A preparation method and application of seaweed feed

Abstract

The invention discloses a preparation method and application of seaweed feed, and relates to the technical field of aquatic feed. The seaweed feed prepared by the invention is prepared by mixing seaweed composite powder prepared by enzymolysis and fermentation of kelp, red algae and brown algae, silkworm pupae with high protein content, blood meal, wheat flour, corn flour, animal bone meal, peanut vine, straw, and a Chinese medicinal feed additive prepared by soaking, decocting, concentrating and drying a mixture of scutellaria baicalensis, almond, liquorice, knotweed, Radix Rehmanniae, Cimicifuga heracleifolia and radix bupleuri. The seaweed feed achieves the effect of efficient utilization of seaweed while ensuring the health, safety, high nutritional value and strong immunity of yellow eel.

Description

Technical Field

[0001] The invention relates to the technical field of aquatic feed, and in particular to a preparation method of seaweed feed and application thereof. Background Art

[0002] Yellow eel is a tropical and warm temperate fish, a bottom-dwelling fish with strong adaptability. It lives at the bottom of the water body, mainly in muddy waters such as rice fields, lakes, ponds, rivers and ditches, and even in swamps, flooded fields or wetlands. It likes to live in caves. The length of the yellow eel cave is about 3 times the length of the body, and the cave is curved and crossed. Each cave usually has more than two caves. The cave exit is often close to the water surface so that it can stick its head out to breathe air. Yellow eel is not only edible but also very nutritious. Eating yellow eel regularly can play a good role in health preservation and health care. With the development of the economy, people's living standards are constantly improving, and the demand for eels is increasing. People are paying more and more attention to the nutritional value and safety factors of eels. In the process of artificial breeding, eels have high requirements for feed, do not eat rotten feed, and require the feed to be fresh. Based on this, the quality of eel feed directly affects its yield. Therefore, today's eel farmers urgently need a feed that can effectively increase eel production and is healthy, safe and of good quality.

[0003] Seaweed is an autotrophic plant that can photosynthesize and convert inorganic substances in the ocean into organic substances. It is a rich and low-cost feed resource for animals. That is, seaweed has great potential for development as a feed raw material and feed additive. At the same time, seaweed contains rich seaweed polysaccharides, proteins, fats, vitamins, minerals and physiologically active substances with special effects. It is a raw material library for food, medicine and feed. Marine plants are rich in nutrients and contain a variety of biologically active substances. They have biological activities such as enhancing the body's immunity, resisting diseases, resisting viruses, and promoting growth. Therefore, the development of marine plant feed and the promotion of animal husbandry are increasingly receiving widespread attention. However, seaweed contains a variety of anti-nutritional ingredients, such as non-starch polysaccharides such as cellulose, saccharide gum, agar, and xylan and their toxins; in addition, aquatic animals that eat seaweed are mostly lower animals with short intestines and poor digestive functions, which greatly affect the digestion and utilization rate of seaweed and cause resource waste. Therefore, how to achieve efficient utilization of seaweed is an urgent problem to be solved. Summary of the invention

[0004] The purpose of the present invention is to provide a method for preparing seaweed feed and its application, so as to solve the problems existing in the above-mentioned prior art, and to achieve the effect of high efficiency utilization of seaweed while ensuring the health, safety, high nutritional value and strong immunity of yellow eel.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] One of the technical solutions of the present invention is to provide a seaweed feed, the raw materials and their mass ratio are: 65-80 parts of seaweed composite powder, 45-50 parts of silkworm pupae, 15-20 parts of blood meal, 30-35 parts of wheat flour, 20-40 parts of corn flour, 15-20 parts of animal bone meal, 3-20 parts of peanut vines, 10-15 parts of rice straw, and 5-10 parts of traditional Chinese medicine feed additives.

[0007] Furthermore, the seaweed composite powder is prepared by using kelp, red algae and brown algae as raw materials through enzymolysis and fermentation.

[0008] Furthermore, the Chinese medicinal feed additive is prepared by taking a mixture of scutellaria baicalensis, apricot kernel, liquorice, polygonum cuspidatum, Radix Rehmanniae, Cimicifuga heracleifolia and radix bupleuri as raw materials through soaking, decocting, concentrating and drying.

[0009] The second technical solution of the present invention provides a method for preparing the above-mentioned seaweed feed, comprising the following steps:

[0010] S1. Preparation of seaweed composite powder:

[0011] S11: preparing the kelp, red algae and brown algae in a mass ratio of 1:0.5-1:1-2, and crushing them to obtain composite algae powder;

[0012] S12. First, cellulase and alginate lyase are dispersed in deionized water to obtain a dispersed solution, and then the dispersed solution is mixed with the composite algae powder, stirred and mixed at a constant temperature of 30°C, and dried to obtain enzymatic algae powder;

[0013] S13. preparing a solid culture medium containing the enzymatic algae powder;

[0014] S14. After inoculating the composite bacterial solution A into the seaweed solid culture medium for aerobic fermentation, the bacterial solution B is then inoculated for anaerobic fermentation to obtain the seaweed composite powder; wherein the bacterial species in the composite bacterial solution A are Bacillus licheniformis and Saccharomyces cerevisiae; and the bacterial species in the bacterial solution B is Lactobacillus acidophilus;

[0015] S2. Preparation of the Chinese herbal feed additive: The scutellaria, almond, licorice, knotweed, Yuanshen, cimicifuga, bupleurum were mixed in the same weight ratio, soaked for 50-65min, decocted for 20-30min to treat the offspring, and the filtered juice was concentrated and dried to obtain a Chinese herbal feed additive;

[0016] S3. Weigh silkworm pupae, blood meal, wheat flour, corn flour, animal bone meal, peanut vines, straw and mix them to obtain a mixture;

[0017] S4. After uniformly mixing the seaweed mixed powder, the Chinese medicinal feed additive and the mixed material, the mixture is placed in a granulator for granulation to obtain seaweed feed.

[0018] Furthermore, in step S12, the mass ratio of the cellulase, alginate lyase and composite algae powder is 1-1.5:1:10.

[0019] Furthermore, the components and mass ratios of the seaweed solid culture medium in step S13 are: 7.5-10 parts of enzymatic algae powder, 2-2.5 parts of bean dregs, 1-1.25 parts of bran, 0.5-1 part of glucose, 0.5-1 part of ammonium sulfate, and 0.5-1 part of potassium dihydrogen phosphate.

[0020] Furthermore, the mass ratio of Bacillus licheniformis to Saccharomyces cerevisiae in the composite bacterial solution A is 1:1.

[0021] Further, the specific steps of inoculation in step S14 are: first, the composite bacterial solution A is inoculated into the seaweed solid culture medium at an inoculation amount of 2.1 v / w%-2.7 v / w%, and aerobically fermented at 30-32° C. for 20-25 hours, and then the bacterial solution B is inoculated at an inoculation amount of 2.3 v / w%-3.3 v / w%, and anaerobically fermented at 35-37° C. for 38-45 hours, wherein the bacterial concentrations of the composite bacterial solution A and the bacterial solution B are both 1.5×10 8 Pieces / mL.

[0022] The third technical solution of the present invention provides the use of the above-mentioned seaweed feed as eel feed.

[0023] Compared with the prior art, the present invention first uses cellulase and alginate lyase to enzymatically hydrolyze a mixed powder of kelp, red algae and brown algae prepared in proportion, and then places the obtained enzymatically hydrolyzed algae powder in a culture medium for fermentation, and combines it with high-protein silkworm pupae and traditional Chinese medicine feed additives to prepare seaweed feed, thereby ensuring that the eel is healthy, safe, has high nutritional value and strong immune ability, while achieving the effect of efficient utilization of seaweed.

[0024] The present invention discloses the following technical effects:

[0025] (1) The present invention uses kelp, red algae and brown algae with good antibacterial ability as raw materials, uses cellulase and alginate lyase to enzymatically hydrolyze the mixed powder of kelp, red algae and brown algae prepared in proportion, and then places the obtained enzymatic hydrolyzed algae powder in a culture medium for fermentation. This method further degrades the crude cellulose, alginic acid and other substances that are difficult to absorb in the seaweed powder, while enhancing the activity of the intestinal flora of the eel, thereby achieving the effect of improving the yield and quality of the eel.

[0026] (2) At the same time, in the fermentation treatment step, the present invention adopts a technical scheme of first using Bacillus licheniformis and Saccharomyces cerevisiae for aerobic fermentation and then using Lactobacillus acidophilus for anaerobic fermentation, which can effectively prevent eel enteritis, decompose toxic and harmful substances in water, promote the degradation of nutrients in feed, adjust intestinal flora imbalance, reduce intestinal bacterial infection, and produce resistance substances to inhibit the growth and reproduction of pathogenic bacteria. At the same time, the above-mentioned composite bacterial liquid can also be used as a probiotic to regulate the intestinal function of animals and enhance the body's immune ability of animals.

[0027] (3) Protein is an important element that affects the quality of yellow eel. In the breeding of yellow eel, too much or too little protein will affect the growth of yellow eel. The present invention adopts silkworm pupae that are easier to digest and absorb to provide yellow eel with sufficient protein, and limits the specific amount of protein added. At the same time, the technical scheme of combining it with seaweed composite powder and traditional Chinese medicine feed additives is adopted to effectively enhance the immune function of yellow eel and improve its disease resistance. It ensures that the yellow eel is healthy, safe, high in nutritional value and strong in immunity, while achieving the effect of effective utilization of seaweed and saving resources. DETAILED DESCRIPTION

[0028] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0029] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0030] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0031] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present application description and examples are exemplary only.

[0032] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0033] Unless otherwise specified, the "parts" described in the present invention are all based on mass parts.

[0034] Example 1

[0035] A seaweed feed, the raw materials of which are the following components in parts by weight:

[0036] 70 parts of seaweed composite powder, 45 parts of silkworm pupae, 15 parts of blood meal, 32 parts of wheat flour, 28 parts of corn flour, 15 parts of animal bone meal, 6 parts of peanut vines, 12 parts of rice straw, and 8 parts of traditional Chinese medicine feed additives.

[0037] Among them, the raw materials of seaweed composite powder are kelp, red algae and brown algae; the raw materials of Chinese medicinal feed additives are a mixture of scutellaria baicalensis, almond, licorice, knotweed, Yuanshen, Cimicifuga heracleifolia and bupleurum.

[0038] The preparation method of seaweed feed in this embodiment is as follows:

[0039] S1. Preparation of seaweed composite powder

[0040] S11. After washing and removing mud from kelp, red algae and brown algae, drying and dehumidifying, crushing and milling, the mixture is mixed in a ratio of 1:1:1 to obtain composite algae powder;

[0041] S12. First, cellulase and alginate lyase are dispersed in deionized water to obtain a dispersed solution, and then the dispersed solution and the composite algae powder are placed in a stirred reactor, stirred at a constant temperature of 30° C. for 3 minutes, and dried to obtain enzymatic algae powder; wherein the mass ratio of the cellulase, alginate lyase and composite algae powder is 1:1:10;

[0042] S13. The enzymatic algae powder is mixed with dregs, bran, glucose, ammonium sulfate and potassium dihydrogen phosphate to prepare a seaweed solid medium, wherein the specific components and mass fractions of the seaweed solid medium are: 8 parts of enzymatic algae powder, 2 parts of dregs, 1 part of bran, 0.5 parts of glucose, 0.8 parts of ammonium sulfate and 0.5 parts of potassium dihydrogen phosphate;

[0043] S14. Inoculate the composite bacterial solution A (containing the bacterial species of Bacillus licheniformis and Saccharomyces cerevisiae in a mass ratio of 1:1) into the above-mentioned seaweed solid culture medium at an inoculation rate of 2.5 v / w%, and ferment aerobically at 30°C for 22 hours. Then inoculate the bacterial solution B (containing the bacterial species of Lactobacillus acidophilus) at an inoculation rate of 2.3 v / w%, and ferment anaerobically at 35°C for 38 hours to obtain seaweed composite powder; wherein the concentrations of the composite bacterial solution A and the bacterial solution B are both 1.5×10 8 Pieces / mL.

[0044] S2. Preparation of Chinese herbal feed additives: Scutellaria, almonds, licorice, knotweed, Yuanshen, Cimicifuga, and Bupleurum were mixed in the same weight ratio, and the mixture was added with 1000 ml of cold water, soaked for 60 min, simmered for 30 min, and the juice was filtered out; then 800 ml of cold water was added and simmered for another 25 min, and the juice filtered out twice was mixed, concentrated to remove water, and dried to obtain a Chinese herbal feed additive;

[0045] S3. Weigh silkworm pupae, blood meal, wheat flour, corn flour, animal bone meal, peanut vines, straw and stir and mix to obtain a mixture;

[0046] S4. After mixing the prepared seaweed composite powder, Chinese medicinal feed additive and mixed material, place them in a granulator for granulation to obtain seaweed feed.

[0047] Example 2

[0048] A seaweed feed, the raw materials of which are the following components in parts by weight:

[0049] 65 parts of seaweed composite powder, 48 parts of silkworm pupae, 18 parts of blood meal, 30 parts of wheat flour, 32 parts of corn flour, 18 parts of animal bone meal, 4 parts of peanut vines, 10 parts of rice straw, and 6 parts of traditional Chinese medicine feed additives.

[0050] Among them, the raw materials of seaweed composite powder are kelp, red algae and brown algae; the raw materials of Chinese medicinal feed additives are a mixture of scutellaria baicalensis, almond, licorice, knotweed, Yuanshen, Cimicifuga heracleifolia and bupleurum.

[0051] The preparation method of seaweed feed in this embodiment is as follows:

[0052] S1. Preparation of seaweed composite powder

[0053] S11. After washing and removing mud, drying and dehumidifying the kelp, red algae and brown algae, crushing and milling the kelp, the composite algae powder is prepared and mixed in a ratio of 1:0.5:1;

[0054] S12. First, cellulase and alginate lyase are dispersed in deionized water to obtain a dispersed solution, and then the dispersed solution and the composite algae powder are placed in a stirred reactor, stirred at a constant temperature of 30° C. for 4 minutes, and dried to obtain enzymatic algae powder; wherein the mass ratio of the cellulase, alginate lyase and composite algae powder is 1.2:1:10;

[0055] S13. The enzymatic algae powder is mixed with dregs, bran, glucose, ammonium sulfate and potassium dihydrogen phosphate to prepare a seaweed solid medium, wherein the specific components and mass fractions of the seaweed solid medium are: 7.5 parts of enzymatic algae powder, 2.5 parts of dregs, 1 part of bran, 0.6 parts of glucose, 0.6 parts of ammonium sulfate and 0.8 parts of potassium dihydrogen phosphate;

[0056] S14. The composite bacterial solution A (containing the bacterial species of Bacillus licheniformis and Saccharomyces cerevisiae in a mass ratio of 1:1) was aerobically fermented at 31°C for 20 h, and then the bacterial solution B (containing the bacterial species of Lactobacillus acidophilus) was inoculated at an inoculum amount of 3.0 v / w%, and anaerobically fermented at 37°C for 40 h to obtain seaweed composite powder; wherein the concentrations of the composite bacterial solution A and the bacterial solution B were both 1.5×10 8 Pieces / mL.

[0057] S2. Preparation of Chinese herbal feed additives: Scutellaria, almonds, licorice, Polygonum cuspidatum, Yuanshen, Cimicifuga, and Bupleurum were mixed in the same weight ratio, and the mixture was added with 800 ml of cold water, soaked for 65 min, simmered for 25 min, and the juice was filtered out; then 600 ml of cold water was added and simmered for another 30 min, and the juice filtered out twice was mixed, concentrated to remove water, dried, and then crushed to obtain a Chinese herbal feed additive;

[0058] S3. Weigh silkworm pupae, blood meal, wheat flour, corn flour, animal bone meal, peanut vines, straw and stir and mix to obtain a mixture;

[0059] S4. After mixing the prepared seaweed composite powder, Chinese medicinal feed additive and mixed material, place them in a granulator for granulation to obtain seaweed feed.

[0060] Example 3

[0061] A seaweed feed, the raw materials of which are the following components in parts by weight:

[0062] 80 parts of seaweed composite powder, 46 parts of silkworm pupae, 17 parts of blood meal, 33 parts of wheat flour, 29 parts of corn flour, 16 parts of animal bone meal, 13 parts of peanut vines, 15 parts of rice straw, and 9 parts of traditional Chinese medicine feed additives.

[0063] Among them, the raw materials of seaweed composite powder are kelp, red algae and brown algae; the Chinese medicinal feed additives are a mixture of scutellaria baicalensis, almond, licorice, knotweed, Yuanshen, Cimicifuga heracleifolia and bupleurum.

[0064] The preparation method of seaweed feed in this embodiment is as follows:

[0065] S1. Preparation of seaweed composite powder

[0066] S11. After washing and removing mud from kelp, red algae and brown algae, drying and dehumidifying, crushing and milling, the composite algae powder is prepared and mixed in a ratio of 1:1:2;

[0067] S12. First, cellulase and alginate lyase are dispersed in deionized water to obtain a dispersed solution, and then the dispersed solution and the composite algae powder are placed in a stirred reactor, stirred at a constant temperature of 30° C. for 5 minutes, and dried to obtain enzymatic algae powder; wherein the mass ratio of the cellulase, alginate lyase and composite algae powder is 1.5:1:10;

[0068] S13. The enzymatic algae powder is mixed with dregs, bran, glucose, ammonium sulfate and potassium dihydrogen phosphate to prepare a seaweed solid medium, wherein the specific components and mass fractions of the seaweed solid medium are: 9 parts of enzymatic algae powder, 2 parts of dregs, 1.25 parts of bran, 0.8 parts of glucose, 0.5 parts of ammonium sulfate and 0.7 parts of potassium dihydrogen phosphate;

[0069] S14. Inoculate the composite bacterial solution A (containing the bacterial species of Bacillus licheniformis and Saccharomyces cerevisiae in a mass ratio of 1:1) into the above-mentioned seaweed solid culture medium at an inoculation amount of 2.5 v / w%, and ferment aerobically at 32°C for 25 h. Then inoculate the bacterial solution B (containing the bacterial species of Lactobacillus acidophilus) at an inoculation amount of 2.8 v / w%, and ferment anaerobically at 36°C for 42 h to obtain seaweed composite powder; wherein the concentrations of the composite bacterial solution A and the bacterial solution B are both 1.5×10 8 Pieces / mL.

[0070] S2. Preparation of Chinese herbal feed additives: Scutellaria, almonds, licorice, Polygonum cuspidatum, Yuanshen, Cimicifuga, and Bupleurum were mixed in the same weight ratio, and the mixture was added with 900 ml of cold water, soaked for 50 min, simmered for 20 min, and the juice was filtered out; then 700 ml of cold water was added and simmered for another 20 min, and the juice filtered out twice was mixed, concentrated to remove water, dried, and then crushed to obtain a Chinese herbal feed additive;

[0071] S3. Weigh silkworm pupae, blood meal, wheat flour, corn flour, animal bone meal, peanut vines, straw and stir and mix to obtain a mixture;

[0072] S4. After mixing the prepared seaweed composite powder, Chinese medicinal feed additive and mixed material, place them in a granulator for granulation to obtain seaweed feed.

[0073] Comparative Example 1

[0074] The only difference from Example 1 is that the raw material added in step S4 is composite algae powder that has not been subjected to enzymatic hydrolysis and fermentation treatment. The specific steps are as follows:

[0075] S1. After washing and removing mud, drying and dehumidifying the kelp, red algae and brown algae, crushing and powdering the kelp, the composite algae powder is prepared and mixed in a ratio of 1:1:1;

[0076] S2. Preparation of Chinese herbal feed additives: Scutellaria, almonds, licorice, knotweed, Yuanshen, Cimicifuga, and Bupleurum were mixed in the same weight ratio, and the mixture was added with 1000 ml of cold water, soaked for 60 min, simmered for 30 min, and the juice was filtered out; then 800 ml of cold water was added and simmered for another 25 min, and the juice filtered out twice was mixed, concentrated to remove water, and dried to obtain a Chinese herbal feed additive;

[0077] S3. Weigh silkworm pupae, blood meal, wheat flour, corn flour, animal bone meal, peanut vines, straw and stir and mix to obtain a mixture;

[0078] S4. After mixing the prepared composite algae powder, Chinese medicinal feed additives and mixed materials, place them in a granulator for granulation to obtain seaweed feed.

[0079] Comparative Example 2

[0080] The only difference from Example 1 is that no Chinese medicinal feed additive is added.

[0081] Comparative Example 3

[0082] The only difference from Example 1 is that no silkworm pupae were added.

[0083] Effect verification

[0084] Analysis of growth indicators

[0085] The seaweed feed prepared in the above examples and the seaweed feed prepared in the comparative example were used to carry out a breeding experiment on yellow eel. In the breeding experiment, a pond was divided into 6 sections of 500 m in size by a plastic fence. 2 of small ponds were used as six treatment groups, and the feeds prepared in Examples 1-3 and Comparative Examples 1-3 were fed respectively. At the same time, during the breeding process, normal feeding began after the water temperature rose to 15°C, wherein the daily feeding amount of each group of feed was 2% to 5% of the total weight of the eel, and the eel was fed once every evening, and the feeding amount was adjusted in time according to the weather conditions. In the early stage of breeding, the water level was controlled at about 1m, and water was added and changed once a week to half a month; in the high temperature season, the water level was increased to 1.3 to 1.5m, and water was added (changed) once or twice a week, each time about 20 to 40cm or about 1 / 4 of the total water volume in the pond. Regularly check the water quality of the eel pond, control the dissolved oxygen in the water to be above 3mg / L, the pond pH = 7.0 to 8.5, and the ammonia nitrogen (NH 4+-N)<0.2mg / L, nitrite (NO 2 --N)<0.1mg / L, hydrogen sulfide (S 2- )<0.001mg / L. The eels used in the experiment were purchased from a certain eel breeding base. The seedlings were healthy and the size was (10.01±1.02)g. The stocking density was 20,000 / hm 2 , the male-female ratio is 1:1.

[0086] After 6 months of breeding according to the above method, breeding was terminated, and 20 eels were randomly taken from each test pond, and the body weight was weighed and recorded. Then, live dissection was performed, muscle tissue was taken, and the meat rate (%) index was calculated. Meat rate (%) = muscle weight / eel body weight × 100, and the above experimental data were recorded in Table 1.

[0087] Table 1

[0088] <![CDATA[Yield (kg / hm 2 )]]> Body mass (kg) Meat yield / % Example 1 9653.2 0.66 30.03 Example 2 9631.2 0.63 28.93 Example 3 9613.3 0.59 29.56 Comparative Example 1 7965.6 0.46 20.93 Comparative Example 2 8123.5 0.39 19.82 Comparative Example 3 8031.2 0.42 21.05

[0089] It can be seen from Table 1 that the yield, body weight and meat yield of the eels prepared by the feeds of Examples 1-3 of the present invention are higher in comparison.

[0090] Immune index analysis

[0091] The immune response of eels mainly relies on nonspecific immunity. Nonspecific immune enzymes play an important role in the body's defense system. The antioxidant capacity of immune enzymes such as superoxide dismutase, catalase and glutathione peroxidase indirectly reflects the immune status of the animal body.

[0092] Analysis of antioxidant enzyme activity: Serum from the intestinal lymphoid tissue of the eel was extracted and placed in an Eppendorf tube at 4°C overnight. After centrifugation in a high-speed refrigerated centrifuge, the serum was aspirated and stored at -85°C for testing. The total antioxidant capacity T-AOC, superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) activities of the serum in the intestine were measured using commercially available kits and recorded in Table 2.

[0093] Table 2

[0094] T-AOC (U / mg) SOD(U / mg) MDA (mmol / mg) CAT (U / mg) Example 1 0.32 131.06 0.18 7.86 Example 2 0.29 129.65 0.16 7.25 Example 3 0.31 130.92 0.15 7.45 Comparative Example 1 0.14 91.36 0.12 6.95 Comparative Example 2 0.12 87.69 0.10 6.52 Comparative Example 3 0.13 94.24 0.11 6.85

[0095] It can be seen from Table 2 that the technical solution of the present invention can regulate the intestinal function of the eel, effectively enhance the immune function of the eel, improve the disease resistance of the eel, and thus achieve the effect of improving the yield and quality of the eel.

[0096] In summary, the present invention first uses cellulase and alginate lyase to enzymatically hydrolyze the composite algae powder prepared in proportion by kelp, red algae and brown algae, and then places the obtained enzymatically hydrolyzed algae powder in a culture medium for fermentation, and combines it with high-protein silkworm pupae and traditional Chinese medicine feed additives to prepare seaweed feed, thereby achieving the effect of efficient utilization of seaweed while ensuring the health, safety, high nutritional value and strong immunity of the eel.

[0097] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A seaweed feed, which is used as a rice field eel feed, characterized in that: The raw materials and their mass ratio are as follows: 70 parts of seaweed composite powder, 45 parts of silkworm pupae, 15 parts of blood meal, 32 parts of wheat flour, 28 parts of corn flour, 15 parts of animal bone meal, 6 parts of peanut vines, 12 parts of straw, and 8 parts of traditional Chinese medicine feed additives in terms of mass fraction; the seaweed composite powder is prepared by enzymolysis and fermentation of kelp, red algae and brown algae; The Chinese medicinal feed additive is prepared by taking a mixture of scutellaria baicalensis, apricot kernel, liquorice, polygonum cuspidatum, radix polygoni ginseng, cimicifuga heracleifolia and radix bupleuri as raw materials through soaking, decocting, concentrating and drying.

2. The method for preparing seaweed feed according to claim 1, characterized in that: The following steps are involved: S1. Preparation of seaweed composite powder: S11. The kelp, red algae and brown algae are prepared in a mass ratio of 1:0.5-1:1-2, crushed to obtain a composite algae powder; S12. First, cellulase and alginate lyase are dispersed in deionized water to obtain a dispersed solution, and then the dispersed solution is mixed with the composite algae powder, stirred and mixed at a constant temperature of 30°C, and dried to obtain enzymatic algae powder; S13. preparing a solid culture medium containing the enzymatic algae powder; S14. After inoculating the composite bacterial solution A into the seaweed solid culture medium for aerobic fermentation, the bacterial solution B is then inoculated for anaerobic fermentation to obtain the seaweed composite powder; wherein the bacterial species in the composite bacterial solution A are Bacillus licheniformis and Saccharomyces cerevisiae; and the bacterial species in the bacterial solution B is Lactobacillus acidophilus; The specific steps of inoculation in step S14 are: first, the composite bacterial solution A is inoculated into the seaweed solid culture medium at an inoculation amount of 2.1 v / w%-2.7 v / w%, and aerobically fermented at 30-32°C for 20-25 hours, and then the bacterial solution B is inoculated at an inoculation amount of 2.3 v / w%-3.3 v / w%, and anaerobically fermented at 35-37°C for 38-45 hours; wherein the bacterial concentrations of the composite bacterial solution A and the bacterial solution B are both 1.5×10 8 Pieces / ml; S2. Preparation of the Chinese herbal feed additive: The scutellaria, almond, licorice, knotweed, Yuanshen, cimicifuga, bupleurum were mixed in the same weight ratio, soaked for 50-65min, decocted for 20-30min, and the filtered juice was concentrated and dried to obtain a Chinese herbal feed additive; S3. Weigh the silkworm pupae, blood meal, wheat flour, corn flour, animal bone meal, peanut vines, straw and mix them to obtain a mixture; S4. After uniformly mixing the seaweed composite powder, the Chinese medicinal feed additive and the mixed material, the mixture is placed in a granulator for granulation to obtain seaweed feed.

3. The method for preparing seaweed feed according to claim 2, characterized in that: The mass ratio of Bacillus licheniformis to Saccharomyces cerevisiae in the composite bacterial solution A is 1:

1.

4. Use of the seaweed feed as claimed in claim 1 as eel feed.