A multi-channel microfluidic chip for parallel sampling

Abstract

The present invention discloses a multi-channel microfluidic chip for parallel injection, which is used for microfluidic control and includes: a substrate, at least two independent radial shunt channels, an injection capillary, and a central channel are provided on the substrate; the radial shunt channels and the injection capillary are connected to the side wall channels of the central channel; the injection capillary is used to input samples; the radial shunt channels are used to introduce the same solution at the same time, and radiate to the edge with the central channel as the center; the central channel is used to store samples, and the samples in each radial shunt channel are subjected to the same force under centrifugal action. The present invention can realize the simultaneous and equal volume introduction of sample solutions in multiple channels by designing the chip injection channel, realize high-throughput detection, and improve analysis efficiency; reduce the use of pumps and valves, and realize device miniaturization.

Description

Technical Field

[0001] The invention relates to the technical field of microfluidic chip liquid chromatography, and in particular to a multi-channel microfluidic chip for parallel injection. Background Art

[0002] Microfluidic chip liquid chromatography has the advantages of low sample reagent consumption, high separation efficiency, high throughput, miniaturization, and integration. It is an important way to miniaturize chromatographic instruments. Microfluidic chip liquid chromatography is to prepare a chromatographic column on a microfluidic chip and integrate the corresponding fluid control unit and detection unit; that is, the injection, separation, and detection units are integrated together. Among them, injection is an extremely important step in chromatographic analysis. At present, the injection method in microfluidic chip liquid chromatography can be divided into single-channel injection and multi-channel injection. For samples with a large number and complex components, single-channel injection analysis requires a lot of time and low analysis efficiency, so it is necessary to develop multi-channel injection to reduce injection time, improve efficiency, and achieve high-throughput detection. At present, multi-channel injection methods are all based on external pumps and valves to achieve multi-channel injection. In the prior art, multi-pumps are usually used to drive samples to different array columns to increase the parallel flux to improve the enrichment capacity, but multi-pump drive often occupies a large space and has poor stability. Alternatively, the sample after one-dimensional separation is introduced into the two-dimensional array column for separation through an external valve, but the use of an external valve will lead to the generation of dead volume and does not meet the requirements of integration and miniaturization of the chromatographic system. Since the microchannel is reproducible and can avoid the use of too many connectors and drive pumps, an array of multiple channels can be designed on the microfluidic chip to achieve parallel injection.

[0003] Therefore, it is necessary to study a chip-based multi-channel injection system that is miniaturized, has good stability, and can achieve parallel injection. Summary of the invention

[0004] The purpose of the present invention is to provide a multi-channel microfluidic chip for parallel injection to solve the problems existing in the above-mentioned prior art.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] A multi-channel microfluidic chip for parallel injection, comprising: a substrate, at least two independent radial flow-dividing channels opened on the substrate, an injection capillary, and a central channel;

[0007] The radial flow splitting channel and the injection capillary are both connected to the side wall channel of the central channel;

[0008] The injection capillary is used to input samples;

[0009] The radial flow-dividing channel is used for simultaneously introducing the same solution, and radiates toward the edge with the central channel as the center;

[0010] The central channel is used to store samples so that the samples are subjected to the same force in each radial flow channel during centrifugation.

[0011] Preferably, the entire chip is sealed by thermal bonding.

[0012] Furthermore, by adopting the above-mentioned sealing method, high-precision and high-efficiency sealing can be achieved.

[0013] Preferably, the central channel is a semicircular column structure;

[0014] The radial flow-dividing channel is connected to the side wall channel on one side of the semicircular shape of the central channel.

[0015] Furthermore, by adopting the above method, the sample solutions in multiple channels can be introduced simultaneously and in equal volumes, while the use of pumps and valves is reduced, thereby achieving miniaturization of the device.

[0016] Preferably, it also includes: a waste liquid end capillary;

[0017] The plurality of waste liquid end capillaries are adjacent to the injection capillary and are symmetrically arranged with the injection capillary as the axis;

[0018] The waste liquid end capillary is used to collect and discharge the reaction waste liquid.

[0019] Furthermore, by keeping the waste liquid end always closest to the radial split flow channel than the injection capillary, waste liquid can be collected accurately.

[0020] Preferably, the injection capillary and the waste liquid end capillary, the waste liquid end capillary and the radial diversion channel, and the connection between the radial diversion channels are all designed with an arc structure.

[0021] Furthermore, through the above arrangement, the sample can flow more smoothly in the microfluidic chip.

[0022] Preferably, the radial diversion channel is opened according to experimental requirements.

[0023] From the above content, it can be known that compared with the prior art, the beneficial effects of the present invention are:

[0024] The present invention can realize simultaneous and equal volume introduction of sample solutions in multiple channels through the design of chip injection channels, thereby achieving high-throughput detection and improving analysis efficiency; reducing the use of pumps and valves and realizing device miniaturization. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0026] Figure 1 It is the front view of the present invention;

[0027] Figure 2 It is the actual effect diagram of the present invention;

[0028] In the figure: 1-injection capillary, 2-central channel, 3-waste end capillary, 4-radial diversion channel. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] Embodiment 1:

[0031] This embodiment discloses a multi-channel microfluidic chip for parallel injection, comprising: a substrate, at least two independent radial flow-dividing channels 4 formed on the substrate, an injection capillary 1, and a central channel 2;

[0032] The radial flow distribution channel 4 and the injection capillary 1 are both connected to the side wall channel of the central channel 2;

[0033] Injection capillary 1, used for inputting samples;

[0034] The radial flow channel 4 is used for simultaneously introducing the same solution and radiates from the central channel 2 to the edge;

[0035] The central channel 2 is used to store samples so that the samples are subjected to the same force in each radial flow channel during centrifugation.

[0036] The whole chip is sealed by thermal bonding. Specifically, in this embodiment, the whole chip is sealed by epoxy resin glue.

[0037] The central channel 2 is a 1 / 4 cylinder equally divided by the center of the bottom surface of the cylinder;

[0038] The radial flow-dividing channel 4 is connected to the side wall channel on one side of the semicircular shape of the central channel 2 .

[0039] The waste liquid end capillary 3 is also included; the waste liquid end capillary 3 is used to collect the reaction waste liquid and discharge it.

[0040] The two waste liquid end capillaries 3 are adjacent to the injection capillary 1 and are arranged symmetrically with the injection capillary 1 as the axis. In this embodiment, the angle between the waste liquid end capillary 3 and the injection capillary 1 is 30 degrees.

[0041] The connections between the injection capillary 1 and the waste liquid end capillary 3, the waste liquid end capillary 3 and the radial flow splitting channel 4, and the radial flow splitting channels 4 and the radial flow splitting channels 4 are all designed with an arc structure.

[0042] The radial flow diversion channel 4 is opened according to experimental requirements.

[0043] In actual application, the syringe of the injection pump is filled with the first sample solution and pushed into the injection capillary 1. After the radial diversion channel 4 is filled with the solution, the injection pump is switched to be filled with the second sample solution and pushed into the injection capillary. The radial diversion channel 4 is filled based on the effect of pressure. The overall device of this embodiment is placed on a microfluidic chip carrier to carry out a centrifugation experiment.

[0044] Embodiment 2:

[0045] This embodiment discloses a multi-channel microfluidic chip for parallel injection based on the embodiment 1, comprising: 5 independent radial flow distribution channels 4, an injection capillary 1, and a central channel 2;

[0046] The five radial flow distribution channels 4 and the injection capillary 1 are all connected to the side wall channels of the central channel 2.

[0047] Specific:

[0048] In this embodiment, the entire chip is sealed by thermal bonding.

[0049] Among them, the central channel 2 is a cylindrical structure;

[0050] The radial branch channel 4 is connected to the side wall channel of the central channel 2 .

[0051] Among them, it also includes: a waste liquid end capillary 3;

[0052] The two waste liquid end capillaries 3 are adjacent to the injection capillary 1 and are arranged symmetrically with the injection capillary 1 as the axis. In this embodiment, the angle between the waste liquid end capillary 3 and the injection capillary 1 is 90 degrees.

[0053] The radial diversion channel 4 is 50 μm deep, 70 μm wide and 1 mm long; the waste liquid end capillary 4 has an inner diameter of 200 μm, an outer diameter of 360 μm and a length of 20 cm; the injection capillary 1 has an inner diameter of 200 μm, an outer diameter of 360 μm and a length of 20 cm; the semicircle diameter of the central channel 2 is 0.27 mm and the depth is 50 μm.

[0054] In actual operation, the syringe of the syringe pump is filled with ethyl acetate solution and the solution is pushed into the injection capillary 1 at 5 μL / min. After the radial flow channel 4 is filled with the solution, the syringe pump is switched to a syringe filled with sodium fluorescein solution and the solution is pushed into the radial flow channel 4 at 2 μL / min by the force of the injection capillary 1. It should be noted that in actual use, the sample solution is not limited to ethyl acetate solution and sodium fluorescein solution.

[0055] like Figure 2 As shown, the microfluidic chip is observed under a fluorescence microscope to have five radial flow-dividing channels 4 with equal injection volumes and flow rates, and each radial flow-dividing channel 4 can flow out the same sample at the same time.

[0056] For the device disclosed in the embodiment, since it corresponds to the content disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the partial description.

[0057] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A multi-channel microfluidic chip for parallel injection, characterized in that: include: A substrate, on which at least two independent radial flow-dividing channels (4), a sampling capillary (1), and a central channel (2) are provided; The radial flow dividing channel (4) and the injection capillary (1) are both connected to the side wall channel of the central channel (2); The injection capillary (1) is used to input a sample; The radial flow-dividing channel (4) is used for simultaneously introducing the same solution and radiates from the central channel (2) to the edge; The central channel (2) is used to store samples so that the samples are subjected to the same force in each of the radial flow channels (4) during centrifugation; The whole chip is sealed by thermal bonding; The central channel (2) is a semicircular column structure; The radial flow-dividing channel (4) is connected to the side wall channel on one side of the semicircular shape of the central channel (2); It also includes: a waste liquid end capillary (3); The plurality of waste liquid end capillaries (3) are adjacent to the injection capillary (1) and are symmetrically arranged with the injection capillary (1) as an axis; The waste liquid end capillary (3) is used to collect and discharge the reaction waste liquid; The connection between the injection capillary (1) and the waste liquid end capillary (3), the waste liquid end capillary (3) and the radial flow diversion channel (4), and the radial flow diversion channel (4) and the radial flow diversion channel (4) are all designed with an arc structure; The radial flow diversion channel (4) is opened according to experimental requirements; The radial diversion channel (4) is 50 μm deep, 70 μm wide and 1 mm long; the waste liquid end capillary (3) has an inner diameter of 200 μm, an outer diameter of 360 μm and a length of 20 cm; the injection capillary (1) has an inner diameter of 200 μm, an outer diameter of 360 μm and a length of 20 cm; the semicircular diameter of the central channel (2) is 0.27 mm and the depth is 50 μm.

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