A rapid sampling needle device

Abstract

The present application relates to the technical field of electrospray mass spectrometry analysis devices, and in particular to a rapid sampling spray needle device, which comprises a nanospray needle and a nanospray box disposed on the rear end of the nanospray needle; a negative pressure chamber connected to the rear end of the nanospray needle is disposed in the nanospray box, and the cavity wall of the nanospray box at the negative pressure chamber is made of a deformable and restorable material. The present application has the effect of facilitating the nanospray needle to simply and quickly take samples.

Description

Technical Field

[0001] The present application relates to the technical field of electrospray mass spectrometry analysis devices, and in particular to a rapid sampling spray needle device. Background Art

[0002] Electrospray ionization is a soft ionization technology for producing gas-phase ions. It is also a conventional ion source for mass spectrometry. It is suitable for the ionization of small molecules with medium to strong polarity and macromolecules such as proteins, and is widely used in electrospray mass spectrometry.

[0003] Electrospray mass spectrometry is a mass spectrometry analysis method with an electrospray ionization system. The electrospray ionization system can make the sample solution spray out from the tip of the spray needle to form many tiny charged droplets. Under the action of the electric field, the charged droplets drift to the entrance of the mass spectrometer, and the droplets evaporate quickly, and the charge concentration on the droplet surface increases. When the Coulomb repulsion and the limit value of the droplet surface tension are equal, the droplet will burst into smaller droplets until the droplet becomes very small. Since the curvature radius of the droplet is very small, the surface charge density of the droplet is very large, and a very strong electric field will be formed on the droplet surface. This electric field is sufficient to resolve ions from the droplet, so that the ions can be subsequently manipulated and analyzed in the mass spectrometer. Ion manipulation includes ion fragmentation, ion-molecule reaction, ion-ion reaction, etc., so as to achieve further molecular structure information of the ions. Ion analysis includes mass-to-charge ratio analysis of ions using magnetic analyzers, quadrupole analyzers, time-of-flight analyzers, ion trap analyzers, Fourier transform ion cyclotron resonance analyzers, Fourier transform electric ion trap analyzers, etc.

[0004] Compared with electrospray ionization, nanoelectrospray ionization uses a metal, glass or quartz spray needle with a diameter of less than 100 microns, namely a nanospray needle. The nanospray needle is in the shape of a needle tube, which can form smaller charged droplets in the electrospray ionization system, greatly improving the sensitivity of mass spectrometry analysis while reducing the amount of single sample solution used. When other conditions remain unchanged, the smaller the diameter of the nanospray needle, the higher the sensitivity of the mass spectrometry analysis.

[0005] In the prior art, one end of the offline nanospray needle is a tip, and the end of the nanospray needle away from the tip is a rear end. The caliber of the tip is the caliber of the nanospray needle. For the nanospray needle that can be offline, the conventional sampling method is to inhale the sample solution through an extremely thin and long gun head or a micro-syringe, insert it into the nanospray needle from the rear end of the nanospray needle, inject the sample from the gun head or the micro-syringe into the nanospray needle, and gently shake the nanospray needle so that the sample solution reaches a position close to the tip to complete the nanospray needle sampling. The advantage of this sampling method is that the sampling speed is fast, and the disadvantage is that the extremely thin and long gun head or micro-syringe needs to be finely operated when aligning the sample with the rear end of the nanospray needle. In many cases, due to the length of the extremely thin and long end of the sampler, multiple sample loading is required; when shaking the nanospray needle, certain operating experience is required, and the sample solution is easily thrown out from the tip of the nanospray needle, making the sampling method more cumbersome and complicated; and due to process limitations, it costs a lot to manufacture extremely thin and long gun heads or micro-syringes, making the overall cost of nanoliter electrospray ionization high.

[0006] Another sampling method is siphon sampling, where the tip of the nanospray needle is immersed in the sample solution, and the sample solution is siphoned for a period of time. When the siphon volume of the sample solution in the nanospray needle reaches the required volume, the nanospray needle is taken out to complete the sampling. The advantage of this sampling method is that there is no need to swing the nanospray needle. The disadvantage is that it is limited by the siphon principle. This sampling method has requirements for the caliber of the nanospray needle and cannot be applied to nanospray needles with smaller calibers. Moreover, the smaller the caliber, the longer the waiting time is, which makes it take longer for the nanospray needle to complete the sampling.

[0007] In view of the above-mentioned related technologies, the inventors believe that it is necessary to be able to simply and quickly complete sampling for the nanospray needle. Summary of the invention

[0008] In order to facilitate simple and rapid sampling by a nanospray needle, the present application provides a rapid sampling spray needle device.

[0009] The present application provides a rapid sampling needle device using the following technical solutions:

[0010] A rapid sampling spray needle device, comprising a nanospray needle and a nanospray box arranged on the rear end of the nanospray needle;

[0011] A negative pressure chamber connected to the rear end of the nanospray needle is arranged in the nanospray box, and the cavity wall of the nanospray box at the negative pressure chamber is made of a deformable and restorable material.

[0012] By adopting the above technical solution, the cavity wall of the negative pressure cavity is pressed to cause it to deform, thereby compressing the negative pressure cavity, immersing the tip of the nanospray needle in the sample solution, loosening the cavity wall of the negative pressure cavity, and utilizing the property that the cavity wall of the negative pressure cavity can recover to generate negative pressure in the negative pressure cavity. When the rear end of the nanospray needle is connected to the negative pressure cavity, the sample solution can be simply and quickly absorbed through the tip of the nanospray needle, so that the nanospray needle can simply and quickly take samples.

[0013] Optionally, a vent connected to the negative pressure chamber is provided on the nanospray box or the nanospray needle.

[0014] By adopting the above technical solution, when the cavity wall of the negative pressure cavity is pressed to compress the negative pressure cavity, the diameter of the nanospray needle is small, so that the nanospray needle exhausts slowly, resulting in a slow compression speed of the negative pressure cavity, and then a slow sampling speed. The vent is opened, and the gas in the negative pressure cavity can be discharged faster through the vent, which is convenient for compressing the negative pressure cavity and improving the sampling speed. When the cavity wall of the negative pressure cavity is loosened to generate negative pressure in the negative pressure cavity, the vent needs to be blocked by hand so that the nanospray needle can extract the sample solution. After opening the vent, the negative pressure cavity can stop generating negative pressure at any time, so that the nanospray needle can stop sampling at any time, which is convenient for controlling the sampling volume of the nanospray needle, and preventing the sample solution from being too far away from the tip of the nanospray needle when it is in the nanospray needle, so as to avoid affecting the subsequent process of nanoliter electrospray ionization.

[0015] Optionally, the nanospray box includes a sealed cavity section for opening the negative pressure cavity, and a pressing portion for compressing the negative pressure cavity is provided on the sealed cavity section, the pressing portion is made of a deformable and restorable material, and the vent is opened on the pressing portion.

[0016] By adopting the above technical solution, the pressing part is made of a deformable and restorable material, so that the vent is opened and a force is applied to the pressing part, so that the pressing part is deformed to compress the negative pressure chamber. The vent is provided on the pressing part, and the vent can be conveniently blocked. The pressing part restores to generate negative pressure in the negative pressure chamber, or the vent can be opened at any time, the negative pressure chamber stops generating negative pressure, and the nanospray needle stops sampling at any time.

[0017] Optionally, the nanospray box also includes a mounting section for fixing the nanospray needle, the mounting section is at one end of the sealed cavity section close to the nanospray needle, a needle insertion channel connected to the negative pressure cavity is opened on the mounting portion, and the rear end of the nanospray needle is inserted in the needle insertion channel.

[0018] By adopting the above technical solution, the rear end of the nanospray needle is inserted into the needle insertion channel, so that the rear end of the nanospray needle is fixedly connected to the mounting section. At the same time, the rear end of the nanospray needle is connected to the negative pressure chamber, and the nanospray needle can be detachably connected to the mounting section, keeping the outer diameter of the nanospray needle unchanged, and only changing the caliber of the tip of the nanospray needle. The mounting section can be adapted to install nanospray needles of various calibers.

[0019] Optionally, the nanospray needle seal is disposed in the pin hole, and sealing methods include thermoplastic sealing, interference fit sealing, elastic material sealing, adhesive sealing and sintering sealing.

[0020] By adopting the above technical solution, the nanospray needle is sealed in the needle insertion channel, the vent is blocked, and the negative pressure generated by the negative pressure chamber can fully act on the nanospray needle, making it easier for the nanospray needle to absorb the sample solution.

[0021] Optionally, a mounting sealing ring is sleeved on the nanospray needle, and the outer wall of the mounting sealing ring is fixedly connected to the inner wall of the needle insertion channel.

[0022] By adopting the above technical solution, the installation sealing ring is fixedly connected to the inner wall of the pin hole, and the installation sealing ring is sleeved on the nanospray needle. The installation sealing ring plays a sealing role, sealing the gap between the nanospray needle and the pin hole. After the negative pressure chamber generates negative pressure, it is convenient for the nanospray needle to absorb the sample solution. At the same time, the nanospray needle is inserted in the installation sealing ring, and the nanospray needle can be conveniently detachably connected to the installation section, which is convenient for replacing nanospray needles of different calibers.

[0023] Optionally, a needle tip protection structure is provided on one end of the mounting section away from the sealing cavity section, and the needle tip protection structure is arranged around the periphery of the nanospray needle.

[0024] By adopting the above technical solution, the needle tip protection structure is distributed around the nanospray needle, and the needle tip protection structure can protect the nanospray needle and reduce the probability of the nanospray needle being damaged.

[0025] Optionally, it further includes a conductive component, wherein the conductive component includes an electrode for connecting to a power source and a conductive structure electrically connected to the electrode, wherein the conductive structure can generate an electric field covering the sample solution;

[0026] The nanospray box is provided with a docking surface for abutting against a mass spectrometer, and the electrode is arranged on the docking surface.

[0027] By adopting the above technical solution, after the docking surface is abutted against the mass spectrometer, it is convenient for the electrode to be electrically connected to the power supply, and the conductive structure is electrically connected to the electrode. After the power supply is supplied, the conductive structure can generate an electric field, and the electric field covers the sample solution in the nanospray needle, so that the sample solution forms an electrospray and is sprayed from the tip.

[0028] Optionally, the conductive structure is disposed inside the nanospray needle; or the conductive structure is disposed outside the nanospray needle; or the nanospray needle itself constitutes the conductive structure.

[0029] By adopting the above technical solution, the relative positions of the conductive structure and the nanospray needle can be set as needed, so that the conductive structure can be set as needed. When the nanospray needle itself constitutes the conductive structure, the structure of the device can be simpler and easier to manufacture.

[0030] Optionally, when the conductive structure is disposed in the nanospray needle, the conductive structure is a conductive wire made of a conductive material, and the conductive wire is inserted in the nanospray needle;

[0031] When the conductive structure is disposed outside the nanospray needle, the conductive structure is a conductive film made of a conductive material, and the conductive film is coated on the outer wall of the nanospray needle;

[0032] When the nanospray needle itself constitutes the conductive structure, the nanospray needle is a nanospray needle made of a conductive material.

[0033] By adopting the above technical scheme, the conductive wire is used as the conductive structure, and the conductive wire can be inserted in the nanospray needle, which is convenient for replacing the nanospray needle or the conductive wire separately. The conductive film is used as the conductive structure, and the conductive film can be coated on the outer wall of the nanospray needle to prevent the conductive film from affecting the nanospray needle to absorb or spray the sample solution. The nanospray needle itself is used as the conductive structure, so that the nanospray needle and the conductive structure are integrated, and the structure is simpler.

[0034] In summary, the present application includes at least one of the following beneficial technical effects:

[0035] 1. By setting a nanospray needle, a nanospray box and a negative pressure chamber, the cavity wall of the negative pressure chamber is pressed to deform it, and the negative pressure chamber can be compressed. The tip of the nanospray needle is immersed in the sample solution, and the cavity wall of the negative pressure chamber is loosened. The cavity wall of the negative pressure chamber can be restored to generate negative pressure in the negative pressure chamber. When the rear end of the nanospray needle is connected to the negative pressure chamber, the sample solution can be simply and quickly absorbed through the tip of the nanospray needle, so that the nanospray needle can simply and quickly take samples;

[0036] 2. By setting up the vent, when the wall of the negative pressure chamber is pressed to compress the negative pressure chamber, the diameter of the nanospray needle is small, so that the nanospray needle exhausts slowly, resulting in a slow compression speed of the negative pressure chamber, and then a slow sampling speed. The vent is opened, and the gas in the negative pressure chamber can be discharged faster through the vent, which is convenient for compressing the negative pressure chamber and increasing the sampling speed. At the same time, when the wall of the negative pressure chamber is loosened to generate negative pressure in the negative pressure chamber, the vent needs to be blocked by hand so that the nanospray needle can extract the sample solution. After the vent is opened, the negative pressure chamber can stop generating negative pressure at any time, so that the nanospray needle can stop sampling at any time, which is convenient for controlling the sampling volume of the nanospray needle;

[0037] 3. By setting the installation section, the pin hole and the installation sealing ring, the installation sealing ring is fixedly connected to the inner wall of the pin hole, the installation sealing ring is sleeved on the nanospray needle, and the installation sealing ring plays a sealing role, sealing the gap between the nanospray needle and the pin hole. After the negative pressure chamber generates negative pressure, it is convenient for the nanospray needle to absorb the sample solution. At the same time, the nanospray needle is inserted in the installation sealing ring, and the nanospray needle can be conveniently detachably connected to the installation section, which is convenient for replacing nanospray needles of different calibers. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is a schematic diagram of the structure of a rapid sampling needle device in Example 1 of the present application.

[0039] Figure 2 It is a cross-sectional schematic diagram of a rapid sampling needle device in Example 1 of the present application.

[0040] Figure 3 yes Figure 2 Enlarged schematic diagram of part A.

[0041] Figure 4 It is a schematic diagram of the structure of a rapid sampling needle device in Example 2 of the present application.

[0042] Figure 5 It is a cross-sectional schematic diagram of a rapid sampling needle device in Example 2 of the present application.

[0043] Figure 6 yes Figure 5 Schematic diagram of the enlarged portion B.

[0044] Explanation of the reference numerals in the accompanying drawings: 1. Nanospray needle; 2. Nanospray box; 21. Negative pressure chamber; 22. Sealed chamber section; 23. Pressing portion; 24. Mounting section; 25. Pin insertion channel; 26. Needle tip protection structure; 27. Docking surface; 3. Vent; 4. Install sealing ring; 5. Conductive component; 51. Electrode; 52. Conductive structure; 6. Adhesive. DETAILED DESCRIPTION

[0045] The following is combined with Figure 1-6 This application is described in further detail.

[0046] The embodiment of the present application discloses a rapid sampling needle device.

[0047] Embodiment 1:

[0048] Reference Figure 1 and Figure 2 A rapid sampling spray needle device includes a nanospray needle 1 with a hollow interior, a nanospray box 2 arranged on one end of the nanospray needle 1, and a conductive component 5 arranged on the nanospray box 2.

[0049] The nanospray needle 1 is made of metal, glass or quartz, and is in the shape of a needle tube. The end of the nanospray needle 1 away from the nanospray box 2 is the tip, and the end of the nanospray needle 1 away from the tip is the rear end. The caliber of the tip is the caliber of the nanospray needle 1, and the length of the nanospray needle 1 is set as needed.

[0050] The shape of the nanospray box 2 is set as needed. In the present embodiment, the nanospray box 2 is a rectangular parallelepiped. In another embodiment, the nanospray box 2 may also be a long cylindrical shape.

[0051] The nanospray box 2 includes a mounting section 24 detachably connected to the rear end of the nanospray needle 1 and a sealing cavity section 22 fixedly connected to the end of the mounting section 24 away from the nanospray needle 1. The central axis of the nanospray box 2 coincides with that of the nanospray needle 1, and the mounting section 24 and the sealing cavity section 22 are integrally formed.

[0052] Reference Figure 2 and Figure 3 A pin insertion channel 25 for inserting the nanospray needle 1 is opened in the installation section 24 along the length direction of the nanospray box 2, and a negative pressure chamber 21 connected to the pin insertion channel 25 is opened in the sealed chamber section 22. The opening of the pin insertion channel 25 away from the negative pressure chamber 21 is on the end surface of the installation section 24 away from the sealed chamber section 22.

[0053] The rear end of the nanospray needle 1 is sealed in the pin hole 25, and the sealing methods include thermoplastic sealing, interference fit sealing, elastic material sealing, adhesive sealing and sintering sealing. The sealing setting can make the negative pressure generated by the negative pressure chamber 21 act entirely on the nanospray needle 1, so that the nanospray needle 1 can absorb the sample solution. In this embodiment, a mounting seal ring 4 is provided in the pin hole 25, and at least two mounting seal rings 4 are provided, and the two mounting seal rings 4 are fixedly connected to the inner wall of the pin hole 25 at intervals.

[0054] The nanospray needle 1 is inserted into the needle insertion channel 25, and the mounting sealing ring 4 can be sleeved on the outer wall of the nanospray needle 1, so that the nanospray needle 1 can be detachably connected to the mounting section 24. When the outer diameter of the nanospray needle 1 remains unchanged and the caliber of the tip of the nanospray needle 1 is changed, the mounting section 24 can be adapted to install nanospray needles 1 of various calibers.

[0055] Reference Figure 2 and Figure 3 The rear end of the nanospray needle 1 is connected to the negative pressure chamber 21, the length of the nanospray needle 1 is greater than the length of the mounting section 24, and the tip of the nanospray needle 1 is outside the mounting section 24. The two mounting seal rings 4 can not only fix the nanospray needle 1 more firmly on the mounting section 24, but also form a double seal to seal the gap between the nanospray needle 1 and the needle insertion channel 25. After the negative pressure chamber 21 generates negative pressure, the tip of the nanospray needle 1 is immersed in the sample solution, and the sample solution can be easily and quickly absorbed into the nanospray needle 1.

[0056] A pressing portion 23 for compressing the negative pressure cavity 21 is provided on the sealing cavity section 22. The pressing portion 23 is made of a deformable and restorable material. In this embodiment, the pressing portion 23 is made of hard plastic. When a force is applied to the pressing portion 23, the pressing portion 23 is deformed and sunken into the negative pressure cavity 21, so that the negative pressure cavity 21 is compressed.

[0057] Reference Figure 2 and Figure 3A vent 3 connected to the negative pressure chamber 21 is provided on the nanospray box 2 or the nanospray needle 1. In the present embodiment, the vent 3 is provided on the pressing portion 23; in another embodiment, the vent 3 is provided on the sealing chamber section 22; in another embodiment, the vent 3 is provided on the mounting section 24.

[0058] When the diameter of the nanospray needle 1 is small, a force is applied to the pressing portion 23 to compress the negative pressure chamber 21 , and the vent 3 is opened. The vent 3 can quickly discharge the gas in the negative pressure chamber 21 , thereby accelerating the compression speed of the negative pressure chamber 21 .

[0059] At the same time, since the vent 3 is provided on the pressing part 23, the vent 3 can be conveniently blocked when the pressing part 23 returns to its original state, so that negative pressure is generated in the negative pressure chamber 21, and the nanospray needle 1 can absorb the sample solution. When the vent 3 is released, the external gas can quickly enter the negative pressure chamber 21 from the vent hole, so that the negative pressure in the negative pressure chamber 21 will not act on the nanospray needle 1, so that the nanospray needle 1 absorbs the sample solution, which is convenient for stopping the absorption of the sample solution at any time, and can also prevent the sample solution from penetrating too deeply into the nanospray needle 1, so that the sample solution is convenient for spraying out from the tip of the nanospray needle 1.

[0060] Reference Figure 2 and Figure 3 In this embodiment, the pressing portion 23 is provided with one, and a docking surface 27 for abutting against the mass spectrometer is provided on the side of the sealed cavity section 22 away from the pressing portion 23. The conductive component 5 includes an electrode 51 for connecting to a power source and a conductive structure 52 electrically connected to the electrode 51. The electrode 51 is provided on the docking surface 27. After the sealed cavity section 22 is provided on the mass spectrometer, the docking surface 27 cooperates with the mass spectrometer, and can facilitate the electrical connection between the electrode 51 and the power source on the mass spectrometer.

[0061] The relative position of the conductive structure 52 and the nanospray needle 1 is set as needed. The conductive structure 52 is set inside the nanospray needle 1, and there is a distance between the conductive structure 52 and the nanospray needle 1; or the conductive structure 52 is set inside the nanospray needle 1, and the conductive structure 52 abuts against the inner wall of the nanospray needle 1; or the conductive structure 52 is set outside the nanospray needle 1, and the conductive structure 52 abuts against the outer wall of the nanospray needle 1; or the conductive structure 52 is set outside the nanospray needle 1, and there is a distance between the conductive structure 52 and the nanospray needle 1; or the nanospray needle 1 itself constitutes the conductive structure 52, and the nanospray needle 1 is made of conductive material.

[0062] The conductive structure 52 is made of conductive materials, including metals, alloys, composite metals, conductive plastics, conductive rubbers, conductive fibers, conductive coatings, conductive adhesives, conductive films, polymer semiconductors, polymer metals, polymer superconductors, conductive non-metallic materials, ionic liquids, and conductive liquids, either alone or in combination. The shape of the conductive structure 52 is set as required, including single or in combination structures of wires, sheets, loops, tubes, films, needles, floccules, powders, three-dimensional blocks, nanostructures, and liquids.

[0063] In this embodiment, the conductive structure 52 is a conductive wire, one end of which is electrically connected to the electrode 51. The conductive wire is away from the end of the electrode 51, passes through the negative pressure chamber 21, and is inserted into the nanospray needle 1 from the rear end of the nanospray needle 1, and penetrates to a position close to the tip of the nanospray needle 1. After the nanospray needle 1 absorbs the sample solution, it is connected to the power supply through the electrode 51, and the conductive wire generates an electric field covering the sample solution, so that the sample solution is ejected as an electrospray. The conductive wire is inserted into the nanospray needle 1, which can facilitate the replacement of the nanospray needle 1 or the conductive wire separately.

[0064] Reference Figure 1 and Figure 2 A needle tip protection structure 26 is fixedly connected to one end of the mounting section 24 away from the sealing chamber section 22. The needle tip protection structure 26 is arranged around the periphery of the nanospray needle 1. The needle tip protection structure 26 is integrally formed on the mounting section 24. The shape of the needle tip protection structure 26 is set according to needs, including a single support structure and a support array structure, a flat structure, a cylindrical structure, a funnel-shaped structure, a mesh structure, a hollow structure, an auxiliary external shell structure, etc.

[0065] In this embodiment, the needle tip protection structure 26 is four legs, all of which are parallel to the nanospray needle 1 and are distributed around the nanospray needle 1. The legs can protect the nanospray needle 1 and reduce the probability of damage to the nanospray needle 1.

[0066] The working principle of the embodiment is as follows: when the vent 3 is open, a force is applied to the pressing portion 23, the negative pressure chamber 21 is compressed, and the gas in the negative pressure chamber 21 is quickly discharged from the vent 3, the tip of the nanospray needle 1 is immersed in the sample solution, the vent 3 is blocked, and the force applied to the pressing portion 23 is stopped, the pressing portion 23 is restored to its original state, and negative pressure is generated in the negative pressure chamber 21, so that the sample solution can quickly enter the nanospray needle 1, which is convenient for the nanospray needle 1 to simply and quickly take samples.

[0067] Embodiment 2:

[0068] Reference Figure 4 and Figure 5The difference between this embodiment and the embodiment is that an adhesive 6 is provided between the nanospray needle 1 and the needle insertion channel 25, and the nanospray needle 1 is sealed in the needle insertion channel 25 by the adhesive 6. The pressing part 23 is made of latex, and there are two pressing parts 23. The two pressing parts 23 are respectively arranged on the opposite side surfaces of the sealing cavity section 22, and one vent 3 is provided. The docking surface 27 is arranged on the mounting section 24, and the vent 3 and the docking surface 27 are arranged on the opposite sides of the nanospray box 2.

[0069] Reference Figure 5 and Figure 6 The conductive structure 52 is a conductive film, which is coated on the outer wall of the nanospray needle 1. The conductive film extends from the middle of the nanospray needle 1 to the tip. The conductive film is electrically connected to the electrode 51. After the nanospray needle 1 absorbs the sample solution, the electrode 51 is connected to the power supply, and an electric field covering the sample solution is generated through the conductive film, so that the sample solution is ejected as an electrospray. The conductive film and the nanospray needle 1 can be integrated into a whole to prevent the conductive film from affecting the nanospray needle 1 to absorb or eject the sample solution.

[0070] The implementation principle of the embodiment is as follows: when the vent 3 is open, a force is applied to the two pressing parts 23 at the same time, the negative pressure chamber 21 can be quickly compressed, and the gas in the negative pressure chamber 21 is quickly discharged from the vent 3, the tip of the nanospray needle 1 is immersed in the sample solution, the vent 3 is blocked, and the force applied to the two pressing parts 23 is stopped. The two pressing parts 23 return to their original state, and negative pressure is generated in the negative pressure chamber 21, so that the sample solution can quickly enter the nanospray needle 1, which is convenient for the nanospray needle 1 to simply and quickly take samples.

[0071] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A rapid sampling needle device, characterized in that: It comprises a nanospray needle (1) and a nanospray box (2) arranged on the rear end of the nanospray needle (1); A negative pressure chamber (21) is provided in the nanospray box (2) and is connected to the rear end of the nanospray needle (1); the cavity wall of the nanospray box (2) at the negative pressure chamber (21) is made of a deformable and restorable material; The nanospray box (2) is provided with a vent (3) connected to the negative pressure chamber (21); the nanospray box (2) comprises a sealed chamber section (22) for opening the negative pressure chamber (21); a pressing portion (23) for compressing the negative pressure chamber (21) is provided on the sealed chamber section (22); the pressing portion (23) is made of a deformable and restorable material; the vent (3) is provided on the pressing portion (23). The nanospray box (2) further comprises a mounting section (24) for fixing the nanospray needle (1), the mounting section (24) being located at one end of the sealed cavity section (22) close to the nanospray needle (1), a needle insertion channel (25) communicating with the negative pressure cavity (21) being provided on the mounting section (24), the rear end of the nanospray needle (1) being inserted into the needle insertion channel (25); the nanospray needle (1) is sealed and arranged in the needle insertion channel (25); The invention also comprises a conductive component (5), wherein the conductive component (5) comprises an electrode (51) for connecting to a power source and a conductive structure (52) electrically connected to the electrode (51), wherein the conductive structure (52) can generate an electric field covering the sample solution, so that the sample solution forms an electrospray and is ejected from the tip of the nanospray needle (1).

2. A rapid sampling needle device according to claim 1, characterized in that: A mounting seal ring (4) is sleeved on the nanospray needle (1), and the outer wall of the mounting seal ring (4) is fixedly connected to the inner wall of the needle insertion channel (25).

3. A rapid sampling needle device according to claim 2, characterized in that: A needle tip protection structure (26) is arranged on one end of the mounting section (24) away from the sealing chamber section (22), and the needle tip protection structure (26) is arranged around the periphery of the nanospray needle (1).

4. A rapid sampling needle device according to claim 3, characterized in that: The nanospray box (2) is provided with a docking surface (27) for abutting against a mass spectrometer, and the electrode (51) is arranged on the docking surface (27).

5. A rapid sampling needle device according to claim 4, characterized in that: The conductive structure (52) is arranged inside the nanospray needle (1); or the conductive structure (52) is arranged outside the nanospray needle (1); or the nanospray needle (1) itself constitutes the conductive structure (52).

6. A rapid sampling needle device according to claim 5, characterized in that: When the conductive structure (52) is disposed in the nanospray needle (1), the conductive structure (52) is a conductive wire made of a conductive material, and the conductive wire is inserted in the nanospray needle (1); When the conductive structure (52) is arranged outside the nanospray needle (1), the conductive structure (52) is a conductive film made of a conductive material, and the conductive film is coated on the outer wall of the nanospray needle (1); When the nanospray needle (1) itself constitutes the conductive structure (52), the nanospray needle (1) is a nanospray needle (1) made of a conductive material.

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