A special device for fixing large tissue slices of the whole prostate organ and a method for making the same

Abstract

The invention relates to a special device for fixing large tissue slices of a prostate organ and a method for making the same. The special device for fixing large tissue slices of a prostate organ specifically comprises a bottom plate box for placing tissues, a top cover box with a pressure-applying function and a closed box for placing a fixing frame for fixing with a fixing liquid. The special method for making large tissue slices of a prostate organ comprises pre-processing, slice photographing and recording, tissue fixing processing, dehydration, wax dipping, embedding and marking, slicing, conventional slicing, baking and drying, secondary dehydration, wax melting, HE staining, cover slicing, and finally slicing to prepare a complete large tissue slide for a pathologist to make a diagnosis of the spatial distribution of prostate tissues. The invention solves the problem of long fixing time and the like; the sequence is orderly, the fixing time is reduced, and the time cost is saved.

Description

Technical Field

[0001] The invention relates to the field of medical technology, and in particular to a special device for fixing large tissue slices of a prostate whole organ and a manufacturing method thereof. Background Art

[0002] Patients with prostate cancer prefer radical prostatectomy. After surgery, in order to better conduct a comprehensive three-dimensional assessment of the lesions, some patients need to undergo large prostate tissue slicing. Since prostate cancer mostly occurs in elderly men, and most elderly men have more or less prostate hyperplasia, this results in different forms of prostate. Large prostate tissue slicing technology is to cut the whole organ prostate into 3mm thick slices after surgery, and then place the slices in order for fixation, embedding, and other steps to finally make a pathological report. It can be seen that fixation is not only the first step but also the most critical step in all procedures. For prostates that are relatively regular and relatively intact after surgery, they are cut into slices along the urethra and placed in order in this device for direct fixation. For irregular and scattered large tissues of the prostate after surgery, in order to prevent tissue loss, the same layer can be divided into two or more pieces of tissue due to irregularity and placed in the same fixed grid for fixation. The slices are compressed and fixed with a flat plate with mesh to avoid tissue curling and deformation. In order to prevent affecting the slice fixation effect, the holes must be dense and numerous to ensure that the liquid can fully contact the tissue slices.

[0003] Compared with other routine tissues after surgery, the whole organ of the prostate is not only large in size but also has higher technical requirements. In order to obtain better and complete sections, the step of tissue fixation is particularly important. If the tissue is not fixed well, the cut paraffin sections will fall off during the staining process, or even cannot be cut during slicing, which will directly affect the results of the report. If the tissue is fixed well, it will provide good technical support for the next step of machine operation, paraffin embedding, and paraffin sectioning, and provide technical support for the final preparation of complete prostate paraffin sections. It can be seen that a good fixation device is particularly important. Summary of the invention

[0004] The present invention aims to solve the technical problems in medical treatment at least to a certain extent. To this end, the present invention provides a special prostate whole organ large tissue slice fixing device and a manufacturing method.

[0005] The technical solution adopted by the present invention to solve its technical problems is: a special device for fixing large tissue slices of the whole prostate organ, which specifically includes a base box for placing tissues, a top cover box with a pressure-applying function, and a closed box for placing a fixing frame to fix the fixing liquid, the base box is a rectangular box-shaped structure, a base part with a plate structure with mesh holes is arranged in the base box, a connecting component for fixing the top cover box is arranged on the side of the base part, and a buckle component for clamping the top cover box is arranged on the other side of the base part, the top cover box is a box body with the same area, and a plate structure with mesh holes arranged vertically and horizontally is also arranged on the top cover box, the closed box is a rectangular box-shaped structure, and the length, width and height of the closed box are all larger than the base box.

[0006] In a preferred embodiment of the present invention, first traction handles are provided on both sides of the chassis box, and second traction handles are provided on the sides of the closed box.

[0007] In a preferred embodiment of the present invention, the connecting assembly includes a hinge block and a hinge rod for fixing the top cover with a set interval, the hinge block is arranged on the chassis box, the hinge rod is arranged on the top cover box, and a buckle device with elastic fixing and pressure-applying function is arranged in front of the chassis part, the buckle devices are arranged at intervals, the buckle device includes a buckle and a buckle groove, the buckle is arranged on the chassis box, and the buckle groove is arranged on the top cover box.

[0008] A special method for preparing large tissue slices of the whole prostate organ comprises the following steps:

[0009] Step 1: Pretreatment: First, fix the prostate specimen completely in formalin solution, then use dye to mark the left and right sides of the specimen, cut the left and right seminal vesicles, left and right vas deferens, 2mm at the apex of the prostate and 2mm at the base for routine pathological examination;

[0010] Step 2: Slice, photograph and record: Then put the prostate into the slicing box and slice it evenly into 3mm thin layers along the direction perpendicular to the urethra. Use numbers to mark the direction from the tip to the base, take photos and keep them to avoid confusion of the specimens;

[0011] Step 3: Tissue fixation: Put the cut thin layers of prostate tissue into the formalin solution in the bottom box and cover the top box; then put it in a sealed box for preliminary fixation and determine the fixation time according to the tissue shrinkage correction factor;

[0012] Step 4: Dehydration and wax dipping: Place the preliminarily fixed tissue into the machine for routine dehydration and wax dipping;

[0013] Step 5: Embedding and marking: embed the dehydrated wax-soaked tissues in sequence using a square box mold specifically for large-volume tissues. Place the embedded wax blocks in the same order as before and mark them with numbers after the wax blocks cool down.

[0014] Step 6: Slice: Slice the cooled large tissue wax block into 2.5-4um complete slices of whole-organ prostate tissue using a large tissue-specific slicer;

[0015] Step 7: Slicing: After the routine slicing, baking and drying, secondary dehydration, wax melting, HE staining, coverslipping, and finally slicing, a complete large tissue slide is prepared for the pathologist to diagnose the spatial distribution of prostate tissue.

[0016] In a preferred embodiment of the present invention, blade grooves are arranged on the upper and lower sides of the slicing box, and elastic compression components are arranged on the left and right sides of the slicing box. The elastic compression components include a compression plate and a spring. The spring is arranged on the left and right sides of the slicing box, and the compression plate is connected to the spring. The compression plate abuts against the prostate to be resected. A slicing cover is arranged on the slicing box, and a second blade groove is arranged on the slicing cover.

[0017] In a preferred embodiment of the present invention, during the pretreatment, the prostate specimen is completely fixed in a formalin solution with a volume fraction of 10% for 24-48 hours.

[0018] In a preferred embodiment of the present invention, in the tissue fixation process, the thin layer of prostate tissue is placed in a formalin solution with a volume fraction of 10% for fixation, and the specific fixation time is determined according to the shrinkage degree of the tissue after fixation.

[0019] In a preferred embodiment of the present invention, during the tissue fixation process, the tissue shrinkage correction factor is calculated by studying and measuring the volume changes of the prostate glands of multiple patients before and after the treatment.

[0020] The beneficial effects of the present invention are as follows: after adopting the above structure, the invention patent solves the problems that in the past, when large prostate tissue slices were fixed, the tissues were arranged in order, but the order was disordered after being fixed with the fixative; the tissues curled and deformed during fixation; the fixation was not sufficient during fixation, and the fixation effect was not good; the fixation time was long, and so on; this device not only solves the problems of disordered order and long fixation time, saving time and cost; it also allows the fixative to be fully fixed to each surface, so that the next step of the procedure can be successfully carried out; it also has a pressurization function, which can avoid tissue curling and deformation, so that the surface of the tissue after paraffin embedding and slicing is complete and smooth; it also reduces the loss of fragmented tissue; more importantly, this device can be directly placed in the machine for secondary fixation, solving the problem of having to re-arrange and take out the tissue for fixation in the past. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a cross-section of a prostate specimen;

[0022] Figure 2 This is a panoramic scan of the whole prostate organ specimen with gross staining;

[0023] Figure 3 This is a panoramic scan of the entire prostate organ specimen;

[0024] Figure 4 The tissue shrinkage correction factor calculation diagram for multiple patients with an average tissue shrinkage of 1.25;

[0025] Figure 5 A stereoscopic diagram of a slice fixing device;

[0026] Figure 6 A three-dimensional diagram of the chassis box of the slice fixing device;

[0027] Figure 7 This is a bottom view of the top cover box of the slice fixing device;

[0028] Figure 8 It is a top view of the slice box of the slice fixing device;

[0029] Fig. 9 Bottom view of the slice cover of the slice fixing device;

[0030] In the figure: 1, chassis box, 2, top cover box, 3, sealed box, 4, first traction handle, 5, second traction handle, 6, hinge block, 7, hinge rod, 8, buckle, 9, buckle slot, 10, slice box, 11, blade slot, 12, pressure plate, 13, spring, 14, slice cover, 15, second blade slot. DETAILED DESCRIPTION

[0031] The embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0032] like Figure 5-7As shown; a special device for fixing large tissue slices of the whole prostate organ, the special device for fixing large tissue slices of the whole prostate organ specifically comprises a bottom plate box 1 for placing tissues, a top cover box 2 with a pressure-applying function and a sealed box 3 for placing a fixing frame for fixing the fixing liquid, the bottom plate box 1 is a rectangular box-shaped structure, a bottom plate part with a plate structure with mesh holes is arranged inside the bottom plate box 1, a connecting component for fixing the top cover box 2 is arranged on the side of the bottom plate part, and a buckle component for clamping the top cover box 2 is arranged on the other side of the bottom plate part, the top cover box 2 is a box body with the same area, and a plate structure with mesh holes arranged vertically and horizontally is also arranged on the top cover box 2, the sealed box 3 is a rectangular box-shaped structure, and the length, width and height of the sealed box 3 are all larger than the bottom plate box 1; in the specific implementation, the bottom plate box 1 and the top cover box 2 are made of materials that are resistant to high temperature and high pressure, corrosion-resistant, repeatable, and insoluble in alcohol, xylene and paraffin, etc.,

[0033] like Figure 5 As shown, first traction handles 4 are arranged on both sides of the chassis box 1, and second traction handles 5 are arranged on the sides of the closed box 3.

[0034] like Figure 5-7 As shown, the connecting assembly includes a hinge block 6 and a hinge rod 7 for fixing the top cover with a set interval, the hinge block 6 is arranged on the chassis box 1, the hinge rod 7 is arranged on the top cover box 2, and a buckle device with elastic fixing and pressure-applying function is arranged in front of the chassis part, the buckle device is arranged at intervals, and the buckle device includes a buckle 8 and a buckle groove 9, the buckle 8 is arranged on the chassis box 1, and the buckle groove 9 is arranged on the top cover box 2; in a specific implementation, the buckle device in front of the top cover box 2 coincides with the buckle device in front of the bottom, and a pressure-applying effect is achieved after locking.

[0035] like Figure 1-4 As shown, a special method for preparing large tissue slices of the whole prostate organ comprises the following steps:

[0036] Step 1: Pretreatment: First, fix the prostate specimen completely in a 10% formalin solution for 30 hours, then mark the left and right sides of the specimen with dye, and cut the left and right seminal vesicles, left and right vas deferens, 2mm of the prostate tip and 2mm of the base for routine pathological examination;

[0037] Step 2: Slice, photograph and record: Then put the prostate into the slicing box 10 and evenly slice the prostate into 3 mm thin layers along the direction perpendicular to the urethra, mark them with numbers in sequence from the tip to the base, take photos and keep them to avoid confusion of the specimens;

[0038] Step 3, tissue fixation: the prostate thin layer tissue cut in sequence is placed in the bottom box 1 and placed in a 10% formalin solution by volume for fixation for 50 hours and then covered with the top cover box 2; then placed in the airtight box 3 for preliminary fixation; the fixation time is determined according to the tissue shrinkage correction factor of 1.25;

[0039] Step 4: Dehydration and wax dipping: Place the preliminarily fixed tissue into the machine for routine dehydration and wax dipping;

[0040] Step 5: Embedding and marking: embed the dehydrated wax-soaked tissues in sequence using a square box mold specifically for large-volume tissues. Place the embedded wax blocks in the same order as before and mark them with numbers after the wax blocks cool down.

[0041] Step 6: Slice: Slice the cooled large tissue wax block into 2.5-4um complete slices of whole-organ prostate tissue using a large tissue-specific slicer;

[0042] Step 7: Slicing: After the routine slicing, baking and drying, secondary dehydration, wax melting, HE staining, coverslipping, and finally slicing, a complete large tissue slide is prepared for the pathologist to diagnose the spatial distribution of prostate tissue.

[0043] In specific implementation, Figure 8-9 As shown, blade grooves 11 are arranged on the upper and lower sides of the slicing box 10, and elastic compression components are arranged on the left and right sides of the slicing box 10. The elastic compression components include a compression plate 12 and a spring 13. The spring 13 is arranged on the left and right sides of the slicing box 10, and the compression plate 12 is connected to the spring 13, and the compression plate 12 abuts against the prostate to be resected; a slicing cover 14 is arranged on the slicing box 10, and a second blade groove 15 is arranged on the slicing cover 14.

[0044] Embodiment 2:

[0045] like Figure 5-9 As shown; a special device for fixing large prostate whole organ tissue slices, the special device for fixing large prostate whole organ tissue slices specifically comprises a bottom plate box 1 for placing tissues, a top cover box 2 with a pressure-applying function, and a closed box 3 for placing a fixing frame for fixing with a fixing liquid,

[0046] like Figure 1-4 As shown, a special method for preparing large tissue slices of the whole prostate organ comprises the following steps:

[0047] Step 1: Pretreatment: First, fix the prostate specimen completely in a 10% formalin solution for 24 hours, then mark the left and right sides of the specimen with dye, and cut the left and right seminal vesicles, left and right vas deferens, 2mm at the apex of the prostate, and 2mm at the base for routine pathological examination;

[0048] Step 2, slice, take photos and record: Then put the prostate into the slice box and cut the prostate into 3mm thin layers evenly along the direction perpendicular to the urethra. The upper and lower sides of the slice box are provided with blade grooves, and the left and right sides of the slice box are provided with elastic compression components. The elastic compression components include compression plates and springs. The springs are provided on the left and right sides of the slice box. The compression plates are connected to the springs, and the compression plates abut against the prostate to be cut. Use digital marks in sequence from the tip to the base, take photos and keep them to avoid confusion of specimens.

[0049] Step 3: Tissue fixation: Place the sliced ​​prostate tissue in a tray box and fix it in a 10% formalin solution. Figure 4 As shown, the fixation time was determined based on a tissue shrinkage correction factor of 1.25;

[0050] Step 4: Dehydration and wax dipping: Place the preliminarily fixed tissue into the machine for routine dehydration and wax dipping;

[0051] Step 5: Embedding and marking: embed the dehydrated wax-soaked tissues in sequence using a square box mold specifically for large-volume tissues. Place the embedded wax blocks in the same order as before and mark them with numbers after the wax blocks cool down.

[0052] Step 6: Slice: Slice the cooled large tissue wax block into 2.5-4um complete slices of whole-organ prostate tissue using a large tissue-specific slicer;

[0053] Step 7: Slicing: After the routine slicing, baking and drying, secondary dehydration, wax melting, HE staining, coverslipping, and finally slicing, a complete large tissue slide is prepared for the pathologist to diagnose the spatial distribution of prostate tissue.

[0054] In specific implementation, the prostate whole organ large tissue slice fixing device is composed of a bottom and a top cover that are resistant to high temperature and high pressure, corrosion-resistant, repeatable, and insoluble in alcohol, xylene, paraffin, etc. It has the functions of fixation with fixative at room temperature and secondary fixation, wax immersion, and dehydration by a tissue fixer. Through fixation, the fixative can be well fixed to each surface of each tissue; in addition, the device has the function of pressurizing and flattening the tissue to prevent curling and deformation of the tissue, so that each piece of fixed tissue is flat so that a smooth and complete wax block can be embedded in the next step, and a strong guarantee is provided for the next step of slicing. After fixation, it not only lays a technical support for embedding and slicing, but also can guarantee the final complete pathology report.

[0055] In the description of this specification, the description with reference to the terms "one embodiment", "certain embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0056] In summary, although the present invention has been disclosed as above in terms of preferred embodiments, the above preferred embodiments are not intended to limit the present invention. A person skilled in the art may make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention shall be based on the scope defined in the claims.

Claims

1. A special device for fixing whole prostate organ large tissue slices, characterized by: The special device for fixing large tissue slices of the whole prostate organ specifically comprises a chassis box for placing tissues, a top cover box with a pressure-applying function, and a closed box for placing a fixing frame for fixing with a fixing liquid. The chassis box is a rectangular box-shaped structure, a chassis portion having a plate structure with mesh holes is arranged inside the chassis box, a connecting component for fixing the top cover box is arranged on the side of the chassis portion, and a buckle component for clamping the top cover box is arranged on the other side of the chassis portion. The top cover box is a box body with the same area, and a plate structure with mesh holes arranged vertically and horizontally is also arranged on the top cover box. The closed box is a rectangular box-shaped structure, and the length, width, and height of the closed box are all larger than the chassis box.

2. According to claim 1, a special device for fixing whole prostate organ large tissue slices, characterized in that: A first traction handle is arranged on both sides of the chassis box, and a second traction handle is arranged on the side of the closed box.

3. According to claim 1, a special device for fixing whole prostate organ large tissue slices, characterized in that: The connecting assembly includes a hinge block and a hinge rod for fixing the top cover with a set interval, the hinge block is arranged on the chassis box, the hinge rod is arranged on the top cover box, and a buckle device with elastic fixing and pressure-applying function is arranged in front of the chassis part, the buckle devices are arranged at intervals, the buckle device includes a buckle and a buckle groove, the buckle is arranged on the chassis box, and the buckle groove is arranged on the top cover box.

4. A special method for preparing large whole-organ prostate tissue slices, applied to a special whole-organ prostate tissue slice fixing device as claimed in any one of claims 1 to 3, characterized in that: The steps include: Step 1: Pretreatment: First, fix the whole prostate organ specimen in formalin solution, then use dye to mark the left and right sides of the specimen, cut the left and right seminal vesicles, left and right vas deferens, 2mm at the apex of the prostate and 2mm at the base for routine pathological examination; Step 2: Slice, photograph and record: Then put the prostate into the slicing box and slice it evenly into 3mm thin layers along the direction perpendicular to the urethra. Use numbers to mark the direction from the tip to the base, take photos and keep them to avoid confusion of the specimens; Step 3: Tissue fixation: The thin layers of prostate tissue cut in sequence are placed in the formalin solution in the bottom box and covered with the top cover; then placed in a sealed box for preliminary fixation, and the fixation time is determined according to the tissue shrinkage correction factor; Step 4: Dehydration and wax dipping: Place the preliminarily fixed tissue into the machine for routine dehydration and wax dipping; Step 5: Embedding and marking: embed the dehydrated wax-soaked tissues in sequence using a square box mold specifically for large-volume tissues. Place the embedded wax blocks in the same order as before and mark them with numbers after the wax blocks cool down. Step 6: Slice: Slice the cooled large tissue wax block into 2.5-4um complete slices of whole-organ prostate tissue using a large tissue-specific slicer; Step 7: Slicing: After the routine slicing, baking and drying, secondary dehydration, wax melting, HE staining, coverslipping, and finally slicing, a complete large tissue slide is prepared for the pathologist to diagnose the spatial distribution of prostate tissue.

5. A special method for preparing large prostate whole organ tissue slices according to claim 4, characterized in that: Blade grooves are arranged on the upper and lower sides of the slicing box, and elastic compression components are arranged on the left and right sides of the slicing box. The elastic compression components include a compression plate and a spring. The spring is arranged on the left and right sides of the slicing box, and the compression plate is connected to the spring. The compression plate abuts against the prostate to be resected. A slicing cover is arranged on the slicing box, and a second blade groove is arranged on the slicing cover.

6. A special method for preparing large prostate whole organ tissue slices according to claim 4, characterized in that: In the pretreatment, the prostate specimens were completely fixed in a 10% volume fraction formalin solution for 24-48 hours.

7. A special method for preparing large prostate whole organ tissue slices according to claim 4, characterized in that: During the tissue fixation process, a thin layer of prostate tissue is placed in a 10% by volume formalin solution for fixation, and the specific fixation time is determined according to the degree of shrinkage of the tissue after fixation.

8. A special method for preparing large prostate whole organ tissue slices according to claim 4, characterized in that: During tissue fixation, the tissue shrinkage correction factor was calculated by studying and measuring the volume changes of the prostate before and after treatment in multiple patients.

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