Injectable hemostatic cryogel for promoting tissue regeneration and preparation method and application thereof

Abstract

The invention discloses injectable hemostatic cryogel for promoting tissue regeneration and a preparation method and application thereof. The preparation method comprises the following steps: ultrasonically dispersing polydopamine, chitosan and collagen in deionized water, adding glacial acetic acid, and stirring until dissolving; and dissolving oxidized dextran in deionized water, adding an oxidized dextran solution into a mixed solution under vigorous stirring, transferring to a refrigerator for a reaction, and melting at room temperature to obtain the injectable hemostatic cryogel for promoting tissue regeneration. The aperture of the cryogel is 50-200 [mu] m, and the cryogel is provided with interconnected macroporous structures and stable mechanical strength, can be conveyed to a narrow and deep wound through an injector after being compressed and fixed, quickly recovers to block the wound and form a physical barrier after sucking blood, and activates a blood coagulation process through concentrated blood to achieve a hemostasis effect. The cryogel prepared by the invention has rapid shape recovery capability, injectability, good hemostatic capability and the like.

Description

technical field

[0001] The invention belongs to the technical field of biomedical materials, and in particular relates to an injectable hemostatic gel for promoting tissue regeneration, a preparation method and application thereof. Background technique

[0002] Bleeding complications from trauma, surgery, congenital disorders, or drug-induced blood disorders result in significant morbidity and mortality. Hemorrhage has been reported to be responsible for more than 30% of traumatic mortality. Therefore, timely hemostasis is of great significance in emergency situations such as battlefields, operations, and car accidents. In these emergencies, rapid hemostasis cannot be achieved by the body's hemostatic mechanisms alone. Appropriate hemostatic agents are therefore needed to promote, enhance, compensate or mimic the natural mechanisms of hemostasis. Non-compressive bleeding accounts for 50% of deaths from bleeding. In the battlefield, wounds caused by bullets, bombs, and ex...

Examples

Embodiment 1

[0038] 1 mL of ammonia solution, 20 mL of absolute ethanol and 45 mL of deionized water were stirred at room temperature for 20 minutes. Then, 5 mL of dopamine hydrochloride solution with a concentration of 50 mg / mL was quickly added to the above mixed solution. After 1 minute, the solution color changed to pale yellow. After vigorous stirring at room temperature for 20 hours, the resulting solution turned dark brown. Finally, polydopamine nanoparticles were obtained by centrifugation and washed three times with deionized water. The product is detected by scanning electron microscopy as nanospheres with a particle size of about 200nm to 300nm, such as figure 1 shown.

Embodiment 2

[0040] 1.5 mL of ammonia solution, 20 mL of absolute ethanol and 40 mL of deionized water were stirred at room temperature for 30 minutes. Then, 4 mL of dopamine hydrochloride solution with a concentration of 40 mg / mL was quickly added to the above mixed solution. After 1 minute, the solution color changed to pale yellow. After vigorous stirring at room temperature for 12 hours, the resulting solution turned dark brown. Finally, polydopamine nanoparticles were obtained by centrifugation and washed three times with deionized water. Its physical and chemical properties are similar to Example 1.

Embodiment 3

[0042] Stir 0.5 mL of ammonia solution, 20 mL of absolute ethanol, and 30 mL of deionized water at room temperature for 10 minutes. Then, 6 mL of dopamine hydrochloride solution with a concentration of 20 mg / mL was quickly added to the above mixed solution. After 1 minute, the solution color changed to light yellow. After vigorous stirring at room temperature for 24 hours, the resulting solution turned dark brown. Finally, polydopamine nanoparticles were obtained by centrifugation and washed three times with deionized water. Its physical and chemical properties are similar to Example 1.

[0043] Preparation of oxidized dextran

technical field

[0001] The invention belongs to the technical field of biomedical materials, and in particular relates to an injectable hemostatic gel for promoting tissue regeneration, a preparation method and application thereof. Background technique

[0002] Bleeding complications from trauma, surgery, congenital disorders, or drug-induced blood disorders result in significant morbidity and mortality. Hemorrhage has been reported to be responsible for more than 30% of traumatic mortality. Therefore, timely hemostasis is of great significance in emergency situations such as battlefields, operations, and car accidents. In these emergencies, rapid hemostasis cannot be achieved by the body's hemostatic mechanisms alone. Appropriate hemostatic agents are therefore needed to promote, enhance, compensate or mimic the natural mechanisms of hemostasis. Non-compressive bleeding accounts for 50% of deaths from bleeding. In the battlefield, wounds caused by bullets, bombs, and ex...

Examples

Embodiment 1

[0038] 1 mL of ammonia solution, 20 mL of absolute ethanol and 45 mL of deionized water were stirred at room temperature for 20 minutes. Then, 5 mL of dopamine hydrochloride solution with a concentration of 50 mg / mL was quickly added to the above mixed solution. After 1 minute, the solution color changed to pale yellow. After vigorous stirring at room temperature for 20 hours, the resulting solution turned dark brown. Finally, polydopamine nanoparticles were obtained by centrifugation and washed three times with deionized water. The product is detected by scanning electron microscopy as nanospheres with a particle size of about 200nm to 300nm, such as figure 1 shown.

Embodiment 2

[0040] 1.5 mL of ammonia solution, 20 mL of absolute ethanol and 40 mL of deionized water were stirred at room temperature for 30 minutes. Then, 4 mL of dopamine hydrochloride solution with a concentration of 40 mg / mL was quickly added to the above mixed solution. After 1 minute, the solution color changed to pale yellow. After vigorous stirring at room temperature for 12 hours, the resulting solution turned dark brown. Finally, polydopamine nanoparticles were obtained by centrifugation and washed three times with deionized water. Its physical and chemical properties are similar to Example 1.

Embodiment 3

[0042] Stir 0.5 mL of ammonia solution, 20 mL of absolute ethanol, and 30 mL of deionized water at room temperature for 10 minutes. Then, 6 mL of dopamine hydrochloride solution with a concentration of 20 mg / mL was quickly added to the above mixed solution. After 1 minute, the solution color changed to light yellow. After vigorous stirring at room temperature for 24 hours, the resulting solution turned dark brown. Finally, polydopamine nanoparticles were obtained by centrifugation and washed three times with deionized water. Its physical and chemical properties are similar to Example 1.

[0043] Preparation of oxidized dextran

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