Application of soluble sophora japonica powder in preparing weight loss products

Through enzymatic treatment and spray drying technology, soluble sophora flour with high solubility and high flavonoid content was prepared, which solved the problem of low solubility of sophora and achieved its application and application scope in weight loss and fat loss products.

CN115997928BActive Publication Date: 2024-12-06FOSHAN GOLDEN HEALTH TECH CO LTD

Patent Information

Application Number
CN202211555737.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-12-06
Publication Date
2024-12-06
Estimated Expiration
2042-12-06

AI Technical Summary

Technical Problem

The solubility of sophora itself is very low, which limits its application in beverages, oral liquids, solid beverages, etc., and cannot meet the requirements of products with high solubility performance.

Method used

Treatment of sophora glutinous rice with high solubility and high flavonoid content by enzymatic lysis, including heating extraction, enzymatic lysis reaction, enzyme decomposition, solid-liquid separation and spray drying, soluble sophora glutinous rice powder was prepared.

Benefits of technology

It improves the solubility and flavonoid content of sophora, realizes the application of sophora in weight loss and fat loss products, expands its application range, and significantly reduces obesity symptoms in mouse experiments.

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Abstract

The invention discloses an application of soluble locust bean powder in the preparation of weight loss products. The soluble locust bean powder is obtained by enzymolysis of locust bean; the soluble locust bean powder is directly or indirectly used to prepare products with the functions of controlling weight and alleviating obesity symptoms. The preparation method thereof comprises the following steps: heating extraction, enzymolysis reaction, enzyme inactivation and drying to obtain the soluble locust bean powder. The soluble locust bean powder prepared by the invention has good solubility and higher flavonoid content, and can be widely used in various fields, especially in beverages with high solubility requirements. At the same time, the soluble locust bean powder prepared by the invention has a fat-reducing function, and expands the application field of locust bean in weight loss products.
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Description

Technical Field

[0001] The invention relates to the field of biotechnology, and in particular to application of soluble locust bean powder in preparing weight-loss products. Background Art

[0002] Sophora japonica is the flower bud of the leguminous plant Sophora japonica, which contains selenium, protein and a variety of amino acids required by the human body. Sophora japonica is rich in selenium, which can effectively promote the body's metabolism, improve the body's immunity, and effectively reduce the phenomenon of hair loss. At the same time, Sophora japonica also has antibacterial and anti-inflammatory effects, can effectively kill bacteria, and has a strong therapeutic effect on otitis media, dermatitis, eczema, skin itching, allergic dermatitis, pharyngitis, urethritis, etc. Sophora japonica is rich in nutrients, can effectively moisturize the skin, has the effect of beauty and beauty, can also promote the body's digestion and absorption, and effectively prevent constipation.

[0003] Flavonoids are the main active ingredients of Sophora japonica, which can not only reduce capillary permeability and fragility, but also reduce human blood lipids and cholesterol, promote cell proliferation and prevent blood cell aggregation. In addition, flavonoids also have anti-inflammatory, antiviral, diuretic, antispasmodic, antitussive and anti-radiation effects. They are clinically used to prevent and treat hypertension, cerebral hemorrhage, retinal hemorrhage, purpura and acute hemorrhagic nephritis, and are also used to prevent and treat diabetes and combined hyperlipidemia. But the solubility of Sophora japonica itself is very low. Traditional Sophora japonica is extracted by alkali solution, and the flavonoids in it are taken out, but the solubility is still very poor, which limits the application range of Sophora japonica, such as beverages, oral liquids, solid beverages, and jelly. The present invention overcomes the shortcomings and disadvantages of existing production technology, and provides a gentle, environmentally friendly, easy-to-operate preparation method of soluble Sophora japonica. Simultaneously, the prepared soluble Sophora japonica is evaluated for weight loss and fat reduction efficacy, and a new application field of Sophora japonica is developed.

[0004] In view of this, it is indeed necessary to provide a technical solution to the above-mentioned technical problems. Summary of the invention

[0005] In order to overcome the deficiencies of the prior art, the object of the present invention is to provide an application of a soluble sophora bean powder in the preparation of a weight loss product. The soluble sophora bean powder of the present invention has a fat-reducing function and opens up a new application field of the sophora bean. At the same time, the present invention also provides a preparation method of the above-mentioned soluble sophora bean powder. The raw materials of the preparation method are simple and easy to obtain, the process is simple, and it is suitable for large-scale production. The soluble sophora bean powder prepared by the present invention has good solubility and can be widely used in various fields, especially in products with high solubility requirements.

[0006] The purpose of the present invention can be achieved by adopting the following technical solutions:

[0007] The invention discloses an application of soluble sophora japonica bean powder in preparing weight-loss products. The soluble sophora japonica bean powder is obtained by enzymolysis of the sophora japonica bean. The soluble sophora japonica bean powder is directly or indirectly used for preparing products capable of controlling body weight and alleviating obesity symptoms.

[0008] Preferably, the method for preparing the soluble sophora japonica rice powder comprises the following steps:

[0009] S1, adding water to the buds of Sophora japonica for heating and extraction to obtain a buds of Sophora japonica extract;

[0010] S2, cooling the Sophora japonica extract of step S1, adjusting the pH and adding enzymes for enzymolysis to obtain soluble Sophora japonica extract;

[0011] S3, inactivating the enzyme in the soluble Sophora japonica bud described in step S2, and then collecting the liquid for solid-liquid separation and concentrating to obtain a soluble Sophora japonica bud concentrated solution;

[0012] S4, spray-drying the soluble Sophora japonica leaf concentrate in step S3 to obtain the soluble Sophora japonica leaf powder.

[0013] Preferably, in step S1, the mass ratio of Sophora japonica buds to water is 1:(10-30).

[0014] Preferably, in step S1, the conditions for adding water to the Sophora japonica buds for heating extraction are that the extraction temperature is 80-100° C. and the extraction time is 20-40 min.

[0015] Preferably, the mass ratio of Sophora japonica seeds to enzyme in step S2 is 200:(1-4).

[0016] Preferably, the enzyme added in step S2 is one or a combination of two or more of rhamnosidase, hesperidinase, naringinase, cellulase or pectinase.

[0017] Preferably, in step S2, the Sophora japonica flower extract of step S1 is cooled to a temperature of 40-60° C., the pH is adjusted to 5-6, and the reaction time is 4-6 hours.

[0018] Preferably, in step S3, the conditions for inactivating the enzyme in the soluble Sophora japonica buds described in step S2 are as follows: the inactivation temperature is 80-90° C. and the inactivation time is 20-40 min.

[0019] Preferably, the solid-liquid separation method in step S3 is to first filter through a plate and frame to collect the filtrate, and then centrifuge the filtrate to collect the upper liquid; the concentration condition is to reach a sugar content of 5-20° Bx.

[0020] Preferably, the spray drying conditions in step S4 are as follows: an inlet temperature of 170-190° C., an outlet temperature of 40-60° C., a frequency of 330-350 Hz, and a feed speed of 15-20 r / min.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. The soluble sophora bean powder of the present invention has good solubility and a higher flavonoid content. The soluble sophora bean powder prepared in the present application can reduce the secretion of inflammatory factors in fat, has a fat-reducing function, and alleviates obesity symptoms. It can be directly or indirectly used to prepare products that have the functions of controlling weight and alleviating obesity symptoms, and open up new application fields for sophora bean powder.

[0023] 2. The preparation method of the soluble sophora bean powder of the present invention has simple and readily available raw materials, simple process, and is suitable for large-scale production. The soluble sophora bean powder of the present invention can be widely used in various fields, especially in products with high solubility requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The weight changes of mice in each group in the present invention;

[0025] Figure 2 Schematic diagram of white fat and brown fat of each group of mice in the present invention;

[0026] Figure 3 is a bar graph of the weight of white fat in the epididymis of each group of mice in the present invention;

[0027] Figure 4 is a bar graph of the brown fat weight in the scapular region of each group of mice in the present invention;

[0028] Figure 5 A bar graph showing the expression levels of proteins regulating fat metabolism in white fat of each group of mice in the present invention;

[0029] Figure 6 A bar graph showing the expression levels of proteins regulating fat metabolism in brown fat of each group of mice in the present invention;

[0030] Figure 7 It is a bar graph of the expression level of adipokines in white fat of each group of mice in the present invention;

[0031] Figure 8 It is a bar graph showing the expression levels of adipose factors in brown fat of each group of mice in the present invention. DETAILED DESCRIPTION

[0032] The technical solution of the present invention will be clearly and completely described below in conjunction with specific embodiments. Obviously, the described embodiments are only partial embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0033] The invention discloses an application of soluble sophora japonica bean powder in preparing weight-loss products. The soluble sophora japonica bean powder is obtained by enzymolysis of the sophora japonica bean. The soluble sophora japonica bean powder is directly or indirectly used for preparing products capable of controlling body weight and alleviating obesity symptoms.

[0034] In one embodiment, the soluble sophora bean powder is used to prepare foods, medicines or health products for reducing weight, reducing fat and controlling body weight.

[0035] Sophora japonica is the flower bud of the leguminous plant Sophora japonica, which contains selenium, protein and a variety of amino acids required by the human body, and flavonoids are the main components of Sophora japonica. The solubility of Sophora japonica itself is very low. Traditional Sophora japonica is extracted by alkali solution to extract the flavonoids inside, but the solubility is still very poor, which limits the application range of Sophora japonica and cannot be applied to products with high solubility requirements. Therefore, the present invention provides a method for preparing a soluble Sophora japonica powder to solve the above problems, and the soluble Sophora japonica powder prepared by the present invention has a weight loss effect, a higher flavonoid content, and expands the application range of Sophora japonica, and the soluble Sophora japonica powder can be used as a raw material for food or medicine. The soluble Sophora japonica powder of the present invention has good compatibility and can be used in combination with other components with fat reduction effects.

[0036] In one embodiment, the method for preparing the soluble sophora japonica rice powder comprises the following steps:

[0037] S1, adding water to the buds of Sophora japonica for heating and extraction to obtain a buds of Sophora japonica extract;

[0038] S2, cooling the Sophora japonica extract of step S1, adjusting the pH and adding enzymes for enzymolysis to obtain soluble Sophora japonica extract;

[0039] S3, inactivating the enzyme in the soluble Sophora japonica bud described in step S2, and then collecting the liquid for solid-liquid separation and concentrating to obtain a soluble Sophora japonica bud concentrated solution;

[0040] S4, spray-drying the soluble Sophora japonica leaf concentrate in step S3 to obtain the soluble Sophora japonica leaf powder.

[0041] The soluble sophora japonica powder is obtained by heating and extracting the sophora japonica seeds, adding enzymes for reaction, killing the enzymes, separating and concentrating, and spray drying. The preparation process of the invention is simple, the raw materials are easy to obtain, no harsh process conditions are required, and the powder is suitable for large-scale production. At the same time, the preparation method of the invention well retains the effective components of the sophora japonica seeds, and the flavonoids content is higher.

[0042] In one embodiment, the mass ratio of the sophora japonica buds to water in step S1 is 1:(10-30). Preferably, the mass ratio of the sophora japonica buds to water in step S1 is 1:20.

[0043] In one embodiment, the conditions for adding water to the Sophora japonica seeds for heating extraction in step S1 are that the extraction temperature is 80-100°C and the extraction time is 20-40 minutes. Preferably, the heating extraction temperature is 90°C and the extraction time is 30 minutes.

[0044] During the heating extraction, the mass ratio of Sophora japonica seed to water is 1: (10-30), which can effectively extract the active ingredients in Sophora japonica seed. Temperature can increase the solubility of Sophora japonica seed in water. Therefore, when the extraction temperature is 80-100℃ and the extraction time is 20-40min, the solubility of Sophora japonica seed is better, the extraction efficiency is higher, and the flavor and nutrients of Sophora japonica seed are effectively retained.

[0045] In one embodiment, the mass ratio of Sophora japonica seeds to enzyme in step S2 is 200:(1-4).

[0046] In one embodiment, the enzyme added in step S2 is one or a combination of two or more of rhamnosidase, hesperidinase, naringinase, cellulase or pectinase.

[0047] In one embodiment, in step S2, the Sophora japonica extract of step S1 is cooled to a temperature of 40-60° C., the pH is adjusted to 5-6, and the enzymatic reaction time is 4-6 hours.

[0048] Among them, the enzymatic reaction is carried out in step S2, and the enzymatic reaction must be carried out under mild conditions. Excessive acidity, alkali or high temperature in the system will reduce the activity of the enzyme or directly destroy the structure of the enzyme, and low temperature will reduce the activity of the enzyme. The enzymatic temperature is a condition to increase the activity of the molecule and make the chemical reaction proceed smoothly, and time ensures that the enzymatic reaction is thorough. Therefore, when the temperature is cooled to 40~60℃, the pH value is adjusted to 5~6, and the reaction time is 4~6h, the activity of the enzyme can be effectively maintained, making the enzymatic reaction process more efficient and the enzymatic reaction more thorough. Secondly, the mass ratio of Sophora japonica seed and enzyme in S2 is 200: (1~4). Adding enzyme to the Sophora japonica seed extract obtained after extraction in step S1 can effectively destroy the organizational structure of Sophora japonica seed, fully expose its effective ingredients, improve the solubility of Sophora japonica seed in water, and make the system after the enzymatic reaction clearer.

[0049] In one embodiment, the conditions for inactivating the enzyme in the soluble Sophora japonica seed described in step S2 in step S3 are: the enzyme inactivation temperature is 80-90°C, and the enzyme inactivation time is 20-40 minutes. Preferably, the enzyme inactivation temperature in step S3 is 85°C, and the enzyme inactivation time is 30 minutes.

[0050] The enzyme inactivation temperature in step S3 is 80-90°C and the enzyme inactivation time is 20-40 minutes. The purpose of heating is to coagulate the enzyme so that the enzyme can be separated smoothly and the enzyme is prevented from being added to the product. After heating, the enzyme structure can be directly destroyed due to the high temperature, thereby achieving the purpose of enzyme inactivation.

[0051] In one embodiment, the solid-liquid separation method in step S3 is to first filter through a plate and frame to collect the filtrate, and then centrifuge the filtrate to collect the upper layer; the concentration condition is to reach a sugar content of 5-20° Bx. Preferably, the concentration condition is to reach a sugar content of 10° Bx.

[0052] In one embodiment, the spray drying conditions in step S4 are as follows: the inlet temperature is 170-190°C, the outlet temperature is 40-60°C, the frequency is 330-350Hz, and the feed speed is 15-20 r / min. Preferably, the spray drying conditions in step S4 are as follows: the inlet temperature is 180°C, the outlet temperature is 50°C, the frequency is 340Hz, and the feed speed is 20 r / min.

[0053] Among them, in the spray drying, the inlet temperature is 170~190℃, the outlet temperature is 40~60℃, the frequency is 330~350Hz, and the feed speed is 15-20 r / min. The use of spray drying can make the drying speed fast, the production efficiency high, simplify the production process, and the prepared product has higher purity.

[0054] Below, the preparation method of the soluble sophora japonica flour of the present invention is described with specific examples.

[0055] Example 1

[0056] Take 4 kg of Sophora japonica seeds, add 40 L of water, heat to 80 ° C, and perform heating extraction for 20 min to obtain Sophora japonica seeds extract;

[0057] The extract of Sophora japonica extract was cooled to 40°C, the pH value was adjusted to 5 with citric acid monohydrate, citric acid dodecahydrate and sodium phosphate, 0.02 kg of rhamnosidase was added, stirred and reacted for 6 hours to obtain soluble Sophora japonica extract;

[0058] The soluble Sophora japonica seed is heated to 85°C, the enzyme is inactivated for 20 minutes, the filtrate is separated, the filtrate is collected after plate and frame filtration, the centrifuge is placed in a concentration tank, and vacuum concentrated until the sugar content is 20°Bx, and the concentration is stopped to obtain a soluble Sophora japonica seed concentrate;

[0059] The soluble Sophora japonica seed concentrate was spray-dried to obtain 1.23 kg of soluble Sophora japonica seed powder.

[0060] Example 2

[0061] Take 4 kg of Sophora japonica seeds, add 80 L of water, heat to 90 ° C, and perform heating extraction for 30 minutes to obtain Sophora japonica seeds extract;

[0062] The extract of Sophora japonica extract was cooled to 50°C, the pH value was adjusted to 5.5 with citric acid monohydrate, citric acid dodecahydrate and sodium phosphate, 0.05 kg of hesperidinase was added, stirred and reacted for 5 hours to obtain soluble Sophora japonica extract;

[0063] The soluble Sophora japonica seed is heated to 95°C, the enzyme is inactivated for 30 minutes, the filtrate is separated, the filtrate is collected after plate and frame filtration, the centrifuge is placed in a concentration tank, and vacuum concentrated until the sugar content is 10°Bx, and the concentration is stopped to obtain a soluble Sophora japonica seed concentrate;

[0064] The soluble Sophora japonica seed concentrate was spray-dried to obtain 1.4 kg of soluble Sophora japonica seed powder.

[0065] Example 3

[0066] Take 4 kg of Sophora japonica seeds, add 120 L of water, heat to 100 ° C, and perform heating extraction for 40 minutes to obtain Sophora japonica seeds extract;

[0067] The sophora japonica extract was cooled to 60°C, the pH value was adjusted to 6 with citric acid monohydrate, citric acid dodecahydrate and sodium phosphate, 0.08 kg of naringinase was added, stirred and reacted for 4 hours to obtain soluble sophora japonica;

[0068] The soluble Sophora japonica seed is heated to 90°C, the enzyme is inactivated for 40 minutes, the filtrate is separated, the filtrate is collected after plate and frame filtration, the centrifuge is placed in a concentration tank, and vacuum concentrated until the sugar content is 5°Bx, and the concentration is stopped to obtain a soluble Sophora japonica seed concentrate;

[0069] The soluble Sophora japonica concentrate was spray-dried to obtain 1.36 kg of soluble Sophora japonica powder.

[0070] Example 4

[0071] Take 4 kg of Sophora japonica seeds, add 80 L of water, heat to 90 ° C, and perform heating extraction for 2 hours to obtain Sophora japonica seeds extract;

[0072] The extract of Sophora japonica is separated, and the filtrate is collected after plate and frame filtration for centrifugation, and the centrifuge is placed in a concentration tank for vacuum concentration, and the concentration is stopped when the sugar content reaches 10°Bx to obtain a concentrate of Sophora japonica extract;

[0073] The Sophora japonica extract concentrate was spray-dried to obtain 0.83 kg of Sophora japonica extract.

[0074] Results and Analysis

[0075] 1. Solubility test of soluble sophora japonica powder

[0076] The soluble sophora japonica powder of Examples 1-3 and the sophora japonica extract of Example 4 were prepared into 4%, 8%, 12%, and 16% solutions, respectively, and the solubility properties of the soluble sophora japonica powder of Examples 1-3 and the sophora japonica extract of Example 4 were observed. The solubility test results of the soluble sophora japonica powder of Examples 1-3 and the sophora japonica extract of Example 4 are shown in Table 1.

[0077] Table 1 Solubility test results of soluble Sophora japonica powder and Sophora japonica extract

[0078] Dissolving ratio embodiment 1 embodiment 2 embodiment 3 embodiment 44% clear solution clear solution clear solution has slightly precipitation 8% clear solution clear solution clear solution has more precipitation, solution is turbid 12% clear solution clear solution clear solution has a large amount of precipitation, solution is turbid 16% clear solution clear solution clear solution has a large amount of precipitation, solution is turbid As shown in Table 1, the solubility of the soluble Sophora japonica powder of embodiment 1-3 is better, and solubility can reach 16%, and solution is clear, without precipitation. And the solubility of the Sophora japonica extract of embodiment 4 is poor, and precipitation is produced when Sophora japonica extract content is 4%. Therefore, the solubility performance of the soluble Sophora japonica powder prepared by the present invention is improved, and the application range of Sophora japonica can be better increased, so that it is applied to the product with higher solubility requirement.

[0079] 2. Mouse experiment - test of the weight loss and fat reduction effect of soluble locust bean powder

[0080] 2.1 Grouping and feeding of mice

[0081] The experimental subjects were male C57BL6 mice (6-8 weeks old), a total of 15 mice, and were treated with a 12-hour light-dark cycle at 23.2°C. After a week of adaptation, the mice were randomly divided into 3 groups (n=3, 5 / cage) and fed separately, during which the mice were allowed to get water and food freely, and the mice were maintained on their respective diets for 15 weeks.

[0082] Group 1: normal feed; Group 2: high-fat feed; Group 3: high-fat feed + 0.5% soluble Sophora japonica flour of Example 1.

[0083] Among them, normal feed is commercial mouse feed, with a fat content of 5%, protein of 25%, carbohydrates of 65%, and crude fiber of 5%. High-fat feed contains 20% fat, 0.2% cholesterol, 24.8% protein, 50% carbohydrates (including 35% sucrose) and 5% crude fiber. The contents of each component of the above feed are all in mass percentage.

[0084] 2.2. Experimental observation, testing and recording

[0085] Body weight and food intake were measured weekly, and the appetite, defecation, urine, activity, etc. of the mice were observed daily. The weight of the mice was recorded weekly, and a weight change curve of the mice was drawn. After 15 weeks, the mice were killed and white fat and brown fat were taken from the epididymis and scapular area of ​​the mice, and the weights were weighed. Real-time PCR was used to analyze the expression levels of different fat factors IL-1β, IL-6, IL-10, and TNF-α in the white and brown fat of each group of mice. In the present invention, Figure 1 The weight changes of mice in each group; Figure 2 Schematic diagram of white fat and brown fat in each group of mice; Figure 3 It is a bar graph of the weight of white fat in the epididymis of mice in each group; Figure 4 It is a bar graph of the brown fat weight in the scapular region of each group of mice; Figure 5 The bar graph shows the expression levels of proteins regulating fat metabolism in the white fat of each group of mice; Figure 6 The bar graphs show the expression levels of proteins regulating fat metabolism in brown fat of mice in each group; Figure 7 The bar graphs are the expression levels of adipokines in white fat of mice in each group; Figure 8 The bar graph shows the expression levels of adipokines in brown fat of each group of mice.

[0086] 2.3 Mouse experiment - analysis of the results of the experiment on the weight loss and fat reduction effect of soluble Sophora japonica seeds

[0087] from Figure 1It can be seen that the weight of mice fed with normal feed in Experiment 1 almost maintained a balanced state, the weight of mice fed with high-fat feed in Experiment 2 showed a trend of continuous increase, and the weight gain of mice fed with high-fat feed and 0.5% soluble Sophora japonica powder in Example 1 in Experiment 3 was significantly slowed down. The soluble Sophora japonica powder prepared in the present application has the effect of reducing weight.

[0088] from Figure 2 It can be seen that the mice in Experimental Group 1 fed with normal feed had a smaller white fat volume and a larger brown fat volume, the mice in Experimental Group 2 fed with high-fat feed had the largest white fat volume and the smallest brown fat volume, and the mice in Experimental Group 3 fed with high-fat feed and 0.5% soluble Sophora japonica powder of Example 1 had smaller white fat and larger brown fat. The soluble Sophora japonica powder prepared in this application can reduce the generation of white fat and promote the generation of brown fat.

[0089] In addition, from Figure 3 It can be seen that the weight of white fat in the epididymis of mice fed normal diet in Experiment 1 was the lowest, and the weight of white fat in the epididymis of mice fed high-fat diet and 0.5% soluble locust bean powder of Example 1 in Experiment 3 was significantly lower than that of mice fed high-fat diet in Experiment 2. Figure 4 It can be seen that the weight of brown fat in the shoulder area of ​​mice in Experimental Group 3 fed with high-fat diet and 0.5% soluble Sophora japonica powder of Example 1 was significantly higher than that of mice fed with high-fat diet in Experimental Group 2. The soluble Sophora japonica powder prepared in the present application can reduce the weight of white fat in mice, increase the weight of brown fat, promote the thermal decomposition of fat, and play a role in weight loss.

[0090] from Figure 5 and Figure 6 It can be seen that the mice in Experimental Group 3 that were fed a high-fat diet and 0.5% of the soluble Sophora japonica rice powder of Example 1 had higher expression of proteins that regulate thermogenesis and combustion of white fat and brown fat than those in the high-fat diet group. As shown in the figure, the expression levels of proteins that regulate fat metabolism, such as Ucp1 (uncoupling protein), PGC1α (peroxisome proliferator-activated receptor-γ coactivator-1α), PPARγ2 (peroxisome proliferator-activated receptor-γ), and C / EβPα (enhancer binding protein), which promote fat thermogenesis, were significantly higher in Experimental Group 3 than in Experimental Group 2 that were fed a high-fat diet, indicating that soluble Sophora japonica rice can promote fat thermogenesis and metabolism. Figure 7 and Figure 8It can be seen that the soluble sophora bean powder prepared in the present application can reduce the secretion of inflammatory factors in fat, whether from white fat or brown fat inflammatory factors, the TNF-α, IL-1β, IL-6 and IL-10 of the mice in Experimental Group 3 fed with a high-fat diet and 0.5% soluble sophora bean powder in Example 1 were significantly reduced compared with the mice in Experimental Group 2 fed with a high-fat diet.

[0091] In summary, the soluble sophora japonica bean powder prepared in the present application has a weight loss effect, can well control body weight, can promote the thermal decomposition of fat, and play a role in weight loss.

[0092] For those skilled in the art, various other corresponding changes and deformations can be made according to the technical solutions and concepts described above, and all of these changes and deformations should fall within the protection scope of the claims of the present invention.

Claims

1. Application of soluble Sophora japonica seed powder in the preparation of weight loss products, It is characterized in that The soluble sophora japonica rice powder is obtained by enzymatic hydrolysis of the sophora japonica rice; The preparation method of the soluble sophora japonica rice powder comprises the following steps: S1, adding water to the sophora japonica seeds for heating extraction to obtain a sophora japonica seeds extract; the mass ratio of the sophora japonica seeds to water in step S1 is 1: (10-30); the conditions for adding water to the sophora japonica seeds for heating extraction in step S1 are that the extraction temperature is 80-100°C and the extraction time is 20-40min; S2, cooling the Sophora japonica extract of step S1 to a temperature of 40-60°C, adjusting the pH to 5-6, and performing enzymatic hydrolysis for 4-6 hours to obtain soluble Sophora japonica; the enzyme added in step S2 is rhamnosidase, and the mass ratio of Sophora japonica to enzyme in step S2 is 200:(1-4); S3, inactivating the enzyme in the soluble Sophora japonica bud described in step S2, and then collecting the liquid by solid-liquid separation and vacuum concentrating the liquid to a Brix of 5-20 ° Bx to obtain a soluble Sophora japonica bud concentrated solution; S4, spray-drying the soluble Sophora japonica leaf concentrate in step S3 to obtain the soluble Sophora japonica leaf powder.

2. The use of the soluble Sophora japonica bean powder according to claim 1 in preparing a weight loss product, It is characterized in that In step S3, the conditions for inactivating the enzyme in the soluble Sophora japonica buds described in step S2 are as follows: the inactivation temperature is 80-90° C. and the inactivation time is 20-40 min.

3. The use of the soluble Sophora japonica bean powder according to claim 1 in preparing a weight loss product, It is characterized in that The solid-liquid separation method in step S3 is to first filter through a plate and frame to collect the filtrate, and then centrifuge the filtrate to collect the upper layer liquid.

4. The use of the soluble Sophora japonica flour according to claim 1 in preparing a weight loss product, It is characterized in that The spray drying conditions in step S4 are as follows: inlet temperature is 170-190° C., outlet temperature is 40-60° C., frequency is 330-350 Hz, and feed speed is 15-20 r / min.

Citation Information

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