A method for plasma treatment of NO2 in model tissues - Quantitative measurement method system

Abstract

The invention belongs to the field of plasma technology and discloses a method for plasma treatment of NO2 in model tissue. — Quantitative measurement method for NO2 in plasma-treated model tissues — The quantitative measurement method includes: collecting color images of frozen sections of plasma-treated model tissues, establishing standard NO2 — A database corresponding to concentration and image; extracting RGB and HSV pixel components of the image, establishing the numerical relationship between concentration and pixel; obtaining plasma-treated model tissues treated with different plasma sources; measuring the penetration depth of the plasma-treated model tissues on the longitudinal section; and performing NO2 — The present invention can quickly and accurately measure the NO2 infiltrated into the model tissue. — The spatial distribution of concentration.

Description

Technical Field

[0001] The present invention belongs to the field of plasma technology, and in particular relates to a method for plasma treatment of NO2 in model tissues. - Quantitative measurement methods. Background Art

[0002] At present, the plasma dose problem involved in plasma treatment of biological tissues is one of the core issues in plasma biomedical applications. The liquid phase spectroscopy measurement and metallographic microscope measurement used in the industry have defects such as complex operation, high cost, and inability to measure the absolute spatial distribution. The plasma dose is directly related to the treatment outcome and is a key parameter for quantitative analysis of plasma source treatment of biological tissues. The quantitative measurement of active particles generated in plasma treatment of biological tissues has important guiding significance for medical applications and numerical simulations.

[0003] At present, the more unified view is that when plasma is used to treat biological tissues, the gaseous products such as O3, NO, and NO2 generated by the plasma source react with water molecules in the biological tissues to generate long-lived H2O2 and NO2 — 、NO3 — Particles such as NO2 play a major role in sterilization, especially in the NOx mode where NO2 is produced in large quantities — Particles, therefore, the quantitative diagnosis of these long-lived particles that penetrate into biological tissues is an important reference for plasma dose control and numerical simulation in medical applications. In addition, in experiments, model tissues such as gelatin and agar are usually used instead of biological tissues.

[0004] The core indicators of quantitative measurement include penetration depth, concentration and molar amount. The existing measurement methods usually use metallographic microscope for penetration depth, but this method is expensive and complicated to operate. Qualitative analysis and UV-visible spectrophotometry are usually used for concentration measurement. Qualitative analysis usually uses the color depth on the overall two-dimensional plane of the model tissue to qualitatively describe the relative size of the concentration, while UV-visible spectrophotometry is to use NO2 in the tissue to measure the relative concentration of NO2. — Particle extraction into liquid phase to measure average concentration or to measure uniformly distributed NO2 — concentration values; while no reports on absolute molar measurements have been reported so far.

[0005] Through the above analysis, the problems and defects of the prior art are as follows:

[0006] (1) Existing penetration depth measurement methods are costly and complex to operate;

[0007] (2) Existing measurement methods cannot accurately obtain absolute information on concentration or molar amount;

[0008] (3) Existing measurement methods cannot obtain NO2 that penetrates into the model tissue — Spatial distribution of concentration;

[0009] (4) The longitudinal section image of the treated model tissue has a small difference between the color and non-color images, making it difficult to accurately extract; the NO2 — The spatial distribution is difficult to observe and characterize. Summary of the invention

[0010] In view of the problems existing in the prior art, the present invention provides a method for plasma treatment of NO2 in model tissue. - Quantitative measurement methods.

[0011] The present invention is achieved by a method for plasma treatment of NO2 in model tissues. - Quantitative measurement method for NO2 in plasma treated model tissues - Quantitative measurement methods include:

[0012] Step 1: Establish standard NO2 by collecting color images of plasma-treated model tissue frozen sections — Database of concentration-image correspondences;

[0013] Step 2: extract the RGB and HSV pixel components of the image, establish a numerical relationship between concentration and pixel, and obtain plasma treatment model tissues treated by different plasma sources;

[0014] Step 3: Measure the penetration depth of the plasma-treated model tissue on the longitudinal section; — Concentration spatial distribution measurement and molar amount calculation.

[0015] Furthermore, in step 1, a standard NO2 — The database of concentration and image correspondence includes:

[0016] (1) Mix the plasma-treated model tissue with the color developing reagent and add different concentrations of NO2 — The solution is uniformly mixed with the plasma treated model tissue mixed with the color developing reagent to prepare a series of standard NO2 — concentration of plasma-treated model tissues;

[0017] (2) The obtained series of NO2 —After the plasma-treated model tissue with a concentration of 100 μg / cm2 was placed in a cryostat and frozen, each plasma-treated model tissue was cut into multiple frozen slices of fixed thickness. A digital camera was used to obtain a color image of each frozen slice of the plasma-treated model tissue at a fixed distance and under the same shooting environment to establish a standard NO2 — Database of concentration-to-image correspondences.

[0018] Furthermore, the color developing reagent is Gries reagent.

[0019] Furthermore, in step 2, extracting RGB and HSV pixel components of the image and establishing a numerical relationship between concentration and pixel includes:

[0020] The frozen section images of the plasma-treated model tissue were grayed, binarized, and contour-fitted to extract the section area in the image;

[0021] The R, G, B, and H pixel components of the slice area are extracted according to the RGB and HSV color spaces of the image, and a numerical relationship between the concentration and the R, G, B, and H pixels is established.

[0022] Further, extracting R, G, B, H pixel components of the slice area according to the RGB and HSV color spaces of the image and establishing a numerical relationship between the concentration and the R, G, B, H pixels includes:

[0023] The average values ​​of R, G, B, and H pixels in the slice area of ​​each image were calculated. The H value was used as the segmented fitting condition, and the concentration values ​​under different H segments were subjected to multivariate linear fitting with R, G, and B to obtain the numerical relationship between concentration and pixels.

[0024] The calculation formula of the concentration value C under different H segments is as follows:

[0025]

[0026] Further, in the step 2, obtaining plasma treatment model tissues treated by different plasma sources includes:

[0027] The plasma-treated model tissue mixed with the color developing reagent is treated by using jet, surface discharge or other plasma sources at different distances, different flow rates or different time conditions to obtain the plasma-treated model tissue treated by different plasma sources.

[0028] Furthermore, in step 3, measuring the penetration depth on the longitudinal section of the plasma-treated model tissue after treatment includes:

[0029] 1) obtaining plasma-treated model tissues treated with different plasma sources, and cutting the plasma-treated model tissues treated with different plasma sources from a symmetry line;

[0030] 2) using a digital camera to take longitudinal section images of the plasma-treated model tissues treated by the different plasma sources at a fixed distance;

[0031] 3) Grayscale, binarization, and logic operation processing are performed on the longitudinal section image to extract the pixel length of the penetration depth of the longitudinal section image, and then multiply it by the ratio between the pixel size and the actual size at a fixed distance to obtain the actual penetration depth.

[0032] Further, the step 3) comprises:

[0033] 3.1) Map the longitudinal section image to the HSV space, and extract the plasma treatment model tissue area image mask from the longitudinal section image according to the HSV color space features:

[0034]

[0035] Among them, (H (i,j) ,S (i,j) ,V (i,j) ) represents the HSV pixel value corresponding to the longitudinal section image at position (i, j); (H, S, V) color_low and (H, S, V) color_high Indicates the lower and upper limits of the HSV pixel corresponding to a specific color;

[0036] 3.2) grayscale and binarize the longitudinal section image corresponding to the mask area of ​​the image to obtain the uncolored area image of the model tissue;

[0037] 3.3) After negating the uncolored image, perform AND operation with the mask to obtain the penetration image:

[0038]

[0039] 3.4) Count the number of white pixels in each column of the penetration area image penetration to obtain the pixel size of the penetration depth in the radial direction. The pixel size is multiplied by the ratio coefficient k2 of the corresponding pixel size to the actual size when the vertical distance between the digital camera and the longitudinal section is fixed at d2 to obtain the actual penetration depth in the radial direction:

[0040] Depth actual =Depth pix *k2.

[0041] Further, in step 3, the plasma treated model tissue is subjected to NO2 —Concentration spatial distribution measurements and molar mass calculations include:

[0042] First, obtaining a plasma-treated model tissue after plasma treatment, freezing the plasma-treated model tissue after plasma treatment in a freezing microtome, and cutting the plasma-treated model tissue into a plurality of frozen sections of the same fixed thickness until the color-developed area is cut completely;

[0043] Secondly, a digital camera was used to obtain images of each frozen section of the plasma-treated model tissue at a fixed distance and under the same shooting environment; the slice area of ​​each frozen section color image was extracted, the R, G, B, and H pixel values ​​of each pixel point in the slice area were extracted, and the numerical relationship between concentration and pixel was calculated, and the concentration of each pixel point in each slice area was measured to obtain NO2 — Spatial distribution of concentration;

[0044] Finally, according to the obtained NO2 — The concentration is combined with the ratio of the pixel area to the actual area at a fixed distance, the number of pixels in the slice area, and the slice thickness to obtain the NO2 — molar amount, the NO2 — The total NO2 in the whole plasma treated model tissue is obtained by adding up the molar amounts. — Molar amount.

[0045] Further, in step 3, the plasma treated model tissue is subjected to NO2 - The concentration spatial distribution measurement and molar amount calculation include the following steps:

[0046] (1) obtaining a plasma-treated model tissue after plasma treatment, freezing the plasma-treated model tissue after plasma treatment in a freezing microtome, and cutting the plasma-treated model tissue into a plurality of frozen sections of the same fixed thickness until the color-developed area is cut completely;

[0047] (2) using a digital camera to obtain an image of each layer of frozen section of the plasma-treated model tissue at a fixed distance and under the same shooting environment; extracting a section area of ​​the image of each layer of frozen section of the plasma-treated model tissue;

[0048] (3) extracting the R, G, B, and H pixel components of the slice area, and calculating the concentration value corresponding to each pixel point of each slice area; based on the concentration value corresponding to each pixel point of each slice area, the actual molar amount of the corresponding pixel point is obtained using the following formula:

[0049]

[0050] Among them, C (i,j,k) represents the concentration of each pixel, (i, j) represents the pixel coordinates, and k represents the kth slice; S p It represents the actual area corresponding to each pixel when the vertical distance between the data camera and the slice is fixed at d1; h represents the slice thickness;

[0051] (4) Accumulate the molar amount of each pixel to obtain the total NO2 that penetrates into the plasma-treated model tissue. — Molar amount.

[0052] In combination with the above technical solutions and the technical problems solved, please analyze the advantages and positive effects of the technical solutions to be protected by the present invention from the following aspects:

[0053] First, in view of the technical problems existing in the above-mentioned prior art and the difficulty of solving the problems, the technical solutions to be protected by the present invention and the results and data during the research and development process are closely combined to analyze in detail and deeply how the technical solutions of the present invention solve the technical problems, and some creative technical effects brought about after solving the problems. The specific description is as follows:

[0054] The present invention uses frozen sectioning of model tissue and digital image processing technology to establish a numerical relationship between standard concentration and image pixels. After using a plasma source to treat the model tissue, the longitudinal section image of the model tissue is processed using image calculation to quickly obtain penetration depth parameters. The treated model tissue is frozen and sectioned, and combined with the relationship between the standard concentration and image pixels, the concentration and molar amount of each point in each layer of the model tissue can be obtained.

[0055] Second, considering the technical solution as a whole or from the perspective of the product, the technical effects and advantages of the technical solution to be protected by the present invention are described in detail as follows:

[0056] The present invention can quickly and accurately measure the NO2 infiltrated into the model tissue. — The spatial distribution of concentration.

[0057] Third, as auxiliary evidence of the inventiveness of the claims of the present invention, it is also reflected in the following important aspects: This technical method fills the gap in the domestic and foreign research on plasma treatment of NO2 in model tissues. — There is a gap in the measurement of parameters such as permeability concentration, spatial distribution, and absolute quantity. Previous methods could only obtain the overall average concentration and relative spatial distribution parameters, and were unable to quantitatively characterize the absolute quantity of internal parameters. The technical solution of the present invention solves the long-standing technical problems in plasma medical applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0058] Figure 1 The present invention provides a plasma treatment method for treating NO2 in a model tissue.— Schematic diagram of quantitative measurement method;

[0059] Figure 2 The present invention provides a plasma treatment method for treating NO2 in a model tissue. — Flow chart of quantitative measurement method;

[0060] Figure 3 is a flow chart of average color extraction of a slice region provided by an embodiment of the present invention;

[0061] Figure 4 is a schematic diagram of a calculation flow of a longitudinal section provided by an embodiment of the present invention;

[0062] Figure 5 is a schematic diagram of the variation trend of the penetration depth of a model tissue treated by a plasma jet according to an embodiment of the present invention;

[0063] Figure 6 It is a concentration distribution and molar amount diagram of each layer of the plasma jet treatment model tissue provided by an embodiment of the present invention;

[0064] Figure 7 is a schematic diagram of the variation trend of the penetration depth of the model tissue treated by surface discharge provided by an embodiment of the present invention;

[0065] Figure 8 It is a concentration distribution and molar amount diagram of each layer of the model organization of the surface discharge treatment provided by the embodiment of the present invention. DETAILED DESCRIPTION

[0066] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0067] 1. Explanatory Examples In order to enable those skilled in the art to fully understand how to implement the present invention, this section provides an illustrative example that expands and describes the technical solution of the claims.

[0068] like Figure 1-Figure 2 As shown, the plasma treatment of NO2 in the model tissue provided by the embodiment of the present invention — Quantitative measurement methods include:

[0069] S101, by collecting color images of plasma-treated model tissue frozen sections, establish standard NO2 — Database of concentration-image correspondences;

[0070] S102, extracting RGB and HSV pixel components of the image, establishing a numerical relationship between concentration and pixel; and obtaining plasma treatment model tissues treated by different plasma sources;

[0071] S103, measuring the penetration depth of the plasma-treated model tissue on the longitudinal section; and measuring the NO2 — Concentration spatial distribution measurement and molar amount calculation.

[0072] The present invention provides a plasma treatment method for treating NO2 in a model tissue. — Quantitative measurement methods include:

[0073] (1) Measurement of NO2 — The color developing reagent is Griess reagent, which is mixed with NO2 — The reaction generates a purple-red product. The higher the concentration, the darker the color. — The solution was evenly mixed with the model tissue mixed with Griess reagent to prepare a series of standard NO2 — Model tissue of concentration.

[0074] (2) A series of standard NO2 — The model tissue with the concentration was placed in a freezing microtome. After freezing, each standard NO2 — The model tissue sample with a concentration of 100 μm was cut into m frozen slices with a thickness of h, and each slice was photographed with a digital camera. The vertical distance between the digital camera and the slice was fixed at d1 to establish a standard NO2 — Database of concentration-to-image correspondences.

[0075] (3) The slice image is subjected to grayscale conversion, binarization, contour fitting and other operations to obtain the slice region image, and the R, G, B, and H pixel components of the slice region are extracted according to the RGB and HSV color spaces of the image.

[0076] (4) For each standard NO2 — For m images with different concentrations, the average values ​​of R, G, B, and H pixels in each image slice area are calculated. With H value as the segmented fitting condition, multivariate linear fitting is performed on the concentration value C and R, G, and B under different H segments:

[0077]

[0078] (5) Using plasma sources such as jet, surface discharge and other plasma sources, the model tissue mixed with Griess reagent is treated under different distances, different flow rates or different time conditions. Plasma treatment of the model tissue will produce NO2 inside it. — , NO2 —It reacts with Griess reagent to develop color.

[0079] (6) The plasma-treated model tissue is cut from the symmetry line, and the longitudinal section image is taken with a digital camera. The vertical distance between the digital camera and the longitudinal section is fixed at d2.

[0080] (7) Map the longitudinal section image to the HSV space, and extract the model tissue area image mask from the longitudinal section image based on the HSV color space features:

[0081]

[0082] Among them (H (i,j) ,S (i,j) ,V (i,j) ) represents the HSV pixel value corresponding to the longitudinal section image at position (i,j), (H,S,V) color_low and (H, S, V) color_high Indicates the lower and upper values ​​of the HSV pixel corresponding to a specific color.

[0083] (8) graying and binarizing the longitudinal section image corresponding to the mask area of ​​the image to obtain an uncolored area image of the model tissue;

[0084] (9) After negating uncolored, perform an AND operation with mask to obtain the penetration area image:

[0085]

[0086] (10) The number of white pixels in each column of the penetration area image is counted to obtain the pixel size of the penetration depth in the radial direction. The pixel size is multiplied by the ratio coefficient k2 of the corresponding pixel size to the actual size when the vertical distance between the digital camera and the longitudinal section is fixed at d2, and the actual penetration depth in the radial direction can be obtained.

[0087] (11) Count the number of white pixels in each column of the penetration area image to obtain the pixel size of the penetration depth along the radial direction. pix The actual penetration depth in the radial direction can be obtained by multiplying the pixel size by the ratio coefficient k2 of the corresponding pixel size to the actual size when the vertical distance between the digital camera and the longitudinal section is fixed at d2. actual :

[0088] Depth actual =Depth pix *k2 (4)

[0089] (12) The plasma-treated model tissue is placed in a freezing microtome, and after freezing, it is cut into n frozen sections with a thickness of h, until the colored area is cut, and each section is photographed using a digital camera, and the vertical distance between the digital camera and the section is fixed at d1.

[0090] (13) Extract the R, G, B, and H pixel components of the slice area according to the steps of the slice image and substitute them into formula (1) to obtain the concentration value C corresponding to each pixel point in each slice area. (i,j,k) , (i, j) represents the pixel coordinates, and k represents the k-th slice.

[0091] (14) Given the known concentration C of each pixel (i,j,k) After that, the actual molar amount n at that point can be deduced (i,j,k) :

[0092]

[0093] S p It represents the actual area corresponding to each pixel when the vertical distance between the data camera and the slice is fixed at d1, h is the slice thickness, and the total NO2 that penetrates into the model tissue can be obtained by accumulating the molar amount of each point — Molar amount.

[0094] The technical solution of the present invention is further described below in conjunction with specific embodiments.

[0095] Embodiment 1:

[0096] The present invention provides a plasma treatment method for treating NO2 in a model tissue. — The quantitative measurement method workflow is as follows Figure 1 shown.

[0097] Measuring NO2 — The color developing reagent used is Gries reagent. Here, Gries reagent is sufficient to react, NO2 — The higher the concentration, the deeper the color depth. — The solution was evenly mixed with the model tissue mixed with Griess reagent to prepare a series of standard NO2 — Model tissue of concentration, model tissue of configuration NO2 — The concentration gradient was set to {0, 50, 100, 150, 200, 400, 600, 800, 1000, 1500, 2000, 3000 μM}.

[0098] A series of standard NO2 — The model tissue with the concentration was placed in a freezing microtome. After freezing, each standard NO2 —The model tissue sample with a concentration of 100 μm was cut into 5 frozen sections, and each section was photographed with a digital camera. The vertical distance between the digital camera and the section was fixed at 25 cm to establish a standard NO2 — Database of concentration-to-image correspondences.

[0099] The slice image is processed by grayscale, binarization, contour fitting and other operations. The processing flow is as follows: Figure 3 As shown, a slice area image is obtained, the average color of the slice area image is calculated, and the average values ​​of the R, G, B, and H pixel components of the slice area are extracted according to the RGB and HSV color spaces of the image.

[0100] After obtaining a series of concentration values ​​and the average values ​​of R, G, B, and H pixel components, the H value is used as the segmented fitting condition, and multivariate linear fitting is performed on the concentration values ​​C and R, G, and B under different H segments. The fitting results are as follows:

[0101]

[0102] Using plasma jet, under different flow rates and different time conditions, the model tissue mixed with Gries reagent is treated. The plasma treatment of the model tissue will produce NO2 inside it. — , NO2 — It reacts with Griess reagent to develop color.

[0103] The model tissue treated by plasma jet was cut from the symmetry line, and the longitudinal section image was taken with a digital camera. The vertical distance between the digital camera and the longitudinal section was fixed at 25 cm.

[0104] For longitudinal images, Figure 4 The processing flow is to obtain the pixel size Depth of the penetration depth along the radial direction. pix The actual penetration depth in the radial direction can be obtained by multiplying the pixel size by the ratio coefficient 22.2 of the corresponding pixel size to the actual size when the vertical distance between the digital camera and the longitudinal section is fixed at 25 cm. actual The variation trend of penetration depth in radial direction is as follows: Figure 5 shown.

[0105] The plasma jet treated model tissue was placed in a freezing microtome, and after freezing, it was cut into 20-30 frozen sections with a thickness of 100 μm, until the color-developed area was cut, and each section was photographed using a digital camera, with the vertical distance between the digital camera and the section fixed at 25 cm.

[0106] The model tissue slice image after plasma jet treatment is Figure 3The R, G, B, and H pixel components of the slice area are extracted by the process and substituted into formula (6) to obtain the concentration value corresponding to each pixel point in each slice area. When the vertical distance between the data camera and the slice is fixed at 25 cm, the actual area corresponding to each pixel point is 0.0009 mm 2 According to formula (5), the molar amount of each pixel can be obtained, and the molar amount of each layer and the whole can be obtained by accumulation. The concentration distribution and molar amount of each layer of the plasma jet treatment model tissue are as follows: Figure 6 shown.

[0107] Embodiment 2:

[0108] The model tissue mixed with Gries reagent was treated by surface discharge at different distances and different times, using the same relationship between concentration and pixel value as in Example 1, i.e., formula (6), while the penetration depth, concentration and molar amount measurement process was similar to that of the plasma jet, and NO2 was measured. — The color developing reagent used is Gries reagent. Here, Gries reagent is sufficient to react, NO2 — The higher the concentration, the deeper the color depth. — The solution was evenly mixed with the model tissue mixed with Griess reagent to prepare a series of standard NO2 — Model tissue of concentration, model tissue of configuration NO2 — The concentration gradient was set to {0, 50, 100, 150, 200, 400, 600, 800, 1000, 1500, 2000, 3000 μM}.

[0109] A series of standard NO2 — The model tissue with the concentration was placed in a freezing microtome. After freezing, each standard NO2 — The model tissue sample with a concentration of 100 μm was cut into 5 frozen sections, and each section was photographed with a digital camera. The vertical distance between the digital camera and the section was fixed at 25 cm to establish a standard NO2 — Database of concentration-to-image correspondences.

[0110] The slice image is processed by grayscale, binarization, contour fitting and other operations. The processing flow is as follows: Figure 3 As shown, a slice area image is obtained, the average color of the slice area image is calculated, and the average values ​​of the R, G, B, and H pixel components of the slice area are extracted according to the RGB and HSV color spaces of the image.

[0111] After obtaining a series of concentration values ​​and the average values ​​of R, G, B, and H pixel components, the H value is used as the segmented fitting condition, and multivariate linear fitting is performed on the concentration values ​​C and R, G, and B under different H segments. The fitting results are as follows:

[0112]

[0113] Using plasma jet, under different flow rates and different time conditions, the model tissue mixed with Gries reagent is treated. The plasma treatment of the model tissue will produce NO2 inside it. — , NO2 — It reacts with Griess reagent to develop color.

[0114] The model tissue treated by plasma jet was cut from the symmetry line, and the longitudinal section image was taken with a digital camera. The vertical distance between the digital camera and the longitudinal section was fixed at 25 cm.

[0115] For longitudinal images, Figure 4 The processing flow is to obtain the pixel size Depth of the penetration depth along the radial direction. pix The actual penetration depth in the radial direction can be obtained by multiplying the pixel size by the ratio coefficient 22.2 of the corresponding pixel size to the actual size when the vertical distance between the digital camera and the longitudinal section is fixed at 25 cm. actual The variation trend of penetration depth in the radial direction of the surface discharge treatment model organization is as follows Figure 7 As shown, the concentration distribution and molar amount of each layer of the surface discharge treatment model organization are as follows Figure 8 shown.

[0116] 2. Evidence of the effects of the embodiments The embodiments of the present invention have achieved some positive effects during the development or use process, and indeed have great advantages over the prior art. The following content is described in conjunction with the data, charts, etc. of the test process.

[0117] In the above two embodiments, the average concentration in the plasma-treated model tissue is 400-500 μM, which is in the same order of magnitude as the 290 μM and 600 μM reported in the existing literature; the penetration depth is 0.4-1.6 mm, which is also in the same numerical range as the 0.5-1.5 mm and 0.5-2 mm reported in the existing literature. The measurement results have a reliable reference value in terms of the different plasma sources and model tissue compositions used.

[0118] It should be noted that the embodiments of the present invention can be implemented by hardware, software, or a combination of software and hardware. The hardware part can be implemented using dedicated logic; the software part can be stored in a memory and executed by an appropriate instruction execution system, such as a microprocessor or dedicated design hardware. It can be understood by a person of ordinary skill in the art that the above-mentioned devices and methods can be implemented using computer executable instructions and / or contained in a processor control code, such as a carrier medium such as a disk, CD or DVD-ROM, a programmable memory such as a read-only memory (firmware), or a data carrier such as an optical or electronic signal carrier. Such code is provided on the carrier medium. The device and its modules of the present invention can be implemented by hardware circuits such as very large-scale integrated circuits or gate arrays, semiconductors such as logic chips, transistors, etc., or programmable hardware devices such as field programmable gate arrays, programmable logic devices, etc., can also be implemented by software executed by various types of processors, and can also be implemented by a combination of the above-mentioned hardware circuits and software, such as firmware.

[0119] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Any modifications, equivalent substitutions and improvements made by any technician familiar with the technical field within the technical scope disclosed by the present invention and within the spirit and principle of the present invention should be covered by the protection scope of the present invention.

Claims

1. A method for plasma treatment of NO2 in model tissues - A quantitative measurement method, characterized in that The method for plasma treatment of NO2 in model tissue - Quantitative measurement methods include: Step 1: Establish standard NO2 by collecting color images of plasma-treated model tissue frozen sections - Database of concentration-image correspondences; Step 2: extract the RGB and HSV pixel components of the image, establish a numerical relationship between concentration and pixel, and obtain plasma treatment model tissues treated by different plasma sources; Step 3: Measure the penetration depth of the plasma-treated model tissue on the longitudinal section; - Concentration spatial distribution measurement and molar amount calculation; In the step 1, a color image of a frozen section of a plasma-treated model tissue is collected to establish a standard NO2 - The database of concentration and image correspondence includes: (1) Mix the plasma-treated model tissue with the color developing reagent and add different concentrations of NO2 - The solution is uniformly mixed with the plasma treated model tissue mixed with the color developing reagent to prepare a series of standard NO2 - concentration of plasma-treated model tissues; (2) The obtained series of NO2 - After the plasma-treated model tissue with a concentration of 100 μg / cm2 was placed in a cryostat and frozen, each plasma-treated model tissue was cut into multiple frozen slices of fixed thickness. A digital camera was used to obtain a color image of each frozen slice of the plasma-treated model tissue at a fixed distance and under the same shooting environment to establish a standard NO2 - Database of concentration-image correspondences; In step 3, the plasma treated model tissue is subjected to NO2 - Concentration spatial distribution measurements and molar mass calculations include: First, obtaining a plasma-treated model tissue after plasma treatment, freezing the plasma-treated model tissue after plasma treatment in a freezing microtome, and cutting the plasma-treated model tissue into a plurality of frozen sections of the same fixed thickness until the color-developed area is cut completely; Secondly, a digital camera was used to obtain images of each frozen section of the plasma-treated model tissue at a fixed distance and under the same shooting environment; the slice area of ​​each frozen section color image was extracted, the R, G, B, and H pixel values ​​of each pixel point in the slice area were extracted, and the numerical relationship between concentration and pixel was calculated, and the concentration of each pixel point in each slice area was measured to obtain NO2 - Spatial distribution of concentration; Finally, according to the obtained NO2 - The concentration is combined with the ratio of the pixel area to the actual area at a fixed distance, the number of pixels in the slice area, and the slice thickness to obtain the NO2 - molar amount, the NO2 - The total NO2 in the whole plasma treated model tissue is obtained by adding up the molar amounts. - Molar amount.

2. The method for plasma treating NO2 in a model tissue as claimed in claim 1 - A quantitative measurement method, characterized in that The color developing reagent is Griess reagent.

3. The method for treating NO2 in a model tissue using plasma as claimed in claim 1 - A quantitative measurement method, characterized in that In the step 2, extracting the RGB and HSV pixel components of the image and establishing a numerical relationship between the concentration and the pixel include: The frozen section images of the plasma-treated model tissue were grayed, binarized, and contour-fitted to extract the section area in the image; The R, G, B, and H pixel components of the slice area are extracted according to the RGB and HSV color spaces of the image, and a numerical relationship between the concentration and the R, G, B, and H pixels is established.

4. The method for treating NO2 in a model tissue by plasma treatment as claimed in claim 3 - A quantitative measurement method, characterized in that The extracting R, G, B, H pixel components of the slice area according to the RGB and HSV color spaces of the image and establishing the numerical relationship between the concentration and the R, G, B, H pixels includes: The average values ​​of R, G, B, and H pixels in the slice area of ​​each image were calculated. The H value was used as the segmented fitting condition, and the concentration values ​​under different H segments were subjected to multivariate linear fitting with R, G, and B to obtain the numerical relationship between concentration and pixels. The calculation formula of the concentration value C under different H segments is as follows:

5. The method for plasma treating NO2 in a model tissue as claimed in claim 1 - A quantitative measurement method, characterized in that In the step 2, obtaining plasma treatment model tissues treated by different plasma sources includes: The plasma-treated model tissue mixed with the color developing reagent is treated by using jet, surface discharge or other plasma sources at different distances, different flow rates or different time conditions to obtain the plasma-treated model tissue treated by different plasma sources.

6. The method for plasma treatment of NO2 in model tissue according to claim 1 - A quantitative measurement method, characterized in that In the step 3, measuring the penetration depth on the longitudinal section of the plasma-treated model tissue after treatment includes: 1) obtaining plasma-treated model tissues treated with different plasma sources, and cutting the plasma-treated model tissues treated with different plasma sources from a symmetry line; 2) using a digital camera to take longitudinal section images of the plasma-treated model tissues treated by the different plasma sources at a fixed distance; 3) Grayscale, binarization, and logic operation processing are performed on the longitudinal section image to extract the pixel length of the penetration depth of the longitudinal section image, and then multiply it by the ratio between the pixel size and the actual size at a fixed distance to obtain the actual penetration depth.

7. A method for treating NO2 in a model tissue using plasma as claimed in claim 6 - A quantitative measurement method, characterized in that The step 3) comprises: 3.1) Map the longitudinal section image to the HSV space, and extract the plasma treatment model tissue area image mask from the longitudinal section image according to the HSV color space features: Among them, (H (i,j) ,S (i,j) ,V (i,j) ) represents the HSV pixel value corresponding to the longitudinal section image at position (i, j); (H, S, V) color_low and (H, S, V) color_high Indicates the lower and upper limits of the HSV pixel corresponding to a specific color; 3.2) grayscale and binarize the longitudinal section image corresponding to the mask area of ​​the image to obtain the uncolored area image of the model tissue; 3.3) After negating the uncolored image, perform AND operation with the mask to obtain the penetration image: 3.4) Count the number of white pixels in each column of the penetration area image penetration to obtain the pixel size of the penetration depth in the radial direction. The pixel size is multiplied by the ratio coefficient k2 of the corresponding pixel size to the actual size when the vertical distance between the digital camera and the longitudinal section is fixed at d2 to obtain the actual penetration depth in the radial direction: Depth actual =Depth pix *k2。 8. The method for plasma treatment of NO2 in model tissues as claimed in claim 1 - A quantitative measurement method, characterized in that In step 3, the plasma treated model tissue is subjected to NO2 - The concentration spatial distribution measurement and molar amount calculation include the following steps: (1) obtaining a plasma-treated model tissue after plasma treatment, freezing the plasma-treated model tissue after plasma treatment in a freezing microtome, and cutting the plasma-treated model tissue into a plurality of frozen sections of the same fixed thickness until the color-developed area is cut completely; (2) using a digital camera to obtain an image of each layer of frozen section of the plasma-treated model tissue at a fixed distance and under the same shooting environment; extracting a section area of ​​the image of each layer of frozen section of the plasma-treated model tissue; (3) extracting the R, G, B, and H pixel components of the slice area, and calculating the concentration value corresponding to each pixel point of each slice area; based on the concentration value corresponding to each pixel point of each slice area, the actual molar amount of the corresponding pixel point is obtained using the following formula: n (i,j,k) =C (i,j,k) *S p *h Among them, C (i,j,k) represents the concentration of each pixel, (i, j) represents the pixel coordinates, and k represents the kth slice; S p It represents the actual area corresponding to each pixel when the vertical distance between the data camera and the slice is fixed at d1; h represents the slice thickness; (4) Accumulate the molar amount of each pixel to obtain the total NO2 that penetrates into the plasma-treated model tissue. - Molar amount.

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