A method for evaluating the efficacy of tumor reversal drugs

Abstract

The invention discloses a method for evaluating the efficacy of a tumor reversal drug, and belongs to the technical field of tumor drugs. The method includes the step of simulating the hypoxic environment of a tumor with a polydimethylsiloxane membrane. The preparation of the polydimethylsiloxane membrane includes the following steps: S01, adding a catalyst to hydrogen silicone oil and polyethylene glycol methyl ether methacrylate to react; S02, adding vinyl triethoxysilane to react; S03, adding hydroxyl-terminated polydimethylsiloxane and a catalyst to react; S04, doping nanoparticles; S05, coating, degassing, drying, alkaline washing to remove acidic ions, washing with distilled water to neutrality, and drying to obtain the polydimethylsiloxane membrane. The in vitro method for evaluating the efficacy of a tumor reversal drug of the present invention uses a polydimethylsiloxane membrane to simulate the control of the tumor hypoxia microenvironment and effectively evaluate the efficacy of the tumor reversal drug.

Description

Technical Field

[0001] The invention belongs to the technical field of tumor drugs, and particularly relates to a method for evaluating the efficacy of tumor reversal drugs. Background Art

[0002] Tumor drug resistance is the main reason for the failure of clinical chemotherapy. According to the American Cancer Society, more than 90% of cancer patients die from varying degrees of drug resistance. In the early stage of treatment, tumor cells are sensitive to chemotherapy drugs such as doxorubicin. The drugs accumulate in large quantities in tumor cells and enter the cell nucleus, acting on DNA and causing cell death. During the chemotherapy process, tumor cells mutate, undergo structural and functional evolution, and become insensitive to drugs, which is called tumor drug resistance, and ultimately leads to chemotherapy failure.

[0003] Since Beidler first discovered the phenomenon of tumor cross-resistance in 1970, reversing tumor multidrug resistance (MDR) has become one of the hot topics of research. my country has rich resources of traditional Chinese medicine and a wide range of pharmacological effects. Screening MDR reversal agents from traditional Chinese medicine has great potential.

[0004] Whether it is a herbal medicine or a chemical medicine, there are few methods for evaluating its efficacy. CN110325851A discloses a method for evaluating the efficacy of a drug that reverses tumor multidrug resistance. It uses experimental animals for in vivo testing, requires experimental animals, and has different system errors under control, so the test cost is low. CN102746986A discloses a method for simulating cell spheroid invasion and drug evaluation in a hypoxic microenvironment of a tumor. A polydimethylsiloxane micropit chip that can be used for cell inoculation-sedimentation-aggregation into spheres is used to obtain cell spheres with a specific patterned arrangement. After embedding in a collagen extracellular matrix, a patterned arrangement of the cell spheres in a three-dimensional matrix is ​​achieved for subsequent real-time observation of invasion and related drug evaluation research. Summary of the invention

[0005] In view of the above problems, the present invention proposes an in vitro method for evaluating the efficacy of tumor reversal drugs, which uses a polydimethylsiloxane membrane to simulate the control of tumor hypoxia microenvironment and effectively evaluate the efficacy of tumor reversal drugs.

[0006] The invention discloses a method for evaluating the efficacy of a tumor reversal drug, comprising the step of simulating the hypoxic environment of a tumor by using a polydimethylsiloxane membrane.

[0007] In some embodiments of the present invention, preparing the polydimethylsiloxane film comprises the following steps:

[0008] S01, hydrogen silicone oil and polyethylene glycol methyl ether methacrylate are reacted with a catalyst;

[0009] S02, adding vinyltriethoxysilane to react;

[0010] S03, adding hydroxy-terminated polydimethylsiloxane and a catalyst for reaction;

[0011] S04, doped nanoparticles;

[0012] S05, coating, degassing, drying, alkali washing to remove acidic ions, washing with distilled water until neutral, and drying to obtain the polydimethylsiloxane film.

[0013] In some embodiments of the present invention, the catalyst in S01 is chloroplatinic acid.

[0014] In some embodiments of the present invention, the catalyst in S02 is dibutyltin dilaurate.

[0015] In some embodiments of the present invention, the weight ratio of the hydrogen silicone oil, polyethylene glycol methyl ether methacrylate, S01 catalyst, vinyl triethoxysilane, dibutyl tin dilaurate and S03 catalyst is (1-4): (1-2): (0.5-1): (1-3): (3-5): 0.5, preferably 2:1:0.5:1:3:0.5.

[0016] In some embodiments of the present invention, the particle size of the nanoparticles in S04 is 200-250 nm, and the nanoparticles are preferably silicon dioxide particles.

[0017] In some embodiments of the present invention, S04 is doped with 5-15% by weight of the nanoparticles, preferably 5-8%.

[0018] In some embodiments of the present invention, the thickness of the polydimethylsiloxane film is 150-300 μm, preferably 200-250 μm.

[0019] In some embodiments of the present invention, in S01, the reaction is carried out at 70-80° C. for 2.5-3 h.

[0020] In some embodiments of the present invention, in S02, the reaction is carried out at 75-85° C. for 4.5-5.5 hours.

[0021] In some embodiments of the present invention, in S03, the reaction is carried out at 60-70° C. for 2.5-3.5 hours.

[0022] In some embodiments of the present invention, the method further comprises culturing tumor cells with the polydimethylsiloxane membrane and measuring metabolic markers.

[0023] In some embodiments of the present invention, a cyclone drying device is required for drying in S05, and the cyclone drying device includes a heating device, a drying box and a cyclone generator, wherein the heating device is arranged at the inner bottom of the drying box, the cyclone generator is arranged on the heating device and located in the drying box, and the drying container is placed on the cyclone generator;

[0024] The cyclone generator comprises a shell, a bottom ring plate, a diverter ring plate, and a top ring plate; the shell is ring-shaped, an air inlet is arranged on the shell, and an air guide plate is arranged inside the air inlet;

[0025] The bottom surface of the shell is connected to the bottom ring plate, the top surface of the shell is connected to the top ring plate, the flow splitter ring plate is arranged between the bottom ring plate and the top ring plate, the bottom surface of the flow splitter ring plate and the top surface of the bottom ring plate form a bottom air passage, the top surface of the flow splitter ring plate and the bottom surface of the top ring plate form a top air passage, and the outer wall of the flow splitter ring plate and the inner wall of the shell form an annular air passage;

[0026] The inner wall of the bottom ring plate is provided with a small support ring, the bottom of the small support ring is provided with a support platform, and the diameter of the small support ring is larger than the heating diameter of the heating device;

[0027] The inner wall of the diverter ring plate is provided with a medium-sized support ring, a support platform is provided on the medium-sized support ring, and the diameter of the medium-sized support ring is greater than the diameter of the small-sized support ring;

[0028] The diverter ring plate is also provided with a lower drainage ring and an upper drainage ring, the lower drainage ring is provided at the bottom of the medium-sized support ring, the outer wall of the lower drainage ring and the inner wall of the small-sized support ring form a lower drainage channel, and the bottom airway and the lower drainage channel are connected to form a small ascending channel;

[0029] A medium-sized ascending channel is formed between the bottom surface of the upper guide ring and the top surface of the diverter ring plate. The top of the upper guide ring is a trumpet-shaped shape with a larger top and a smaller bottom. The top of the upper guide ring is a large-sized support ring, and the diameter of the large-sized support ring is larger than the diameter of the medium-sized support ring.

[0030] The splitter ring plate is provided with a plurality of air holes, and the top air passage is connected with the small ascending passage and the medium ascending passage respectively through the air holes;

[0031] The top surface of the flow dividing ring plate is provided with a circular groove, the bottom surface of the top ring plate is provided with a circular baffle, the circular baffle is located in the circular groove extending from the circular baffle, the circular groove and the circular baffle form a U-shaped airway, and the annular airway is connected with the top airway through the U-shaped airway;

[0032] The inner wall of the top ring plate is provided with a support ring, a large ascending channel is formed between the inner wall of the support ring and the outer wall of the upper drainage ring, the large ascending channel is connected with the top airway, the outer side wall of the support ring is provided with an extra-large support ring, and the side wall of the extra-large support ring is provided with a plurality of air holes.

[0033] Beneficial effects of the present invention:

[0034] The in vitro method for evaluating the efficacy of tumor reversal drugs of the present invention realizes the control of simulated tumor hypoxia microenvironment through a polydimethylsiloxane membrane, and effectively evaluates the efficacy of tumor reversal drugs.

[0035] The preparation method of the polydimethylsiloxane membrane of the present invention is simple to operate, does not involve complicated instruments, can achieve effective control of different hypoxia levels, and provides a good platform for different tumor growth microenvironments.

[0036] The in vitro tumor reversal drug efficacy evaluation method of the present invention simulates the hypoxic microenvironment in vivo in vitro, is close to the natural growth state of the tumor in vivo, and can accurately evaluate the efficacy of the drug. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 This is a diagram of the preparation process of polydimethylsiloxane membrane.

[0038] Figure 2 A cross-sectional view of a cyclone generator.

[0039] Figure 3 for Figure 2 Partial schematic diagram at point A in the middle.

[0040] Figure 4 for Figure 2 A partial schematic diagram of point B in the middle.

[0041] Figure 5 Schematic diagram of the structure at the bottom of the cyclone generator.

[0042] Figure 6 Schematic diagram of the inside of the cyclone generator casing.

[0043] In the figure: 1 outer shell, 2 air inlet, 3 air guide plate, 4 bottom ring plate, 41 small support ring, 5 splitter ring plate, 51 medium support ring, 52 lower drainage ring, 53 upper drainage ring, 54 large support ring, 6 top ring plate, 61 support ring, 7 extra-large support ring, 100 top air duct, 200 small rising channel, 300 medium rising channel, 400 U-shaped air duct, 500 large rising channel, 600 annular air duct. DETAILED DESCRIPTION

[0044] The following describes the embodiments of the present invention through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention.

[0045] Unless otherwise specified, the examples and comparative examples are parallel tests with the same components, component contents, preparation steps and preparation parameters.

[0046] The mass fraction of active hydrogen in the hydrogen silicone oil is 0.3%, the number average molecular weight of the polyethylene glycol methyl ether methacrylate is 400, and the annual average molecular weight of the hydroxy-terminated polydimethylsiloxane is 35,000.

[0047] Example 1

[0048] A method for evaluating the efficacy of a tumor reversal drug comprises the steps of preparing a polydimethylsiloxane film:

[0049] Take 2 parts by weight of hydrogen silicone oil and 1 part by weight of polyethylene glycol methyl ether methacrylate, add 0.5 parts by weight of chloroplatinic acid, react at 70°C for 3 hours, then add 1 part by weight of vinyl triethoxysilane, react at 75°C for 5 hours, add 3 parts by weight of terminal hydroxyl polydimethylsiloxane, then add 0.5 parts by weight of dibutyltin dilaurate, react at 60°C for 3 hours; dope 200-250nm silica nanoparticles at a weight ratio of 5%, stir evenly, coat on the template, degas, dry, alkaline wash to remove acidic ions, wash with distilled water until neutral, and dry to obtain a polydimethylsiloxane film (~200μm).

[0050] Example 2

[0051] A method for evaluating the efficacy of a tumor reversal drug comprises the steps of preparing a polydimethylsiloxane film:

[0052] Take 2 parts by weight of hydrogen silicone oil and 1 part by weight of polyethylene glycol methyl ether methacrylate, add 0.5 parts by weight of chloroplatinic acid, react at 80°C for 3 hours, then add 1 part by weight of vinyl triethoxysilane, react at 85°C for 5 hours, add 3 parts by weight of terminal hydroxyl polydimethylsiloxane, then add 0.5 parts by weight of dibutyltin dilaurate, react at 70°C for 3 hours; dope 200-250nm silica nanoparticles at a weight ratio of 5%, stir evenly, coat on the template, degas, dry, alkaline wash to remove acidic ions, wash with distilled water until neutral, and dry to obtain a polydimethylsiloxane film (~250μm).

[0053] Example 3

[0054] A method for evaluating the efficacy of a tumor reversal drug comprises the steps of preparing a polydimethylsiloxane film:

[0055] Take 2 parts by weight of hydrogen silicone oil and 1 part by weight of polyethylene glycol methyl ether methacrylate, add 0.5 parts by weight of chloroplatinic acid, react at 70°C for 3 hours, then add 1 part by weight of vinyl triethoxysilane, react at 75°C for 5 hours, add 3 parts by weight of terminal hydroxyl polydimethylsiloxane, then add 0.5 parts by weight of dibutyltin dilaurate, react at 60°C for 3 hours; dope 250-300nm silica nanoparticles at a weight ratio of 8%, stir evenly, coat on the template, degas, dry, alkaline wash to remove acidic ions, wash with distilled water until neutral, and dry to obtain a polydimethylsiloxane film (~200μm).

[0056] Example 4

[0057] A method for evaluating the efficacy of a tumor reversal drug comprises the steps of preparing a polydimethylsiloxane film:

[0058] Take 4 parts by weight of hydrogen silicone oil and 1 part by weight of polyethylene glycol methyl ether methacrylate, add 1 part by weight of chloroplatinic acid, react at 70°C for 3 hours, then add 3 parts by weight of vinyl triethoxysilane, react at 75°C for 5 hours, add 5 parts by weight of terminal hydroxyl polydimethylsiloxane, then add 0.5 parts by weight of dibutyltin dilaurate, react at 60°C for 3 hours; dope 200-250nm silica nanoparticles at a weight ratio of 5%, stir evenly, coat on the template, degas, dry, alkaline wash to remove acidic ions, wash with distilled water until neutral, and dry to obtain a polydimethylsiloxane film (~200μm).

[0059] Example 5

[0060] A method for evaluating the efficacy of a tumor reversal drug comprises the steps of preparing a polydimethylsiloxane film:

[0061] Take 2 parts by weight of hydrogen silicone oil and 2 parts by weight of polyethylene glycol methyl ether methacrylate, add 1 part by weight of chloroplatinic acid, react at 70°C for 3 hours, then add 1 part by weight of vinyl triethoxysilane, react at 75°C for 5 hours, add 3 parts by weight of terminal hydroxyl polydimethylsiloxane, then add 0.5 parts by weight of dibutyltin dilaurate, react at 60°C for 3 hours; dope 200-250nm silica nanoparticles at a weight ratio of 15%, stir evenly, coat on the template, degas, dry, alkaline wash to remove acidic ions, wash with distilled water until neutral, and dry to obtain a polydimethylsiloxane film (~200μm).

[0062] Comparative Example 1

[0063] A method for evaluating the efficacy of a tumor reversal drug comprises the steps of preparing a polydimethylsiloxane film:

[0064] Take 2 parts by weight of hydrogen silicone oil and 1 part by weight of polyethylene glycol methyl ether methacrylate, add 0.5 parts by weight of chloroplatinic acid, react at 70°C for 3 hours, then add 1 part by weight of vinyl triethoxysilane, react at 75°C for 5 hours, add 3 parts by weight of terminal hydroxyl polydimethylsiloxane, then add 0.5 parts by weight of dibutyltin dilaurate, react at 60°C for 3 hours, apply on the template, degas, dry, alkaline wash to remove acidic ions, wash with distilled water until neutral, and dry to obtain a polydimethylsiloxane film (~200μm).

[0065] Use of polydimethylsiloxane membrane:

[0066] Referring to the method of CN102746986A, under the same conditions, fluorescent protein transfected brain glioma cells were inoculated on the polydimethylsiloxane membranes of the embodiment and the comparative example (sterilized by ultraviolet irradiation before use, and certain antibiotics can be added during culture as appropriate). The blank control group was used to detect the expression level of hypoxia-related genes, and the experimental group was used to evaluate the efficacy.

[0067] The results showed that hypoxia-related genes were expressed in Examples 1-5 of the present application, the bands of Examples 1-3 were significantly wider than those of Examples 4 and 5, and Comparative Example 1 had no obvious bands.

[0068] Example 6

[0069] A method for evaluating the efficacy of a tumor reversal drug, wherein S05 requires the use of a cyclone drying device for drying. Figure 2-6 shown.

[0070] The cyclone drying device comprises a heating device, a drying box and a cyclone generator, wherein the heating device is arranged at the inner bottom of the drying box, the cyclone generator is arranged on the heating device and located in the drying box, and the drying container is placed on the cyclone generator.

[0071] The cyclone generator comprises a housing 1, a bottom ring plate 4, a diverter ring plate 5, and a top ring plate 6; the housing 1 is ring-shaped, an air inlet 2 is provided on the housing 1, and an air guide plate 3 is provided inside the air inlet 2;

[0072] The bottom surface of the shell 1 is connected to the bottom ring plate 4, the top surface of the shell 1 is connected to the top ring plate 6, the flow splitter ring plate 5 is arranged between the bottom ring plate 4 and the top ring plate 6, the bottom surface of the flow splitter ring plate 5 and the top surface of the bottom ring plate 4 form a bottom layer of air passage, the top surface of the flow splitter ring plate 5 and the bottom surface of the top ring plate 6 form a top layer of air passage 100, and the outer wall of the flow splitter ring plate 5 and the inner wall of the shell 1 form an annular air passage 600;

[0073] The inner wall of the bottom ring plate 4 is provided with a small support ring 41, the bottom of the small support ring 41 is provided with a support platform, and the diameter of the small support ring 41 is larger than the heating diameter of the heating device;

[0074] The inner wall of the diverter ring plate 5 is provided with a medium-sized support ring 51, and a support platform is provided on the medium-sized support ring 51. The diameter of the medium-sized support ring 51 is greater than the diameter of the small-sized support ring 41.

[0075] The flow dividing ring plate 5 is also provided with a lower guide ring 52 and an upper guide ring 53. The lower guide ring 52 is provided at the bottom of the medium-sized support ring 51. The outer wall of the lower guide ring 52 and the inner wall of the small-sized support ring 41 form a lower guide channel. The bottom air channel and the lower guide channel are connected to form a small ascending channel 200.

[0076] A medium-sized ascending channel 300 is formed between the bottom surface of the upper guide ring 53 and the top surface of the diverter ring plate 5. The top of the upper guide ring 53 is in a trumpet shape with a larger top and a smaller bottom. The top of the upper guide ring 53 is a large support ring 54, and the diameter of the large support ring 54 is greater than the diameter of the medium support ring 51.

[0077] The flow dividing ring plate 5 is provided with a plurality of air holes, and the top air passage 100 is respectively connected with the small ascending passage 200 and the medium ascending passage 300 through the air holes;

[0078] The top surface of the flow dividing ring plate 5 is provided with a circular groove, and the bottom surface of the top ring plate 6 is provided with a circular baffle, and the circular baffle is located in the circular groove extending from the circular baffle, and the circular groove and the circular baffle constitute a U-shaped airway 400, and the annular airway 600 is connected with the top airway 100 through the U-shaped airway 400;

[0079] The inner wall of the top ring plate 6 is provided with a support ring 61, and a large ascending channel 500 is formed between the inner wall of the support ring 61 and the outer wall of the upper guide ring 53. The large ascending channel 500 is connected to the top airway 100, and the outer side wall of the support ring 61 is provided with an extra-large support ring 7, and the side wall of the extra-large support ring 7 is provided with a plurality of air holes.

[0080] Working principle and beneficial effects: Depending on the drying quality, the heating equipment used is different. When the drying quality is small, electric heating equipment can be used. When the drying quality is large and fast drying is required, the heating equipment can be a combustion heating equipment.

[0081] When the drying mass is small, the drying vessel can be placed in the large, medium and small supporting rings as needed, and the heating device heats from the bottom. At the same time, the air inlet 2 supplies air to the inside of the shell 1. The airflow is guided by the air guide plate 3 and blown into the shell 1 along the tangential direction, and rotates and accelerates in the annular airway 600. A part of the airflow enters the top airway 100 from the U-shaped airway 400, and then sprays out from the large, medium and small ascending channels respectively. It is worth noting that the support platform on the small supporting ring 41 and the medium supporting ring 51 is used to support the drying vessel, and is also used to guide the wind direction of the medium and small ascending channels so that the wind direction can flow upward, thereby avoiding the airflow blowing directly on the surface of the drying vessel, causing the dried nanoparticles to be blown up. When the drying vessel is placed on the medium supporting ring 51 or the large supporting ring 54, the small ascending channel 200 located below the drying vessel will not blow the drying vessel over due to the small air volume.

[0082] When the drying mass is large, a large high-temperature resistant drying vessel can be selected and placed on the extra-large support ring 7. At this time, the heating equipment is far away from the drying vessel. At this time, the airflows in the large, medium and small ascending channels will carry the heat of the heating equipment to act on the bottom of the drying vessel and be discharged from the air holes on the side wall of the extra-large support ring 7. The discharged hot air can quickly heat up the drying box and increase the temperature inside the drying box. It is worth noting that if necessary, combustible gas can be used for air delivery, and the heating equipment can ignite the combustion gas to achieve combustion and heating, thereby further accelerating the heating rate.

[0083] With the design of the above structure, no matter whether the drying method is combustion or electric heating, the airflow will not directly act on the surface of the drying dish. The method of blowing air upward from the side of the drying dish can form a negative pressure on the surface of the drying dish, generating air flow, thereby achieving air flow on the surface of the drying dish while preventing the dried particles from being blown away.

[0084] The air inlet 2 is provided with a detection unit to detect the air entering the housing 1. The detection unit detects the gas flow rate based on the change of kinetic energy and internal energy in the finite body (i.e., the space of the annular airway 600) that changes with time. When performing the detection, it is necessary to calculate using the following formula:

[0085]

[0086] Wherein, Q is the flow rate of the gas; E is the sum of the internal energy and kinetic energy of the gas in the annular gas channel 600; ρ is the density of the gas; h is the specific enthalpy; p is the pressure provided by the gas; where E can be obtained by the convection-diffusion formula evolved from the energy conservation equation, which is:

[0087]

[0088] Where J is the diffusion flux of the gas; k is the thermal conductivity; T is the temperature; is the velocity vector of the gas; is the change of kinetic energy and internal energy in the annular airway 600 with time; is the change of kinetic energy and internal energy caused by the transport of gas; The work done on the pressure gradient created by the gas; To conduct heat; is the mass diffusion of gas due to the change of concentration gradient; is the viscous dissipation (equivalent to the stress work done by the gas); S h As heat source.

[0089] The above formula can be used to calculate the gas flow rate in real time, and the detection unit can be used to calculate the speed of the gas entering the shell 1 in real time. Based on the calculation result, it can be determined whether the air pressure in the annular airway 600 is overloaded. When the air pressure is too high, the flow rate will be weakened. When the air pressure is normal, the gas flow rate can be maintained at a stable value without change, thereby avoiding excessive gas pressure in the shell 1 and causing damage to the equipment.

[0090] The preferred specific implementation modes and embodiments of the present invention are described in detail above, but the present invention is not limited to the above implementation modes and embodiments, and various changes can be made within the knowledge scope of those skilled in the art without departing from the concept of the present invention.

Claims

1. A method for evaluating the efficacy of a tumor reversal drug, characterized in that: The method comprises the steps of simulating the hypoxic environment of a tumor by using a polydimethylsiloxane membrane; The preparation of the polydimethylsiloxane film comprises the following steps: S01, hydrogen silicone oil and polyethylene glycol methyl ether methacrylate are reacted with a catalyst; S02, adding vinyltriethoxysilane to react; S03, adding hydroxy-terminated polydimethylsiloxane and a catalyst for reaction; S04, doped nanoparticles; S05, coating, degassing, drying, alkali washing to remove acidic ions, washing with distilled water until neutral, and drying to obtain the polydimethylsiloxane film; The weight ratio of the hydrogen silicone oil, polyethylene glycol methyl ether methacrylate, the catalyst of S01, vinyl triethoxysilane, dibutyl tin dilaurate and the catalyst of S03 is (1-4): (1-2): (0.5-1): (1-3): (3-5): 0.5; In S04, the nanoparticles are doped at a weight ratio of 5-8%.

2. The method for evaluating the efficacy of a tumor reversal drug according to claim 1, characterized in that: The catalyst in S01 is chloroplatinic acid; And / or, the catalyst in S02 is dibutyltin dilaurate.

3. The method for evaluating the efficacy of a tumor reversal drug according to claim 1 or 2, characterized in that: The weight ratio of the hydrogen silicone oil, polyethylene glycol methyl ether methacrylate, the catalyst of S01, vinyl triethoxysilane, dibutyl tin dilaurate and the catalyst of S03 is 2:1:0.5:1:3:0.

5.

4. The method for evaluating the efficacy of a tumor reversal drug according to claim 1 or 2, characterized in that: The particle size of the nanoparticles in S04 is 200-250 nm.

5. The method for evaluating the efficacy of a tumor reversal drug according to claim 4, characterized in that: The nanoparticles in S04 are silica particles.

6. The method for evaluating the efficacy of a tumor reversal drug according to claim 1 or 2, characterized in that: The polydimethylsiloxane film has a thickness of 150-300 μm.

7. The method for evaluating the efficacy of a tumor reversal drug according to claim 6, characterized in that: The thickness of the polydimethylsiloxane film is 200-250 μm.

8. The method for evaluating the efficacy of a tumor reversal drug according to claim 1 or 2, characterized in that: In S01, the reaction was carried out at 70-80°C for 2.5-3h; and / or, in S02, reacting at 75-85° C. for 4.5-5.5 h; And / or, in S03, react at 60-70°C for 2.5-3.5h.

9. The method for evaluating the efficacy of a tumor reversal drug according to claim 1 or 2, characterized in that: The method also includes the steps of culturing tumor cells with the polydimethylsiloxane membrane and measuring metabolic markers.

10. The method for evaluating the efficacy of a tumor reversal drug according to claim 1 or 2, characterized in that: In S05, a cyclone drying device is used for drying, and the cyclone drying device includes a heating device, a drying box and a cyclone generator, wherein the heating device is arranged at the inner bottom of the drying box, the cyclone generator is arranged on the heating device and is located in the drying box, and the drying container is placed on the cyclone generator; The cyclone generator comprises a housing (1), a bottom ring plate (4), a flow distribution ring plate (5), and a top ring plate (6); the housing (1) is ring-shaped, an air inlet (2) is provided on the housing (1), and an air guide plate (3) is provided inside the air inlet (2); The bottom surface of the outer shell (1) is connected to the bottom ring plate (4), the top surface of the outer shell (1) is connected to the top ring plate (6), the flow dividing ring plate (5) is arranged between the bottom ring plate (4) and the top ring plate (6), a bottom air passage is formed between the bottom surface of the flow dividing ring plate (5) and the top surface of the bottom ring plate (4), a top air passage (100) is formed between the top surface of the flow dividing ring plate (5) and the bottom surface of the top ring plate (6), and an annular air passage (600) is formed between the outer wall of the flow dividing ring plate (5) and the inner wall of the outer shell (1); The inner wall of the bottom ring plate (4) is provided with a small support ring (41), the bottom of the small support ring (41) is provided with a support platform, and the diameter of the small support ring (41) is larger than the heating diameter of the heating device; The inner wall of the flow dividing ring plate (5) is provided with a medium-sized support ring (51), a support platform is provided on the medium-sized support ring (51), and the diameter of the medium-sized support ring (51) is larger than the diameter of the small-sized support ring (41); The flow dividing ring plate (5) is further provided with a lower guide ring (52) and an upper guide ring (53); the lower guide ring (52) is provided at the bottom of the medium-sized support ring (5); the outer wall of the lower guide ring (52) and the inner wall of the small-sized support ring (41) form a lower guide channel; the bottom air channel and the lower guide channel are connected to form a small ascending channel (200); A medium-sized ascending channel (300) is formed between the bottom surface of the upper guide ring (53) and the top surface of the flow dividing ring plate (5); the top of the upper guide ring (53) is in the shape of a trumpet with a larger top and a smaller bottom; the top of the upper guide ring (53) is a large-sized support ring (54); the diameter of the large-sized support ring (54) is greater than the diameter of the medium-sized support ring (51); The flow dividing ring plate (5) is provided with a plurality of air holes, and the top air channel (100) is respectively connected with the small ascending channel (200) and the medium ascending channel (300) through the air holes; The top surface of the flow dividing ring plate (5) is provided with a circular groove, the bottom surface of the top ring plate (6) is provided with a circular baffle, the circular baffle is located in the circular groove extending from the circular baffle, the circular groove and the circular baffle form a U-shaped airway (400), and the annular airway (600) is connected to the top airway (100) through the U-shaped airway (400); The inner wall of the top ring plate (6) is provided with a support ring (61), a large ascending channel (500) is formed between the inner wall of the support ring (61) and the outer wall of the upper guide ring (53), the large ascending channel (500) is communicated with the top airway (100), the outer side wall of the support ring (61) is provided with an extra-large support ring (7), and the side wall of the extra-large support ring (7) is provided with a plurality of air holes.

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