Cell sorting device and cell sorting and concentration system

Abstract

The present invention relates to the field of biomedical instrument technology, and in particular to a cell sorting device and a cell sorting and concentrating system. The cell sorting device comprises a liquid storage barrel, a pipette assembly and a pressing assembly. The pipette assembly comprises a pipette and a plug body. The liquid storage barrel is provided with a first through hole. The plug body is arranged at the first through hole. The plug body is constructed with a second through hole extending along the axial direction of the liquid storage barrel. The pipette passes through the second through hole and enters the liquid storage barrel. The plug body comprises a telescopic section. One end of the telescopic section is connected to the liquid storage barrel, and the other end is fixedly connected to the pipette. The pressing assembly can drive the telescopic section to telescope along the axial direction of the liquid storage barrel to drive the pipette to move. Through the cooperation of the pressing assembly and the pipette assembly, fully enclosed and fully automated cell sorting or concentration can be realized, replacing the manual sorting or concentration of the prior art, improving the sorting or concentration efficiency and accuracy, and avoiding the contamination of the cell solution by the outside world during the sorting or concentration process.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedical instruments, and in particular to a cell sorting device and a cell sorting and concentrating system. Background Art

[0002] In the field of cell medicine, it is often necessary to directly or in vitro culture cells collected from living bodies and then inoculate them into patients. In order to use cells collected or cultured from living bodies for treatment, it is necessary to separate the cells from the blood, i.e., to sort the cells into PBMCs (peripheral blood mononuclear cells), and at the same time, it is necessary to remove impurities and culture medium and concentrate them into a cell concentration suitable for treatment. In order to achieve the purpose of PBMC sorting and concentration, centrifugation is generally used for the operation.

[0003] Conventional equipment in the prior art generally performs cell PBMC sorting and concentration during centrifugation, which can easily cause cell damage. The concentration volume that can be achieved is also small, generally around 300-400 ml, and rarely exceeds 1 L. It is also easy to cause contamination during manual operation, and the sorting and concentration efficiency is low. Summary of the invention

[0004] The present invention provides a cell sorting device and a cell sorting and concentrating system, which are used to solve the defects of the manual sorting process in the prior art that it is easy to cause pollution and the sorting efficiency is low. The pressing component and the straw component are combined to replace the manual sorting of the prior art, thereby improving the sorting efficiency and accuracy, and avoiding the contamination of the cell solution by the outside world during the sorting process.

[0005] The present invention provides a cell sorting device, comprising a liquid storage barrel, a straw assembly and a pressing assembly, the straw assembly comprising a straw and a plug body, the liquid storage barrel is provided with a first through hole, the plug body is arranged at the first through hole, the plug body is structured with a second through hole extending along the axial direction of the liquid storage barrel, the straw passes through the second through hole and enters the liquid storage barrel, the plug body comprises a telescopic section, one end of the telescopic section is connected to the liquid storage barrel, and the other end is fixedly connected to the straw, the pressing assembly can drive the telescopic section to telescope along the axial direction of the liquid storage barrel to drive the straw to move.

[0006] According to a cell sorting device provided by the present invention, the plug body further includes a fixed section, and the fixed section is coaxially arranged with the telescopic section.

[0007] According to a cell sorting device provided by the present invention, the telescopic section includes a telescopic member and a first end plate, one end of the telescopic member abuts against the liquid storage barrel, and the other end is connected to the first end plate, and the suction tube passes through the first end plate and is fixedly connected to the first end plate.

[0008] According to a cell sorting device provided by the present invention, the telescopic member is sleeved on the outside of the fixed section, and the fixed section is connected to the first end plate.

[0009] According to a cell sorting device provided by the present invention, a sealing ring is provided between the fixing section and the first through hole.

[0010] According to a cell sorting device provided by the present invention, the telescopic section includes an annular second end plate, the inner side of the second end plate is connected to the fixed section, one end surface of the second end plate is connected to the telescopic member, and the other end surface abuts against the liquid storage barrel.

[0011] According to a cell sorting device provided by the present invention, the pipette assembly further comprises a third end plate for contacting the pressing assembly, the third end plate is arranged on the plug body, and there is a gap between the plug body and the third end plate for the pipette to pass through.

[0012] A cell sorting device provided according to the present invention also includes a support base and a driving member, the support base is horizontally arranged, the liquid storage barrel is rotatably connected to the support base through a rotating shaft, the rotating shaft is horizontally arranged and perpendicular to the axial direction of the liquid storage barrel, and the driving member is connected to the support base to drive the support base to rotate around a rotation axis perpendicular to the axial direction of the rotating shaft.

[0013] According to a cell sorting device provided by the present invention, the support seat is provided with a liquid passage tube, and the suction tube is connected to the liquid passage tube.

[0014] According to a cell sorting device provided by the present invention, the liquid tube is coaxially arranged with the support seat, the upper end of the liquid tube is connected with the pipeline through a connecting sleeve, a bearing for connecting with the pipeline is provided in the connecting sleeve, and a sealing ring is provided between the pipeline and the inner wall of the connecting sleeve.

[0015] According to a cell sorting device provided by the present invention, a third through hole is provided on the upper part of the barrel wall of the liquid storage barrel on one side away from the rotation axis of the support seat, the third through hole is connected to the interior of the liquid storage barrel, and a breathable membrane is provided at the third through hole.

[0016] According to a cell sorting device provided by the present invention, the support seat is configured with a groove sunken from the edge toward the rotation axis of the support seat, and the liquid storage barrel is disposed in the groove.

[0017] The present invention also provides a cell sorting and concentration system, comprising a control device, a liquid supply device and the cell sorting device as described above, wherein the liquid supply device and the driving member are both connected to the control device, the liquid supply device comprises a bracket and a liquid channel, the bracket is used to place a liquid bag, and the liquid channel is connected to the liquid storage barrel.

[0018] The cell sorting device provided by the present invention has a liquid storage barrel placed vertically, and a centrifugally layered cell solution to be sorted is contained therein. A first through hole is provided on the top of the liquid storage barrel, and a plug body is installed at the first through hole. The plug body has a second through hole that is connected to the inside of the liquid storage barrel. The part of the plug body located outside the liquid storage barrel is a telescopic section. The telescopic section can be compressed when under pressure, and will stretch back to its original state when the pressure disappears. The straw is first inserted from the outside through the second through hole part in the telescopic section, and then through the second through hole part in the plug body located inside the liquid storage barrel, and finally enters to reach the inside of the liquid storage barrel, while the straw is fixed to the telescopic section. The pressing component moves downward to press the telescopic section, and the compression of the telescopic section drives the straw to move downward along the axial direction of the liquid storage barrel at the same time. The pressing component presses the telescopic section to different degrees, so that the end of the straw can reach the cell solution of different layers in the liquid storage barrel. By controlling the vertical compression distance of the telescopic section, the moving distance of the straw is controlled to perform PBMC sorting. After the PBMC sorting is completed, the pressing component moves upward and gradually leaves the telescopic section. The telescopic section stretches and recovers while driving the straw to move upward along the axis of the liquid storage barrel. The straw returns to its initial uncompressed state under the action of the telescopic section. This completes a complete cell PBMC sorting process, and the PBMC sorting can be repeated multiple times. The cooperation of the pressing component and the straw component replaces the manual sorting of the existing technology, improves the sorting efficiency and accuracy, and avoids the contamination of the cell solution by the outside world during the sorting process.

[0019] In addition to the technical problems solved by the present invention, the technical features of the technical solutions constituted, and the advantages brought about by the technical features of these technical solutions described above, other technical features of the present invention and the advantages brought about by these technical features will be further explained in conjunction with the accompanying drawings or will be understood through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 is a schematic longitudinal section diagram of the cell sorting device provided by the present invention in a static state;

[0022] Figure 2 It is a schematic structural diagram of a sealing ring of a cell sorting device provided by the present invention;

[0023] Figure 3 is a schematic structural diagram of a cell sorting device provided by the present invention in a static state;

[0024] Figure 4 is a longitudinal cross-sectional schematic diagram of the centrifugal state of the cell sorting device provided by the present invention;

[0025] Figure 5 It is a structural schematic diagram of the centrifugal state of the cell sorting device provided by the present invention;

[0026] Figure 6 This is one of the structural schematic diagrams of the pipette assembly of the cell sorting device provided by the present invention;

[0027] Figure 7 This is the second structural schematic diagram of the pipette assembly of the cell sorting device provided by the present invention;

[0028] Figure 8 This is the third structural schematic diagram of the pipette assembly of the cell sorting device provided by the present invention;

[0029] Fig. 9 It is a schematic structural diagram of a connecting sleeve of a cell sorting device provided by the present invention;

[0030] Fig.10 It is a schematic diagram of the structure of the cell sorting and concentration system provided by the present invention;

[0031] Reference numerals:

[0032] 100: cell sorting device;

[0033] 200: support seat; 210: liquid passage pipe; 220: groove; 230: connecting sleeve; 240: bearing; 250: sealing ring;

[0034] 300: liquid storage barrel; 310: first through hole; 330: third through hole; 340: air permeable membrane; 360: first liquid layer; 370: second liquid layer; 380: third liquid layer;

[0035] 400: pressing assembly; 410: pressing plate; 420: pressing rod;

[0036] 500: straw assembly; 510: straw; 520: plug body; 530: sealing ring; 540: third end plate; 550: connecting rod; 521: second through hole; 522: telescopic section; 523: fixed section; 5221: telescopic member; 5222: first end plate; 5224: second end plate;

[0037] 600: pipeline; 700: control device; 800: bracket; 900: liquid channel. DETAILED DESCRIPTION

[0038] The following embodiments of the present invention are described in further detail in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0039] In the description of the embodiments of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the embodiments of the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limitations on the embodiments of the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0040] In the description of the embodiments of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments of the present invention can be understood according to specific circumstances.

[0041] In the embodiments of the present invention, unless otherwise clearly specified and limited, the first feature being "above" or "below" the second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "above" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0042] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiment of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0043] like Figure 1 , Figure 3 , Figure 4 and Figure 5 As shown, the cell sorting device provided by the embodiment of the present invention includes a liquid storage barrel 300, a straw assembly 500 and a pressing assembly 400. The straw assembly 500 includes a straw 510 and a plug 520. The liquid storage barrel 300 is provided with a first through hole 310. The plug 520 is arranged at the first through hole 310. The plug 520 is constructed with a second through hole 521 extending along the axial direction of the liquid storage barrel 300. The straw 510 passes through the second through hole 521 and enters the liquid storage barrel 300. The plug 520 includes a telescopic section 522. One end of the telescopic section 522 is connected to the liquid storage barrel 300, and the other end is fixedly connected to the straw 510. The pressing assembly 400 can drive the telescopic section 522 to telescope along the axial direction of the liquid storage barrel 300 to drive the straw 510 to move.

[0044] In the cell sorting device of the embodiment of the present invention, the liquid storage barrel 300 is placed vertically, and the liquid storage barrel 300 is filled with a centrifugally layered cell solution to be sorted. The top of the liquid storage barrel 300 is provided with a first through hole 310, and a plug body 520 is installed at the first through hole 310. The plug body 520 is constructed with a second through hole 521 that is connected to the interior of the liquid storage barrel 300. The plug body 520 has a telescopic section 522. The telescopic section 522 can be compressed or extended when subjected to pressure, and can be extended or contracted to return to its original state when the pressure disappears. The fixed end of the telescopic section 522 is fixed on the liquid storage barrel 300, and the straw 510 passes through the second through hole 521 and is fixed to the movable end of the telescopic section 522. The pressing component 400 drives the movable end of the telescopic section 522 to move, so that the telescopic section 522 is compressed or extended as a whole, thereby driving the straw 510 to move downward along the axial direction of the liquid storage barrel 300. The pressing component 400 presses the telescopic section 522 to different degrees, so that the end of the straw 510 can reach the cell solution of different layers in the liquid storage barrel 300. By controlling the vertical telescopic distance of the telescopic section 522, the moving distance of the straw 510 is controlled to perform PBMC sorting. After the PBMC sorting is completed, the driving force of the pressing component 400 on the telescopic section 522 gradually disappears. While the telescopic section 522 is extended or contracted, it drives the straw 510 to move upward along the axial direction of the liquid storage barrel 300, and the straw 510 returns to its initial position under the action of the telescopic section 522. In this way, a complete cell PBMC sorting process is completed, and the PBMC sorting can be repeated multiple times. Through the cooperation of the pressing component 400 and the straw component 500, the manual sorting of the prior art is replaced, the sorting efficiency and accuracy are improved, and at the same time, the contamination of the cell solution by the outside world during the sorting process is avoided.

[0045] In one embodiment, the telescopic section 522 may be located outside the liquid storage barrel 300, that is, the straw 510 first passes through the second through hole 521 in the telescopic section 522 from the outside, then passes through the second through hole 521 in the plug body 520 located inside the liquid storage barrel 300, and finally enters the liquid storage barrel 300, while the straw 510 is fixed to the telescopic section 522. The pressing assembly 400 moves downward to press the telescopic section 522, and the telescopic section 522 compresses and drives the straw 510 to move downward along the axial direction of the liquid storage barrel 300. After the PBMC sorting is completed, the pressing assembly 400 moves upward and gradually leaves the telescopic section 522, and the telescopic section 522 stretches and recovers while driving the straw 510 to move upward along the axial direction of the liquid storage barrel 300.

[0046] In another embodiment, the telescopic section 522 may also be located outside the liquid storage barrel 300, that is, the straw 510 first passes through the second through hole 521 portion in the plug body 520 located outside the liquid storage barrel 300, then passes through the second through hole 521 portion in the telescopic section 522, and finally enters the liquid storage barrel 300, while the straw 510 is fixed to the telescopic section 522. The pressing assembly 400 moves downward to press the telescopic section 522, and the telescopic section 522 stretches and drives the straw 510 to move downward along the axial direction of the liquid storage barrel 300. After the PBMC sorting is completed, the pressing assembly 400 moves upward and gradually leaves the telescopic section 522. The telescopic section 522 shrinks and recovers while driving the straw 510 to move upward along the axial direction of the liquid storage barrel 300.

[0047] According to an embodiment provided by the present invention, the plug body 520 further includes a fixed section 523, and the fixed section 523 is coaxially arranged with the telescopic section 522. In this embodiment, the axial direction of the plug body 520 as a whole is the same as the axial direction of the liquid storage barrel 300, and the telescopic section 522 and the fixed section 523 can be a segmented plug body 520, or a nested plug body 520. In the case of the segmented type, when the telescopic section 522 is located outside the liquid storage barrel 300, the plug body 520 is sequentially a telescopic section 522 and a fixed section 523 from outside to inside along the axial direction of the liquid storage barrel 300, and the pressing component 400 directly presses the telescopic section 522 to drive the straw 510 to move; when the telescopic section 522 is located inside the liquid storage barrel 300, the plug body 520 is sequentially a telescopic section 522 and a fixed section 523 from inside to outside along the axial direction of the liquid storage barrel 300, and the pressing component 400 directly presses the fixed section 523 to drive the straw 510 to move, thereby driving the telescopic section 522 to expand and contract. In the nested case, the telescopic section 522 can be mounted on the outside of the fixed section 523 located outside the liquid storage barrel 300, or can be mounted on the outside of the fixed section 523 located inside the liquid storage barrel 300. The pressing component 400 directly presses the fixed section 523 to drive the straw 510 to move, thereby driving the telescopic section 522 to expand and contract.

[0048] The telescopic section 522 and the fixed section 523 are connected as one body, and the second through hole 521 constructed together inside the two can be a through hole of equal diameter or a through hole of variable diameter, so as to ensure smooth passage of the straw 510. The fixed section 523 can stabilize the straw 510, ensure the verticality of the straw 510, and guide the movement of the straw 510.

[0049] According to an embodiment provided by the present invention, the telescopic section 522 includes a telescopic member 5221 and a first end plate 5222, one end of the telescopic member 5221 abuts against the liquid storage barrel 300, and the other end is connected to the first end plate 5222, and the straw 510 passes through the first end plate 5222 and is fixedly connected to the first end plate 5222. In this embodiment, the telescopic member 5221 can select a fully enclosed retractable tubular structure, such as a bellows, a plastic tube, a spring, etc., or a fully enclosed retractable connecting rod structure. When the telescopic section 522 is located outside the liquid storage barrel 300, the upper end of the telescopic member 5221 is fixed to the first end plate 5222, and the lower end is abutted against the liquid storage barrel 300. When the telescopic section 522 is located inside the liquid storage barrel 300, the lower end of the telescopic member 5221 is fixed to the first end plate 5222, and the upper end is fixedly connected to the liquid storage barrel 300. The first end plate 5222 is provided with a mounting hole, and the mounting hole, the hollow part of the tubular structure or the connecting rod structure and the through hole inside the fixing section 523 together constitute the second through hole 521 of the plug body 520, and the straw 510 is fixed at the position of the mounting hole.

[0050] In this embodiment, the telescopic section 522 is located outside the liquid storage barrel 300, and the pressing assembly 400 moves downward to press the first end plate 5222, and the first end plate 5222 moves downward to drive the straw 510 to move downward, and the tubular structure or the connecting rod structure is compressed; the pressing assembly 400 moves upward and gradually leaves the first end plate 5222, and the tubular structure or the connecting rod structure is extended and restored, so that the first end plate 5222 moves upward and drives the straw 510 to move upward and return to its original position. In other embodiments, the telescopic section 522 is located inside the liquid storage barrel 300, and the pressing assembly 400 moves downward to press the fixed section 523, and the straw 510 moves downward to drive the first end plate 5222 to move downward, so that the tubular structure or the connecting rod structure is extended; the pressing assembly 400 moves upward and gradually leaves the fixed section 523, and the tubular structure or the connecting rod structure is contracted and restored, so that the first end plate 5222 moves upward and drives the straw 510 to move upward and return to its original position.

[0051] According to an embodiment provided by the present invention, the telescopic member 5221 is sleeved on the outside of the fixed section 523 , and the fixed section 523 is connected to the first end plate 5222 . In this embodiment, the fixed section 523 and the telescopic section 522 are nested to form the plug body 520, and the telescopic member 5221 adopts a spring, which is sleeved on the outside of the fixed section 523, and one end of the fixed section 523 is fixed to the first end plate 5222. In this embodiment, the telescopic section 522 is located outside the liquid storage barrel 300, and the lower end of the spring directly abuts against the top of the liquid storage barrel 300. When the pressing component 400 presses down the first end plate 5222, the spring is compressed, and the first end plate 5222, the fixed section 523 and the straw 510 are integrated and move downward synchronously; when the pressing component 400 moves upward and gradually leaves the first end plate 5222, the spring rebounds and stretches, and the restoring force acts on the first end plate 5222, and the first end plate 5222, the fixed section 523 and the straw 510 are integrated and move upward synchronously. In other embodiments, the telescopic section 522 is located inside the liquid storage barrel 300, and the upper end of the spring directly abuts against the top of the liquid storage barrel 300. When the pressing assembly 400 presses down the fixed section 523, the spring stretches, and the first end plate 5222, the fixed section 523 and the straw 510 are integrated and move downward synchronously; when the pressing assembly 400 moves upward and gradually leaves the fixed section 523, the spring rebounds and contracts, and the restoring force acts on the first end plate 5222. The first end plate 5222, the fixed section 523 and the straw 510 are integrated and move upward synchronously.

[0052] like Figure 2 As shown, according to an embodiment provided by the present invention, a sealing ring 530 is provided between the fixed section 523 and the first through hole 310. In the case where the fixed section 523 and the telescopic section 522 are nested and move synchronously, it is necessary to ensure good dynamic sealing between the first through hole 310 and the fixed section 523. Therefore, a sealing ring 530 is provided between the hole wall of the first through hole 310 and the outer wall of the fixed section 523 to achieve sealing, so that when the straw 510 and the plug body 520 move vertically downward, it is ensured that the gas and liquid in the container are in a completely sealed state and are not affected by the external environment. In other embodiments, the sealing structure between the fixed section 523 and the first through hole 310 is not limited to the sealing ring 530.

[0053] According to an embodiment provided by the present invention, the telescopic section 522 includes an annular second end plate 5224, the inner side of the second end plate 5224 is connected to the fixed section 523, one end surface of the second end plate 5224 is connected to the telescopic member 5221, and the other end surface is in contact with the liquid storage barrel 300. Figure 6 As shown, the telescopic section 522 and the fixed section 523 are segmented to form the plug body 520, and the telescopic member 5221 is a bellows, which is a fully sealed flexible structure that can be compressed or extended, and can also restore to an uncompressed or unextended state.

[0054] In this embodiment, the bellows is located outside the liquid storage barrel 300, the first end plate 5222 is fixed to the upper end of the bellows, and the second end plate 5224 is arranged at the lower end of the bellows. The second end plate 5224 is annular, the bottom surface of the second end plate 5224 contacts the liquid storage barrel 300, the top surface is connected to the bellows, and the inner side thereof is connected to the fixed section 523. The fixed section 523 and the second end plate 5224 can be an integrally formed structure, that is, the second end plate 5224 is a flange extending from the first through hole 310 of the fixed section 523 to the outside of the liquid storage barrel 300. The mutual cooperation of the first end plate 5222, the telescopic member 5221, the second end plate 5224 and the fixed section 523 can ensure that the telescopic member 5221 telescopes to drive the straw 510 to move, while ensuring a good sealing effect between the plug body 520 and the first through hole 310. When the pressing assembly 400 presses down the first end plate 5222, the bellows is compressed, the fixed section 523 is stationary, and the first end plate 5222 and the straw 510 move downward synchronously; when the pressing assembly 400 moves upward and gradually leaves the first end plate 5222, the bellows rebounds and stretches, and the restoring force acts on the first end plate 5222, and the first end plate 5222 and the straw 510 move upward synchronously.

[0055] In another embodiment, the bellows is located inside the liquid storage barrel 300, the first end plate 5222 is fixed to the lower end of the bellows, and the second end plate 5224 is arranged at the upper end of the bellows. The second end plate 5224 is annular, the top surface of the second end plate 5224 contacts the liquid storage barrel 300, the bottom surface is connected to the bellows, and the inner side thereof is connected to the fixed section 523. The fixed section 523 and the second end plate 5224 can be an integrally formed structure, that is, the second end plate 5224 extends from the outside of the liquid storage barrel 300 to the flange of the first through hole 310 as the fixed section 523. The mutual cooperation of the first end plate 5222, the telescopic member 5221, the second end plate 5224 and the fixed section 523 can ensure that the telescopic member 5221 telescopes and drives the straw 510 to move, while ensuring a good sealing effect between the plug body 520 and the first through hole 310. When the pressing assembly 400 presses down the straw 510, the bellows stretches, the fixed section 523 remains stationary, and the first end plate 5222 and the straw 510 move downward synchronously; when the pressing assembly 400 moves upward and gradually leaves the straw 510, the bellows rebounds and contracts, and the restoring force acts on the first end plate 5222, so that the first end plate 5222 and the straw 510 move upward synchronously.

[0056] like Figure 7 and Figure 8As shown, in one embodiment, the telescopic member 5221 may also be a connecting rod, the upper end of the connecting rod is fixed with the first end plate 5222, the lower end of the connecting rod is provided with a second end plate 5224, the bottom surface of the second end plate 5224 contacts the liquid storage barrel 300, the top surface is connected to the beam, and the inner side thereof is connected to the fixed section 523. When the pressing assembly 400 presses down the first end plate 5222, the connecting rod is folded and compressed, the fixed section 523 is stationary, and the first end plate 5222 and the straw 510 move downward synchronously; when the pressing assembly 400 moves upward and gradually leaves the first end plate 5222, the connecting rod is extended, the restoring force acts on the first end plate 5222, and the first end plate 5222 and the straw 510 move upward synchronously. The liquid storage barrel 300 is divided into a first liquid layer 360, a second liquid layer 370 and a third liquid layer 380 from top to bottom. As the pressing assembly 400 moves downward, the nozzle of the straw 510 can reach the first liquid layer 360, the second liquid layer 370 and the third liquid layer 380 in turn, and the three liquid layers can be sorted respectively. In other embodiments, the liquid layers from top to bottom in the liquid storage barrel 300 can be more than three layers or two layers.

[0057] According to an embodiment provided by the present invention, the straw assembly 500 further includes a third end plate 540 for contacting with the pressing assembly 400, and the third end plate 540 is arranged on the plug body 520, and there is a gap between the plug body 520 and the third end plate 540 for the straw 510 to pass through. In this embodiment, the third end plate 540 is further arranged above the plug body 520, and when the telescopic section 522 is located outside the liquid storage barrel 300, the third end plate 540 is coaxially arranged with the first end plate 5222, and connected by a connecting rod 550, and the length of the connecting rod 550 forms a certain gap between the first end plate 5222 and the second end plate 5224, and the straw 510 enters the mounting hole of the first end plate 5222 through the gap, and the pressing assembly 400 contacts the third end plate 540 and presses, thereby realizing the pressing of the telescopic section 522, which can effectively prevent the pressing assembly 400 from contacting the straw 510, causing squeezing of the straw 510 to affect the subsequent sorting work. When the telescopic section 522 is located inside the liquid storage barrel 300, the third end plate 540 is coaxially arranged with the fixed section 523 and connected by a connecting rod 550. The length of the connecting rod 550 forms a certain gap between the upper end surface of the fixed section 523 and the second end plate 5224. The straw 510 passes through the gap and enters the second through hole 521 of the fixed section 523. The pressing component 400 contacts and presses the third end plate 540 to press the telescopic section 522, which can effectively prevent the pressing component 400 from contacting the straw 510 and squeezing the straw 510 to affect subsequent sorting work.

[0058] In one embodiment, the pressing assembly 400 may include a pressing plate 410, a pressing rod 420 and a driver, wherein the pressing plate 410 is horizontally arranged, and the driver is connected to the pressing plate 410 via the pressing rod 420, and the driver drives the pressing plate 410 to move up and down via the pressing rod 420 to act on the third end plate 540. The driver may include components such as a cylinder.

[0059] According to an embodiment provided by the present invention, the cell sorting device of the embodiment of the present invention also includes a support base 200 and a driving member. The support base 200 is horizontally arranged, and the liquid storage barrel 300 is rotatably connected to the support base 200 through a rotating shaft. The rotating shaft is horizontally arranged and perpendicular to the axial direction of the liquid storage barrel 300. The driving member is connected to the support base 200 to drive the support base 200 to rotate around a rotating axis perpendicular to the axial direction of the rotating shaft.

[0060] The cell sorting device of the embodiment of the present invention can also achieve the effect of centrifugal stratification of the cell solution. Corresponding mounting holes are set on the support seat 200 and the liquid storage barrel 300. One end of the rotating shaft is inserted into the mounting hole of the support seat 200, and the other end is inserted into the mounting hole of the liquid storage barrel 300, so that the liquid storage barrel 300 is rotatably connected to the support seat 200. The support seat 200 is arranged horizontally. When the support seat 200 is in a stationary state, the liquid storage barrel 300 is placed vertically due to gravity, that is, the axial direction of the liquid storage barrel 300 is perpendicular to the horizontal direction of the support seat 200, and the axial direction of the rotating shaft is parallel to the horizontal direction of the support seat 200; the driving member drives the support seat 200 to rotate in the horizontal direction. During the rotation process, the liquid storage barrel 300 gradually rotates from a vertical state to a horizontal state due to the effect of inertia, and the cell solution in the liquid storage barrel 300 begins to be centrifugally layered under the action of centrifugal force, and the required cells are in the outermost layer, and the impurities / culture medium are in the inner layer, that is, the cell solution is layered along the axial direction of the liquid storage barrel 300, and the cells are at the bottom of the barrel of the liquid storage barrel 300; after the centrifugation is completed, the driving member drives the rotation speed of the support seat 200 to slowly decrease, and the liquid storage barrel 300 also slowly rotates from a horizontal state to a vertical state to reset, at which time the layering of the cell solution can still be maintained along the axial direction of the liquid storage barrel 300, and the required cells are in the lower layer, and the impurities / culture medium are in the upper layer, that is, the cells are at the bottom of the barrel of the liquid storage barrel 300. After the liquid storage barrel 300 is rotated to a vertical state, the cells and the impurities / culture medium are kept in layers, and the impurities / culture medium or cells are discharged from the liquid storage barrel 300 to achieve concentration.

[0061] The cell sorting device of the present invention drives the support seat 200 to rotate through the driving member, and the liquid storage barrel 300 is turned from a vertical state to a horizontal state, and the cell solution in the liquid storage barrel 300 is centrifugally layered, which can ensure effective and accurate centrifugal layering. By controlling the angular acceleration of the driving member movement, it is ensured that the liquid storage barrel 300 is turned from a horizontal state to a vertical state. The cells and impurities / culture medium are not mixed together, and the cells are always at the bottom of the barrel of the liquid storage barrel 300 until the liquid storage barrel 300 is turned to a vertical state. The cell solution is layered and static in the liquid storage barrel 300, and then the cell solution in the liquid storage barrel 300 is concentrated to achieve the concentration of the cell solution in a static state, without the need to perform cell concentration and separation in a centrifugal state, reducing cell damage, simplifying the device structure, and simplifying the concentration operation. The liquid storage barrel 300 can be compatible with cell concentrations of various sizes, and the volume of the concentrated cell solution can reach more than 2L. The present invention realizes fully enclosed and fully automated operation, and the volume that can be concentrated compared to the prior art is increased by more than 3 times, and the viability of the cells after concentration is increased by more than 10%.

[0062] In this embodiment, the driving member can be a motor, and the output shaft of the motor is vertically connected to the support base 200, and the connection position is the rotation center of the support base 200. The motor can also be connected to a speed regulator of an adapted model to meet the needs of centrifugation. In other embodiments, the driving member can also adopt other device structures that can drive the support base 200 to rotate, which is not limited by this embodiment.

[0063] In this embodiment, a first through hole 310 is provided on the top of the liquid storage barrel 300, and a pipette 510 is inserted into the liquid storage barrel 300 from the outside through the first through hole 310. Cell solution can be injected into the liquid storage barrel 300 through the pipette 510, and cells or impurities / cultures in the liquid storage barrel 300 can also be sucked out. The pipettes 510 of each liquid storage barrel 300 can be controlled individually or uniformly, which is convenient for concentration operation. After ensuring the sealing between the liquid storage barrel 300 itself and the first through hole 310 of the liquid storage barrel 300 and the pipette 510, centrifugal stratification, sorting or concentration of the cell solution in a fully enclosed environment can be achieved. During the centrifugal stratification, sorting or concentration process, the cell solution is in the liquid storage barrel 300 to avoid being contaminated by the external environment.

[0064] To ensure that the rotation of the liquid storage barrel 300 is not affected, the straw 510 should be made of a deformable material that can adapt to the rotation process of the liquid storage barrel 300, such as a soft plastic tube, a rubber tube, etc. When the liquid storage barrel 300 changes between a horizontal state and a vertical state, the straw 510 can be deformed and bent to adapt to the position state of the liquid storage barrel 300.

[0065] According to an embodiment provided by the present invention, the support seat 200 is provided with a liquid-passing tube 210, and the suction tube 510 is connected to the liquid-passing tube 210. In this embodiment, the liquid-passing tube 210 is constructed on the upper surface of the support seat 200 and is integrally formed with the support seat 200, that is, the axial direction of the liquid-passing tube 210 is perpendicular to the horizontal direction of the support seat 200. When the support seat 200 is in a stationary state, the liquid-passing tube 210 is vertically upward, and the part of the suction tube 510 located outside the liquid storage barrel 300 is connected to the lower part of the liquid-passing tube 210. When the driving member drives the support seat 200 to rotate, the liquid-passing tube 210 rotates synchronously with the suction tube 510. Cell solution can be injected through the upper port of the liquid-passing tube 210, and cells or impurities / culture matrix can also be discharged.

[0066] like Fig. 9 As shown, according to an embodiment provided by the present invention, the liquid-through pipe 210 is coaxially arranged with the support seat 200, and the upper end of the liquid-through pipe 210 is communicated with the pipeline 600 through the connecting sleeve 230, and a bearing 240 for connecting with the pipeline 600 is arranged in the connecting sleeve 230, and a sealing ring 250 is arranged between the pipeline 600 and the inner wall of the connecting sleeve 230. In this embodiment, only one liquid-through pipe 210 is arranged on the support seat 200, and the axial direction of the liquid-through pipe 210 is the same as the rotational axial direction of the driving member driving the support seat 200, and each suction pipe 510 is communicated with the lower end of the liquid-through pipe 210. In other embodiments, a plurality of liquid-through pipes 210 may also be arranged on the support seat 200 to cooperate with the suction pipe 510, and each liquid-through pipe 210 corresponds to one suction pipe 510, and the number of liquid-through pipes 210 is selected according to actual needs.

[0067] In this embodiment, the lower end of the liquid pipe 210 is integrally formed with the support seat 200, and one end of the connecting sleeve 230 is sleeved on the upper end. The other end of the connecting sleeve 230 is provided with a bearing 240, and the bearing 240 is connected to the pipeline 600 of the liquid supply device. The liquid of the liquid supply device is sent into the connecting sleeve 230 through the pipeline 600, and then enters the liquid pipe 210 from the connecting sleeve 230. When the driving member drives the support seat 200 to rotate, the liquid pipe 210 rotates along its axial direction, driving the connecting sleeve 230 to rotate. The connecting sleeve 230 is rotatably connected to the pipeline 600 of the liquid supply device through the bearing 240, so that the pipeline 600 of the liquid supply device remains stationary, which not only ensures that the liquid passage is unobstructed, but also ensures that the external liquid supply device does not affect the centrifugal operation process. A sealing ring 250 is provided between the part of the pipeline 600 entering the connecting sleeve 230 and the connecting sleeve 230, forming a dynamic sealing structure at the position where the pipeline 600 is connected to the liquid pipe 210, ensuring that the transported liquid does not flow back and does not overflow the connecting sleeve 230. In other embodiments, the connection between the pipeline 600 and the liquid passage 210 can be connected by a dynamic sealing structure of different forms, as long as the dynamic sealing can be well ensured. The number and relative position of the sealing ring 250 and the bearing 240 can also be selected according to the actual structural requirements and are not limited by this embodiment.

[0068] According to an embodiment provided by the present invention, the liquid storage barrel 300 is provided with a third through hole 330 on the upper part of the barrel wall on one side away from the rotation axis of the support seat 200, and the third through hole 330 is connected to the inside of the liquid storage barrel 300, and a breathable membrane 340 is provided at the third through hole 330. In this embodiment, during the centrifugation process, negative pressure will be generated in the liquid storage barrel 300, and smooth liquid circulation cannot be guaranteed. Therefore, the third through hole 330 is opened on the liquid storage barrel 300, and a breathable membrane 340 is provided to seal the third through hole 330. The breathable membrane 340 is connected to the outside atmosphere to ensure that the pressure values ​​inside and outside the liquid storage barrel 300 are the same, and avoid a large pressure difference that causes the inability to inject and discharge samples. During centrifugal rotation, the liquid storage barrel 300 rotates, and the barrel wall on the side of the rotation axis away from the support seat 200 will be facing upward. The solution in the liquid storage barrel 300 at the location of the third through hole 330 will not contact the breathable membrane 340. In a static state, the solution in the liquid storage barrel 300 will not reach the third through hole 330 to contact the breathable membrane 340, ensuring that the breathable membrane 340 will not fail when exposed to water.

[0069] According to an embodiment provided by the present invention, the support seat 200 is configured with a groove 220 that is recessed from the edge toward the rotation axis of the support seat 200, and the liquid storage barrel 300 is disposed in the groove 220. In this embodiment, the edge of the support seat 200 is recessed toward the middle of the support seat 200 to form the groove 220, and the liquid storage barrel 300 is embedded in the groove 220. Mounting holes are provided on the opposite side walls of the groove 220, corresponding to the installation of two rotating shafts connecting the liquid storage barrel 300. The arrangement of the groove 220 can not only ensure that the support seat 200 and the liquid storage barrel 300 rotate synchronously for centrifugal concentration, but also reduce the space occupied by the device and make the device structure compact.

[0070] In one embodiment, there are multiple liquid storage barrels 300, and the multiple liquid storage barrels 300 are evenly distributed around the rotation axis of the support base 200. In this embodiment, there are four liquid storage barrels 300, and four grooves 220 and four straws 510 are correspondingly provided, and the four liquid storage barrels 300 are evenly distributed around a liquid passage 210. When the driving member drives the support base 200 to rotate, the force balance of the device can be ensured, and the centrifugal force on each liquid storage barrel 300 is equal and balanced. In other embodiments, the number of liquid storage barrels 300 can be selected according to actual needs and is not limited by this embodiment.

[0071] like Fig.10 As shown, the cell sorting and concentration system provided by the embodiment of the present invention includes a control device 700, a liquid supply device and the cell sorting device 100 of the above embodiment, the liquid supply device and the driving member are connected to the control device 700, the liquid supply device includes a bracket 800 and a liquid channel 900, the bracket 800 is used to place the liquid bag, and the liquid channel 900 is connected to the liquid storage barrel 300.

[0072] In the cell sorting and concentration system of the embodiment of the present invention, the control device 700 has a software operation interface, which can perform human-computer interaction and control the start and stop and operation of the equipment. It is connected to the driving part of the cell sorting device 100 and can control the rotation speed and acceleration of the driving part, as well as the start and stop time of the driving part; the bracket 800 can hang liquid bags containing blood, buffer solution, separation liquid, etc.; the liquid path channel 900 transports the blood, buffer solution, separation liquid, etc. placed on the bracket 800 to the liquid storage barrel 300 in steps. After debugging the operating program in the control device 700, the parameters are input into the control device 700 to automatically control the blood and other liquids in the bracket 800 to flow into the liquid storage barrel 300 of the cell sorting device 100 through the liquid channel 900, and then control the rotation of the driving member to control the switching of the liquid storage barrel 300 between the horizontal and vertical states, so that the cell solution is centrifugally stratified when the liquid storage barrel 300 is in the horizontal state, and statically sorted or concentrated when the liquid storage barrel 300 is in the vertical state, thereby realizing fully enclosed and fully automated cell sorting or concentration, replacing the manual sorting or concentration in the prior art, avoiding the influence of the external environment, and improving the sorting or concentration efficiency and accuracy.

[0073] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A cell sorting device, characterized in that: The invention comprises a liquid storage barrel, a straw assembly and a pressing assembly, wherein the straw assembly comprises a straw and a plug body, the liquid storage barrel is provided with a first through hole, the plug body is arranged at the first through hole, the plug body is structured with a second through hole extending along the axial direction of the liquid storage barrel, the straw passes through the second through hole and enters the liquid storage barrel, the plug body comprises a telescopic section, one end of the telescopic section is connected to the liquid storage barrel, and the other end is fixedly connected to the straw, the pressing assembly can drive the telescopic section to telescope along the axial direction of the liquid storage barrel to drive the straw to move; the plug body also comprises a fixed section, the fixed section is coaxially arranged with the telescopic section; the telescopic section comprises a telescopic member and a first end plate, one end of the telescopic member is connected to the first end plate, and the other end of the telescopic member is fixedly connected to the first end plate. The first end plate is connected to the liquid storage barrel, and the other end is connected to the first end plate, the straw passes through the first end plate and is fixedly connected to the first end plate; the telescopic piece is sleeved on the outside of the fixed section, and the fixed section is connected to the first end plate; a sealing ring is provided between the fixed section and the first through hole; the telescopic section includes an annular second end plate, the inner side of the second end plate is connected to the fixed section, one end face of the second end plate is connected to the telescopic piece, and the other end face is abutted against the liquid storage barrel; the straw assembly also includes a third end plate for contacting the pressing assembly, the third end plate is provided on the plug body, and there is a gap between the plug body and the third end plate for the straw to pass through.

2. The cell sorting device according to claim 1, characterized in that: It also includes a support base and a driving member, the support base is horizontally arranged, the liquid storage barrel is rotatably connected to the support base via a rotating shaft, the rotating shaft is horizontally arranged and perpendicular to the axial direction of the liquid storage barrel, and the driving member is connected to the support base to drive the support base to rotate around a rotating axis perpendicular to the axial direction of the rotating shaft.

3. The cell sorting device according to claim 2, characterized in that: The support seat is provided with a liquid passage pipe, and the suction pipe is communicated with the liquid passage pipe.

4. The cell sorting device according to claim 3, characterized in that: The liquid passage pipe is coaxially arranged with the support seat, the upper end of the liquid passage pipe is connected with the pipeline through a connecting sleeve, a bearing for connecting with the pipeline is arranged in the connecting sleeve, and a sealing ring is arranged between the pipeline and the inner wall of the connecting sleeve.

5. The cell sorting device according to claim 2, characterized in that: The liquid storage barrel is provided with a third through hole at the upper part of the barrel wall on one side away from the rotation axis of the support seat, the third through hole is communicated with the interior of the liquid storage barrel, and a breathable membrane is provided at the third through hole.

6. The cell sorting device according to claim 2, characterized in that: The support seat is configured with a groove which is recessed from the edge toward the rotation axis of the support seat, and the liquid storage barrel is arranged in the groove.

7. A cell sorting and concentration system, characterized in that: It comprises a control device, a liquid supply device and the cell sorting device according to any one of claims 1 to 6, wherein the liquid supply device and the driving member are both connected to the control device, the liquid supply device comprises a bracket and a liquid channel, the bracket is used to place a liquid bag, and the liquid channel is connected to the liquid storage barrel.

Images