magnet is arranged on the lower end of the driving shaft in the card hole, and the driving shaft is connected to the driving component by the upper magnet and the lower magnet. The suction force of the iron drives the piston rod to move up and down to achieve blowing and suction of the gun head. The gun head mounting part is wrapped with a sleeve, and the sleeve is set on the outer diameter of the gun seat through a tension spring. A vertical through groove is set in the clamping hole, and the top edge of the sleeve is located at the through groove position, and the bottom edge of the sleeve is located above the connection between the gun head and the gun head mounting part. A pressure plate is set on the driving shaft, and the pressure plate extends into the through groove and corresponds to the top edge of the sleeve. The driving shaft is displaced downward to make the pressure plate cooperate with the sleeve, and the sleeve is displaced downward to push the gun head off. A conical head is set on the gun head mounting part, and the conical head is sealed and matched with the head of the gun head.

2. The PCR pipette clamping mechanism according to claim 1, characterized in that: There are four beads, which are respectively installed on four positions of the outer wall of the clamping hole, and there are also four corresponding beads holes on the clamping head.

3. The PCR pipette clamping mechanism according to claim 2, characterized in that: A shoulder is formed below the clamping head, a positioning column is arranged on the lower end surface of the clamping seat, and a positioning hole is arranged on the shoulder below the clamping head. When the clamping head and the clamping seat are clamped, they are positioned through the positioning column and the positioning hole.

4. The PCR pipette clamping mechanism according to claim 3, characterized in that: Support columns are symmetrically arranged on both sides of the shoulder of the clamping head, and the support columns are used for force support when the clamping seat is clamped or the clamping head is disassembled.

5. The PCR pipette clamping mechanism according to any one of claims 1 to 3, characterized in that: The clamping head is equipped with a gun tip mounting portion, and the gun tip mounting portion is equipped with a gun tip of 100ul to 1000ul.

6. The PCR pipette clamping mechanism according to any one of claims 1 to 3, characterized in that: The clamping head is equipped with four to eight gun tip mounting parts, and each gun tip mounting part is equipped with a 10ul to 20ul gun tip.

7. The PCR pipette clamping mechanism according to any one of claims 1 to 3, characterized in that: The clamping head is installed with four to eight gun seats, each gun seat is provided with a piston rod, the piston rod is connected to the piston parallel body, a lower magnet is provided on the piston parallel body, and the driving shaft drives each piston rod to move up and down through the suction force of the upper magnet and the lower magnet to realize the blowing and suction of the gun head.

8. The PCR pipette clamping mechanism according to claim 1, characterized in that: The clamping head is installed with four to eight gun head mounting parts, each gun head mounting part is wrapped with a sleeve, and a parallel body is connected to the clamping seat. Each sleeve is arranged on the sleeve parallel body through a tension spring. The top edge of the sleeve parallel body is located at the through groove position. The driving shaft is displaced downward to make the pressure plate cooperate with the sleeve parallel body. The sleeve parallel body displaces the sleeve downward to push off the gun head of each gun head mounting part at the same time.

Images