A method for interplanting Artemisia annua and tea tree

Abstract

The invention discloses an interplanting method of Artemisia annua and tea trees, which belongs to the technical field of interplanting of crops, and includes the steps of site selection and land preparation, Artemisia annua planting, interplanting of tea trees, and planting and maintenance. The invention also discloses a novel protective film for solving the problem of insect pests when interplanting Artemisia annua and tea trees, so that Artemisia annua and tea trees are not prone to root rot when interplanted, and at the same time provides an interplanting method for long-term pest removal, so that diseases are reduced during the growth of Artemisia annua and tea trees, so that crops grow well and economic benefits are increased.

Description

Technical Field

[0001] The invention relates to the technical field of interplanting crops, and in particular to an interplanting method of Artemisia annua and tea trees. Background Art

[0002] Artemisia annua is an annual herb, belonging to the genus Artemisia annua of the Asteraceae family. The plant height is more than 40-150cm, the whole growth period is about 120 days, it likes moisture, avoids drought, is afraid of waterlogging, and requires sufficient light. Artemisia annua is rough and easy to manage, has a short growth period, low investment, and fast returns. Artemisia annua has high medicinal value, and the main active ingredient in Artemisia annua is artemisinin and its derivatives. Artemisinin is mainly used to treat malaria, tuberculosis hot flashes, heatstroke, skin itching, urticaria, seborrheic dermatitis, and mosquito control. The whole plant is used as medicine, washed and used fresh or dried for medicine, so the market demand for Artemisia annua is very large.

[0003] Tea trees are shrubs or small trees of the Theaceae family and the genus Camellia, with hairless young branches. Tea leaves can be used to make tea, and seeds can be used to extract oil. Tea trees prefer warm and humid climates. When the average temperature is above 10°C, buds begin to sprout. The optimum growth temperature is 20-25°C, and the annual precipitation must be above 1,000 mm. Tea trees are light-loving and shade-tolerant, and their life span is divided into seedling stage, juvenile stage, adult stage, and aging stage. Trees can be up to 100 to 200 years old, but their economic age is generally 40 to 50 years.

[0004] There are currently no cases of interplanting of two crops on the market. Underground pests often occur in tea gardens, and tea trees will face a variety of diseases during the planting process, such as cutworms, powdery mildew, and damping-off. Cutworms will lurk in the soil and feed on the rhizomes of tea seedlings and Artemisia annua, often biting off the seedlings. In severe cases, a large number of seedlings will die due to root system breaks. At the same time, Artemisia annua is relatively less susceptible to diseases, but it will also suffer from a small number of diseases such as powdery mildew and damping-off. Weeds are the main place for cutworms and other pests to lay eggs in early spring. Protective film covering can reduce the occurrence of weeds, keep warm and moisturize, and control weeds. Therefore, covering with protective film will reduce pests such as cutworms. The current prevention and control methods for diseases such as powdery mildew and damping-off are to apply pesticides and disinfect for prevention and control. Although crops grow fast and have luxuriant branches and leaves after covering with protective film, they are often easy to attract pests, resulting in early and serious pests, affecting the yield-increasing effect of film covering. At the same time, although the protective film has the function of retaining water, it prevents external precipitation from entering the ridge. If no measures are taken, the plants may suffer from water deficiency during the vigorous growth period, affecting normal growth; if there is continuous rainfall, it is easy to cause serious water stains, which will deteriorate the permeability of the soil and hinder water evaporation, which will also affect the growth of the plants. In addition, the traditional protective film is a non-degradable plastic, which is not strong enough and will make it difficult to recycle. If it is not recycled in time after use, it will destroy the soil structure, affect the growth of crop roots and pollute the environment.

[0005] Therefore, it is necessary to find an interplanting method that can reduce diseases of interplanted crops during their growth and make the crops grow well. Summary of the invention

[0006] In view of this, the purpose of the present invention is to provide a method for intercropping Artemisia annua and tea trees, which can solve the problems of insect pests and diseases in the intercropping process for a long time by covering with a protective film, so as to reduce diseases when intercropping Artemisia annua and tea trees, make the crops grow well and increase profits.

[0007] The present invention solves the above technical problems by the following technical means:

[0008] A method for interplanting Artemisia annua and tea trees, comprising the following steps:

[0009] (1) Site selection and land preparation: The soil is plowed and leveled, and then piled into soil ridges. The soil ridges are divided into two rows, and drainage ditches are dug between the ridges.

[0010] (2) Artemisia annua planting: Use 800 times carbendazim solution to spray the soil in the soil bed and apply base fertilizer. Sow Artemisia annua seeds evenly in any row of the soil bed at a sowing density of 0.3-0.8 kg per square meter and lay a protective film;

[0011] (3) Tea interplanting: After the Artemisia annua grows to 8-10 cm, select strong and uniform-sized tea seedlings of the current year, soak them in yellow mud, and then plant them in another row of the same soil bed where Artemisia annua is not sown;

[0012] (4) Planting and maintenance: water and fertilizer management for Artemisia annua and tea trees;

[0013] Preferably, after planting, if dead or diseased seedlings are found, they should be removed in time, and seedlings should be replanted on cloudy or light rainy days. After planting, weeding should be carried out according to the growth of weeds.

[0014] Preferably, when the tea seedlings grow to 10-15 cm in height, 40-60 kg of water is poured into the soil per mu. After the watering treatment is completed, the tea seedlings are topped and the branches are pruned on a sunny day every 1-2 days. After the topping and branch pruning are completed, 800 times potassium permanganate solution is used for spraying and disinfection at a dosage of 15-18 kg per mu, the soil is loosened between the rows, and base fertilizer is applied.

[0015] Furthermore, the earth ridge has a width of 0.6-0.8m and a height of 40cm; the drainage ditch has a width of 0.4-0.6m to prevent water accumulation from causing root rot of tea trees and Artemisia annua. The earth ridge is divided into two rows in a 1:1 width ratio, which can facilitate the centralized harvesting of the same crops and the continuation of subsequent interplanting.

[0016] Furthermore, the base fertilizer is mixed with the following raw materials: 50-60 parts by mass of ammonium phosphate, 80-110 parts by mass of urea, 20-30 parts by mass of dipotassium hydrogen phosphate, 70-80 parts by mass of superphosphate, 7-11 parts by mass of humic acid, 0.9-1.1 parts by mass of magnesium chloride, and 0.1-0.5 parts by mass of manganese sulfate.

[0017] Furthermore, the protective film includes the following raw materials: 3-8 parts by mass of glyceryl monostearate, 7-10 parts by mass of rice gluten, 1-2 parts by mass of polyethylene glycol monomethyl ether, 1.4-2 parts by mass of tea polyphenols, 4-5 parts by mass of corn starch, 60-80 parts by mass of modified polyvinyl alcohol, 0.5-1 parts by mass of sodium alginate, 1-1.8 parts by mass of cyclodextrin, and 0.1-0.5 parts by mass of xanthan gum.

[0018] Further, the preparation method of the protective film is as follows:

[0019] (1) Stir modified polyvinyl alcohol, polyethylene glycol monomethyl ether and tea polyphenols in a high-speed mixer at 400-500 r / min and 200-220° C. for 10-15 min to form a uniform mixed melt;

[0020] (2) Add monostearate and rice gluten to sodium alginate, add appropriate amount of water and mix well, then add cyclodextrin, xanthan gum and corn starch for coating, stir for 20-40 minutes, then add the mixed melt, stir at 170-190°C at 100-200 r / min for 20-40 minutes, and then blow film to obtain a protective film.

[0021] The modified polyvinyl alcohol of the present invention has water resistance, is melt-blended with polyethylene glycol monomethyl ether and tea polyphenols in a mixer, so that active free radicals are added to the polyvinyl alcohol skeleton, and is blended with subsequent corn starch, cyclodextrin and xanthan gum at high temperature to form a film. As the laying time increases, the corn starch is decomposed, and the permeability of the protective film is gradually increased, so that the evaporation of the soil is prevented from being blocked after laying on the ground. Sodium alginate, cyclodextrin, xanthan gum and rice glutenin are mixed to form a colloidal substance, so that the rice glutenin is prevented from being decomposed too early during the secondary melt blending modification, and cannot fully react with the modified polyvinyl alcohol, so the rice glutenin is wrapped by the colloidal substance to delay the reaction start time. After the modified polyvinyl alcohol reacts with rice gluten, the strength of the modified polyvinyl alcohol is further increased, and the modified polyvinyl alcohol has the biocompatibility of protein. At the same time, the added monostearate glyceryl has a bactericidal function. During the use of the protective film, the monostearate glyceryl can be released with rainfall and irrigation, penetrate into the soil to slowly kill bacteria and prevent pests, making it degradable and sterilizing, and not easy to be decomposed. After laying the protective film, most of the tea garden is covered by the protective film, with less exposed ground, which is not conducive to the continued laying of eggs by cutworms. At the same time, because the protective film is close to the ground, it is not conducive to the growth of weeds, reducing the food of cutworms and reducing pests. The protective film slowly releases effective bactericidal substances, which can prevent and control diseases such as powdery mildew and damping-off for a long time.

[0022] Preferably, the prepared pellets are blown into film on a film blowing unit, and the extrusion temperature is controlled at 180-220°C.

[0023] Furthermore, the modified polyvinyl alcohol comprises the following raw materials: 10-12 parts by mass of polyvinyl alcohol, 20-30 parts by mass of 3wt% sodium hydroxide solution, 5-8 parts by mass of chloroacetyl chloride, 0.3-0.5 parts by mass of N,N-diisopropylethylamine, 0.5-0.7 parts by mass of glycerol, and 3-5 parts by mass of polyamide epichlorohydrin.

[0024] Further, the preparation steps of the modified polyvinyl alcohol are as follows:

[0025] Dissolve chloroacetyl chloride and N,N-diisopropylethylamine in dimethyl sulfoxide to obtain a mixed solution for standby use;

[0026] Add 3wt% sodium hydroxide solution to polyvinyl alcohol, heat to 70-85°C after mixing, stir and react at constant temperature for 1-2h, add the mixed solution, keep the temperature for reaction for 40-50min, add propylene glycol and continue to mix and stir for 10-20min, then add polyamide epichlorohydrin, continue to stir for 30-60min, cool and dry to constant weight, grind and crush to obtain modified polyvinyl alcohol.

[0027] When preparing the protective film, the hydrophilic polyvinyl alcohol is destroyed by sodium hydroxide to destroy the hydrogen bonds between molecules and within molecules, so that the hydrogen bonds are exposed, so that it undergoes a substitution reaction with chloroacetyl chloride under the action of N, N-diisopropylethylamine, so that the hydrogen in the hydroxyl group on the polyvinyl alcohol is replaced, forming a stable and difficult-to-break staggered chain network structure, which retains the water absorption of polyvinyl alcohol while improving the strength. It is blended and modified with polyamide epichlorohydrin and propylene glycol, and the high molecular mucus is evenly blended to enhance the mechanical strength, and finally modified polyvinyl alcohol is obtained. Beneficial Effects

[0028] (1) The intercropping method of the present invention solves the problem of insect pests when intercropping Artemisia annua and tea trees, and can obtain high economic benefits in actual production.

[0029] (2) The protective film prepared by the present invention is used in the intercropping process to prevent the roots of Artemisia annua and tea trees from rotting and provide a long-term method for removing insect pests and diseases.

[0030] (3) The present invention produces a slow-release bactericidal protective film during the intercropping process. The high-strength ground film has good heat preservation, moisture retention and weed suppression effects, which can promote crop yields. It is easier to pick up and recycle after use, which can ensure the recyclability of the ground film from the source. At the same time, it also has a certain degree of degradability and is not easy to cause pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 : Schematic diagram of intercropping of Artemisia annua and tea trees;

[0032] Figure 2 : Growth diagram of Artemisia annua in experimental group 1 during intercropping. DETAILED DESCRIPTION

[0033] The present invention will be described in detail below with reference to the embodiments and accompanying drawings:

[0034] 1. Preparation of modified polyvinyl alcohol

[0035] The present invention provides a method for interplanting Artemisia annua and tea trees. However, before interplanting, a protective film and modified polyvinyl alcohol as a raw material in the protective film need to be prepared. The raw material of the modified polyvinyl alcohol is weighed according to the data in Table 1, and the specific ratio is as follows:

[0036] Table 1 (Unit: kg)

[0037]

[0038] The specific preparation steps of each embodiment and comparative example are as follows:

[0039] Example 1: Preparation of modified polyvinyl alcohol

[0040] Add chloroacetyl chloride and N,N-diisopropylethylamine to an appropriate amount of dimethyl sulfoxide for dissolution. The amount of dimethyl sulfoxide added is just enough to dissolve chloroacetyl chloride and N,N-diisopropylethylamine to obtain a mixed solution for standby use.

[0041] Add 3wt% sodium hydroxide solution to polyvinyl alcohol, mix and heat to 77°C, stir and react at 77°C for 1.5h, add the mixed solution, keep 77°C for reaction for 45min, add propylene glycol and continue to mix and stir for 15min, then add polyamide epichlorohydrin, continue to stir for 45min, cool and dry to constant weight, grind and crush to obtain modified polyvinyl alcohol.

[0042] Example 2: Preparation of modified polyvinyl alcohol

[0043] Add chloroacetyl chloride and N,N-diisopropylethylamine to an appropriate amount of dimethyl sulfoxide for dissolution. The amount of dimethyl sulfoxide added is just enough to dissolve chloroacetyl chloride and N,N-diisopropylethylamine to obtain a mixed solution for standby use.

[0044] Add 3wt% sodium hydroxide solution to polyvinyl alcohol, mix and heat to 70°C, stir and react at a constant temperature of 70°C for 2h, add the mixed solution, keep the temperature at 70°C for 45min, add propylene glycol and continue to mix and stir for 20min, then add polyamide epichlorohydrin and continue to stir for 60min, cool and dry to constant weight, grind and crush to obtain modified polyvinyl alcohol.

[0045] Example 3: Preparation of modified polyvinyl alcohol

[0046] Add chloroacetyl chloride and N,N-diisopropylethylamine to an appropriate amount of dimethyl sulfoxide for dissolution. The amount of dimethyl sulfoxide added is just enough to dissolve chloroacetyl chloride and N,N-diisopropylethylamine to obtain a mixed solution for standby use.

[0047] Add 3 wt% sodium hydroxide solution to polyvinyl alcohol, mix and heat to 85°C, stir and react at a constant temperature of 85°C for 1 hour, add the mixed solution, keep the temperature at 85°C for 45 minutes, add propylene glycol and continue to mix and stir for 10 minutes, then add polyamide epichlorohydrin and continue to stir for 45 minutes, cool and dry to constant weight, grind and crush to obtain modified polyvinyl alcohol.

[0048] Comparative Example 1: Preparation of modified polyvinyl alcohol

[0049] The steps of this comparative example are the same as those of Example 1, except that propylene glycol and chloroacetyl chloride are missing. The specific steps are as follows:

[0050] Add an appropriate amount of N,N-diisopropylethylamine to dimethyl sulfoxide for dissolution. The amount of dimethyl sulfoxide added is just enough to dissolve chloroacetyl chloride and N,N-diisopropylethylamine to obtain a mixed solution for standby use.

[0051] Add 3 wt% sodium hydroxide solution to polyvinyl alcohol, mix and heat to 77°C, stir and react at 77°C for 1.5 hours, add the mixed solution, keep the temperature at 77°C for 45 minutes, add polyamide epichlorohydrin, continue stirring for 45 minutes, cool and dry to constant weight, grind and crush to obtain modified polyvinyl alcohol.

[0052] Comparative Example 2: Preparation of modified polyvinyl alcohol

[0053] The steps of this comparative example are the same as those of Example 1, except that polyamide epichlorohydrin is missing. The specific steps are as follows:

[0054] Add chloroacetyl chloride and N,N-diisopropylethylamine to an appropriate amount of dimethyl sulfoxide for dissolution. The amount of dimethyl sulfoxide added is just enough to dissolve chloroacetyl chloride and N,N-diisopropylethylamine to obtain a mixed solution for standby use.

[0055] Add 3 wt % sodium hydroxide solution to polyvinyl alcohol, mix and heat to 77 ° C, stir and react at 77 ° C for 1.5 hours, add the mixed solution, keep 77 ° C to react for 45 minutes, add propylene glycol and continue to mix and stir for 15 minutes, cool and dry to constant weight, grind and crush to obtain modified polyvinyl alcohol.

[0056] 2. Preparation of protective film

[0057] The present invention provides a method for interplanting Artemisia annua and tea trees, but a protective film needs to be prepared before interplanting. The raw materials of the protective film prepared by the present invention are weighed according to the data in Table 2, and the specific ratio is as follows:

[0058] Table 2 (Unit: kg)

[0059]

[0060] The modified polyvinyl alcohol used in Examples 4-6 and Comparative Examples 5-7 is the modified polyvinyl alcohol prepared in Example 1, Comparative Examples 3 and 4 use the modified polyvinyl alcohol prepared in Comparative Examples 1 and 2 respectively, Comparative Example 8 does not use modified polyvinyl alcohol, and Comparative Example 9 uses ordinary unmodified polyvinyl alcohol.

[0061] The specific preparation steps are as follows:

[0062] Example 4: Preparation of protective film

[0063] (1) Stir modified polyvinyl alcohol, polyethylene glycol monomethyl ether and tea polyphenols in a high-speed mixer at 450 r / min and 220° C. for 13 min to form a uniform mixed melt;

[0064] (2) Take monostearate glyceryl, rice gluten and add sodium alginate, mix with 10 kg of water and stir, then add cyclodextrin, xanthan gum and corn starch for coating, stir for 30 minutes and then add the mixed melt, stir at 180°C at 150 r / min for 30 minutes in a mixer and then blow film extrude at 200°C to obtain a protective film with a thickness of 0.015 mm.

[0065] Example 5: Preparation of protective film II

[0066] (1) Stir modified polyvinyl alcohol, polyethylene glycol monomethyl ether and tea polyphenols in a high-speed mixer at 400 r / min and 220° C. for 15 min to form a uniform mixed melt;

[0067] (2) Take monostearate glyceryl, rice gluten and sodium alginate, add them to 10 kg of water, stir them, add cyclodextrin, xanthan gum and corn starch to wrap, stir for 30 minutes, then add the mixed melt, stir at 180°C at 100 r / min for 30 minutes, and then blow film extrude at 180°C to obtain a protective film with a thickness of 0.015 mm.

[0068] Example 6: Preparation of protective film III

[0069] (1) Stir modified polyvinyl alcohol, polyethylene glycol monomethyl ether and tea polyphenols in a high-speed mixer at 500 r / min and 220° C. for 10 min to form a uniform mixed melt;

[0070] (2) Take monostearate glyceryl, rice gluten, add sodium alginate and 10 kg of water, add stirring, then add cyclodextrin, xanthan gum and corn starch for coating, stir for 30 minutes, then add the mixed melt, stir at 180°C at 200 r / min for 30 minutes, and then blow film extrude at 220°C to obtain a protective film with a thickness of 0.015 mm.

[0071] Comparative Example 3: Preparation of protective film

[0072] The steps of this comparative example are the same as those of Example 4, except that the modified polyvinyl alcohol prepared in Example 1 used in step (1) of Example 4 is replaced by the modified polyvinyl alcohol prepared in Comparative Example 1.

[0073] Comparative Example 4: Preparation of protective film

[0074] The steps of this comparative example are the same as those of Example 4, except that the modified polyvinyl alcohol prepared in Example 1 used in step (1) of Example 4 is replaced by the modified polyvinyl alcohol prepared in Comparative Example 2.

[0075] Comparative Example 5: Preparation of protective film

[0076] The steps of this comparative example are the same as those of Example 4, except that the raw materials used in step (2) lack rice gluten and glyceryl monostearate, and step (1) is the same as that of Example 1. Specifically, the process of step (2) is as follows:

[0077] (2) Sodium alginate was mixed with 10 kg of water and then added with stirring. Cyclodextrin, xanthan gum and corn starch were added for coating. The mixture was stirred for 30 min and then added into the mixed melt. The mixture was stirred at 150 r / min for 30 min at 180 °C and then blown into film for extrusion. The extrusion temperature was 200 °C to obtain a protective film with a thickness of 0.015 mm.

[0078] Comparative Example 6: Preparation of protective film

[0079] The steps of this comparative example are the same as those of Example 4, except that the raw material used in step 1 lacks polyethylene glycol monomethyl ether, and step (2) is the same as that of Example 4. The specific process of step (1) is as follows:

[0080] (1) The modified polyvinyl alcohol and tea polyphenols were stirred in a high-speed mixer at 450 r / min and 220° C. for 13 min to form a uniform mixed melt.

[0081] Comparative Example 7: Preparation of protective film

[0082] The steps of this comparative example are the same as those of Example 4, except that the raw materials used in step (1) lack tea polyphenols, and step (2) is the same as that of Example 4. The specific process of step (1) is as follows:

[0083] (1) Modified polyvinyl alcohol and polyethylene glycol monomethyl ether were stirred in a high-speed mixer at 450 r / min and 70° C. for 13 min to form a uniform mixed melt.

[0084] Comparative Example 8: Preparation of protective film

[0085] The steps of this comparative example are the same as those of Example 4, except that the modified polyvinyl alcohol is missing. Step (2) is the same as that of Example 4. The specific process of step (1) is as follows:

[0086] (1) Polyethylene glycol monomethyl ether and tea polyphenols were stirred in a high-speed mixer at 450 r / min and 220° C. for 13 min to form a uniform mixed melt.

[0087] Comparative Example 9: Preparation of protective film

[0088] The steps of this comparative example are the same as those of Example 4, except that the modified polyvinyl alcohol in step (1) is replaced with polyvinyl alcohol, and step (2) is the same as that of Example 4. The specific process of step (1) is as follows:

[0089] (1) Polyvinyl alcohol, polyethylene glycol monomethyl ether and tea polyphenols were mixed in a high-speed mixer at 450 r / min and 70°C for 13 min at 75°C to form a uniform melt.

[0090] 3. Preparation of base fertilizer

[0091] Example 7: Preparation of basal fertilizer

[0092] The base fertilizer used in this embodiment is mixed with the following raw materials:

[0093] 55kg ammonium phosphate, 95kg urea, 25kg dipotassium hydrogen phosphate, 75kg superphosphate, 9kg humic acid, 1.0kg magnesium chloride, and 0.3kg manganese sulfate.

[0094] Interplanting

[0095] Example 8: Interplanting of Artemisia annua and Tea Trees

[0096] (1) Site selection and land preparation: Choose deep, loose, fertile, and well-drained soil for plowing, harrowing, and ploughing. Then build a soil ridge with a width of about 0.7 m and a height of about 40 cm. Divide the soil ridge into two rows at a ratio of 1:1. Dig drainage ditches between the ridges with a width of about 0.5 m and a depth of 0.2 m.

[0097] (2) Artemisia annua planting: The soil in the soil bed was treated by spraying with 800 times carbendazim solution at a spraying amount of 15 kg / mu, and the base fertilizer prepared in Example 7 was applied at an amount of 2000 kg / mu. After an interval of 10 days, Artemisia annua seeds were evenly sown in any row of the soil bed at a sowing density of 0.5 kg per square meter, and a protective film was laid along the soil surface;

[0098] (3) Tea interplanting: After Artemisia annua grows to 10 cm, select strong and uniform-sized tea seedlings of the current year, soak them in yellow mud for 1 hour, and then plant them in the same row of soil where Artemisia annua is not sown;

[0099] (4) Planting and maintenance: Water and fertilizer management should be carried out for Artemisia annua and tea trees. After planting, if dead or diseased seedlings are found, they should be removed in time. Seedlings should be replanted on cloudy or rainy days. Weeding should be carried out according to the growth of weeds after planting. When the tea seedlings grow to 13 cm in height, 50 kg of water should be poured into the base at a temperature of 24 °C. After the watering treatment is completed, the tea seedlings should be topped and branches pruned on a sunny day after 2 days. After the topping and branch pruning are completed, potassium permanganate 800 times diluted should be sprayed at a rate of 17 kg / mu for disinfection, the soil between the rows should be loosened, and base fertilizer should be applied.

[0100] Experiment 1: Interplanting Artemisia annua and tea trees

[0101] 1. Interplanting method and protective film used:

[0102] Experimental group: Experimental group 1 used the method of Example 8 for interplanting, and the protective film was the protective film prepared in Example 4;

[0103] Control group: Control groups 3-9 all adopted the method of Example 8 for interplanting, and the protective films were the protective films prepared in Comparative Examples 3-9 respectively;

[0104] Blank control group: The blank control group did not use a protective film, and the remaining interplanting steps were the same as those of Example 8.

[0105] 4. Experimental steps:

[0106] The intercropping was carried out in the eastern foothills of the Xuefeng Mountains in Hunan Province at an altitude of 800m. The land was divided into 9 plots and planted according to the methods of the experimental group and the control group 3-9. All the above groups started planting in March, and the care methods and fertilizer application amounts of each group were the same.

[0107] During the planting process, the incidence rate of each group of tea trees was tested (incidence rate = number of diseased plants / total number of plants × 100%, where diseased plants are the sum of plants infected with powdery mildew, damping-off and other diseases), Artemisia annua was harvested in August of the same year, and the yield of Artemisia annua in each group and the difference in plant height of tea trees from March to August were counted and recorded as the growth amount. The incidence rate was counted in April of the second year after planting;

[0108] Dry weight of single plant and roots, stems, branches and leaves: Leaves were sun-dried, other parts were sterilized at 105℃ for 30min, and then dried in an oven at 70℃. Each sampling was separated into roots, stems, branches and leaves, dried and weighed separately to obtain the dry weight of roots, stems, branches and leaves. The dry weight of single plant was obtained by adding the dry weights of each part.

[0109] Determination of artemisinin content: ultrasonic extraction and high performance liquid chromatography analysis. Take dry leaves of Artemisia annua at the early stage of budding, crush through a 60-mesh sieve, take 1g and add 40 petroleum ether to ultrasonically extract at room temperature for 45 minutes, cool and fix the volume. Take 20 minutes to evaporate the above extract, dissolve it with anhydrous ethanol and fix the volume for 10 minutes, take 1mL and add 7mL of 0.02% NaoH solution, bathe in 40℃ water for 5 minutes, cool, and fix the volume to 10mL with 0.08mol / L HAc, filter with a microporous filter membrane, and analyze and test with a high performance liquid chromatograph. Repeat three times, the results are shown in Table 3:

[0110] Table 3

[0111]

[0112] 1. The method of interplanting was used in experimental group 1 and control group 3-9 respectively. The statistics of the final products harvested from each group showed that the tea tree in experimental group 1 had the lowest incidence rate, which was 6.48%, and the growth was 34mm. The growth of Artemisia annua was the best, with an average plant height of 114cm, an average single plant weight of 446g, and an artemisinin content of 0.92mg per gram of Artemisia annua. The modified polyvinyl alcohol for preparing the protective film in control group 3 and control group 4 was not treated with glycerol and polyamide epichlorohydrin. The obtained products had strong water absorption capacity, which caused excessive water accumulation in the soil. Both tea trees and Artemisia annua were plants that were afraid of waterlogging, which easily caused root rot. The average plant height and average single plant weight of Artemisia annua were reduced by 23cm and 18cm, 51g and 43g respectively compared with experimental group 1, and the artemisinin content also decreased by 0.24mg / g and 0.2m / g. The incidence rate of tea trees increased by 20.11% and 14.22%, and the growth was 20mm and 24mm.

[0113] 2. The average plant height and average plant weight of Artemisia annua in comparison group 5 were 34 cm and 75 g less than those in experimental group 1, respectively, the artemisinin content decreased by 0.36 mg / g, the incidence of tea trees increased by 40.99%, and the growth amount decreased to 15 mm. The average plant height and average plant weight of Artemisia annua in comparison group 6 were 11 cm and 16 g less than those in experimental group 1, respectively, the artemisinin content decreased by 0.11 mg / g, the incidence of tea trees increased by 5.27%, and the growth amount was 29 mm. Comparison group 5 lacked rice glutenin and monostearate glyceryl, and comparison group 6 lacked polyethylene glycol monomethyl ether in the preparation process of the protective film. It can be seen that both substances played a great role in the preparation process of the protective film in experimental group 1, so the effects of the two comparisons were worse than those of experimental group 1.

[0114] 3. The average plant height and average plant weight of Artemisia annua in comparison group 7 were reduced by 17 cm and 24 g respectively compared with those in experimental group 1, the content of artemisinin decreased by 0.18 mg / g, the incidence of tea trees increased by 10.25%, and the growth amount was 25 mm. Tea polyphenols were not added during the preparation process. The average plant height and average plant weight of Artemisia annua in comparison group 8 were reduced by 32 cm and 67 g respectively compared with those in experimental group 1, the content of artemisinin decreased by 0.32 mg / g, and the incidence of tea trees increased by 33.24%. Comparative Example 8 did not use modified polyvinyl alcohol, and only a gel-like substance was obtained, which could not play a role in protecting the soil. The average plant height and average plant weight of Artemisia annua in comparison example 7 were reduced by 26 cm and 62 g respectively compared with those in experimental group 1, the content of artemisinin decreased by 0.29 mg / g, the incidence of tea trees increased by 28.8%, and the growth amount was 18 mm. The preparation material used in the comparative group 9 is untreated polyvinyl alcohol, which is very easy to dissociate and has a short covering time. The dissociated fragments will be dispersed and remain in the soil, which is not only difficult to collect but also causes soil pollution. Therefore, the modified polyvinyl alcohol of the experimental group 1 is required for preparation.

[0115] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the purpose and scope of the technical solutions of the present invention, which should be included in the scope of the claims of the present invention. The techniques, shapes, and structural parts not described in detail in the present invention are all known technologies.

Claims

1. A method for interplanting Artemisia annua and tea trees, characterized in that: The following steps are involved: (1) Site selection and land preparation: The soil is plowed and leveled, and then piled into soil ridges. The soil ridges are divided into two rows, and drainage ditches are dug between the ridges; (2) Artemisia annua planting: Spray the soil in the soil bed with 800 times carbendazim solution and apply base fertilizer, and evenly sow Artemisia annua seeds at a sowing density of 0.3-0.8 kg per square meter in any row of the soil bed, and lay a protective film; (3) Tea interplanting: After Artemisia annua grows to 8-10 cm, select sturdy and uniform-sized tea seedlings, soak them in yellow mud, and then plant them in another row of the same soil bed where Artemisia annua is not sown; (4) Planting and maintenance: water and fertilizer management for Artemisia annua and tea trees; The protective film comprises the following raw materials: 3-8 parts by mass of glyceryl monostearate, 7-10 parts by mass of rice gluten, 1-2 parts by mass of polyethylene glycol monomethyl ether, 1.4-2 parts by mass of tea polyphenols, 4-5 parts by mass of corn starch, 60-80 parts by mass of modified polyvinyl alcohol, 0.5-1 parts by mass of sodium alginate, 1-1.8 parts by mass of cyclodextrin, and 0.1-0.5 parts by mass of xanthan gum.

2. The interplanting method of Artemisia annua and tea tree according to claim 1, characterized in that: The width of the soil ridge is 0.6-0.8m, and the height is 40cm; the width of the drainage ditch is 0.4-0.6m, and the soil ridge is divided into two rows in a width ratio of 1:

1.

3. The interplanting method of Artemisia annua and tea tree according to claim 1, characterized in that: The base fertilizer is prepared by mixing the following raw materials: 50-60 parts by mass of ammonium phosphate, 80-110 parts by mass of urea, 20-30 parts by mass of dipotassium hydrogen phosphate, 70-80 parts by mass of superphosphate, 7-11 parts by mass of humic acid, 0.9-1.1 parts by mass of magnesium chloride, and 0.1-0.5 parts by mass of manganese sulfate.

4. The interplanting method of Artemisia annua and tea tree according to claim 3, characterized in that: The preparation method of the protective film is as follows: (1) stirring the modified polyvinyl alcohol, polyethylene glycol monomethyl ether and tea polyphenols in a high-speed mixer at a speed of 400-500 r / min for 10-15 min to form a uniform mixed melt; (2) Glyceryl monostearate and rice gluten are added to sodium alginate and an appropriate amount of water, and then cyclodextrin, xanthan gum and corn starch are added after stirring for 20-40 minutes. The mixture is added to the mixed melt, and the mixture is stirred at 170-190° C. at a speed of 100-200 r / min for 20-40 minutes, and then a film is blown to obtain a protective film.

5. The interplanting method of Artemisia annua and tea tree according to claim 4, characterized in that: The modified polyvinyl alcohol comprises the following raw materials: 10-12 parts by weight of polyvinyl alcohol, 20-30 parts by weight of 3wt% sodium hydroxide solution, 5-8 parts by weight of chloroacetyl chloride, 0.3-0.5 parts by weight of N,N-diisopropylethylamine, 0.5-0.7 parts by weight of glycerol, and 3-5 parts by weight of polyamide epichlorohydrin.

6. The interplanting method of Artemisia annua and tea tree according to claim 5, characterized in that: The preparation steps of the modified polyvinyl alcohol are as follows: Dissolve chloroacetyl chloride and N,N-diisopropylethylamine to obtain a mixed solution for standby use; Add 3wt% sodium hydroxide solution to polyvinyl alcohol, heat to 70-85°C after mixing, stir and react at constant temperature for 1-2h, add the mixed solution, keep the temperature for reaction for 40-50min, add propylene glycol and continue to mix and stir for 10-20min, then add polyamide epichlorohydrin, continue to stir for 30-60min, cool and dry to constant weight, grind and crush to obtain modified polyvinyl alcohol.

7. The interplanting method of Artemisia annua and tea tree according to claim 6, characterized in that: The thickness of the protective film is 0.013-0.015 mm.

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