A processing device for extracting genome by boiling method and its use method

Abstract

The present invention relates to the technical field of genome extraction, and in particular to a processing device for genome extraction by boiling method and a method for using the device. The device comprises a bottom plate and a cylinder, wherein a centrifuge is arranged on the bottom plate, the cylinder is fixedly mounted on the bottom plate, a containing tube is movably mounted in the cylinder, a heating rod is arranged in the cylinder, and the rotation of two second stirring rods drives a second connecting ring to rotate. The rotation of the second connecting ring stirs and mixes water in the cylinder, thereby accelerating the speed of heating the water and shortening the time required for heating, thereby accelerating the heating of liquid in the containing tube. The rotation of the first stirring rod stirs and mixes a sample and SDS, thereby fully mixing them, and further accelerates the heating of the liquid in the containing tube, thereby improving the extraction effect of protein variation and DNA in the liquid in a boiling state, and improving the practicality of the processing device.

Description

Technical Field

[0001] The present invention relates to the technical field of genome extraction, and in particular to a processing device for genome extraction by boiling method and a use method thereof. Background Art

[0002] In the field of molecular biology and genetics, a genome is the sum of all the genetic material of an organism, including DNA or RNA (viral RNA).

[0003] For example, a Chinese patent discloses: a new type of cotton genomic DNA extraction device, publication number: CN112553045A, "without a cooling device, the heat generated during the centrifugation process cannot be cooled in time, causing the temperature in the sample tube to rise, exceeding the optimal reaction temperature of the extract and the cotton organ sample, thereby affecting the extraction of the cotton DNA of this variety. After adding an appropriate amount of extract to the ground sample powder, it is necessary to fully shake and mix it, and this process often needs to be completed manually, which is time-consuming, labor-intensive, and inefficient." However, in actual use, the efficiency of water bath heating is low, so it takes a long time to heat the holding tube, reducing the extraction efficiency, and it is inconvenient to take out the holding tube, resulting in inconvenience in cooling the liquid in the holding tube and subsequent processing, which is inconvenient to operate and has poor practicality. Summary of the invention

[0004] In order to overcome the deficiencies of the prior art, the present invention provides a processing device for extracting genomes by boiling method and a method for using the same.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions: A processing device for extracting genomes by boiling method, comprising a bottom plate and a cylinder, wherein a centrifuge is arranged on the bottom plate, the cylinder is fixedly mounted on the bottom plate, a containing tube is movably mounted inside the cylinder, a heating rod is arranged inside the cylinder, a first cover plate is movably mounted on the upper end of the cylinder, three bearings are fixedly mounted on the first cover plate, a stirring structure is arranged inside the cylinder, the stirring structure comprises a motor 1, a second stirring rod and a second cover plate, the motor 1 is fixedly mounted on the upper end of the first cover plate, an output shaft 1 of the motor 1 is fixedly connected to a first stirring rod, three first connecting rings are evenly fixedly mounted on the first stirring rod, each of the first connecting rings is evenly fixedly connected to a first stirring blade, the first connecting ring is movably mounted The second stirring rods penetrate the middle part of the first cover plate, and the number of the second stirring rods is two. The two second stirring rods are symmetrically distributed about the midpoint of the first stirring rod. The first stirring rod is movably arranged in the containing tube. Three second connecting rings are fixedly installed on the two second stirring rods at equal intervals, and each of the second connecting rings is fixedly connected with a second stirring blade. The two second stirring rods movably penetrate the two side ends of the first cover plate respectively. A driving structure is arranged on the bottom plate, and the driving structure includes a vertical plate, a connecting plate and a cylinder. The two second stirring rods and the first stirring rod are rotatably connected to the first cover plate respectively through bearings. The two second stirring rods are movably arranged in the bottom plate respectively, and the upper ends of the two second stirring rods and the first stirring rod are fixedly installed with gears, and the three gears are meshed with each other.

[0006] As a preferred technical solution of the present invention, the second cover plate is movably installed on the upper end of the containing tube, a circular hole is opened in the middle of the surface of the second cover plate, an annular groove is opened on the side wall of the circular hole, the first stirring rod movably passes through the circular hole, a limiting ring is fixedly installed on the first stirring rod, and the limiting ring is rotatably installed in the annular groove.

[0007] As a preferred technical solution of the present invention, baffles are fixedly connected to both sides of the upper end of the base plate, sliding grooves are provided on the surfaces of the two baffles, and the cylinder is fixedly mounted on the upper end of the base plate.

[0008] As a preferred technical solution of the present invention, a rectangular groove is opened on the surface of the vertical plate, a second motor is fixedly installed on the upper end of the vertical plate, a threaded rod is rotatably installed in the rectangular groove, and an output shaft 2 of the second motor is fixedly connected to the upper end of the threaded rod.

[0009] As a preferred technical solution of the present invention, the connecting plate is fixedly installed on the upper end of the first cover plate, the connecting plate is movably inserted in the rectangular groove, the threaded rod passes through the connecting plate and is threadedly connected to the connecting plate, the end of the rectangular groove is fixedly connected to a limiting rod, and the limiting rod movably passes through the connecting plate.

[0010] As a preferred technical solution of the present invention, the vertical plate is movably mounted on the bottom plate and is located between the two baffles. Both side ends of the vertical plate are fixedly connected with connecting blocks. The two connecting blocks are respectively slidably arranged in the slide grooves. The telescopic rod of the cylinder is fixedly connected to the ends of the vertical plate.

[0011] As a preferred technical solution of the present invention, both side ends of the containing tube are fixedly connected with connecting rods, both sides of the inner wall of the cylinder are fixedly connected with limiting blocks, the two limiting blocks are centrally symmetrically distributed about the midpoint of the cylinder, limiting grooves are provided on the surfaces of the two limiting blocks, and the two connecting rods are movably inserted in the limiting grooves respectively.

[0012] A method for using a processing device for extracting genomes by boiling method comprises the following steps:

[0013] S1: placing the sample in a holding tube, placing the holding tube in a centrifuge, starting the centrifuge, and centrifuging the liquid in the holding tube;

[0014] S2: Take away the upper clear liquid and add a certain amount of SDS into the holding tube:

[0015] S3: The sample and SDS in the holding tube are stirred and mixed, the water in the cylinder is heated to boil, and the holding tube is heated in a boiling water bath;

[0016] S4: Take out the holding tube and cool it down. After it is completely cooled down, centrifuge the liquid in the holding tube and let it settle, and take the supernatant as a PCR reaction template.

[0017] Compared with the prior art, the present invention can achieve the following beneficial effects:

[0018] Firstly, the rotation of the first stirring rod will drive the gear in the middle to rotate. Since the three gears are meshed with each other, the rotation of the first stirring rod will also drive the two second stirring rods to rotate. The rotation of the two second stirring rods will drive the second connecting ring to rotate. The rotation of the second connecting ring will stir and mix the water in the cylinder, which speeds up the heating of the water and shortens the time required for heating, thereby speeding up the heating of the liquid in the holding tube. The rotation of the first stirring rod will stir and mix the sample and SDS, so that they are fully mixed, and further speed up the heating of the liquid in the holding tube, thereby improving the protein variation and DNA extraction effect in the boiling state, thereby improving the practicality of the processing device.

[0019] Secondly, by starting motor 2, the threaded rod rotates, so that the connecting plate moves upward, and the movement of the connecting plate drives the first cover plate to move upward, and the upward movement of the first cover plate drives the first stirring rod to separate from the containing tube, so that the second stirring rod separates from the cylinder body, and then the cylinder is started to move the telescopic rod, and the movement of the telescopic rod drives the vertical plate to move, and the movement of the vertical plate drives the first cover plate, the first stirring rod and the second stirring rod to move. At this time, the containing tube will not be blocked, so that the user can take out the containing tube, which is convenient for the subsequent cooling and subsequent treatment of the liquid in the containing tube, and the operation is more convenient and the use is more convenient.

[0020] Thirdly, the bottom end of the second cover plate is movably plugged into the containing tube to prevent the liquid in the containing tube from splashing out during stirring, and the bottom end of the first cover plate is movably plugged into the cylinder to prevent the water in the cylinder from splashing out during stirring, and effectively prevent the water in the cylinder from splashing into the containing tube, thereby avoiding contamination of the liquid in the containing tube and ensuring the accuracy of protein variation and DNA extraction in the liquid.

[0021] Fourthly, connecting rods are fixedly connected to both side ends of the containing tube, and the two connecting rods are movably inserted into the limiting grooves respectively, which limit the containing tube and prevent the containing tube from tipping over or leaking, making the containing tube more stable when placed in the cylinder. The limiting rods movably penetrate the connecting plate, which limits the connecting plate, making the movement of the connecting plate more stable, thereby making the movement of the first cover plate more stable, thereby ensuring the stable operation of the driving structure. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a structural schematic diagram of the processing device for extracting genome of the present invention;

[0023] Figure 2 It is a structural schematic diagram of the baffle of the present invention;

[0024] Figure 3 It is a structural schematic diagram of the connecting plate of the present invention;

[0025] Figure 4 It is a structural schematic diagram of the cylinder of the present invention;

[0026] Figure 5 It is a structural schematic diagram of the limit block of the present invention;

[0027] Figure 6 is a schematic structural diagram of the first cover plate of the present invention;

[0028] Figure 7 It is a schematic structural diagram of the second cover plate of the present invention.

[0029] Among them: 1. bottom plate; 11. centrifuge; 12. baffle; 13. slide; 2. cylinder; 21. heating rod; 22. limit block; 23. limit groove; 24. first cover plate; 25. bearing; 3. holding tube; 31. connecting rod; 4. motor one; 41. first stirring rod; 42. first connecting ring; 43. first stirring blade; 44. gear; 45. limit ring; 5. second stirring rod; 51. second connecting ring; 52. second stirring blade; 6. second cover plate; 61. circular hole; 62. annular groove; 7. vertical plate; 71. rectangular groove; 72. limit rod; 73. connecting block; 74. motor two; 75. threaded rod; 8. connecting plate; 9. cylinder. DETAILED DESCRIPTION

[0030] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific embodiments, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the embodiments in the implementation mode, other embodiments obtained by those skilled in the art without creative work all belong to the protection scope of the present invention. The experimental methods in the following embodiments, unless otherwise specified, are conventional methods, and the materials, reagents, etc. used in the following embodiments, unless otherwise specified, can all be obtained from commercial channels.

[0031] like Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 and Figure 7As shown, a processing device for extracting genome by boiling method and a method of using the same, comprising a bottom plate 1 and a cylinder 2, wherein a centrifuge 11 is arranged on the bottom plate 1, the cylinder 2 is fixedly mounted on the bottom plate 1, a containing tube 3 is movably mounted in the cylinder 2, a heating rod 21 is arranged in the cylinder 2, a first cover plate 24 is movably mounted on the upper end of the cylinder 2, three bearings 25 are fixedly mounted on the first cover plate 24, a stirring structure is arranged in the cylinder 2, the stirring structure comprises a motor 4, a second stirring rod 5 and a second cover plate 6, the motor 4 is fixedly mounted on the upper end of the first cover plate 24, the output shaft 1 of the motor 4 is fixedly connected to a first stirring rod 41, three first connecting rings 42 are evenly and evenly fixedly mounted on the first stirring rod 41, and each first connecting ring 42 is evenly and evenly fixedly mounted with a connecting rod 42. A first stirring blade 43 is connected, a first connecting ring 42 movably passes through the middle of the first cover plate 24, the number of the second stirring rods 5 is two, the two second stirring rods 5 are symmetrically distributed about the midpoint of the first stirring rod 41, the first stirring rod 41 is movably arranged in the containing tube 3, three second connecting rings 51 are evenly fixedly installed on the two second stirring rods 5, each second connecting ring 51 is fixedly connected with a second stirring blade 52, the two second stirring rods 5 are respectively movably passed through the two side ends of the first cover plate 24, a driving structure is arranged on the bottom plate 1, the driving structure includes a vertical plate 7, a connecting plate 8 and a cylinder 9, the two second stirring rods 5 and the first stirring rod 41 are respectively rotatably connected to the first cover plate 24 through bearings 25, and the two second stirring rods 5 are respectively movably It is arranged in the bottom plate 1, and the upper ends of the two second stirring rods 5 and the first stirring rod 41 are fixedly installed with gears 44, and the three gears 44 are meshed with each other. The second cover plate 6 is movably installed on the upper end of the containing tube 3, and a circular hole 61 is opened in the middle of the surface of the second cover plate 6, and an annular groove 62 is opened on the side wall of the circular hole 61. The first stirring rod 41 movably passes through the circular hole 61, and a limiting ring 45 is fixedly installed on the first stirring rod 41, and the limiting ring 45 is rotatably installed in the annular groove 62. Both sides of the upper end of the bottom plate 1 are fixedly connected with baffles 12, and the surfaces of the two baffles 12 are provided with slide grooves 13. The cylinder 9 is fixedly installed on the upper end of the bottom plate 1, and a rectangular groove 71 is opened on the surface of the vertical plate 7. The upper end of the vertical plate 7 is fixedly installed with a motor 27 4. A threaded rod 75 is rotatably installed in the rectangular groove 71. The output shaft 2 of the motor 2 74 is fixedly connected to the upper end of the threaded rod 75. The connecting plate 8 is fixedly installed on the upper end of the first cover plate 24. The connecting plate 8 is movably inserted in the rectangular groove 71. The threaded rod 75 penetrates the connecting plate 8 and is threadedly connected to the connecting plate 8. The end of the rectangular groove 71 is fixedly connected to the limit rod 72. The sample is placed in the holding tube 3, and the holding tube 3 is placed in the centrifuge 11. The centrifuge 11 is started to centrifuge the liquid in the holding tube 3. After the centrifugation is completed, the upper clear liquid is taken away, and a certain amount of SDS is added to the holding tube 3. Then water is injected into the cylinder 2. The motor 2 74 is started to rotate the threaded rod 75, so that the connecting plate 8 moves downward.The downward movement of the connecting plate 8 will drive the first cover plate 24 to move downward, and the downward movement of the first cover plate 24 will drive the first stirring rod 41, the two second stirring rods 5 and the second cover plate 6 to move downward, so that the first stirring rod 41 is inserted into the containing tube 3, the second stirring rod 5 is inserted into the cylinder 2, and the bottom end of the second cover plate 6 is inserted into the containing tube 3, the cylinder 2 is connected to the power supply, and the water in the cylinder 2 is heated and boiled by the heating rod 21 provided in the cylinder 2, so that the containing tube 3 is heated in a boiling water bath, and the first stirring rod 41 is rotated by starting the motor 4, and the rotation of the first stirring rod 41 drives the first connecting ring 42 to rotate, and the rotation of the first connecting ring 42 drives the first stirring blade 43 to rotate. The sample and SDS in the holding tube 3 are stirred and mixed. After the heating is completed, the holding tube 3 is taken out and then cooled. After the temperature is completely cooled, the holding tube 3 is placed in the centrifuge 11, and the centrifuge 11 is started. The sample and SDS are subjected to centrifugal treatment and are allowed to settle. The supernatant is taken as a PCR reaction template. The rotation of the first stirring rod 41 drives the gear 44 in the middle to rotate. Since the three gears 44 are meshed with each other, the rotation of the first stirring rod 41 drives the two second stirring rods 5 to rotate at the same time. The rotation of the two second stirring rods 5 drives the second connecting ring 51 to rotate. The rotation of the second connecting ring 51 stirs and mixes the water in the cylinder 2, thereby accelerating the heating speed of the water and shortening the time required for heating. The time is increased, thereby accelerating the heating of the liquid in the holding tube 3. The rotation of the first stirring rod 41 stirs and mixes the sample and SDS, so that they are fully mixed, and further accelerates the heating of the liquid in the holding tube 3, thereby improving the protein variation and DNA extraction effect in the boiling state of the liquid, and improving the practicality of the processing device. By starting the second motor 74, the threaded rod 75 rotates, so that the connecting plate 8 moves upward, and the movement of the connecting plate 8 drives the first cover plate 24 to move upward. The upward movement of the first cover plate 24 drives the first stirring rod 41 to separate from the holding tube 3, so that the second stirring rod 5 separates from the cylinder 2, and then the cylinder 9 is started to move the telescopic rod, and the movement of the telescopic rod drives the vertical plate 7 to move upward. The movement of the vertical plate 7 will drive the first cover plate 24, the first stirring rod 41 and the second stirring rod 5 to move. At this time, the holding tube 3 will not be blocked, so that the user can take out the holding tube 3, which is convenient for the subsequent cooling and subsequent treatment of the liquid in the holding tube 3. The operation is more convenient and the use is more convenient. The bottom end of the second cover plate 6 is movably plugged into the holding tube 3 to prevent the liquid in the holding tube 3 from splashing out during the stirring process. The bottom end of the first cover plate 24 is movably plugged into the cylinder 2 to prevent the water in the cylinder 2 from splashing out during the stirring process, and effectively prevent the water in the cylinder 2 from splashing into the holding tube 3, thereby avoiding contamination of the liquid in the holding tube 3, ensuring the accuracy of protein variation and DNA extraction in the liquid.The two end portions of the holding tube 3 are fixedly connected with connecting rods 31, and the two connecting rods 31 are respectively movably inserted into the limiting grooves 23, which play a limiting role on the holding tube 3, and prevent the holding tube 3 from tipping over or leaking, so that the holding tube 3 is placed in the cylinder 2 more stably. The limiting rod 72 movably penetrates the connecting plate 8, which plays a limiting role on the connecting plate 8, so that the movement of the connecting plate 8 is more stable, so that the movement of the first cover plate 24 is more stable, and the stable operation of the driving structure is ensured. The limiting rod 72 movably penetrates the connecting plate 8, and the vertical plate 7 movably penetrates the connecting plate 8. It is installed on the bottom plate 1 and between the two baffles 12. The two ends of the vertical plate 7 are fixedly connected with connecting blocks 73. The two connecting blocks 73 are slidably set in the slide grooves 13. The telescopic rod of the cylinder 9 is fixedly connected to the ends of the vertical plate 7. The two ends of the holding tube 3 are fixedly connected with connecting rods 31. The two sides of the inner wall of the cylinder 2 are fixedly connected with limited blocks 22. The two limited blocks 22 are symmetrically distributed about the midpoint of the cylinder 2. The surfaces of the two limited blocks 22 are provided with limited grooves 23. The two connecting rods 31 are movably inserted in the limited grooves 23.

[0032] When in use, the sample is placed in the holding tube 3, the holding tube 3 is placed in the centrifuge 11, the centrifuge 11 is started, and the liquid in the holding tube 3 is centrifuged. After the centrifugation is completed, the upper clear liquid is taken away, and a certain amount of SDS is added to the holding tube 3, and then water is injected into the cylinder 2, and the motor 2 74 is started to rotate the threaded rod 75, so that the connecting plate 8 moves downward, and the downward movement of the connecting plate 8 drives the first cover plate 24 to move downward, and the downward movement of the first cover plate 24 drives the first stirring rod 41, the two second stirring rods 5 and the second cover plate 6 to move downward, so that the first stirring rod 41 is inserted into the holding tube 3, the second stirring rod 5 is inserted into the cylinder 2, and the bottom end of the second cover plate 6 is inserted into the holding tube 3. The cylinder 2 is connected to a power source, and the water in the cylinder 2 is heated and boiled by a heating rod 21 provided in the cylinder 2, so that the holding tube 3 is heated in a boiling water bath, and the first stirring rod 41 is rotated by starting the motor 4, and the first connecting ring 42 is driven to rotate by the rotation of the first stirring rod 41, and the first connecting ring 42 is driven to rotate by the rotation of the first stirring blade 43, so that the sample and SDS in the holding tube 3 are stirred and mixed. After the heating is completed, the holding tube 3 is taken out, and then the holding tube 3 is cooled. After the temperature is completely cooled, the holding tube 3 is placed in a centrifuge 11, and the centrifuge 11 is started, and the sample is centrifuged and allowed to settle, and the supernatant is taken as a PCR reaction template;

[0033] The rotation of the first stirring rod 41 will drive the gear 44 in the middle to rotate. Since the three gears 44 are meshed with each other, the rotation of the first stirring rod 41 will drive the two second stirring rods 5 to rotate. The rotation of the two second stirring rods 5 will drive the second connecting ring 51 to rotate. The rotation of the second connecting ring 51 will stir and mix the water in the cylinder 2, thereby accelerating the speed of heating the water and shortening the time required for heating, thereby accelerating the heating of the liquid in the holding tube 3. The rotation of the first stirring rod 41 will stir and mix the sample and SDS, so that they are fully mixed, and further accelerate the heating of the liquid in the holding tube 3, thereby improving the protein variation and DNA extraction effect in the boiling state of the liquid, thereby improving the practicality of the processing device.

[0034] By starting the second motor 74, the threaded rod 75 rotates, so that the connecting plate 8 moves upward, and the movement of the connecting plate 8 drives the first cover plate 24 to move upward, and the first cover plate 24 moves upward to drive the first stirring rod 41 to separate from the containing tube 3, so that the second stirring rod 5 separates from the cylinder 2, and then the cylinder 9 is started to move the telescopic rod, and the movement of the telescopic rod drives the vertical plate 7 to move, and the movement of the vertical plate 7 drives the first cover plate 24, the first stirring rod 41 and the second stirring rod 5 to move, and the containing tube 3 will not be blocked at this time, so that the user can take out the containing tube 3, and the subsequent cooling and subsequent treatment of the liquid in the containing tube 3 are convenient, and the operation is more convenient and the use is more convenient;

[0035] The bottom end of the second cover plate 6 is movably inserted in the holding tube 3 to prevent the liquid in the holding tube 3 from splashing out during the stirring process. The bottom end of the first cover plate 24 is movably inserted in the cylinder 2 to prevent the water in the cylinder 2 from splashing out during the stirring process, and effectively prevent the water in the cylinder 2 from splashing into the holding tube 3, thereby avoiding contamination of the liquid in the holding tube 3, and ensuring the accuracy of protein variation and DNA extraction in the liquid.

[0036] Both side ends of the containing tube 3 are fixedly connected with connecting rods 31, and the two connecting rods 31 are movably inserted into the limiting grooves 23 respectively, which limit the containing tube 3 and prevent the containing tube 3 from tipping over or leaking, so that the containing tube 3 is placed in the cylinder 2 more stably. The limiting rod 72 movably penetrates the connecting plate 8, which limits the connecting plate 8, so that the movement of the connecting plate 8 is more stable, thereby making the movement of the first cover plate 24 more stable, thereby ensuring the stable operation of the driving structure.

[0037] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited thereto, and various changes can be made within the knowledge scope of technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. A processing device for extracting genome by boiling method, comprising a bottom plate (1) and a cylinder (2), characterized in that: The bottom plate (1) is provided with a centrifuge (11); the cylinder (2) is fixedly mounted on the bottom plate (1); a containing tube (3) is movably mounted in the cylinder (2); a heating rod (21) is arranged in the cylinder (2); a first cover plate (24) is movably mounted on the upper end of the cylinder (2); three bearings (25) are fixedly mounted on the first cover plate (24); a stirring structure is arranged in the cylinder (2); the stirring structure comprises a motor (4), a second stirring rod (5) and a second cover plate (6); the motor (4) is fixedly mounted on the upper end of the first cover plate (24); an output shaft (1) of the motor (4) is fixedly connected to a first stirring rod (41); three first connecting rings (42) are fixedly mounted on the first stirring rod (41) at equal distances; each of the first connecting rings (42) is provided with a first connecting ring (42); The connecting ring (42) is evenly and fixedly connected with first stirring blades (43), the first connecting ring (42) movably passes through the middle of the first cover plate (24), the number of the second stirring rods (5) is two, the two second stirring rods (5) are symmetrically distributed about the midpoint of the first stirring rod (41), the first stirring rod (41) is movably arranged in the containing tube (3), three second connecting rings (51) are evenly and fixedly installed on the two second stirring rods (5), each of the second connecting rings (51) is fixedly connected with a second stirring blade (52), the two second stirring rods (5) respectively movably pass through the two side ends of the first cover plate (24), and the bottom plate (1) is provided with a driving structure, the driving structure comprising a vertical plate (7), a connecting plate (8) and a cylinder (9); The two second stirring rods (5) and the first stirring rod (41) are rotatably connected to the first cover plate (24) via bearings (25), and the two second stirring rods (5) are movably arranged in the bottom plate (1); Gears (44) are fixedly mounted on the upper ends of the two second stirring rods (5) and the first stirring rod (41), and the three gears (44) are meshed with each other; The second cover plate (6) is movably mounted on the upper end of the containing tube (3); a circular hole (61) is provided in the middle of the surface of the second cover plate (6); a ring groove (62) is provided on the side wall of the circular hole (61); the first stirring rod (41) movably passes through the circular hole (61); a limiting ring (45) is fixedly mounted on the first stirring rod (41); the limiting ring (45) is rotatably mounted in the ring groove (62); Baffles (12) are fixedly connected to both sides of the upper end of the bottom plate (1), and sliding grooves (13) are provided on the surfaces of the two baffles (12). The cylinder (9) is fixedly mounted on the upper end of the bottom plate (1); A rectangular groove (71) is formed on the surface of the vertical plate (7), a second motor (74) is fixedly mounted on the upper end of the vertical plate (7), a threaded rod (75) is rotatably mounted in the rectangular groove (71), and an output shaft (2) of the second motor (74) is fixedly connected to the upper end of the threaded rod (75); The connecting plate (8) is fixedly mounted on the upper end of the first cover plate (24); the connecting plate (8) is movably inserted into the rectangular groove (71); the threaded rod (75) passes through the connecting plate (8) and is threadedly connected to the connecting plate (8); the end of the rectangular groove (71) is fixedly connected to a limiting rod (72); the limiting rod (72) movably passes through the connecting plate (8).

2. The processing device for extracting genome by boiling method according to claim 1, characterized in that: The vertical plate (7) is movably mounted on the bottom plate (1) and is located between the two baffles (12). Both end portions of the vertical plate (7) are fixedly connected with connecting blocks (73). The two connecting blocks (73) are respectively slidably arranged in the slide grooves (13). The telescopic rod of the cylinder (9) is fixedly connected to the ends of the vertical plate (7).

3. The processing device for extracting genome by boiling method according to claim 2, characterized in that: Both end portions of the containing tube (3) are fixedly connected with connecting rods (31), and both sides of the inner wall of the cylinder (2) are fixedly connected with limiting blocks (22), the two limiting blocks (22) are centrally symmetrically distributed about the midpoint of the cylinder (2), and limiting grooves (23) are provided on the surfaces of the two limiting blocks (22), and the two connecting rods (31) are movably inserted in the limiting grooves (23) respectively.

4. A method for using the processing device for extracting genome by boiling method as claimed in any one of claims 1 to 3, characterized in that: The following steps are involved: S1: placing a sample in a holding tube (3), placing the holding tube (3) in a centrifuge (11), starting the centrifuge (11), and centrifuging the liquid in the holding tube (3); S2: Take away the upper clear liquid and add a certain amount of SDS into the holding tube (3): S3: The sample and SDS in the holding tube (3) are stirred and mixed, the water in the cylinder (2) is heated to boil, and the holding tube (3) is heated in a boiling water bath; S4: taking out the holding tube (3), cooling the holding tube (3), and after the temperature has completely cooled down, centrifuging the liquid in the holding tube (3) and allowing it to settle, and taking the supernatant as a PCR reaction template.

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