Embryo time-lapse culture system

Abstract

The application discloses an embryo time difference culture system and relates to the technical field of embryo culture. The system comprises an embryo culture chamber, an optical device, a three-dimensional motion device and a gas supply system. The optical device comprises an objective lens, a light source and a camera, and the objective lens, the light source and the camera are arranged on an optical platform. The upward parallel light emitted by the light source can pass through the embryo culture chamber and illuminate the embryo under the action of a light path reflection device. The three-dimensional motion device comprises a lifting mechanism for driving the optical platform to lift, a Y translation mechanism and an X translation mechanism. The gas supply system comprises a gas source for providing gas, a gas mixing device for mixing the gas and a gas supply device for supplying the gas to the embryo culture chamber. The gas is fully mixed by the gas mixing device, enters the gas supply device, and then returns to the gas mixing device. The embryo culture chamber, the optical device, the three-dimensional motion device and the gas supply system are connected with a control device. The system can improve the use effect of the embryo time difference culture device and simplify the structure of the device.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of embryo culture, and more particularly to an embryo time-lapse culture system. BACKGROUND

[0002] With the development of science and technology, time-lapse culture technology, as a new embryo culture technology, is more and more favored by clinical doctors and embryologists in the field of assisted reproduction at home and abroad. Since the time-lapse culture system was introduced in 2009, it has been used for more than 10 years, and has been used for the treatment of more than 1 million patients worldwide. Its safety and effectiveness in embryo in vitro culture have been confirmed. Compared with conventional culture technology, the application of time-lapse culture technology can greatly improve the pregnancy rate and live birth rate and reduce early miscarriage.

[0003] The embryo time-lapse culture system can culture embryos normally and take pictures of embryos at regular intervals to record morphological information during development, has the characteristics of non-human interference culture and real-time monitoring, and can dynamically observe embryos and evaluate them by using morphokinetic parameters, so that the screening results are more objective. The time-lapse culture system evaluates the development potential of embryos by time-lapse imaging technology, selects the best quality embryos for implantation, thereby effectively improving the clinical effect of assisted reproductive technology, increasing the implantation rate and clinical pregnancy rate, and shortening the time for successful pregnancy of patients.

[0004] Many companies and institutions have disclosed devices for embryo time-lapse culture. In the prior art, the devices have the mode of fixing a microscope and moving a culture dish for imaging, which requires constant movement of the sample, and the shaking during the movement may affect the development of the embryo. Or the devices have a disclosed device for monitoring the development of a sample, which adopts a combination mode of multiple completely independent culture modules, each independent culture module is provided with a microscope system and a motion mechanism, the cost is high, the overall size of the instrument is too large, and a large space is required.

[0005] In summary, how to improve the use effect of the embryo time-lapse culture device and simplify the structure of the device is a problem to be solved by those skilled in the art at present. SUMMARY

[0006] Therefore, the purpose of the present application is to provide an embryo time-lapse culture system, which can improve the use effect of the embryo time-lapse culture device and simplify the structure of the device.

[0007] In order to achieve the above-mentioned purpose, the present application provides the following technical solutions:

[0008] An embryo time-lapse culture system comprises:

[0009] An embryo culture chamber comprising an upper cover assembly and a lower cover assembly for sealingly connecting with the upper cover assembly, the upper cover assembly comprising a light path reflection device for reflecting a light path twice by 90 degrees to change upwardly irradiated parallel light into downwardly irradiated parallel light, and the lower cover assembly comprising a culture dish for accommodating a plurality of embryos;

[0010] An optical device comprising an objective lens for imaging an embryo, a light source for illuminating the objective lens, and a camera for photographing the embryo, the objective lens, the light source, and the camera being disposed on an optical platform, and the light source emitting upwardly parallel light which can pass through the embryo culture chamber and illuminate the embryo under the action of the light path reflection device;

[0011] A three-dimensional motion device comprising a lifting mechanism for driving the optical platform to lift, a Y translation mechanism for driving the lifting mechanism to move forward and backward along the Y axis, and an X translation mechanism for driving the Y translation mechanism to move left and right along the X axis, the lifting mechanism and the Y translation mechanism being vertically arranged, and the Y translation mechanism and the X translation mechanism being vertically arranged;

[0012] A gas supply system comprising a gas source for providing CO2 and N2, a gas mixing device for mixing the gas, and a gas supply device for supplying gas to the embryo culture chamber, the gas being fully mixed by the gas mixing device, entering the gas supply device, and then circulating in the gas mixing device;

[0013] A control device, the embryo culture chamber, the optical device, the three-dimensional motion device, and the gas supply system being connected with the control device.

[0014] Preferably, the upper cover assembly comprises a first temperature control device for real-time monitoring and control of the temperature of the upper cover assembly, and an upper cover housing for accommodating the light path reflection device and the first temperature control device;

[0015] The lower cover assembly comprises a gas path for communicating with the gas supply device, a second temperature control device for real-time monitoring and control of the temperature of the lower cover assembly, and a lower cover housing, the culture dish, the gas path, and the second temperature control device being disposed in the lower cover housing.

[0016] Preferably, the upper cover housing is provided with a rotary locking member for locking or releasing the lower cover housing;

[0017] The rotary locking member comprises a rotatable twist lock disposed through the upper cover housing, a knob portion being provided at the top of the twist lock for driving the twist lock to rotate, and a locking head being provided at the bottom of the twist lock, and the lower cover housing is provided with a waist-shaped hole for matingly clamping with the locking head;

[0018] The outer periphery of the turn lock is sequentially sleeved with a gasket, a nut and an oil-free bushing, the top of the gasket and the bottom of the oil-free bushing are respectively in abutment with the upper cover shell, and the bottom of the gasket and the top of the oil-free bushing are respectively in abutment with the two ends of the nut.

[0019] Preferably, the lower cover shell is provided with:

[0020] A light coupling seat;

[0021] A switch light coupling arranged in the light coupling seat;

[0022] A light coupling baffle arranged in the light coupling seat and corresponding to the locking head in position, when the locking head is in abutment with the light coupling baffle to deform the light coupling baffle, the light coupling baffle blocks the receiving light path of the switch light coupling; when the locking head is separated from the light coupling baffle, the light coupling baffle avoids the receiving light path.

[0023] Preferably, the light path reflection device comprises a first mirror distributed at an angle of 45° with the horizontal line, a second mirror distributed at an angle of 135° with the horizontal line, a third mirror arranged in parallel above the second mirror, a mirror seat for fixing the first mirror, and a double mirror seat for fixing the second mirror and the third mirror, the double mirror seat is provided with an L-shaped channel, and the second mirror and the third mirror are both provided with a condenser below.

[0024] Preferably, the gas path comprises a first opening hole arranged at the front end of the lower cover shell and a second opening hole arranged at the rear end of the lower cover shell, the first opening hole and the second opening hole are staggered and both are higher than the inner cavity bottom surface of the lower cover shell;

[0025] One end of a first connector is in communication with the first opening hole, the other end of the first connector is in communication with an air inlet pipe, one end of a second connector is in communication with the second opening hole, and the other end of the second connector is in communication with an air outlet pipe.

[0026] Preferably, the X translation mechanism comprises:

[0027] A bottom plate;

[0028] An X linear guide rail arranged in the X axis direction of the bottom plate and symmetrically arranged in double guide rails;

[0029] An X axis movement platform in sliding connection with the X linear guide rail and used for loading the Y translation mechanism;

[0030] A through-shaft type linear motor connected with the control device and arranged at the middle part of the X axis movement platform;

[0031] An X transmission screw rod connected with the through-shaft type linear motor and arranged above the bottom plate.

[0032] Preferably, the Y translation mechanism comprises:

[0033] a Y linear guide rail arranged symmetrically in pairs along the Y-axis direction of the X-axis motion platform;

[0034] a Y-axis motion platform slidably connected with the Y linear guide rail and configured to load the lifting mechanism;

[0035] a stepper motor connected with the control device and arranged at the middle of the Y-axis motion platform;

[0036] a Y transmission screw rod connected with the stepper motor and arranged above the X-axis motion platform;

[0037] a backlash nut fixed on the Y-axis motion platform and sleeved on the outer circumferential portion of the Y transmission screw rod.

[0038] Preferably, the lifting mechanism comprises:

[0039] a lifting seat arranged along the Z-axis direction of the Y-axis motion platform;

[0040] a lifting guide rail arranged symmetrically in pairs along the Z-axis direction of the lifting seat, the optical platform being slidably connected with the lifting guide rail;

[0041] a fixed-shaft linear motor connected with the control device and located on the side of the lifting seat away from the lifting guide rail, the output shaft of the fixed-shaft linear motor being in contact with the optical platform and transmitting motion;

[0042] a tension spring arranged vertically, the lifting seat and the optical platform being connected with the elastic ends of the tension spring.

[0043] Preferably, the gas source comprises a first compressed gas cylinder for loading CO2, a first mass flow controller connected with the first compressed gas cylinder, a second compressed gas cylinder for loading N2, and a second mass flow controller connected with the second compressed gas cylinder, the first mass flow controller and the second mass flow controller being connected with the control device.

[0044] Preferably, the gas mixing device comprises a premixing cavity for mixing gas, a concentration sensor for detecting gas concentration arranged in the premixing cavity, and a pressure relief valve for discharging excess gas, the concentration sensor and the pressure relief valve being connected with the control device.

[0045] The gas supply device comprises a one-way valve and a gas supply pump, the one-way valve is arranged between the gas outlet of the premixing cavity and the gas inlet of the embryo culture chamber, and the gas supply pump is arranged between the gas outlet of the embryo culture chamber and the gas return port of the premixing cavity, the one-way valve and the gas supply pump are arranged in one-to-one correspondence with the embryo culture chamber, and the gas supply pump is connected with the control device.

[0046] Preferably, the gas source further comprises a third compressed gas cylinder for loading premixed gas, a third pressure reducing valve in communication with the third compressed gas cylinder, a third filter in communication with the third pressure reducing valve, and a third mass flow controller connected with the third filter.

[0047] The third mass flow controller is in communication with the gas inlet of the premixing cavity, a two-position three-way reversing valve is arranged between the gas supply pump and the gas return port of the premixing cavity, and the two-position three-way reversing valve is connected with the control device to control the gas to return to the premixing cavity or directly discharge to the outside.

[0048] Preferably, a support is further arranged, the top of the support is provided with a plurality of embryo culture chambers arranged in a linear array, the arrangement direction of the embryos in the embryo culture chambers is the same as the arrangement direction of the embryo culture chambers, the optical device and the three-dimensional motion device are arranged at the bottom of the support, and the gas supply system is arranged at the side of the support.

[0049] When the embryo time difference culture system is used, the upper cover assembly can be opened to facilitate the placement of embryos into the culture dish, and then the upper cover assembly and the lower cover assembly are closed to facilitate the culture operation of the embryos. During the culture of the embryos, the gas supply system can be controlled to operate, so that the gas source provides CO2 and N2, the gas is fully mixed after passing through the gas mixing device, enters the gas supply device to supply gas to the embryo culture chamber, and provides the required gas environment for the embryos, and then the gas can be circulated in the gas mixing device.

[0050] When it is necessary to observe the culture of the embryos, the three-dimensional motion device can be controlled to operate to make the embryo to be measured reach a preset position, that is, the X translation mechanism, the Y translation mechanism and the lifting mechanism can be controlled to cooperate to operate to switch the position of the embryo and focus, wherein the X translation mechanism and the Y translation mechanism are responsible for positioning the embryo, and the lifting mechanism is responsible for switching the focusing plane of the embryo to facilitate the shooting of the images of different layers of the embryo along the Z axis direction.

[0051] When the embryo moves to the preset position for the shooting operation, the light beam emitted by the light source can pass through the embryo culture chamber, illuminate the embryo under the action of the light path reflecting device of the embryo culture chamber, and then be imaged through the objective lens and detected by the camera. The device sets the objective lens, light source and camera on the optical platform, without setting the running mechanism for the objective lens and light source respectively, which is beneficial to reduce the structural complexity and required space of the device.

[0052] In summary, the embryo time difference culture system provided by the application can improve the use effect of the embryo time difference culture device and simplify the structure of the device. BRIEF DESCRIPTION OF DRAWINGS

[0053] In order to more clearly illustrate the technical solutions in the embodiments of the application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description are only embodiments of the application, and for those skilled in the art, other drawings can also be obtained without creative labor on the basis of the provided drawings.

[0054] Figure 1 The structure diagram of the embryo time difference culture system provided by the application is shown in the figure.

[0055] Figure 2 The structure diagram of the embryo culture chamber is shown in the figure.

[0056] Figure 3 The structure diagram of the upper cover assembly is shown in the figure.

[0057] Figure 4 The structure diagram of the rotation locking piece is shown in the figure.

[0058] Figure 5 The structure diagram of the sealing piece is shown in the figure.

[0059] Figure 6 The structure diagram of the sealing connection between the upper cover assembly and the lower cover assembly is shown in the figure.

[0060] Figure 7 The structure diagram of the light path reflecting device is shown in the figure.

[0061] Figure 8 The structure diagram of the first temperature control device is shown in the figure.

[0062] Figure 9 The structure diagram of the lower cover assembly is shown in the figure.

[0063] Figure 10 The structure diagram of the light coupling seat, switch light coupling and light coupling baffle is shown in the figure.

[0064] Figure 11 The structure diagram of the culture dish is shown in the figure.

[0065] Figure 12 is a structural schematic diagram of the gas circuit;

[0066] Figure 13 is a structural schematic diagram of the second temperature control device;

[0067] Figure 14 is a structural schematic diagram of the three-dimensional movement device;

[0068] Figure 15 is a structural schematic diagram of the X translation mechanism;

[0069] Figure 16 is a structural schematic diagram of the Y translation mechanism;

[0070] Figure 17 is a structural schematic diagram of the lifting mechanism;

[0071] Figure 18 is a structural schematic diagram of the optical device;

[0072] Figure 19 is a schematic diagram of the optical path before the optical device moves to switch positions;

[0073] Figure 20 is a schematic diagram of the optical path after the optical device moves to switch positions;

[0074] Figure 21 is a structural schematic diagram of the gas supply system.

[0075] Figures 1-21 In the middle:

[0076] 1 is an embryo culture chamber, 11 is an upper cover assembly, 111 is an optical path reflecting device, 1111 is a first reflecting mirror, 1112 is a second reflecting mirror, 1113 is a third reflecting mirror, 1114 is a reflecting mirror seat, 1115 is a double reflecting mirror seat, 1116 is a condenser, 112 is a first temperature control device, 1121 is a heating belt, 1122 is a temperature sensor, 1123 is a pressing sheet, 113 is an upper cover shell, 114 is a rotary locking piece, 1141 is a rotating lock, 1142 is a knob part, 1143 is a locking head, 1144 is a gasket, 1145 is a nut, 1146 is an oil-free bushing, 115 is an outer pressing plate, 116 is an inner pressing plate, 117 is a sealing ring, 12 is a lower cover assembly, 121 is a culture dish, 1211 is a hole site, 122 is a gas circuit, 1221 is a first connector, 1222 is a second connector, 123 is a second temperature control device, 124 is a lower cover shell, 1241 is a waist-shaped hole, 125 is an optical coupling seat, 126 is a switch optical coupling, 127 is an optical coupling baffle, 2 is an optical device, 21 is an objective lens, 22 is a light source, 23 is a camera, 24 is an optical platform;

[0077] 3 is a three-dimensional motion device, 31 is a lifting mechanism, 311 is a lifting seat, 312 is a lifting guide rail, 313 is a fixed shaft type linear motor, 32 is a Y translation mechanism, 321 is a Y linear guide rail, 322 is a Y axis motion platform, 323 is a stepping motor, 324 is a Y transmission screw, 325 is an anti-backlash nut, 33 is an X translation mechanism, 331 is a bottom plate, 332 is an X linear guide rail, 333 is an X axis motion platform, 334 is a through shaft type linear motor, 335 is an X transmission screw, 4 is a gas supply system, 41 is a gas source, 411 is a first compressed gas cylinder, 412 is a first mass flow controller, 413 is a second compressed gas cylinder, 414 is a second mass flow controller, 415 is a third compressed gas cylinder, 416 is a third mass flow controller, 42 is a gas mixing device, 421 is a premixing cavity, 422 is a concentration sensor, 423 is a pressure relief valve, 43 is a gas supply device, 431 is a gas supply pump, 44 is a two-position three-way directional valve, 5 is a support, 6 is a control device. DETAILED DESCRIPTION

[0078] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0079] The core of the present application is to provide an embryo time difference culture system, which can improve the use effect of the embryo time difference culture device and simplify the structure of the device.

[0080] Please refer to Figures 1-21 .

[0081] The embodiment provides an embryo time difference culture system, which comprises:

[0082] The embryo culture chamber 1 comprises an upper cover assembly 11 and a lower cover assembly 12 used for sealingly connecting with the upper cover assembly 11, the upper cover assembly 11 comprises light path reflection devices 111 used for reflecting a light path by 90 degrees twice to change the upwardly irradiated parallel light into downwardly irradiated parallel light, and the lower cover assembly 12 comprises culture dishes 121 used for containing a plurality of embryos;

[0083] The optical device 2 comprises an objective lens 21 used for imaging the embryos, a light source 22 used for illuminating the objective lens 21 and a camera 23 used for photographing the embryos, the objective lens 21, the light source 22 and the camera 23 are all arranged on an optical platform 24, and the upwardly parallel light emitted by the light source 22 can pass through the embryo culture chamber 1 and illuminate the embryos under the action of the light path reflection devices 111;

[0084] The three-dimensional motion device 3 comprises a lifting mechanism 31 for driving the optical platform 24 to lift, a Y translation mechanism 32 for driving the lifting mechanism 31 to move back and forth along the Y axis, and an X translation mechanism 33 for driving the Y translation mechanism 32 to move left and right along the X axis, the lifting mechanism 31 and the Y translation mechanism 32 are vertically arranged, and the Y translation mechanism 32 and the X translation mechanism 33 are vertically arranged;

[0085] The gas supply system 4 comprises a gas source 41 for providing CO2 and N2, a gas mixing device 42 for mixing the gas, and a gas supply device 43 for supplying the gas to the embryo culture chamber 1, the gas is fully mixed by the gas mixing device 42, enters the gas supply device 43, and then circulates in the gas mixing device 42;

[0086] The control device 6 is connected with the embryo culture chamber 1, the optical device 2, the three-dimensional motion device 3, and the gas supply system 4.

[0087] It should be noted that the optical device 2 comprises the light source 22, the objective lens 21, and the camera 23, which are installed on the same optical platform 24, and the relative positions of the three are fixed, so that the light beam emitted by the light source 22 can pass through the embryo culture chamber 1 and illuminate the embryo under the action of the light path reflection device 111 in the embryo culture chamber 1, and then be imaged by the objective lens 21 and detected by the camera 23. The three-dimensional motion device 3 comprises the X translation mechanism 33, the Y translation mechanism 32, and the lifting mechanism 31, which are used to carry out position switching and focusing actions on the embryo by the optical device 2.

[0088] In actual application, the shape, structure, size, material, position, etc. of the embryo culture chamber 1, the optical device 2, the three-dimensional motion device 3, the gas supply system 4, and the control device 6 can be determined according to actual conditions and actual needs.

[0089] When using the embryo time difference culture system provided by the application, the upper cover assembly 11 can be opened to facilitate the placement of the embryo into the culture dish 121, and then the upper cover assembly 11 and the lower cover assembly 12 are closed to facilitate the culture operation of the embryo. During the culture of the embryo, the gas supply system 4 can be controlled to operate, so that the gas source 41 provides CO2 and N2, the gas is fully mixed by the gas mixing device 42, enters the gas supply device 43 to supply the gas to the embryo culture chamber 1, and provides the required gas environment for the embryo, and then the gas can circulate in the gas mixing device 42.

[0090] When it is needed to observe the culture condition of the embryo, the three-dimensional motion device 3 can be controlled to operate so as to make the embryo to be measured reach a preset position, that is, the X translation mechanism 33, the Y translation mechanism 32 and the lifting mechanism 31 are controlled to operate in cooperation, so as to perform position switching and focusing action on the embryo, wherein the X translation mechanism 33 and the Y translation mechanism 32 are responsible for position positioning of the embryo, and the lifting mechanism 31 is responsible for switching of the focusing plane of the embryo, so as to facilitate image shooting of different layers of the embryo along the Z axis direction.

[0091] When the embryo moves to the preset position for shooting operation, the light beam emitted by the light source 22 can pass through the embryo culture chamber 1, illuminate the embryo under the action of the light path reflection device 111 of the embryo culture chamber 1, and then be imaged by the objective 21 and detected by the camera 23. The device sets the objective 21, the light source 22 and the camera 23 on the optical platform 24, without the need to set the operation mechanism for the objective 21 and the light source 22 respectively, which is beneficial to reduce the structural complexity and the required space of the device.

[0092] In summary, the embryo time difference culture system provided by the application can improve the use effect of the embryo time difference culture device and simplify the structure of the device.

[0093] On the basis of the above embodiment, preferably, the upper cover assembly 11 comprises a first temperature control device 112 for real-time monitoring and control of the temperature of the upper cover assembly 11 and an upper cover shell 113 for accommodating the light path reflection device 111 and the first temperature control device 112.

[0094] The lower cover assembly 12 comprises a gas path 122 for communication with the gas supply device 43, a second temperature control device 123 for real-time monitoring and control of the temperature of the lower cover assembly 12 and a lower cover shell 124, wherein the culture dish 121, the gas path 122 and the second temperature control device 123 are arranged in the lower cover shell 124.

[0095] It should be noted that when the embryo culture chamber 1 is used, the upper cover shell 113 can be opened, so as to put the embryo into the culture dish 121 in the lower cover shell 124, and then the upper cover shell 113 and the lower cover shell 124 can be closed to be sealed, and then the first temperature control device 112 and the second temperature control device 123 can be controlled to operate, so as to ensure that the embryo is in a suitable temperature environment, and the gas path 122 and the gas supply system 4 can be controlled to operate, so as to provide the embryo with a required gas environment. When it is needed to observe and shoot the embryo, the light source 22 can be controlled to emit upward parallel light, which can be reflected twice by 90 degrees under the action of the light path reflection device 111, so as to make the upward parallel light become downward parallel light which is perpendicular to the imaging device, so as to expose the imaging device and facilitate observation and shooting operation of the imaging device.

[0096] Preferably, the upper cover shell 113 is provided with a rotary lock 1141 stopper 114 for locking or unlocking the lower cover shell 124;

[0097] The rotary lock 1141 stopper 114 comprises a rotatable turn lock 1141 provided through the upper cover shell 113, the top of the turn lock 1141 is provided with a knob part 1142 for driving the turn lock 1141 to rotate, the bottom of the turn lock 1141 is provided with a locking head 1143, and the lower cover shell 124 is provided with a waist-shaped hole 1241 for being clamped with the locking head 1143.

[0098] The outer circumferential part of the turn lock 1141 is sequentially sleeved with a gasket 1144, a nut 1145 and an oil-free bushing 1146, the top of the gasket 1144 and the bottom of the oil-free bushing 1146 are respectively abutted with the upper cover shell 113, and the bottom of the gasket 1144 and the top of the oil-free bushing 1146 are respectively abutted with both ends of the nut 1145.

[0099] It should be noted that the upper cover shell 113 and the lower cover shell 124 can be connected by a torque hinge, and the torque of the torque hinge can stop the upper cover shell 113 at any position when it is opened. When the opening operation of the upper cover shell 113 and the lower cover shell 124 is needed, the knob part 1142 can be rotated to drive the turn lock 1141 to rotate the locking head 1143, so that the locking head 1143 is separated from the waist-shaped hole 1241, and then the upper cover shell 113 and the lower cover shell 124 are easily opened; when the closing operation of the upper cover shell 113 and the lower cover shell 124 is needed, the knob part 1142 can be reversely rotated to drive the turn lock 1141 to reversely rotate the locking head 1143, so that the locking head 1143 is clamped with the waist-shaped hole 1241, and then the upper cover shell 113 and the lower cover shell 124 are locked and closed.

[0100] It should be further noted that two nuts 1145 can be arranged between the gasket 1144 and the oil-free bushing 1146, the nut 1145 is used to provide a pressing force to generate a friction force between the gasket 1144 and the upper cover shell 113, thereby generating a rotating damping, and the double-nut design can prevent the nut 1145 from loosening to reduce the pressing force and thereby reduce the rotating damping. The turn lock 1141 and the locking head 1143 are fixed by screws, and a groove for clamping the locking head 1143 can be arranged at the bottom end of the turn lock 1141 to effectively limit the locking head 1143, so as to avoid the relative rotation between the locking head 1143 and the turn lock 1141. During use, the turn lock 1141 can rotate relative to the upper cover shell 113 in the oil-free bushing 1146 to make the locking head 1143 and the waist-shaped hole 1241 clamped or separated, so as to realize the locking and unlocking operation of the upper cover shell 113 and the lower cover shell 124.

[0101] Preferably, the lower cover shell 124 is provided with:

[0102] optocoupler seat 125;

[0103] switch optocoupler 126, arranged in the optocoupler seat 125;

[0104] optocoupler baffle 127, arranged in the optocoupler seat 125, and the position of the optocoupler baffle 127 corresponds to the locking head 1143; when the locking head 1143 is pressed against the optocoupler baffle 127 to deform the optocoupler baffle 127, the optocoupler baffle 127 blocks the receiving light path of the switch optocoupler 126; when the locking head 1143 is separated from the optocoupler baffle 127, the optocoupler baffle 127 avoids the receiving light path.

[0105] It should be noted that when the locking head 1143 is pressed down, the optocoupler baffle 127 deforms to block the light path of the switch optocoupler 126 to achieve cover closing judgment; when the locking head 1143 is moved up, the optocoupler baffle 127 restores to not block the light path to achieve cover opening judgment. In the embryo culture process, the upper cover shell 113 and the lower cover shell 124 need to be tightly covered to provide the required temperature environment, gas environment, etc. for the embryo, and by setting the above-mentioned switch cover judgment mechanism, the operator can accurately judge whether the upper cover shell 113 and the lower cover shell 124 are completely closed, which is beneficial to improve the embryo culture effect.

[0106] Preferably, the light path reflection device 111 includes a first mirror 1111 distributed at an angle of 45° with the horizontal line, a second mirror 1112 distributed at an angle of 135° with the horizontal line, a third mirror 1113 arranged parallel above the second mirror 1112, a mirror seat 1114 for fixing the first mirror 1111, and a double mirror seat 1115 for fixing the second mirror 1112 and the third mirror 1113, the double mirror seat 1115 is provided with an L-shaped channel, and a condenser 1116 is horizontally arranged below the second mirror 1112 and the third mirror 1113. Figure 7 Figure 7 The arrow direction in the structure of the light path reflection device 111 is a schematic direction of the light path reflection route.

[0107] It should be noted that in the use process, the light source 22 emits vertical upward parallel light, and then the parallel light first passes through the first mirror 1111 and is refracted to the second mirror 1112. After the refraction of the second mirror 1112, it becomes vertical downward parallel light and is converged by the condenser 1116 below the second mirror 1112, and is directly irradiated on the culture dish 121 to realize the illumination operation.

[0108] ​Or the light source 22 emits vertical upward parallel light, and then the parallel light first passes through the first mirror 1111, then passes through the L-shaped channel and contacts the third mirror 1113, becomes vertical downward parallel light after the refraction of the third mirror 1113, and converges through the condenser 1116 below the third mirror 1113, and is directly irradiated on the culture dish 121 to realize the lighting operation.

[0109] On the basis of the above embodiment, preferably, the gas path 122 includes a first opening hole arranged at the front end of the lower cover shell 124 and a second opening hole arranged at the rear end of the lower cover shell 124, the first opening hole and the second opening hole are staggered and arranged, and both are higher than the inner cavity bottom surface of the lower cover shell 124; one end of the first connector 1221 is in communication with the first opening hole, the other end of the first connector 1221 is in communication with the gas inlet pipe, one end of the second connector 1222 is in communication with the second opening hole, and the other end of the second connector 1222 is in communication with the gas outlet pipe.

[0110] It should be noted that when the gas supply system 4 is running, the gas can sequentially pass through the gas inlet pipe, the first connector 1221, and the first opening hole, and then enter the lower cover shell 124 to provide the required gas environment for the embryo. In order to ensure the gas flowability, the gas can also sequentially pass through the second opening hole, the second connector 1222, and the gas outlet pipe to be discharged outward, so as to realize the gas exchange operation inside the device. Moreover, the front and rear gas path 122 opening holes of the lower cover shell 124 are slightly higher than the inner cavity bottom surface, in order to avoid liquid flowing into the gas path 122 when liquid is splashed out.

[0111] In addition, it should be noted that the first temperature control device 112 and the second temperature control device 123 each include a heating belt 1121, a temperature sensor 1122, and a pressing sheet 1123 for fixing the temperature sensor 1122, wherein the heating belt 1121 can be pasted on the upper cover shell 113 and the lower cover shell 124 by the back glue to heat the upper cover shell 113 and the lower cover shell 124. Moreover, the temperature sensor 1122 is pressed on the upper cover shell 113 and the lower cover shell 124 by the pressing sheet 1123 to monitor the temperature of the upper cover shell 113 and the lower cover shell 124 in real time, thereby realizing independent temperature control of the upper cover shell 113 and the lower cover shell 124. The sealed connection of the upper cover shell 113 and the lower cover shell 124 can mean that a sealing member for sealing the top of the lower cover shell 124 is arranged at the bottom of the upper cover shell 113, the sealing member includes an outer pressing plate 115, an inner pressing plate 116, and a sealing ring 117 clamped between the outer pressing plate 115 and the inner pressing plate 116, and the sealing ring 117 is provided with a mounting hole for mounting the glass sheet.

[0112] It should be further explained that the culture dish 121 can include two rows of culture grooves arranged in parallel, and eight hole positions 1211 are arranged at equal intervals in the culture grooves. The distance between adjacent hole positions 1211 is greater than the illumination diameter of parallel light, so as to increase the number of embryos cultured in the same culture dish 121, and realize the illumination observation of the two rows of embryos during the culture process. The distance between two adjacent hole positions 1211 is greater than the illumination diameter of parallel light, so that when one hole position 1211 is illuminated and observed, light will not be illuminated to the adjacent hole position, thereby avoiding affecting other embryos.

[0113] Preferably, the X translation mechanism 33 comprises:

[0114] a bottom plate 331;

[0115] an X linear guide rail 332 arranged symmetrically in double guide rails along the X-axis direction of the bottom plate 331;

[0116] an X-axis movement platform 333 connected with the X linear guide rail 332 for loading the Y translation mechanism 32;

[0117] a through-shaft type linear motor 334 connected with the control device 6 and arranged at the middle part of the X-axis movement platform 333;

[0118] an X transmission screw 335 connected with the through-shaft type linear motor 334 and arranged above the bottom plate 331.

[0119] Therefore, under the cooperation of the through-shaft type linear motor 334 and the X transmission screw 335, the X-axis movement platform 333 can be driven to move in the X-axis direction along the X linear guide rail 332.

[0120] Preferably, the Y translation mechanism 32 comprises:

[0121] a Y linear guide rail 321 arranged symmetrically in double guide rails along the Y-axis direction of the X-axis movement platform 333;

[0122] a Y-axis movement platform 322 connected with the Y linear guide rail 321 for loading the lifting mechanism 31;

[0123] a stepping motor 323 connected with the control device 6 and arranged at the middle part of the Y-axis movement platform 322;

[0124] a Y transmission screw 324 connected with the stepping motor 323 and arranged above the X-axis movement platform 333;

[0125] an anti-backlash nut 325 fixed on the Y-axis movement platform 322 and sleeved on the outer circumferential part of the Y transmission screw 324.

[0126] Therefore, under the transmission cooperation of the stepper motor 323, the Y transmission screw rod 324 and the anti-backlash nut 325, the Y-axis movement platform 322 can be driven to move in the Y-axis direction along the Y linear guide rail 321.

[0127] On the basis of the above embodiment, preferably, the lifting mechanism 31 comprises:

[0128] The lifting seat 311 is arranged along the Z-axis direction of the Y-axis movement platform 322.

[0129] The lifting guide rail 312 is arranged symmetrically in double guide rails along the Z-axis direction of the lifting seat 311, and the optical platform 24 is slidably connected with the lifting guide rail 312.

[0130] The fixed-shaft linear motor 313 is connected with the control device 6 and located on the side of the lifting seat 311 away from the lifting guide rail 312, the output shaft end of the fixed-shaft linear motor 313 is in contact with the optical platform 24 and transmits motion.

[0131] The tension spring is vertically distributed, and the lifting seat 311 and the optical platform 24 are connected with the elastic ends of the tension spring.

[0132] Therefore, under the contact between the output shaft end of the fixed-shaft linear motor 313 and the optical platform 24, the optical platform 24 can be pushed to move upward under the driving of the fixed-shaft linear motor 313, and can be reset and move downward under the gravity of the optical platform 24 or the action of the tension spring. The device can optimally control the structural size of the motion device and realize the compact design of the whole machine through the completely symmetrical layout design.

[0133] In addition, it should be noted that the optical module comprises the objective lens 21, the camera 23 and the light source 22, wherein the objective lens 21 and the light source 22 are installed on the same structural member and are arranged in parallel at a certain distance. When the embryos are photographed, the optical platform 24 can be moved to the first row of hole positions 1211 of the culture dish 121 along the Y-axis direction under the movement of the three-dimensional movement device 3, the light beam emitted by the light source 22 is deflected by 90 degrees after passing through the first reflector 1111, reaches the second reflector 1112, is deflected by 90 degrees again, and illuminates the first row of embryos in the culture dish 121, and the transmitted light beam is detected by the camera 23 after being collected by the objective lens 21. The optical platform 24 can be moved to the second row of hole positions 1211 along the Y-axis direction under the movement of the three-dimensional movement device 3, the light beam emitted by the light source 22 is deflected by 90 degrees after passing through the first reflector 1111, passes through the L-shaped channel and contacts the third reflector 1113, is deflected by 90 degrees again, and illuminates the second row of embryos in the culture dish 121, and the transmitted light beam is detected by the camera 23 after being collected by the objective lens 21, so as to realize the photographing of multiple rows of embryos.

[0134] Preferably, the gas source 41 comprises a first compressed gas cylinder 411 for loading CO2, a first mass flow controller 412 connected with the first compressed gas cylinder 411, a second compressed gas cylinder 413 for loading N2, and a second mass flow controller 414 connected with the second compressed gas cylinder 413, and the first and second mass flow controllers 412 and 414 are connected with the control device 6.

[0135] To improve the purity and delivery effect of the gas, a pressure reducing valve and a filter can be arranged between the compressed gas cylinder and the mass flow controller. For example, a first pressure reducing valve can be arranged in communication with the first compressed gas cylinder 411, a first filter can be arranged in communication with the first pressure reducing valve, and the first filter can be connected with the first mass flow controller 412. A second pressure reducing valve can be arranged in communication with the second compressed gas cylinder 413, a second filter can be arranged in communication with the second pressure reducing valve, and the second filter can be connected with the second mass flow controller 414. The control device 6 can control the operation of the first and second mass flow controllers 412 and 414 to control and adjust the gas flow of CO2 and N2.

[0136] Preferably, the gas mixing device 42 comprises a premixing chamber 421 in communication with the gas source 41 for mixing the gas, a concentration sensor 422 arranged in the premixing chamber 421 for detecting the concentration of the gas, and a pressure relief valve 423 for discharging excess gas, and the concentration sensor 422 and the pressure relief valve 423 are connected with the control device 6. The concentration sensor 422 can effectively detect the concentration of CO2 and O2, and the pressure relief valve 423 can discharge excess gas in time to maintain the pressure of the premixing chamber 421 stable. A one-way valve can be arranged between the first mass flow controller 412 and the premixing chamber 421, and between the second mass flow controller 414 and the premixing chamber 421.

[0137] The gas supply device 43 comprises a one-way valve and a gas supply pump 431, and a one-way valve is arranged between the gas outlet of the premixing chamber 421 and the gas inlet of the embryo culture chamber 1, and a gas supply pump 431 is arranged between the gas outlet of the embryo culture chamber 1 and the gas return inlet of the premixing chamber 421, and the one-way valve and the gas supply pump 431 are arranged one by one corresponding to the embryo culture chamber 1, and the gas supply pump 431 is connected with the control device 6. Therefore, under the action of the gas supply pump 431, the gas can enter the embryo culture chamber 1 from the premixing chamber 421 and return to the premixing chamber 421 to complete the circulation. During the gas supply process, the control device 6 can control the first and second mass flow controllers 412 and 414, the pressure relief valve 423, and the gas supply pump 431 to operate according to the detection signal of the concentration sensor 422, so as to provide the required gas to the embryo culture chamber 1 by the gas supply system 4.

[0138] On the basis of the above-mentioned embodiments, preferably, the gas source 41 further comprises a third compressed gas cylinder 415 for loading premixed gas, a third pressure reducing valve in communication with the third compressed gas cylinder 415, a third filter in communication with the third pressure reducing valve, and a third mass flow controller 416 connected with the third filter; the third mass flow controller 416 is in communication with the gas inlet of the premixing cavity 421, and a two-position three-way reversing valve 44 is arranged between the gas supply pump 431 and the gas return port of the premixing cavity 421, and the two-position three-way reversing valve 44 is connected with the control device 6 to control the return of the gas to the premixing cavity 421 or direct discharge to the outside.

[0139] It should be noted that by adding the third compressed gas cylinder 415, the third pressure reducing valve, the third filter, the third mass flow controller 416, and the two-position three-way reversing valve 44, the gas supply system 4 can also support the premixed gas supply mode. That is, when a mixture of multiple gases is needed, the first mass flow controller 412 and the second mass flow controller 414 can be opened to control the CO2 and N2 to enter the premixing cavity 421, thereby achieving the mixing of multiple gases and the dynamic adjustment of the CO2 and O2 concentrations; when premixed gas is needed, the third mass flow controller 416 can be opened to directly supply premixed gas to the embryo culture chamber 1. Therefore, the gas supply system 4 of the device is not a single gas supply mode, but supports premixed gas supply or three-gas supply, and the gas supply mode can be switched according to customer needs.

[0140] Preferably, the device further comprises a support 5, the top of the support 5 is provided with a plurality of embryo culture chambers 1 arranged in a linear array, the arrangement direction of the embryos in the embryo culture chambers 1 is the same as the arrangement direction of the embryo culture chambers 1, the optical device 2 and the three-dimensional motion device 3 are arranged at the bottom of the support 5, and the gas supply system 4 is arranged at the side of the support 5, so that the structure of the device is compact and occupies a small space. Among them, the X translation mechanism 33 and the Y translation mechanism 32 are responsible for positioning the linear array of embryo culture chambers 1.

[0141] It should be noted that the first compressed gas cylinder 411 and the second compressed gas cylinder 413 and the third compressed gas cylinder 415, the first pressure reducing valve and the second pressure reducing valve and the third pressure reducing valve, the first filter and the second filter and the third filter, the first mass flow controller 412 and the second mass flow controller 414 and the third mass flow controller 416, the first temperature control device 112 and the second temperature control device 123, the first mirror 1111 and the second mirror 1112 and the third mirror 1113, the first opening and the second opening, the first joint 1221 and the second joint 1222, among them, the first and the second and the third are only to distinguish the different positions, and there is no sequence.

[0142] In addition, it needs to be explained that the orientation or position relationship indicated by "X axis", "Y axis", "Z axis" and the like of the present application is based on the orientation or position relationship shown in the drawings, and is only for the convenience of simplifying the description and facilitating understanding, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present application.

[0143] The various embodiments in the specification are described in a progressive manner, and each embodiment focuses on the difference from other embodiments. The same or similar parts between the various embodiments can be referred to each other. Any combination of all the embodiments provided by the present application is within the protection scope of the present application, and is not described here.

[0144] The embryo time difference culture system provided by the present application is described in detail above. The principle and implementation manner of the present application are described by applying specific examples in this paper. The above description of the embodiments is only used to help understand the method of the present application and its core idea. It should be pointed out that for ordinary skilled persons in the technical field, some improvements and modifications can be made to the present application without departing from the principle of the present application, and these improvements and modifications also fall within the protection scope of the claims of the present application.

Claims

1. An embryonic time-lapse incubation system, characterized by, The application relates to an embryo culture chamber (1) comprising an upper cover assembly (11) and a lower cover assembly (12) used for sealing connection with the upper cover assembly (11), the upper cover assembly (11) comprising light path reflection devices (111) used for twice 90-degree reflection of a light path to change upwardly irradiated parallel light into downwardly irradiated parallel light, and the lower cover assembly (12) comprising a culture dish (121) used for containing a plurality of embryos; the upper cover assembly (11) comprises an upper cover shell (113) used for containing the light path reflection devices (111), the lower cover assembly (12) comprises a lower cover shell (124), the culture dish (121) is arranged in the lower cover shell (124), and the upper cover shell (113) is provided with a rotary locking piece (114) used for locking or loosening the lower cover shell (124); the rotary locking piece (114) comprises a rotatable turn lock (1141) penetrating through the upper cover shell (113), the top of the turn lock (1141) is provided with a knob part (1142) used for driving the turn lock (1141) to rotate, the bottom of the turn lock (1141) is provided with a locking head (1143), the lower cover shell (124) is provided with a waist-shaped hole (1241) used for clamping cooperation with the locking head (1143); the outer circumferential part of the turn lock (1141) is sequentially sleeved with a gasket (1144), a nut (1145) and an oil-free bushing (1146), the top of the gasket (1144) and the bottom of the oil-free bushing (1146) abut against the upper cover shell (113) respectively, and the bottom of the gasket (1144) and the top of the oil-free bushing (1146) abut against the two ends of the nut (1145) respectively; An optical device (2) comprises an objective lens (21) used for imaging embryos, a light source (22) used for illuminating the objective lens (21) and a camera (23) used for shooting embryos, the objective lens (21), the light source (22) and the camera (23) are arranged on an optical platform (24), and upwardly parallel light emitted by the light source (22) can pass through the embryo culture chamber (1) and illuminate embryos under the action of the light path reflection devices (111); A three-dimensional motion device (3) comprises a lifting mechanism (31) used for driving the optical platform (24) to lift, a Y translation mechanism (32) used for driving the lifting mechanism (31) to move back and forth along a Y-axis direction, and an X translation mechanism (33) used for driving the Y translation mechanism (32) to move left and right along an X-axis direction, the lifting mechanism (31) and the Y translation mechanism (32) are vertically arranged, and the Y translation mechanism (32) and the X translation mechanism (33) are vertically arranged. ​ The gas supply system (4) comprises a gas source (41) for providing CO2 and N2, a gas mixing device (42) for mixing the gas, and a gas supply device (43) for supplying gas to the embryo culture chamber (1), the gas being mixed sufficiently by the gas mixing device (42), entering the gas supply device (43), and then circulating in the gas mixing device (42); the gas mixing device (42) comprises a premixing cavity (421) for realizing gas mixing, a concentration sensor (422) for detecting the concentration of the gas, and a pressure relief valve (423) for discharging excess gas, which are arranged in the premixing cavity (421); the gas supply device (43) comprises a one-way valve and a gas supply pump (431), the one-way valve being arranged between the gas outlet of the premixing cavity (421) and the gas inlet of the embryo culture chamber (1), and the gas supply pump (431) being arranged between the gas outlet of the embryo culture chamber (1) and the gas return port of the premixing cavity (421), the one-way valve and the gas supply pump (431) being arranged in one-to-one correspondence with the embryo culture chamber (1). The control device (6) is connected with the embryo culture chamber (1), the optical device (2), the three-dimensional motion device (3), the concentration sensor (422), the pressure relief valve (423), and the gas supply pump (431).

2. The embryonic time-lapse incubation system of claim 1, wherein, The upper cover assembly (11) comprises a first temperature control device (112) for monitoring and controlling the temperature of the upper cover assembly (11) in real time, and the upper cover shell (113) is used for accommodating the first temperature control device (112). The lower cover assembly (12) comprises a gas path (122) for communicating with the gas supply device (43) and a second temperature control device (123) for monitoring and controlling the temperature of the lower cover assembly (12) in real time, and the gas path (122) and the second temperature control device (123) are arranged in the lower cover shell (124).

3. The embryonic time-lapse incubation system of claim 1, wherein, The lower cover shell (124) is provided with: a light coupling seat (125); a switch light coupling (126) arranged in the light coupling seat (125); a light coupling baffle (127) arranged in the light coupling seat (125), and the position of the light coupling baffle (127) corresponds to the locking head (1143), when the locking head (1143) is pressed against the light coupling baffle (127) to deform it, the light coupling baffle (127) blocks the receiving light path of the switch light coupling (126); when the locking head (1143) is separated from the light coupling baffle (127), the light coupling baffle (127) avoids the receiving light path.

4. The embryonic time-lapse incubation system of claim 1, wherein, The light path reflecting device (111) comprises a first mirror (1111) distributed at an angle of 45° with the horizontal line, a second mirror (1112) distributed at an angle of 135° with the horizontal line, a third mirror (1113) arranged in parallel above the second mirror (1112), a mirror seat (1114) for fixing the first mirror (1111), and a double mirror seat (1115) for fixing the second mirror (1112) and the third mirror (1113), wherein an L-shaped channel is arranged in the double mirror seat (1115), and a condenser (1116) is arranged horizontally below the second mirror (1112) and the third mirror (1113).

5. The embryonic time-lapse incubation system of claim 2, wherein, The gas path (122) comprises a first opening hole arranged at the front end of the lower cover shell (124) and a second opening hole arranged at the rear end of the lower cover shell (124), wherein the first opening hole and the second opening hole are arranged staggered and are both higher than the inner cavity bottom surface of the lower cover shell (124); One end of a first joint is communicated with the first opening hole, and the other end of the first joint is communicated with a gas inlet pipe; one end of a second joint is communicated with the second opening hole, and the other end of the second joint is communicated with a gas outlet pipe.

6. The embryonic time-lapse incubation system according to any one of claims 1 to 4, wherein, The X translation mechanism (33) comprises: a bottom plate (331); an X linear guide rail (332) arranged along the X-axis direction of the bottom plate (331) and symmetrically arranged in double guide rails; an X-axis movement platform (333) slidably connected with the X linear guide rail (332) and used for loading the Y translation mechanism (32); a through-shaft type linear motor (334) connected with the control device (6) and arranged at the middle part of the X-axis movement platform (333); and an X transmission screw (335) connected with the through-shaft type linear motor (334) and arranged above the bottom plate (331).

7. The embryonic time-lapse incubation system of claim 6, wherein, The Y translation mechanism (32) comprises: a Y linear guide rail (321) arranged along the Y-axis direction of the X-axis movement platform (333) and symmetrically arranged in double guide rails; a Y-axis movement platform (322) slidably connected with the Y linear guide rail (321) and used for loading the lifting mechanism (31); a stepping motor (323) connected with the control device (6) and arranged at the middle part of the Y-axis movement platform (322); a Y transmission screw (324) connected with the stepping motor (323) and arranged above the X-axis movement platform (333); and an anti-backlash nut (325) fixed on the Y-axis movement platform (322) and sleeved on the outer circumferential part of the Y transmission screw (324).

8. The embryonic time-lapse incubation system of claim 7, wherein, The lifting mechanism (31) comprises: a lifting seat (311) arranged along the Z-axis direction of the Y-axis movement platform (322); a lifting guide rail (312) arranged along the Z-axis direction of the lifting seat (311) and symmetrically arranged in double guide rails, wherein the optical platform (24) is slidably connected with the lifting guide rail (312); A fixed-shaft linear motor (313) is connected with the control device (6) and located on the side of the lifting seat (311) away from the lifting rail (312), and the output shaft end of the fixed-shaft linear motor (313) is in contact with and transmits motion to the optical platform (24). A pull spring is vertically distributed, and the lifting seat (311) and the optical platform (24) are connected with the elastic ends of the pull spring.

9. The embryonic time-lapse incubation system according to any one of claims 1 to 4, wherein, The gas source (41) comprises a first compressed gas cylinder (411) for loading CO2, a first mass flow controller (412) connected with the first compressed gas cylinder (411), a second compressed gas cylinder (413) for loading N2, and a second mass flow controller (414) connected with the second compressed gas cylinder (413), wherein the first mass flow controller (412) and the second mass flow controller (414) are connected with the control device (6).

10. The embryonic time-lapse incubation system of claim 1, wherein, The gas source (41) further comprises a third compressed gas cylinder (415) for loading premixed gas, a third pressure reducing valve in communication with the third compressed gas cylinder (415), a third filter in communication with the third pressure reducing valve, and a third mass flow controller (416) connected with the third filter. The third mass flow controller (416) is in communication with the gas inlet of the premixing cavity (421), a two-position three-way reversing valve (44) is arranged between the gas return outlet of the premixing cavity (421) and the gas supply pump (431), and the two-position three-way reversing valve (44) is connected with the control device (6) to control the return of gas to the premixing cavity (421) or direct discharge to the outside.

11. The embryonic time-lapse incubation system according to any one of claims 1 to 4, wherein, The support (5) is further provided with a plurality of embryo culture chambers (1) arranged in a linear array on the top of the support (5), the embryos in the embryo culture chambers (1) are arranged in the same direction as the embryo culture chambers (1), the optical device (2) and the three-dimensional motion device (3) are arranged on the bottom of the support (5), and the gas supply system (4) is arranged on the side of the support (5).

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