Electrochemical sensor and method for manufacturing the same

Abstract

The embodiment of the present application provides an electrochemical sensor and a manufacturing method thereof, which relates to the field of electrochemical analysis technology, including a substrate and an electrode arranged on the substrate; the electrode includes a power electrode and a plurality of working electrodes, the surfaces of the plurality of working electrodes respectively have a plurality of reagent enzymes, and the reagent enzymes on the surface of each working electrode are different; wherein the power electrode is located in the first area of ​​the substrate; the plurality of working electrodes are located in the second area of ​​the substrate; the second area includes a plurality of sub-areas, the plurality of sub-areas are independent of each other on the substrate, and the plurality of working electrodes are respectively located in different sub-areas; and further includes a guide layer, which is a paper material, the guide layer is located above the electrode, and the guide layer covers the first area and the second area. An electrochemical sensor and an electrochemical detector provided by the embodiment of the present application can reduce the situation of mutual interference between working electrodes and improve the accuracy of the detection results.

Description

Technical Field

[0001] The embodiments of the present application relate to the field of electrochemical analysis technology, and in particular, to an electrochemical sensor and a method for manufacturing the same. Background Art

[0002] Electrochemical enzyme sensors are enzyme biosensors, and their basic structural unit is composed of a substance recognition element (immobilized enzyme membrane) and a signal converter (substrate electrode). When an enzymatic reaction occurs on the enzyme membrane, the electroactive substances produced are responded to by the substrate electrode. The function of the substrate electrode is to convert chemical signals into electrical signals for detection.

[0003] At present, electrochemical sensors usually include multiple working electrodes, on which different specific enzymes are fixed respectively. Different specific enzymes can catalyze the corresponding analytes in the liquid to react and generate reactants to make the working electrodes work. Therefore, electrochemical sensors can detect multiple components in the liquid to be detected.

[0004] However, since the products of reactions of different analytes catalyzed by different specific enzymes may be the same, and all working electrodes are located on the same substrate, when the distances between different working electrodes are very close, mutual interference may occur between the different working electrodes, resulting in errors in the detection results of the components in the liquid. Summary of the invention

[0005] The embodiments of the present application provide an electrochemical sensor and a method for manufacturing the same, which are intended to reduce mutual interference between working electrodes and improve the accuracy of detection results.

[0006] A first aspect of an embodiment of the present application provides an electrochemical sensor, comprising a substrate and an electrode disposed on the substrate;

[0007] The electrodes include a power supply electrode and a plurality of working electrodes, the surfaces of the plurality of working electrodes respectively have a plurality of reagent enzymes, and the reagent enzymes on the surface of each of the working electrodes are different;

[0008] Wherein, the power electrode is located in the first area of ​​the substrate; the plurality of working electrodes are located in the second area of ​​the substrate;

[0009] The second region includes a plurality of sub-regions, the plurality of sub-regions are independent of each other on the substrate, and the plurality of working electrodes are respectively located in different sub-regions;

[0010] It also includes a guide layer, which is a paper material. The guide layer is located above the electrode, covers the first area and the second area, and one edge of the guide layer extends to the edge of the substrate.

[0011] Optionally, the material of the guide layer includes: filter paper, chromatography paper or blotting paper.

[0012] Optionally, the plurality of working electrodes are located on one side or both sides of the power electrode;

[0013] Alternatively, the plurality of working electrodes are evenly spaced apart along the circumference of the power electrode.

[0014] Optionally, the guide layer located in the second region has a width of 10 μm-5 mm.

[0015] Optionally, the power supply electrode includes: an active electrode.

[0016] Optionally, the power supply electrode includes: a counter electrode and a reference electrode.

[0017] A second aspect of an embodiment of the present application provides a method for manufacturing an electrochemical sensor, the method comprising: providing a substrate;

[0018] forming a power electrode and a plurality of working electrodes on a substrate by a printing process and drying the electrodes;

[0019] Covering the power electrode and the leads of the plurality of working electrodes with an insulating slurry and drying the insulating slurry;

[0020] Printing reagent enzymes on the plurality of working electrodes by a printing process and drying them;

[0021] A guide layer is covered on the power electrode and the plurality of working electrodes.

[0022] Beneficial effects:

[0023] The present application provides an electrochemical sensor and a method for manufacturing the same, wherein a counter electrode, a reference electrode and a plurality of working electrodes are arranged on a substrate, and the counter electrode and the reference electrode are located in a first region on the substrate, and the working electrode is located in a second region on the substrate, and each working electrode is located in a different sub-region within the second region, and then a paper guide layer is arranged on the substrate, so that the guide layer completely covers the first region and the second region of the substrate; when the electrochemical sensor is used for detection, a drop of a liquid to be detected is allowed to enter the guide layer from the edge of the substrate, and due to the diffusibility of the liquid on the paper material, the liquid to be detected will diffuse on the guide layer, and finally diffuse to the guide layer located in each sub-region within the second region, at which time the reagent enzyme on the working electrode will react with the corresponding component in the liquid to be detected.

[0024] Since each working electrode is located in a different sub-region and the sub-regions are independent of each other, the reactants generated by the reagent enzyme reaction cannot move directly to the position of other working electrodes, thereby reducing the mutual interference between different working electrodes and improving the accuracy of electrochemical sensor detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the description of the embodiments of the present application will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0026] Figure 1 is a schematic structural diagram of an electrochemical sensor proposed in one embodiment of the present application;

[0027] Figure 2 is a schematic side view of the structure of an electrochemical sensor proposed in one embodiment of the present application;

[0028] Figure 3 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0029] Figure 4 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0030] Figure 5 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0031] Figure 6 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0032] Figure 7 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0033] Figure 8 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0034] Fig. 9 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0035] Fig.10 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0036] Fig.11 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0037] Fig.12 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0038] Fig.13 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0039] Fig.14 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0040] Fig.15 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0041] Fig.16 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0042] Fig.17 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0043] Fig.18 is a schematic diagram of the structure of another electrochemical sensor proposed in one embodiment of the present application;

[0044] Fig.19 is a flowchart of a method for manufacturing an electrochemical sensor according to an embodiment of the present application;

[0045] Fig. 20 is a flowchart of a method for manufacturing a guide layer according to an embodiment of the present application;

[0046] Fig.21 1 is a flowchart of another method for manufacturing a guide layer according to an embodiment of the present application.

[0047] Explanation of the reference numerals: 1. substrate; 11. first region; 12. second region; 121. sub-region; 2. power electrode; 21. counter electrode; 22. reference electrode; 3. working electrode; 4. guide layer; 5. insulating layer. DETAILED DESCRIPTION

[0048] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0049] Embodiment 1

[0050] Reference Figure 1As shown, an electrochemical sensor disclosed in an embodiment of the present application includes a substrate 1 and electrodes arranged on the substrate 1. The electrochemical sensor disclosed in the embodiment of the present application is mainly used for detecting various components in liquids.

[0051] Specifically, the substrate 1 can be made of polyester resin or polyimide.

[0052] Reference Figure 1 As shown, the electrode includes a power electrode 2 and a plurality of working electrodes 3. The power electrode 2 serves to connect the circuit and stabilize the voltage. In addition, the surface of the electrode detection end of each working electrode 3 has a reagent enzyme. The reagent enzyme can react with the corresponding analyte in the liquid to be detected, and produce a product through the reagent enzyme reaction. The product will undergo oxidation or reduction reaction on the working electrode 3 on the corresponding sensor to produce a change in the electrical signal. At the same time, the reagent enzyme on each working electrode 3 is different. In this way, a plurality of components in the liquid to be detected can be detected using one electrochemical sensor, which can effectively improve the detection efficiency of the electrochemical sensor. Of course, a biosensor can also be used to detect a component. In this way, the sensors of the plurality of working electrodes 3 will produce changes in the electrical signal, thereby making the detection results after calculation and statistics more accurate. Reference Figure 2 As shown, the leads of the power electrode 2 and the working electrode 3 are covered with an insulating layer 5 .

[0053] Specifically, refer to Figure 1 As shown, the substrate 1 includes a first area 11 and a second area 12, the power electrode is located in the first area 11 of the substrate 1, and the working electrode 3 is located in the second area 12 of the substrate 1. It should be noted that the first area 11 and the second area 12 are not a specific space formed on the substrate 1, but a division of the substrate 1 itself. The sizes of the first area 11 and the second area 12 are set according to actual needs.

[0054] Further, refer to Figure 1 As shown, the second region 12 includes multiple sub-regions 121, and each sub-region 121 is independent of each other, that is, on the substrate 1, the position of each sub-region 121 is separated from each other and has no correlation, and the size of each sub-region 121 can be the same or different; the number of sub-regions 121 corresponds to the number of working electrodes 3, and each working electrode 3 is located in a sub-region 121.

[0055] Reference Figure 1As shown, the electrochemical sensor also includes a guide layer 4, which is a paper material. The paper material itself is a porous material. After the liquid to be detected is dropped on the guide layer 4, the guide layer 4 of the paper material will generate capillary force on the liquid to be detected, so that the liquid to be detected can diffuse on the guide layer 4. The width of the guide layer located in the second area is 10μm-5mm.

[0056] Figure 2 The side structure schematic diagram of the electrochemical sensor is shown in FIG. Figure 2 As shown, the guide layer 4 is located above the power electrode 2 and the working electrode 3, and the guide layer 4 covers the first area 11 and the second area 12 of the substrate 1. Figure 1 As shown, the overall shape of the guide layer 4 is the same as the shape formed by the first area 11 and the second area 12, and the area of ​​the guide layer 4 also corresponds to the area included in the first area 11 and the second area 12. In addition, one edge of the guide layer 4 extends to the edge of the substrate 1, so that the liquid to be detected can enter the guide layer 4 from here.

[0057] It should be noted that Figure 2 It is only a schematic diagram of the electrochemical sensor structure. In practical applications, the thicknesses of the power electrode 2 , the working electrode 3 and the guide layer 4 are all at the micrometer level. Therefore, in fact, the guide layer 4 is closely attached to the substrate 1 .

[0058] When using an electrochemical sensor, the liquid to be detected enters the guide layer 4 from the edge of the substrate 1. Since the liquid will diffuse on the guide layer 4 by itself, the liquid will gradually diffuse from the guide layer 4 located in the first area 11 to the guide layer 4 located in each sub-area 121 of the second area 12. When the liquid to be detected diffuses to the position where the electrode detection end of each working electrode 3 is located, the corresponding components in the liquid to be detected will react with different reagent enzymes on each working electrode 3. The products generated by the reagent enzyme and the corresponding components in the liquid to be detected will cause the working electrode 3 to generate an electrical signal, thereby realizing the detection of various components in the liquid to be detected. Of course, if there is no component in the liquid to be detected that reacts with a certain reagent enzyme, then the corresponding working electrode 3 will not generate an electrical signal.

[0059] Since each sub-region 121 is independent of each other and each sub-region 121 is covered with a guide layer 4, the reactants generated by the reagent enzyme and the corresponding components in the liquid to be detected cannot easily move to the positions of other working electrodes 3 through the guide layer 4. In this way, even if the reactants generated by different reagent enzyme reactions are the same, the mutual interference between each working electrode 3 will be reduced, thereby improving the accuracy of the detection results.

[0060] In specific applications, the guide layer 4 can be fixed to the substrate 1 by gluing, and the guide layer 4 is cut and formed in one piece.

[0061] In one embodiment, the guide layer 4 may be made of filter paper, chromatography paper or blotting paper.

[0062] Filter paper, chromatography paper and blotting paper all have strong water absorption and can better diffuse the liquid on the guide layer 4 .

[0063] The electrode material of the working electrode 3 can be gold, platinum, carbon or a composite material containing gold, platinum or carbon.

[0064] The power supply electrode 2 includes two cases.

[0065] Reference Figure 7 As shown, in the first case, the power electrode 2 only includes the working electrode 21, and the working electrode 21 can simultaneously play the role of connecting the circuit and stabilizing the voltage. At this time, the material of the working electrode 21 is Ag / AgCl, in which the ratio of silver to silver chloride is 1:1, 6:4 or 7:3.

[0066] Reference Figure 3 As shown, in the second case, the power electrode 2 includes a counter electrode 21 and a reference electrode 22, wherein the counter electrode 21 serves to connect the circuit, and the reference electrode 22 serves to stabilize the voltage. At this time, the material of the counter electrode 21 is the same as that of the working electrode 3.

[0067] The electrode material of the reference electrode 22 is Ag / AgCl, wherein the ratio of silver to silver chloride is 1:1, 6:4 or 7:3.

[0068] The plurality of working electrodes are located on one side or both sides of the power electrode; or, the plurality of working electrodes are evenly spaced along the circumference of the power electrode.

[0069] In one embodiment, referring to Figure 3 As shown, the power electrode 2 includes a counter electrode 21 and a reference electrode 22, and there are three working electrodes 3 in total. The electrode detection ends of the three working electrodes 3 are on the same straight line on the substrate 1. Correspondingly, three sub-regions 121 are formed on the substrate 1, each sub-region 121 is in a long strip shape, and the three long strip sub-regions 121 are parallel to each other.

[0070] In one embodiment, referring to Figure 4 As shown, the power electrode includes a counter electrode 21 and a reference electrode 22, and there are three working electrodes 3 in total. The electrode detection ends of the three working electrodes 3 are on the same straight line on the substrate 1. Correspondingly, three sub-regions 121 are formed on the substrate 1, but the shape of each sub-region 121 is different, and the position of each sub-region 121 is also different.

[0071] In one embodiment, referring to Figure 5As shown, the power electrode 2 includes a counter electrode 21 and a reference electrode 22, and there are three working electrodes 3 in total. The electrode detection ends of the three working electrodes 3 are located at different positions on the substrate 1. Correspondingly, three sub-areas 121 are formed on the substrate 1, and the shapes and positions of the three sub-areas 121 are different.

[0072] In one embodiment, referring to Figure 6 As shown, the counter electrode 21 , the reference electrode 22 and the working electrode 3 may also be arranged on the substrate 1 along the vertical direction.

[0073] In one embodiment, referring to Figure 7 As shown, the power electrode 2 only includes an active electrode 21; there are three working electrodes 3 in total, and the electrode detection ends of the three working electrodes 3 are on the same straight line on the substrate 1. Correspondingly, three sub-regions 121 are formed on the substrate 1, each sub-region 121 is in a long strip shape, and the three long strip sub-regions 121 are parallel to each other.

[0074] In one embodiment, referring to Figure 8 As shown, the power electrode 2 only includes an active electrode 21; there are three working electrodes 3 in total, and the electrode detection ends of the three working electrodes 3 are on the same straight line on the substrate 1. Correspondingly, three sub-areas 121 are formed on the substrate 1, but the shape of each sub-area 121 is different, and the position of each sub-area 121 is also different.

[0075] In one embodiment, referring to Fig. 9 As shown, the power electrode 2 only includes an active electrode 21; there are three working electrodes 3 in total, and the electrode detection ends of the three working electrodes 3 are located at different positions on the substrate 1. Correspondingly, three sub-areas 121 are formed on the substrate 1, and the shapes and positions of the three sub-areas 121 are different.

[0076] In one embodiment, referring to Fig.10 As shown, the power electrode 2 only includes an active electrode 21 ; there are three working electrodes 3 in total, and the three working electrodes 3 are arranged on the substrate 1 along the vertical direction.

[0077] In one embodiment, referring to Fig.11 As shown, the power electrode 2 only includes an active electrode 21 ; a total of four working electrodes 3 are provided, and the four working electrodes 3 are respectively located on both sides of the active electrode 21 .

[0078] In one embodiment, referring to Fig.12 As shown, the power electrode 2 only includes the working electrode 21 ; there are four working electrodes 3 in total, and the four working electrodes 3 are evenly distributed along the circumference of the working electrode 21 .

[0079] In one embodiment, referring to Fig.13 As shown, the power electrode 2 only includes the working electrode 21 ; there are six working electrodes 3 in total, and the six working electrodes 3 are evenly distributed along the circumference of the working electrode 21 .

[0080] In one embodiment, referring to Fig.14 As shown, the power electrode 2 only includes the working electrode 21 ; there are twelve working electrodes 3 in total, and the twelve working electrodes 3 are evenly distributed along the circumference of the working electrode 21 in two circles.

[0081] In other embodiments, three, four or more circles of working electrodes 3 may also be provided.

[0082] In one embodiment, referring to Fig.15 As shown, the power electrode 2 includes a counter electrode 21 and a reference electrode 22 ; a total of four working electrodes 3 are provided, and the four working electrodes 3 are respectively located on both sides of the counter electrode 21 and the reference electrode 22 .

[0083] In one embodiment, referring to Fig.16 As shown, the power electrode 2 includes a counter electrode 21 and a reference electrode 22 ; a total of four working electrodes 3 are provided, and the four working electrodes 3 are evenly distributed along the circumference of the counter electrode 21 and the reference electrode 22 .

[0084] In one embodiment, referring to Fig.17 As shown, the power electrode 2 includes a counter electrode 21 and a reference electrode 22 ; a total of six working electrodes 3 are provided, and the six working electrodes 3 are evenly distributed along the circumference of the counter electrode 21 and the reference electrode 22 .

[0085] In one embodiment, referring to Fig.18 As shown, the power electrode 2 includes a counter electrode 21 and a reference electrode 22 ; a total of twelve working electrodes 3 are provided, and the twelve working electrodes 3 are evenly distributed in two circles along the circumference of the counter electrode 21 and the reference electrode 22 .

[0086] It should be noted that, in actual application, the area where the guide layer 4 is located occupies about one fifth of the area of ​​the entire substrate 1 , the guide layer 4 is located above the substrate 1 , and the size of the substrate 1 is generally slender to facilitate the diffusion of liquid on the guide layer 4 .

[0087] Embodiment 2

[0088] Fig.19 A method for manufacturing an electrochemical sensor is shown, referring to Fig.19 As shown, an embodiment of the present application provides a method for manufacturing an electrochemical sensor, the method comprising:

[0089] Step 201: Provide a substrate 1.

[0090] Step 202: forming a power electrode 2 and a plurality of working electrodes 3 on the substrate 1 by a printing process, and drying them.

[0091] Specifically, the printing process can be screen printing. When printing the power electrode 2 and the working electrode 3, the arrangement and position of the power electrode 2 and the working electrode 3 can be set as needed. Hot air drying or moisture absorption drying can be used for drying.

[0092] Step 203: Cover the power electrode 2 and the leads of the plurality of working electrodes 3 with an insulating paste to form an insulating layer 5, and dry the insulating layer.

[0093] Specifically, the insulating paste may be epoxy resin, and the insulating layer 5 plays a role in protecting the electrode.

[0094] Step 205: Print the reagent enzyme on the multiple working electrodes 3 by a printing process and dry them.

[0095] Step 206 : Covering the power electrode 2 and the plurality of working electrodes 3 with a guide layer 4 .

[0096] In one embodiment, referring to Fig. 20 As shown, the step of covering the guide layer 4 on the power electrode 2 and the working electrode includes:

[0097] Step 301 : forming a guide layer 4 on the substrate 1 , and making the guide layer 4 cover the power electrode 2 and the working electrode 3 .

[0098] Specifically, the guide layer 4 can be fixedly connected to the substrate 1 by gluing.

[0099] Step 302: Process the guide layer 4 by etching or shearing process.

[0100] Specifically, the guide layer 4 is etched or sheared so that the shape of the guide layer 4 is the same as the shape formed by the first region 11 and the second region 12. In this way, the guide layer 4 can better guide the liquid to diffuse toward a predetermined position.

[0101] In one embodiment, referring to Fig.21 As shown, the step of covering the guide layer 4 on the power electrode 2 and the working electrode 3 includes:

[0102] Step 401: Processing the guide layer 4 by a shearing process.

[0103] Specifically, the shape of the guide layer 4 is processed to be the same as the shape constituted by the first region 11 and the second region 12 .

[0104] Step 404 : Paste the processed guide layer 4 onto the substrate 1 , so that the guide layer 4 covers the power electrode 2 and the working electrode 3 .

[0105] It should be noted that the various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0106] It should also be noted that, in this article, the orientation or position relationship indicated by the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc. is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application. In addition, relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply that there is any such actual relationship or order between these entities or operations, nor can they be understood as indicating or implying relative importance. Moreover, the terms "include", "comprise" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or terminal device including a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, method, article or terminal device. Without more restrictions, an element defined by the phrase "comprising a ..." does not exclude the existence of other identical elements in the process, method, article or terminal device comprising the element.

[0107] The technical solution provided by the present application is described in detail above. The principle and implementation method of the present application are described in this article using specific examples. The description of the above embodiments is only used to help understand the present application, and the content of this specification should not be understood as limiting the present application. At the same time, for those of ordinary skill in the art, according to the present application, there will be different forms of changes in the specific implementation methods and application scopes. It is not necessary and impossible to list all the implementation methods here, and the obvious changes or modifications derived therefrom are still within the scope of protection of the present application.

Claims

1. An electrochemical sensor, characterized in that: It comprises a substrate (1) and an electrode arranged on the substrate (1); The electrodes include a power supply electrode (2) and a plurality of working electrodes (3), the surfaces of the plurality of working electrodes (3) respectively having a plurality of reagent enzymes, and the reagent enzymes on the surface of each of the working electrodes (3) are different; Wherein, the power electrode (2) is located in a first area (11) of the substrate (1); and the plurality of working electrodes (3) are located in a second area (12) of the substrate (1); The second region (12) comprises a plurality of sub-regions (121), the plurality of sub-regions (121) are independent of each other on the substrate (1), and the plurality of working electrodes (3) are respectively located in different sub-regions (121); It also comprises a guide layer (4), the guide layer (4) is a paper material, the guide layer (4) is located above the electrode, the guide layer (4) covers the first area (11) and the second area (12), and one edge of the guide layer (4) extends to the edge of the substrate (1); The shape of the guide layer (4) is the same as the shape formed by the first region (11) and the second region (12).

2. The electrochemical sensor according to claim 1, characterized in that: The material of the guide layer (4) includes: filter paper, chromatography paper or blotting paper.

3. The electrochemical sensor according to claim 1, characterized in that: The plurality of working electrodes are located on one side or both sides of the power electrode; Alternatively, the plurality of working electrodes are evenly spaced apart along the circumference of the power electrode.

4. The electrochemical sensor according to claim 1, characterized in that: The guide layer located in the second region has a width of 10 μm-5 mm.

5. The electrochemical sensor according to claim 1, characterized in that: The power supply electrode includes: an active electrode.

6. The electrochemical sensor according to claim 1, characterized in that: The power supply electrode includes: a counter electrode and a reference electrode.

7. A method for manufacturing an electrochemical sensor, characterized in that: The method comprises: providing a substrate; A power electrode and a plurality of working electrodes are formed on the substrate by a printing process and dried; the power electrode is located in a first area of ​​the substrate; the plurality of working electrodes are located in a second area of ​​the substrate; the second area includes a plurality of sub-areas, the plurality of sub-areas are independent of each other on the substrate, and the plurality of working electrodes are respectively located in different sub-areas; Covering the power electrode and the leads of the plurality of working electrodes with an insulating paste to form an insulating layer, and drying the layers; Printing reagent enzymes on the plurality of working electrodes by a printing process and drying them; A guide layer is covered on the power electrode and the multiple working electrodes; the guide layer is a paper material, the guide layer is located above the electrodes, the guide layer covers the first area and the second area, and one edge of the guide layer extends to the edge of the substrate; the shape of the guide layer is the same as the shape formed by the first area and the second area.

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