A cell washing method and device

Abstract

The present invention relates to a cell elutriation method and device, which is used to replace the stock solution surrounding cells in the cell fluid with a cleaning solution. The cell elutriation method comprises: providing a cleaning solution and a stock solution; delivering the cleaning solution and the stock solution to a centrifugal system in a dynamic ratio, and performing an elutriation treatment on the cell fluid in the centrifugal system to obtain a final product. The cell elutriation method uses a mixed liquid of a cleaning solution and a stock solution, and controls the ratio and action time thereof, so that the liquid composition around the cells changes slowly, eliminates concentration mutations, and reduces cell damage.

Description

Technical Field

[0001] The present invention relates to the technical field of cell processing, and in particular to a cell washing method and device. Background Art

[0002] In the field of cell processing, cells often need to be washed. For example, after cell culture, the culture medium must be washed away by washing and replaced with a liquid environment with buffer as the main component; for example, after cell separation, the serum and separation fluid must be washed away by washing and replaced with a liquid environment with culture medium as the main component. The usual method of washing is to complete the sedimentation of the cells in a centrifugal structure, discharge the supernatant, and then add the cleaning fluid, and repeat this process. The washing process may include resuspension. The residual rate is defined as: the ratio of the concentration of a solute after washing to the concentration of a solute before washing. During the cell washing process, when the residual rate reaches a pre-defined range, the washing is completed. The liquid environment around the cells has changed after washing, which is also the purpose of washing.

[0003] When cells are placed in a fluid, due to the fluid's non-shear resistance, when not static, the cells are in a shear force environment, which damages the cell surface and increases osmotic fragility. Especially during the centrifugation process, the cells are subjected to more severe shear damage and are very sensitive to changes in osmotic pressure. When the cleaning fluid is added, the cells suddenly change from one liquid environment to another. The change in osmotic pressure caused by the change in composition will quickly damage the cells or even rupture them. It is also a consensus that changes in osmotic pressure can damage cells, so albumin is widely used in the industry to maintain cell osmotic pressure, but the effect is not ideal. Summary of the invention

[0004] The present invention provides a cell washing method and device, which uses a mixed liquid of cleaning liquid and stock solution and controls the ratio and action time thereof, so as to slowly change the liquid composition around the cells, eliminate concentration mutations and reduce cell damage.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] The present invention provides a cell washing method, which comprises the following steps:

[0007] Provide cleaning solution and stock solution;

[0008] The cleaning solution and the stock solution are transported to a centrifugal system in a dynamic ratio, and the cell solution in the centrifugal system is subjected to elutriation treatment to obtain a final product.

[0009] Furthermore, providing the cleaning fluid comprises:

[0010] The cleaning liquid is placed in a cleaning liquid container, and the cleaning liquid container is connected to the centrifugal system through a cleaning pipeline; a first flow control device for controlling the cleaning liquid delivery flow is installed in the cleaning pipeline.

[0011] Furthermore, providing the stock solution comprises:

[0012] extracting the supernatant, and collecting the supernatant as the stock solution in a stock solution container;

[0013] The raw liquid container is connected to the centrifugal system through a raw liquid pipeline; a second flow control device for controlling the raw liquid delivery flow is installed in the raw liquid pipeline.

[0014] Furthermore, the extraction supernatant comprises:

[0015] The cell sample is centrifuged by the centrifugal system, and the supernatant is extracted during the centrifugation process; the cell sample contains the cell fluid and the supernatant.

[0016] Furthermore, the step of delivering the cleaning solution and the stock solution to the centrifugal system in a dynamic ratio comprises:

[0017] Sending the dynamic ratio to the first flow control device and the second flow control device; the dynamic ratio includes the percentage of cleaning liquid and the percentage of raw liquid;

[0018] adjusting the flow rate of the cleaning liquid according to the received percentage of the cleaning liquid by the first flow control device, and adjusting the flow rate of the raw liquid according to the received percentage of the raw liquid by the second flow control device;

[0019] The adjusted flow rate of the cleaning solution and the flow rate of the original solution are combined and transmitted to the centrifugal system.

[0020] Furthermore, the percentage of the cleaning solution and the percentage of the stock solution both vary in a gradient.

[0021] Furthermore, the adjusting the flow rate of the cleaning liquid according to the received percentage of the cleaning liquid by the first flow control device, and adjusting the flow rate of the stock liquid according to the received percentage of the stock liquid by the second flow control device, include:

[0022] determining a first rotational speed of a pump in the first flow control device according to the received percentage of the cleaning liquid, and adjusting the flow rate of the cleaning liquid by the first rotational speed;

[0023] According to the received percentage of the raw liquid, a second rotational speed of the pump in the second flow control device is determined, and the flow rate of the raw liquid is adjusted according to the second rotational speed.

[0024] Furthermore, the adjusting the flow rate of the cleaning liquid according to the received percentage of the cleaning liquid by the first flow control device, and adjusting the flow rate of the stock liquid according to the received percentage of the stock liquid by the second flow control device, further includes:

[0025] Determining a first flow resistance of a flow-limiting structure in the first flow control device according to the received percentage of the cleaning liquid, and adjusting the flow rate of the cleaning liquid by the first flow resistance;

[0026] According to the received percentage of the raw liquid, a second flow resistance of the flow limiting structure in the second flow control device is determined, and the flow rate of the raw liquid is adjusted by the second flow resistance.

[0027] In addition, the present invention also provides a cell washing device for the above-mentioned cell washing method, the cell washing device comprising a centrifugal system, a cleaning liquid container, a cleaning pipeline, a stock liquid container, a stock liquid pipeline, a first flow control device and a second flow control device;

[0028] The centrifugal system includes a centrifugal container for centrifugation and elutriation;

[0029] The cleaning liquid container is connected to the centrifugal container through the cleaning pipeline, and a first flow control device for controlling the flow rate of the cleaning liquid is installed in the cleaning pipeline;

[0030] The raw liquid container is connected to the centrifugal container through the raw liquid pipeline, and a second flow control device for controlling the raw liquid delivery flow rate is installed in the raw liquid pipeline.

[0031] Furthermore, it also includes a liquid inlet main pipe;

[0032] One end of the liquid inlet main pipe is connected to the centrifugal container, and the other end is connected to both the raw liquid pipeline and the cleaning pipeline.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] In the cell washing method of the present invention, during the cell washing process, the stock solution and the cleaning solution are transported to the centrifugal system in a dynamic ratio, so that the cell solution in the centrifugal system is washed and the final product is obtained. The liquid environment around the cells can be slowly changed by adjusting the dynamic ratio, and the liquid environment can be slowly transitioned from pure stock solution to pure cleaning solution, so that the cells gradually adapt to the external environment and reduce cell loss. Therefore, the above-mentioned cell washing method uses a mixed liquid of cleaning solution-stock solution to slowly change the liquid composition around the cells, eliminate concentration mutations, and reduce cell damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 is a flow chart of the cell panning method of the present invention;

[0036] Figure 2 Two washing solution percentage schemes used in the cell panning process;

[0037] Figure 3 To adopt Figure 2 Comparison of the viable cell yields obtained with the two washing solution percentage schemes;

[0038] Figure 4 A schematic diagram of the principle structure of the cell washing device of the present invention;

[0039] Figure 5 Another schematic diagram of the principle structure of the cell washing device of the present invention.

[0040] Among them, 1-centrifuge container, 2-cleaning liquid container, 3-cleaning pipeline, 4-first flow control device, 5-raw liquid container, 6-raw liquid pipeline, 7-second flow control device, 8-liquid inlet main pipe, 9-pump DETAILED DESCRIPTION

[0041] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0042] In the embodiment of the present invention, the "elutriation liquid" is a liquid used to elutriate cells, and the "cleaning liquid" is a reagent selected before treating cells. The purpose of elutriation is to replace the liquid around the cells with the cleaning liquid. The "stock solution" is the liquid component in which the cells are located before the cells are elutriated, that is, the liquid component that does not contain cells in the cell solution to be treated, which can be obtained by centrifuging the cells to settle and then extracting the supernatant. In the embodiment of the present invention, a mixture of the cleaning liquid and the stock solution is used as the elutriation liquid.

[0043] The present invention proposes a "gradient concentration washing" method, in which the supernatant is collected during centrifugation to obtain the stock solution, and the stock solution and the washing solution are injected into the cell container in proportion, so that the liquid environment around the cells changes slowly, and the transition from the pure stock solution environment to the pure washing solution environment is slowly made, so that the cells gradually adapt to the environment and cell damage is reduced.

[0044] Embodiment 1

[0045] This embodiment provides a cell washing method, such as Figure 1As shown, the method is used to replace the original liquid around the cells in the cell liquid with a cleaning liquid. The cell washing method includes the following steps:

[0046] Step S10, providing a cleaning solution and a stock solution;

[0047] Step S20, transporting the cleaning solution and the stock solution to the centrifugal system in a dynamic ratio, performing elutriation treatment on the cell solution in the centrifugal system to obtain a final product;

[0048] The washing process can be that the centrifugal container 1 in the centrifugal system receives the mixed liquid of the cleaning liquid and the original liquid in a dynamic proportion on one side, and extracts the mixed liquid from the centrifugal container 1 on the other side, forming an intermittent flow or continuous flow replacement process, for example: the upper part of the centrifugal container 1 is input, and the lower part is extracted and discharged to the waste liquid bag, so that the fluid can flow continuously. It can also be intermittent input and then intermittent extraction from the centrifugal container, which can be asynchronous or synchronous.

[0049] The centrifugal system is a system that performs rotary centrifugation on the centrifugal container 1 to separate the cell solution and the mixed liquid of the cleaning solution-stock solution into layers, and can perform elutriation treatment in the layered state.

[0050] When the above-mentioned cell washing method is used for cell washing, the stock solution and the cleaning solution are transported to the centrifugal system in a dynamic ratio, so that the cell fluid in the centrifugal system is washed and the final product is obtained. The stock solution and the cleaning solution can be simultaneously transported to the centrifugal system according to the stock solution percentage and the cleaning solution percentage in the dynamic ratio by adjusting the dynamic ratio. The cleaning solution percentage of the cleaning solution can be gradually increased from 0% to 100%, and the stock solution percentage of the stock solution can be gradually reduced from 100% to 0%, so that the stock solution around the cells is gradually reduced, so as to achieve a slow change in the liquid environment around the cells, and slowly transition from pure stock solution to pure cleaning solution, so that the cells gradually adapt to the external environment and reduce cell loss. Therefore, the above-mentioned cell washing method uses a mixed liquid of cleaning solution-stock solution to slowly change the liquid composition around the cells, eliminate concentration mutations, and reduce cell damage.

[0051] In a specific embodiment, providing the cleaning solution and the stock solution in step S10 can be achieved by the following steps:

[0052] The cleaning liquid is placed in a cleaning liquid container 2, and the cleaning liquid container 2 is connected to the centrifugal system through a cleaning pipeline 3; a first flow control device 4 for controlling the flow rate of the cleaning liquid is installed in the cleaning pipeline 3;

[0053] The supernatant is extracted and collected as the stock solution in the stock solution container 5; when the supernatant is extracted, the cell sample is centrifuged by the centrifugal system and the supernatant is obtained during the centrifugation process; the cell sample contains cell fluid and supernatant; the stock solution container 5 is connected to the centrifugal system through the stock solution pipeline 6; a second flow control device 7 for controlling the stock solution delivery flow rate is installed in the stock solution pipeline 6.

[0054] In step S20, the cleaning liquid and the stock liquid are transported to the centrifugal system in a dynamic ratio, which may include the following steps:

[0055] The dynamic ratio is sent to the first flow control device 4 and the second flow control device 7; the dynamic ratio includes the percentage of cleaning liquid and the percentage of original liquid; the dynamic ratio can change with time and change dynamically, which can be understood as the changes of the percentage of cleaning liquid and the percentage of original liquid can be set according to needs, for example: the percentage of cleaning liquid and the percentage of original liquid can change in a sinusoidal fluctuation manner, or in a linear increasing / linear decreasing manner, or in a sawtooth increasing or sawtooth decreasing manner, etc.

[0056] Among them, the percentage of the cleaning solution and the percentage of the stock solution can both change in a gradient, that is, the ratio is adjusted in a gradient at each predetermined time interval, such as: at each predetermined time interval, the gradient concentration of the cleaning solution gradually increases, and the proportion of the cleaning solution increases by 25%, which can be 0, 25%, 50%, 75%, 100% in sequence, while the gradient concentration of the stock solution gradually decreases, and the proportion of the stock solution decreases by 25%, which can be 100%, 75%, 50%, 25%, 0 in sequence. The adjustment ratio of the cleaning solution can also be 15%, 20%, 30%, etc. The improvement of cell yield and status can be confirmed by experiments, such as Figure 2 and Figure 3 As shown, the cultured T cells in the same bag were divided into two bags. The experimental group used the gradient washing method to wash the cells, and the control group used the washing solution directly to wash. The washing solution composition was as follows Figure 2 As shown, the cell yield and viability are Figure 3 As shown, through Figure 3 It can be seen that the advantages of gradient cleaning are very obvious.

[0057] In one embodiment, the sum of the cleaning solution percentage and the stock solution percentage is one.

[0058] In another embodiment, the sum of the cleaning solution percentage and the original solution percentage is less than one, that is, the dynamic ratio also includes other component percentages, for example: the other component percentage is the percentage of air entering after sterile filtration in a sterile environment.

[0059] The flow rate of the cleaning liquid is adjusted by the first flow control device 4 according to the percentage of the cleaning liquid received, and the flow rate of the raw liquid is adjusted by the second flow control device 7 according to the percentage of the raw liquid received;

[0060] The adjusted flow of the cleaning solution and the flow of the original solution are combined and transmitted to the centrifugal system.

[0061] When the first flow control device 4 adjusts the flow rate of the cleaning liquid according to the percentage of the cleaning liquid received and the second flow control device 7 adjusts the flow rate of the stock liquid according to the percentage of the stock liquid received, the following two implementations can be used:

[0062] Implementation Method 1

[0063] Determine a first rotation speed of the pump in the first flow control device 4 according to the percentage of the cleaning liquid received, and adjust the flow rate of the cleaning liquid by the first rotation speed;

[0064] According to the percentage of the received raw liquid, the second rotation speed of the pump in the second flow control device 7 is determined, and the flow rate of the raw liquid is adjusted by the second rotation speed.

[0065] In this embodiment, the first flow control device 4 and the second flow control device 7 are both pumps. The capacity transmitted per unit time is adjusted by adjusting the rotation speed of the two pumps, and no pump is provided on the path for merging and transmitting to the centrifugal system. The two pumps drive the raw liquid in the raw liquid pipeline 5 and the cleaning liquid in the cleaning pipeline 3 to merge and transmit to the centrifugal system, so that the precise flow rate of the raw liquid is controlled by the pump in the raw liquid pipeline 5 according to the percentage of raw liquid received, and the precise flow rate of the cleaning liquid is controlled by the pump in the cleaning pipeline 3 according to the percentage of cleaning liquid received.

[0066] Implementation Method 2

[0067] Determine the first flow resistance of the flow-limiting structure in the first flow control device 4 according to the percentage of the cleaning liquid received, and adjust the flow rate of the cleaning liquid by the first flow resistance;

[0068] According to the percentage of the received raw liquid, the second flow resistance of the flow-limiting structure in the second flow control device 7 is determined, and the flow rate of the raw liquid is adjusted by the second flow resistance.

[0069] In this embodiment, the first flow control device 4 and the second flow control device 7 are both flow limiting structures, which control the volume flowing through per unit time through the flow limiting structure to adjust the flow rate. A pump 9 for driving the flow of the mixed liquid is provided on the path of the confluence transmission to the centrifugal system. The liquids in the cleaning pipeline 3 and the raw liquid pipeline 6 are driven to flow at the same flow rate by one pump 9, but the flow rates allowed to pass through the cleaning pipeline 3 and the raw liquid pipeline 6 are different to achieve dynamic proportional changes. The flow limiting structure is to change the diameter of the pipeline from the cleaning liquid / raw liquid to the confluence by adjusting the size of the control valve orifice (compressing the pipeline into the required flow aperture to control the flow), for example, making the control valve orifice smaller, and deforming (narrowing) the pipeline passing through the control valve orifice by compression, so that the flow rate becomes less.

[0070] The theoretical basis of the cell elutriation method of the present invention is: when the liquid environment around the cell changes, the concentration difference inside and outside the cell will not be reduced immediately, mainly requiring the joint efforts of channel proteins and another energy-consuming protein-sodium potassium pump to maintain the stability of potassium ion concentration inside and outside the cell. In cell processing, cells are inevitably subjected to fluid shear force, the protein on the membrane is damaged, and the transport capacity decreases. During the elutriation process, if the cleaning solution enters the cell concentrate, the salt concentration and the concentration of protein substances that maintain the osmotic pressure will also change with the instantaneous change, and the cell osmotic pressure is difficult to maintain, which will damage or even break. The above-mentioned cell elutriation method has the function of mixing the cleaning solution with the stock solution and then squeezing into the centrifugal system for cell elutriation, which can artificially control the liquid components around the cell, so that its molecular concentration and colloid concentration slowly change, and give the cell time to adjust the osmotic pressure, which can effectively avoid the cell damage caused by the change of the liquid environment, and improve the cell state and viability.

[0071] Embodiment 2

[0072] This embodiment provides a cell washing device for performing the above-mentioned cell washing method, such as Figure 4 and Figure 5 As shown, the cell washing device includes a centrifugal system, a cleaning liquid container 2, a cleaning pipeline 3, a raw liquid container 5, a raw liquid pipeline 6, a first flow control device 4 and a second flow control device 7; the centrifugal system includes a centrifugal container 1 for centrifugation and washing; the cleaning liquid container 2 is connected to the centrifugal container 1 through the cleaning pipeline 3, and the cleaning pipeline 3 is equipped with a first flow control device 4 for controlling the cleaning liquid delivery flow rate; the raw liquid container 5 is connected to the centrifugal container 1 through the raw liquid pipeline 6, and the raw liquid pipeline 6 is equipped with a second flow control device 7 for controlling the raw liquid delivery flow rate.

[0073] The above-mentioned cell washing device can accurately control the flow of the stock solution and the cleaning solution through the flow control device arranged in the pipeline, thereby adjusting the ratio of the stock solution and the cleaning solution, and then achieving the purpose of controlling the concentration of the stock solution and the cleaning solution, realizing the gradient concentration change of the cleaning solution, making the liquid composition around the cells change slowly, eliminating concentration mutations, and reducing cell damage.

[0074] The cell washing device may further include a liquid inlet manifold 8; one end of the liquid inlet manifold 8 is connected to the centrifugal container 1, and the other end is connected to both the stock liquid pipeline 6 and the cleaning liquid pipeline 3. The stock liquid pipeline 6 and the cleaning liquid pipeline are merged by the liquid inlet manifold 8 before entering the centrifugal container 1, which can improve the mixing efficiency of the stock liquid and the cleaning liquid.

[0075] In the above cell washing device, Figure 4 As shown, the first flow control device 4 and the second flow control device 7 can both be pumps, and the flow rates of the liquids in the corresponding pipelines can be directly and accurately controlled by the pumps. Figure 5 As shown, the first flow control device 4 and the second flow control device 7 both include a flow limiting structure. At the same time, a pump 9 is installed in the liquid inlet main pipe 8 connected to both the raw liquid pipeline 6 and the cleaning pipeline 3. The liquid in the raw liquid pipeline 6 and the cleaning pipeline 3 is provided with a driving force by the same pump 9, while the cleaning pipeline 3 and the raw liquid pipeline 6 use different flow limiting structures to generate flow resistance to the liquid to adjust the flow rate, that is, the first flow control device 4 controls the flow resistance of the liquid in the cleaning pipeline 3 through the flow limiting structure according to the percentage of the received cleaning liquid, and the second flow control device 7 controls the flow resistance of the liquid in the raw liquid pipeline 6 through its flow limiting structure according to the percentage of the received raw liquid, so as to achieve the purpose of regulating the flow rate. The pump can be a peristaltic pump; the flow limiting structure can be a flow control valve such as a solenoid valve. The flow rate of the cleaning liquid can be controlled by the first flow control device 4, and the flow rate of the raw liquid can be controlled by the second flow control device 7, so that the composition of the elutriation liquid can be adjusted. The first flow control device 4 and the second flow control device 7 can be implemented by a simple pump, which provides a power source to the liquid and controls the flow ratio of the two components by adjusting the flow rate of the pump; or the pump can provide a power source to the liquid, and then adjust the flow resistance of the two paths through a flow limiting structure, thereby achieving the effect of adjusting the flow ratio.

[0076] The above-mentioned stock liquid container 5 and cleaning liquid container 2 can be liquid bags. The supernatant can be extracted in a continuous flow state. During the elutriation process, continuous flow elutriation can be performed, repeated elutriation can be performed, or a combination of the two.

[0077] Obviously, those skilled in the art can make various changes and modifications to the embodiments of the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A cell panning method, characterized in that: The cell panning method comprises the following steps: Providing a cleaning liquid and a stock solution; the providing of the cleaning liquid comprises: placing the cleaning liquid in a cleaning liquid container, connecting the cleaning liquid container to the centrifugal system through a cleaning pipeline; a first flow control device for controlling the flow rate of the cleaning liquid is installed in the cleaning pipeline; The providing of the stock solution comprises: extracting the supernatant and collecting the supernatant as the stock solution in a stock solution container; connecting the stock solution container to the centrifugal system through a stock solution pipeline; a second flow control device for controlling the flow rate of the stock solution is installed in the stock solution pipeline; The extracting of the supernatant comprises: centrifuging the cell sample by the centrifugal system and extracting the supernatant during the centrifugation process; the cell sample comprises the cell fluid and the supernatant; The cleaning liquid and the stock liquid are transported to the centrifugal system in a dynamic ratio, and the cell liquid in the centrifugal system is washed to obtain a final product; the cleaning liquid and the stock liquid are transported to the centrifugal system in a dynamic ratio, including: sending the dynamic ratio to the first flow control device and the second flow control device; the dynamic ratio includes the cleaning liquid percentage and the stock liquid percentage; adjusting the flow of the cleaning liquid according to the received cleaning liquid percentage by the first flow control device, and adjusting the flow of the stock liquid according to the received stock liquid percentage by the second flow control device; the adjusted flow of the cleaning liquid and the flow of the stock liquid are combined and transmitted to the centrifugal system.

2. The cell washing method according to claim 1, characterized in that The percentage of the cleaning solution and the percentage of the stock solution both vary in a gradient.

3. The cell washing method according to claim 1, characterized in that The first flow control device adjusts the flow of the cleaning liquid according to the received percentage of the cleaning liquid, and the second flow control device adjusts the flow of the stock liquid according to the received percentage of the stock liquid, including: determining a first rotational speed of a pump in the first flow control device according to the received percentage of the cleaning liquid, and adjusting the flow rate of the cleaning liquid by the first rotational speed; According to the received percentage of the raw liquid, a second rotational speed of the pump in the second flow control device is determined, and the flow rate of the raw liquid is adjusted according to the second rotational speed.

4. The cell washing method according to claim 1, characterized in that The method of adjusting the flow rate of the cleaning liquid according to the received percentage of the cleaning liquid by the first flow control device, and adjusting the flow rate of the raw liquid according to the received percentage of the raw liquid by the second flow control device, further includes: Determining a first flow resistance of a flow-limiting structure in the first flow control device according to the received percentage of the cleaning liquid, and adjusting the flow rate of the cleaning liquid by the first flow resistance; According to the received percentage of the raw liquid, a second flow resistance of the flow limiting structure in the second flow control device is determined, and the flow rate of the raw liquid is adjusted by the second flow resistance.

5. A cell washing device for use in the cell washing method according to any one of claims 1 to 4, characterized in that: It includes a centrifugal system, a cleaning liquid container, a cleaning pipeline, a raw liquid container, a raw liquid pipeline, a first flow control device and a second flow control device; The centrifugal system includes a centrifugal container for centrifugation and elutriation; The cleaning liquid container is connected to the centrifugal container through the cleaning pipeline, and a first flow control device for controlling the flow rate of the cleaning liquid is installed in the cleaning pipeline; The raw liquid container is connected to the centrifugal container through the raw liquid pipeline, and a second flow control device for controlling the raw liquid delivery flow rate is installed in the raw liquid pipeline; The first flow control device and the second flow control device both include a flow limiting structure. The cleaning pipeline and the raw liquid pipeline use different flow limiting structures to generate flow resistance for the liquid to adjust the flow rate. The first flow control device controls the flow resistance of the liquid in the cleaning pipeline through the flow limiting structure under the first flow control device according to the percentage of cleaning liquid received. The second flow control device controls the flow resistance of the liquid in the raw liquid pipeline through the flow limiting structure under the second flow control device according to the percentage of raw liquid received.

6. The cell washing device according to claim 5, characterized in that: Also included is a liquid inlet manifold; One end of the liquid inlet main pipe is connected to the centrifugal container, and the other end is connected to both the raw liquid pipeline and the cleaning pipeline.

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