A centrifugal chip and microfluidic system

Abstract

The present invention discloses a centrifugal chip, including an upper chip, a lower chip, a microporous filter membrane, a bottom plate and an adhesive layer, wherein the upper chip, the lower chip and the bottom plate are connected in sequence, and an adhesive layer is arranged between the upper chip and the lower chip; an injection hole is arranged on the upper chip, a liquid reservoir and a first channel are arranged at the bottom of the upper chip, and the injection hole and the first channel are both connected with the liquid reservoir; a filter tank, a waste liquid tank and a second channel are arranged at the top of the lower chip, a microporous filter membrane is arranged on the filter tank, and the waste liquid tank at the bottom of the microporous filter membrane is connected with the waste liquid tank through the second channel, and a bottom plate is arranged at the bottom of the lower chip; slots corresponding to the shapes and quantities of the liquid reservoir, the first channel, the filter tank, the waste liquid tank and the second channel are arranged on the adhesive layer. The advantage of the present invention is that the centrifugal chip can shorten the detection time, does not need to use specialized equipment, and reduces the use cost.

Description

Technical Field

[0001] The invention relates to the technical field of detection equipment, in particular to a centrifugal chip and a microfluidic system. Background Art

[0002] Microfluidics refers to the controlled manipulation of liquids at the micro-nano scale to achieve most operations in general chemical or biological laboratories. Its advantages lie in the small sample volume required, high throughput, and automated control. It has been widely developed as a cutting-edge technology and applied in clinical practice, food or environmental analysis, virus detection, etc. Centrifugal microfluidics technology can usually achieve high-throughput sample analysis, and can be operated automatically without human intervention, without external gas sources and valves, which improves automation while reducing sample contamination. At the same time, the fluid control method is simple, low cost, insensitive to the pH value and ion concentration of the liquid, and is suitable for transporting various types of biological fluids.

[0003] At present, centrifugal microfluidics technology is widely used in integrated nucleic acid extraction and amplification detection equipment. For example, the Chinese invention patent with publication number CN114545013A discloses a fully automatic sampler, which drives the centrifugal chip to rotate through a rotating component to centrifuge the sample, thereby improving the experimental efficiency of the fully automatic sampler. However, the overall structure of the above equipment is complex and requires professional operation. Not only is the detection time long, but the detection cost is also high. Summary of the invention

[0004] The technical problem to be solved by the present invention is how to shorten the detection time and reduce the detection cost.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0006] A centrifugal chip, comprising an upper chip, a lower chip, a microporous filter membrane, a bottom plate and an adhesive layer, wherein the upper chip, the lower chip and the bottom plate are connected in sequence, and an adhesive layer is provided between the upper chip and the lower chip;

[0007] The upper chip is provided with a sampling hole, and the bottom of the upper chip is provided with a liquid storage tank and a first channel, and the sampling hole and the first channel are both connected to the liquid storage tank;

[0008] The top of the lower chip is provided with a filter tank, a waste liquid tank and a second channel, the filter tank is provided with a microporous filter membrane, the waste liquid tank at the bottom of the microporous filter membrane is connected with the waste liquid tank through the second channel, and the bottom of the lower chip is provided with a bottom plate;

[0009] The adhesive layer is provided with slots corresponding to the shapes and numbers of the liquid storage tank, the first channel, the filter tank, the waste liquid tank and the second channel respectively.

[0010] The centrifugal chip is used to separate and enrich samples. The filtration process can be automatically completed without manual intervention on the centrifugal chip. The power source does not need to directly contact the sample, which improves automation while reducing sample contamination. It only needs to compare the product after the reaction with the colorimetric card to convert the number of bacteria that cannot be directly observed by the naked eye into a color signal. The experimental results can be directly observed by the human eye, which greatly shortens the detection time, eliminates the need for specialized equipment, and reduces the cost of use.

[0011] In addition, the centrifugal chip has a simple structure and high throughput, and can achieve separation and enrichment of different target products simply by replacing microporous filter membranes of different specifications.

[0012] Preferably, a fixing groove is also provided on the upper chip.

[0013] Preferably, a sealing ring is provided between the microporous filter membrane and the filter tank.

[0014] Preferably, the cross-sectional shape of the injection hole is circular, rectangular or triangular.

[0015] Preferably, the cross-section of the liquid storage tank is fan-shaped.

[0016] Preferably, the filtration pore diameter of the microporous filter membrane is 0.45 μm or 0.22 μm.

[0017] Preferably, the present invention also provides a microfluidic system using the centrifugal chip, comprising a centrifugal chip and a centrifuge, wherein the centrifugal chip is fixed on the centrifuge, and the centrifuge is driven to cause the microfluid in the centrifugal chip to flow.

[0018] Preferably, the present invention also provides a detection method of a microfluidic system, comprising the following steps:

[0019] Step 1: Inject the sample from the injection hole into the reservoir;

[0020] Step 2: The centrifuge is driven to rotate the centrifugal chip, and the sample flows from the liquid storage tank into the filter tank through the first channel, and the sample is filtered through the microporous filter membrane so that the pathogens remain on the microporous filter membrane, and the remaining waste liquid flows into the waste liquid tank through the second channel for collection;

[0021] Step 3: Inject the test solution from the injection hole into the liquid storage tank;

[0022] Step 4: driving the centrifuge to rotate the centrifugal chip, the test liquid flows from the liquid storage tank through the first channel to the microporous filter membrane, and the centrifugal chip is heated to cause the test liquid to react with the pathogen;

[0023] Step 5: After the test solution reacts with the pathogen, observe the color of the product on the microporous filter membrane and compare it with the colorimetric card to obtain the test result.

[0024] Preferably, the centrifuge speed in step 2 is 500-5000 rpm / s, and the rotation time is 20-100 seconds.

[0025] Preferably, the centrifuge speed in step 4 is 100-3000 rpm / s, and the rotation time is 10-60 seconds.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] The centrifugal chip is used to separate and enrich samples. The filtration process can be automatically completed without manual intervention on the centrifugal chip. The power source does not need to directly contact the sample, which improves automation while reducing sample contamination. It only needs to compare the product after the reaction with the colorimetric card to convert the number of bacteria that cannot be directly observed by the naked eye into a color signal. The experimental results can be directly observed by the human eye, which greatly shortens the detection time, eliminates the need for specialized equipment, and reduces the cost of use.

[0028] In addition, the centrifugal chip has a simple structure and a high throughput, and the separation and enrichment of different target products can be achieved by simply replacing microporous filter membranes 3 of different specifications. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 An exploded view of a centrifugal chip according to an embodiment of the present invention;

[0030] Figure 2 It is a schematic diagram of the structure of the upper chip according to an embodiment of the present invention. DETAILED DESCRIPTION

[0031] In order to facilitate those skilled in the art to understand the technical solution of the present invention, the technical solution of the present invention is further described in conjunction with the accompanying drawings of the specification.

[0032] In this application, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or a communication; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0033] In this application, unless otherwise expressly specified and limited, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of this application, "plurality" means two or more, unless otherwise clearly and specifically limited.

[0034] See also Figure 1 to Figure 2 This embodiment discloses a centrifugal chip, including an upper chip 1, a lower chip 2, a microporous filter membrane 3, a bottom plate 4 and an adhesive layer 5, wherein the upper chip 1, the lower chip 2 and the bottom plate 4 are connected in sequence, and an adhesive layer 5 is arranged between the upper chip 1 and the lower chip 2.

[0035] The upper chip 1 is provided with an injection hole 11, and a liquid storage tank 12 and a first channel 13 are provided at the bottom of the upper chip 1. The injection hole 11 and the first channel 13 are both connected to the liquid storage tank 12. Specifically, the cross-sectional shape of the injection hole 11 is circular, rectangular or triangular, and is circular in the present embodiment, and the cross-sectional shape of the liquid storage tank 12 is fan-shaped.

[0036] A filter tank 21, a waste liquid tank 22 and a second channel 23 are arranged on the top of the lower chip 2, a microporous filter membrane 3 is arranged on the filter tank 21, a sealing ring 6 is arranged between the microporous filter membrane 3 and the filter tank 21, the waste liquid tank 22 at the bottom of the microporous filter membrane 3 is connected to the waste liquid tank 22 through the second channel 23, and a bottom plate 4 is arranged at the bottom of the lower chip 2.

[0037] The upper chip 1 and the lower chip 2 are provided with fixing grooves 14 .

[0038] The filtration pore diameter of the microporous filter membrane 3 is 0.45 μm or 0.22 μm, which is specifically determined according to the diameter of the pathogen in the test sample, ensuring that the filtration pore diameter of the microporous filter membrane 3 is larger than the diameter of the pathogen.

[0039] Furthermore, the upper chip 1, the lower chip 2 and the bottom plate 4 are made of PMMA, PC or PP.

[0040] The adhesive layer 5 is provided with slots corresponding to the shapes and numbers of the liquid storage tank 12, the first channel 13, the filter tank 21, the waste liquid tank 22 and the second channel 23, so that the sample and the detection liquid can enter the filter tank 21 from the liquid storage tank 12 through the first channel 13 for filtration, wherein the waste liquid is filtered by the microporous filter membrane 3 and then flows into the waste liquid tank 22 through the second channel 23 for collection.

[0041] This embodiment also discloses a microfluidic system, including a centrifugal chip and a centrifuge. The centrifugal chip is fixed on the centrifuge, and the centrifuge is driven to cause the microfluid in the centrifugal chip to flow.

[0042] This embodiment also discloses a detection method of a microfluidic system, comprising the following steps:

[0043] Step 1: inject the sample from the injection hole 11 into the liquid storage tank 12;

[0044] Step 2: Drive the centrifuge to drive the centrifuge chip to rotate, the speed of the centrifuge is 500-5000rpm / s, the rotation time is 20-100 seconds, the sample flows from the liquid storage tank 12 into the filter tank 21 through the first channel 13, and the sample is filtered through the microporous filter membrane 3 so that the pathogens remain on the microporous filter membrane 3, and the remaining waste liquid flows into the waste liquid tank 22 through the second channel 23 for collection;

[0045] Step 3: inject the test liquid from the injection hole 11 into the liquid storage tank 12;

[0046] Step 4: driving the centrifuge to rotate the centrifuge chip, the rotation speed of the centrifuge is 100-3000 rpm / s, the rotation time is 10-60 seconds, the detection liquid flows from the liquid storage tank 12 to the microporous filter membrane 3 through the first channel 13, and the centrifugal chip is heated to cause the detection liquid to react with the pathogen. Specifically, the filter tank 21 of the centrifugal chip is heated to maintain the temperature at 37° C. The heating reaction is stopped after 60 minutes;

[0047] Step 5: After the test solution reacts with the pathogen, the color of the product on the microporous filter membrane 3 is observed and compared with the colorimetric card to obtain the test result.

[0048] Step 6: Take a picture of the test result image and process it in the Image software to get the gray value of the image. Substitute the gray value into the pre-calculated standard curve to get the bacterial concentration corresponding to the group of samples, and compare it with the sample concentration pre-recorded in step 2 to verify the feasibility and accuracy of this scheme.

[0049] The centrifugal chip is used to separate and enrich samples. The filtration process can be automatically completed without manual intervention on the centrifugal chip. The power source does not need to directly contact the sample, which improves automation while reducing sample contamination. It only needs to compare the product after the reaction with the colorimetric card to convert the number of bacteria that cannot be directly observed by the naked eye into a color signal. The experimental results can be directly observed by the human eye, which greatly shortens the detection time, eliminates the need for specialized equipment, and reduces the cost of use.

[0050] In addition, the centrifugal chip has a simple structure and a high throughput, and the separation and enrichment of different target products can be achieved by simply replacing microporous filter membranes 3 of different specifications.

[0051] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-limiting from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention, and any reference numerals in the claims should not be regarded as limiting the claims involved.

[0052] The above-described embodiments merely represent implementation methods of the invention. The protection scope of the present invention is not limited to the above-described embodiments. For those skilled in the art, several modifications and improvements may be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A centrifugal chip, characterized in that: It includes an upper chip, a lower chip, a microporous filter membrane, a bottom plate and an adhesive layer, wherein the upper chip, the lower chip and the bottom plate are connected in sequence, and an adhesive layer is provided between the upper chip and the lower chip; The upper chip is provided with a sampling hole, and the bottom of the upper chip is provided with a liquid storage tank and a first channel, and the sampling hole and the first channel are both connected to the liquid storage tank; The top of the lower chip is provided with a filter tank, a waste liquid tank and a second channel, the filter tank is provided with a microporous filter membrane, the filter tank at the bottom of the microporous filter membrane is connected with the waste liquid tank through the second channel, and the bottom of the lower chip is provided with a bottom plate; The adhesive layer is provided with slots corresponding to the shapes and numbers of the liquid storage tank, the first channel, the filter tank, the waste liquid tank and the second channel respectively.

2. A centrifugal chip according to claim 1, characterized in that: The upper chip is also provided with a fixing groove.

3. A centrifugal chip according to claim 1, characterized in that: A sealing ring is arranged between the microporous filter membrane and the filter tank.

4. A centrifugal chip according to claim 1, characterized in that: The cross-sectional shape of the injection hole is circular, rectangular or triangular.

5. A centrifugal chip according to claim 1, characterized in that: The cross-section of the liquid storage tank is fan-shaped.

6. A centrifugal chip according to claim 1, characterized in that: The filtration pore diameter of the microporous filter membrane is 0.45 μm or 0.22 μm.

7. A microfluidic system using the centrifugal chip according to any one of claims 1 to 6, characterized in that: The invention comprises a centrifugal chip and a centrifuge. The centrifugal chip is fixed on the centrifuge. The centrifuge is driven to cause the microfluid in the centrifugal chip to flow.

8. A detection method using a microfluidic system as claimed in claim 7, characterized in that: The steps include: Step 1: Inject the sample from the injection hole into the reservoir; Step 2: The centrifuge is driven to rotate the centrifugal chip, and the sample flows from the liquid storage tank into the filter tank through the first channel, and the sample is filtered through the microporous filter membrane so that the pathogens remain on the microporous filter membrane, and the remaining waste liquid flows into the waste liquid tank through the second channel for collection; Step 3: Inject the test solution from the injection hole into the liquid storage tank; Step 4: driving the centrifuge to rotate the centrifugal chip, the test liquid flows from the liquid storage tank through the first channel to the microporous filter membrane, and the centrifugal chip is heated to cause the test liquid to react with the pathogen; Step 5: After the test solution reacts with the pathogen, observe the color of the product on the microporous filter membrane and compare it with the colorimetric card to obtain the test result.

9. A detection method of a microfluidic system according to claim 8, characterized in that: The centrifuge speed in step 2 is 500-5000 rpm / s, and the rotation time is 20-100 seconds.

10. A detection method of a microfluidic system according to claim 8, characterized in that: The centrifuge speed in step 4 is 100-3000 rpm / s, and the rotation time is 10-60 seconds.

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