Sample component extraction apparatus and method

Abstract

This invention relates to a sample component extraction device and method for extracting components from a sample solution within a reagent kit. The reagent kit has an open end and a closed end, and includes an adsorption unit comprising a magnetic component and an auxiliary magnet. The magnetic component extends from the open end of the reagent kit to different positions within the kit to adsorb magnetic particles in the sample solution. The auxiliary magnet is located below the closed end of the kit, and there is a variable magnetic induction intensity between the auxiliary magnet and the magnetic component. By placing the auxiliary magnet at the bottom of the kit, a variable magnetic induction intensity can be generated between the auxiliary magnet and the magnetic component extending into the kit at their relatively close ends, thereby magnetically attracting magnetic particles in the sample solution at different positions within the kit. This maximizes the extraction of magnetic particles from the sample solution and saves extraction time.

Description

Technical Field

[0001] This invention relates to the field of biomedical device technology, and in particular to a sample component extraction device and method. Background Technology

[0002] With the development of biotechnology, methods and instruments for the extraction of bioactive substances from samples and for fully automated extraction have advanced rapidly. Among them, nucleic acid extraction using magnetic beads is a new extraction technology that has been developed in recent years. The core of the magnetic beads is micron-sized iron oxide, which can be adsorbed by a magnet. The outer surface of the magnetic beads is coated with a silica film layer. In a high-salt environment, the silica film can specifically adsorb nucleic acids; in a low-salt environment, the magnetic beads coated with the silica film detach from the nucleic acids, enabling the extraction and transfer of nucleic acids.

[0003] Nucleic acid extraction kits using magnetic beads typically employ a deep-pore structure. The presence of varying pore sizes and shapes within the same deep-pore structure of the kit facilitates uniform mixing of the magnetic beads and sample solution without spillage. However, because the magnetic rods used to adsorb the beads are elongated cylindrical structures, the magnetic field is concentrated primarily in the area directly opposite the rod's end face, failing to effectively attract all beads and thus affecting the recovery rate. Increasing the number of magnetic adsorption cycles to improve recovery increases extraction time and is inefficient. Summary of the Invention

[0004] Therefore, it is necessary to provide a sample component extraction device and method to address the problem of low magnetic bead recovery rate when extracting nucleic acids using the magnetic bead method.

[0005] A sample component extraction device is disclosed for extracting components from a sample solution within a reagent kit. The reagent kit has an open end and a closed end, and includes: an adsorption unit comprising a magnetic component and an auxiliary magnet; the magnetic component is extendable from the open end of the reagent kit to different positions within the kit to adsorb magnetic particles in the sample solution; the auxiliary magnet is located below the closed end of the kit, and the auxiliary magnet has a variable magnetic induction intensity with respect to the magnetic component.

[0006] In one embodiment, the centerline of the auxiliary magnet, the centerline of the reagent kit, and the centerline of the magnetic component are located on the same straight line.

[0007] In one embodiment, the auxiliary magnet is a material that generates a magnetic field when energized or a hard magnetic material; the magnetic component is a material that generates a magnetic field when energized or a hard magnetic material.

[0008] In one embodiment, the device further includes a lifting mechanism and a control terminal. The lifting mechanism is connected to the magnetic component and the control terminal. Under the control of the control terminal, the lifting mechanism drives the magnetic component to move relative to the reagent kit.

[0009] In one embodiment, a magnet rotating assembly is further included. The control terminal is connected to the magnet rotating assembly, which is connected to the auxiliary magnet. Under the control of the control terminal, the magnet rotating assembly drives the auxiliary magnet to rotate so that it has different magnetic poles from the magnet rotating assembly at a relative position.

[0010] In one embodiment, a magnet displacement component is further included. The control terminal is connected to the magnet displacement component, and the magnet displacement component is connected to the auxiliary magnet. Under the control of the control terminal, the magnet displacement component drives the auxiliary magnet to move closer to or away from the reagent kit along the centerline direction of the reagent kit.

[0011] In one embodiment, an electronic control unit is included, the control terminal is connected to the electronic control unit, and the electronic control unit is connected to the auxiliary magnet and / or the magnetic component.

[0012] A sample component extraction method includes the following steps: S1, initializing the relative positions of the reagent kit, magnetic component, and auxiliary magnet; S2, aligning the magnetic poles at opposite ends of the magnetic component and auxiliary magnet; S3, sequentially moving the adsorption end of the magnetic component to the wide and narrow liquid surface areas of the reagent kit to adsorb magnetic particles; S4, moving the adsorption end of the magnetic component to the bottom of the reagent kit; S5, removing the force exerted by the auxiliary magnet on the magnetic component, allowing the magnetic component to adsorb the magnetic particles at the bottom of the reagent kit.

[0013] In one embodiment, step S3 further includes the following: when the adsorption end of the magnetic component is located in a narrow liquid surface region, the magnetic poles of the auxiliary magnet and the opposite ends of the magnetic component are reversed.

[0014] In one embodiment, at least one of the following is included:

[0015] The auxiliary magnet is an electromagnet. Methods to remove the force exerted by the auxiliary magnet on the magnetic components include: de-energizing the auxiliary magnet, or moving the auxiliary magnet away from the kit along the centerline of the kit.

[0016] The auxiliary magnet is a permanent magnet. Methods to remove the force exerted by the auxiliary magnet on the magnetic components include: moving the auxiliary magnet along the centerline of the kit to move it away from the kit.

[0017] The above-mentioned sample component extraction device and method, by setting an auxiliary magnet at the bottom of the reagent kit, can generate varying magnetic induction intensity between the auxiliary magnet and the magnetic component extending into the reagent kit at relatively close ends, thereby magnetically attracting magnetic particles in the sample solution at different locations in the reagent kit, and can maximize the extraction of magnetic particles in the sample solution. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of the reagent kit in one embodiment of the present invention.

[0019] Figure 2 This is a cross-sectional view of the reagent kit and magnetic components in conjunction in one embodiment of the present invention.

[0020] Figure 3 This is a cross-sectional view of the magnetic component and the reagent kit in different mating positions according to an embodiment of the present invention.

[0021] Figure 4 This is a schematic diagram showing the distribution of magnetic field lines between the magnetic component and the auxiliary magnet in one embodiment of the present invention. Detailed Implementation

[0022] This invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this invention. Therefore, this invention is not limited to the specific embodiments disclosed below.

[0023] In the description of this invention, the terms "vertical," "horizontal," "upper," "lower," "left," "right," "center," "longitudinal," "lateral," and "width," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing the invention and simplifying the description. "Above" or "below" the second feature can mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0024] In this invention, unless otherwise expressly specified and limited, the terms "installed," "connected," "attached," "fixed," etc., shall be interpreted broadly. When an element is referred to as being "fixed to" or "set on" another element, it may be directly on the other element or there may be an intervening element. When an element is considered to be "connected" to another element, it may be directly connected to the other element or there may be an intervening element.

[0025] Example 1

[0026] One embodiment of the present invention provides a sample component extraction device for extracting components from a sample solution within a reagent kit 100. The sample solution contains magnetic particles that adsorb the desired sample components. The sample extraction device generates a magnetic field to adsorb the magnetic particles, thereby separating and extracting the sample components from the sample solution. In this embodiment, the magnetic particles are magnetic beads. The sample component to be extracted from the sample solution is nucleic acid.

[0027] See Figure 1 As shown, the reagent kit 100 has an open end 110, a closed end 120, and a hollow chamber 130, which is connected to the opening of the open end 110. The chamber 130 is used to hold sample solutions for component extraction by a sample component extraction device.

[0028] See Figure 2 As shown, the reagent kit 100 has a set length and different cross-sectional dimensions along its length. For ease of understanding and description, the reagent kit 100 is divided into a first tube segment 101, a second tube segment 102, and a bottom tube segment 103 sequentially from the open end 110 to the closed end 120. The cross-sectional dimension of the first tube segment 101 is larger than that of the second tube segment 102, and the cross-sectional dimension of the second tube segment 102 is larger than that of the bottom tube segment 103. The first tube segment 101, where the sample solution is located, is referred to as the wide liquid surface region, the second tube segment 102, where the sample solution is located, is referred to as the narrow liquid surface region, and the bottom tube segment 103, where the sample solution is located, is referred to as the bottom end of the reagent kit 100.

[0029] The reagent kit 100 can be a single unit or multiple reagent kits 100 can be combined into a reagent kit component.

[0030] The sample component extraction device is equipped with a fixed support for supporting and fixing individual reagent kits 100 or reagent kit components composed of multiple reagent kits 100.

[0031] See Figure 2 As shown, the sample component extraction device includes an adsorption unit 300, which includes a magnetic component 500 capable of generating a magnetic field for magnetic adsorption of magnetic particles within the magnetic adsorption kit 100. The magnetic component 500 includes a magnetic rod 510 and a magnetic rod sheath 520. The magnetic rod sheath 520 is fitted onto the magnetic rod 510 to prevent the magnetic rod 510 from directly contacting the magnetic particles.

[0032] The magnetic component 500 has two opposing ends, one of which is used for immersion in the sample solution and is called the adsorption end.

[0033] The magnetic rod sheath 520 is made of a non-magnetic material, such as insulating materials like polypropylene (PP). The magnetic rod sheath 520 is a hollow cylinder with a closed bottom, or a structure adapted to the magnetic rod 510. Its thickness is designed to not weaken the magnetic field strength, or to have a minimal impact on the magnetic field strength, and to still generate a magnetic field capable of attracting magnetic particles after the magnetic rod 510 is inserted.

[0034] The magnetic rod 510 can be inserted into or detached from the magnetic rod sheath 520. After the magnetic rod 510 is inserted into the magnetic rod sheath 520, the magnetic particles attracted by the magnet adhere to the magnetic rod sheath 520. (28 segments)

[0035] In this embodiment, the magnetic component 500 corresponds one-to-one with the reagent kit 100, and the magnetic rod 510 is made of hard magnetic material. In other embodiments, the magnetic rod 510 can be an electromagnet, and its magnetic field strength or magnetic pole direction can be controlled by energizing it.

[0036] During nucleic acid extraction, the magnetic component 500 needs to move up and down relative to the reagent kit 100. The sample component extraction device is equipped with a lifting mechanism (not shown). The lifting mechanism is located above the magnetic component 500 and is connected to the magnetic component 500 in a transmission manner.

[0037] The lifting mechanism has a power source, which can be a device capable of providing power, such as a motor, cylinder, or hydraulic cylinder. The power source transmits the output power to the magnetic component 500 through a corresponding transmission mechanism. The transmission mechanism can be any mechanism that enables the magnetic component 500 to move upward and downward, such as a lead screw and nut transmission pair.

[0038] The sample component extraction device is equipped with a control terminal, and the lifting mechanism is connected to the control terminal to receive control commands issued by the control terminal and execute corresponding actions.

[0039] Because the reagent kit 100 has different cross-sectional areas, the sample solution will exhibit different liquid level widths when it is filled into the chamber 120 of the reagent kit 100. The magnetic particles in the sample solution are distributed in different areas; that is, the magnetic particles are at different distances from the center line of the reagent kit 100. On the other hand, the magnetic rod 510 used to generate the magnetic field mainly concentrates its magnetic field at its end. Therefore, when the magnetic component 500 extends into the reagent kit 100 to adsorb magnetic particles, the adsorption effect of the magnetic component 500 on the magnetic beads is poor in areas with larger liquid level widths, resulting in some nucleic acids attached to the magnetic beads not being extracted, affecting the experimental results. Repeated extraction is time-consuming. (See also...) Figure 2As shown, in one embodiment, the adsorption unit 300 is provided with an auxiliary magnet 700. The auxiliary magnet 700 is used to cooperate with the magnetic component 500 so that the magnetic induction intensity between the two opposite ends of the auxiliary magnet 700 and the magnetic component 500 is variable, so that the magnetic component 500 can adsorb magnetic particles at different liquid surface widths, thereby improving the magnetic particle recovery rate.

[0040] The two opposite ends of the magnetic component 500 and the auxiliary magnet 700 refer to the end of the magnetic component 500 facing the closed end 120 of the reagent kit 100, that is, the adsorption end and the end of the auxiliary magnet 700 facing the closed end 120 of the reagent kit 100.

[0041] See Figure 2 As shown, the auxiliary magnet 700 is located below the closed end 120 of the kit 100. The centerline of the auxiliary magnet 700 is on the same straight line as the centerline of the kit 100 and the centerline of the magnetic component 500.

[0042] In this embodiment, the auxiliary magnet 700 is an electromagnet that generates a magnetic field by energizing and demagnetizes by de-energizing. Accordingly, the sample component extraction device is equipped with an electronic control unit, which is connected to a control terminal to receive control commands from the control terminal to perform operations such as energizing or de-energizing the auxiliary magnet 700.

[0043] The method for extracting sample components includes the following steps:

[0044] S1. The relative positions of the initialization kit 100, magnetic component 500, and auxiliary magnet 700.

[0045] Install the magnetic component 500 onto the lifting mechanism, and fix the reagent kit 100 in the set position. Position the magnetic component 500 directly above the reagent kit 100, and position the auxiliary magnet 700 directly below the reagent kit 100. After initialization, the center lines of the reagent kit 100, the magnetic component 500, and the auxiliary magnet 700 are aligned on the same straight line.

[0046] S2. Energize the auxiliary magnet 700, and the magnetic poles at the opposite ends of the auxiliary magnet 700 and the magnetic component 500 are the same after being energized.

[0047] See Figure 2 As shown, by way of example, the end of the magnetic component 500 facing the closed end 120 of the reagent kit 100 is the N pole, and similarly, the end of the auxiliary magnet 700 facing the closed end 120 of the reagent kit 100 is also the N pole.

[0048] S3. Insert the magnetic component 500 into the reagent kit 100, and let the adsorption end of the magnetic component 500 pass through the wide liquid surface area and the narrow liquid surface area of ​​the sample solution in sequence.

[0049] Driven by the lifting mechanism, the magnetic component 500 extends into the reagent kit 100 and gradually approaches the closed end 120 from the open end 110 of the reagent kit 100 to adsorb magnetic particles in the sample solution.

[0050] During the magnetic attraction process in which the magnetic component 500 gradually approaches the closed end 120 from the open end 110, the magnetic component 500 can move continuously, that is, gradually pass through the first tube segment 101 and the second tube segment 102 at a set speed; it can also pause briefly at a set position in the first tube segment 101 and / or the second tube segment 102. Here, the movement mode of the magnetic component 500 is not specifically limited.

[0051] Based on the phenomenon that like poles repel each other, see [reference needed]. Figure 4 As shown in (a), when the magnetic poles at both ends of the auxiliary magnet 700 and the magnetic component 500 are the same, the magnetic field lines of the magnetic component 500 deflect away from the center line, and the magnetic field lines of the auxiliary magnet 700 also deflect away from the center line. At this time, the lateral magnetic field strength of both the magnetic component 500 and the auxiliary magnet 700 increases. The magnetic component 500 has a greater magnetic attraction to magnetic particles that are not directly below it. This can improve the recovery rate of magnetic particles far from the end of the magnetic component 500.

[0052] Meanwhile, since the auxiliary magnet 700 is located at the bottom of the reagent kit 100, it generates a magnetic field when energized, and some magnetic particles will be attracted by the auxiliary magnet 700 and gather at the bottom tube 103 of the reagent kit 100.

[0053] S4. Move the adsorption end of the magnetic component 500 to the bottom of the reagent kit 100.

[0054] See also Figure 3 As shown in (b), when the adsorption end of the magnetic component 500 is located at the bottom of the kit 100, that is, at the bottom tube segment 103, since the bottom tube segment 103 is narrow, the sample solution in the bottom tube segment 103 is basically located directly below the magnetic component 500, and the magnetic particles are located directly below the adsorption end of the magnetic component 500.

[0055] S5. De-energize the auxiliary magnet 700 and wait for the magnetic component 500 to complete the magnetic attraction of the magnetic particles at the bottom of the reagent kit 100.

[0056] After the auxiliary magnet 700 is de-energized, the magnetic field lines distribution at the attraction end of the magnetic component 500 returns to normal. (See reference...) Figure 4 As shown in (b), the magnetic field strength is greatest directly below the magnetic component 500. At this time, the magnetic component 500 can effectively adsorb the magnetic particles that are gathered at the bottom of the reagent kit 100.

[0057] To better and more effectively adsorb magnetic particles in different sections of the reagent kit 100, step S3 specifically includes the following: when the adsorption end of the magnetic component 500 is located in a narrow liquid surface region, the direction of current flow to the auxiliary magnet 700 is reversed, thereby making the magnetic poles of the auxiliary magnet 700 and the magnetic component 500 opposite. For example, see [reference needed]. Figure 3 As shown in (a), the end of the auxiliary magnet 700 facing the closed end 120 of the reagent kit 100 is the S pole. At the same time, since the auxiliary magnet 700 generates a magnetic field when energized, some magnetic particles will be attracted by the auxiliary magnet 700 and accumulate on the bottom tube section 103 of the reagent kit 100.

[0058] Based on the phenomenon that opposite poles attract each other, please refer to... Figure 4 As shown in (c), the region between the magnetic component 500 and the auxiliary magnet 700 has relatively concentrated magnetic field lines and a larger magnetic field strength. This is more conducive to the adsorption of magnetic particles in a narrow liquid surface area.

[0059] By changing the direction of the magnetic field of the auxiliary magnet 700, that is, by making the magnetic component 500 and the auxiliary magnet 700 have different magnetic poles at opposite ends, an interaction between the magnetic poles is generated, thereby forming different magnetic field strengths at different positions of the sample liquid, improving the recovery rate of magnetic particles in the kit 100, and saving extraction time.

[0060] It should be noted that when the auxiliary magnet 700 is an electromagnet, the magnetic field strength of the auxiliary magnet 700 can be changed by changing the magnitude of the current.

[0061] Example 2

[0062] Unlike Example 1, in this example, the auxiliary magnet 700 is a permanent magnet.

[0063] The sample component extraction device includes a magnet rotation assembly (not shown) and a magnet displacement assembly (not shown).

[0064] The magnet rotation assembly is connected to the auxiliary magnet 700 for driving the auxiliary magnet 700 to rotate relative to the kit 100, so that the end of the auxiliary magnet 700 near the bottom of the kit 100 has the same or different magnetic poles as the end of the magnetic assembly 500 located in the kit 100 near the bottom of the kit 100.

[0065] The magnet rotation assembly has a power element, which can be a device capable of providing power, such as a motor, cylinder, or hydraulic cylinder. The power element transmits the output power to the auxiliary magnet 700 through a corresponding transmission assembly, causing it to rotate. The transmission assembly can be a shaft drive or gear drive, or any structure that enables the auxiliary magnet 700 to rotate.

[0066] The magnet displacement assembly is connected to the auxiliary magnet 700 via a transmission mechanism, and is used to move the auxiliary magnet 700 up and down relative to the reagent kit 100 to change the magnetic field strength between the auxiliary magnet 700 and the magnetic component 500. The magnet displacement assembly includes a drive assembly, which transmits output power to the auxiliary magnet 700 via a transmission unit to move it up and down. The drive assembly can be a motor, etc., and the transmission unit can be a belt drive structure or a slider guide structure, etc., to move the auxiliary magnet 700 up and down.

[0067] Both the magnet rotation component and the magnet displacement component are connected to the control terminal to receive control commands from the control terminal and execute corresponding actions.

[0068] When the auxiliary magnet 700 is a permanent magnet, the magnetic rod 510 is a permanent magnet, and the magnetic components 500 correspond one-to-one with the reagent kit 100, the method for extracting sample components includes the following steps:

[0069] S1. Initialize the relative positions of the kit 100, magnetic component 500, and auxiliary magnet 700; the magnetic poles at opposite ends of the magnetic component 500 and auxiliary magnet 700 are the same.

[0070] The magnetic component 500 is located directly above the reagent kit 100, and the auxiliary magnet 700 is located directly below the reagent kit 100. The center line of the reagent kit 100, the center line of the magnetic component 500, and the center line of the auxiliary magnet 700 are all on the same straight line.

[0071] As an example, see Figure 2 As shown, after initialization, the end of the magnetic component 500 facing the closed end 120 of the reagent kit 100 is the N pole, and similarly, the end of the auxiliary magnet 700 facing the closed end 120 of the reagent kit 100 is also the N pole.

[0072] S2. Insert the magnetic component 500 into the reagent kit 100, and let the adsorption end of the magnetic component 500 pass through the wide liquid surface area and the narrow liquid surface area of ​​the sample solution in sequence to complete the adsorption of magnetic particles in the wide liquid surface area and the narrow liquid surface area.

[0073] Similar to Example 1, some magnetic particles will be attracted by the auxiliary magnet 700 and gather in the bottom tube 103 of the kit 100.

[0074] S3. Move the adsorption end of the magnetic component 500 to the bottom of the reagent kit 100.

[0075] S4. Move the auxiliary magnet 700 downwards to a position away from the reagent kit 100 using the magnet displacement component, so that there is no interaction between the magnetic component 500 and the auxiliary magnet 700; wait for the magnetic component 500 to complete the magnetic attraction of the magnetic particles at the bottom of the reagent kit 100.

[0076] It should be noted that during step S2, as the magnetic component 500 gradually approaches the bottom of the reagent kit 100, the auxiliary magnet 700 can also be displaced in the vertical direction as needed.

[0077] Similar to Example 1, in order to better and more effectively adsorb magnetic particles in different sections of the reagent kit 100, step S2 specifically includes the following: when the adsorption end of the magnetic component 500 is located in a narrow liquid surface area, the auxiliary magnet 700 is rotated 180° by the magnet rotation component, thereby making the magnetic poles of the auxiliary magnet 700 opposite to those of the magnetic component 500 at opposite ends. See also... Figure 3 As shown in (a), the end of the rotated auxiliary magnet 700 facing the closed end 120 of the reagent kit 100 is the S pole. At the same time, some magnetic particles will be attracted by the auxiliary magnet 700 and accumulate on the bottom tube section 103 of the reagent kit 100.

[0078] It should be noted that in Embodiments 1 and 2 above, the magnetic rod 510 is selected as a hard magnetic material, but is not limited to hard magnetic materials. The magnetic rod 510 can be made of materials such as electromagnets that can change the magnetic field strength and magnetic pole direction. Similarly, the magnetic poles at the opposite ends of the magnetic component 500 and the auxiliary magnet 700 can be changed by changing the magnetic poles of the magnetic rod 510, or the magnetic field strength between the magnetic component 500 and the auxiliary magnet 700 can be changed by changing the magnitude of the magnetic field strength of the magnetic rod 510. Any scheme that can achieve the change of magnetic poles or magnetic field strength at the opposite ends of the magnetic component 500 and the auxiliary magnet 700 when the magnetic component 500 is in different liquid surface width regions within the reagent kit 100 is acceptable.

[0079] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0080] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A sample component extraction device for extracting components from a sample solution within a reagent kit, the reagent kit having opposing open and closed ends, characterized in that, include: An adsorption unit is provided, comprising a magnetic component and an auxiliary magnet; the magnetic component can extend from the open end of the reagent kit to different positions on the reagent kit to adsorb magnetic particles in the sample solution; the auxiliary magnet is located below the closed end of the reagent kit, and the auxiliary magnet and the magnetic component have a variable magnetic induction intensity. The reagent kit is divided into a first tube segment, a second tube segment, and a bottom tube segment in sequence from the open end to the closed end. The cross-sectional dimension of the first tube segment is larger than that of the second tube segment, and the cross-sectional dimension of the second tube segment is larger than that of the bottom tube segment. The first tube segment where the sample solution is located is called the wide liquid surface area, the second tube segment where the sample solution is located is called the narrow liquid surface area, and the bottom tube segment where the sample solution is located is called the bottom end of the reagent kit.

2. The sample component extraction device according to claim 1, characterized in that, The centerline of the auxiliary magnet, the centerline of the reagent kit, and the centerline of the magnetic component are all on the same straight line.

3. The sample component extraction device according to claim 1, characterized in that, The auxiliary magnet is a material that generates a magnetic field when energized or a hard magnetic material; the magnetic component is a material that generates a magnetic field when energized or a hard magnetic material.

4. The sample component extraction device according to claim 1, characterized in that, It also includes a lifting mechanism and a control terminal. The lifting mechanism is connected to the magnetic component and the control terminal. Under the control of the control terminal, the lifting mechanism drives the magnetic component to move relative to the reagent kit.

5. The sample component extraction device according to claim 4, characterized in that, It also includes a magnet rotating assembly, the control terminal is connected to the magnet rotating assembly, the magnet rotating assembly is connected to the auxiliary magnet, and the magnet rotating assembly drives the auxiliary magnet to rotate under the control of the control terminal so that it has different magnetic poles relative to the magnet assembly.

6. The sample component extraction device according to claim 4, characterized in that, It also includes a magnet displacement component, the control terminal is connected to the magnet displacement component, the magnet displacement component is connected to the auxiliary magnet, and the magnet displacement component, under the control of the control terminal, drives the auxiliary magnet to move closer to or away from the reagent kit along the centerline direction of the reagent kit.

7. The sample component extraction device according to claim 4, characterized in that, It includes an electronic control unit, the control terminal is connected to the electronic control unit, and the electronic control unit is connected to the auxiliary magnet and / or the magnetic component.

8. A method for extracting sample components, characterized in that, Extracting sample components using the sample component extraction apparatus according to any one of claims 1 to 7 includes the following steps: S1. The relative positions of the initialization kit, magnetic components, and auxiliary magnet; S2. Make the magnetic poles at opposite ends of the magnetic component and the auxiliary magnet the same; S3. The adsorption end of the magnetic component moves sequentially to the wide liquid surface area and the narrow liquid surface area of ​​the reagent kit to adsorb magnetic particles. S4. The adsorption end of the magnetic component moves to the bottom of the kit; S5. Remove the force exerted by the auxiliary magnet on the magnetic component, allowing the magnetic component to adhere to the magnetic particles at the bottom of the reagent kit.

9. The sample component extraction method according to claim 8, characterized in that, Step S3 also includes the following: When the adsorption end of the magnetic component is located in a narrow liquid surface region, the magnetic poles of the auxiliary magnet and the opposite ends of the magnetic component are reversed.

10. The sample component extraction method according to claim 8, characterized in that, Includes at least one of the following: The auxiliary magnet is an electromagnet. Methods to remove the force exerted by the auxiliary magnet on the magnetic components include: de-energizing the auxiliary magnet, or moving the auxiliary magnet away from the kit along the centerline of the kit. The auxiliary magnet is a permanent magnet. Methods to remove the force exerted by the auxiliary magnet on the magnetic components include: moving the auxiliary magnet along the centerline of the kit to move it away from the kit.

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