Triphenylmethyl light-emitting free radical material as well as preparation method and application thereof

Through the development of trityl-based luminescent free radical materials, multiple signal detection of biothiols, especially the accurate detection of cysteine, has been achieved, which solves the problem that fluorescent molecules in the existing technology cannot achieve multiple signal detection, and has High selectivity and sensitivity.

CN113603598AActive Publication Date: 2021-11-05JILIN UNIVERSITY
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Patent Information

Application Number
CN202111053658.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2021-09-09
Publication Date
2021-11-05
Estimated Expiration
2041-09-09

AI Technical Summary

Technical Problem

Existing fluorescent molecules are difficult to achieve multiple signal detection of biothiols, and cannot eliminate background fluorescence interference, making it impossible to achieve accurate detection of cysteine.

Method used

A trityl-based luminescent free radical material was developed, which was prepared through Friedel-Crafts alkylation, nitration and dehydrogenation reactions. It has good electron transport capability and reactivity, and can achieve three-channel response of fluorescence, colorimetric and EPR signals. For the detection of biological thiols, especially cysteine.

Benefits of technology

It achieves multiple signal detection of cysteine ​​and can perform accurate detection in RGB patterning, simulated clinical assays and nematode fluorescence imaging. It has high selectivity and sensitivity and simplifies the detection process.

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Abstract

The invention provides a triphenylmethyl light-emitting free radical material as well as a preparation method and application thereof, and belongs to the technical field of organic light-emitting materials. The triphenylmethyl free radical luminescent material provided by the invention is commonly characterized by being connected with triphenylmethyl free radicals of nitro groups with a strong electron withdrawing effect; and the triphenylmethyl free radical in the center not only serves as a luminous building group of the molecules, but also serves as an activating group, so that the triphenylmethyl free radical is more easily subjected to nucleophilic substitution by biological mercaptan. The two units (triphenylmethyl free radicals and nitryl groups) have a synergistic effect, so that the material achieves the purpose of detecting multiple responses to biological mercaptan; and detection of three channels (fluorescence, ultraviolet and EPR signals) of cysteine is realized. According to the strategy, the purpose of identifying the biological mercaptan in the aspects of RGB patterning, clinical test simulation, fluorescence imaging and the like is achieved.
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