Fixing device, liquid changing device and sequencer

Abstract

The present invention discloses a fixing device, a liquid changing device and a sequencer. The fixing device includes a first fixing component and a second fixing component. The first fixing component includes a first fixing frame, a driving member, a limiting member and a resetting member, and the first fixing frame is provided with a first groove, a first hole and a second hole. The limiting member can move along the second hole to the first groove. The second fixing component includes a second fixing frame, and the second fixing frame is provided with a boss and a fixing structure, and the fixing structure is used to fix the reagent bottle, and the boss is provided with a limited surface. The limiting part provides a limit for the limiting surface of the boss, fixes the boss in the first groove, and when the user drives the driving member, the driving member drives the limiting member to retract from the first groove to the second hole, and the boss loses the displacement limit of the first groove. The operator only needs to grab the first fixing frame with other fingers other than the driving member to disassemble and remove the first fixing frame and the reagent bottle, and the resetting member then drives the limiting member to return from the second hole to the first groove.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and in particular to a fixing device, a liquid changing device and a sequencer. Background Art

[0002] Gene sequencing is a new type of gene detection technology. Targeted diagnosis based on this technology has developed rapidly in recent years. This technology can identify individual diseased genes and carry out targeted prevention and treatment. Gene sequencing usually requires multiple cycles of multiple test reagents and flushing fluids to complete. Sequencer reagent bottles need to be frequently disassembled and cleaned. The existing reagent bottle disassembly method is relatively cumbersome, which brings inconvenience to medical staff and reduces efficiency. Therefore, it is necessary to study and solve this problem. Summary of the invention

[0003] The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, the present invention provides a fixing device, which enables an operator to fix and take a reagent bottle with one hand.

[0004] The present invention also provides a liquid changing device having the above fixing device.

[0005] The present invention also provides a sequencer having the above liquid changing device.

[0006] The fixing device according to the first aspect of the present invention comprises:

[0007] A first fixing assembly, the first fixing assembly comprises a first fixing frame, a limiting assembly and a reset member, the first fixing frame is provided with a first groove, a first hole and a second hole, the first hole is connected to the second hole, an orifice of the second hole is connected to the first groove, the limiting assembly comprises a driving member and a limiting member, the driving member is passed through the first hole, the limiting member is located in the second hole, the limiting member can move along the second hole to the first groove, the driving member is used to drive the limiting member to be retracted from the first groove to the second hole, and the reset member is used to drive the limiting member to return from the second hole to the first groove;

[0008] A second fixing component, the second fixing component includes a second fixing frame, the second fixing frame is provided with a boss and a fixing structure, the boss is inserted into the first groove, the boss can move along the depth direction of the first groove; the fixing structure is used to fix the reagent bottle; the boss is provided with a limiting surface, the limiting surface is used to abut against the limiting member to limit the movement of the boss from the first groove.

[0009] The fixing device according to the embodiment of the present invention has at least the following beneficial effects: the limiting portion provides a limit for the limiting surface of the boss, fixing the boss in the first groove; when the user drives the driving member, the driving member drives the limiting member to be retracted from the first groove to the second hole, and the boss loses the displacement restriction from the first groove; the operator only needs to grab the first fixing frame with fingers other than the driving member to disassemble and remove the first fixing frame and the reagent bottle, and the reset member then drives the limiting member to return from the second hole to the first groove.

[0010] According to some embodiments of the present invention, the driving member includes a button, which is slidably connected to the first fixed frame, and the button can move along the first hole. The button is provided with a first abutment surface, and the angle between the first abutment surface and the sliding direction of the button relative to the first fixed frame is greater than zero. The limiting member is provided with a second abutment surface, and the angle between the second abutment surface and the sliding direction of the limiting member relative to the first fixed frame is greater than zero, and the first abutment surface and the second abutment surface are in contact with each other.

[0011] According to some embodiments of the present invention, the second hole passes through the first fixing frame in a direction away from the boss, and the first fixing assembly further includes a limiting plate, which is detachably connected to the first fixing frame and is used to close or open the second hole.

[0012] According to the second aspect of the present invention, the liquid replacement device includes the fixing device of the first aspect of the present invention, the liquid replacement device also includes a driving component, a first liquid extraction tube and a second liquid extraction tube, the driving component includes a driving motor and an injection pump, the driving motor is used to drive the injection pump to suck or inject liquid, the second fixing frame is also provided with a pipeline, one end of the pipeline passes through the end face of the fixing structure facing the bottle mouth of the reagent bottle, and the other end of the pipeline passes through the end face of the boss facing the bottom of the first groove; the first fixing frame is also provided with a third hole, one end of the third hole passes through the bottom of the first groove, and the other end of the third hole passes through the first fixing frame along the depth direction of the first groove, and the orifice of the third hole close to the bottom of the first groove is arranged opposite to the tube mouth of the pipeline facing the first groove; one end of the first liquid extraction tube is inserted in the pipeline, and the other end of the first liquid extraction tube is used to extract liquid in the reagent bottle; one end of the second liquid extraction tube is inserted in the third hole, and the other end of the second liquid extraction tube is connected to the injection pump.

[0013] The liquid replacement device according to the embodiment of the present invention has at least the following beneficial effects: by applying the fixing device of the first aspect of the present invention, the operator can fix and take the reagent bottle with one hand, thereby improving the liquid replacement efficiency.

[0014] According to some embodiments of the present invention, the fluid exchange device also includes an adapter, which is inserted into the third hole. The adapter is provided with an adapter hole, which passes through two oppositely arranged end surfaces of the adapter, one end of the adapter hole is arranged opposite to the pipe mouth of the pipeline facing the first groove, and one end of the second liquid extraction tube is inserted into the other end of the adapter hole.

[0015] According to some embodiments of the present invention, the fluid exchange device further includes a sealing ring, a sealing groove is provided at one end of the adapter facing the boss, the sealing ring is disposed in the sealing groove, and the sealing ring is used to seal the gap between the adapter and the boss.

[0016] According to some embodiments of the present invention, the fluid exchange device also includes a sealing ring, an annular groove is further provided on the outer surface of the adapter, the sealing ring is sleeved on the annular groove, a second groove is further provided on one end of the boss facing the bottom of the first groove, one end of the adapter is inserted in the second groove, and the other end of the adapter is inserted in the third hole, and the sealing ring is used to seal the gap between the boss and the adapter.

[0017] According to some embodiments of the present invention, the second fixing frame is further provided with at least one ventilation groove, and the ventilation groove is used to connect the inner cavity of the reagent bottle with the outside.

[0018] According to some embodiments of the present invention, the fluid exchange device also includes a fixed seat, the fixed seat is provided with a mounting platform, the mounting platform is provided with a liquid flow groove, the number of the fixing devices is at least one, each of the first fixing frames is detachably connected to the mounting platform, each of the first fixing frames is arranged in parallel along the length direction of the liquid flow groove, and the liquid flow groove is connected to each of the third holes.

[0019] According to a third aspect of the present invention, the sequencer comprises the liquid replacement device of the second aspect of the present invention, the sequencer further comprises a main control board, a control valve, a first conduit, a second conduit, a third conduit, a fourth conduit, a fifth conduit, a processing chip and a waste liquid bottle, the main control board is electrically connected to the drive motor, the main control board is used to control the driving action of the drive motor, the control valve is provided with a drive end, a liquid inlet end, a processing end and a waste liquid end, the control valve is electrically connected to the main control board, the main control board is used to control the opening and closing of each port in the control valve, and the end of the second liquid extraction pipe away from the fixing device Connected to the liquid inlet end, the first conduit and the second conduit are respectively connected to the injection pump and the driving end, the first conduit is used for the injection pump to input liquid, the second conduit is used for the injection pump to output liquid, the third conduit and the fourth conduit are respectively connected to the processing chip and the processing end, the third conduit is used for the processing chip to input liquid, the fourth conduit is used for the processing chip to output liquid, the processing chip is used to sequence the transmitted liquid, the fifth conduit is connected to the waste liquid bottle and the waste liquid end, the waste liquid bottle is used to collect waste liquid after sequencing is completed and the cleaning liquid used to clean the sequencer.

[0020] The sequencer according to the embodiment of the present invention has at least the following beneficial effects: by applying the liquid changing device of the second aspect of the present invention, the operator can fix and take the reagent bottle with one hand, thereby improving the sequencing efficiency.

[0021] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The present invention will be further described below with reference to the accompanying drawings and embodiments, wherein:

[0023] Figure 1 A schematic diagram of a fixing device according to an embodiment of the first aspect of the present invention;

[0024] Figure 2 for Figure 1 A half-section schematic diagram of an embodiment of a fixing device;

[0025] Figure 3 for Figure 1 A half-section schematic diagram of another embodiment of the fixing device;

[0026] Figure 4 for Figure 1 A half-section schematic diagram of the first fixing frame;

[0027] Figure 5 for Figure 2 Schematic diagram of the middle limiter;

[0028] Figure 6 for Figure 1 A bottom view of the second fixing assembly;

[0029] Figure 7 for Figure 6 Schematic diagram of the cross section in the AA direction;

[0030] Figure 8 A schematic diagram of a liquid replacement device according to an embodiment of the second aspect of the present invention;

[0031] Fig. 9 This is a schematic diagram of a sequencer according to an embodiment of the third aspect of the present invention.

[0032] Reference numerals:

[0033] The first fixing frame 100, the first hole 110, the second hole 120, the first groove 130, the third hole 140, the bolt hole 160, the slide groove 170, and the limiting plate 180;

[0034] The driving member 200, the first abutting surface 210, the threaded hole 215, the limiting member 220, the second abutting surface 230, the bolt 250, and the resetting member 260;

[0035] The second fixing frame 300, the boss 310, the second groove 311, the fixing structure 320, the limiting structure 321, the limiting surface 330, the pipeline 340, and the ventilation groove 350;

[0036] A first liquid extraction tube 400, a second liquid extraction tube 410, a sealing ring 420, an adapter 430, an adapter hole 431, a sealing groove 432, and an annular groove 433;

[0037] A fixing seat 500, a mounting platform 510, a liquid flow tank 520, and a reagent bottle 530;

[0038] Main control board 600, drive motor 610, injection pump 620, control valve 630, drive end 631, liquid inlet end 632, processing end 633, waste liquid end 634, processing chip 640, waste liquid bottle 650, fixing device 660, first conduit 671, second conduit 672, third conduit 673, fourth conduit 674, fifth conduit 675. DETAILED DESCRIPTION

[0039] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0040] In the description of the present invention, it should be understood that descriptions involving orientations, such as up, down, front, back, left, right, etc., and orientations or positional relationships indicated are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0041] In the description of the present invention, "several" means more than one, "many" means more than two, "greater than", "less than", "exceed", etc. are understood to exclude the number itself, and "above", "below", "within", etc. are understood to include the number itself. If there is a description of "first" or "second", it is only used for the purpose of distinguishing the technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0042] In the description of the present invention, unless otherwise clearly defined, terms such as setting, installing, connecting, etc. should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in the present invention based on the specific content of the technical solution.

[0043] In the description of the present invention, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0044] The fixing device, the liquid changing device and the sequencer according to the embodiments of the present invention are described below with reference to the accompanying drawings.

[0045] Reference Figures 1 to 6 According to the fixing device 660 of the first embodiment of the present invention, the first fixing assembly includes a first fixing frame 100, a limiting assembly and a reset member 260. The first fixing frame 100 is provided with a first groove 130, a first hole 110 and a second hole 120. The first hole 110 is connected to the second hole 120, and an orifice of the second hole 120 is connected to the first groove 130. The limiting assembly includes a driving member 200 and a limiting member 220. The driving member 200 is penetrated by the first hole 110, and the limiting member 220 is located in the second hole 120. The limiting member 220 can move along the second hole 120 to the first groove 130. The driving member 200 is used to drive the limiting member 220 to retract from the first groove 130 to the second hole 120. The reset member 260 is used to drive the limiting member 220 to return from the second hole 120 to the first groove 130.

[0046] The second fixing component includes a second fixing frame 300, and the second fixing frame 300 is provided with a boss 310 and a fixing structure 320. The boss 310 is inserted into the first groove 130, and the boss 310 can move along the depth direction of the first groove 130; the fixing structure 320 is used to fix the reagent bottle; the boss 310 is provided with a limiting surface 330, and the limiting surface 330 is used to abut against the limiting member 220 to limit the movement of the boss 310 from the first groove 130.

[0047] Reference Figure 2 Specifically, the fixing structure 320 is a fixing groove arranged at one end away from the boss 310, and a limiting structure 321 is arranged in the fixing groove. The limiting structure 321 can be an existing limiting structure 321 such as an internal thread, a clamping boss, etc. The reagent bottle 530 is provided with a corresponding external thread or a corresponding clamping boss or a groove at the bottle mouth, so that the reagent bottle 530 and the second fixing frame 300 can be pre-tightened and fixed. The fixing structure 320 can also be set as a boss structure, and the boss structure is provided with an external thread or a clamping boss 310 or a groove, and the bottle mouth of the reagent bottle 530 is provided with a corresponding internal thread or a corresponding clamping boss 310.

[0048] Reference Figure 2 The boss 310 is inserted into the first groove 130, and the first groove 130 limits the downward displacement and horizontal displacement of the boss 310. The limiting member 220 is inserted into the limiting surface 330 to further limit the displacement of the boss 310 upward from the first groove 130. At this time, the second fixing frame 300 completes the fixation with the first fixing member.

[0049] According to some embodiments of the present invention, when the movement directions of the driving member 200 and the limiting member 220 are consistent, the driving member 200 and the limiting member 220 are fixedly connected or integrally formed. When the second fixing frame 300 needs to be removed, the driving member 200 is pushed along the first hole 110 in the direction away from the boss 310 to drive the limiting member 220 to slide into the second hole 120. At this time, the boss 310 loses the displacement restriction of being moved out of the first groove 130. The operator only needs to grab the first fixing frame 100 with other fingers other than the fingers that push the driving member 200 to disassemble and remove the second fixing frame 300 and the reagent bottle 530.

[0050] According to another embodiment of the present invention, the driving member 200 includes a button, which is slidably connected to the first fixing frame 100 and can move along the first hole 110. The button is provided with a first abutting surface 210, and the angle between the first abutting surface 210 and the sliding direction of the button relative to the first fixing frame 100 is greater than zero. The limiting member 220 is provided with a second abutting surface 230, and the angle between the second abutting surface 230 and the sliding direction of the limiting member 220 relative to the first fixing frame 100 is greater than zero, and the first abutting surface 210 and the second abutting surface 230 are in contact with each other.

[0051] Reference Figure 2 Specifically, when the driving member 200 and the limiting member 220 have different movement directions, the driving force of the driving member 200 needs to be converted into a component force in the same direction as the movement direction of the limiting member 220. Therefore, the first abutting surface 210 is specifically a first inclined surface, and the second abutting surface 230 is specifically a second inclined surface. When the operator presses the driving member 200, the driving member 200 is pressed down, the first abutting surface 210 abuts against the second abutting surface 230, and the limiting member 220 moves to the left due to the left component force of the second abutting surface 230. The limiting member 220 slides into the second hole 120. At this time, the boss 310 loses the upward displacement restriction. The operator only needs to grab the first fixing frame 100 with other fingers other than pressing the driving member 200 to disassemble and remove the second fixing frame 300 and the reagent bottle 530.

[0052] When the operator releases the driving member 200, the limiting member 220 is reset to the initial state to the right due to the right-facing resistance or pulling force of the reset member 260, and the limiting member 220 extends out of the opening of the second hole 120. The driving member 200 is synchronously reset to the initial position by the upward resistance force of the first resistance surface 210. Specifically, the reset member 260 can be a common elastic element such as a spring, a spring tube, a bellows, and an elastic diaphragm, and the elasticity of the elastic element is used to reset the limiting member 220. When the reset member 260 is set on the left side of the limiting member 220, the reset force of the reset member 260 on the limiting member 220 is a resisting force; when the reset member 260 is set on the right side of the limiting member 220, the reset force of the reset member 260 on the limiting member 220 is a pulling force.

[0053] When it is necessary to fix the first fixing frame 100 again, the operator pushes the limiting member 220 to the left toward one end of the limiting member 220 through the boss 310, so that the limiting member 220 is retracted into the second hole 120, and the operator then inserts the boss 310 downward into the first groove 130. When the position of the limiting member 220 is aligned with the position of the limiting surface 330, the limiting member 220 is snapped to the right into the limiting surface 330 due to the supporting force of the reset member 260, thereby completing the fixation of the second fixing frame 300 again.

[0054] Reference Figure 2According to some embodiments of the present invention, when the driving member 200 moves in the up and down directions along the first hole 110, the driving member 200 can be limited by the bolt 250 to prevent the driving member 200 from sliding out of the first hole 110. A bolt hole 160 is also provided at the lower end of the first fixing frame 100. The bolt hole 160 passes through the bottom surface of the second hole 120. A threaded hole 215 is provided at one end of the driving member 200 facing the bolt hole 160. The bolt 250 is penetrated through the bolt hole 160. The upper end of the bolt 250 is threadedly connected with the threaded hole 215. A bolt head is provided at the lower end of the bolt 250. The bolt head is used to abut against the outer surface of the first fixing frame 100 to limit the movement of the bolt 250 upwardly out of the bolt hole 160. Since the driving member 200 and the bolt 250 are threadedly connected, the movement of the driving member 200 out of the first hole 110 is also limited by the bolt head.

[0055] Reference Figure 5 As a further embodiment, the end of the limiting member 220 that abuts against the limiting surface can be a boss structure with a regular shape, or a boss structure with an inclined surface. The inclined surface can reduce the probability of the limiting member 220 and the boss 310 colliding and getting stuck when the first fixing frame 100 and the second fixing frame 300 are assembled. The force in the vertical direction of the boss 310 inserted into the first groove 130 can also be used to push the limiting member 220 into the second hole 120, thereby reducing the difficulty of assembly.

[0056] As a further embodiment, a limiting hole (not shown in the drawings) can be opened at the position where the limiting member 220 and the reset member 260 abut against each other, and the reset member 260, when it is a spring, is inserted into the limiting hole. The direction of the elastic force of the spring on the limiting member 220 is maintained through the limiting hole, which facilitates assembly and reduces the phenomenon of falling off, side sliding, etc. caused by the elastic force of the spring during assembly.

[0057] Reference Figure 2 and Figure 4 According to some embodiments of the present invention, the second hole 120 passes through the first fixing frame 100 in a direction away from the boss 310, and the first fixing assembly also includes a limiting plate 180, which is detachably connected to the first fixing frame 100, and the limiting plate 180 is used to close or open the second hole 120.

[0058] When the limiting member 220, the reset member 260 and the driving member 200 are assembled through the first hole 110, the opening of the first hole 110 needs to be opened larger. Since it is difficult to limit the driving member 200 with a larger opening, the driving member 200 is easy to swing and tilt when pressed or pushed, and the assembly efficiency through the first hole 110 is not high.

[0059] Reference Figure 2 and Figure 4, the second hole 120 penetrates the first fixing frame 100 in the left-right direction. When assembling, the limiting member 220 and the reset member 260 are sequentially inserted from the left opening of the second hole 120, and the driving member 200 is inserted from the first hole 110 so that the first abutting surface 210 and the second abutting surface 230 are aligned. After the assembly is completed, the left opening of the second hole 120 is closed by the limiting plate 180, and the reset member 260 is provided with a limiting abutment. Specifically, the limiting plate 180 can be detachably connected to the first fixing frame 100 by fasteners such as bolts 250, or a slide groove 170 can be provided on the first fixing frame 100, and the limiting plate 180 is embedded in the first fixing frame 100 through the slide groove 170. The limiting plate 180 is fixed in the first fixing frame 100 by the interference fit between the limiting plate 180 and the slide groove 170. When the limiting plate 180 needs to be removed, the limiting plate 180 can be pulled out by force.

[0060] Reference Fig. 9 , the liquid replacement device according to the second embodiment of the present invention comprises the fixing device 660 of the first embodiment of the present invention, the liquid replacement device also comprises a driving assembly, a first liquid extraction tube 400 and a second liquid extraction tube 410, the driving assembly comprises a driving motor 610 and an injection pump 620, the driving motor 610 is used to drive the injection pump 620 to aspirate or inject liquid, the second fixing frame 300 is also provided with a pipe 340, one end of the pipe 340 passes through the fixing structure 320 for facing the end face of the bottle mouth of the reagent bottle 530, and the other end of the pipe 340 passes through the end face of the boss 310 facing the bottom of the first groove 130; the first fixing The frame 100 is also provided with a third hole 140, one end of the third hole 140 passes through the bottom of the first groove 130, and the other end of the third hole 140 passes through the first fixed frame 100 along the depth direction of the first groove 130, and the hole opening of the third hole 140 close to the bottom of the first groove 130 is arranged opposite to the pipe opening of the pipeline 340 facing the first groove 130; one end of the first liquid suction tube 400 is inserted into the pipeline 340, and the other end of the first liquid suction tube 400 is used to suck the liquid in the reagent bottle 530; one end of the second liquid suction tube 410 is inserted into the third hole 140, and the other end of the second liquid suction tube 410 is connected to the injection pump 620.

[0061] Reference Figure 2 It should be noted that the drive motor 610 and the injection pump 620 are the existing drive motor 610 and the injection pump 620, which are not shown in the figure; one end of the first liquid extraction tube 400 extends into the reagent bottle 530 and can touch the bottom of the reagent bottle 530. For the convenience of expression, the partial view of the reagent bottle 530 is hidden in the figure.

[0062] The injection pump 620 is driven by the driving motor 610 to generate negative pressure, and the liquid in the reagent bottle 530 is sucked and transported through the first suction tube 400, the pipeline 340 and the second suction tube 410 by the negative pressure, so as to prevent the liquid from directly contacting the human body. At the same time, by applying the fixing device 660 of the first aspect of the present invention, the operator can fix and take the reagent bottle 530 with one hand, thereby improving the replacement efficiency of the reagent bottle 530 and the liquid replacement efficiency.

[0063] Reference Figure 2 According to some embodiments of the present invention, the liquid replacement device also includes an adapter 430, which is inserted into the third hole 140. The adapter 430 is provided with an adapter hole 431, and the adapter hole 431 passes through two oppositely arranged end surfaces of the adapter 430. One end of the adapter hole 431 is arranged opposite to the pipe mouth of the pipeline 340 facing the first groove 130, and one end of the second liquid extraction tube 410 is inserted into the other end of the adapter hole 431.

[0064] In the actual liquid exchange process, the model of the second liquid extraction tube 410 is often variable, and may have different diameters in different usage scenarios. When the second liquid extraction tube 410 is directly inserted into the third hole 140, the diameter of the third hole 140 also needs to change with the diameter of the second liquid extraction tube 410, so the liquid exchange device will have the problem of low applicability. By providing an adapter 430, when adapting to different models of second liquid extraction tubes 410, it is only necessary to select an adapter 430 whose diameter of the adapter hole 431 matches the diameter of the second liquid extraction tube 410 and insert it into the third hole 140. Since the external dimensions of the adapter 430 remain unchanged, the diameter of the third hole 140 also remains unchanged, thereby improving the practicality of the liquid exchange device.

[0065] Reference Figure 2 According to some embodiments of the present invention, the fluid exchange device further includes a sealing ring 420 , a sealing groove 432 is provided at one end of the adapter 430 facing the boss 310 , the sealing ring 420 is disposed in the sealing groove 432 , and the sealing ring 420 is used to seal the gap between the adapter 430 and the boss 310 .

[0066] Since there is a certain gap between the pipe mouth of the pipe 340 and the adapter hole 431 of the adapter 430 due to processing errors and assembly errors, the liquid may leak in the gap during the liquid replacement process of the liquid replacement device. When the liquid is harmful, it is detrimental to the life safety of the operator, so the gap needs to be sealed.

[0067] Specifically, a sealing groove 432 is provided at the upper end of the adapter 430, and a sealing ring 420 is provided in the sealing groove 432. When the adapter 430 is inserted into the third hole 140, the sealing ring 420 fills the gap between the pipe mouth of the pipeline 340 and the adapter hole 431 of the adapter 430, leaving only the hollow space in the center of the sealing ring 420 for liquid to flow through, thereby reducing the probability of liquid leakage.

[0068] Reference Figure 3 According to some embodiments of the present invention, the fluid exchange device also includes a sealing ring 420, and the outer surface of the adapter 430 is also provided with an annular groove 433, the sealing ring 420 is sleeved in the annular groove 433, and the end of the boss 310 facing the bottom of the first groove 130 is also provided with a second groove 311, one end of the adapter 430 is inserted into the second groove 311, and the other end of the adapter 430 is inserted into the third hole 140, and the sealing ring 420 is used to seal the gap between the boss 310 and the adapter 430.

[0069] The sealing ring 420 is arranged between the pipe mouth of the pipe 340 and the adapter hole 431 of the adapter 430, which has high requirements on the precision of the sealing ring 420 and has higher requirements on the processing error and assembly error of the fixing device 660. Specifically, one end of the adapter 430 is inserted into the second groove 311 opened at the boss 310, and the other end of the adapter 430 is inserted into the third hole 140, so as to make up the gap between the pipe mouth of the pipe 340 and the adapter hole 431 of the adapter 430, and transfer the gap where leakage may occur to the pipe mouth of the pipe 340 at the second groove 311 and the adapter hole 431 where the adapter 430 and the pipe mouth are connected. The sealing ring 420 is mounted on the annular groove 433 opened on the adapter 430. The sealing ring 420 only needs to make up for the gap caused by the circumferential error between the adapter 430 and the second groove 311. The accuracy requirements of the sealing ring 420, the processing error requirements of the fixing device 660 and the assembly error requirements are all reduced, thereby reducing the processing difficulty, reducing the processing cost and increasing the sealing reliability.

[0070] Reference Figure 6 and Figure 7 It should be noted that Figure 6 In order to clearly show the structure of the second fixing frame 300, the reagent bottle 530 is hidden. According to some embodiments of the present invention, the second fixing frame 300 is further provided with at least one ventilation groove 350, and the ventilation groove 350 is used to connect the inner cavity of the reagent bottle 530 with the outside.

[0071] When the driving motor 610 drives the injection pump 620 to suck the liquid in the reagent bottle 530, the pressure in the reagent bottle 530 needs to be balanced to successfully suck out the liquid. Therefore, a venting groove 350 is provided to connect the inner cavity of the reagent bottle 530 with the outside to ensure the pressure balance in the reagent bottle 530. It is understandable that the shape of the venting groove 350 can be as follows: Figure 6 As shown in FIG, the arc shape corresponding to the mouth of the reagent bottle 530 may also be a square, a triangle or other shapes, as long as the inner cavity of the reagent bottle 530 and the outside are connected.

[0072] Reference Figure 8 According to some embodiments of the present invention, the liquid exchange device also includes a fixed seat 500, the fixed seat 500 is provided with a mounting platform 510, the mounting platform 510 is provided with a liquid groove 520, the number of the fixing device 660 is at least one, each first fixing frame 100 is detachably connected to the mounting platform 510, each first fixing frame 100 is arranged in parallel along the length direction of the liquid groove 520, and the liquid groove 520 is connected to each third hole 140.

[0073] Specifically, the position of the liquid passage groove 520 corresponds to the third hole 140, and the second liquid passage pipe passes through the liquid passage groove 520 and is connected to the fixing device 660. The first fixing frame 100 can be detachably connected to the mounting platform 510 by fasteners such as screws or bolts 250; the first fixing frame 100 can also be fixed by glue; the first fixing frame 100 can also be detachably fixed by magnetic attraction by providing magnetic attraction sheets on the mounting platform 510 and the first fixing frame 100.

[0074] Reference Figure 8 When the first fixing frame 100 is detachably connected by fasteners such as screws or bolts 250, corresponding mounting holes are provided on the mounting platform 510, and multiple first fixing frames 100 can be fixed on the fixing seat 500 in sequence at the positions corresponding to the mounting holes. When the liquid exchange device needs to perform large-scale liquid exchange or cross-mixing of multiple liquids, the function can be realized at the same position through the fixing seat 500, thereby realizing large-scale rapid liquid exchange, and reagent bottles 530 of the same batch can be concentrated to prevent different liquids from being mixed.

[0075] Reference Fig. 9According to the third aspect of the present invention, the sequencer includes the liquid replacement device of the second aspect of the present invention, and the sequencer also includes a main control board 600, a control valve 630, a first conduit 671, a second conduit 672, a third conduit 673, a fourth conduit 674, a fifth conduit 675, a processing chip 640 and a waste liquid bottle 650. The main control board 600 is electrically connected to the drive motor 610. The main control board 600 is used to control the driving action of the drive motor 610. The control valve 630 is provided with a driving end 631, a liquid inlet end 632, a processing chip 640, and a waste liquid bottle 650. The control valve 630 is electrically connected to the main control board 600, and the main control board 600 is used to control the flow direction of the liquid in the control valve 630. The end of the second liquid extraction tube 410 away from the fixing device 660 is connected to the liquid inlet end 632. The first conduit 671 and the second conduit 672 are respectively connected to the injection pump 620 and the driving end 631. The first conduit 671 is used for the injection pump 620 to input liquid, and the second conduit 672 is used for the injection pump 620 to output liquid. The third conduit 673 and the fourth conduit 674 are respectively connected to the processing chip 640 and the processing end 633. The third conduit 673 is used for the processing chip 640 to input liquid, and the fourth conduit 674 is used for the processing chip 640 to output liquid. The processing chip 640 is used to sequence the transmitted liquid. The fifth conduit 675 is connected to the waste liquid bottle 650 and the waste liquid end 634. The waste liquid bottle 650 is used to collect waste liquid after sequencing and cleaning liquid for cleaning the sequencer. By applying the liquid changing device of the second embodiment of the present invention, the operator can fix and take the reagent bottle 530 with one hand, thereby improving sequencing efficiency.

[0076] Specifically, when the main control board 600 inputs the sequencing instruction, the driving motor 610 drives the syringe pump 620 to generate negative pressure, and the liquid in the reagent bottle 530 flows to the liquid inlet end 632 through the first pipette, the pipeline 340, and the second pipette. The liquid flows into the syringe pump 620 through the first conduit 671 of the driving end 631 through the liquid inlet end 632, and then the syringe pump 620 generates positive pressure to push the liquid to the driving end 631 through the second conduit 672, and then flows through the third conduit 673 of the processing end 633 into the processing chip 640 to sequence the liquid. After the test is completed, the syringe pump 620 generates negative pressure to suck the liquid back to the control valve 630 through the fourth conduit 674, and generates positive pressure again through the syringe pump 620 to transport the liquid to the waste liquid end 634, and flows into the waste liquid bottle 650 through the fifth conduit 675 for waste treatment. It can be understood that the control valve 630 controls the opening and closing of the inlet and outlet of each port during the liquid transportation process, so there will be no liquid cross flow phenomenon.

[0077] When the inside of the sequencer needs to be cleaned after sequencing is completed, the liquid in the reagent bottle 530 is replaced with cleaning liquid, which can be achieved by inputting a cleaning instruction through the main control board 600. The difference between the cleaning and sequencing instructions is that the processing chip 640 does not operate during the cleaning process.

[0078] It is understood that the number of fixtures 660 may be as follows: Fig. 9 The two shown in the figure may also be one, three or four, as long as a corresponding number of docking ports are provided at the liquid inlet end 632.

[0079] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge of ordinary technicians in the relevant technical field without departing from the purpose of the present invention. In addition, the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

Claims

1. A fixing device, characterized in that: include: A first fixing component, the first fixing component comprises a first fixing frame, a limiting component and a reset component, the first fixing frame is provided with a first groove, a first hole and a second hole, the first hole is connected to the second hole, and an orifice of the second hole is connected to the first groove, the limiting component comprises a driving member and a limiting member, the driving member is penetrated through the first hole, the limiting member is located in the second hole, and the limiting member can move along the second hole to the first groove; the driving member is used to drive the limiting member to be retracted from the first groove to the second hole, the driving member comprises a button, the button is slidably connected to the first fixing frame, the button can move along the first hole, the button is provided with a first abutting surface, the angle between the first abutting surface and the button relative to the sliding direction of the first fixing frame is greater than zero, the limiting member is provided with a second abutting surface, the angle between the second abutting surface and the limiting member relative to the sliding direction of the first fixing frame is greater than zero, and the first abutting surface and the second abutting surface are in contact; the reset component is used to drive the limiting member to return from the second hole to the first groove; A second fixing assembly, the second fixing assembly includes a second fixing frame, the second fixing frame is provided with a boss and a fixing structure, the boss is inserted into the first groove, the boss can move along the depth direction of the first groove; the fixing structure is used to fix the reagent bottle; the boss is provided with a limited position surface, the limited position surface is used to abut against the limiter to limit the movement of the boss from the first groove; The second hole passes through the first fixing frame in a direction away from the boss, and the first fixing assembly further comprises a limit plate, which is detachably connected to the first fixing frame and is used to close or open the second hole and to provide a limit support for the reset member.

2. A liquid replacement device, characterized in that: It includes the fixing device according to claim 1, the liquid exchange device also includes a driving component, a first liquid extraction tube and a second liquid extraction tube, the driving component includes a driving motor and an injection pump, the driving motor is used to drive the injection pump to suck or inject liquid, the second fixing frame is also provided with a pipeline, one end of the pipeline passes through the end face of the fixing structure for facing the bottle mouth of the reagent bottle, and the other end of the pipeline passes through the end face of the boss facing the bottom of the first groove; the first fixing frame is also provided with a third hole, one end of the third hole passes through the bottom of the first groove, and the other end of the third hole passes through the first fixing frame along the depth direction of the first groove, and the orifice of the third hole close to the bottom of the first groove is arranged opposite to the tube mouth of the pipeline facing the first groove; one end of the first liquid extraction tube is inserted in the pipeline, and the other end of the first liquid extraction tube is used to extract liquid in the reagent bottle; one end of the second liquid extraction tube is inserted in the third hole, and the other end of the second liquid extraction tube is connected to the injection pump.

3. The liquid replacement device according to claim 2, characterized in that: It also includes an adapter, which is inserted into the third hole. The adapter is provided with an adapter hole, which passes through two opposite end surfaces of the adapter. One end of the adapter hole is arranged opposite to the pipe mouth of the pipeline facing the first groove, and one end of the second liquid extraction tube is inserted into the other end of the adapter hole.

4. The liquid replacement device according to claim 3, characterized in that: It also includes a sealing ring. A sealing groove is provided at one end of the adapter facing the boss. The sealing ring is arranged in the sealing groove. The sealing ring is used to seal the gap between the adapter and the boss.

5. The liquid replacement device according to claim 3, characterized in that: It also includes a sealing ring, and the outer surface of the adapter is also provided with an annular groove, the sealing ring is sleeved in the annular groove, and the end of the boss facing the bottom of the first groove is also provided with a second groove, one end of the adapter is inserted in the second groove, and the other end of the adapter is inserted in the third hole, and the sealing ring is used to seal the gap between the boss and the adapter.

6. The liquid replacement device according to claim 2, characterized in that: The second fixing frame is also provided with at least one ventilation groove, and the ventilation groove is used to connect the inner cavity of the reagent bottle with the outside.

7. The liquid replacement device according to claim 2, characterized in that: It also includes a fixing seat, the fixing seat is provided with a mounting platform, the mounting platform is provided with a liquid flow groove, the number of the fixing devices is at least one, each of the first fixing frames is detachably connected to the mounting platform, each of the first fixing frames is arranged in parallel along the length direction of the liquid flow groove, and the liquid flow groove is connected to each of the third holes.

8. A sequencer, characterized in that The invention comprises a liquid replacement device according to any one of claims 2 to 7, wherein the sequencer further comprises a main control board, a control valve, a first conduit, a second conduit, a third conduit, a fourth conduit, a fifth conduit, a processing chip and a waste liquid bottle, wherein the main control board is electrically connected to the drive motor, and the main control board is used to control the driving action of the drive motor, the control valve is provided with a drive end, a liquid inlet end, a processing end and a waste liquid end, the control valve is electrically connected to the main control board, and the main control board is used to control the opening and closing of each port in the control valve, the end of the second liquid extraction tube away from the fixing device is connected to the liquid inlet end, the first conduit and the second conduit are respectively connected to the injection pump and the drive end, the first conduit is used for the injection pump to input liquid, the second conduit is used for the injection pump to output liquid, the third conduit and the fourth conduit are respectively connected to the processing chip and the processing end, the third conduit is used for the processing chip to input liquid, the fourth conduit is used for the processing chip to output liquid, and the processing chip is used to sequence the transmitted liquid, the fifth conduit is connected to the waste liquid bottle and the waste liquid end, and the waste liquid bottle is used to collect waste liquid after sequencing and cleaning liquid for cleaning the sequencer.

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