Method for degrading polycyclic aromatic hydrocarbons in coal gangue and application thereof

Abstract

The application provides a degradation method of polycyclic aromatic hydrocarbons in coal gangue and application thereof, and the degradation method comprises the following steps: mixing coal gangue, microbial strains with biological weathering, microbial strains for degrading polycyclic aromatic hydrocarbons, nutrients and water, and culturing, so that the degradation of polycyclic aromatic hydrocarbons in the coal gangue is completed; the polycyclic aromatic hydrocarbons include any one or a combination of at least two of naphthalene, acetylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benz[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, indeno[1,2,3-c,d]pyrene, benz[a,h]anthracene and benzo[g,h,i]perylene. The degradation method of polycyclic aromatic hydrocarbons in the coal gangue provided by the application can simultaneously degrade multiple polycyclic aromatic hydrocarbons, and has good degradation effect.

Description

[0001] This application claims priority to Chinese Patent Application No. 202111208675.1, filed on October 18, 2021, entitled "A method for degrading polycyclic aromatic hydrocarbons in coal gangue and its application", the entire contents of which are incorporated herein by reference. Technical Field

[0002] This invention belongs to the field of solid waste treatment, specifically relating to a method for degrading polycyclic aromatic hydrocarbons (PAHs) in coal gangue and its application, and particularly to a method for degrading PAHs in coal gangue with good degradation effect and its application. Background Technology

[0003] Coal gangue is a major type of solid waste. Due to insufficient effective utilization, with a utilization rate of only about 50%, it has resulted in large-scale stockpiling. Currently, apart from a small portion used for brick making, cement calcination, power generation, road paving, and backfilling, most coal gangue is stored in the open, posing serious ecological and environmental safety hazards. The long-term stockpiling of large quantities of coal gangue not only occupies vast amounts of land and triggers landslides, creating significant safety risks for surrounding residents, but also causes a series of physicochemical changes, leading to the release of toxic and harmful substances. These substances seep into the surrounding soil and water, harming the ecological environment and human health. Therefore, strengthening the resource utilization of coal gangue is urgently needed.

[0004] In addition to beneficial organic matter, coal gangue also contains harmful organic substances such as polycyclic aromatic hydrocarbons (PAHs), which is the primary problem to be solved in the ecological utilization of coal gangue. PAHs are a class of chemically stable organic pollutants that can not only delay seed germination and slow root growth, reducing aboveground biomass, chlorophyll content, and root peroxidase activity, and decreasing microbial biomass, but also cause skin diseases, leukemia, and lung cancer due to their intermediate metabolites (such as phenols, esters, or aromatic carboxylic acids). They have been listed as priority pollutants. How to achieve the efficient degradation of toxic PAHs in coal gangue is one of the key issues that needs to be addressed in the ecological utilization of coal gangue.

[0005] CN109928836A describes using coal gangue as a raw material for preparing mineral compound fertilizer. The process involves raw material testing, crushing, batching, mixing, molding, thermal activation, quenching, drying, pulverizing, amino acid immobilization, and secondary drying to produce the mineral organic compound fertilizer. This compound fertilizer has adsorption properties, and its mesopores and micropores can provide a carrier for beneficial microorganisms, promoting the decomposition of organic matter and increasing the content of trace elements in the soil. However, before using the coal gangue, the raw material testing only included chemical composition and phase analysis, without determining the harmful organic substances or conducting a risk assessment after application to the soil.

[0006] CN103045507A discloses a method for isolating and screening highly efficient degrading bacterial complexes in polycyclic aromatic hydrocarbon (PAH) sites and its application. The method involves identifying, isolating, and purifying high-molecular-weight PAH-degrading bacteria from soil samples, optimizing their combination, expanding their culture, and then reintroducing them into long-term contaminated soil containing high concentrations of high-molecular-weight PAHs. This is achieved through periodic addition of appropriate amounts of nutrients in different ratios and control of humidity. However, while the bacteria isolated and purified by this patent exhibit high degradation rates for low-molecular-weight PAHs, their degradation rates for high-molecular-weight PAHs are less than ideal.

[0007] Due to the severe pollution caused by coal gangue, particularly the significant environmental impact of polycyclic aromatic hydrocarbons (PAHs), finding an effective method to degrade PAHs in coal gangue has become an urgent problem to be solved. Summary of the Invention

[0008] To address the shortcomings of existing technologies, the present invention aims to provide a method for degrading polycyclic aromatic hydrocarbons (PAHs) in coal gangue and its application, particularly a method for degrading PAHs in coal gangue with good degradation effect and its application. The method for degrading PAHs in coal gangue provided by the present invention can simultaneously degrade multiple PAHs, achieving good degradation results.

[0009] To achieve this objective, the present invention adopts the following technical solution:

[0010] On one hand, the present invention provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue, the degradation method comprising the following steps:

[0011] The degradation of polycyclic aromatic hydrocarbons in coal gangue is completed by mixing coal gangue, microbial strains with biological weathering function, microbial strains that degrade polycyclic aromatic hydrocarbons, nutrients and water, and then culturing them.

[0012] The polycyclic aromatic hydrocarbons include any one or at least two combinations of naphthalene, acenaphthene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, indo[1,2,3-c,d]pyrene, dibenzo[a,h]anthracene, and benzo[g,h,i]perylene, such as combinations of naphthalene and acenaphthene, acenaphthene and fluorene, or anthracene and fluoranthene, but are not limited to the combinations listed above. Other combinations not listed above are also applicable.

[0013] The above degradation method significantly improves the degradation effect of polycyclic aromatic hydrocarbons in coal gangue by combining microbial strains with bioweathering properties and microbial strains that degrade polycyclic aromatic hydrocarbons.

[0014] The culture includes both light-protected and non-light-protected culture.

[0015] Preferably, the mass ratio of the coal gangue to the microbial strains with biological weathering function is 1000:(0.1-0.4).

[0016] Preferably, the water content in the mixed system is 10-60%.

[0017] The mass ratio of coal gangue to microbial strains with biological weathering function can be 1000:0.1, 1000:0.15, 1000:0.2, 1000:0.25, 1000:0.3, 1000:0.35, or 1000:0.4, etc., and the moisture content can be 10%, 20%, 30%, 40%, 50%, or 60%, etc., but is not limited to the values ​​listed above. Other unlisted values ​​within the above range are also applicable.

[0018] Preferably, the microbial strains with bioweathering function include microbial strains of weathered pyrite and / or microbial strains of weathered aluminosilicate minerals.

[0019] The aforementioned specific microbial strains with bioweathering properties can dissociate inorganic minerals, effectively exposing polycyclic aromatic hydrocarbons (PAHs) inside coal gangue. When combined with microbial strains that degrade PAHs, they work synergistically to improve the degradation effect of PAHs. Furthermore, the combination of microbial strains for weathered pyrite and microbial strains for weathered aluminosilicate minerals further enhances the effect of exposing PAHs.

[0020] Preferably, the microbial strains of the weathered pyrite include desulfurizing bacteria.

[0021] Preferably, the desulfurizing bacteria include any one or a combination of at least two of the following: Thiobacillus species, Thiorhynchus species, and Sulfophyllum species. For example, a combination of Thiobacillus and Thiorhynchus species, a combination of Thiobacillus and Sulfophyllum species, or a combination of Thiorhynchus and Sulfophyllum species, etc., but not limited to the combinations listed above. Other combinations not listed above are also applicable.

[0022] Preferably, the microbial strains of the weathered aluminosilicate minerals include silicate bacteria.

[0023] Preferably, the silicate bacteria include Bacillus mucilaginosus and / or Bacillus amyloliquefaciens.

[0024] Preferably, the mass ratio of the coal gangue to the microbial strains that degrade polycyclic aromatic hydrocarbons is 1000:(0.1-0.6), such as 1000:0.1, 1000:0.2, 1000:0.3, 1000:0.4, 1000:0.5 or 1000:0.6, but is not limited to the values ​​listed above. Other unlisted values ​​within the above range are also applicable.

[0025] Preferably, the microbial strains for degrading polycyclic aromatic hydrocarbons include any one or a combination of at least two of bacteria, fungi, or pre-screened strains, such as a combination of bacteria and fungi, a combination of bacteria and pre-screened strains, or a combination of fungi and pre-screened strains, but are not limited to the combinations listed above. Other combinations not listed above are also applicable, with a combination of bacteria and fungi being preferred.

[0026] The selection of the above-mentioned specific combinations of microbial strains for degrading polycyclic aromatic hydrocarbons can improve the degradation effect of the microbial strains on polycyclic aromatic hydrocarbons in coal gangue.

[0027] Preferably, the bacteria include any one or a combination of at least two of Bacillus megaterium, Mycobacterium, Rhodococcus, or Pseudomonas, such as a combination of Bacillus megaterium and Mycobacterium, a combination of Mycobacterium and Rhodococcus, or a combination of Bacillus megaterium and Pseudomonas, but are not limited to the combinations listed above. Other combinations not listed above are also applicable.

[0028] Preferably, the fungus includes any one or a combination of at least two of white-rot fungi, Aspergillus flavus, Trichoderma echinosporum, or Trichoderma harzianum.

[0029] Preferably, the pre-screened bacterial strain is prepared by a method comprising the following steps:

[0030] (1) Mix coal gangue with water and culture to obtain bacterial solution;

[0031] (2) Spread the bacterial solution obtained in step (1) onto a culture medium containing polycyclic aromatic hydrocarbons and observe the morphological characteristics of the colonies. Inoculate the grown colonies into a new culture medium containing polycyclic aromatic hydrocarbons. Repeat the above steps until only a single colony exists on the culture medium, thus obtaining the pre-screened bacterial strain.

[0032] Preferably, the microbial strains that degrade polycyclic aromatic hydrocarbons are a combination of Bacillus megaterium, Trichoderma echinosporum, and Trichoderma harzianum.

[0033] The selection of the above-mentioned specific combinations of microbial strains for degrading polycyclic aromatic hydrocarbons can further improve the degradation effect of the microbial strains on polycyclic aromatic hydrocarbons in coal gangue.

[0034] Preferably, the nutrients include nitrogen fertilizer and / or phosphorus fertilizer.

[0035] Preferably, in the mixed system, the mass ratio of carbon, nitrogen, and phosphorus is (60-300):(7-10):1, wherein the number of carbon parts can be 60, 80, 100, 120, 140, 160, 180, 200, 220, 240, 260, 280, or 300, etc., and the number of nitrogen parts can be 7, 7.5, 8, 8.5, 9, 9.5, or 10, etc., but is not limited to the values ​​listed above. Other unlisted values ​​within the above range are also applicable.

[0036] The addition of the above nutrients can meet the needs of the microbial strain during the degradation process, enabling it to complete the degradation of polycyclic aromatic hydrocarbons.

[0037] Preferably, the coal gangue is further crushed and sieved before mixing.

[0038] Preferably, the sieve mesh size is 20-200 mesh.

[0039] Preferably, the culture time is at least 30 days.

[0040] Preferably, the culture temperature is 20-40℃.

[0041] Preferably, the culture process requires turning the plants over once every 15-30 days.

[0042] The mesh size for sieving can be 20, 40, 60, 80, 100, 120, 140, 160, 180, or 200 mesh, etc. The cultivation time can be 30, 40, 50, or 60 days, etc. The temperature can be 20℃, 25℃, 30℃, 35℃, or 40℃, etc. The frequency of turning can be once every 15 days, once every 16 days, once every 17 days, once every 18 days, once every 19 days, once every 20 days, once every 21 days, once every 22 days, once every 23 days, once every 24 days, once every 25 days, once every 26 days, once every 27 days, once every 28 days, once every 29 days, or once every 30 days, etc., but is not limited to the values ​​listed above. Other unlisted values ​​within the above range are also applicable.

[0043] On the other hand, the present invention also provides the application of the method for degrading polycyclic aromatic hydrocarbons in coal gangue as described above in solid waste treatment.

[0044] Compared with the prior art, the present invention has the following beneficial effects:

[0045] This invention provides a method for degrading polycyclic aromatic hydrocarbons (PAHs) in coal gangue. By employing a combination of microbial strains with bioweathering capabilities and those specifically designed to degrade PAHs, the method achieves a synergistic effect, significantly improving the degradation efficiency of PAHs in coal gangue. Selecting specific microbial strains with bioweathering capabilities can dissociate inorganic minerals, effectively exposing PAHs within the coal gangue. The combination of these microbial strains with PAH-degrading microbial strains further enhances the degradation effect. The use of a combination of microbial strains derived from weathered pyrite and weathered aluminosilicate minerals further improves the exposure of PAHs. Simultaneously, the selection of specific combinations of PAH-degrading microbial strains enhances the degradation efficiency of PAHs in coal gangue. Detailed Implementation

[0046] To further illustrate the technical means and effects of the present invention, the following describes the technical solution of the present invention in conjunction with preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0047] In the following examples, the coal gangue comes from Yulin;

[0048] Bacillus megaterium was purchased from Beina Biotechnology, model number BNCC-336739;

[0049] Trichoderma harzianum was purchased from Beina Biotechnology, model number BNCC-336568;

[0050] Trichoderma echinosporum was purchased from Beina Biotechnology, model number BNCC-227595;

[0051] The mycobacteria were purchased from Beina Biotechnology, model number BNCC-104221;

[0052] The white-rot fungus was purchased from Beina Biotechnology, model number BNCC-336257;

[0053] The sulfur leaf fungus was purchased from Beina Biotechnology, model number BNCC164799;

[0054] Thiobacillus and Thiorhynchus were purchased from Beina Biotechnology, model number BNCC340818.

[0055] Bacillus mucilaginosus was purchased from Beina Biotechnology, model number BNCC122448;

[0056] Bacillus amyloliquefaciens was purchased from Beina Biotechnology, model number BNCC188070.

[0057] Preparation Example 1

[0058] This preparation example provides a pre-screening strain, prepared by the following method:

[0059] (1) Mix coal gangue with water (solid-liquid ratio 1kg:10L), and culture at 30℃ and 200r / min for 10 days to obtain bacterial culture;

[0060] (2) The bacterial culture obtained in step (1) was spread onto a culture medium containing polycyclic aromatic hydrocarbons (naphthalene, acenaphthene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, indo[1,2,3-c,d]pyrene, dibenzo[a,h]anthracene, and benzo[g,h,i]perylene, 200 mg / L each) (culture medium components: KH2PO4 0.5 g, K2HPO4 0.5 g, (NH4)2SO4 2.5 g, Na2SO4 1.0 g, CaCl2 0.1 g, MgSO4 1.0 g, yeast extract 0.1 g, sodium lactate 1.0 mL, NaCl The culture medium (30g, 20g agar, 1000mL distilled water) was incubated at 30℃ for 5 days. The morphological characteristics of the colonies were observed. The colonies that grew were then inoculated into a new medium containing polycyclic aromatic hydrocarbons and cultured. The above steps were repeated until only a single colony remained on the medium, thus obtaining the pre-screened strain.

[0061] In the following examples, all pre-screened bacterial strains used were derived from Preparation Example 1.

[0062] Example 1

[0063] This embodiment provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue, and the specific steps are as follows:

[0064] (1) Crush the coal gangue through a 100-mesh sieve, take 1000 parts by weight of the sieved coal gangue, add 0.1 parts by weight of Bacillus megaterium, 0.1 parts by weight of Trichoderma acicularis, 0.1 parts by weight of Trichoderma harzianum, 0.1 parts by weight of Sulfidea spp., and 0.15 parts by weight of Bacillus mucilaginosus, and mix them evenly.

[0065] (2) Adjust the nutrient conditions of the mixture in step (1) by adding nitrogen fertilizer and phosphorus fertilizer, and adding water to make the C:N:P ratio 150:8.5:1 and the water content 30%.

[0066] (3) Non-light-protected culture was carried out at 30℃ to degrade polycyclic aromatic hydrocarbons in coal gangue for 30 days.

[0067] Example 2

[0068] This embodiment provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue, and the specific steps are as follows:

[0069] (1) Crush the coal gangue through a 20-mesh sieve, take 1000 parts by weight of the sieved coal gangue, add 0.1 parts by weight of Bacillus megaterium, 0.2 parts by weight of Thiophanate, and 0.2 parts by weight of Bacillus amyloliquefaciens, and mix them evenly.

[0070] (2) Adjust the nutrient conditions of the mixture in step (1) by adding nitrogen fertilizer and phosphorus fertilizer, and adding water to make the C:N:P ratio 60:7:1 and the water content 10%.

[0071] (3) The polycyclic aromatic hydrocarbons of coal gangue were degraded for 30 days by incubating in the dark at 20℃.

[0072] Example 3

[0073] This embodiment provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue, and the specific steps are as follows:

[0074] (1) Crush the coal gangue through a 20-mesh sieve, take 1000 parts by weight of the sieved coal gangue, add 0.6 parts by weight of the pre-screened bacterial strain and 0.1 parts by weight of Thiobacillus, and mix evenly.

[0075] (2) Adjust the nutrient conditions of the mixture in step (1) by adding nitrogen fertilizer and phosphorus fertilizer, and adding water to make the C:N:P ratio 300:10:1 and the water content 60%.

[0076] (3) The polycyclic aromatic hydrocarbons of coal gangue were degraded for 30 days by incubating in the dark at 40℃.

[0077] Example 4

[0078] This embodiment provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue. The specific steps are the same as in Example 1, except that 0.1 parts by weight of Bacillus megaterium, 0.1 parts by weight of Trichoderma acicularis, and 0.1 parts by weight of Trichoderma harzianum in step (1) are replaced with 0.15 parts by weight of Bacillus megaterium and 0.15 parts by weight of Mycobacterium.

[0079] Example 5

[0080] This embodiment provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue. In the specific steps, except that 0.1 parts by weight of Bacillus megaterium, 0.1 parts by weight of Trichoderma harzianum, and 0.1 parts by weight of Trichoderma harzianum in step (1) are replaced with 0.15 parts by weight of white rot fungi and 0.15 parts by weight of Trichoderma harzianum, the rest are the same as in Example 1.

[0081] Example 6

[0082] This embodiment provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue. The specific steps are the same as in Example 1, except that 0.1 parts by weight of Bacillus megaterium, 0.1 parts by weight of Trichoderma echinosporum, and 0.1 parts by weight of Trichoderma harzianum in step (1) are replaced with 0.3 parts by weight of Bacillus megaterium.

[0083] Example 7

[0084] This embodiment provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue. The specific steps are the same as in Example 1, except that 0.1 parts by weight of Bacillus megaterium, 0.1 parts by weight of Trichoderma echinosporum, and 0.1 parts by weight of Trichoderma harzianum are replaced with 0.3 parts by weight of Trichoderma harzianum in step (1).

[0085] Example 8

[0086] This embodiment provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue. Except for the degradation time of 60 days in step (3), the specific steps are the same as in embodiment 1.

[0087] Example 9

[0088] This embodiment provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue. The specific steps are the same as in Example 1, except that it does not contain sulfur-containing leaf bacteria and reduces the amount allocated to Bacillus mucilaginosus.

[0089] Example 10

[0090] This embodiment provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue. The specific steps are the same as in Example 1, except that it does not contain Bacillus mucilaginosus and reduces the amount allocated to sulfur-containing bacteria.

[0091] Comparative Example 1

[0092] This comparative example provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue. The specific steps are the same as in Example 1, except that step (1) does not include sulfur leaf bacteria and mucilage bacillus, and the reduced portion is allocated proportionally to Bacillus megaterium, Trichoderma acicularis and Trichoderma harzianum.

[0093] Comparative Example 2

[0094] This comparative example provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue. The specific steps are the same as in Example 1, except that step (1) does not include Bacillus megaterium, Trichoderma acicularis, and Trichoderma harzianum, and the reduced portion is allocated proportionally to Bacillus sulfide and Bacillus mucilaginosus.

[0095] Comparative Example 3

[0096] This comparative example provides a method for degrading polycyclic aromatic hydrocarbons in coal gangue, and the specific steps are as follows:

[0097] (1) Crush the coal gangue through a 100-mesh sieve, take 1000 parts by weight of the sieved coal gangue, add 0.1 parts by weight of Bacillus megaterium, 0.1 parts by weight of Trichoderma acicularis, 0.1 parts by weight of Trichoderma harzianum, 0.1 parts by weight of Sulfidea spp., and 0.15 parts by weight of Bacillus mucilaginosus, and mix them evenly.

[0098] (2) Add water to the mixture in step (1) with a water content of 30%;

[0099] (3) The polycyclic aromatic hydrocarbons of coal gangue were degraded for 30 days by incubating in the dark at 30℃.

[0100] Degradation performance test:

[0101] Polycyclic aromatic hydrocarbons (PAHs) in the degraded samples from Examples 1-10 and Comparative Examples 1-3, as well as the blank control (original coal gangue sample), were detected by HPLC (C18 4.6 mm × 250 mm × 5 μm). The results are as follows:

[0102]

[0103]

[0104] The above results show that the degradation method provided by this invention has excellent degradation effect on polycyclic aromatic hydrocarbons (PAHs) in coal gangue. Comparing Examples 1 and 4, 5, it can be found that this invention further improves the degradation effect on PAHs by using a combination of preferred bacteria and fungi. Comparing Examples 1 and 4-7, it can be found that the degradation effect is further improved within the range of the preferred microbial strain combinations of this invention. Comparing Examples 1 and 8, it can be found that the degradation effect can be improved by appropriately extending the degradation time. Comparing Examples 1 and 9-10, it can be found that this invention, by using a combination of microbial strains from weathered pyrite and weathered aluminosilicate minerals, achieves a synergistic effect, further improving the exposure effect on PAHs and thus improving the degradation effect on PAHs. Comparing Examples 1 and Comparative Examples 1-2, it can be found that this invention, by using a combination of microbial strains with bio-weathering properties and microbial strains that degrade PAHs, significantly improves the degradation effect on PAHs. Comparing Examples 1 and Comparative Example 3, it can be found that this invention improves the degradation effect of the strains on PAHs by adding nutrients.

[0105] The applicant declares that this invention illustrates the method for degrading polycyclic aromatic hydrocarbons in coal gangue and its application through the above embodiments. However, this invention is not limited to the above embodiments, meaning that this invention does not necessarily rely on the above embodiments for implementation. Those skilled in the art should understand that any improvements to this invention, equivalent substitutions of raw materials for the product, addition of auxiliary components, and selection of specific methods all fall within the protection and disclosure scope of this invention.

[0106] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details of the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0107] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable way without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A method for degrading polycyclic aromatic hydrocarbons in coal gangue, characterized in that, The degradation method includes the following steps: The degradation of polycyclic aromatic hydrocarbons in coal gangue is completed by mixing coal gangue, microbial strains with biological weathering function, microbial strains that degrade polycyclic aromatic hydrocarbons, nutrients and water, and then culturing them. The microbial strains with bioweathering function are a combination of *Sulphurella* strains of weathered pyrite and *Bacillus mucilaginosus* strains of weathered aluminosilicate minerals; the microbial strains with bioweathering function are used to dissociate inorganic minerals, thereby exposing polycyclic aromatic hydrocarbons inside the coal gangue; the mass ratio of coal gangue to microbial strains with bioweathering function is 1000:(0.1-0.4); The microbial strain for degrading polycyclic aromatic hydrocarbons is a combination of the bacteria Bacillus megaterium, the fungi Trichoderma echinosporum, and the fungi Trichoderma harzianum; the mass ratio of the coal gangue to the microbial strain for degrading polycyclic aromatic hydrocarbons is 1000:(0.1-0.6); The coal gangue is crushed and sieved before mixing, and the sieve mesh size is 20-200 mesh. The polycyclic aromatic hydrocarbons include any one or a combination of at least two of the following: naphthalene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, indo[1,2,3-c,d]pyrene, dibenzo[a,h]anthracene, and benzo[g,h,i]perylene.

2. The method for degrading polycyclic aromatic hydrocarbons in coal gangue according to claim 1, characterized in that, The water content in the mixed system is 10-60%.

3. The method for degrading polycyclic aromatic hydrocarbons in coal gangue according to claim 1, characterized in that, The nutrients include nitrogen fertilizer and / or phosphorus fertilizer.

4. The method for degrading polycyclic aromatic hydrocarbons in coal gangue according to claim 3, characterized in that, In the mixed system, the mass ratio of carbon, nitrogen and phosphorus is (60-300):(7-10):

1.

5. The method for degrading polycyclic aromatic hydrocarbons in coal gangue according to claim 1, characterized in that, The culture period is at least 30 days.

6. The method for degrading polycyclic aromatic hydrocarbons in coal gangue according to claim 1, characterized in that, The culture temperature is 20-40℃.

7. The method for degrading polycyclic aromatic hydrocarbons in coal gangue according to claim 1, characterized in that, The culture process requires turning the plants over, and the turning frequency is once every 15-30 days.

8. The application of a method for degrading polycyclic aromatic hydrocarbons in coal gangue according to any one of claims 1-7 in solid waste treatment.

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