Sample detection apparatus and method

Abstract

A sample detection device and method thereof, specifically, after each time the detection of the mixed sample liquid in the reaction pool is controlled, a regular cleaning mode is executed to clean the reaction pool so that the reaction pool can detect the specific protein content of the next sample; when the preset temporary cleaning condition is reached, a temporary cleaning mode is executed to clean the reaction pool; when the preset periodic cleaning opportunity is reached, a deep cleaning mode is executed to clean the reaction pool. The application introduces three cleaning modes: regular cleaning mode, temporary cleaning mode and deep cleaning mode, which can make the reaction pool maintain the cleaning state for a long time.

Description

TECHNICAL FIELD

[0001] The present application relates to a sample detection device and a method thereof. BACKGROUND

[0002] With the popularization of clinical application, more and more parameters need to be detected in the field of blood test. From the initial three-classification and five-classification of blood routine, to the later simultaneous detection of blood routine and special protein detection. At present, the detection of special protein is gradually popularized. In addition to blood routine + CRP (C-Reactive Protein, C-reactive protein), there are blood routine + SAA, blood routine + CRP + SAA and even more parameters (such as PCT, IL6, etc.). In order to realize the ease of detection, it is generally necessary to complete the detection of the above parameters with one blood sample, that is, to realize the blood routine and the special protein integrated machine.

[0003] The detection of special protein generally adopts transmission and / or scattering turbidimetry. To detect the concentration of special protein (antigen) in blood, special latex particles (antibody) need to be added to the sample. The latex particle is a nanoscale spherical particle, which can combine with the surrounding special protein under certain conditions to form a larger volume of micelle. When the latex particles continuously combine with the special protein, the micelle assembly formed increases, and the scattering signal assembly formed after irradiation by a specific wavelength of light increases, and the transmission signal gradually weakens. By monitoring the rate of change of transmission and / or scattering signals, and through certain calculation, the content of special protein in the sample can be obtained.

[0004] According to the above introduction, the essence of the reaction of special protein detection is that the special protein antigen and antibody latex microspheres continuously combine to form larger micelles. These protein micelles are easily adhered to the pool wall of the reaction pool during the formation process. When the test is completed, the pool wall needs to be cleaned to restore it to a measurement-ready state to avoid affecting the detection results of the next sample.

[0005] Therefore, the cleaning of the reaction pool for special protein detection is a necessary factor to ensure the accuracy of the detection results of special protein. SUMMARY

[0006] The present application provides a sample detection device and a method thereof, which will be described in detail below.

[0007] According to a first aspect, a sample detection device is provided in an embodiment, comprising:

[0008] A sample mechanism for providing a sample to be tested;

[0009] A reagent mechanism for providing a reagent for special protein detection and providing a reagent for blood routine detection, the reagent for blood routine detection comprising one or more hemolytic agents;

[0010] a blood routine component for processing a sample to be tested provided by a sample mechanism with reagents for blood routine test provided by the reagent mechanism, so as to perform cell classification and / or counting on the sample to be tested;

[0011] a reaction cell for receiving the sample to be tested provided by the sample mechanism and reagents for specific protein test provided by the reagent mechanism, so that the two react to form a mixed sample liquid;

[0012] a specific protein test component including a test area made of a light-transmitting material, a light source and a receiver arranged correspondingly to the test area, the light source being used to irradiate the mixed sample liquid flowing through the test area, so that the receiver senses a light signal related to the content of specific protein in the mixed sample liquid, the light signal being used to detect the content of specific protein in the mixed sample liquid;

[0013] a cleaning assembly for cleaning at least the reaction cell; the cleaning assembly has three cleaning modes: a normal cleaning mode, a temporary cleaning mode and a deep cleaning mode, and at least one of the cleaning modes uses the one or more hemolytic agents as cleaning liquid to clean the reaction cell;

[0014] a controller for controlling the cleaning assembly to clean the reaction cell, specifically including:

[0015] each time after the mixed sample liquid in the reaction cell is tested, the controller controls the cleaning assembly to perform the normal cleaning mode to clean the reaction cell, so that the reaction cell can perform the detection of the content of specific protein of the next sample;

[0016] when a preset temporary cleaning condition is reached, the controller controls the cleaning assembly to perform the temporary cleaning mode to clean the reaction cell;

[0017] when a preset periodic cleaning opportunity is reached, the controller controls the cleaning assembly to perform the deep cleaning mode to clean the reaction cell.

[0018] In an embodiment, the reaction cell is provided with a waste liquid discharge port and a first cleaning port;

[0019] The sample testing device further includes a waste liquid channel and a waste liquid driving component; one end of the waste liquid channel is connected with the waste liquid discharge port of the reaction cell, and the other end is connected with the waste liquid driving component; the waste liquid driving component is used to discharge the liquid in the reaction cell through the waste liquid channel;

[0020] The cleaning assembly further comprises a cleaning liquid supply component and a cleaning liquid supply component; the cleaning liquid supply component comprises a cleaning liquid supply channel connected with the first cleaning port of the reaction pool, and the cleaning liquid supply component is configured to supply cleaning liquid to the reaction pool through the cleaning liquid supply channel; and the cleaning liquid supply component is configured to supply the cleaning liquid to the reaction pool.

[0021] The first cleaning port is arranged eccentrically, so that the cleaning liquid supplied by the cleaning liquid supply component enters the reaction pool from the first cleaning port in an eccentric direction.

[0022] In an embodiment, the first cleaning port is arranged in a tangential direction of the wall of the reaction pool.

[0023] In an embodiment, the cleaning liquid supply component further comprises a recovery channel, one end of which is connected with the waste liquid discharge port, and the other end of which is connected with the first cleaning port, and the recovery channel is configured to recover the cleaning liquid discharged from the waste liquid discharge port and re-use the cleaning liquid as cleaning liquid for flushing the reaction pool.

[0024] In an embodiment, the reaction pool is provided with a second cleaning port; the cleaning liquid supply component comprises a cleaning liquid supply channel connected with the second cleaning port of the reaction pool, and the cleaning liquid supply component is configured to supply the cleaning liquid to the reaction pool through the cleaning liquid supply channel.

[0025] The second cleaning port is arranged eccentrically, so that the cleaning liquid supplied by the cleaning liquid supply component enters the reaction pool from the second cleaning port in an eccentric direction.

[0026] In an embodiment, the second cleaning port is arranged in a tangential direction of the wall of the reaction pool.

[0027] In an embodiment, the cleaning liquid supply component re-uses the reagent mechanism to supply one or more hemolytic agents as the cleaning liquid to the reaction pool during cleaning.

[0028] In an embodiment, the time consumption of one routine cleaning mode is less than that of one temporary cleaning mode, and the time consumption of one temporary cleaning mode is less than or equal to that of one deep cleaning mode.

[0029] According to a second aspect, an embodiment provides a method of a sample detection device, comprising:

[0030] controlling to draw a sample to be tested, and respectively discharge a part of the sample to be tested into a blood routine reaction pool and a reaction pool for specific protein detection;

[0031] controlling to add one or more hemolytic agents into the blood routine reaction pool;

[0032] controlling detection of at least one item in a blood routine test on the sample to be tested treated by the hemolytic agent;

[0033] controlling adding of a latex reagent for reaction into the reaction pool for specific protein detection to prepare a mixed sample liquid;

[0034] controlling irradiation on the mixed sample liquid to detect the specific protein content in the mixed sample liquid;

[0035] controlling execution of cleaning on the reaction pool, wherein the method comprises three cleaning modes of the reaction pool: a regular cleaning mode, a temporary cleaning mode and a deep cleaning mode, and wherein at least one cleaning mode uses the one or more hemolytic agents as cleaning liquid to clean the reaction pool; the controlling execution of cleaning on the reaction pool comprises:

[0036] controlling execution of the regular cleaning mode to clean the reaction pool after each time of controlling detection of the mixed sample liquid in the reaction pool, so that the reaction pool can be used for detection of specific protein content of the next sample;

[0037] controlling execution of the temporary cleaning mode to clean the reaction pool when a preset temporary cleaning condition is reached;

[0038] controlling execution of the deep cleaning mode to clean the reaction pool when a preset periodic cleaning opportunity is reached.

[0039] According to a third aspect, an embodiment provides a method of a sample detection device, comprising:

[0040] controlling suction of a sample to be tested and discharging the sample to be tested into a reaction pool for specific protein detection;

[0041] controlling adding of a latex reagent for reaction into the reaction pool for specific protein detection to prepare a mixed sample liquid;

[0042] controlling irradiation on the mixed sample liquid to detect the specific protein content in the mixed sample liquid;

[0043] controlling execution of cleaning on the reaction pool, wherein the method comprises three cleaning modes of the reaction pool: a regular cleaning mode, a temporary cleaning mode and a deep cleaning mode; the controlling execution of cleaning on the reaction pool comprises:

[0044] controlling execution of the regular cleaning mode to clean the reaction pool after each time of controlling detection of the mixed sample liquid in the reaction pool, so that the reaction pool can be used for detection of specific protein content of the next sample;

[0045] When a preset temporary cleaning condition is reached, the control executes a temporary cleaning mode to clean the reaction cell;

[0046] When a preset periodic cleaning opportunity is reached, the control executes a deep cleaning mode to clean the reaction cell.

[0047] In one embodiment, the regular cleaning mode comprises:

[0048] The control flushes the reaction cell with cleaning liquid.

[0049] In one embodiment, the control flushes the reaction cell with cleaning liquid comprises:

[0050] The control adds cleaning liquid to the reaction cell in a direction eccentric to the tangent of the wall of the reaction cell.

[0051] In one embodiment, the regular cleaning mode further comprises:

[0052] The control flushes the reaction cell with cleaning liquid while constantly discharging the cleaning liquid in the reaction cell as waste liquid, or

[0053] The control flushes the reaction cell with cleaning liquid while constantly discharging the cleaning liquid in the reaction cell and recycling it as cleaning liquid for flushing the reaction cell.

[0054] In one embodiment, the regular cleaning mode further comprises:

[0055] The control adds cleaning liquid to the reaction cell and soaks the reaction cell for a preset time before discharging it as waste liquid.

[0056] In one embodiment, the cleaning liquid comprises one or more hemolytic agents.

[0057] In one embodiment, the deep cleaning mode comprises:

[0058] The control adds one or more cleaning liquids to the reaction cell and soaks the reaction cell for a preset time before discharging it as waste liquid.

[0059] In one embodiment, the deep cleaning mode further comprises:

[0060] The control flushes the reaction cell with cleaning liquid or the cleaning liquid.

[0061] In one embodiment, the deep cleaning mode further comprises:

[0062] The control flushes the reaction cell with cleaning liquid / the cleaning liquid while constantly discharging the cleaning liquid / the cleaning liquid in the reaction cell as waste liquid, or

[0063] controlling flushing of the reaction cell with the washing liquid / the cleaning liquid while continuously discharging and recycling the washing liquid / the cleaning liquid in the reaction cell as the washing liquid / cleaning liquid for flushing the reaction cell.

[0064] In one embodiment, the preset periodic cleaning timing includes any one or more of the following:

[0065] when the sample detection device is powered on each day;

[0066] when the sample detection device is powered off each day;

[0067] when the sample detection device enters hibernation;

[0068] when the sample detection device exits hibernation;

[0069] a preset periodic cleaning time point;

[0070] receiving a deep cleaning start command.

[0071] In one embodiment, the temporary cleaning mode includes:

[0072] controlling addition of one or more cleaning liquids to the reaction cell and soaking the reaction cell for a preset time before discharging as waste liquid; and / or,

[0073] controlling flushing of the reaction cell with the washing liquid or the cleaning liquid.

[0074] In one embodiment, the cleaning liquid includes one or more hemolytic agents.

[0075] In one embodiment, the preset temporary cleaning condition includes any one or more of the following:

[0076] determining whether the cumulative number of tests of detection of a specific protein content in the reaction cell since the last time the deep cleaning mode or the temporary cleaning mode is performed on the reaction cell reaches a set number, and if so, controlling execution of the temporary cleaning mode to clean the reaction cell;

[0077] determining whether the cumulative number of tests of a specified measurement mode in the reaction cell since the last time the deep cleaning mode or the temporary cleaning mode is performed on the reaction cell reaches a set number, and if so, controlling execution of the temporary cleaning mode to clean the reaction cell;

[0078] determining whether the cumulative value of the detection result of a specific protein content in the reaction cell since the last time the deep cleaning mode or the temporary cleaning mode is performed on the reaction cell reaches a set value, and if so, controlling execution of the temporary cleaning mode to clean the reaction cell;

[0079] determining whether the number of samples with a result greater than a set value in a sample of a specific protein content test performed on the reaction cell after a last time a deep cleaning mode or a temporary cleaning mode is performed on the reaction cell reaches a set number, and if so, controlling to perform a temporary cleaning mode to clean the reaction cell;

[0080] acquiring a blank voltage of the reaction cell, and when the blank voltage is within a preset range, controlling to perform a temporary cleaning mode to clean the reaction cell; wherein the blank voltage is a voltage converted from a light sensed by controlling to irradiate a specific liquid in the reaction cell, for example, for a blank voltage acquired by using a scattering principle, the preset range is greater than a set voltage, and for a blank voltage acquired by using a transmission principle, the preset range is less than a set voltage.

[0081] In an embodiment, the specific liquid is a cleaning liquid or a cleaning solution; and the blank voltage is acquired after controlling to perform a regular cleaning mode to clean the reaction cell.

[0082] In an embodiment, a time consumption of one regular cleaning mode is less than a time consumption of one temporary cleaning mode, and the time consumption of one temporary cleaning mode is less than or equal to a time consumption of one deep cleaning mode.

[0083] According to a fourth aspect, an embodiment provides a computer-readable storage medium comprising a program, the program being executable by a processor to implement the method according to any of the embodiments herein.

[0084] According to a Xth aspect, an embodiment provides a computer-readable storage medium comprising a program, the program being executable by a processor to implement the method according to any of the embodiments herein

[0085] According to the sample detection device, the method and the computer-readable storage medium of the above-mentioned embodiments, three cleaning modes, i.e., a regular cleaning mode, a temporary cleaning mode and a deep cleaning mode, are introduced, which can make the reaction cell maintain a cleaning state for a long time. BRIEF DESCRIPTION OF DRAWINGS

[0086] Figure 1 a structural schematic diagram of a sample detection device according to an embodiment;

[0087] Figure 2 a structural schematic diagram of a specific protein detection component according to an embodiment;

[0088] Figure 3 a structural schematic diagram of a cleaning assembly according to an embodiment;

[0089] Figure 4A schematic view of the first cleaning port being eccentrically arranged in one embodiment;

[0090] Figure 5 A schematic view of the first cleaning port being eccentrically arranged in another embodiment;

[0091] Figure 6 A schematic view of the cleaning liquid supply part further comprising a recovery channel in one embodiment;

[0092] Figure 7 A schematic view of the structure of the cleaning assembly in another embodiment;

[0093] Figure 8 A schematic view of the structure of the cleaning assembly in yet another embodiment;

[0094] Figure 9 A flow chart of the method of the sample detection apparatus in one embodiment;

[0095] Figure 10 A flow chart of the method of the sample detection apparatus in another embodiment. DETAILED DESCRIPTION

[0096] The application will be further described with reference to the drawings in which like elements are referred to by like reference numerals. In the following description, numerous specific details are described to provide a thorough understanding of the application. However, one skilled in the relevant art will recognize that the application can be practiced without one or more of the specific details, or with other elements, materials, methods, components, materials, and / or the like. In some cases, well-known structures, materials, or acts are not shown or described in detail in order to avoid obscuring aspects of the application.

[0097] Furthermore, features described in the specification, examples, or claims can be combined in any manner, unless the combination of features is expressly prohibited by the claims. Also, the steps in a process can be rearranged or reordered in other embodiments without departing from the scope of the application. Therefore, the specification and drawings are to be regarded as illustrative only and not as restrictive.

[0098] The serial numbers of the components in the specification, such as "first", "second", etc., are used only to distinguish the described objects, and do not have any sequential or technical meaning. The "connection" or "coupling" in the specification includes direct and indirect connections (couplings) unless otherwise specified.

[0099] But research found that the protein generated in the process of detecting specific protein has strong adhesion ability, and there is no way to clean all the protein on the pool wall without general flushing; and in the case of long-term test accumulation, the pool wall will have more and more protein adhesion, so that the light transmittance of the pool body will continue to decrease, which will affect the normal test. In addition, protein adhesion also has a certain influence on the carryover pollution of normal measurement, which leads to deviation of measurement results.

[0100] In view of the above situation, three cleaning modes are introduced in the present application, namely, the conventional cleaning mode, the temporary cleaning mode and the deep cleaning mode, so that the reaction pool can be kept in good condition for a long time. The following will be described in detail.

[0101] Some embodiments provide a sample detection device which can perform blood routine detection and specific protein detection, for example, the sample detection device can detect blood routine items and specific protein content through the same blood sample. Please refer to Figure 1 The sample detection device in some embodiments includes a sample mechanism 10, a reagent mechanism 20, a blood routine component 30, a reaction pool 40, a specific protein detection component 50, a cleaning assembly 60 and a controller 70, which will be described in detail below.

[0102] The sample mechanism 10 is used to provide a sample to be tested. The sample mechanism 10 has various implementations. In some examples, the sample mechanism 10 can include a sample delivery module (SDM), a front-end track and a sample needle capable of being driven to move in two or three dimensions. The sample delivery module and the front-end track cooperate to supply a sample tube containing a sample and transport the sample tube containing the sample to a position such as a sample suction site. The sample needle can suck the sample from the sample tube at the sample suction site and discharge it to a corresponding position, such as the reaction pool 40 described above. In other examples, the sample mechanism 10 can also include a sample disc and a sample needle capable of being driven to move in two or three dimensions. The sample disc includes a plurality of sample sites such as sample tubes. The sample disc can dispatch the sample to a corresponding position such as a sample suction site by rotating its disc structure. The sample needle can suck the sample from the sample tube at the sample suction site and discharge it to a corresponding position, such as the reaction pool 40 described above.

[0103] The reagent mechanism 20 is used to provide reagents for detection. In some examples, the reagent mechanism 20 can be a reagent disc equipped with a reagent needle. The reagent disc has a disc structure and has a plurality of positions for carrying reagent containers. The reagent disc can rotate and drive the reagent containers it carries to rotate, so as to rotate the reagent containers to a specific position, such as a position for the reagent needle to suck reagents. The reagent mechanism 20 in the present application is used to provide reagents for specific protein detection and reagents for blood routine detection, wherein the reagents for blood routine detection include one or more hemolytic agents.

[0104] The blood routine component 30 is configured to process the sample provided by the sample mechanism with the reagent provided by the reagent mechanism 20 for blood routine test, so as to perform cell classification and / or counting on the sample. For example, the blood routine component 30 can include at least one detection module of a WBC (white blood cell) classification module, a WBC / HGB module and an RBC / PLT module. The WBC classification module is configured to obtain a five-classification result of WBC of the blood sample to be tested, the WBC / HGB module is configured to complete WBC counting and measurement of morphological parameters, and has the function of measuring HGB (hemoglobin), and the RBC / PLT module is configured to complete RBC (red blood cell) counting, PLT (blood platelet) counting and measurement of morphological parameters.

[0105] It can be seen that, through cooperation of the sample mechanism 10, the reagent mechanism 20 and the blood routine component 30, the blood routine test on the blood sample can be completed.

[0106] The reaction pool 40 is configured to receive the sample to be tested provided by the sample mechanism 10 and the reagent for specific protein detection such as latex reagent provided by the reagent mechanism 20, so that the two react to form a mixed sample liquid. It can be understood that, when the blood sample to be tested is serum or plasma without blood cells, the mixed sample liquid for specific protein detection can be prepared by reaction of the reagent for specific protein detection such as latex reagent and the blood sample; when the blood sample to be tested is whole blood sample containing blood cells, a hemolytic agent needs to be added to the whole blood sample first, and then the reagent for specific protein detection such as latex reagent is added after the blood cells are dissolved by reaction, so as to prepare the mixed sample liquid for specific protein detection. Of course, it can be understood by those skilled in the art that in some cases, it can also be necessary to add reagents such as buffer and / or diluent.

[0107] Please refer to Figure 2 The specific protein detection component 50 includes a detection area 51 made of a light-transmitting material, a light source 52 and a receiver 53 arranged correspondingly to the detection area 51. The light source 52 is configured to irradiate the mixed sample liquid flowing through the detection area 51, so that the receiver 53 can sense a light signal related to the content of specific protein in the mixed sample liquid. The light signal is used for detecting the content of specific protein in the mixed sample liquid. The receiver 53 can convert the light signal into a corresponding electrical signal, and the content of specific protein in the mixed sample liquid can be obtained after processing and analysis of the electrical signal.

[0108] ​​​​​​​​​​The washing assembly 60 is used to wash the reaction cell 40. In some examples, the washing assembly 60 can also be used to wash the sample mechanism 10, the reagent mechanism 20, the blood routine component 30, etc. This description mainly focuses on how the washing assembly 60 washes the reaction cell 40.

[0109] In some examples, the washing assembly 60 has three washing modes: a normal washing mode, a temporary washing mode, and a deep washing mode. The three washing modes are described as follows.

[0110] The normal washing mode is to wash the reaction cell 40 after each sample test is completed. In other words, after the sample solution mixed in the reaction cell 40 is detected, the washing assembly 60 performs the normal washing mode to wash the reaction cell 40, so that the reaction cell 40 can be restored to a measurement-ready state, and the detection result of the next sample is not affected, so that the reaction cell 40 can be used to detect the specific protein content of the next sample. The normal washing mode can be that the washing assembly 60 uses a washing liquid to flush the reaction cell. The washing liquid can be a dilution liquid, etc. Of course, the normal washing mode can also be combined with a cleaning liquid, for example, the reaction cell 40 is soaked with the cleaning liquid to wash the reaction cell 40. The cleaning liquid can be a hemolytic agent. Generally, through the normal washing mode, although most of the contaminants can be washed away, there may still be a small amount of contaminants adhering to the wall of the reaction cell 40, so that after a long time of use, there will be a cumulative effect, so that the contaminants in the reaction cell 40 can affect the accuracy of the detection result, or even cause irreversible damage to the reaction cell 40, for example, through long-term soaking of the cleaning liquid, or even manual scraping, the contaminants adhering to the wall of the reaction cell 40 cannot be removed. Considering this situation, the application also provides the temporary washing mode and the deep washing mode.

[0111] The deep washing mode is generally used at some special occasions, for example, when the machine is turned on or turned off, before the test starts on the same day, or after the test ends on the same day, to wash the reaction cell 40 more deeply. The deep washing mode can be that the washing assembly 60 uses one or more cleaning liquids to flush and / or soak the reaction cell 40 for a relatively long time, for example, at least 10 minutes, such as 30 minutes, so that the state of the reaction cell 40 is initialized and the reaction cell 40 is washed more thoroughly. The cleaning liquid involved in the deep washing mode can be a hemolytic agent. The deep washing mode can be performed once or twice a day.

[0112] The temporary cleaning mode is generally performed during the test on the same day. The sample testing device is triggered by the preset temporary cleaning condition, so that the cleaning assembly 60 performs an additional cleaning of the reaction pool 40 based on the regular cleaning mode. The specific cleaning method used in the temporary cleaning mode can be similar to the regular cleaning mode or similar to the deep cleaning mode. However, since the temporary cleaning mode is an additional cleaning during the test, in order to avoid affecting the normal test, the temporary cleaning mode can be shortened in time, for example, the temporary cleaning mode can be similar to the deep cleaning mode, but compared with the deep cleaning mode, it can reduce the cleaning time, reduce the number of suction and discharge during cleaning, reduce the types of cleaning fluid used, and so on. Taking the cleaning time as an example, the deep cleaning mode can be 10 minutes, and the temporary cleaning mode can be about 1 minute.

[0113] The above is some description of the three cleaning modes. During the cleaning process, the cleaning assembly 60 uses cleaning fluid, such as cleaning fluid, e.g. hemolytic agent, soaking, and waste liquid, and the like. The following describes how to achieve it in combination with related structures.

[0114] In some embodiments, please refer to Figure 3 The reaction pool 40 is provided with a waste liquid port 40a and a first cleaning port 40b. The waste liquid port 40a is used for discharging waste liquid, such as mixed sample liquid after detection, e.g. waste liquid generated by cleaning, and the like. Specifically, a waste liquid channel 41a and a waste liquid driving component 41 can be introduced; one end of the waste liquid channel 41a is connected with the waste liquid port 40a of the reaction pool 40, and the other end of the waste liquid channel 41a is connected with the waste liquid driving component 41; the waste liquid driving component 41 is used to discharge the liquid in the reaction pool 40 through the waste liquid channel 41a, for example, the waste liquid driving component 41 can include a pressure source to provide negative pressure, so that the waste liquid in the reaction pool 40 can flow into the waste liquid channel 41a through the waste liquid port 40a and be discharged. In some examples, the waste liquid port 40a or the waste liquid channel 41a can be controllably opened and closed, for example, by an electromagnetic valve. In some examples, the waste liquid port 40a is arranged at the bottom of the reaction pool 40.

[0115] The cleaning assembly 60 is capable of flushing the reaction cell with cleaning liquid. In some embodiments, the cleaning assembly 60 comprises a cleaning liquid supply component 61. The cleaning liquid supply component 61 comprises a cleaning liquid supply channel 61a connected to the first cleaning port 40b of the reaction cell 40, and is configured to supply cleaning liquid to the reaction cell 40 through the cleaning liquid supply channel 61a. In some examples, the first cleaning port 40b is provided on the sidewall of the reaction cell 40. In a specific cleaning process, the cleaning liquid supply component 61 supplies cleaning liquid into the reaction cell 40 through the first cleaning port 40b by, for example, positive pressure, to flush the sidewall of the reaction cell 40 and the like, and then the waste liquid is discharged through the waste liquid port 40a. The flushing action can be repeated several times to achieve better flushing effect.

[0116] In some embodiments, the first cleaning port 40b is arranged eccentrically, so that the cleaning liquid supplied by the cleaning liquid supply component 61 enters the reaction cell 40 from the first cleaning port 40b in an eccentric direction, so that the cleaning liquid forms a rotational flow state when entering the reaction cell 40. The inventors have found that the rotational flow state can enhance the flushing effect on the sidewall of the reaction cell 40, thereby achieving better cleaning effect. For example Figure 4 is an example of a top view of the reaction cell 40, in which the long diameter direction of the first cleaning port 40b is eccentric, i.e. not aligned with the center of the cross section of the reaction cell. In some embodiments, the first cleaning port 40b is arranged along the tangent direction of the sidewall of the reaction cell 40, i.e. the sidewall of the reaction cell 40, so that the rotational flow state formed is better. For example Figure 5This is an example. In the cyclone scheme, the higher the flow rate (speed) of the cleaning liquid added, the better the cleaning effect. Further, the inventors have found that the longer the cyclone lasts, the better the cleaning effect. However, after the cleaning liquid is added to the reaction tank 40, the cyclone gradually slows down due to capacity dissipation. If the liquid is continuously added to maintain the cyclone at this time, the liquid can overflow due to the limited volume of the tank. Therefore, the cleaning can be enhanced in the following two ways. First, the emptying action is added at the same time as the cleaning liquid is added, so that a continuous cyclone can be generated in the tank without causing overflow. Understandably, the emptying action here refers to the action of discharging waste liquid through the waste liquid discharge port 40a. Alternatively, a positive and negative pressure switching device can be introduced into the cleaning liquid supply member 61, i.e., the cleaning liquid supply member 61 can provide positive pressure or negative pressure. The cleaning liquid is added to the reaction tank 40 through the first cleaning port 40b via the cleaning liquid supply channel 61a to form a cyclone for cleaning. When the cyclone slows down, the liquid in the tank is sucked back into the cleaning liquid supply channel 61a by negative pressure, and then the liquid is re-added to the reaction tank 40 through, for example, the first cleaning port 40b by positive pressure to form a cyclone again. This suction and discharge method can form a continuous cyclone feature in the tank to enhance cleaning. The number of suction and discharge times can be one or more. Therefore, in some specific embodiments, please refer to Figure 6 The cleaning liquid supply member 61 further comprises a recovery channel 61b and a positive and negative pressure device 62. One end of the recovery channel 61b is connected to the waste liquid discharge port 40a, and the other end is connected to the positive and negative pressure device 62. When the positive and negative pressure device 62 works in a negative pressure state, it sucks waste liquid from the waste liquid discharge port 40a into the recovery channel 61b. The positive and negative pressure device 62 is then converted to a positive pressure to push the waste liquid in the recovery channel 61b back into the reaction tank 40, so as to recycle the discharged cleaning liquid as cleaning liquid for flushing the reaction tank 40.

[0117] The above describes how the cleaning assembly 60 provides cleaning liquid and cleans the reaction tank 40 with the cleaning liquid. The following describes how the cleaning assembly 60 provides cleaning liquid and cleans and / or soaks the reaction tank 40 with the cleaning liquid.

[0118] The cleaning assembly 60 can flush and / or soak the reaction tank with cleaning liquid. The cleaning liquid can be a hemolytic agent. In some embodiments, please refer to Figure 7The cleaning assembly 60 comprises a cleaning liquid supply component 65. Specifically, the reaction cell 40 can be provided with a second cleaning port 40c; the cleaning liquid supply component 65 comprises a cleaning liquid supply channel 65a connected with the second cleaning port 40c of the reaction cell 40, and the cleaning liquid supply component 65 is configured to supply cleaning liquid to the reaction cell 40 through the cleaning liquid supply channel 65a. The second cleaning port 40c can be provided in a similar manner as the first cleaning port 40b, for example, eccentrically, and further, for example, along a tangent direction of the reaction cell wall, i.e., the cell wall of the reaction cell 40, which will not be described here again. The first cleaning port 40b and the second cleaning port 40b can be the same port, or two different ports. Figure 7 For the case that the first cleaning port 40b and the second cleaning port 40b are two different ports, Figure 8 For the case that the first cleaning port 40b and the second cleaning port 40b are the same port. When the cleaning liquid supply component 65 is used to flush the reaction cell 40 with cleaning liquid, it can be similar to the action of the cleaning liquid supply component 61 used to flush the reaction cell 40 with cleaning liquid, for example, while the cleaning liquid is added, an emptying action is added so that a continuous vortex can be generated in the cell without causing overflow; for another example, a positive and negative pressure switching device can also be introduced into the cleaning liquid supply component 65, i.e., the cleaning liquid supply component 61 can provide positive pressure or negative pressure; after the cleaning liquid is added to the reaction cell 40 through the second cleaning port 40c via the cleaning liquid supply channel 65a, a vortex is formed for cleaning, when the vortex slows down, the liquid in the cell is sucked back into the cleaning liquid supply channel 65a by negative pressure, and then the liquid is re-added to the reaction cell 40 through the second cleaning port 40c, for example, by positive pressure, to form a vortex again; this suction and discharge mode can form a continuous vortex feature in the cell to enhance cleaning; the number of suction and discharge can be one or more times.

[0119] The above are some examples of providing cleaning liquid through the liquid path structure connected with the reaction cell 40. As described above, the cleaning liquid can be a hemolytic agent, and the detection of blood routine in the present application requires the use of a hemolytic agent to process a blood sample, so the reagent mechanism can also provide a hemolytic agent. Therefore, in some components, the cleaning liquid supply component 65 is multiplexed with the reagent mechanism 20 to provide one or more hemolytic agents as cleaning liquid to the reaction cell 40 during cleaning.

[0120] The above describes how the cleaning assembly 60 uses cleaning liquid and cleaning liquid to clean the reaction cell 40, and the following further describes how cleaning liquid and cleaning liquid are used in various cleaning modes in combination with three cleaning modes.

[0121] (I) Conventional cleaning mode

[0122] After each mixed sample solution in the reaction cell is detected, the controller 70 controls the cleaning assembly 60 to perform a regular cleaning mode to clean the reaction cell 40, so that the reaction cell 40 can detect the specific protein content of the next sample. Specifically, the detected mixed sample solution can be first emptied through the waste liquid channel 41a, or in the process of emptying the mixed sample solution, the cleaning liquid supply component 61 adds cleaning liquid to the reaction cell 40 through the first cleaning port 40b to flush the reaction cell 40, and the waste liquid is discharged through the waste liquid discharge port 40a; the flushing action can be repeated several times to achieve better flushing effect. In the example in which the first cleaning port 40b is eccentrically arranged, the cleaning liquid added to the reaction cell 40 by the cleaning liquid supply component 61 can form a cyclone state, and the emptying action is increased at the same time as the cleaning liquid is added, so that a continuous cyclone can be generated in the cell without causing overflow. In the example with the recovery channel 61b, the cleaning liquid is added to the reaction cell 40 through the first cleaning port 40b by the cleaning liquid supply channel 61a to form a cyclone for cleaning, and when the cyclone slows down, the liquid in the cell is sucked back into the cleaning liquid supply channel 61a by negative pressure through the recovery channel 61b, and then the liquid is added to the reaction cell 40 again through the first cleaning port 40b by positive pressure to form a cyclone again. The number of suction and discharge times can be one or more.

[0123] Under the premise of flushing with cleaning liquid, the cleaning liquid supply component 65 can add cleaning liquid to the reaction cell 40 for soaking for a predetermined time, for example, 5 seconds; by reacting the cleaning liquid with the pollutants adhering to the wall of the cell, the pollutants are decomposed and detached, achieving the effect of strengthening cleaning.

[0124] The cleaning liquid flushing and the cleaning liquid soaking can be alternately performed one or more times, for example, first performing cleaning liquid flushing, then performing cleaning liquid soaking, and then performing cleaning liquid flushing again; for another example, first performing cleaning liquid soaking, then performing cleaning liquid flushing, and then performing cleaning liquid soaking again. Generally, the last cleaning is cleaning liquid flushing.

[0125] (II) Deep cleaning mode

[0126] When a predetermined periodic cleaning opportunity is reached, the controller 70 controls the cleaning assembly 60 to perform a deep cleaning mode to clean the reaction cell 40. The predetermined periodic cleaning opportunity includes any one or more of the following: when the sample detection device is started every day, when the sample detection device is turned off every day, when the sample detection device enters hibernation, when the sample detection device exits hibernation, a predetermined periodic cleaning time point, or when a deep cleaning start command is received.

[0127] As can be seen, the regular cleaning mode is set in the course of the test procedure and is part of the test procedure, while the deep cleaning mode generally interrupts or breaks the test procedure, or the deep cleaning mode is performed before the test procedure has started or after the test procedure has ended. Since the regular cleaning mode is set in the course of the test procedure, and the test procedure of the sample testing device has certain requirements for speed and reagent consumption, the regular cleaning mode is subject to certain limitations, for example, it requires less time consumption, etc. As mentioned above, this can result in incomplete cleaning, so that a small amount of contaminants still continue to remain and adhere to the reaction cell 40. The deep cleaning mode is a more powerful cleaning than the regular cleaning mode. The deep cleaning mode is preferably arranged at a non-busy time, for example, the above-mentioned occasions of the sample testing device starting up every day, the sample testing device shutting down every day, the sample testing device entering hibernation, or the sample testing device exiting hibernation, etc. Through the use of strong cleaning liquid and appropriate increase in cleaning time, etc., a more thorough cleaning is performed, so that the reaction cell 40 can be restored to the initial state.

[0128] In some embodiments, the cleaning liquid supply component 65 can sequentially add one or more cleaning liquids to the reaction cell 40 and soak the reaction cell 40 for a preset time before being discharged as waste liquid. The preset time is, for example, 10 minutes or more. In the case of adding one cleaning liquid, the cleaning liquid supply component 65 first adds the cleaning liquid to the reaction cell 40 for soaking for a preset time, and then discharges it as waste liquid. In the case of adding multiple cleaning liquids, the cleaning liquid supply component 65 first adds the first cleaning liquid to the reaction cell 40 for soaking for a preset time, and then discharges it as waste liquid. The cleaning liquid supply component 65 then adds the second cleaning liquid to the reaction cell 40 for soaking for a preset time, and then discharges it as waste liquid. Through the reaction of the cleaning liquid with the contaminants adhering to the cell wall, the contaminants are decomposed and detached, achieving the effect of enhanced cleaning. The cleaning liquid can be different types of lysing agents.

[0129] In some embodiments, the cleaning liquid supply component 65 can also add one or more cleaning liquids to the reaction cell 40 to flush the reaction cell 40 with the cleaning liquid.

[0130] Therefore, the cleaning liquid supply component 65 can provide one or more cleaning liquids to alternately clean and soak in sequence. In the deep cleaning mode, on the basis of the cleaning liquid supply component 65 using the cleaning liquid to clean the reaction cell 40, the cleaning liquid supply component 61 can also add cleaning liquid to the reaction cell 40 for flushing. Further, the cleaning liquid flushing and the cleaning liquid soaking can be alternately performed one or more times, for example, first performing cleaning liquid flushing, then performing cleaning liquid soaking, and then performing cleaning liquid flushing again. For example, first performing cleaning liquid soaking, then performing cleaning liquid flushing. Generally, the last cleaning is cleaning liquid flushing.

[0131] (III) Temporary cleaning mode

[0132] The temporary cleaning mode is an additional cleaning during the testing process, which is triggered when certain conditions are met, and can be used as a supplement to the regular cleaning mode. Therefore, in some embodiments, when the preset temporary cleaning condition is reached, the controller 70 controls the cleaning assembly 60 to perform the temporary cleaning mode to clean the reaction cell 40. The preset temporary cleaning condition is generally a supplement to the preset periodic cleaning opportunity of the deep cleaning mode, and the condition for the device to determine that an additional temporary cleaning is needed is met when the sample testing device has not reached the preset periodic cleaning opportunity.

[0133] In some embodiments, the preset temporary cleaning condition includes one or more of the following:

[0134] (1) Whether the number of tests for detecting a specific protein reaction in the reaction cell 40 reaches a set number, and if so, performing a temporary cleaning of the reaction cell 40. Specifically, the controller 70 determines whether the cumulative number of tests for detecting the content of a specific protein in the reaction cell 40 since the last time the deep cleaning mode / temporary cleaning mode was performed on the reaction cell 40 reaches a set number, and if so, controls the cleaning assembly 60 to perform the temporary cleaning mode to clean the reaction cell 40.

[0135] (2) Whether the number of specified measurement modes performed reaches a set number, and if so, performing a temporary cleaning of the reaction cell 40. Specifically, the controller 70 determines whether the cumulative number of specified measurement modes performed in the reaction cell 40 since the last time the deep cleaning mode / temporary cleaning mode was performed on the reaction cell 40 reaches a set number, and if so, controls the cleaning assembly 60 to perform the temporary cleaning mode to clean the reaction cell 40. Condition (2) is similar to condition (1).

[0136] (3) Whether the cumulative content of a specific protein in the test results of the measurement performed in the reaction cell 40 reaches a set value, and if so, performing a temporary cleaning of the reaction cell 40. Specifically, the controller 70 determines whether the cumulative value of the content of a specific protein in the test results of the measurement performed in the reaction cell 40 since the last time the deep cleaning mode / temporary cleaning mode was performed on the reaction cell 40 reaches a set value, and if so, controls the cleaning assembly 60 to perform the temporary cleaning mode to clean the reaction cell 40.

[0137] (4) Each sample is measured in the reaction cell 40 to obtain a detection result of the specific protein content, and then the number of samples whose specific protein content in the detection result is greater than a set value is counted. If the number is large, the reaction cell 40 is subjected to a temporary cleaning. Specifically, the controller 70 determines whether the number of samples whose specific protein content in the detection result is greater than a set value reaches a set number among the samples subjected to the specific protein content detection in the reaction cell 40 after the last execution of the deep cleaning mode / temporary cleaning mode on the reaction cell 40, and controls the cleaning assembly 60 to perform the temporary cleaning mode to clean the reaction cell 40 if the number reaches the set number.

[0138] (5) The signal at a specified time point in the measurement process can also be collected as a judgment condition. For example, the blank voltage (the signal detection voltage value when the reaction cell is filled with a specific liquid, generally a diluent) is collected as a judgment condition. The blank voltage value is read each time a sample is tested. When the blank voltage satisfies a set condition, it indicates that the degree of contamination of the reaction cell has reached a certain level, and continued use can have a certain impact on the measurement value. At this time, the reaction cell 40 can be subjected to a temporary cleaning. Therefore, in some embodiments, the controller 70 acquires the blank voltage of the reaction cell, and controls the cleaning assembly 60 to perform the temporary cleaning mode to clean the reaction cell 40 when the blank voltage is within a preset range. The blank voltage is the voltage converted by the controller 70 by controlling the irradiation of the specific liquid in the reaction cell 40, the sensing of the transmitted or scattered light, and the conversion of the sensed light into an electrical signal. For the blank voltage collected by using the scattering principle, the preset range is greater than a set voltage. For the blank voltage collected by using the transmission principle, the preset range is less than a set voltage. In some embodiments, the specific liquid is a cleaning liquid or a cleaning solution. The blank voltage is collected after the controller 70 controls the cleaning assembly 60 to perform the regular cleaning mode to clean the reaction cell 40.

[0139] In the temporary cleaning mode, the cleaning process can be similar to the regular cleaning mode or the deep cleaning mode. In some embodiments, in the temporary cleaning mode, the cleaning solution supply component 65 adds one or more cleaning solutions to the reaction cell and soaks the reaction cell 40 for a preset time before discharging the cleaning solution as waste. And / or, the cleaning liquid supply component 61 uses the cleaning liquid or the cleaning solution to flush the reaction cell 40. Further, the cleaning liquid flushing and the cleaning solution soaking can be alternately performed one or more times. For example, the cleaning liquid flushing is performed first, then the cleaning solution soaking is performed, and then the cleaning liquid flushing is performed again. For another example, the cleaning solution soaking is performed first, and then the cleaning liquid flushing is performed. Generally, the last cleaning is the cleaning liquid flushing.

[0140] Since the temporary cleaning mode is a kind of cleaning that is "temporarily and additionally" added in the testing process, in some examples, the temporary cleaning mode can be a simplified version of the deep cleaning mode, for example, the temporary cleaning mode can reduce the cleaning time, reduce the number of suction and discharge times in the cleaning process, and reduce the types of cleaning liquids compared with the deep cleaning mode.

[0141] The above is some description of the three cleaning modes of the present application. In some examples, the time consumption of one regular cleaning mode is less than that of one temporary cleaning mode, and the time consumption of one temporary cleaning mode is less than or equal to that of one deep cleaning mode. For example, the execution of one regular cleaning mode takes about 10 seconds, the execution of one temporary cleaning mode takes about 1 minute, and the execution of one deep cleaning mode takes about 10 minutes.

[0142] The above is some description of the sample detection device of the present application.

[0143] In some examples, a method of a sample detection device is also disclosed. The sample detection device involved herein can be the sample detection device described in any of the embodiments of the present application. Please refer to Figure 9 In some examples, the method of the sample detection device comprises the following steps:

[0144] Step 100: Control to suck the sample to be tested and discharge the sample to be tested into the reaction pool for specific protein detection.

[0145] Step 110: Control to add latex reagent for reaction into the reaction pool for specific protein detection to prepare a mixed sample liquid.

[0146] Step 120: Control to irradiate the mixed sample liquid to detect the content of specific protein in the mixed sample liquid.

[0147] Steps 100 to 120 are the process of specific protein detection. It can be understood that when the blood sample to be tested is serum or plasma without blood cells, the mixed sample liquid for specific protein detection can be prepared by reacting the blood sample with reagents such as latex reagent, and then the specific protein detection can be performed; when the blood sample to be tested is whole blood sample containing blood cells, a hemolytic agent needs to be added to the whole blood sample first to dissolve the blood cells, and then a reagent for specific protein detection such as latex reagent is added to prepare the mixed sample liquid for specific protein detection. Of course, those skilled in the art can understand that in some examples, it can also be necessary to add reagents such as buffer and / or diluent.

[0148] Step 300: Control to perform cleaning on the reaction pool.

[0149] In some examples, the blood sample can also be used for both the blood routine test and the specific protein test, which will be described in detail below. Please refer to Figure 10 In some embodiments, the method of the sample testing device comprises the following steps:

[0150] Step 200: Control to draw the sample to be tested and discharge a portion of the sample to be tested to the blood routine reaction pool and the reaction pool for specific protein test respectively. For example, in some examples, after the sample needle draws the blood sample to be tested, a portion of the sample is discharged to the blood routine reaction pool, and the remaining sample is discharged to the reaction pool for specific protein test.

[0151] Step 210: Control to add one or more hemolytic agents to the blood routine reaction pool. This is to treat the sample to be tested with the hemolytic agent. The reagent for blood routine test includes one or more hemolytic agents.

[0152] Step 220: Control to perform the blood routine test on the sample to be tested treated with the hemolytic agent. For example, the cell classification and / or counting of the sample to be tested in step 220 can include the five classification of WBC, the counting of WBC and the measurement of morphological parameters, and can also include the measurement of HGB (hemoglobin), RBC (red blood cell), PLT (blood platelet) count and morphological parameters.

[0153] Step 230: Control to add the latex reagent for reaction to the reaction pool for specific protein test to prepare a mixed sample solution.

[0154] Step 240: Control to irradiate the mixed sample solution to detect the content of specific protein in the mixed sample solution.

[0155] In the above process, steps 200 to 220 complete the blood routine test on the sample. Steps 200, 230 and 240 complete the detection of specific protein.

[0156] Step 300: Control to perform cleaning on the reaction pool. Of course, in some examples, the blood routine reaction pool can also be cleaned. The process, method, mode and steps of cleaning the blood routine reaction pool can be similar to those of cleaning the reaction pool, which will not be described here.

[0157] The cleaning of the reaction pool in step 300 will be described in detail below.

[0158] In some embodiments, the cleaning of the reaction pool in step 300 includes three cleaning modes, namely the normal cleaning mode, the temporary cleaning mode and the deep cleaning mode, which will be described below.

[0159] (1) Regular cleaning mode

[0160] The regular cleaning mode is a regular cleaning of the reaction cell after each sample test is completed. In other words, each time after the mixed sample solution in the reaction cell is detected, the regular cleaning mode is controlled to be executed to clean the reaction cell, so as to restore the reaction cell to a measurement-ready state, avoid affecting the detection result of the next sample, and thus enable the reaction cell to detect the specific protein content of the next sample. The regular cleaning mode can be to control the use of cleaning liquid to flush the reaction cell, and the cleaning liquid can be dilution liquid, etc. Of course, the regular cleaning mode can also be combined with cleaning liquid, for example, adding cleaning liquid to the reaction to soak the reaction cell for cleaning, and the cleaning liquid can be hemolytic agent.

[0161] Therefore, in some embodiments, step 300 controls the reaction cell to perform cleaning, including: after each time the mixed sample solution in the reaction cell is detected, the regular cleaning mode is controlled to be executed to clean the reaction cell, so as to enable the reaction cell to detect the specific protein content of the next sample. In some embodiments, the regular cleaning mode includes: controlling the use of cleaning liquid to flush the reaction cell, and specifically, the cleaning liquid can be controlled to be added to the reaction cell in an eccentric direction, for example, the cleaning liquid can be controlled to be added to the reaction cell in a tangential direction along the wall of the reaction cell, so that the cleaning liquid forms a cyclone state when it is added to the reaction cell. Further, while controlling the use of cleaning liquid to flush the reaction cell, the cleaning liquid in the reaction cell is continuously discharged as waste liquid, or while controlling the use of cleaning liquid to flush the reaction cell, the cleaning liquid in the reaction cell is continuously discharged and recycled as cleaning liquid for flushing the reaction cell, so that the duration of the cyclone is relatively long and the cleaning effect is better. In some examples, under the premise of using cleaning liquid to flush, cleaning liquid such as LH hemolytic agent can also be controlled to be added to the reaction cell, and the reaction cell is soaked for a preset time and then discharged as waste liquid; through the reaction of the cleaning liquid with the pollutants adhering to the wall of the cell, the pollutants are decomposed and detached, so as to achieve the effect of strengthening cleaning. The cleaning liquid flushing and the cleaning liquid soaking can be alternately performed one or more times, for example, first performing cleaning liquid flushing, then performing cleaning liquid soaking, and then performing cleaning liquid flushing again; for another example, first performing cleaning liquid soaking, then performing cleaning liquid flushing. Generally, the last cleaning is cleaning liquid flushing.

[0162] Generally, through the regular cleaning mode, although most of the contaminants can be cleaned, there can still be a small amount of contaminants adhering to the walls of the reaction cell, thus after long time use, there can be a cumulative effect, so that the contaminants in the reaction cell can be sufficient to affect the accuracy of the test results, or even cause irreversible damage to the reaction cell, for example, through long time soaking in cleaning solution, or even manual scraping, the contaminants adhering to the walls of the reaction cell cannot be removed. In view of this situation, the present application is also provided with two cleaning modes, namely the temporary cleaning mode and the deep cleaning mode.

[0163] (ii) Deep cleaning mode

[0164] The deep cleaning mode is generally a relatively deep cleaning of the reaction cell 40 at some special occasions, such as at the occasion of turning on or off the machine, before the test starts on the same day, or after the test ends on the same day. The deep cleaning mode can be that the cleaning assembly 60 uses one or more cleaning solutions to flush and / or soak the reaction cell 40 for a relatively long time, for example, at least 10 minutes, such as 30 minutes, so that the state of the reaction cell 40 can be initialized and cleaned thoroughly. The cleaning solution involved in the deep cleaning mode can be a hemolytic agent. The deep cleaning mode can be performed once or twice every day at a fixed time.

[0165] Therefore, in some embodiments, the step 300 controls the reaction cell to perform a cleaning, including: when a preset periodic cleaning time is reached, controlling the reaction cell to perform a deep cleaning mode. The preset periodic cleaning time includes any one or more of the following: when the sample detection device is powered on each day, when the sample detection device is powered off each day, when the sample detection device enters hibernation, when the sample detection device exits hibernation, a preset periodic cleaning time point, or when a deep cleaning start command is received. In some embodiments, the deep cleaning mode includes: controlling the addition of one or more cleaning liquids to the reaction cell, and soaking the reaction cell for a preset time before being discharged as waste liquid. Further, the deep cleaning mode can also include: controlling the use of cleaning liquid or the cleaning liquid to flush the reaction cell. When the cleaning liquid or the cleaning liquid is used to flush the reaction cell in the deep cleaning mode, the conventional cleaning mode can be referred to, that is, by adding the cleaning liquid to the reaction cell along the eccentric direction, especially along the tangent direction of the reaction cell wall, so that the cleaning liquid forms a cyclone state when added to the reaction cell. Further, the deep cleaning mode also includes: controlling the use of cleaning liquid / the cleaning liquid to flush the reaction cell while continuously discharging the cleaning liquid / the cleaning liquid in the reaction cell as waste liquid, or controlling the use of cleaning liquid / the cleaning liquid to flush the reaction cell while continuously discharging the cleaning liquid / the cleaning liquid in the reaction cell and recycling it as cleaning liquid / cleaning liquid for flushing the reaction cell; in this way, the cleaning liquid forms a cyclone in the reaction cell for a longer duration, and the cleaning effect is better.

[0166] In the conventional cleaning mode, a cleaning liquid with strong cleaning ability can be used as a cleaning liquid, and in the deep cleaning mode, a cleaning liquid with strong cleaning ability can be used as a cleaning liquid.

[0167] In some examples, in the deep cleaning mode, cleaning liquid flushing and cleaning liquid soaking can be alternated one or more times, for example, first cleaning liquid flushing, then cleaning liquid soaking, and then cleaning liquid flushing; for example, first cleaning liquid soaking, then cleaning liquid flushing. Generally, the last cleaning is cleaning liquid flushing.

[0168] (Three) Temporary cleaning mode

[0169] The temporary cleaning mode is generally performed during the test on the same day. The sample detection device is triggered by the preset temporary cleaning condition to perform an additional cleaning of the reaction pool based on the regular cleaning mode. The specific cleaning method used in the temporary cleaning mode can be similar to the regular cleaning mode or similar to the deep cleaning mode. However, since the temporary cleaning mode is an additional cleaning during the test, in order to avoid affecting the normal test, the temporary cleaning mode can be shortened in time, for example, the temporary cleaning mode can be similar to the deep cleaning mode, but compared with the deep cleaning mode, it can reduce the cleaning time, reduce the number of suction and discharge during cleaning, reduce the types of cleaning liquid used, and so on. Taking the cleaning time as an example, the deep cleaning mode can be 10 minutes, and the temporary cleaning mode can be about 1 minute.

[0170] Therefore, in some embodiments, step 300 controls the reaction pool to perform cleaning, including: when the preset temporary cleaning condition is reached, controlling to perform a temporary cleaning mode to clean the reaction pool.

[0171] In some embodiments, the preset temporary cleaning condition includes one or more of the following:

[0172] (1) Whether the number of tests for detecting a specific protein reaction in the reaction pool reaches a set number, if so, performing a temporary cleaning of the reaction pool. Specifically, it is judged whether the cumulative number of tests for detecting the content of the specific protein in the reaction pool since the last deep cleaning mode / temporary cleaning mode performed on the reaction pool 40 reaches a set number, if so, controlling to perform a temporary cleaning mode to clean the reaction pool.

[0173] (2) Whether the specified measurement mode, generally referring to the measurement mode containing specific protein detection, reaches a set number, if so, performing a temporary cleaning of the reaction pool. Specifically, it is judged whether the cumulative number of specified measurement modes performed in the reaction pool since the last deep cleaning mode / temporary cleaning mode performed on the reaction pool reaches a set number, if so, controlling to perform a temporary cleaning mode to clean the reaction pool. Condition (2) is similar to condition (1).

[0174] (3) Whether the cumulative value of the specific protein content in the detection results of the measurement performed by each sample in the reaction pool reaches a set value, if so, performing a temporary cleaning of the reaction pool. Specifically, it is judged whether the cumulative value of the detection results of the specific protein content performed in the reaction pool since the last deep cleaning mode / temporary cleaning mode performed on the reaction pool reaches a set value, if so, controlling to perform a temporary cleaning mode to clean the reaction pool.

[0175] (4) Each sample is measured in the reaction cell to obtain a detection result of the specific protein content, and then the number of samples whose specific protein content in the detection result is greater than a set value is counted. If the number is large, the reaction cell is temporarily cleaned. Specifically, it is determined whether the number of samples whose specific protein content in the detection result is greater than a set value among the samples whose specific protein content is detected in the reaction cell after the last time the deep cleaning mode / temporary cleaning mode is performed on the reaction cell reaches a set number. If yes, the temporary cleaning mode is controlled to clean the reaction cell.

[0176] (5) In some examples, a signal at a specified time point during the measurement process can be collected as a judgment condition. For example, a blank voltage (a signal detection voltage value when the reaction cell is filled with a specific liquid, generally a diluent) can be collected as a judgment condition. The blank voltage value is read each time a sample is tested. When the blank voltage meets a set condition, it indicates that the degree of contamination of the reaction cell has reached a certain level, and continued use may have a certain impact on the measurement value. At this time, the reaction cell can be temporarily cleaned. Therefore, in some examples, the blank voltage of the reaction cell is obtained, and when the blank voltage is within a preset range, the temporary cleaning mode is controlled to clean the reaction cell. The blank voltage is a voltage converted from the transmitted or scattered light when the specific liquid in the reaction cell is irradiated. For example, for the blank voltage collected by using the scattering principle, the preset range is greater than a set voltage. For the blank voltage collected by using the transmission principle, the preset range is less than a set voltage. In some examples, the specific liquid is a cleaning liquid or a cleaning solution. The blank voltage is collected after the reaction cell is cleaned by the regular cleaning mode.

[0177] As described above, the cleaning process in the temporary cleaning mode can be similar to that in the regular cleaning mode or the deep cleaning mode. In some examples, in the temporary cleaning mode, one or more cleaning solutions are added to the reaction cell, and the reaction cell is soaked for a preset time and then drained as waste. In addition, the reaction cell can be flushed with a cleaning liquid or a cleaning solution. Further, the cleaning liquid flushing and the cleaning solution soaking can be alternated one or more times. For example, the cleaning liquid flushing is performed first, then the cleaning solution soaking is performed, and then the cleaning liquid flushing is performed again. For another example, the cleaning solution soaking is performed first, and then the cleaning liquid flushing is performed.

[0178] Since the temporary cleaning mode is an additional cleaning mode during the test process, in some examples, the temporary cleaning mode can be a simplified version of the deep cleaning mode. For example, the temporary cleaning mode can reduce the cleaning time, the number of suction and discharge times during the cleaning process, and the number of cleaning solutions compared with the deep cleaning mode.

[0179] The above is a brief description of the three cleaning modes of the present application. In some embodiments, the time required for a regular cleaning mode is less than that of a temporary cleaning mode, and the time required for a temporary cleaning mode is less than or equal to that of a deep cleaning mode. For example, the time required for a regular cleaning mode is about 10 seconds, the time required for a temporary cleaning mode is about 1 minute, and the time required for a deep cleaning mode is about 10 minutes.

[0180] One arrangement of the three cleaning modes can be as follows: after each detection of a mixed sample solution in the reaction cell, a regular cleaning mode is executed to clean the reaction cell so that the next sample can be detected for a specific protein content; after each detection of a mixed sample solution in the reaction cell, it is determined whether a preset periodic cleaning opportunity has been reached. If so, a deep cleaning mode is executed to clean the reaction cell. If not, it is further determined whether a preset temporary cleaning condition has been reached. If so, a temporary cleaning mode is executed to clean the reaction cell. If not, the sample detection device is in a ready state and can start the detection of a specific protein in the next sample.

[0181] The sample detection device and method of the present application can maintain the reaction cell in a clean state for a long time. Specifically, the sample detection device and method of the present application introduce a temporary cleaning mode and a deep cleaning mode in addition to a regular cleaning mode, which can thoroughly remove accumulated contaminants during use, restore the initial state of the cell body, and maintain the accuracy of the detection results. At the same time, the cell body can be maintained in a good state for a long time, so there is no need for manual periodic cleaning of the cell wall, etc. In addition, the sample detection device and method of the present application also prolong the service life of the reaction cell and reduce the cost of using the instrument. In the prior art, the reaction cell often needs to be replaced every 1-2 months because the cell body is often not thoroughly cleaned and is irreversibly contaminated, i.e., the contaminants on the cell wall cannot be removed even by manual cleaning, so the cell body must be replaced. In addition, the sample detection device and method of the present application introduce a temporary cleaning mode and a deep cleaning mode, especially a temporary cleaning mode, which can reduce the time required for cleaning during normal testing, such as the time required for a regular cleaning mode. For example, the cleaning time during normal testing can be reduced from 20-30 seconds to about 10 seconds, which improves the testing speed.

[0182] Various exemplary embodiments are described herein. However, it will be recognized by those skilled in the art that changes and modifications can be made to the exemplary embodiments without departing from the scope of the present disclosure. For example, various operational steps and components for carrying out the operational steps can be implemented in different sequences and / or omitted, combined, or combined in various ways than presented in the figures and / or descriptions without departing from the principles of the present disclosure.

[0183] In the above-described embodiments, all or part can be implemented by software, hardware, firmware, or any combination thereof. In addition, as understood by those skilled in the art, the principles herein can be reflected in a computer program product on a computer readable storage medium preloaded with computer readable program code. Any tangible, non-transitory computer readable storage medium can be used, including magnetic storage devices (hard disk, floppy disk, etc.), optical storage devices (CD-ROM, DVD, Blu Ray disc, etc.), flash memory, and / or the like. These computer program instructions can be loaded onto a general purpose computer, a special purpose computer, or other programmable data processing apparatus to produce a machine, so that these instructions executed on the computer or other programmable data processing apparatus can generate a device that implements the specified functions. These computer program instructions can also be stored in a computer readable memory that can instruct the computer or other programmable data processing apparatus to operate in a specific manner, so that the instructions stored in the computer readable memory can form a manufactured item that includes an implementation device that implements the specified functions. Computer program instructions can also be loaded onto a computer or other programmable data processing apparatus, so as to execute a series of operational steps on the computer or other programmable data processing apparatus to generate a computer-implemented process, so that the instructions executed on the computer or other programmable data processing apparatus can provide steps for implementing the specified functions.

[0184] Although the principles herein have been illustrated in various embodiments, many modifications in structure, arrangement, proportions, elements, materials, and components specially adapted to specific environments and operational requirements can be used without departing from the principles and scope of the present disclosure. The above modifications and other changes or modifications will be included within the scope of the principles herein.

[0185] The foregoing detailed description has been presented for purposes of illustration and description. However, various modifications and changes are possible in the implementation of the disclosure. Accordingly, the disclosure is intended to embrace all modifications and alterations within the scope and spirit of the disclosure. Thus, the scope of the disclosure is not intended to be limited to the particular form set forth herein, but includes all features that might be provided within the scope and spirit of the disclosure. Likewise, a variety of advantages and features have been set forth in the description herein with reference to the various embodiments. It is to be understood that not necessarily all advantages can be achieved in accordance with any particular embodiment. Further, solutions to problems can be appreciated by one skilled in the art upon reading the disclosure. Any feature, structure, material, or combination thereof described herein is meant to be illustrative and not restrictive. The scope of the disclosure is therefore intended to cover any variations, uses, or adaptations of the application including such departures from the present disclosure as come within known or customary practice in the art to which the disclosure pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting. The use of the terms "including", "comprising", or "having" and variations thereof herein is meant to encompass the items listed thereafter and equivalents thereof as well as additional items. Further, the use of the terms "coupled" and variations thereof is meant to encompass a direct connection between two elements and / or an indirect connection between two elements through one or more intervening elements.

[0186] Those skilled in the art will recognize that many modifications and variations of the described implementations can be made. It is therefore intended that the scope of the application be limited only by the breadth of the claims.

Claims

1. A sample testing device, characterized in that, include: A sample preparation facility, used to provide samples to be tested; A reagent facility for providing reagents for specific protein detection and reagents for routine blood tests, wherein the routine blood test reagents include one or more hemolytic agents; A routine blood test component is used to process a sample to be tested provided by a sample preparation unit using a routine blood test reagent provided by the reagent preparation unit, so as to perform cell classification and / or counting on the sample to be tested. The reaction chamber is used to receive the sample to be tested provided by the sample unit and the reagent for detecting a specific protein provided by the reagent unit, so that the two react to form a mixed sample solution; A specific protein detection component includes a detection area made of a light-transmitting material, a light source and a receiver disposed corresponding to the detection area, wherein the light source is used to illuminate the mixed sample liquid flowing through the detection area, so that the receiver senses a light signal related to the content of a specific protein in the mixed sample liquid, and the light signal is used to detect the content of the specific protein in the mixed sample liquid; A cleaning assembly is provided for cleaning the reaction tank. The cleaning assembly includes a cleaning solution supply unit that reuses the reagent mechanism to provide one or more hemolysing agents as cleaning solutions to the reaction tank during cleaning. The cleaning assembly has three cleaning modes: a regular cleaning mode, a temporary cleaning mode, and a deep cleaning mode, and at least one of these cleaning modes uses the one or more hemolysing agents as cleaning solutions to clean the reaction tank. The regular cleaning mode is configured during the testing process, and the deep cleaning mode is configured before the testing process begins or after the testing process has ended. The controller is used to control the cleaning assembly to clean the reaction tank, specifically including: After each sample solution is mixed in the reaction chamber and tested, the controller controls the cleaning assembly to perform a routine cleaning mode to clean the reaction chamber so that the reaction chamber can be used to detect the specific protein content of the next sample. When the preset temporary cleaning conditions are met, the controller controls the cleaning components to execute the temporary cleaning mode to clean the reaction tank. The temporary cleaning mode is used to clean the pollutants that remain and adhere to the tank wall after the regular cleaning mode and have accumulated through the cumulative effect. When the preset periodic cleaning time is reached, the controller controls the cleaning component to perform a deep cleaning mode to clean the reaction tank.

2. The sample detection device as described in claim 1, characterized in that, The reaction tank is equipped with a waste liquid discharge port and a first cleaning port; The sample detection device further includes a waste liquid channel and a waste liquid driving component; one end of the waste liquid channel is connected to the waste liquid discharge port of the reaction tank, and the other end is connected to the waste liquid driving component; the waste liquid driving component is used to discharge the liquid in the reaction tank through the waste liquid channel; The cleaning assembly further includes a cleaning fluid supply component; the cleaning fluid supply component includes a cleaning fluid supply channel connected to a first cleaning port of the reaction tank, and the cleaning fluid supply component is used to supply cleaning fluid to the reaction tank through the cleaning fluid supply channel; the cleaning fluid supply component is used to supply cleaning fluid to the reaction tank; The first cleaning port is eccentrically positioned so that the cleaning fluid supplied by the cleaning fluid supply component enters the reaction tank from the first cleaning port in an eccentric direction.

3. The sample detection device as described in claim 2, characterized in that, The first cleaning port is arranged along the tangential direction of the reaction tank wall.

4. The sample detection device as described in claim 2 or 3, characterized in that, The cleaning fluid supply component also includes a recycling channel, one end of which is connected to the waste liquid discharge port and the other end of which is connected to the first cleaning port. This channel is used to recycle the cleaning fluid discharged from the waste liquid discharge port and reuse it as cleaning fluid for rinsing the reaction tank.

5. The sample detection device as described in claim 2, characterized in that, The reaction tank is provided with a second cleaning port; the cleaning solution supply component includes a cleaning solution supply channel connected to the second cleaning port of the reaction tank, and the cleaning solution supply component is used to supply the cleaning solution to the reaction tank through the cleaning solution supply channel. The second cleaning port is eccentrically positioned so that the cleaning fluid supplied by the cleaning fluid supply unit enters the reaction tank from the second cleaning port in an eccentric direction.

6. The sample detection device as described in claim 5, characterized in that, The second cleaning port is arranged along the tangential direction of the reaction tank wall.

7. The sample detection device as described in claim 1, characterized in that, The time required for a regular cleaning cycle is less than the time required for a temporary cleaning cycle, and the time required for a temporary cleaning cycle is less than or equal to the time required for a deep cleaning cycle.

8. A method for using a sample testing device, the sample testing device comprising a reagent mechanism and a cleaning assembly; characterized in that, The method includes: Control the aspiration of the test sample and discharge a portion of the test sample into the blood routine reaction chamber and the reaction chamber for specific protein detection, respectively; The reagent mechanism is controlled to add one or more hemolytic agents to the blood routine reaction pool; Control the testing of at least one item in the complete blood count for test samples treated with hemolytic agents; The reagent mechanism is controlled to add latex reagents for reaction into the reaction chamber for the detection of a specific protein to prepare a mixed sample solution; The mixed sample solution is irradiated to detect the content of a specific protein in the mixed sample solution; The cleaning assembly controls the cleaning of the reaction tank, the cleaning assembly including a cleaning solution supply component that reuses the reagent mechanism to provide one or more hemolysing agents as the cleaning solution to the reaction tank during cleaning; the method includes three cleaning modes for the reaction tank: a regular cleaning mode, a temporary cleaning mode, and a deep cleaning mode, and at least one of the cleaning modes uses the one or more hemolysing agents as the cleaning solution to clean the reaction tank; the regular cleaning mode is set during the test process, and the deep cleaning mode is set before the test process begins or after the test process ends; controlling the cleaning of the reaction tank includes: After each controlled detection of the mixed sample solution in the reaction chamber, a routine cleaning mode is executed to clean the reaction chamber, so that the reaction chamber can be used to detect the specific protein content of the next sample. When preset temporary cleaning conditions are met, a temporary cleaning mode is executed to clean the reaction tank; the temporary cleaning mode is used to clean the contaminants that remain and adhere to the tank wall after the regular cleaning mode and have accumulated through cumulative effects. When the preset periodic cleaning time is reached, the deep cleaning mode is controlled to clean the reaction tank.

9. The method as described in claim 8, characterized in that, The conventional cleaning modes include: The reaction tank is rinsed with cleaning solution.

10. The method as described in claim 9, characterized in that, The control method involves rinsing the reaction tank with a cleaning solution, including: The cleaning solution is added to the reaction tank in an eccentric direction, where the eccentric direction is the tangential direction of the reaction tank wall.

11. The method as described in claim 10, characterized in that, The conventional cleaning modes also include: While controlling the use of cleaning fluid to rinse the reaction tank, the cleaning fluid in the reaction tank is also continuously discharged as waste liquid, or... While controlling the use of cleaning fluid to rinse the reaction tank, the cleaning fluid in the reaction tank is also continuously discharged and recycled for reuse as cleaning fluid for rinsing the reaction tank.

12. The method as described in claim 9, characterized in that, The conventional cleaning modes also include: The cleaning solution is added to the reaction tank and then discharged as waste liquid after the reaction tank has been soaked for a preset time.

13. The method as described in claim 12, characterized in that, The cleaning solution includes one or more hemolytic agents.

14. The method as described in claim 8, characterized in that, The deep cleaning mode includes: One or more cleaning solutions are added to the reaction tank and the tank is soaked for a preset time before being discharged as waste liquid.

15. The method as described in claim 8, characterized in that, The deep cleaning mode includes: controlling the addition of one or more cleaning solutions to the reaction tank, and immersing the reaction tank for a preset time before discharging it as waste liquid.

16. The method as described in claim 14, characterized in that, The deep cleaning mode also includes: The reaction tank is rinsed with a cleaning solution or the cleaning fluid.

17. The method as described in claim 15, characterized in that, The deep cleaning mode also includes: controlling the use of cleaning fluid or the cleaning solution to rinse the reaction tank.

18. The method as described in claim 16, characterized in that, The deep cleaning mode also includes: While controlling the use of cleaning solution / the cleaning fluid to rinse the reaction tank, the cleaning solution / the cleaning fluid in the reaction tank is also continuously discharged as waste liquid, or... While controlling the use of cleaning fluid / the cleaning solution to rinse the reaction tank, the cleaning fluid / the cleaning solution in the reaction tank is also continuously discharged and recycled as cleaning fluid / the cleaning solution for rinsing the reaction tank again.

19. The method as described in claim 17, characterized in that, The deep cleaning mode further includes: while controlling the use of cleaning fluid / the cleaning solution to rinse the reaction tank, continuously discharging the cleaning fluid / the cleaning solution in the reaction tank as waste liquid; or, while controlling the use of cleaning fluid / the cleaning solution to rinse the reaction tank, continuously discharging and recycling the cleaning fluid / the cleaning solution in the reaction tank as cleaning fluid / the cleaning solution to be used again to rinse the reaction tank.

20. The method as described in claim 8, 14, 15, 16, 17, 18 or 19, characterized in that, The preset periodic cleaning times include any one or more of the following: When the sample testing equipment is turned on each day; When the sample testing equipment is shut down daily; When the sample testing equipment enters sleep mode; When the sample testing equipment exits sleep mode; Preset scheduled cleaning times; A deep cleaning start command has been received.

21. The method as described in claim 8, characterized in that, The temporary cleaning modes include: Control the addition of one or more cleaning solutions to the reaction tank, and after soaking the reaction tank for a preset time, discharge it as waste liquid; and / or, The reaction tank is rinsed with cleaning fluid or detergent.

22. The method as described in claim 8, characterized in that, The temporary cleaning mode includes: controlling the addition of one or more cleaning solutions to the reaction tank and immersing the reaction tank for a preset time before discharging it as waste liquid; and / or controlling the use of cleaning solution or detergent to rinse the reaction tank.

23. The method as described in claim 14, 15, 16, 17, 18, 19, 21 or 22, characterized in that, The cleaning solution includes one or more hemolytic agents.

24. The method as described in claim 8, 21, or 22, characterized in that, The preset temporary cleaning conditions include any one or more of the following: Determine whether the cumulative number of tests for detecting the content of a specific protein in the reaction tank has reached a set number since the last deep cleaning mode or temporary cleaning mode was performed on the reaction tank. If it has, control the execution of the temporary cleaning mode to clean the reaction tank. Determine whether the cumulative number of specified measurement modes performed on the reaction tank since the last deep cleaning mode or temporary cleaning mode was performed has reached a set number. If it has, control the execution of the temporary cleaning mode to clean the reaction tank. Determine whether the cumulative value of the detection results of a specific protein content in the reaction tank since the last deep cleaning mode or temporary cleaning mode was performed has reached a set value. If it has, control the execution of the temporary cleaning mode to clean the reaction tank. If the number of samples with a specific protein content detection result greater than a set value in the reaction tank since the last deep cleaning mode or temporary cleaning mode was performed on the reaction tank has reached a set number, then the temporary cleaning mode is controlled to be executed to clean the reaction tank. The blank voltage of the reaction tank is obtained. When the blank voltage is within a preset range, a temporary cleaning mode is controlled to clean the reaction tank. The blank voltage is the voltage obtained by controlling the irradiation of the specific liquid in the reaction tank when the reaction tank is filled with a specific liquid, sensing transmitted or scattered light and converting it into voltage.

25. The method as described in claim 24, characterized in that, The specific liquid is a cleaning solution or a cleaning fluid; when the control executes a regular cleaning mode to clean the reaction tank, the control collects the blank voltage.

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