A cell culture device with variable volume

Abstract

The present invention provides a cell culture device with variable volume, including a base, a lower sleeve, an upper sleeve, a lifting frame, a cover body and a culture bag. The center of the lifting frame has a mounting hole that penetrates the lifting frame, and the hole wall of the mounting hole is provided with an annular slot. The cover body is mounted in the annular slot in cooperation. The culture bag is an upper-opening bag body, and the upper edge of the culture bag is sealed and pressed in the annular slot by the cover body. Two vertical guide components are provided on the base, and both sides of the lifting frame can be slidably mounted on the vertical guide rails of the vertical guide components on the corresponding side, and the driving device of each vertical guide component drives the lifting frame to rise and fall along the vertical guide rails of the vertical guide components. The present invention has a simple structure and is easy to manufacture. When culturing cells, the driving device drives the lifting frame to gradually rise to expand the volume according to the cell density, without changing the container, which greatly reduces the manual operation process.

Description

Technical Field

[0001] The invention relates to a cell culture device with changeable volume, belonging to the technical field of cell culture devices. Background Art

[0002] Conventional cell culture involves expanding cells into increasingly larger containers to hold space for the increasing number of cells. This step-by-step approach typically takes the form of expanding the cells of interest initially from a small sample in a vial into a large-volume cell bag or tank. During the cell expansion phase, cells are initially injected into flasks or spinner bottles to regenerate the cell line. Subsequently, the cells are transferred and expanded in cell bags on a shaker or in a stirred tank. During the expansion process, cells are gradually transferred to the next larger-sized container, such as a flask, and continued to expand.

[0003] Traditional cell culture methods require maintaining a certain cell density, so the volume of the culture container cannot be too large, otherwise the cell density will be too low and unable to grow; however, when the cells proliferate to a certain number and exceed a certain density, the overly dense cells will stop growing or even age and die. At this time, a larger volume culture container must be replaced, which requires very careful operation techniques to avoid cell contamination, which is very inconvenient and prone to failure. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention aims to provide a cell culture device with variable volume, so as to at least solve the technical problem of needing to replace culture containers of different volumes proposed in the above background technology.

[0005] In order to achieve the above-mentioned purpose, the present invention is realized through the following technical scheme: a cell culture device with variable volume, comprising a base, a lower sleeve, an upper sleeve, a lifting frame, a cover body and a culture bag, wherein the upper sleeve and the lower sleeve are both cylindrical parts with openings at the top and the bottom, the upper end of the upper sleeve is fixedly connected to the lifting frame, the lower end of the lower sleeve is fixedly connected to the base, the lower end of the upper sleeve is sleeved on the outside of the lower sleeve, the center of the lifting frame has a mounting hole that passes through the lifting frame, the hole wall of the mounting hole is provided with an annular groove, the cover body is cooperatively installed in the annular groove, and the culture bag is The bag body is opened on the top, and the upper edge of the culture bag is sealed and pressed into the annular groove by the cover body, so that the upper end of the culture bag is fixed by the cover body and the lifting frame, and the upper opening of the culture bag is sealed by the cover body. Two vertical guide components are arranged on the base, and the two vertical guide components are arranged on both sides of the lifting frame. Each vertical guide component includes a bracket, a vertical guide rail fixedly arranged on the bracket and a driving device. Both sides of the lifting frame can be slidably installed on the vertical guide rails on the corresponding side, and the driving device of each vertical guide component drives the lifting frame to rise and fall along the vertical guide rails of the vertical guide component.

[0006] Furthermore, there is a clearance fit between the lower sleeve and the upper sleeve to allow the upper sleeve to rise and fall freely. A rubber transition piece is fixedly provided on the upper end surface of the lower sleeve, and the rubber transition piece closes the gap between the upper sleeve and the lower sleeve to prevent the culture bag from being stuck in the gap between the upper sleeve and the lower sleeve during the lifting and lowering of the upper sleeve.

[0007] Preferably, the thickness of the rubber transition piece gradually decreases from bottom to top, and the upper end of the rubber transition piece is pressed against the inner wall of the upper sleeve, so that the gap between the upper sleeve and the lower sleeve is closed by the rubber transition piece.

[0008] Preferably, it further comprises a magnetic metal support plate disposed in the culture bag, the magnetic metal support plate being used to flatten the bottom surface of the culture bag, and a magnet for adsorbing the magnetic metal support plate is disposed on the upper surface of the base located directly below the culture bag.

[0009] Optionally, the upper sleeve and the lifting frame are connected by welding or fixedly connected by a connecting piece or are integrally formed.

[0010] Optionally, the lower sleeve and the base are connected by welding or fixedly connected by a connecting piece or are integrally formed.

[0011] Preferably, the driving device includes a driving motor, a screw rod rotatably mounted on a bracket and a threaded hole arranged on a lifting frame. The screw rod extends in a vertical direction, is passed through the threaded hole and cooperates with the threaded hole through threads. The motor shaft of the driving motor is connected to the screw rod for transmission, and the driving motor drives the screw rod to rotate forward or reversely, and then the screw rod drives the lifting frame to rise or fall through the threads.

[0012] Preferably, the bracket is fixedly arranged above the lifting frame, and the bracket is fixedly connected to the base through a support member. Optionally, the support member is a vertical guide rail and a screw rod.

[0013] Beneficial effects of the present invention:

[0014] The invention has a simple structure and is easy to manufacture. When culturing cells, the driving device drives the lifting frame to gradually rise to expand the volume according to the cell density, without replacing the container, which greatly reduces the manual operation process. At the same time, the cells can maintain a certain density and continue to grow without the need for operating technology, and the cells will not be contaminated. It is convenient and has a high success rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Other features, objects and advantages of the present invention will become more apparent from the detailed description of non-limiting embodiments made with reference to the following drawings:

[0016] Figure 1 It is a schematic structural diagram of a cell culture device with variable volume according to the present invention;

[0017] Figure 2 The figure is a schematic diagram of the top view of the structure of a cell culture device with variable volume according to the present invention. DETAILED DESCRIPTION

[0018] In order to make the technical means, creative features, objectives and effects of the present invention easy to understand, the present invention is further described below in conjunction with specific embodiments. The preferred embodiments of the present invention are shown in the accompanying drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. The purpose of providing these embodiments is to make the disclosure of the present invention more thoroughly and comprehensively understood.

[0019] It should be noted that when an element is referred to as being "disposed on" another element, it may be directly on the other element or there may be a centered element. When an element is considered to be "connected" to another element, it may be directly connected to the other element or there may be a centered element at the same time. The terms "center", "longitudinal", "lateral", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like used herein indicate an orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or component referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention, and such expressions are only for illustrative purposes and do not represent the only implementation method.

[0020] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0021] See also Figure 1-Figure 2The present invention provides a technical solution: a cell culture device with variable volume, comprising a base 1, a lower sleeve 2, an upper sleeve 3, a lifting frame 4, a cover body 5 and a culture bag 6, wherein the upper sleeve 3 and the lower sleeve 2 are both cylindrical parts with openings at the top and the bottom, the upper end of the upper sleeve 3 is fixedly connected to the lifting frame 4, the lower end of the lower sleeve 2 is fixedly connected to the base 1, the lower end of the upper sleeve 3 is sleeved on the outside of the lower sleeve 2, the center of the lifting frame 4 has a mounting hole that penetrates the lifting frame 4, the hole wall of the mounting hole is provided with an annular groove, the cover body 5 is cooperatively installed in the annular groove, and the culture bag 6 is a bag body with an upper opening The upper edge of the culture bag 6 is sealed and pressed into the annular groove by the cover body 5, so that the upper end of the culture bag 6 is fixed by the cover body 5 and the lifting frame 4, and the upper opening of the culture bag 6 is sealed by the cover body 5. Two vertical guide assemblies are arranged on the base 1, and the two vertical guide assemblies are arranged on both sides of the lifting frame 4. Each vertical guide assembly includes a bracket 7, a vertical guide rail 8 fixedly arranged on the bracket 7, and a driving device. Both sides of the lifting frame 4 can be slidably installed on the vertical guide rail 8 on the corresponding side, and the driving device of each vertical guide assembly drives the lifting frame 4 to rise and fall along the vertical guide rail 8 of the vertical guide assembly.

[0022] Furthermore, there is a clearance fit between the lower sleeve 2 and the upper sleeve 3 to allow the upper sleeve 3 to rise and fall freely. A rubber transition piece 9 is fixedly provided on the upper end surface of the lower sleeve 2, and the rubber transition piece 9 closes the gap between the upper sleeve 3 and the lower sleeve 2 to prevent the culture bag 6 from being stuck in the gap between the upper sleeve 3 and the lower sleeve 2 during the lifting and lowering of the upper sleeve 3.

[0023] Preferably, the thickness of the rubber transition piece 9 gradually decreases from bottom to top, and the upper end of the rubber transition piece 9 is pressed against the inner wall of the upper sleeve 3, so that the rubber transition piece 9 closes the gap between the upper sleeve 3 and the lower sleeve 2.

[0024] Preferably, it further comprises a magnetic metal support plate 10 disposed in the culture bag 6 , the magnetic metal support plate 10 is used to flatten the bottom surface of the culture bag 6 , and the upper surface of the base 1 located directly below the culture bag 6 is provided with a magnet 11 for adsorbing the magnetic metal support plate 10 .

[0025] Optionally, the upper sleeve 3 and the lifting frame 4 are connected by welding or fixedly connected by a connecting piece or are integrally formed.

[0026] Optionally, the lower sleeve 2 and the base 1 are connected by welding or fixedly connected by a connecting piece or are integrally formed.

[0027] Preferably, the driving device comprises a driving motor 12, a screw rod 13 rotatably mounted on the bracket 7 and a threaded hole provided on the lifting frame 4, the screw rod 13 extending in a vertical direction, the screw rod 13 passing through the threaded hole and passing through the threaded hole.

[0028] In cooperation with the threaded hole, the motor shaft of the driving motor 12 is connected to the screw rod 13 for transmission, and the driving motor 12 drives the screw rod 13 to rotate forward or reversely, and then the screw rod 13 drives the lifting frame 4 to rise or fall through the thread.

[0029] Preferably, the bracket 7 is fixedly arranged above the lifting frame 4, and the bracket 7 is fixedly connected to the base 1 through a supporting member. Optionally, the supporting member is a vertical guide rail 8 and a screw rod 13.

[0030] The invention has a simple structure and is easy to manufacture. When culturing cells, the driving device drives the lifting frame 4 to gradually rise according to the cell density, and there is no need to replace the container, which greatly reduces the manual operation process. At the same time, the cells can maintain a certain density and continue to grow without requiring any operation skills, and the cells will not be contaminated. It is convenient and has a high success rate.

[0031] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0032] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementations that can be understood by those skilled in the art.

Claims

1. A cell culture device with variable volume, characterized in that: The invention comprises a base, a lower sleeve, an upper sleeve, a lifting frame, a cover body and a culture bag. The upper sleeve and the lower sleeve are both cylindrical parts with openings at the top and the bottom. The upper end of the upper sleeve is fixedly connected to the lifting frame, the lower end of the lower sleeve is fixedly connected to the base, and the lower end of the upper sleeve is sleeved on the outside of the lower sleeve. The center of the lifting frame has a mounting hole penetrating the lifting frame, and the hole wall of the mounting hole is provided with an annular groove. The cover body is cooperatively installed in the annular groove. The culture bag is a bag body with an upper opening. The upper edge of the culture bag is sealed and pressed in the annular groove by the cover body, so that the upper end of the culture bag is fixed by the cover body and the lifting frame, and the upper opening of the culture bag is sealed by the cover body. Two vertical guide assemblies are arranged on the base, and the two vertical guide assemblies are respectively arranged on both sides of the lifting frame. Each vertical guide assembly comprises a bracket, a vertical guide rail fixedly arranged on the bracket and a driving device. Both sides of the lifting frame can be slidably installed on the vertical guide rails on the corresponding side, and the driving device of each vertical guide assembly drives the lifting frame to rise and fall along the vertical guide rails of the vertical guide assembly. The driving device comprises a driving motor, a screw rod rotatably mounted on the bracket and a threaded hole arranged on the lifting frame, the screw rod extends in a vertical direction, the screw rod is inserted into the threaded hole and matched with the threaded hole through a thread, the motor shaft of the driving motor is connected and driven by the screw rod, and the screw rod is driven by the driving motor to rotate forward or reversely; The lower sleeve and the upper sleeve are clearance-matched, and a rubber transition piece is fixedly provided on the upper end surface of the lower sleeve, and the gap between the upper sleeve and the lower sleeve is closed by the rubber transition piece.

2. The cell culture device with variable volume according to claim 1, characterized in that: The thickness of the rubber transition piece gradually decreases from bottom to top, and the upper end of the rubber transition piece is pressed against the inner wall of the upper sleeve.

3. The cell culture device with variable volume according to claim 1, characterized in that: It also includes a magnetic metal support plate arranged in the culture bag, the magnetic metal support plate is used to flatten the bottom surface of the culture bag, and the upper surface of the base located directly below the culture bag is provided with a magnet for adsorbing the magnetic metal support plate.

4. The cell culture device with variable volume according to claim 1, characterized in that: The upper sleeve and the lifting frame are connected by welding or fixedly connected by a connecting piece or are integrally formed.

5. The cell culture device with variable volume according to claim 1, characterized in that: The lower sleeve and the base are connected by welding or fixedly connected by a connecting piece or are integrally formed.

6. The cell culture device with variable volume according to claim 1, characterized in that: The bracket is fixedly arranged above the lifting frame, and the bracket is fixedly connected to the base through a supporting piece.

7. The cell culture device with variable volume according to claim 6, characterized in that: The supporting parts are vertical guide rails and screw rods.

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