An enzymatic hydrolysis device and method controlled by a cluster of micro enzyme-carrying particles

Abstract

The present invention relates to an enzymatic hydrolysis device controlled by a cluster of micro enzyme-carrying particles, which includes a tank body provided with a feed inlet. Paired Z-direction excitation coils are respectively arranged at the top and bottom of the tank body. Paired annular excitation coils are respectively arranged on the left and right outer circumferences of the tank body. A top motor is also arranged at the top of the tank body. A stirrer is arranged inside the tank body. The top motor drives the stirrer to rotate. A mixed solution is contained inside the tank body. The mixed solution contains biomass materials to be enzymatically hydrolyzed and a plurality of micro enzyme-carrying particles. The stirrer includes a stirring shaft and a plurality of paddle-shaped stirring blades arranged on the stirring shaft. The present invention also discloses a method for performing enzymatic hydrolysis using this enzymatic hydrolysis device. In the present invention, the biomass materials to be enzymatically hydrolyzed in the tank body are fully contacted with the enzyme hydrolysis agent from the beginning of entry, realizing the rapid enzymatic hydrolysis of the biomass materials.

Description

Technical Field

[0001] The present invention belongs to the technical field of biomass enzymatic hydrolysis equipment, and relates to an enzymatic hydrolysis device and method controlled by a cluster of micro enzyme-carrying particles. Background Art

[0002] A bioreactor is a device that obtains target metabolite medicinal proteins by culturing cells in vitro by simulating the cell survival environment. According to the requirements of different working conditions, the stirring tank will also present different structures. The common one is generally a cylindrical tank body. Due to the special structure of the cylindrical tank body, the internal fluid can be made more regular during the rotational flow process, reducing the generation of wall dead zones. The stirrer is an important part of the bioreactor. It stirs to generate a mixed flow field, enabling the rapid transfer of nutrients in the reactor and serving as the power source for driving cell growth. However, the shear force generated by the impeller during stirring will damage the cells. Therefore, how to find a suitable reactor type and structural parameters is the main problem in the design of the bioreactor impeller. If the stirrer is classified according to the macroscopic flow pattern generated in the stirring container, the stirrer can be divided into two categories: axial flow type and radial flow type. Radial flow type: The fluid generates a radial flow under the stirring of the impeller. After colliding with the wall, two circulations (upward and downward respectively) are generated, and finally both return to the impeller. Axial flow type: The fluid near the impeller flows out axially, collides with the bottom of the stirring tank, then turns and flows upward along the wall from bottom to top, and finally completes a cycle along the axis downward. Its entire flow field is a relatively simple "single cycle" motion pattern. For the mixed slurry to be stirred in the bioreactor, the stirring viscosity of different media refers to the impedance ability of the fluid to flow. Its definition is: when the liquid flows at a speed of 1 cm / s, the magnitude of the shear force required on each square centimeter plane is the dynamic viscosity, with the unit of Pa·s. Viscosity is a property of the fluid. When the fluid flows in a pipeline, there are three states: laminar flow, transitional flow, and turbulent flow. At the same time, the fluid type is also divided into Newtonian fluid, non-Newtonian fluid, etc.

[0003] The bioreactor is used for high-concentration saccharification enzymatic hydrolysis fermentation of materials such as corncobs and straws. The material is a pretreatment slurry of one or more mixtures of agricultural and forestry residues such as corncob residues, corn straws, and wheat straws, with a dry matter content of 20% - 25%, or a higher solid content. For this kind of high-viscosity biological slurry, a combined stirrer is widely used, including a stirring shaft. An inclined paddle stirrer is arranged on the upper layer of the stirring shaft, and a disk stirrer is arranged on the lower layer. However, the stirring effect is not ideal. Through numerical simulation, it is found that the circulating flow is obvious, and the stirring speed of the slurry around the stirring shaft is relatively small, resulting in a slow mixing speed of the slurry in the bioreactor and a slow decrease in viscosity.

[0004] On the other hand, in existing bioreactors, the biomass materials to be enzymatically hydrolyzed and the enzymatic hydrolyzing agents are fed in batches. Some of the biomass materials cannot come into sufficient contact with the enzymatic hydrolyzing agents, resulting in insufficient enzymatic hydrolysis or inability to be enzymatically hydrolyzed, wasting raw materials and increasing production costs. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide an enzymatic hydrolysis device and method controlled by a cluster of micro enzyme-carrying particles, enabling the biomass materials to be enzymatically hydrolyzed to come into sufficient contact with the enzymatic hydrolyzing agents from the moment they enter the tank, and achieving rapid enzymatic hydrolysis of the biomass materials.

[0006] The present invention is realized as follows. It provides an enzymatic hydrolysis device controlled by a cluster of micro enzyme-carrying particles, including a tank with a feed inlet. Pairwise Z-direction exciting coils are respectively arranged at the top and bottom of the tank. Pairwise annular exciting coils are respectively arranged on the left and right outer peripheries of the tank. A top motor is also arranged at the top of the tank. A stirrer is arranged inside the tank. The top motor drives the stirrer to rotate. A mixed solution is contained inside the tank, and the mixed solution contains biomass materials to be enzymatically hydrolyzed and a plurality of micro enzyme-carrying particles. The stirrer includes a stirring shaft and a plurality of paddle-shaped stirring blades arranged on the stirring shaft.

[0007] Further, the plurality of paddle-shaped stirring blades are respectively arranged on the upper part of the stirring shaft, and a plurality of turbine-shaped stirring blades are also arranged on the lower part of the stirring shaft.

[0008] Further, at least one main paddle-shaped stirring blade is provided among the plurality of paddle-shaped stirring blades, and the length of the main paddle-shaped stirring blade is greater than that of the other paddle-shaped stirring blades.

[0009] Further, 2 to 7 paddle-shaped stirring blades are arranged on the stirring shaft.

[0010] Further, at least one main turbine-shaped stirring blade is provided among the plurality of turbine-shaped stirring blades, and its length is greater than that of the other turbine-shaped stirring blades.

[0011] Further, 2 to 8 turbine-shaped stirring blades are arranged on the stirring shaft.

[0012] Further, the micro enzyme-carrying particles include a magnetic response material and a deformation adsorption complex combined together.

[0013] The present invention is realized as follows. It provides an enzymatic hydrolysis method controlled by a cluster of micro enzyme-carrying particles, using the enzymatic hydrolysis device controlled by a cluster of micro enzyme-carrying particles as described above, including the following steps:

[0014] Step 1: Add multiple micro enzyme-carrying particles and water into the tank body. At the same time, control the voltage magnitude and period of the Z-direction excitation coil and the annular excitation coil, so that the magnetic field center is located in the feed port area of the tank body. Attract multiple micro particles dispersed in the tank body through the magnetic field and gather them at the feed port. The micro enzyme-carrying particles keep colliding under the action of the magnetic force, so that the enzyme solution inside the micro enzyme-carrying particles is continuously released.

[0015] Step 2: Then add the biomass material to be enzymatically hydrolyzed into the tank body. At the same time, turn on the stirrer, so that the biomass material to be enzymatically hydrolyzed can fully contact the enzyme solution from the moment it enters the tank body, realizing the rapid enzymatic hydrolysis of the biomass material.

[0016] Compared with the prior art, in the enzymatic hydrolysis device and method controlled by the micro enzyme-carrying particle cluster of the present invention, multiple micro particles are arranged in the biomass material solution to be enzymatically hydrolyzed in the tank body. The multiple micro particles gather at the feed port under the Z-direction excitation coil and the annular excitation coil outside the tank body, and keep colliding under the action of the magnetic force, so that the enzyme solution inside the micro enzyme-carrying particles is continuously released and fully mixed with the biomass material entering the tank body, so that the biomass material to be enzymatically hydrolyzed can fully contact the enzyme solution from the moment it enters the tank body, realizing the rapid enzymatic hydrolysis of the biomass material. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a three-dimensional schematic diagram of a preferred embodiment of the enzymatic hydrolysis device of the present invention;

[0018] Figure 2 is Figure 1 a perspective schematic diagram of the tank body in DETAILED DESCRIPTION OF THE INVENTION

[0019] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer, the present invention will be further described in detail below with reference to the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0020] Please refer to Figure 1 and Figure 2 as shown. A preferred embodiment of the enzymatic hydrolysis device controlled by the micro enzyme-carrying particle cluster of the present invention includes a tank body 1 provided with a feed port 6. Paired Z-direction excitation coils 2 are respectively arranged at the top and bottom of the tank body 1, and paired annular excitation coils 3 are respectively arranged on the left and right outer circumferences of the tank body 1. A top motor 4 is also arranged at the top of the tank body 1. A stirrer 5 is arranged inside the tank body 1, and the top motor 4 drives the stirrer 5 to rotate.

[0021] Inside the tank body 1, there is a mixed solution, which contains the biomass material to be enzymatically hydrolyzed and multiple micro enzyme-carrying particles (not shown in the figure). By regulating the voltages and periods of two pairs of Z-direction excitation coils 2 and the annular excitation coil 3, the micro enzyme-carrying particles are gathered at the feed inlet 6 of the tank body 1. The micro enzyme-carrying particles keep colliding under the magnetic force, so that the enzymatic hydrolysis agent liquid inside the micro enzyme-carrying particles is continuously released. The biomass material to be enzymatically hydrolyzed entering the tank body 1 comes into full contact with the enzymatic hydrolysis agent liquid from the very beginning, realizing the rapid enzymatic hydrolysis of the biomass material.

[0022] The stirrer 5 includes a stirring shaft 7 and a plurality of paddle-type stirring blades 8 arranged on the stirring shaft 7.

[0023] The plurality of paddle-type stirring blades 8 are respectively arranged on the upper part of the stirring shaft 7, and a plurality of turbine-type stirring blades 9 are also arranged on the lower part of the stirring shaft 7.

[0024] Among the plurality of paddle-type stirring blades 8, at least one main paddle-type stirring blade is provided, and the length of the main paddle-type stirring blade is greater than that of other paddle-type stirring blades.

[0025] There are 2 - 7 paddle-type stirring blades 8 arranged on the stirring shaft 7. In this embodiment, three paddle-type stirring blades 8 are provided. The working speed of the paddle-type stirring blades 8 is relatively high, and the circumferential speed of the outer edge of the blade is generally 5 m / s - 15 m / s. The paddle-type stirring blades 8 mainly cause axial liquid flow and a large circulation volume, and are suitable for stirring low-viscosity (<2 Pa·s) liquids, emulsions, and suspensions with a solid particle content of less than 10%. The stirring shaft 7 can also be inserted into the tank horizontally or obliquely. At this time, the circulation loop of the liquid flow is asymmetric, which can increase turbulence and prevent the liquid surface from sagging.

[0026] Among the plurality of turbine-type stirring blades 9, at least one main turbine-type stirring blade is provided, and its length is greater than that of other turbine-type stirring blades.

[0027] There are 2 - 8 turbine-type stirring blades 9 arranged on the stirring shaft 7. In this embodiment, six turbine-type stirring blades 9 are provided. The ratio of the outer diameter, width, and height of the turbine-type stirring blades 9 is generally 20:5:4, and the circumferential speed is generally 3 m / s - 8 m / s. When the stirring shaft 7 rotates, it causes a highly turbulent radial flow, which is suitable for the dispersion of gases and immiscible liquids and the liquid-liquid phase reaction process. The viscosity of the liquid to be stirred generally does not exceed 25 Pa·s.

[0028] The purpose of setting the main paddle-type stirring blade and the main turbine-type stirring blade is not only to drive the fully contacted biomass material to other areas inside the tank body 1 by virtue of their speeds, but also to apply a collision force and a squeezing force to the multiple micro enzyme-carrying particles, facilitating the release of the enzymatic hydrolysis agent liquid they carry.

[0029] The micro enzyme-carrying particles include a magnetic response material and a deformation adsorption complex combined together. Before use, the micro enzyme-carrying particles are placed in a high-concentration liquid enzyme preparation, and pressure is applied to fill the internal voids of the micro particles with the enzyme preparation. The micro enzyme-carrying particles can be reused.

[0030] The present invention also discloses an enzymatic hydrolysis method controlled by a cluster of micro enzyme-carrying particles. Using the enzymatic hydrolysis device controlled by a cluster of micro enzyme-carrying particles as described above, it includes the following steps:

[0031] Step 1: Add a plurality of micro enzyme-carrying particles and water into the tank body 1. At the same time, control the voltage magnitude and period of the Z-direction excitation coil 2 and the annular excitation coil 3, so that the magnetic field center is located in the feeding port 6 area of the tank body. Attract the plurality of micro particles dispersed in the tank body 1 over and gather them at the feeding port 6 through the magnetic field. The micro enzyme-carrying particles keep colliding under the action of the magnetic force, so that the enzyme solution inside the micro enzyme-carrying particles is continuously released.

[0032] Step 2: Then add the biomass material to be enzymatically hydrolyzed into the tank body 1. At the same time, turn on the stirrer 5, so that the biomass material to be enzymatically hydrolyzed can fully contact the enzyme solution from the moment it enters the tank body 1, realizing the rapid enzymatic hydrolysis of the biomass material. Adjust the magnetic force magnitude to control the amount of enzyme solution released by the plurality of micro enzyme-carrying particles to match the added biomass material.

[0033] The magnetic field control device of the Z-direction excitation coil 2 and the annular excitation coil 3 is a two-dimensional Helmholtz coil control system, including a power supply, a power supply circuit and electromagnetic coils. The power supply is the excitation source for providing the electromagnetic field device, and the power supply circuit distributes the voltage of the power supply to the electromagnetic coils. The electromagnetic coils include the Z-direction excitation coil 2 and the annular excitation coil 3. A silicon steel bar is fixed in the middle of the Z-direction excitation coil 2 and the annular excitation coil 3. The upper and lower positions and the left and right positions of the tank body 1 are divided into two groups of electromagnetic coil pairs. By controlling the voltage magnitude, on-off and period control of the Z-direction excitation coil 2 and the annular excitation coil 3, various magnetic fields are generated in the stirring area of the tank body 1, so as to drive the aggregation of the micro enzyme-carrying particles in the tank body 1.

[0034] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An enzymatic hydrolysis device controlled by a cluster of micro enzyme-carrying particles, comprising a tank body provided with a feed inlet, characterized in that, Pairs of Z - direction exciting coils are respectively arranged at the top and bottom of the tank body. Pairs of annular exciting coils are respectively arranged at the left and right outer peripheral edges of the tank body. A top motor is also arranged at the top of the tank body. A stirrer is arranged inside the tank body. The top motor drives the stirrer to rotate. A mixed solution is contained inside the tank body, and the mixed solution contains biomass materials to be enzymolyzed and a plurality of micro - enzyme - carrying particles; the stirrer includes a stirring shaft and a plurality of paddle - type stirring blades arranged on the stirring shaft; the plurality of paddle - type stirring blades are respectively arranged on the upper part of the stirring shaft, and a plurality of turbine - type stirring blades are also arranged on the lower part of the stirring shaft; At least one main paddle - type stirring blade is provided among the plurality of paddle - type stirring blades, and the length of the main paddle - type stirring blade is greater than the lengths of other paddle - type stirring blades; At least one main turbine - type stirring blade is provided among the plurality of turbine - type stirring blades, and its length is greater than that of other turbine - type stirring blades; The micro - enzyme - carrying particles include a magnetic - responsive material and a deformation - adsorption complex combined together.

2. The enzymatic hydrolysis device controlled by the micro enzyme-carrying particle clusters as described in claim 1, wherein There are 2 - 7 paddle - type stirring blades arranged on the stirring shaft.

3. The enzymatic hydrolysis device controlled by the micro enzyme-carrying particle clusters according to claim 1, wherein There are 2 - 8 turbine - type stirring blades arranged on the stirring shaft.

4. An enzymatic hydrolysis method controlled by a cluster of micro enzyme-carrying particles, characterized in that, Using the enzymolysis device controlled by a cluster of micro - enzyme - carrying particles as described in any one of claims 1 to 3, includes the following steps: Step 1: Add a plurality of micro - enzyme - carrying particles and water into the tank body. At the same time, control the voltage magnitude and period of the Z - direction exciting coils and the annular exciting coils so that the magnetic field center is located in the feed port area of the tank body. Attract and gather the plurality of micro - particles dispersed in the tank body to the feed port through the magnetic field. The micro - enzyme - carrying particles keep colliding under the magnetic force, so that the enzymolysis agent liquid inside the micro - enzyme - carrying particles is continuously released; Step 2: Then add the biomass materials to be enzymolyzed into the tank body. At the same time, start the stirrer so that the biomass materials to be enzymolyzed are fully in contact with the enzymolysis agent from the moment they enter the tank body, realizing the rapid enzymolysis of the biomass materials.

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