A constant temperature system for preparing single cell nucleus suspension

Abstract

The invention discloses a constant temperature system for preparing a single cell nuclear suspension, comprising a tool rack and a constant temperature operating table, wherein the tool rack comprises a base, a liquid collection tray, a support plate, a grinding pestle hook, a scissors hook and a buckle; the constant temperature operating table comprises a display temperature control component and a constant temperature tube rack; the invention of the constant temperature system for preparing a single cell nuclear suspension fills the gap in the field related to the single cell sequencing technology, and the constant temperature system for preparing a single cell nuclear suspension has the advantages of simple operation, low price, meeting the experimental operation requirements of single cell suspension preparation, and not easily causing experimental sample contamination.

Description

Technical Field

[0001] The invention relates to the technical field of biological experiments, and in particular to a constant temperature system for preparing a single cell nucleus suspension. Background Art

[0002] Single-cell sequencing technology is an emerging technology in recent years. Many supporting technologies and equipment are still in the research and development stage. Therefore, in the process of conducting single-cell sequencing related experiments, it is always subject to various restrictions. Among them, the preparation of single-cell nuclear samples is an extremely critical process. In the preparation of nuclear suspension, the whole process needs to be in a low temperature state, so as to reduce the quenching of the nucleus and improve the quality of the nucleus.

[0003] Literature search results and market surveys show that existing related equipment is only designed for the cryopreservation of a single sample and is relatively expensive. When using existing equipment to prepare single-cell nuclear suspensions, the design does not meet the experimental operation requirements, resulting in extremely inconvenient operation. At the same time, if suspensions of multiple samples are prepared, mutual contamination is likely to occur.

[0004] Therefore, there is an urgent need for a constant temperature system for preparing single cell nucleus suspension to solve the above problems. Summary of the invention

[0005] The purpose of the present invention is to provide a constant temperature system for preparing single cell nuclear suspension, so as to solve the problem that the existing related equipment is only designed for low temperature preservation of a single sample and is relatively expensive; when using the existing equipment for preparing single cell nuclear suspension, the design does not meet the experimental operation requirements, resulting in inconvenience in operation. At the same time, if suspension preparation of multiple samples is carried out, it is easy to cause mutual contamination.

[0006] To achieve the above object, the present invention provides the following solutions:

[0007] The present invention provides a constant temperature system for preparing a single cell nucleus suspension, comprising a tool rack and a constant temperature operating table, wherein the tool rack and the constant temperature operating table are used independently or in combination;

[0008] The tool rack comprises a base, a liquid collecting tray, a support plate, a grinding pestle hook and a scissors hook, wherein the liquid collecting tray is placed on the upper surface of the base, the support plate is vertically connected to the side of the base, the grinding pestle hook is detachably fixed to the crossbeam of the support plate, and the scissors hook is fixed and embedded in the crossbeam of the support plate by a buckle;

[0009] The constant temperature operating table includes a temperature control component and a constant temperature pipe rack. The temperature control component includes a metal shell, a control module, a constant temperature base, a temperature detection module, a heating module and a refrigeration module; the control module, the temperature detection module, the heating module and the refrigeration module are all located in the metal shell, the constant temperature base is arranged on the upper surface of the metal shell, the constant temperature pipe rack is limited in the constant temperature base, the temperature detection module, the heating module and the refrigeration module are all located below the constant temperature base and are electrically connected to the control module, and the control module is externally connected to a power supply.

[0010] Preferably, the base, the support plate, the grinding pestle hook, the scissors hook and the buckle are all made of one of acrylic, polypropylene, polyethylene or polytetrafluoroethylene materials.

[0011] Preferably, the liquid collecting tray is made of stainless steel with a thickness of 0.1-0.3 mm.

[0012] Preferably, the metal shell is made of 304 stainless steel with a thickness of 0.15-0.4 mm, and an exhaust port is provided at the bottom thereof, and the exhaust port is an array of circular holes, an array of rectangular holes, or an array of square holes; a power switch is provided on the left side of the metal shell, and the power switch is electrically connected to the control module, the temperature detection module, the heating module, and the refrigeration module respectively;

[0013] The control module includes a single-chip microcomputer and an LCD display screen electrically connected thereto, wherein the single-chip microcomputer is one of a C8051 series single-chip microcomputer, a PIC series single-chip microcomputer, an MSP430F series single-chip microcomputer or an AVR series single-chip microcomputer, and the LCD display screen is arranged on the upper surface of the metal shell;

[0014] The constant temperature base includes a constant temperature base I, a constant temperature base II, a constant temperature base III, a constant temperature base IV, a constant temperature base V, a constant temperature base VI and a constant temperature base VII;

[0015] The temperature detection module includes a temperature detector and an A / D converter. The temperature detector is a thermocouple, a thermal resistor, a radiation thermometer or a non-contact infrared thermometer. The temperature detector is located below the constant temperature base and is electrically connected to the single chip computer through the A / D converter.

[0016] The heating module includes a relay I and a heating element, wherein the heating element is made of one or a mixture of nickel-chromium wire, iron-chromium-aluminum wire, PTC heating element, silicon carbide or electric heating paint plate, and the heating element is located below the constant temperature base and is electrically connected to the single-chip computer through the relay I;

[0017] The refrigeration module includes a relay II, a semiconductor refrigeration plate and an exhaust fan. The semiconductor refrigeration plate is located below the constant temperature base. The exhaust fan corresponds to the exhaust port position, and both are electrically connected to the single-chip computer through the relay II.

[0018] Preferably, the temperature range of the constant temperature operating table is 1-37°C.

[0019] Preferably, the constant temperature base and the constant temperature pipe rack are both made of one of aluminum alloy and red copper.

[0020] Preferably, the thermostatic tube rack comprises a 300 microliter PCR thermostatic tube rack, a 1.5 / 2 ml centrifuge tube thermostatic tube rack, a 15 ml centrifuge tube thermostatic tube rack, a 50 ml centrifuge tube thermostatic tube rack and a grinding pestle thermostatic tube rack.

[0021] Preferably, the 1.5 / 2ml centrifuge tube constant temperature tube rack is limited to the constant temperature base I and the constant temperature base VI, the grinding pestle constant temperature tube rack is limited to the constant temperature base II, the 15ml centrifuge tube constant temperature tube rack is limited to the constant temperature base III and the constant temperature base V, the centrifuge tube constant temperature tube rack is limited to the constant temperature base IV, and the 300 microliter PCR constant temperature tube rack is limited to the constant temperature base VII.

[0022] Compared with the prior art, the present invention has achieved the following beneficial technical effects:

[0023] 1. In the constant temperature system for preparing single cell nucleus suspension provided by the present invention, the components used are all conventional components, the manufacturing cost is low, and the constant temperature effect is excellent.

[0024] 2. The constant temperature system for preparing single cell nuclear suspension provided by the present invention can accurately maintain a constant temperature state, greatly reduce the impact of temperature changes during the preparation of single cell nuclear suspension, improve the quality of single cell suspension, and improve preparation efficiency.

[0025] 3. In the constant temperature system for preparing single cell nuclear suspension provided by the present invention, the gaps between components are reasonably designed according to the operational requirements of the single cell preparation process, which facilitates operation and greatly reduces the risk of sample contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0027] Figure 1A schematic diagram of the tool rack structure in the constant temperature system for preparing a single cell nucleus suspension provided by the present invention;

[0028] Figure 2 A schematic diagram of the upper surface structure of a constant temperature operating table in a constant temperature system for preparing a single cell nucleus suspension provided by the present invention;

[0029] Figure 3 A schematic diagram of the lower surface structure of a constant temperature operating table in a constant temperature system for preparing a single cell nucleus suspension provided by the present invention;

[0030] Figure 4 A schematic diagram of the operating principle of a constant temperature operating table in a constant temperature system for preparing a single cell nucleus suspension provided by the present invention;

[0031] In the figure: 1-base, 2-liquid collection tray, 3-support frame, 4-grinding pestle hook, 5-scissors hook, 6-buckle, 71-metal shell, 73-constant temperature base, 711-exhaust vent, 712-power switch, 721-single chip microcomputer, 722-LCD display screen, 741-temperature detector, 742-A / D converter, 751-relay I, 752-heating element, 761-relay II, 762-semiconductor refrigeration sheet, 763-exhaust fan, 81-300 microliter PCR tube constant temperature tube rack, 82-1.5 / 2ml centrifuge tube constant temperature tube rack, 83-15ml centrifuge tube constant temperature tube rack, 84-50ml centrifuge tube constant temperature tube rack, 85-grinding pestle constant temperature tube rack. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] The purpose of the present invention is to provide a constant temperature system for preparing single cell nuclear suspension, so as to solve the problem that the existing related equipment is only designed for low temperature preservation of a single sample and is relatively expensive; when using the existing equipment for preparing single cell nuclear suspension, the design does not meet the experimental operation requirements, resulting in inconvenience in operation. At the same time, if suspension preparation of multiple samples is carried out, it is easy to cause mutual contamination.

[0034] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0035] Embodiment 1:

[0036] This embodiment provides a constant temperature system for preparing a single cell nucleus suspension, such as Figure 1-4 As shown, it includes a tool rack and a constant temperature operating table. The tool rack and the constant temperature operating table are not connected, and the two can be used independently or in combination.

[0037] Specifically, the tool rack consists of a base 1, a liquid collection tray 2, a support plate 3, a grinding pestle hook 4, a scissors hook 5 and a buckle 6. The base 1, the support plate 3, the grinding pestle hook 4, the scissors hook 5 and the buckle 6 are made of acrylic sold under the trade name of acrylic board by Yunnan Corey Acrylic Products Co., Ltd. The support plate 3 is connected to the base 1 through a mortise and tenon structure, and the mortise and tenon interface is fixed with super glue; the grinding pestle hook 4 is hung on the crossbeam of the support plate 3 and connected using a mortise and tenon structure; the scissors hook 5 is embedded in the crossbeam of the support plate 3 and fixed with a buckle 6.

[0038] The base 1 is used to stably support the entire tool rack; the main purpose of the support plate 3 is to bear the weight of the hung instruments, and is used to carry the grinding pestle hook 4 and the scissors hook 5; the grinding pestle hook 4 is used to place the grinding pestle, which can effectively prevent the grinding pestle from slipping and breaking, and at the same time prevent the grinding pestle from shaking left and right, contacting each other, and causing mutual contamination; the scissors hook 5 is used to place the scissors used in the experiment, to prevent the scissors from slipping and causing contamination and personal injury.

[0039] The liquid collecting tray 2 is made of 304 stainless steel sold by Boyuan Stainless Steel Co., Ltd. under the trade name of 304 stainless steel plate, with a thickness of 0.15mm; the liquid collecting tray 2 is placed above the base 1; the purpose of the liquid collecting tray 2 is to collect the liquid dripping from the utensil to prevent it from dripping onto the operating table and causing sample contamination; the thickness of the 304 stainless steel used to prepare the liquid collecting tray 2 is 0.1-0.3mm. If the thickness is less than 0.1mm, the preparation difficulty coefficient is high and it is not easy to prepare and form. If the thickness is greater than 0.3mm, the prepared liquid collecting tray 2 is too heavy and difficult to move, and the preparation cost is increased. Therefore, the appropriate thickness of 304 stainless steel is 0.1-0.3mm, and preferably 0.15-0.25mm

[0040] Furthermore, the constant temperature operating table includes a temperature control component 7 and a constant temperature pipe rack 8; the temperature control component 7 includes a metal shell 71, a control module 72, a constant temperature base 73, a temperature detection module 74, a heating module 75 and a refrigeration module 76; the constant temperature base 73 is made of an aluminum alloy sold by Jiangsu Huanhai Metal Products Co., Ltd. under the trade name of aluminum alloy plate 1060; the constant temperature pipe rack 8 is made of an aluminum alloy sold by Jiangsu Huanhai Metal Products Co., Ltd. under the trade name of aluminum alloy plate 1060, both of which have excellent electrical conductivity, thermal conductivity and corrosion resistance. The constant temperature base 73 and the constant temperature pipe rack 8 utilize their excellent thermal conductivity to keep the reagents or samples within the set temperature range at all times, thereby enhancing the experimental effect.

[0041] The operating principle of the constant temperature operating table is as follows: first, the target temperature of the constant temperature base 73 is set in the control module 72, the real-time temperature of the constant temperature base 73 is measured by the temperature detection module 74, and the feedback is sent to the control module 72. After the control module 72 compares and analyzes the real-time temperature with the target temperature, it sends a heating / cooling instruction to the heating module 75 / cooling module 76. The heating module 75 / cooling module 76 executes the instruction to heat / lower the temperature of the constant temperature base 73, and maintain the constant temperature after reaching the target temperature.

[0042] The temperature range of the constant temperature operating table B is 1-37°C. If the temperature is less than 1, it is easy to cause the pre-cooled reagent to freeze, which is not conducive to the experiment. If the temperature is greater than 37°C, the quenching of the cell nucleus is accelerated, resulting in a sharp decrease in the amount of cell nuclei, and it may also cause burns to the experimenter. Therefore, the temperature of the constant temperature operating table B is suitable for 1-37°C, preferably 4-8°C. The temperature range of the constant temperature operating table in this embodiment is 2-36°C; the exhaust port 711 below the metal shell 71 is an array of circular holes, and its main function is to serve as a channel for the equipment to exchange internal and external heat. There is a power switch 712 on the left side of the metal shell 71. The function of the power switch 712 is to turn on and off the power supply, and protect the equipment from short circuits, overloads, etc. The metal shell 71 is made of 304 stainless steel sold by Boyuan Stainless Steel Co., Ltd. under the trade name of 304 stainless steel plate, with a thickness of 0.25mm. The power switch 712 is a power switch sold by Shenzhen Jicheng Technology Co., Ltd. under the trade name KCD11 ship-shaped power switch.

[0043] The control module 72 includes a C8051 series microcontroller 721 sold by Shenzhen Baisihao Electronic Technology Co., Ltd. under the trade name C8051F410-GQR and an LCD display screen 722 sold by Guangzhou Chihong Electronic Technology Manufacturing Co., Ltd. under the trade name Embedded Resistive Touch Screen.

[0044] The function of the single chip computer 721 is to receive the temperature information fed back by the temperature detector 741, calculate and analyze the received information, and send the next execution instruction. The function of the LCD display screen 722 is to display the implementation temperature, the preset target temperature and control the heating / cooling area. It has the advantages of thin body, space saving, low energy consumption, no heat and no radiation, and is good for health.

[0045] The temperature detection module 74 includes a thermocouple 741 sold by Taizhou Wanyang Automation Equipment Co., Ltd. under the trade name of Thermocouple Platinum Temperature Probe WY-WZP and an A / D converter 742 sold by Shenzhen Jinhong Century Technology Co., Ltd. under the trade name of A / D Converter ES7243.

[0046] The function of the temperature detector 741 is to detect the real-time temperature of the constant temperature base 73 and transmit it to the single-chip computer 721 through the A / D converter 742. The function of the A / D converter 742 is to convert the analog quantity that is continuous in time and amplitude into a digital signal that is discrete in time and amplitude, that is, a circuit that converts an analog signal into a digital signal.

[0047] The heating module 75 includes a relay I751 sold by Yuanhuang Electric Technology under the trade name of small relay HH52P and a nickel-chromium wire heating element 752 sold by Shuntai Electric Heating Materials Co., Ltd. under the trade name of nickel-chromium wire cr20ni80.

[0048] Refrigeration module 76 is composed of relay II 761 sold by Yuanhuang Electric Technology under the trade name small relay HH52P, semiconductor cooling plate 762 sold by Shenzhen Youxin Electronic Technology Co., Ltd. under the trade name TEC2-19006 double-layer semiconductor cooling plate, and exhaust fan 763 sold by Changzhou Taiming Electronic Appliance under the trade name small axial flow fan.

[0049] The function of relay I 751 is to control the heating device with the contacts of the relay, and the function of relay II 761 is to control the refrigeration device and the ventilation device with its contacts; the function of the heating element 752 is to directly convert electrical energy into thermal energy through the principle of Joule heating, so that the temperature of the equipment rises to the set temperature; the semiconductor refrigeration plate 762 utilizes the Peltier effect of semiconductor materials. When direct current passes through a thermocouple formed by two different semiconductor materials in series, heat can be absorbed and released at both ends of the thermocouple respectively, so as to achieve the purpose of refrigeration; its advantage is that there are no sliding parts, and it is used in some occasions where space is limited, reliability is high, and there is no refrigerant pollution; its function is to quickly reduce the temperature of the equipment to the set temperature; the function of the exhaust fan 763 is mainly to dissipate heat for the hot end of the semiconductor refrigeration plate 762. Usually, the temperature difference between the hot and cold ends of the semiconductor refrigeration plate 762 can reach between 40 and 65°C. The exhaust fan 763 actively discharges the heat generated by the hot end through the exhaust port 711, so that the temperature of the hot end of the semiconductor refrigeration plate 762 is reduced, and the temperature of the cold end is reduced accordingly, thereby reaching a lower temperature.

[0050] The control module 72 , the constant temperature base 73 , the temperature detection module 74 , the heating module 75 , the cooling module 76 and the power switch 712 are electrically connected; the constant temperature pipe rack 8 is placed above the constant temperature base 73 .

[0051] The matching relationship between the constant temperature base 73 and the constant temperature tube rack 82 is: a 1.5 / 2 ml centrifuge tube constant temperature tube rack 82 is placed on the constant temperature base I 731 and the constant temperature base VI 736, a constant temperature tube rack 85 is placed on the constant temperature base II 732 grinding pestle, a 15 ml centrifuge tube constant temperature tube rack 83 is placed on the constant temperature base III 733 and the constant temperature base V 735, a 50 ml centrifuge tube constant temperature tube rack 84 is placed on the constant temperature base IV 734, and a 300 microliter PCR constant temperature tube rack 81 is placed on the constant temperature base VII 737.

[0052] Embodiment 2:

[0053] The implementation method of this embodiment is the same as that of Example 1, except that the base 1, liquid collection tray 2, support plate 3, grinding pestle hook 4, scissors hook 5 and buckle 6 are made of polypropylene sold by Xinxinhong Plastic Co., Ltd. under the trade name of polypropylene PP plate, and the 304 stainless steel used to prepare the liquid collection tray 2 has a thickness of 0.2 mm.

[0054] The constant temperature base 73 is made of copper sold by Dezhou Hengsheng Nonferrous Metals Co., Ltd. under the trade name T2 copper plate.

[0055] The temperature range of the constant temperature operating table is 4-30°C; the exhaust port 711 below the metal shell 71 is an array of square holes, and the thickness of the stainless steel used to prepare the metal shell 71304 is 0.28mm.

[0056] The control module 72 includes a MSP430F series microcontroller 721 sold by Ningbo Xinchuang Electronic Technology under the trade name MSP430F149 and an LCD display screen 722 sold by Shenzhen Hengyuwei LCD Screen under the trade name TFT Liquid Crystal Capacitive Touch Screen HYW070E01.

[0057] The temperature detection module 74 includes a thermal resistor 741 sold by Shanghai Yuhao Electric Heating Appliance Co., Ltd. under the trade name Thermal Resistor WZP-187 Waterproof Temperature Probe.

[0058] The heating module 75 includes a relay Ⅰ751 sold by Shanghai Jinke Electric Automation under the trade name relay MY2N-GS and an iron-chromium-aluminum wire heating element 752 sold by Shuntai Electric Heating Materials Co., Ltd. under the trade name iron-chromium-aluminum wire ocr27al17mo2.

[0059] Refrigeration module 76 is composed of relay II 761 sold by Shanghai Jinke Electric Automation under the trade name relay MY2N-GS, high-end semiconductor cooling plate 762 sold by Shenzhen Xinhengjie Electronic Technology under the trade name XH-C1206, and exhaust fan 763 sold by Foshan Qianmeng Electric Co., Ltd. under the trade name louver exhaust fan.

[0060] Embodiment 3:

[0061] The implementation method of this embodiment is the same as that of Example 1, except that the base 1, liquid collection tray 2, support plate 3, grinding pestle hook 4, scissors hook 5 and buckle 6 are made of polyethylene sold under the trade name of ultra-high molecular polyethylene plate by Shandong Huatuo Rubber and Plastic Products Co., Ltd., and the 304 stainless steel used to prepare the liquid collection tray 2 has a thickness of 0.25 mm.

[0062] The constant temperature pipe rack 8 is made of red copper sold by Dezhou Hengsheng Nonferrous Metals Co., Ltd. under the trade name T2 red copper plate.

[0063] The temperature range of the constant temperature operating table is 8-20°C; the exhaust port 711 below the metal shell 71 is an array of square holes, and the thickness of the stainless steel used to prepare the metal shell 71304 is 0.29mm.

[0064] The control module 72 includes an AVR series microcontroller 721 sold by Shenzhen Landray Technology Co., Ltd. under the trade name AVR microcontroller MCU and an LCD display screen 722 sold by Shenzhen Hengyuwei LCD Screen under the trade name TFT liquid crystal capacitive touch screen HYW070E01.

[0065] The temperature detection module 74 includes an infrared thermometer 741 sold by Nanjing Shunlaida Measurement and Control Equipment Co., Ltd. under the trade name LSCI-DT100 Infrared Thermometer.

[0066] The heating module 75 includes a relay I751 sold by Yueqing Qinghe Electric under the trade name of relay JQX-60F and a PTC heating element 752 sold by Jiangsu Senxin Thermal Instrument Equipment Manufacturing Co., Ltd. under the trade name of PTC ceramic heater.

[0067] The cooling module 76 includes a relay II 761 sold by Yueqing Qinghe Electric under the trade name of relay JQX-60F, a high-end semiconductor cooling sheet 762 sold by Shenzhen Xinhengjie Electronic Technology under the trade name of XH-C1206, and an exhaust fan 763 sold by Foshan Qianmeng Electric Co., Ltd. under the trade name of louver exhaust fan.

[0068] The present invention uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only used to help understand the method and core ideas of the present invention. At the same time, for those skilled in the art, according to the ideas of the present invention, there will be changes in the specific implementation methods and application scopes. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A constant temperature system for preparing a single cell nucleus suspension, characterized in that: It includes a tool rack and a constant temperature operating table, and the tool rack and the constant temperature operating table are used independently or in combination; The tool rack comprises a base (1), a liquid collecting tray (2), a support plate (3), a grinding pestle hook (4) and a scissors hook (5); the liquid collecting tray (2) is placed on the upper surface of the base (1); the support plate (3) is vertically connected to the side of the base (1); the grinding pestle hook (4) is detachably fixed to the crossbeam of the support plate (3); and the scissors hook (5) is fixedly embedded in the crossbeam of the support plate (3) by a buckle (6); The constant temperature operating table comprises a temperature control component (7) and a constant temperature pipe rack (8); the temperature control component (7) comprises a metal shell (71), a control module (72), a constant temperature base (73), a temperature detection module (74), a heating module (75) and a refrigeration module (76); the control module (72), the temperature detection module (74), the heating module (75) and the refrigeration module (76) are all located in the metal shell (71); the constant temperature base (73) is arranged on the upper surface of the metal shell (71); the constant temperature pipe rack (8) is limited in the constant temperature base (73); the temperature detection module (74), the heating module (75) and the refrigeration module (76) are all located below the constant temperature base (73) and are electrically connected to the control module (72); the control module (72) is externally connected to a power supply; The metal shell (71) is made of 304 stainless steel with a thickness of 0.15-0.4 mm. An exhaust port (711) is provided at the bottom thereof. The exhaust port (711) is an array of circular holes, an array of rectangular holes or an array of square holes. A power switch (712) is provided on the left side of the metal shell (71). The power switch is electrically connected to the control module (72), the temperature detection module (74), the heating module (75) and the cooling module (76) respectively. The control module (72) comprises a single-chip microcomputer (721) and an LCD display screen (722) electrically connected thereto, the single-chip microcomputer (721) being one of a C8051 series single-chip microcomputer, a PIC series single-chip microcomputer, an MSP430F series single-chip microcomputer or an AVR series single-chip microcomputer, and the LCD display screen (722) is arranged on the upper surface of the metal housing (71); The constant temperature base (73) includes a constant temperature base I (731), a constant temperature base II (732), a constant temperature base III (733), a constant temperature base IV (734), a constant temperature base V (735), a constant temperature base VI (736) and a constant temperature base VII (737); The temperature detection module (74) comprises a temperature detector (741) and an A / D converter (742); the temperature detector (741) is a thermocouple, a thermal resistor, a radiation thermometer or a non-contact infrared thermometer; the temperature detector (741) is located below the constant temperature base (73) and is electrically connected to the single chip computer (721) via the A / D converter (742); The heating module (75) comprises a relay I (751) and a heating element (752), wherein the heating element (752) is made of one or a mixture of nickel-chromium wire, iron-chromium-aluminum wire, PTC heating element, silicon carbide or electric heating coating plate, and the heating element (752) is located below the constant temperature base (73) and is electrically connected to the single-chip computer (721) through the relay I (751); The refrigeration module (76) comprises a relay II (761), a semiconductor refrigeration plate (762) and an exhaust fan (763), wherein the semiconductor refrigeration plate (762) is located below the constant temperature base (73), the exhaust fan (763) corresponds to the position of the exhaust port (711), and both are electrically connected to the single chip computer (721) via the relay II (761).

2. The constant temperature system for preparing a single cell nucleus suspension according to claim 1, characterized in that: The base (1), the support plate (3), the grinding pestle hook (4), the scissors hook (5) and the buckle (6) are all made of one of acrylic, polypropylene, polyethylene or polytetrafluoroethylene materials.

3. The constant temperature system for preparing a single cell nucleus suspension according to claim 1, characterized in that: The liquid collecting tray (2) is made of stainless steel and has a thickness of 0.1-0.3 mm.

4. The constant temperature system for preparing a single cell nucleus suspension according to claim 1, characterized in that: The temperature range of the constant temperature operating table is 1-37°C.

5. The constant temperature system for preparing a single cell nucleus suspension according to claim 1, characterized in that: The constant temperature base (73) and the constant temperature pipe rack (8) are both made of one of aluminum alloy and red copper.

6. The constant temperature system for preparing a single cell nucleus suspension according to claim 1, characterized in that: The thermostatic tube rack (8) comprises a 300 microliter PCR thermostatic tube rack (81), a 1.5 / 2 ml centrifuge tube thermostatic tube rack (82), a 15 ml centrifuge tube thermostatic tube rack (83), a 50 ml centrifuge tube thermostatic tube rack (84) and a grinding pestle thermostatic tube rack (85).

7. The constant temperature system for preparing a single cell nucleus suspension according to claim 6, characterized in that: The 1.5 / 2 ml centrifuge tube thermostatic tube rack (82) is located on the thermostatic base I (731) and the thermostatic base VI (736), the grinding pestle thermostatic tube rack (85) is located on the thermostatic base II (732), and the 15 ml centrifuge tube thermostatic tube rack (83) is located on the thermostatic base III (733) and the thermostatic base V. (735), the centrifuge tube constant temperature tube rack (84) is limited to the constant temperature base IV (734), and the 300 microliter PCR constant temperature tube rack (81) is limited to the constant temperature base VII (737).

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