Sample analysis apparatus, reagent adding device, and control method thereof

Abstract

The application provides a reagent adding device, a first reagent needle can perform cleaning, reagent suction, reagent heating and reagent discharge operations; a second reagent needle can perform cleaning, reagent suction, reagent heating and reagent discharge operations; when one of the first reagent needle and the second reagent needle performs the heating operation, the other one can perform one or more combined operations of cleaning, reagent suction, reagent heating and reagent discharge, so that the utilization rate of a driving mechanism is improved and the efficiency of a sample analysis device is improved. The application also provides a sample analysis device and a control method of the reagent adding device.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of medical equipment, in particular to a sample analysis device, a reagent adding device and a control method thereof. BACKGROUND

[0002] The automatic coagulation analyzer is an instrument for analyzing the coagulation and anticoagulation, fibrinolysis and antifibrinolysis functions of blood. The automatic coagulation analyzer generally includes a sample storage device, a reagent refrigeration device, a testing device, a sample adding device, a cup grabbing device and an automatic cup feeding device. The working process is basically as follows: the automatic cup feeding device transports the test cup to the cup grabbing position, the cup grabbing hand in the cup grabbing device transports the blood coagulation test cup from the cup grabbing position to the test position of the testing device, the sample adding needle in the sample adding device transports the blood sample in the sample storage device and the reaction reagent in the reagent refrigeration device into the test cup in the test position, the sample and the reagent react in the test cup, and the testing device measures the reaction result.

[0003] At present, in the field of automatic detection of medical instruments, a reagent needle is generally provided to transfer the reagent from the reagent refrigeration device to the test position of the testing device. Usually, the reagent needle and its driving assembly are horizontally driven in the X / Y direction, and the reagent needle has a rotary rocker structure to drive its rotation to realize the transfer of the reagent. A reagent needle is arranged on the rotary rocker structure. When performing the heating operation, the reagent needle needs to wait for several seconds to heat the reagent in the reagent needle, and after the heating is completed, other operations such as adding reagent can be performed. That is, the reagent needle cannot perform other operations when performing the heating operation, and the driving mechanism will be in a stationary state to wait for the reagent needle to be heated before driving the reagent needle to rotate. This will cause resource waste, low utilization rate of the driving assembly, and thus affect the operation efficiency of the automatic coagulation analyzer, and cannot make the automatic coagulation analyzer achieve better functions. SUMMARY

[0004] Therefore, it is necessary to provide a reagent adding device capable of improving the utilization rate and thus improving the operation efficiency in view of the problem of low utilization rate of the driving assembly caused by the transfer of the reagent by one reagent needle, and to provide a control method applied to the reagent adding device and a sample analysis device containing the reagent adding device.

[0005] The above-mentioned purpose is achieved by the following technical solutions:

[0006] A reagent adding device, comprising:

[0007] A driving mechanism for driving the movement of the reagent needle mechanism;

[0008] A fixed frame connected to the driving mechanism, the fixed frame comprising a first fixed end and a second fixed end;

[0009] The reagent needle mechanism comprises a first reagent needle and a second reagent needle, the first reagent needle is arranged on the first fixed end, and the second reagent needle is arranged on the second fixed end;

[0010] The heating mechanism is used for heating at least one of the first reagent needle and the second reagent needle;

[0011] The cleaning mechanism is used for cleaning the first reagent needle and the second reagent needle;

[0012] The first reagent needle can perform cleaning, reagent suction, reagent heating and reagent discharge operations; the second reagent needle can perform cleaning, reagent suction, reagent heating and reagent discharge operations; when one of the first reagent needle and the second reagent needle performs the heating operation, the other one can perform one or more combined operations of cleaning, reagent suction, reagent heating and reagent discharge.

[0013] In one of the embodiments, the included angle between the line connecting the first fixed end and the center of the fixed frame and the line connecting the second fixed end and the center of the fixed frame ranges from 30° to 180°.

[0014] In one of the embodiments, the included angle between the line connecting the first fixed end and the center of the fixed frame and the line connecting the second fixed end and the center of the fixed frame is 180°.

[0015] In one of the embodiments, the heating mechanism comprises a first heating component and a second heating component, the first heating component is used for heating the reagent in the first reagent needle, and the second heating component is used for heating the reagent in the second reagent needle.

[0016] In one of the embodiments, the reagent adding device further comprises a control mechanism, the control mechanism comprises a first temperature control mainboard and a second temperature control mainboard, the first temperature control mainboard and the second temperature control mainboard are arranged on the fixed frame respectively;

[0017] The first temperature control mainboard is electrically connected with the first heating component, and is used for controlling the heating temperature of the reagent by the first heating component; the second temperature control mainboard is electrically connected with the second heating component, and is used for controlling the heating temperature of the reagent by the second heating component.

[0018] In one of the embodiments, the control mechanism further comprises a first detection mainboard and a second detection mainboard, the first detection mainboard and the second detection mainboard are arranged on the fixed frame;

[0019] The first detection mainboard is used for detecting the liquid level height in the reagent bottle for reagent suction by the first reagent needle; and the second detection mainboard is used for detecting the liquid level height in the reagent bottle for reagent suction by the second reagent needle.

[0020] In one of the embodiments, the cleaning mechanism comprises a cleaning main pipe, a first cleaning branch pipe and a second cleaning branch pipe;

[0021] One end of the first cleaning branch pipe is in communication with the first reagent needle, and the other end of the first cleaning branch pipe is in communication with the cleaning main pipe; one end of the second cleaning branch pipe is in communication with the second reagent needle, and the other end of the second cleaning branch pipe is in communication with the cleaning main pipe.

[0022] In one of the embodiments, a cleaning main valve is arranged on the cleaning main pipe, a first cleaning branch valve is arranged on the first cleaning branch pipe, and a second cleaning branch valve is arranged on the second cleaning branch pipe; wherein,

[0023] The cleaning main valve and the first cleaning branch valve are opened, and the second cleaning branch valve is closed to control the cleaning of the first reagent needle;

[0024] The first cleaning branch valve is opened, and the cleaning main valve and the second cleaning branch valve are closed to control the reagent suction or reagent discharge of the first reagent needle.

[0025] In one of the embodiments, the cleaning main valve and the first cleaning branch valve are both closed to control the heating of the reagent in the first reagent needle.

[0026] In one of the embodiments, the driving mechanism comprises a rotating shaft, a first driving assembly and a second driving assembly, the fixing frame is arranged on the rotating shaft, the first driving assembly is installed on the second driving assembly, and the first driving assembly and the second driving assembly are connected with the rotating shaft, the first driving assembly is used to drive the rotating shaft to rotate, and the second driving assembly is used to drive the rotating shaft to move up and down.

[0027] Also related to a control method of a reagent adding device, comprising the following steps:

[0028] Driving the first reagent needle and the second reagent needle to move, and the first reagent needle and the second reagent needle perform cleaning, reagent suction, reagent heating and reagent discharge operations;

[0029] When the reagent in the first reagent needle is heated, the second reagent needle rotates to the corresponding position to perform one or more combined operations of cleaning, reagent suction, reagent heating and reagent discharge.

[0030] In one of the embodiments, when the second reagent needle performs multiple combined operations, the second reagent needle is circularly executed according to the arrangement order of cleaning, reagent suction, reagent heating and reagent discharge.

[0031] In one of the embodiments, when the reagent in the second reagent needle is heated, the first reagent needle rotates to a corresponding position to sequentially perform one or more combined operations of ejecting reagent, cleaning, sucking reagent and heating reagent.

[0032] In one of the embodiments, when the first reagent needle performs the cleaning operation, the cleaning liquid path of the first reagent needle is connected, and after the cleaning is completed, the cleaning liquid path is disconnected.

[0033] When the first reagent needle performs the reagent sucking operation, the suction and ejection liquid path of the first reagent needle is connected, and after the reagent sucking is completed, the suction and ejection liquid path is disconnected.

[0034] When the first reagent needle performs the reagent ejecting operation, the suction and ejection liquid path of the first reagent needle is connected, and after the reagent ejecting is completed, the suction and ejection liquid path is disconnected.

[0035] The application also relates to a sample analysis device comprising a sample incubation device, a reagent storage device, a sample detection device and the reagent adding device as described in any of the technical features above.

[0036] The sample incubation device and the reagent storage device are arranged side by side, the sample detection device is arranged on one side of the sample incubation device and the reagent storage device, and the reagent adding device is arranged among the sample incubation device, the reagent storage device and the sample detection device, and the reagent adding device can transfer the reagent in the reagent storage device to the sample incubation device or the sample detection device.

[0037] In one of the embodiments, when the first reagent needle of the reagent adding device sucks the reagent in the reagent storage device, the space where the second reagent needle is located is a first avoiding space.

[0038] When the first reagent needle of the reagent adding device ejects the reagent on the sample incubation device, the space where the second reagent needle is located is a second avoiding space.

[0039] When the first reagent needle of the reagent adding device ejects the reagent on the sample detection device, the space where the second reagent needle is located is a third avoiding space.

[0040] When the first reagent needle of the reagent adding device performs the cleaning operation, the space where the second reagent needle is located is a fourth avoiding space.

[0041] In one of the embodiments, the sample analysis device further comprises a waterproof component, the sample detection device comprises a magnetic bead detection mechanism, and the waterproof component is arranged between the magnetic bead detection mechanism and the reagent storage device.

[0042] The application has the following beneficial effects:

[0043] The sample analysis device, the reagent adding device and the control method thereof of the present application, the first reagent needle and the second reagent needle are arranged on the first fixed end and the second fixed end of the fixed frame respectively, so that the driving mechanism can drive the first reagent needle and the second reagent needle to move up and down, so that the first reagent needle and the second reagent needle are rotated to the position of sucking or discharging reagent; the driving mechanism drives the first reagent needle and the second reagent needle to move up and down, so that the first reagent needle and the second reagent needle can suck or discharge reagent; and when the first reagent needle sucks or discharges reagent, the second reagent needle can heat the reagent stored therein, so that the second reagent needle after heating can transfer the reagent, and the first reagent needle not yet heated can be heated at the same time. The problem of low utilization rate of the driving assembly caused by the transfer of reagent by one reagent needle is effectively solved, the second reagent needle heats the reagent therein while the first reagent needle sucks or discharges reagent, the utilization rate of the driving mechanism is improved, the efficiency of reagent transfer is improved, the efficiency of the sample analysis device is improved, and the sample analysis device can play a better effect. BRIEF DESCRIPTION OF DRAWINGS

[0044] Figure 1 It is a perspective view of the reagent adding device of an embodiment of the present application;

[0045] Figure 2 It is a perspective view of the reagent adding device of an embodiment of the present application; Figure 1 It is a top view of the reagent adding device in the sample analysis device;

[0046] Figure 3 It is a liquid path diagram of the first reagent needle and the second reagent needle in the reagent adding device of an embodiment of the present application;

[0047] Figure 4 It is a work flow diagram of the reagent adding device 100 of an embodiment of the present application;

[0048] Figure 5 It is a work flow diagram of the first reagent needle and the second reagent needle; Figure 4 It is a work flow diagram of the first reagent needle and the second reagent needle;

[0049] Figure 6 It is a work flow diagram of the first reagent needle;

[0050] Among them:

[0051] 100-reagent adding device;

[0052] 110-driving mechanism;

[0053] 111-rotating shaft;

[0054] 112-first driving assembly; 1121-first driving motor; 1122-first transmission part;

[0055] 113 - second driving assembly; 1131 - second driving motor; 1132 - second transmission component;

[0056] 120 - fixing frame;

[0057] 121 - first rocker arm;

[0058] 122 - second rocker arm;

[0059] 130 - reagent needle mechanism;

[0060] 131 - first reagent needle;

[0061] 132 - second reagent needle;

[0062] 140 - support frame;

[0063] 150 - control mechanism;

[0064] 151 - first temperature control mainboard;

[0065] 152 - second temperature control mainboard;

[0066] 153 - first detection mainboard;

[0067] 154 - second detection mainboard;

[0068] 160 - cleaning mechanism;

[0069] 161 - cleaning main pipe;

[0070] 162 - first cleaning branch pipe;

[0071] 163 - second cleaning branch pipe;

[0072] 164 - cleaning main valve;

[0073] 165 - first cleaning branch valve;

[0074] 166 - second cleaning branch valve;

[0075] 167 - pump;

[0076] 200 - reagent storage device;

[0077] 300 - sample incubation device;

[0078] 400 - sample detection device. DETAILED DESCRIPTION

[0079] In order to make the objects, technical solutions and advantages of the present application clearer, the sample analysis device, reagent adding device and control method thereof of the present application are further described in detail below with reference to the embodiments and the accompanying drawings. It should be understood that the specific embodiments described herein are only used to explain the present application and not to limit the present application.

[0080] The serial numbers of components in the present application, such as "first", "second", etc., are only used to distinguish the described objects and do not have any sequence or technical meaning. The "connection" and "coupling" in the present application include direct and indirect connection (coupling) unless otherwise specified. In the description of the present application, it should be understood that the orientations or positional relationships indicated by the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0081] In the present application, unless otherwise explicitly specified and limited, the "on" or "under" of the first feature to the second feature can be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the "over", "above" and "on" of the first feature to the second feature can be that the first feature is directly above or obliquely above the second feature, or only means that the horizontal height of the first feature is higher than that of the second feature. The "under", "below" and "under" of the first feature to the second feature can be that the first feature is directly below or obliquely below the second feature, or only means that the horizontal height of the first feature is less than that of the second feature.

[0082] Referring to Figure 1 and Figure 2 , Figure 1 a perspective view of a reagent adding device 100 according to an embodiment of the present application, Figure 2 a top view of the reagent adding device 100 shown in Figure 1 The reagent adding device 100 according to the present application is applied in a sample analysis device, and is used to transfer reagents from a reagent storage device 200 to a sample incubation device 300 or a sample detection device 400 of the sample analysis device, so as to mix the reagents with samples and then detect the samples. After the sample analysis device adopts the reagent adding device 100 according to the present application, the utilization rate of the driving mechanism 110 can be improved, and then the efficiency of reagent transfer and the efficiency of the sample analysis device can be improved, so that the sample analysis device can play a better effect.

[0083] In the present application, the reagent adding device 100 comprises a driving mechanism 110, a fixing frame 120, a reagent needle mechanism 130, a heating mechanism (not shown) and a support frame 140. The support frame 140 can support and fix the driving mechanism 110, and the driving mechanism 110 is arranged on the support frame 140 and connected and fixed by the support frame 140, so that the driving mechanism 110 can be reliably installed and fixed, ensuring that the driving mechanism 110 moves accurately and reliably. The driving mechanism 110 is used to output rotary motion and reciprocating lifting motion, and the driving mechanism 110 is connected with the fixing frame 120, and the reagent needle mechanism 130 is installed on the fixing frame 120. In this way, the driving mechanism 110 can drive the fixing frame 120 to move up and down and rotate, and then the fixing frame 120 drives the reagent needle mechanism 130 to move up and down and rotate. The reagent needle mechanism 130 is used to transfer reagents, and the reagents are transferred to the sample incubation device 300 or the sample detection device 400. The reagent needle mechanism 130 can perform the operation of sucking or spitting reagents when the fixing frame 120 drives the reagent needle mechanism 130 to move up and down; when the fixing frame 120 drives the reagent needle mechanism 130 to rotate, the reagent needle mechanism 130 can be rotated to the corresponding position, such as the reagent sucking station of the reagent storage device 200, the first reagent adding station of the sample incubation device 300 or the second reagent adding station of the sample detection device 400, so as to facilitate the reagent needle mechanism 130 to perform the operation of sucking or spitting reagents.

[0084] Specifically, the fixed frame 120 comprises a first fixed end and a second fixed end. The reagent needle mechanism 130 comprises a first reagent needle 131 and a second reagent needle 132, the first reagent needle 131 is arranged on the first fixed end, and the second reagent needle 132 is arranged on the second fixed end. Optionally, the fixed frame 120 comprises a first rocker arm 121 and a second rocker arm 122, one end of the first rocker arm 121 is fixed on the driving mechanism 110, the other end of the first rocker arm 121 extends away from the driving mechanism 110, one end of the second rocker arm 122 is also fixed on the driving mechanism 110, and the other end of the second rocker arm 122 also extends away from the driving mechanism 110. The first fixed end is located on the end of the first rocker arm 121 away from the driving mechanism 110, and the second fixed end is located on the end of the second rocker arm 122 away from the driving mechanism 110. The first reagent needle 131 is fixed on the first fixed end of the first rocker arm 121, and the second reagent needle 132 is fixed on the second fixed end of the second rocker arm 122. Moreover, the end of the first rocker arm 121 connected with the driving mechanism 110 coincides with the end of the second rocker arm 122 connected with the driving mechanism 110, that is, the first rocker arm 121 and the second rocker arm 122 are arranged in the same plane, so that the first reagent needle 131 and the second reagent needle 132 are at the same height, facilitating the operation of sucking and discharging reagents. The driving mechanism 110 can drive the first rocker arm 121 and the second rocker arm 122 to rotate and move up and down, and then the first rocker arm 121 drives the first reagent needle 131 to move, and the second rocker arm 122 drives the reagent needle to move, so that the first reagent needle 131 and the second reagent needle 132 can move synchronously.

[0085] Specifically, the heating mechanism is arranged in the first reagent needle 131 and / or the second reagent needle 132, that is, at least one of the first reagent needle 131 and the second reagent needle 132 is provided with the heating mechanism. The heating mechanism can heat the reagent in at least one of the first reagent needle 131 and the second reagent needle 132. The heating mechanism can heat the reagent in the first reagent needle 131 alone, heat the reagent in the second reagent needle 132 alone, or heat the reagents in the first reagent needle 131 and the second reagent needle 132. Preferably, the first reagent needle 131 and the second reagent needle 132 have a heating function, that is, the heating mechanism comprises a first heating component and a second heating component, the first heating component is arranged in the first reagent needle 131 and used for heating the reagent in the first reagent needle 131, and the second heating component is arranged in the second reagent needle 132 and used for heating the reagent in the second reagent needle 132. After the first reagent needle 131 and the second reagent needle 132 absorb the reagent from the reagent storage mechanism, the reagent is stored in the first reagent needle 131 and the second reagent needle 132, the first reagent needle 131 heats the reagent through the first heating component in the first reagent needle 131, the second reagent needle 132 heats the reagent through the second heating component in the second reagent needle 132, and after the temperature of the reagent reaches a preset temperature, the reagent is added to the reaction cup of the sample incubation device 300 or the reaction cup of the sample detection device 400, so as to accelerate the reaction speed of the sample and the reagent and improve the analysis efficiency of the sample analysis equipment. Moreover, the first heating component and the second heating component can quickly and accurately heat the reagents in the first reagent needle 131 and the second reagent needle 132 and maintain the reagents at a preset temperature, so as to ensure the accuracy and efficiency of the test results.

[0086] Preferably, after the first reagent needle 131 completes the absorption of the reagent, the driving mechanism 110 drives the fixed frame 120 to rotate, and the first reagent needle 131 can heat the reagent therein at any position. At the same time, the second reagent needle 132 can rotate to the reagent absorption position of the reagent storage device 200 to absorb the reagent, rotate to the first reagent adding position of the sample incubation device 300 to add the reagent, rotate to the second reagent adding position of the sample detection device 400 to discharge the reagent, or heat the reagent in the second reagent needle 132. That is, when the first reagent needle 131 performs the heating operation, the second reagent needle 132 can perform one or more of the operations of absorbing the reagent, heating the reagent, and discharging the reagent. Similarly, when the second reagent needle 132 heats the reagent therein, the first reagent needle 131 can also perform the above operations. That is, when the second reagent needle 132 performs the heating operation, the first reagent needle 131 can perform one or more of the operations of absorbing the reagent, heating the reagent, and discharging the reagent.

[0087] The reagent adding device 100 of the present invention uses a first reagent needle 131 and a second reagent needle 132 to realize the transfer of reagents, so that when the first reagent needle 131 performs the heating operation, the second reagent needle 132 performs the operations of sucking reagents, heating reagents, and discharging reagents. When one of the reagent needles is heated, the other reagent needle can perform one or more of the operations of sucking reagents, heating reagents, and discharging reagents, thereby improving the utilization rate of the driving mechanism 110, thereby improving the efficiency of reagent transfer, improving the efficiency of the sample analysis equipment, and making the sample analysis equipment perform better.

[0088] Preferably, the angle between the line connecting the first fixed end and the center of the fixing frame 120 and the line connecting the second fixed end and the center of the fixing frame 120 is in the range of 30° to 180°. In other words, the angle between the first rocker arm 121 and the second rocker arm 122 is in the range of 30° to 180°. Figure 1 The arrow shown. In this way, when the first reagent needle 131 is performing the operation of absorbing or discharging the reagent, the second reagent needle 132 will deviate from the reagent absorbing station, the first reagent adding station or the second reagent adding station, thereby preventing the second reagent needle 132 from touching the reagent storage device 200, the sample incubation device 300 and the sample detection device 400, improving the reliability of the operation of the reagent adding device 100, and thus ensuring the performance of the sample analysis equipment. Moreover, when the first reagent needle 131 is performing the operation of absorbing the reagent, heating the reagent and discharging the reagent, the second reagent needle 132 can heat the reagent therein at the other end, so that one reagent needle can continue to work while the other reagent needle is performing the heating operation, and the resource utilization rate is greatly improved, thereby improving the working efficiency of the sample analysis equipment.

[0089] In this embodiment, the line connecting the first fixed end and the center of the fixing frame 120 and the second fixed end and a point on the centerline of the fixing frame 120 are collinear. That is, the first rocker arm 121 and the second rocker arm 122 are collinearly arranged in a horizontal plane, and the first fixed end and the second fixed end are respectively located at the farthest ends of the collinear relationship between the first rocker arm 121 and the second rocker arm 122. That is, the distance between the first reagent needle 131 and the second reagent needle 132 is the length of the first rocker arm 121 and the second rocker arm 122. In this way, the space in which the second reagent needle 132 is located when the first reagent needle 131 is performing the reagent aspirating and dispensing operations is completely consistent with the space in which the first reagent needle 131 is located when the second reagent needle 132 is performing the reagent aspirating and dispensing operations. This facilitates the design of the clearance space, optimizes the overall structure of the sample analysis device, makes the positions of the first reagent needle 131 and the second reagent needle 132 more controllable, and facilitates the control of the reagent adding device 100.

[0090] As an implementation manner, the driving mechanism 110 comprises a rotating shaft 111, a first driving assembly 112 and a second driving assembly 113, the fixed frame 120 is arranged on the rotating shaft 111, the first driving assembly 112 is mounted on the second driving assembly 113, and the first driving assembly 112 and the second driving assembly 113 are connected with the rotating shaft 111, the first driving assembly 112 is used for driving the rotating shaft 111 to rotate, and the second driving assembly 113 is used for driving the rotating shaft 111 to move up and down. The rotating shaft 111 is connected with the fixed frame 120, that is, the first rocker arm 121 and the second rocker arm 122 are respectively mounted on the rotating shaft 111, the first driving assembly 112 drives the rotating shaft 111 to rotate, and then the rotating shaft 111 drives the first rocker arm 121 and the second rocker arm 122 to rotate, so that the first reagent needle 131 and the second reagent needle 132 rotate to the corresponding positions of the sample analysis equipment and perform corresponding operations. The second driving assembly 113 drives the rotating shaft 111 to move up and down, and then the rotating shaft 111 drives the first rocker arm 121 and the second rocker arm 122 to move up and down; when the second driving assembly 113 drives the rotating shaft 111 to drive the first reagent needle 131 and the second reagent needle 132 to move downward, the first reagent needle 131 and the second reagent needle 132 can perform the operations of sucking or spitting reagents, after completion, the second driving assembly 113 drives the rotating shaft 111 to drive the first reagent needle 131 and the second reagent needle 132 to move upward; then, the first driving assembly 112 drives the rotating shaft 111 to drive the first reagent needle 131 and the second reagent needle 132 to rotate to the corresponding positions to perform corresponding operations.

[0091] Of course, in other embodiments of the present application, the driving mechanism 110 can also drive the first reagent needle 131 and the second reagent needle 132 to rotate alone. That is, when the driving mechanism 110 drives the first reagent needle 131 to rotate to perform one or more of the operations of sucking reagents, heating reagents and spitting reagents, the second reagent needle 132 is in a static state to heat reagents; on the contrary, when the driving mechanism 110 drives the second reagent needle 132 to rotate to perform one or more of the operations of sucking reagents, heating reagents and spitting reagents, the first reagent needle 131 is in a static state to heat reagents.

[0092] Specifically, the first driving assembly 112 comprises a first driving motor 1121 and a first transmission component 1122. The first driving motor 1121 is mounted on the support frame 140, and the first transmission component 1122 is mounted on the first driving motor 1121 and the rotating shaft 111. The first transmission component 1122 is in transmission connection between the output shaft of the first driving motor 1121 and the rotating shaft 111, so as to drive the rotating shaft 111 to rotate, and then the rotating shaft 111 drives the first reagent needle 131 and the second reagent needle 132 on the fixed frame 120 to rotate. The second driving assembly 113 comprises a second driving motor 1131 and a second transmission component 1132. The second driving motor 1131 is mounted on the support frame 140, and the second transmission component 1132 is mounted on the second driving motor 1131 and the rotating shaft 111. The second transmission component 1132 is in transmission connection between the output shaft of the second driving motor 1131 and the rotating shaft 111, so as to drive the rotating shaft 111 to move up and down, and then the rotating shaft 111 drives the first reagent needle 131 and the second reagent needle 132 on the fixed frame 120 to move up and down. Optionally, the first transmission component 1122 and the second transmission component 1132 can be a synchronous belt transmission structure, and of course, can also be a chain transmission structure and other structures capable of achieving motion transmission.

[0093] The specific process of transferring reagent by the first reagent needle 131 is as follows: when the first reagent needle 131 transfers reagent, the first driving assembly 112 drives the rotating shaft 111 to drive the first rocker arm 121 and the second rocker arm 122 to rotate, so that the first rocker arm 121 drives the first reagent needle 131 to rotate to the reagent suction position of the reagent storage device 200; then, the second driving assembly 113 drives the rotating shaft 111 to drive the first rocker arm 121 to descend, and then the first rocker arm 121 drives the first reagent needle 131 to descend, at this time, the first reagent needle 131 sucks reagent at the reagent suction position; after the reagent is sucked, the second driving assembly 113 drives the rotating shaft 111 to drive the first rocker arm 121 to ascend, and then the first rocker arm 121 drives the first reagent needle 131 to ascend; then, the first reagent needle 131 heats the reagent therein by the first heating component, and when the temperature of the first reagent needle 131 reaches the preset temperature, the first reagent needle 131 can perform the reagent adding operation, at this time, the first driving assembly 112 drives the rotating shaft 111 to drive the first rocker arm 121 to rotate, and then the first rocker arm 121 drives the first reagent needle 131 to rotate to the first reagent adding position of the sample incubation device 300 or to the third reagent adding position of the magnetic bead method detection mechanism. When the first reagent needle 131 rotates to the first reagent adding position of the sample incubation device 300, the second driving assembly 113 drives the rotating shaft 111 to drive the first rocker arm 121 to descend, and then the first rocker arm 121 drives the first reagent needle 131 to descend, and the first reagent needle 131 spits reagent into the reaction cup of the sample incubation module at the first reagent adding position; after the reagent is added, the second driving assembly 113 drives the rotating shaft 111 to drive the first rocker arm 121 to ascend, and then the first rocker arm 121 drives the reagent needle to ascend. When the first reagent needle 131 rotates to the third reagent adding position of the magnetic bead method detection mechanism, the second driving assembly 113 drives the rotating shaft 111 to drive the first rocker arm 121 to descend, and then the first rocker arm 121 drives the first reagent needle 131 to descend, and the first reagent needle 131 spits reagent into the reaction cup of the magnetic bead method detection mechanism at the third reagent adding position; after the reagent is added, the second driving assembly 113 drives the rotating shaft 111 to drive the first rocker arm 121 to ascend, and then the first rocker arm 121 drives the first reagent needle 131 to ascend.

[0094] It should be noted that the working process and position of the second reagent needle 132 are completely same as those of the first reagent needle 131. When the first reagent needle 131 is heated by the first heating component, the first driving assembly 112 drives the rotating shaft 111 to rotate the second reagent needle 132 to the reagent suction station, the first reagent adding station or the second reagent adding station to perform the same operation as the first reagent needle 131, at this time, the position of the first reagent needle 131 does not need to be controlled. When the second reagent needle 132 is heated by the second heating component, after the reagent in the first reagent needle 131 is heated, the first driving assembly 112 drives the first reagent needle 131 to rotate to the first reagent adding station or the second reagent adding station to perform the corresponding operation, at this time, the position of the second reagent needle 132 does not need to be controlled. Such a cycle operation realizes the transfer of the reagent and improves the operation efficiency.

[0095] Moreover, the order of adding the sample and the reagent in the sample incubation device 300 is not limited. The sample can be added in the reaction cup first, and then the reagent is ejected from the reagent adding device 100; of course, the reagent can also be ejected from the reagent adding device 100 first, and then the sample is added. At this time, the reagent adding device 100 of the present application can transfer the reagent from the reagent storage device 200 to the reaction cup with the sample in the sample incubation device 300, or the reagent can also be added to the empty reaction cup in the sample incubation device 300. When used, the corresponding reaction cup can be selected according to the actual use requirement.

[0096] Optionally, the reagent adding device 100 further comprises a control mechanism 150, the control mechanism 150 comprises a first temperature control mainboard 151 and a second temperature control mainboard 152, and the first temperature control mainboard 151 and the second temperature control mainboard 152 are arranged on the fixed frame 120. The first temperature control mainboard 151 is electrically connected with the first heating component, and is used for controlling the heating temperature of the reagent by the first heating component; the second temperature control mainboard 152 is electrically connected with the second heating component, and is used for controlling the heating temperature of the reagent by the second heating component. The first temperature control mainboard 151 and the second temperature control mainboard 152 can ensure that the reagent in the first reagent needle 131 and the second reagent needle 132 is quickly and accurately heated and maintained at a preset temperature, thereby ensuring the accuracy and efficiency of the test result. Preferably, the first heating component and the second heating component can be heating wires, of course, the first heating component and the second heating component can also be other structures capable of realizing the heating function.

[0097] Further, the control mechanism 150 further comprises a first detection mainboard 153 and a second detection mainboard 154, both of which are arranged on the fixed frame 120. The first detection mainboard 153 is electrically connected with the first reagent needle 131, and is used to detect the liquid level in the reagent bottle from which the first reagent needle 131 sucks reagent. The second detection mainboard 154 is electrically connected with the second reagent needle 132, and is used to detect the liquid level in the reagent bottle from which the second reagent needle 132 sucks reagent. In this way, the control mechanism 150 can control the distance of downward movement of the first reagent needle 131 and the second reagent needle 132, i.e. the depth of penetration of the first reagent needle 131 and the second reagent needle 132, according to the height of the liquid level in the reagent bottle. At the same time, when the liquid level in the reagent bottle is too low, the control mechanism 150 can also prompt timely replacement of the reagent bottle or addition of reagent to the reagent bottle, so as to ensure normal operation of the equipment. Optionally, the first reagent needle 131 is provided with a first liquid level detection component, which is electrically connected with the first detection mainboard 153 and is used to determine the liquid level at the end of the first reagent needle 131. The second reagent needle 132 is provided with a second liquid level detection component, which is electrically connected with the second detection mainboard 154 and is used to determine the liquid level at the end of the second reagent needle 132. Preferably, the first liquid level detection component and the second liquid level detection component can be sensors, and of course can also be other structures capable of detecting liquid level.

[0098] Moreover, the first temperature control mainboard 151 and the first detection mainboard 153 are arranged opposite to each other on the first rocker arm 121 of the fixed frame 120, and a space exists between the first temperature control mainboard 151 and the first detection mainboard 153, which facilitates routing of other pipelines, wires, etc. of the first reagent needle 131, saves space, and ensures that the structure is neat and orderly. The second temperature control mainboard 152 and the second detection mainboard 154 are arranged opposite to each other on the second rocker arm 122 of the fixed frame 120, and a space exists between the second temperature control mainboard 152 and the second detection mainboard 154, which facilitates routing of other pipelines, wires, etc. of the second reagent needle 132, saves space, and ensures that the structure is neat and orderly.

[0099] Referring to Figure 3 , Figure 3A liquid path diagram of the first reagent needle 131 and the second reagent needle 132 in the reagent adding device 100 of an embodiment of the present application. Optionally, the reagent adding device 100 further comprises a cleaning mechanism 160. The cleaning mechanism 160 is used to clean the first reagent needle 131 and the second reagent needle 132. In order to avoid the first reagent needle 131 and the second reagent needle 132 from contaminating the sample when transferring the reagent, the first reagent needle 131 and the second reagent needle 132 need to be cleaned after each transfer of the reagent, to avoid mutual infection between the sample and the reagent and mutual infection between different reagents, and to ensure the reliability of the sample analysis equipment detection. Specifically, the cleaning mechanism 160 comprises a cleaning main pipe 161, a first cleaning branch pipe 162 and a second cleaning branch pipe 163, and the cleaning main pipe 161, the first cleaning branch pipe 162 and the second cleaning branch pipe 163 are all arranged on the fixed frame 120. One end of the first cleaning branch pipe 162 is in communication with the first reagent needle 131, and the other end of the first cleaning branch pipe 162 is in communication with the cleaning main pipe 161; one end of the second cleaning branch pipe 163 is in communication with the second reagent needle 132, and the other end of the second cleaning branch pipe 163 is in communication with the cleaning main pipe 161. The first cleaning branch pipe 162 is used to clean the first reagent needle 131, and the second cleaning branch pipe 163 is used to clean the second reagent needle 132. The end of the cleaning main pipe 161 away from the first cleaning branch pipe 162 is connected to a liquid source, and the liquid source stores cleaning liquid. The cleaning main pipe 161 can deliver the cleaning liquid in the liquid source to the first reagent needle 131 through the first cleaning branch pipe 162 and to the second reagent needle 132 through the second cleaning branch pipe 163. Moreover, the first cleaning branch pipe 162 and the second cleaning branch pipe 163 share the cleaning main pipe 161, and the cleaning main pipe 161 provides cleaning liquid to the first cleaning branch pipe 162 and the second cleaning branch pipe 163 respectively, so that the cleaning liquid is delivered to the first reagent needle 131 and the second reagent needle 132 respectively, to achieve the purpose of cleaning the first reagent needle 131 and the second reagent needle 132, while also reducing the number of pipelines and thereby reducing the complexity of the structure. Moreover, the first cleaning branch pipe 162 is arranged in the space between the first temperature control main board 151 and the first detection main board 153, the second cleaning branch pipe 163 is arranged in the space between the second temperature control main board 152 and the second detection main board 154, and the cleaning main pipe 161 is arranged in the rotating shaft 111.

[0100] Further, the cleaning main pipe 161 is provided with a cleaning main valve 164, the first cleaning branch pipe 162 is provided with a first cleaning branch valve 165, and the first cleaning branch valve 165 is located between the cleaning main valve 164 and the first reagent needle 131. The second cleaning branch pipe 163 is provided with a second cleaning branch valve 166, and the second cleaning branch valve 166 is located between the cleaning main valve 164 and the second reagent needle 132. The cleaning main valve 164 is used to control the opening and closing of the cleaning main pipe 161, the first cleaning branch valve 165 is used to control the opening and closing of the first cleaning branch pipe 162, and the second cleaning branch valve 166 is used to control the opening and closing of the second cleaning branch pipe 163. When the first cleaning branch valve 165 and the second cleaning branch valve 166 are opened and the cleaning main valve 164 is closed, the first reagent needle 131 is controlled to be cleaned. When the first reagent needle 131 is controlled to be cleaned, the second cleaning branch valve 166 is closed, the cleaning main valve 164 and the first cleaning branch valve 165 are opened, and the cleaning liquid enters the first reagent needle 131 through the cleaning main pipe 161 and the first cleaning branch pipe 162 to perform the cleaning operation. After the cleaning is completed, the cleaning main valve 164 is closed, and the first cleaning branch valve 165 is closed after the cleaning liquid is completely drained.

[0101] In addition, the cleaning mechanism 160 further comprises a pump 167, which is arranged on the cleaning main pipe 161 and located between the cleaning main valve 164 and the first cleaning branch valve 165 and the second cleaning branch valve 166. For example, when the first cleaning branch valve 165 is opened and the cleaning main valve 164 and the second cleaning branch valve 166 are closed, the pump 167 is used to control the first reagent needle 131 to suck or spit the reagent. When the first reagent needle 131 is controlled to suck or spit the reagent, the cleaning main valve 164 and the second cleaning branch valve 166 are closed, and the first cleaning branch valve 165 is opened. The pump 167 is used to suck or spit the reagent. When the reagent is sucked, the reagent is stored in the first reagent needle 131. Further, the cleaning main valve 164 and the first cleaning branch valve 165 are closed to control the first reagent needle 131 to heat the reagent. When the first reagent needle 131 heats the reagent, the cleaning main valve 164 and the first cleaning branch valve 165 are closed to prevent the reagent from flowing into the cleaning main pipe 161 or flowing out of the first reagent needle 131.

[0102] Referring to Figure 4 and Figure 5 , Figure 4 the working flowchart of the reagent adding device 100 according to an embodiment of the present application, Figure 5 is Figure 4 the working flowchart of the first reagent needle 131 and the second reagent needle 132. The present application further provides a control method of the reagent adding device 100, which is applied to the reagent adding device 100 according to the above-mentioned embodiments. The control method of the reagent adding device 100 comprises the following steps:

[0103] Step S1: the fixed frame 120 of the reagent adding device 100 drives the first reagent needle 131 and the second reagent needle 132 to perform lifting and rotating movements, the first reagent needle 131 performs cleaning, reagent suction, reagent heating and reagent discharge operations, and the second reagent needle 132 performs cleaning, reagent suction, reagent heating and reagent discharge operations.

[0104] Step S2: when the reagent in the first reagent needle 131 is heated, the second reagent needle 132 rotates to the corresponding position to perform one or more combined operations of cleaning, reagent suction, reagent heating and reagent discharge.

[0105] When the reagent is transferred, the driving mechanism 110 of the reagent adding device 100 drives the fixed frame 120 to drive the first reagent needle 131 and the second reagent needle 132 to perform lifting and rotating movements, so that the first reagent needle 131 and the second reagent needle 132 perform cleaning, reagent suction, reagent heating and reagent discharge operations. The operation process of the first reagent needle 131 and the second reagent needle 132 performing the above operations has been described above, and will not be repeated here.

[0106] It can be understood that before the reagent is sucked, the first reagent needle 131 usually performs a cleaning operation, after the cleaning is completed, the driving mechanism 110 drives the first reagent needle 131 to rotate to the reagent suction station and suck the reagent; when the first reagent needle 131 sucks the reagent and heats the reagent therein, the driving mechanism 110 can drive the fixed frame 120 to rotate, so that the second reagent needle 132 rotates to the cleaning station to perform a cleaning operation, rotates to the reagent suction station of the reagent storage device 200 to suck the reagent, or the second reagent needle 132 rotates to the first reagent adding station of the sample incubation device 300 or the second reagent adding station of the sample detection device 400, or the second reagent needle 132 also performs corresponding heating operation after sucking the reagent. Of course, the second reagent needle 132 can also perform a combination of cleaning, reagent suction, reagent heating and reagent discharge operations.

[0107] Optionally, when the reagent in the second reagent needle 132 is heated, the first reagent needle 131 rotates to the corresponding position to perform one or more combined operations of cleaning, sucking reagent, heating reagent and discharging reagent. When the reagent in the second reagent needle 132 is heated after being sucked by the second reagent needle 132, the driving mechanism 110 can drive the first reagent needle 131 to rotate to the cleaning station to perform cleaning operation, can drive the first reagent needle 131 to rotate to the reagent sucking station of the reagent storage device 200 to suck reagent, can drive the first reagent needle 131 to rotate to the first reagent adding station of the sample incubation device 300 or the second reagent adding station of the sample detection device 400, can drive the first reagent needle 131 to perform corresponding heating operation after sucking reagent, and can drive the first reagent needle 131 to perform combination of multiple operations of cleaning, sucking reagent, heating reagent and discharging reagent. For example, the cleaning operation and the reagent sucking operation are performed; or the reagent sucking operation, the reagent heating operation, the reagent discharging operation and the cleaning operation are performed; or the cleaning operation, the reagent sucking operation, the reagent heating operation and the reagent discharging operation are performed, and so on.

[0108] Further, when the second reagent needle 132 performs multiple combined operations, the second reagent needle 132 is cyclically performed according to the arrangement order of cleaning, sucking reagent, heating reagent and discharging reagent.

[0109] That is, the operation performed by the second reagent needle 132 is cyclically performed according to the order of cleaning, sucking reagent, heating reagent and discharging reagent; and when the first reagent needle 131 performs multiple combined operations, the first reagent needle 131 is also cyclically performed according to the arrangement order of cleaning, sucking reagent, heating reagent and discharging reagent.

[0110] In one specific embodiment of the present application, step S10: the driving mechanism 110 drives the first reagent needle 131 to rotate to the cleaning station and performs cleaning operation; after the first reagent needle 131 is cleaned, step S11 is performed, the driving mechanism 110 drives the first reagent needle 131 to rotate to the reagent suction station of the reagent storage device 200 to suck reagent; after the suction is completed, step S12 is performed, the first reagent needle 131 heats the reagent therein, and at the same time, step S13 is performed, the driving mechanism 110 drives the second reagent needle 132 to rotate to the cleaning station; after the second reagent needle 132 is cleaned, step S14 is performed, the driving mechanism 110 drives the second reagent needle 132 to rotate to the reagent suction station of the reagent storage device 200 to suck reagent; after the second reagent needle 132 sucks the reagent, step S15 is performed, the second reagent needle 132 heats the reagent therein; at the same time, after the reagent in the first reagent needle 131 is heated to the required temperature, step S16 is performed, the driving mechanism 110 drives the first reagent needle 131 to rotate to the first reagent adding station of the sample incubation device 300 or the second reagent adding station of the sample detection device 400; after the first reagent needle 131 discharges the reagent therein, step S17 is performed, the driving mechanism 110 drives the first reagent needle 131 to rotate to the cleaning station to perform cleaning operation; after the cleaning is completed, when the temperature of the reagent in the second reagent needle 132 is heated to the required temperature, step S18 is performed, the driving mechanism 110 drives the second reagent needle 132 to rotate to the first reagent adding station of the sample incubation device 300 or the second reagent adding station of the sample detection device 400; the second reagent needle 132 discharges the reagent therein, step S19 is performed, and then the driving mechanism 110 drives the second reagent needle 132 to rotate to the cleaning station to perform cleaning operation; when the reagent in the second reagent needle 132 still needs to be heated, at this time, the driving mechanism 110 can drive the first reagent needle 131 to perform the operations of sucking reagent and heating reagent, and steps S10 to S12 are performed. When the reagent adding device 100 repeatedly transfers reagent, the first reagent needle 131 and the second reagent needle 132 are sequentially and circularly executed according to steps S10 to S19, so that the first reagent needle 131 and the second reagent needle 132 are used alternately. It should be noted that when the reagent in the second reagent needle 132 is heated, the sequential execution of several steps by the first reagent needle 131 is determined by the heating time of the second reagent needle 132. For example, when the heating time of the second reagent needle 132 is short, the first reagent needle 131 can only perform cleaning operation or cleaning and reagent suction operation, etc.; when the heating time of the second reagent needle 132 is long, the first reagent needle 131 can perform cleaning, reagent suction and reagent heating operations or cleaning, reagent suction, reagent heating and reagent discharge operations.

[0111] In this way, the first reagent needle 131 can perform the operations of cleaning, sucking reagent, heating reagent and discharging reagent while the second reagent needle 132 is heating reagent therein, and the second reagent needle 132 can perform the operations of cleaning, sucking reagent, heating reagent and discharging reagent while the first reagent needle 131 is heating reagent therein, so that one of the reagent needles can perform other operations while the other reagent needle is performing the operation of heating reagent, that is, the other reagent needle can perform various actions, so that the first reagent needle 131 and the second reagent needle 132 will not be in an idle state, the utilization rate of the driving mechanism 110 is improved, the resource utilization rate is greatly improved, and the working efficiency of the sample analysis equipment is improved.

[0112] Of course, in other embodiments of the application, the first reagent needle 131 and the second reagent needle 132 can also perform sequentially, that is, after the first reagent needle 131 sequentially performs the operations of cleaning, sucking reagent, heating reagent and discharging reagent, the second reagent needle 132 sequentially performs the operations of cleaning, sucking reagent, heating reagent and discharging reagent. In this way, the transfer of reagents can also be achieved.

[0113] Referring to Figure 3 and Figure 6 , Figure 6 is a workflow diagram for the operation of the first reagent needle 131. As a possible embodiment, when the first reagent needle 131 performs the cleaning operation, the cleaning liquid path of the first reagent needle 131 is connected, and after the cleaning is completed, the cleaning liquid path is disconnected;

[0114] When the first reagent needle 131 performs the operation of sucking reagent, the suction and discharge liquid path of the first reagent needle 131 is connected, and after the operation of sucking reagent is completed, the suction and discharge liquid path is disconnected;

[0115] When the first reagent needle 131 performs the operation of discharging reagent, the suction and discharge liquid path of the first reagent needle 131 is connected, and after the operation of discharging reagent is completed, the suction and discharge liquid path is disconnected.

[0116] The connection of the suction and discharge liquid path of the first reagent needle 131 refers to the first cleaning branch pipe 162 of the first reagent needle 131 and the first cleaning branch valve 165 thereon, and the first cleaning branch valve 165 controls the connection and disconnection of the first cleaning branch pipe 162; and the cleaning liquid path includes the suction and discharge liquid path and the cleaning main pipe 161 connected to the suction and discharge liquid path. During cleaning, the cleaning liquid path provides cleaning liquid to the suction and discharge liquid path through the cleaning main pipe 161, and after cleaning is completed, the cleaning main pipe 161 of the cleaning liquid path is closed, and the cleaning operation is completed. When the first reagent needle 131 sucks or discharges reagent, the suction and discharge liquid path can facilitate the first reagent needle 131 to suck or discharge reagent. When heating, the suction and discharge reagent is in a closed state to avoid liquid leakage.

[0117] The opening and closing of the cleaning liquid path and the suction and spitting liquid path of the first reagent needle 131 will be described by taking the first reagent needle 131 as an example. When the first reagent needle 131 performs the cleaning operation, step S30 is performed, the first cleaning branch valve 165 is opened, the second cleaning branch valve 166 is closed, then step S31 is performed, the cleaning main valve 164 is opened, step S32 is performed, the cleaning liquid is supplied to the first reagent needle 131 to clean the first reagent needle 131; after the cleaning is completed, step S33 is performed, the cleaning main valve 164 is closed, at this time, the first cleaning branch valve 165 is in the open state, the second cleaning branch valve 166 is in the closed state, step S34 is performed, the first reagent needle 131 sucks the reagent, after the sucking is completed, step S35 is performed, the first cleaning branch valve 165 is closed. Then, step S36 is performed, the first reagent needle 131 performs the heating operation; after the heating is completed, step S37 is performed, the first cleaning branch valve 165 is opened, the second cleaning branch valve 166 is still in the closed state, step S38 is performed, the first reagent needle 131 performs the operation of spitting the reagent. Then, the cleaning main valve 164 is opened, the cleaning liquid is supplied to the first reagent needle 131 to clean the first reagent needle 131; after the cleaning is completed, the cleaning main valve 164 is closed. In this way, the cleaning, sucking reagent, heating reagent and spitting reagent operations of the first reagent needle 131 are repeatedly performed. When the reagent adding device 100 stops working, after the first reagent needle 131 is cleaned, the cleaning main valve 164 is closed, and then the first cleaning branch valve 165 is closed. It should be noted that the control mode of the second reagent needle 132 is the same as that of the first reagent needle 131, and thus will not be described here.

[0118] Referring to Figure 1 and Figure 2 , the present application also provides a sample analysis device, comprising a sample incubation device 300, a reagent storage device 200, a sample detection device 400 and the reagent adding device 100 in the above embodiment. The sample incubation device 300 and the reagent storage device 200 are arranged side by side, the sample detection device 400 is arranged on one side of the sample incubation device 300 and the reagent storage device 200, and the reagent adding device 100 is arranged between the sample incubation device 300, the reagent storage device 200 and the sample detection device 400. The reagent adding device 100 can transfer the reagent in the reagent storage device 200 to the sample incubation device 300 or the sample detection device 400. The reagent is transferred between the sample incubation device 300, the reagent storage device 200 and the sample detection device 400 by the reagent adding device 100, so that the sample analysis device can detect the sample. The sample analysis device further comprises a transfer device and a stirring device. The transfer device is used to transfer the reaction cup, transfer the empty reaction cup to the sample incubation device 300, and transfer the reaction cup containing the sample and the reagent to the sample detection device 400. The stirring device is used to stir the sample and the reagent in the reaction cup, so that the sample and the reagent are uniformly mixed, and the detection result is accurate.

[0119] Further, when one of the reagent needles of the reagent adding device 100 is sucking reagent in the reagent storage device 200, the space where the other reagent needle is located is the first avoidance space. When one of the reagent needles of the reagent adding device 100 is discharging reagent on the sample incubation device 300, the space where the other reagent needle is located is the second avoidance space. When one of the reagent needles of the reagent adding device 100 is discharging reagent on the sample detection device 400, the space where the other reagent needle is located is the third avoidance space. When one of the reagent needles of the reagent adding device 100 is cleaning, the space where the other reagent needle is located is the fourth avoidance space.

[0120] Since the reagent adding device 100 is a double-needle structure, the reagent adding device 100 may interfere with the transfer device, the stirring device, the sample incubation device 300, the reagent storage device 200 and other components during the transfer of reagent, resulting in downtime of the sample analysis equipment, so it is necessary to limit the avoidance space of the first reagent needle 131 and the second reagent needle 132. When the first reagent needle 131 performs an operation, the second reagent needle 132 should be in the avoidance space. Correspondingly, when the second reagent needle 132 performs an operation, the first reagent needle 131 should also be in the avoidance space, to ensure the reliable operation of the reagent adding device 100 and improve the reliability of the sample analysis equipment. Specifically, when the first reagent needle 131 is sucking reagent in the reagent taking station, the space where the second reagent needle 132 is located is the first avoidance space. When the first reagent needle 131 is sucking reagent in the reagent taking station of the reagent storage device 200, the second reagent needle 132 has an avoidance space at position A shown by 2, and the transfer device needs to avoid the second reagent needle 132 to avoid collision and interference between the second reagent needle 132 and other components during lifting. When the first reagent needle 131 is adding reagent in the first reagent adding station, the space where the second reagent needle 132 is located is the second avoidance space. When the first reagent needle 131 is adding reagent in the first reagent adding station on the sample incubation device 300, the second reagent needle 132 has an avoidance space at position B shown by 2, to avoid collision and interference between the second reagent needle 132 and other components during lifting. Figure 2 When the first reagent needle 131 is adding reagent in the first reagent adding station, the space where the second reagent needle 132 is located is the third avoidance space. When the first reagent needle 131 is adding reagent in the second reagent adding station on the sample detection device 400, the second reagent needle 132 has an avoidance space at position C shown by 2, to avoid collision and interference between the second reagent needle 132 and other components during lifting. Figure 2 When the first reagent needle 131 is cleaning, the space where the second reagent needle 132 is located is the fourth avoidance space. When the first reagent needle 131 is cleaning, the second reagent needle 132 has an avoidance space at position D shown by 2, to avoid collision and interference between the second reagent needle 132 and other components during lifting. Figure 2The B shown has an avoiding space to avoid collision and interference between the second reagent needle 132 and other components when lifting.

[0121] Optionally, the sample analysis device further comprises a waterproof component (not shown), the sample detection device 400 comprises a magnetic bead detection mechanism, and the waterproof component is arranged between the magnetic bead detection mechanism and the reagent storage device 200. In order to avoid the reagent drops on the surfaces of the first reagent needle 131 and the second reagent needle 132 from falling on the control mainboard of the magnetic bead detection mechanism, the waterproof component is arranged between the magnetic bead detection mechanism and the reagent storage device 200, and the waterproof component plays a water-blocking role to avoid water drops.

[0122] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description simple, all possible combinations of the technical features in the above-mentioned embodiments are not described, but as long as the combinations of the technical features do not exist, they should be considered as the description range of the present application.

[0123] The above-mentioned embodiments only express several implementation manners of the present application, the description is more specific and detailed, but it should not be understood as the limitation of the patent scope of the present application. It should be pointed out that for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application should be subject to the appended claims.

Claims

1. A reagent adding device, characterized in that: include: A driving mechanism, wherein the driving structure is used to drive the reagent needle mechanism to move; a fixing frame connected to the driving mechanism, the fixing frame comprising a first fixing end and a second fixing end; The reagent needle mechanism includes a first reagent needle and a second reagent needle, wherein the first reagent needle is arranged on the first fixed end, and the second reagent needle is arranged on the second fixed end; a heating mechanism, for heating at least one of the first reagent needle and the second reagent needle, wherein the heating mechanism is disposed in the first reagent needle and / or the second reagent needle; a cleaning mechanism, for cleaning the first reagent needle and the second reagent needle; The first reagent needle can perform cleaning, reagent aspiration, reagent heating and reagent spitting operations; the second reagent needle can perform cleaning, reagent aspiration, reagent heating and reagent spitting operations; when one of the first reagent needle and the second reagent needle performs the heating operation, the other can perform one or more combined operations of cleaning, reagent aspiration, reagent heating and reagent spitting.

2. The reagent adding device according to claim 1, characterized in that: An included angle between a line connecting the first fixing end and the center of the fixing frame and a line connecting the second fixing end and the center of the fixing frame ranges from 30° to 180°.

3. The reagent adding device according to claim 2, characterized in that: An angle between a line connecting the first fixing end and the center of the fixing frame and a line connecting the second fixing end and the center of the fixing frame is 180°.

4. The reagent adding device according to any one of claims 1 to 3, characterized in that: The heating mechanism includes a first heating component and a second heating component. The first heating component is used to heat the reagent in the first reagent needle; the second heating component is used to heat the reagent in the second reagent needle.

5. The reagent adding device according to claim 4, characterized in that: The reagent adding device further includes a control mechanism, the control mechanism including a first temperature control mainboard and a second temperature control mainboard, the first temperature control mainboard and the second temperature control mainboard are respectively arranged on the fixing frame; The first temperature control mainboard is electrically connected to the first heating component for controlling the heating temperature of the reagent by the first heating component; the second temperature control mainboard is electrically connected to the second heating component for controlling the heating temperature of the reagent by the second heating component.

6. The reagent adding device according to claim 5, characterized in that: The control mechanism further includes a first detection mainboard and a second detection mainboard, wherein the first detection mainboard and the second detection mainboard are both arranged on the fixing frame; The first detection mainboard is used to detect the liquid level height in the reagent bottle through which the first reagent needle draws reagent; the second detection mainboard is used to detect the liquid level height in the reagent bottle through which the second reagent needle draws reagent.

7. The reagent adding device according to any one of claims 1 to 3, characterized in that: The cleaning mechanism includes a cleaning main pipe, a first cleaning branch pipe and a second cleaning branch pipe; One end of the first cleaning branch pipe is connected to the first reagent needle, and the other end of the first cleaning branch pipe is connected to the cleaning main pipe; one end of the second cleaning branch pipe is connected to the second reagent needle, and the other end of the second cleaning branch pipe is connected to the cleaning main pipe.

8. The reagent adding device according to claim 7, characterized in that: The cleaning main pipe is provided with a cleaning main valve, the first cleaning branch pipe is provided with a first cleaning branch valve, and the second cleaning branch pipe is provided with a second cleaning branch valve; wherein, The cleaning main valve and the first cleaning branch valve are opened, and the second cleaning branch valve is closed to control the cleaning of the first reagent needle; The first cleaning branch valve is opened, and the cleaning main valve and the second cleaning branch valve are closed to control the first reagent needle to absorb or discharge the reagent.

9. The reagent adding device according to claim 8, characterized in that: The main cleaning valve and the first cleaning branch valve are both closed to control the heating of the reagent in the first reagent needle.

10. The reagent adding device according to claim 1, characterized in that: The driving mechanism includes a rotating shaft, a first driving assembly and a second driving assembly. The fixing frame is arranged on the rotating shaft. The first driving assembly is installed on the second driving assembly. The first driving assembly and the second driving assembly are both connected to the rotating shaft. The first driving assembly is used to drive the rotating shaft to rotate, and the second driving assembly is used to drive the rotating shaft to lift.

11. A control method for a reagent adding device, characterized in that: The reagent adding device according to any one of claims 1 to 10 comprises the following steps: Driving the first reagent needle and the second reagent needle to move, the first reagent needle and the second reagent needle perform cleaning, reagent aspiration, reagent heating and reagent discharge operations; When the reagent in the first reagent needle is heated, the second reagent needle rotates to a corresponding position to perform one or more combined operations of cleaning, aspirating the reagent, heating the reagent, and discharging the reagent.

12. The control method of the reagent adding device according to claim 11, characterized in that: When the second reagent needle performs multiple combined operations, the second reagent needle performs the operations cyclically in the order of cleaning, aspirating reagent, heating reagent, and discharging reagent.

13. The control method of the reagent adding device according to claim 11, characterized in that: When the reagent in the second reagent needle is heated, the first reagent needle rotates to a corresponding position to sequentially perform one or more combined operations of discharging the reagent, washing, aspirating the reagent, and heating the reagent.

14. The control method of a reagent adding device according to any one of claims 11 to 13, characterized in that: When the first reagent needle performs a cleaning operation, the cleaning liquid path of the first reagent needle is connected, and after the cleaning is completed, the cleaning liquid path is disconnected; When the first reagent needle performs a reagent aspiration operation, the aspiration and exhalation liquid path of the first reagent needle is connected, and after the reagent aspiration is completed, the aspiration and exhalation liquid path is disconnected; When the first reagent needle performs the reagent discharging operation, the aspiration and discharging liquid path of the first reagent needle is connected, and after the reagent discharging is completed, the aspiration and discharging liquid path is disconnected.

15. A sample analysis device, characterized in that: It comprises a sample incubation device, a reagent storage device, a sample detection device and a reagent adding device according to any one of claims 1 to 10; The sample incubation device and the reagent storage device are arranged side by side, the sample detection device is arranged on one side of the sample incubation device and the reagent storage device, and the reagent adding device is arranged between the sample incubation device, the reagent storage device and the sample detection device. The reagent adding device can transfer the reagent in the reagent storage device to the sample incubation device or the sample detection device.

16. The sample analysis device according to claim 15, characterized in that: When the first reagent needle of the reagent adding device absorbs the reagent in the reagent storage device, the space where the second reagent needle is located is the first avoidance space; When the first reagent needle of the reagent adding device spits out the reagent on the sample incubation device, the space where the second reagent needle is located serves as the second avoidance space; When the first reagent needle of the reagent adding device spits out the reagent from the sample detection device, the space where the second reagent needle is located serves as the third avoidance space; When the first reagent needle of the reagent adding device is being cleaned, the space where the second reagent needle is located serves as a fourth avoidance space.

17. The sample analysis device according to claim 15, characterized in that: The sample analysis equipment further includes a waterproof component. The sample detection device includes a magnetic bead detection mechanism. The waterproof component is provided between the magnetic bead detection mechanism and the reagent storage device.

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