Automatic microorganism sample pretreatment device

Abstract

The utility model discloses an automatic microorganism sample pretreatment device, which relates to the field of pretreatment of legionella pneumophila, and comprises an oscillation module, a stock solution module, a heat treatment module, an acid treatment module, a liquid absorption module, a waste liquid recovery module and a sample inoculation module. Different samples are oscillated and eluted through a test tube oscillator in the oscillation module, the samples in the test tube oscillator are sucked through the liquid suction module, and the same sample is moved into the heat treatment module, the acid treatment module and the stock solution module to be treated; the treated sample is transferred to the sample inoculation module through the liquid suction module for sample inoculation, and waste liquid, waste test tubes and waste liquid suction tubes generated in the period are recycled through the waste liquid recycling module. According to the device, the problems of influence on subsequent sample culture and reduction of the probability of positive results due to confusion operation in busy work are effectively avoided, and the overall high efficiency of the device is improved.

Description

Technical Field

[0001] The present application relates to the field of pre-treatment of Legionella pneumophila, and in particular to an automated microbial sample pre-processor. Background Art

[0002] Traditional pretreatment of Legionella pneumophila generally requires sample precipitation, filtration, and then heat treatment and acid treatment.

[0003] At present, samples are usually extracted manually, loaded into different processing stations, and then the processed samples are inoculated separately.

[0004] However, the manual operation method mentioned above is different in sample volume, sample pH, temperature and time for heat treatment and acid treatment, which can easily cause confusion during busy work, affecting subsequent sample culture and reducing the probability of positive results. Utility Model Content

[0005] In order to avoid confusion during the operation and effectively improve the efficiency of the pre-treatment of Legionella pneumophila, the present application provides an automated microbial sample pre-processor.

[0006] In the first aspect, the present application provides an automated microbial sample preprocessor, which adopts the following technical solution:

[0007] An automated microbial sample preprocessor comprises an oscillation module, wherein the oscillation module comprises a test tube oscillator, and the test tube oscillator is used to oscillate and elute the sample in the test tube;

[0008] A stock solution module, the stock solution module comprising a first mounting seat, wherein a first test tube rack is disposed in the first mounting seat;

[0009] A heat treatment module, wherein the heat treatment module is used to perform water bath heating treatment on the sample;

[0010] An acid treatment module, wherein the acid treatment module is used to adjust the pH value of the sample to a specified value;

[0011] A liquid aspiration module, the liquid aspiration module is used to aspirate the sample eluted in the oscillation module and move it to the stock solution module, the heat treatment module and the acid treatment module respectively, and the liquid aspiration module is used to inoculate the sample in the stock solution module, the heat treatment module and the acid treatment module into the sample inoculation module;

[0012] A waste liquid recovery module, which is used to collect and process waste liquid and waste test tubes;

[0013] The sample inoculation module is used to inoculate the samples in the heat treatment module, the stock solution module and the acid treatment module respectively.

[0014] By adopting the above technical solution, the test tube oscillator in the oscillation module oscillates and elutes different samples, the liquid aspiration module is used to aspirate the samples in the test tube oscillator, and the same sample is moved to the heat treatment module, the acid treatment module and the stock solution module for treatment, and then the liquid aspiration module is moved to the waste liquid recovery module, and the waste liquid and the waste liquid aspiration tube are recovered and processed, the internal samples are processed by the stock solution module, the heat treatment module and the acid treatment module, and the samples processed in the stock solution module, the heat treatment module and the acid treatment module are moved to the sample inoculation module by the liquid aspiration module for sample inoculation, and after the sample inoculation is completed, the liquid aspiration module is moved again to the waste liquid recovery module for waste liquid recovery. The device effectively avoids the problem of confusing operations during busy work, affecting the cultivation of subsequent samples and reducing the probability of positive results, thereby improving the overall efficiency of the device.

[0015] Preferably, the liquid suction module comprises an annular electric slide rail, a base is fixedly arranged on the slider of the annular electric slide rail, a lifting structure is arranged on the base, a first liquid suction plate is arranged on the lifting structure, a rotating structure is arranged between the liquid suction plate and the lifting structure, three rows of first liquid suction tubes are arranged on the first liquid suction plate, and a first fixed structure is arranged between each of the first liquid suction tubes and the first liquid suction plate;

[0016] A second liquid wicking plate is arranged on the side of the lifting structure away from the first liquid wicking plate, and the second liquid wicking plate is connected to the lifting structure via a rotating structure, a stepping structure is arranged between the second liquid wicking plate and the rotating structure, and the stepping structure is used to move the second liquid wicking plate horizontally on the rotating structure, three rows of second liquid wicking tubes are arranged on the second liquid wicking plate, a second fixed structure is arranged between each of the second liquid wicking tubes and the second liquid wicking plate, a telescopic structure is arranged between each of the second liquid wicking tubes and the second liquid wicking plate, and the telescopic structure is used to drive the corresponding second liquid wicking tube to telescope in the second liquid wicking plate;

[0017] The lifting structure is used to synchronously lift the first liquid absorbing plate and the second liquid absorbing plate, and the rotating structure is used to synchronously rotate the first liquid absorbing plate and the second liquid absorbing plate;

[0018] Each of the first and second suction pipes is connected to a suction and discharge air pipe, and one end of each of the suction and discharge air pipes away from the first and second suction pipes is connected to an air source. The top of the first and second suction pipes is fixedly connected to the outer surface of the suction and discharge air pipe, and the suction and discharge air pipe is a telescopic hose.

[0019] By adopting the above technical scheme, the annular electric slide rail drives the base to move, and the first liquid absorption plate and the second liquid absorption plate are moved to the specified position. The height of the first liquid absorption plate and the second liquid absorption plate is adjusted by the lifting structure, and the switching between the first liquid absorption plate and the second liquid absorption plate is achieved by the rotating structure; when the first liquid absorption plate and the second liquid absorption plate are working, the first liquid absorption tube or the second liquid absorption tube is inserted into the test tube to be sampled, and the first liquid absorption tube and the second liquid absorption tube are controlled by the air source to absorb and discharge the air pipe, thereby realizing automatic liquid absorption and discharge, and at the same time, the liquid absorption plate is switched according to the different needs of the liquid absorption module, thereby improving the overall convenience of the device.

[0020] Preferably, the lifting structure comprises a lifting cylinder, the lifting cylinder is vertically arranged on the base, and the push rod of the lifting cylinder is fixedly connected to the rotating structure;

[0021] The rotating structure includes an electric turntable, which is fixed on the push rod of the lifting cylinder. The first liquid absorption plate is connected to one side of the output end of the electric turntable, and the first liquid absorption plate is horizontally arranged. The second liquid absorption plate is connected to the side of the output end of the electric turntable away from the first liquid absorption plate, and the second liquid absorption plate is horizontally arranged.

[0022] By adopting the above technical solution, the lifting cylinder can lift the first and second liquid absorption plates, and the electric turntable can switch between the first and second liquid absorption plates, thereby further improving the convenience of the device as a whole.

[0023] Preferably, each of the first fixing structures comprises a first electromagnet disposed on the first liquid-absorbing plate and a first magnet sleeved on the first liquid-absorbing tube, and the first electromagnet and the first magnet are cooperatively disposed;

[0024] A mounting groove is provided on the second liquid-absorbing plate at a position corresponding to each second liquid-absorbing tube, each of the telescopic structures includes a micro electric push rod arranged in the corresponding mounting groove, each of the second fixed structures includes a magnetic suction seat arranged at the output end of the micro electric push rod, each of the magnetic suction seats is provided with a second electromagnet, each of the second liquid-absorbing tubes is sleeved with a second magnet, and the second electromagnet and the second magnet are arranged in cooperation with each other;

[0025] The stepping structure includes a fixed seat arranged on the rotating structure, a screw is rotatably arranged on the fixed seat, a rotating motor is arranged on one side of the fixed seat on the rotating structure, an output end of the rotating motor is transmission-connected with the screw, one end of the second liquid-absorbing plate is threadedly connected with the screw, and one end of the second liquid-absorbing plate is slidingly limited on the fixed seat.

[0026] By adopting the above technical solution, the electromagnet and the magnet cooperate to realize the fixation and release of the pipette plate to the pipette tube, which is convenient for releasing the waste pipette tube. The second pipette tube is extended or retracted by the micro electric push rod, and the second pipette plate is driven to slide by the rotating motor.

[0027] Preferably, the heat treatment module comprises a water bath, a second test tube rack is arranged on the water bath, and the water bath is used to perform water bath heating on the test tubes on the second test tube rack.

[0028] By adopting the above technical solution, the water bath pot can achieve water bath heating treatment of the sample.

[0029] Preferably, the acid treatment module comprises a second mounting seat, a third test tube rack is arranged on the second mounting seat, and a hydrochloric acid storage tank is arranged on one side of the second mounting seat.

[0030] By adopting the above technical solution, the sample is released into the test tube of the third test tube rack through the liquid aspiration module, and then the hydrochloric acid in the hydrochloric acid storage tank is aspirated and transferred into the test tube where the sample is located, thereby achieving acid treatment of the sample.

[0031] Preferably, the waste liquid recovery module includes a third mounting seat, on which a waste liquid collection box and a waste tube collection box are sequentially arranged, the waste liquid collection box is used to recycle waste liquid, and the waste tube collection box is used to recycle waste test tubes and waste pipettes.

[0032] By adopting the above technical solution, it is convenient to recycle waste liquid, waste test tubes and waste pipettes.

[0033] Preferably, the sample inoculation module includes an inoculation box and an inoculation plate arranged in the inoculation box, a transmission structure is arranged between the inoculation plate and the bottom of the inoculation box, three rows of stepping conveying structures are arranged on the inoculation plate, and each row of the stepping conveying structures is arranged with a plurality of culture dishes, the inoculation box is located above the inoculation plate and a partition is arranged, an inoculation port is opened on the partition, an opening and closing plate is slidably arranged at the inoculation port, an electric push rod is arranged on the partition, and the electric push rod is used to drive the opening and closing plate to open and close the inoculation port.

[0034] By adopting the above technical solution, the transmission structure realizes the pulling and pulling of the inoculation plate relative to the inoculation box. At the same time, the three-row stepping conveying structure is used for samples obtained by three different processing methods, and the inoculation box is closed by the opening and closing plate during non-inoculation time.

[0035] Preferably, three inoculation units are provided on the inoculation plate, each of the inoculation units is provided with a stepping conveying structure, the stepping conveying structure comprises two stepping motors arranged opposite to each other, the two stepping motors are connected by a conveyor belt, an annular slide rail is provided on the outer sides of the two stepping motors, a plurality of inoculation seats are slidably connected to the annular slide rail, a culture dish is fixedly provided on each of the inoculation seats, and a connecting rod is hinged between each of the inoculation seats and the conveyor belt;

[0036] The transmission structure includes a pull-out plate slidably arranged at the bottom of the inoculation box, a pull-out opening is opened on the side of the inoculation box close to the pull-out plate, a telescopic cylinder is horizontally arranged on one side of the inoculation box, and the output end of the telescopic cylinder is fixedly connected to the pull-out plate.

[0037] By adopting the above technical solution, the stepper motor drives the conveyor belt to move, thereby driving the culture dish on the inoculation seat to move along the annular slide rail to realize the progressive movement of the culture dish; at the same time, the telescopic cylinder is used to drive the pull-out plate to slide, so as to realize the insertion and withdrawal of the inoculation plate in the inoculation box, which is convenient for the subsequent processing of the inoculated samples.

[0038] In a second aspect, the present application provides a processing method for an automated microbial sample preprocessor, which adopts the following technical solution:

[0039] A processing method for an automated microbial sample preprocessor comprises the following steps:

[0040] S1: different samples are oscillated and eluted by the test tube oscillator in the oscillation module;

[0041] S2: Use the liquid aspiration module to aspirate the sample in the test tube oscillator, and move the same sample to the heat treatment module, the acid treatment module and the stock solution module for treatment;

[0042] S3: The liquid aspiration module is moved to the waste liquid recovery module, and the waste liquid and the waste liquid aspiration tube are recovered;

[0043] S4: Processing the internal samples through the stock solution module, the heat treatment module and the acid treatment module, and moving the processed samples in the stock solution module, the heat treatment module and the acid treatment module to the sample inoculation module for sample inoculation through the liquid aspiration module;

[0044] S5: After the sample inoculation is completed, the liquid aspiration module moves again to the waste liquid recovery module for waste liquid recovery.

[0045] By adopting the above technical solution, the problem of confusing operations during busy work, affecting the cultivation of subsequent samples and reducing the probability of positive results can be effectively avoided, thereby improving the overall efficiency of the device.

[0046] In summary, the present application includes at least one of the following beneficial technical effects:

[0047] 1. Use the test tube oscillator in the oscillation module to oscillate and elute different samples, use the liquid aspiration module to aspirate the samples in the test tube oscillator, and move the same sample to the heat treatment module, acid treatment module and stock solution module for treatment, then move the liquid aspiration module to the waste liquid recovery module, and recycle the waste liquid and waste liquid aspiration tube, process the internal samples through the stock solution module, heat treatment module and acid treatment module, and move the processed samples in the stock solution module, heat treatment module and acid treatment module to the sample inoculation module through the liquid aspiration module for sample inoculation. After the sample inoculation is completed, the liquid aspiration module is moved again to the waste liquid recovery module for waste liquid recovery. The device effectively avoids confusing operations during busy work, affects the cultivation of subsequent samples, and reduces the probability of positive results, thereby improving the overall efficiency of the device;

[0048] 2. The base is driven to move by the annular electric slide rail to move the first and second liquid-absorbing plates to the designated positions, and the heights of the first and second liquid-absorbing plates are adjusted by the lifting structure, and the first and second liquid-absorbing plates are switched by the rotating structure; when the first and second liquid-absorbing plates are working, the first and second liquid-absorbing tubes are inserted into the sample tube to be sampled, and the first and second liquid-absorbing tubes are sucked and discharged by controlling the air source to draw and discharge the air pipes, thereby realizing automatic liquid suction and discharge, and the liquid-absorbing plates are switched according to the different requirements of the liquid-absorbing modules, thereby improving the overall convenience of the device;

[0049] 3. Comprehensively utilize the lifting cylinder and the electric turntable. The lifting cylinder can lift the first and second liquid absorbing plates. At the same time, the electric turntable can switch between the first and second liquid absorbing plates, further improving the convenience of the device as a whole.

[0050] 4. Comprehensively utilize the cooperation between the electromagnet and the magnet to realize the fixation and release of the pipette by the pipette plate, so as to facilitate the release of the waste pipette;

[0051] 5. The conveyor belt is driven by a stepper motor to move, thereby driving the culture dish on the inoculation seat to move along the annular slide rail to achieve progressive movement of the culture dish; at the same time, the telescopic cylinder is used to drive the pull-out plate to slide, so that the inoculation plate can be inserted and pulled out of the inoculation box, which is convenient for subsequent processing of the inoculated samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 This is an axonometric diagram mainly showing the overall structure of the pre-processor in this embodiment;

[0053] Figure 2This is a top view of the overall structure of the pre-processor mainly embodied in this embodiment;

[0054] Figure 3 This is a schematic diagram mainly showing the structure of the liquid suction module in this embodiment;

[0055] Figure 4 This is a top view of the structure of the liquid suction module mainly embodied in this embodiment;

[0056] Figure 5 This is a cross-sectional view mainly showing the first liquid suction pipe of this embodiment;

[0057] Figure 6 This is a cross-sectional view mainly showing the second liquid suction tube of this embodiment;

[0058] Figure 7 This is a schematic diagram mainly showing the structure of the sample inoculation module in this embodiment;

[0059] Figure 8 This is a transverse cross-sectional view of the transmission structure of this embodiment;

[0060] Fig. 9 This is a vertical cross-sectional view mainly showing the transmission structure of this embodiment;

[0061] Fig.10 It is a partial cross-sectional view of the whole embodiment, mainly showing the structure of the opening and closing plate;

[0062] Fig.11 This is a cross-sectional view mainly showing the test tube installation module structure of this embodiment.

[0063] Figure numerals: 1, oscillation module; 11, test tube oscillator; 2, stock solution module; 21, first mounting seat; 22, first test tube rack; 3, heat treatment module; 31, water bath; 32, second test tube rack; 4, acid treatment module; 41, second mounting seat; 42, third test tube rack; 43, hydrochloric acid storage tank; 5, liquid suction module; 51, annular electric slide rail; 52, base; 53, lifting cylinder; 54, first liquid suction plate; 55, second liquid suction plate; 56, electric turntable; 57, first liquid suction tube; 58, first fixed structure; 581, first electromagnet; 582, first magnet; 59, second liquid suction tube; 510, second fixed structure; 5101, second electromagnet; 5102, second magnet; 511, extraction and discharge air pipe; 512, mounting groove; 513, micro electric push rod; 5 14. Magnetic seat; 515. Stepping structure; 5151. Rotating motor; 5152. Screw rod; 6. Waste liquid recovery module; 61. Third mounting seat; 62. Waste liquid collection box; 63. Waste tube collection box; 7. Sample inoculation module; 71. Inoculation box; 72. Inoculation plate; 73. Transmission structure; 731. Pull-out plate; 732. Telescopic cylinder; 74. Stepping conveying structure; 741. Stepping motor; 742. Transmission belt; 743. Annular slide rail; 744. Inoculation seat; 745. Connecting rod; 75. Culture dish; 76. Partition; 77. Inoculation port; 78. Opening and closing plate; 79. Electric push rod; 8. Test tube installation module; 81. Test tube support frame; 82. Test tube installation plate; 83. Third electromagnet; 84. Third magnet; 9. New pipette installation frame; 10. New test tube installation frame. DETAILED DESCRIPTION

[0064] The present application is further described in detail below in conjunction with the accompanying drawings.

[0065] The embodiment of the present application discloses an automated microbial sample preprocessor.

[0066] Reference Figure 1 to Figure 2 The automated microbial sample preprocessor includes an oscillation module 1, a stock solution module 2, a heat treatment module 3, an acid treatment module 4, a liquid suction module 5, a waste liquid recovery module 6 and a sample inoculation module 7. According to the sample preprocessing requirements, the sample is oscillated and eluted by the oscillation module 1. After the oscillation and elution, the sample is sucked by the liquid suction module 5 and moved to the stock solution module 2, the heat treatment module 3 and the acid treatment module 4 for separate treatment. During this period, the washing liquid module moves the waste liquid in the liquid suction tube and the waste liquid suction tube to the waste liquid recovery module 6 for recycling and treatment, and replaces the new liquid suction tube. After the stock solution module 2, the heat treatment module 3 and the acid treatment module 4 are processed, the liquid suction module 5 moves the processed sample to the sample inoculation module 7 for inoculation to complete the sample preprocessing.

[0067] It should be noted that the oscillation module 1 , stock solution module 2 , heat treatment module 3 , acid treatment module 4 , liquid aspiration module 5 and waste liquid recovery module 6 are located at the top, the sample inoculation module 7 is located at the bottom, and the upper and lower groups are isolated by a partition 76 .

[0068] Reference Figures 1 to 6 The liquid aspiration module 5 is used to aspirate the sample eluted in the oscillation module 1 and move it to the stock solution module 2, the heat treatment module 3 and the acid treatment module 4 respectively, and the liquid aspiration module 5 is used to inoculate the samples in the stock solution module 2, the heat treatment module 3 and the acid treatment module 4 into the sample inoculation module 7.

[0069] The liquid suction module 5 includes an annular electric slide rail 51, a base 52 is fixedly arranged on the slider of the annular electric slide rail 51, a lifting structure is arranged on the base 52, a first liquid suction plate 54 is arranged on the lifting structure, a rotating structure is arranged between the liquid suction plate and the lifting structure, three rows of first liquid suction tubes 57 are arranged on the first liquid suction plate 54, and a first fixed structure 58 is arranged between each first liquid suction tube 57 and the first liquid suction plate 54; a second liquid suction plate 55 is arranged on the side of the lifting structure away from the first liquid suction plate 54, and the second liquid suction plate 55 and the lifting structure are connected. The second liquid absorbing plate 55 is connected to the rotating structure, and a stepping structure 515 is arranged between the second liquid absorbing plate 55 and the rotating structure. The stepping structure 515 is used to move the second liquid absorbing plate 55 horizontally on the rotating structure. Three second liquid absorbing tubes 59 are arranged on the second liquid absorbing plate 55, and a second fixed structure 510 is arranged between each second liquid absorbing tube 59 and the second liquid absorbing plate 55; the lifting structure is used to synchronously lift and lower the first liquid absorbing plate 54 and the second liquid absorbing plate 55, and the rotating structure is used to synchronously rotate the first liquid absorbing plate 54 and the second liquid absorbing plate 55.

[0070] It should be noted that each first suction pipe 57 and second suction pipe 59 is connected to a suction and discharge air pipe 511, and one end of each suction and discharge air pipe 511 away from the first suction pipe 57 and the second suction pipe 59 is connected to an air source (not shown in the figure), and the suction and discharge air pipe 511 is a telescopic hose. The annular electric slide rail 51 drives the base 52 to move, and moves the first liquid absorption plate 54 and the second liquid absorption plate 55 to the specified position. The height of the first liquid absorption plate 54 and the second liquid absorption plate 55 is adjusted by the lifting structure, and the switching between the first liquid absorption plate 54 and the second liquid absorption plate 55 is achieved by the rotating structure; when the first liquid absorption plate 54 and the second liquid absorption plate 55 are working, the first liquid absorption tube 57 or the second liquid absorption tube 59 is inserted into the test tube to be sampled, and the suction and discharge of the first liquid absorption tube 57 and the second liquid absorption tube 59 is achieved by controlling the suction and discharge air pipe 511 through the air source, thereby realizing automatic suction and discharge. At the same time, the suction plate is switched according to the different needs of the suction module 5, thereby improving the overall convenience of the device.

[0071] Each first fixed structure 58 includes a first electromagnet 581 disposed on the first liquid wicking plate 54 and a first magnet 582 sleeved on the first liquid wicking tube 57, and the first electromagnet 581 and the first magnet 582 are arranged in cooperation with each other. Each second fixed structure 510 includes a second electromagnet 5101 disposed on the second liquid wicking plate 55 and a second magnet 5102 sleeved on the second liquid wicking tube 59, and the second electromagnet 5101 and the second magnet 5102 are arranged in cooperation with each other.

[0072] A telescopic structure is provided between each second liquid pipette 59 and the second liquid pipette plate 55, and the telescopic structure is used to drive the corresponding second liquid pipette 59 to telescope in the second liquid pipette plate 55. A mounting groove 512 is provided at a position corresponding to each second liquid pipette 59 on the second liquid pipette plate 55, and each telescopic structure includes a micro electric push rod 513 provided in the corresponding mounting groove 512, and each second fixed structure 510 includes a magnetic seat 514 provided at the output end of the micro electric push rod 513, and each magnetic seat 514 is provided with a second electromagnet 5101, and each second liquid pipette 59 is provided with a second magnet 5102, and the second electromagnet 5101 and the second magnet 5102 are provided in cooperation with each other.

[0073] The stepping structure 515 includes a fixed seat arranged on the rotating structure, a screw rod 5152 is rotatably arranged on the fixed seat, a rotating motor 5151 is arranged on one side of the fixed seat on the rotating structure, an output end of the rotating motor 5151 is transmission-connected to the screw rod 5152, one end of the second liquid wicking plate 55 is threadedly connected to the screw rod 5152, and one end of the second liquid wicking plate 55 is slidably limited on the fixed seat.

[0074] Reference Figure 1 The stock liquid module 2 includes a first mounting seat 21, which is arranged on the inner side of the annular electric slide rail 51. A first test tube rack 22 is arranged in the first mounting seat 21. The annular electric slide rail 51 drives the first liquid absorption plate 54 and the second liquid absorption plate 55 to move to the first mounting seat 21, and absorbs and discharges liquid from the test tubes in the first test tube rack 22.

[0075] Reference Figure 1 The heat treatment module 3 includes a water bath 31, on which a second test tube rack 32 is arranged. The water bath 31 is used to perform water bath heating on the test tubes on the second test tube rack 32. The second test tube rack 32 is mounted on the water bath 31. The annular electric slide rail 51 drives the first liquid absorption plate 54 and the second liquid absorption plate 55 to move to the water bath 31 to absorb and discharge liquid from the test tubes in the second test tube rack 32.

[0076] Reference Figure 1The acid treatment module 4 includes a second mounting seat 41, a third test tube rack 42 is arranged on the second mounting seat 41, and a hydrochloric acid storage tank 43 is arranged on one side of the second mounting seat 41. The annular electric slide rail 51 drives the first liquid absorption plate 54 and the second liquid absorption plate 55 to move to the second mounting plate. When performing acid treatment, the first liquid absorption tube 57 on the first liquid absorption plate 54 first releases the sample into the test tube of the third test tube rack 42, and then the hydrochloric acid in the hydrochloric acid storage tank 43 is moved to the test tube on the third test tube rack 42 through the first liquid absorption tube 57 for acid treatment.

[0077] Reference Figures 7 to 10 The sample inoculation module 7 includes an inoculation box 71 and an inoculation plate 72 disposed in the inoculation box 71. A transmission structure 73 is disposed between the inoculation plate 72 and the bottom of the inoculation box 71. Three rows of step-by-step conveying structures 74 are disposed on the inoculation plate 72. A plurality of culture dishes 75 are disposed on each row of step-by-step conveying structures 74. A partition 76 is disposed above the inoculation plate 72 in the inoculation box 71. An inoculation port 77 is provided on the partition 76. An opening and closing plate 78 is slidably disposed at the inoculation port 77. An electric push rod 79 is disposed on the partition 76. The electric push rod 79 is used to drive the opening and closing plate 78 to open and close the inoculation port 77. The transmission structure 73 realizes the pulling and pulling of the inoculation plate 72 relative to the inoculation box 71. At the same time, the three rows of step-by-step conveying structures 74 are for samples obtained by three different processing methods, and the inoculation box 71 is closed by the opening and closing plate 78 during non-inoculation time.

[0078] It should be noted that three inoculation units are provided on the inoculation plate 72, and each inoculation unit is provided with a stepping conveying structure 74, and the stepping conveying structure 74 includes two relatively arranged stepping motors 741, and the two stepping motors 741 are connected by a transmission belt 742, and an annular slide rail 743 is provided on the outer side of the two stepping motors 741, and a plurality of inoculation seats 744 are slidably connected to the annular slide rail 743, and a culture dish 75 is fixedly provided on each inoculation seat 744, and a connecting rod 745 is hinged between each inoculation seat 744 and the transmission belt 742.

[0079] The transmission structure 73 includes a pull-out plate 731 slidably arranged at the bottom of the inoculation box 71. A pull-out opening is provided on one side of the inoculation box 71 close to the pull-out plate 731. A telescopic cylinder 732 is horizontally arranged at the bottom of the inoculation box 71. The output end of the telescopic cylinder 732 is fixedly connected to the pull-out plate 731.

[0080] The conveyor belt 742 is driven by the stepper motor 741 to move, thereby driving the culture dish 75 on the inoculation seat 744 to move along the annular slide rail 743, thereby realizing the progressive movement of the culture dish 75; at the same time, the telescopic cylinder 732 is used to drive the pull-out plate 731 to slide, thereby realizing the insertion and withdrawal of the inoculation plate 72 in the inoculation box 71, which is convenient for the subsequent processing of the inoculated samples.

[0081] Reference Figure 1 The waste liquid recovery module 6 includes a third mounting seat 61, on which a waste liquid collection box 62 and a waste tube collection box 63 are sequentially arranged. The waste liquid collection box 62 is used to recycle waste liquid, and the waste tube collection box 63 is used to recycle waste test tubes and waste pipettes.

[0082] The waste liquid collection box 62 is used to recycle the waste liquid in the first pipette plate 54 and the second pipette plate 55, and the waste tube collection box 63 is used to recycle the waste pipette tubes in the first pipette plate 54 and the second pipette plate 55 and the waste test tubes of the raw liquid module 2, the heat treatment module 3, and the acid treatment module 4.

[0083] It should be noted that the device also includes a test tube installation module 8, which is used to install test tubes in the stock solution module 2, the heat treatment module 3, and the acid treatment module 4. At the same time, a new pipette installation rack 9 and a new test tube installation rack 10 are provided in conjunction with the test tube installation module 8. The new pipette installation rack 9 is provided with a new pipette for replacement of the pipette module 5, and the new test tube installation rack 10 is provided with a new test tube for replacement of the stock solution module 2, the heat treatment module 3, and the acid treatment module 4.

[0084] Reference Fig.11 The test tube installation module 8 includes a test tube support frame 81 arranged on a slider in the annular electric slide rail 51, a cylinder is arranged between the test tube support frame 81 and the slider, a test tube mounting plate 82 is arranged on the test tube support frame 81, a plurality of test tube installation stations are arranged on the test tube mounting plate 82, and a third electromagnet 83 is arranged in each test tube installation station. The test tubes in the new test tube installation frame 10 and the pipettes of the new pipette installation frame 9 are fixedly sleeved with a third magnet 84 that cooperates with the third electromagnet 83.

[0085] The implementation principle of an automated microbial sample preprocessor and a processing method thereof according to an embodiment of the present application is as follows: the annular electric slide rail 51 drives the test tube rack to sequentially install the test tubes in the new test tube mounting rack 10 into the first test tube rack 22, the second test tube rack 32 and the third test tube rack 42 through the test tube mounting plate 82;

[0086] The sample is preferentially oscillated and eluted by the test tube oscillator 11. After the elution is completed, the annular electric slide rail 51 drives the first liquid-absorbing plate 54 and the second liquid-absorbing plate 55 on the base 52 to move to the test tube oscillator 11, and the electric turntable 56 is controlled to drive the first liquid-absorbing plate 54 toward the test tube oscillator 11, and the lifting cylinder 53 is controlled to drive the first liquid-absorbing plate 54 to descend and the first liquid-absorbing tube 57 is evacuated through the exhaust air pipe 511, so as to absorb the sample in the test tube in the test tube oscillator 11;

[0087] After the absorption is completed, the annular electric slide rail 51 drives the first liquid absorption plate 54 and the second liquid absorption plate 55 on the base 52 to move to the first test tube rack 22, the second test tube rack 32 and the third test tube rack 42 in sequence, and the electric turntable 56 drives the first liquid absorption plate 54 to move toward the first test tube rack 22, the second test tube rack 32 and the third test tube rack 42 in sequence, and blows air to the first liquid absorption tube 57 through the exhaust air pipe 511, so that the same sample is respectively placed in the test tubes in the first test tube rack 22, the second test tube rack 32 and the third test tube rack 42 for processing;

[0088] After the transfer is completed, the annular electric slide rail 51 drives the first liquid pipetting plate 54 to move to the waste liquid collection box 62 and the waste tube collection box 63, and controls the extraction and discharge air pipe 511 to discharge the waste liquid in the first liquid pipetting tube 57 into the waste liquid collection box 62, and then the first electromagnet 581 is powered off, so that the first electromagnet 581 is separated from the first magnet 582, so that the first liquid pipetting tube 57 is placed in the waste tube collection box 63 for collection and processing;

[0089] After the samples in the test tubes in the first test tube rack 22, the second test tube rack 32 and the third test tube rack 42 are processed, the annular electric slide 51 is controlled to drive the second liquid pipetting plate 55 to move to the first test tube rack 22, the second test tube rack 32 and the third test tube rack 42, and the processed samples are absorbed by the second liquid pipette 59, and then the annular electric slide 51 is controlled to drive the second liquid pipetting plate 55 to move to the inoculation box 71, and the electric push rod 79 is controlled to drive the opening and closing plate 78 to open, and the lifting cylinder 53 and the micro The electric push rod 513 moves the second pipette 59 deeper into the inoculation box 71, controls the extraction and discharge air pipe 511 to inoculate the processed sample into the culture dish 75, controls the stepper motor 741 to drive the conveyor belt 742 to move, so that the next culture dish 75 moves to the bottom of the opening and closing plate 78, and repeats the above operation again until all the culture dishes 75 on the inoculation plate 72 are inoculated, controls the telescopic cylinder 732 to drive the pulling plate 731 to move, and realizes the extraction of the inoculation plate 72, so as to facilitate subsequent operations.

[0090] The embodiment of the present application also discloses a processing method of an automated microbial sample preprocessor.

[0091] The processing method of the automated microbial sample preprocessor comprises the following steps:

[0092] S1: different samples are oscillated and eluted by the test tube oscillator 11 in the oscillation module 1;

[0093] S2: Use the liquid aspiration module 5 to aspirate the sample in the test tube oscillator 11, and move the same sample to the heat treatment module 3, the acid treatment module 4 and the stock solution module 2 for treatment;

[0094] S3: the liquid suction module 5 is moved to the waste liquid recovery module 6, and the waste liquid and the waste liquid suction tube are recovered;

[0095] S4: Process the internal samples through the stock solution module 2, the heat treatment module 3 and the acid treatment module 4, and move the processed samples in the stock solution module 2, the heat treatment module 3 and the acid treatment module 4 to the sample inoculation module 7 through the liquid aspiration module 5 for sample inoculation;

[0096] S5: After the sample inoculation is completed, the liquid aspiration module 5 moves again to the waste liquid recovery module 6 to recover the waste liquid.

[0097] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. An automated microbial sample preprocessor, characterized in that: include: An oscillation module (1), the oscillation module (1) comprising a test tube oscillator (11), the test tube oscillator (11) being used to oscillate and elute a sample in a test tube; A stock solution module (2), the stock solution module (2) comprising a first mounting seat (21), a first test tube rack (22) being arranged in the first mounting seat (21); A heat treatment module (3), wherein the heat treatment module (3) is used to perform water bath heating treatment on the sample; An acid treatment module (4), the acid treatment module (4) being used to adjust the pH value of the sample to a specified value; A liquid aspiration module (5), the liquid aspiration module (5) being used to aspirate the sample eluted in the oscillation module (1) and move it to the stock solution module (2), the heat treatment module (3) and the acid treatment module (4), respectively, and the liquid aspiration module (5) being used to inoculate the sample in the stock solution module (2), the heat treatment module (3) and the acid treatment module (4) into the sample inoculation module (7); A waste liquid recovery module (6), the waste liquid recovery module (6) is used to collect and process waste liquid and waste test tubes; A sample inoculation module (7), wherein the sample inoculation module (7) is used to respectively inoculate samples in the heat treatment module (3), the stock solution module (2) and the acid treatment module (4).

2. The automated microbial sample preprocessor according to claim 1, characterized in that: The liquid absorption module (5) comprises an annular electric slide rail (51), a base (52) is fixedly arranged on a slider of the annular electric slide rail (51), a lifting structure is arranged on the base (52), a first liquid absorption plate (54) is arranged on the lifting structure, a rotating structure is arranged between the liquid absorption plate and the lifting structure, three rows of first liquid absorption tubes (57) are arranged on the first liquid absorption plate (54), and a first fixed structure (58) is arranged between each of the first liquid absorption tubes (57) and the first liquid absorption plate (54); A second liquid absorbing plate (55) is arranged on the side of the lifting structure facing away from the first liquid absorbing plate (54), and the second liquid absorbing plate (55) is connected to the lifting structure via a rotating structure, a stepping structure (515) is arranged between the second liquid absorbing plate (55) and the rotating structure, and the stepping structure (515) is used to move the second liquid absorbing plate (55) horizontally on the rotating structure, three rows of second liquid absorbing tubes (59) are arranged on the second liquid absorbing plate (55), a second fixed structure (510) is arranged between each of the second liquid absorbing tubes (59) and the second liquid absorbing plate (55), and a telescopic structure is arranged between each of the second liquid absorbing tubes (59) and the second liquid absorbing plate (55), and the telescopic structure is used to drive the corresponding second liquid absorbing tube (59) to telescope in the second liquid absorbing plate (55); The lifting structure is used to synchronously lift the first liquid absorbing plate (54) and the second liquid absorbing plate (55), and the rotating structure is used to synchronously rotate the first liquid absorbing plate (54) and the second liquid absorbing plate (55); Each of the first liquid suction pipe (57) and the second liquid suction pipe (59) is connected to a suction and discharge air pipe (511); one end of each of the suction and discharge air pipes (511) away from the first liquid suction pipe (57) and the second liquid suction pipe (59) is connected to an air source; the top of the first liquid suction pipe (57) and the second liquid suction pipe (59) is fixedly connected to the outer surface of the suction and discharge air pipe (511); and the suction and discharge air pipe (511) is a telescopic hose.

3. The automated microbial sample preprocessor according to claim 2, characterized in that: The lifting structure comprises a lifting cylinder (53), wherein the lifting cylinder (53) is vertically arranged on a base (52), and a push rod of the lifting cylinder (53) is fixedly connected to the rotating structure; The rotating structure comprises an electric turntable (56), wherein the electric turntable (56) is fixedly arranged on a push rod of a lifting cylinder (53), the first liquid absorption plate (54) is connected to one side of an output end of the electric turntable (56), and the first liquid absorption plate (54) is arranged horizontally, and the second liquid absorption plate (55) is connected to a side of the output end of the electric turntable (56) away from the first liquid absorption plate (54), and the second liquid absorption plate (55) is arranged horizontally.

4. The automated microbial sample preprocessor according to claim 3, characterized in that: Each of the first fixed structures (58) comprises a first electromagnet (581) arranged on the first liquid absorption plate (54) and a first magnet (582) sleeved on the first liquid absorption tube (57), and the first electromagnet (581) and the first magnet (582) are arranged in cooperation with each other; A mounting groove (512) is provided on the second liquid-absorbing plate (55) at a position corresponding to each second liquid-absorbing tube (59); each of the telescopic structures comprises a micro-electric push rod (513) arranged in the corresponding mounting groove (512); each of the second fixed structures (510) comprises a magnetic seat (514) arranged at the output end of the micro-electric push rod (513); each of the magnetic seats (514) is provided with a second electromagnet (5101); each of the second liquid-absorbing tubes (59) is sleeved with a second magnet (5102); and the second electromagnet (5101) and the second magnet (5102) are arranged in coordination with each other; The stepping structure (515) comprises a fixed seat arranged on the rotating structure, a screw rod (5152) is rotatably arranged on the fixed seat, a rotating motor (5151) is arranged on the rotating structure at one side of the fixed seat, an output end of the rotating motor (5151) is transmission-connected to the screw rod (5152), one end of the second liquid absorbing plate (55) is threadedly connected to the screw rod (5152), and one end of the second liquid absorbing plate (55) is slidably limited on the fixed seat.

5. The automated microbial sample preprocessor according to claim 1, characterized in that: The heat treatment module (3) comprises a water bath (31), a second test tube rack (32) being arranged on the water bath (31), and the water bath (31) is used to perform water bath heating on the test tubes on the second test tube rack (32).

6. The automated microbial sample preprocessor according to claim 1, characterized in that: The acid treatment module (4) comprises a second mounting seat (41), a third test tube rack (42) is arranged on the second mounting seat (41), and a hydrochloric acid storage tank (43) is arranged on one side of the second mounting seat (41).

7. The automated microbial sample preprocessor according to claim 1, characterized in that: The waste liquid recovery module (6) comprises a third mounting seat (61), on which a waste liquid collection box (62) and a waste tube collection box (63) are arranged in sequence, the waste liquid collection box (62) being used for recovering waste liquid, and the waste tube collection box (63) being used for recovering waste test tubes and waste pipettes.

8. The automated microbial sample preprocessor according to claim 1, characterized in that: The sample inoculation module (7) comprises an inoculation box (71) and an inoculation plate (72) arranged in the inoculation box (71); a transmission structure (73) is arranged between the inoculation plate (72) and the bottom of the inoculation box (71); three rows of stepping conveying structures (74) are arranged on the inoculation plate (72); a plurality of culture dishes (75) are arranged on each row of the stepping conveying structures (74); a partition (76) is arranged above the inoculation box (71) and an inoculation port (77) is provided on the partition (76); an opening and closing plate (78) is slidably arranged at the inoculation port (77); an electric push rod (79) is arranged on the partition (76); the electric push rod (79) is used to drive the opening and closing plate (78) to open and close the inoculation port (77).

9. The automated microbial sample preprocessor according to claim 8, characterized in that: The inoculation plate (72) is provided with three inoculation units, each of which is provided with a stepping conveying structure (74), the stepping conveying structure (74) comprising two stepping motors (741) arranged opposite to each other, the two stepping motors (741) being connected via a conveying belt (742), an annular slide rail (743) being provided on the outer sides of the two stepping motors (741), a plurality of inoculation seats (744) being slidably connected to the annular slide rail (743), each of which is fixedly provided with a culture dish (75), and a connecting rod (745) being hinged between each of the inoculation seats (744) and the conveying belt (742); The transmission structure (73) includes a pull-out plate (731) slidably arranged at the bottom of the inoculation box (71), a pull-out opening is provided on a side of the inoculation box (71) close to the pull-out plate (731), a telescopic cylinder (732) is horizontally arranged on one side of the inoculation box (71), and an output end of the telescopic cylinder (732) is fixedly connected to the pull-out plate (731).

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