HPV nucleic acid amplification kit and use method

Abstract

The present invention provides a nucleic acid amplification kit for HPV and a method of use, and relates to the technical field of nucleic acid amplification kits. The nucleic acid amplification kit comprises a main box body, a first placement box is slidably connected to the interior of the lower end of one side of the main box body, a mounting cover is fixedly connected to one side of the inner bottom surface of the main box body, a plurality of limit grooves are provided on the inner walls of the front and rear ends of the mounting cover, a plurality of limit blocks are fixedly connected to the outer walls of the front and rear ends of the first placement box, and the limit blocks are slidably connected inside the limit grooves. The present invention can rotate the bidirectional threaded rod by rotating the first rotating head, so that the first threaded sleeve and the first connecting frame drive the second placement box to move toward the outside of the main box body at the same time, so that it is convenient for the staff to take out the disposable sampling gloves and the disposable secretion cotton swab from the inside of the first placement clamping block and the second placement clamping block, respectively, and greatly avoid the occurrence of cross infection.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid amplification kits, and in particular to an HPV nucleic acid amplification kit and a use method thereof. Background Art

[0002] HPV, also known as human papillomavirus, belongs to the genus Papillomavirus A of the family Papovaviridae. It is a spherical DNA virus that can cause proliferation of the squamous epithelium of the human skin and mucous membranes. At the same time, skin-type HPV infection is very common in the population, such as common common warts, toe warts, flat warts, etc., but the specific infection rate cannot be obtained. In the process of human papillomavirus inspection, nucleic acid amplification kits are usually used.

[0003] HPV nucleic acid amplification kits are usually used to detect high-risk human papillomavirus DNA in samples such as genital and urinary tract secretions, scrapings, and biopsy tissues. They are used for auxiliary diagnosis of high-risk human papillomavirus infection and monitoring the efficacy of drug treatment for infected patients.

[0004] However, traditional HPV nucleic acid amplification kits mostly require patients or staff to open the lid when taking the amplification reagent or placing the sample tube. However, during the process of opening the lid multiple times, contaminants can easily affect other test tubes, thereby increasing the occurrence of secondary cross-infection and greatly reducing the effectiveness of the test. Therefore, technical improvements are urgently needed. Summary of the invention

[0005] 1. Technical issues to be resolved

[0006] In view of the deficiencies in the prior art, the present invention provides an HPV nucleic acid amplification kit and a method of use, which solves the problem that traditional nucleic acid amplification kits are inconvenient for staff to place a small number of sample test tubes, and solves the problem that traditional nucleic acid amplification kits mostly require patients or staff to open the box lid when taking amplification reagents or placing sample test tubes, which easily causes secondary cross-infection of multiple test tubes.

[0007] (II) Technical solution

[0008] To achieve the above objectives, the present invention is implemented through the following technical solutions: a HPV nucleic acid amplification kit and a method of use, comprising a main box body, a first placement box is slidably connected to the interior of the lower end of one side of the main box body, a mounting cover is fixedly connected to one side of the inner bottom surface of the main box body, a plurality of limit grooves are provided on the inner walls at the front and rear ends of the mounting cover, a plurality of limit blocks are fixedly connected to the outer walls at the front and rear ends of the first placement box, the limit blocks are slidably connected to the interior of the limit grooves, and a plurality of amplification agent placement grooves are provided inside the first placement box;

[0009] A second adjustment chamber is provided at the center of the lower end of the main box body, a bidirectional threaded rod is rotatably connected to the interior of the second adjustment chamber, the front end and the rear end of the bidirectional threaded rod are sleeved with a first threaded sleeve, the upper part of the first threaded sleeve is fixedly connected to a first connecting frame, one end of the first connecting frame away from the center of the main box body is fixedly connected to a second placement box, and the second placement boxes penetrate the main box body to the outside of the main box body;

[0010] The lower end of the main box body is provided with two third adjustment chambers, two fourth adjustment chambers and a plurality of fifth adjustment chambers in the interior away from the mounting cover, the interior of the third adjustment chamber is rotatably connected with a first rotating rod, the interior of the fourth adjustment chamber is rotatably connected with a second rotating rod, the outer walls of the first rotating rod and the second rotating rod close to one end of the center of the main box body are fixedly connected with two first bevel gears, the interiors of the plurality of fifth adjustment chambers are rotatably connected with a one-way threaded rod, one side of the one-way threaded rod passes through the fifth adjustment chamber and extends to the third adjustment chamber and the fourth adjustment chamber respectively, and is fixedly connected with a second bevel gear, the first bevel gear and the second bevel gear are meshed with each other, the rod body of the one-way threaded rod is sleeved with a second threaded sleeve, the upper surface of the second threaded sleeve is fixedly connected with a second connecting frame, one side of the second connecting frame is fixedly connected with a test tube placement box, the interior of the test tube placement box is provided with a test tube placement hole, one side of the main box body is provided with a plurality of sealing grooves, and the side of the test tube placement box away from the second connecting frame is fixedly connected with a sealing plate;

[0011] A box cover is arranged at the upper end of the main box body, a second handle is fixedly connected to the middle of the upper surface of the box cover, and push lock buckles are fixedly connected to the connection between the upper parts of the front and rear outer walls of the main box body and the box cover.

[0012] Preferably, an adjustment groove is provided on one side of the inner bottom surface of the main box body, a first adjustment cavity is provided on one side of the lower end of the main box body, an adjustment tooth roller is rotatably connected to the inside of the adjustment groove, a plurality of first teeth are fixedly connected to one side of the lower surface of the first placement box, mounting grooves are provided on both sides of the bottom surface of the first adjustment cavity, a spring is fixedly connected to the bottom surface of the mounting groove, a clamping block is fixedly connected to the upper part of the spring, a guide plate is fixedly connected to the middle part of the first adjustment cavity, an adjustment plate is slidably connected to the outer wall of the guide plate, arc-shaped clamping grooves are provided on both sides of the lower surface of the adjustment plate, and a plurality of second teeth are fixedly connected to the upper surface of the adjustment plate.

[0013] Preferably, the first teeth and the second teeth are meshed with the adjusting tooth roller, and the clamping blocks are engaged with the arc-shaped clamping grooves.

[0014] Preferably, a first handle is fixedly connected to the lower portion of the outer wall of one side of the first placement box.

[0015] Preferably, the rear end of the bidirectional threaded rod passes through the second adjustment cavity and is fixedly connected to the outside of the main box body with a first rotating head.

[0016] Preferably, the second placement box is internally fixedly connected with a first placement clamping block and a second placement clamping block respectively.

[0017] Preferably, one end of the first rotating rod and the second rotating rod away from the center of the main box body respectively passes through the third adjustment cavity and the fourth adjustment cavity to the outside of the main box body and is fixedly connected to a second rotating head.

[0018] A method for using a HPV nucleic acid amplification kit comprises the following steps:

[0019] S1. The first placement box can be moved outward by pulling the first handle. At this time, the adjustment tooth roller can be rotated under the action of the first tooth, and then the adjustment plate can slide on the plate body of the guide plate under the action of the second tooth. At this time, the arc-shaped card slot and the card block are mutually engaged, so that the first placement box is more stable and less likely to shake when being pulled out;

[0020] S2. By pulling out the first placement box, it is convenient for the staff to take the amplification reagent solution from the inside of the main box body, avoiding the staff from opening the box cover when taking the amplification reagent solution, thereby reducing the contamination of the test tube sample during actual use and greatly improving the accuracy of the detection;

[0021] S3. The bidirectional threaded rod can be rotated by rotating the first rotating head, so that the first threaded sleeve and the first connecting frame drive the second placement box to move toward the outside of the main box body at the same time, so that it is convenient for the staff to take out the disposable sampling gloves and the disposable secretion cotton swab from the inside of the first placement clamping block and the second placement clamping block respectively, which greatly avoids the occurrence of cross infection;

[0022] S4. When the patient has extracted the secretion sample through the disposable secretion cotton swab, the first rotating rod and the second rotating rod can be rotated respectively by rotating the corresponding second rotating head. At this time, under the action of the first bevel gear and the second bevel gear, the corresponding test tube placement box will move toward the outside of the main box body. At this time, the patient will put the test tubes containing the secretions into the test tube placement hole in sequence, so as to achieve the purpose of placing multiple test tubes separately, further reduce the occurrence of poor infection phenomenon, and greatly improve the accuracy of monitoring. At the same time, by rotating a second rotating head, the purpose of moving the corresponding two test tube placement boxes outward can be achieved, without moving all the test tube placement boxes out to the inside of the main box body, so that when the patient places the test tube, it will not affect other test tubes, which greatly improves the practical performance of the device;

[0023] S5. Finally, by opening the push lock, the staff can open the box cover conveniently, and then it is convenient for the staff to take out multiple test tubes at the same time, bringing convenience to the staff.

[0024] (III) Beneficial effects

[0025] The present invention provides a HPV nucleic acid amplification kit and a method of use. It has the following beneficial effects:

[0026] 1. The present invention provides a nucleic acid amplification kit for HPV and a method of use. The nucleic acid amplification kit can move a first placement box outward by pulling a first handle. At this time, the adjustment tooth roller can be rotated under the action of the first tooth, and then the adjustment plate can slide on the plate body of the guide plate under the action of the second tooth. At this time, the arc-shaped card slot and the card block are mutually engaged, so that the first placement box is more stable and less prone to shaking when pulled out. At the same time, by pulling out the first placement box, it is convenient for staff to take the amplification reagent solution from the inside of the main box body, avoiding the staff from opening the box cover when taking the amplification reagent solution, thereby reducing the contamination of the test tube sample during actual use and greatly improving the accuracy of the detection.

[0027] 2. The present invention provides an HPV nucleic acid amplification kit and a method of use. The nucleic acid amplification kit can rotate the bidirectional threaded rod by rotating the first rotating head, so that the first threaded sleeve and the first connecting frame drive the second placement box to move toward the outside of the main box body at the same time, thereby facilitating the staff to take out the disposable sampling gloves and the disposable secretion cotton swab from the inside of the first placement clamping block and the second placement clamping block respectively, thereby greatly avoiding the occurrence of cross infection.

[0028] 3. The present invention provides an HPV nucleic acid amplification kit and a method of use. The nucleic acid amplification kit can respectively rotate the first rotating rod and the second rotating rod by rotating the corresponding second rotating head. At this time, under the action of the first bevel gear and the second bevel gear, the corresponding test tube placement box will move toward the outside of the main box body. At this time, the patient will then place the test tubes containing secretions into the test tube placement holes in sequence, thereby achieving the purpose of placing multiple test tubes separately, further reducing the occurrence of poor infection, and greatly improving the accuracy of monitoring. At the same time, by rotating a second rotating head, the corresponding two test tube placement boxes can be moved outward without moving all the test tube placement boxes to the inside of the main box body, so that when the patient places the test tube, it will not affect other test tubes, greatly improving the practical performance of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a schematic diagram of the overall structure of the nucleic acid amplification kit proposed by the present invention;

[0030] Figure 2 This is a schematic diagram of the structure of pulling out the first placement box and the second placement box of the nucleic acid amplification kit provided by the present invention;

[0031] Figure 3 This is a schematic diagram of the front cross-section structure of the nucleic acid amplification kit proposed by the present invention;

[0032] Figure 4 This is a schematic diagram of the cross-sectional structure of the nucleic acid amplification kit proposed by the present invention;

[0033] Figure 5 It is a schematic diagram of the front cross-section structure of the first regulating chamber, regulating groove and first placement box of the nucleic acid amplification kit proposed by the present invention;

[0034] Figure 6 For the present invention Figure 4 The enlarged schematic diagram of point A in the middle;

[0035] Figure 7 For the present invention Figure 4 A magnified schematic diagram of point B in the middle.

[0036] Among them, 1. main box body; 2. push lock buckle; 3. first placement box; 4. adjustment slot; 5. adjustment tooth roller; 6. first tooth; 7. first adjustment cavity; 8. installation slot; 9. spring; 10. card block; 11. guide plate; 12. adjustment plate; 13. arc card slot; 14. second tooth; 15. first handle; 16. amplification agent placement slot; 17. second adjustment cavity; 18. bidirectional threaded rod; 19. first threaded sleeve; 20. first connecting frame; 21. second placement box; 22. first placement card block; 23. second placement The first and second adjusting chambers are as follows: 1. a first rotating block; 2. a first rotating head; 2. a third adjusting chamber; 2. a first rotating rod; 2. a fourth adjusting chamber; 2. a second rotating rod; 2. a second rotating head; 3. a first bevel gear; 3. a fifth adjusting chamber; 3. a one-way threaded rod; 3. a second bevel gear; 3. a second threaded sleeve; 3. a second connecting frame; 3. a test tube placement box; 3. a test tube placement hole; 3. a sealing plate; 3. a sealing groove; 40. a mounting cover; 41. a limit groove; 42. a limit block; 43. a box cover; 44. a second handle. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] Example:

[0039] like Figure 1-7 As shown, an embodiment of the present invention provides a nucleic acid amplification kit for HPV and a method of use, comprising a main box body 1, a first placement box 3 is slidably connected to the interior of the lower end of one side of the main box body 1, a mounting cover 40 is fixedly connected to one side of the inner bottom surface of the main box body 1, a plurality of limit grooves 41 are provided on the inner walls of the front and rear ends of the mounting cover 40, a plurality of limit blocks 42 are fixedly connected to the outer walls of the front and rear ends of the first placement box 3, the limit blocks 42 are slidably connected to the interior of the limit grooves 41, and a plurality of amplification agent placement grooves 16 are provided inside the first placement box 3;

[0040] A second adjusting chamber 17 is provided at the center of the lower end of the main box body 1. A bidirectional threaded rod 18 is rotatably connected to the interior of the second adjusting chamber 17. The front and rear ends of the bidirectional threaded rod 18 are sleeved with first threaded sleeves 19. The upper part of the first threaded sleeves 19 is fixedly connected to a first connecting frame 20. One end of the first connecting frame 20 away from the center of the main box body 1 is fixedly connected to a second placement box 21. The second placement box 21 passes through the main box body 1 to the outside of the main box body 1.

[0041] Two third adjustment chambers 25, two fourth adjustment chambers 27 and a plurality of fifth adjustment chambers 31 are respectively provided in the interior of the lower end of the main box body 1 away from the mounting cover 40, the interior of the third adjustment chamber 25 is rotatably connected to the first rotating rod 26, the interior of the fourth adjustment chamber 27 is rotatably connected to the second rotating rod 28, the outer walls of the first rotating rod 26 and the second rotating rod 28 near the center of the main box body 1 are fixedly connected to two first bevel gears 30, the interiors of the plurality of fifth adjustment chambers 31 are rotatably connected to a one-way threaded rod 32, one side of the one-way threaded rod 32 passes through the fifth adjustment chamber 31 and extends respectively A second bevel gear 33 is fixedly connected to the inside of the third regulating chamber 25 and the fourth regulating chamber 27, the first bevel gear 30 and the second bevel gear 33 are meshed with each other, the rod body of the one-way threaded rod 32 is sleeved with a second threaded sleeve 34, the upper surface of the second threaded sleeve 34 is fixedly connected to a second connecting frame 35, one side of the second connecting frame 35 is fixedly connected to a test tube placement box 36, the inside of the test tube placement box 36 is provided with a test tube placement hole 37, one side of the main box body 1 is provided with a plurality of sealing grooves 39, and a sealing plate 38 is fixedly connected to the side of the test tube placement box 36 away from the second connecting frame 35;

[0042] A box cover 43 is provided at the upper end of the main box body 1, a second handle 44 is fixedly connected to the middle of the upper surface of the box cover 43, and push lock buckles 2 are fixedly connected to the connection between the upper parts of the front and rear outer walls of the main box body 1 and the box cover 43.

[0043] An adjusting groove 4 is provided on one side of the bottom surface of the inner part of the main box body 1, a first adjusting cavity 7 is provided on the inner part of one side of the lower end of the main box body 1, an adjusting toothed roller 5 is rotatably connected to the inner part of the adjusting groove 4, a plurality of first teeth 6 are fixedly connected to one side of the lower surface of the first placement box 3, mounting grooves 8 are provided on both sides of the bottom surface of the first adjusting cavity 7, a spring 9 is fixedly connected to the bottom surface of the mounting groove 8, a clamping block 10 is fixedly connected to the upper part of the spring 9, a guide plate 11 is fixedly connected to the middle part of the first adjusting cavity 7, an adjusting plate 12 is slidably connected to the outer wall of the guide plate 11, arc-shaped clamping grooves 13 are provided on both sides of the lower surface of the adjusting plate 12, a plurality of second teeth 14 are fixedly connected to the upper surface of the adjusting plate 12, the first teeth 6 and the second teeth 14 are meshed with the adjusting toothed roller 5, the clamping blocks 10 are clamped and matched with the arc-shaped clamping grooves 13, the lower part of the outer wall of one side of the first placement box 3 is fixedly connected The first handle 15, the rear end of the bidirectional threaded rod 18 passes through the second adjusting chamber 17 to the outside of the main box body 1 and is fixedly connected to the first rotating head 24, the interior of the second placement box 21 is respectively fixedly connected to the first placement clamping block 22 and the second placement clamping block 23, the first rotating rod 26 and the second rotating rod 28 are respectively connected to the third adjusting chamber 25 and the fourth adjusting chamber 27 at one end away from the center of the main box body 1 and are fixedly connected to the outside of the main box body 1 with the second rotating head 29, the bidirectional threaded rod 18 can be rotated by rotating the first rotating head 24, and then the first threaded sleeve 19 and the first connecting frame 20 drive the second placement box 21 to move toward the outside of the main box body 1 at the same time, so as to facilitate the staff to take out the disposable sampling gloves and the disposable secretion cotton swab from the inside of the first placement clamping block 22 and the second placement clamping block 23, respectively, which greatly avoids the occurrence of cross infection.

[0044] A novel multi-stage wind coal separation machine working method comprises the following steps:

[0045] S1. The first placement box 3 can be moved outward by pulling the first handle 15. At this time, the adjustment tooth roller 5 can be rotated under the action of the first tooth 6, and then the adjustment plate 12 can slide on the plate body of the guide plate 11 under the action of the second tooth 14. At this time, the arc-shaped card slot 13 and the card block 10 are mutually engaged, so that the first placement box 3 is more stable and less likely to shake when being pulled out;

[0046] S2. By pulling out the first placement box 3, it is convenient for the staff to take the amplification reagent solution from the inside of the main box body 1, avoiding the staff from opening the box cover 43 when taking the amplification reagent solution, thereby reducing the contamination of the test tube sample during actual use, and greatly improving the accuracy of the detection;

[0047] S3. The bidirectional threaded rod 18 can be rotated by rotating the first rotating head 24, so that the first threaded sleeve 19 and the first connecting frame 20 drive the second placement box 21 to move toward the outside of the main box body 1 at the same time, so that it is convenient for the staff to take out the disposable sampling gloves and the disposable secretion cotton swab from the inside of the first placement clamping block 22 and the second placement clamping block 23, respectively, which greatly avoids the occurrence of cross infection;

[0048] S4. When the patient has extracted the secretion sample with the disposable secretion cotton swab, the first rotating rod 26 and the second rotating rod 28 can be rotated respectively by rotating the corresponding second rotating head 29. At this time, under the action of the first bevel gear 30 and the second bevel gear 33, the corresponding test tube placement box 36 will move toward the outside of the main box body 1. At this time, the patient will put the test tubes containing the secretions into the test tube placement hole 37 in sequence, so as to achieve the purpose of placing multiple test tubes separately, further reducing the occurrence of poor infection phenomenon, and greatly improving the accuracy of monitoring. At the same time, by rotating a second rotating head 29, the purpose of moving the corresponding two test tube placement boxes 36 outward can be achieved, without moving all the test tube placement boxes 36 out to the inside of the main box body 1, so that when the patient places the test tube, it will not affect other test tubes, which greatly improves the practical performance of the device;

[0049] S5. Finally, by opening the push lock 2, the staff can open the box cover 43, which makes it convenient for the staff to take out multiple test tubes at the same time, bringing convenience to the staff.

[0050] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A HPV nucleic acid amplification kit, comprising a main box body (1), characterized in that: A first placement box (3) is slidably connected to the interior of the lower end of one side of the main box body (1); a mounting cover (40) is fixedly connected to one side of the inner bottom surface of the main box body (1); the inner walls of the front and rear ends of the mounting cover (40) are provided with a plurality of limit grooves (41); the outer walls of the front and rear ends of the first placement box (3) are fixedly connected to a plurality of limit blocks (42); the limit blocks (42) are slidably connected to the interior of the limit grooves (41); and the interior of the first placement box (3) is provided with a plurality of amplification agent placement grooves (16); A second adjustment chamber (17) is provided at the center of the lower end of the main box body (1), a bidirectional threaded rod (18) is rotatably connected to the interior of the second adjustment chamber (17), a first threaded sleeve (19) is sleeved on the front and rear ends of the bidirectional threaded rod (18), a first connecting frame (20) is fixedly connected to the upper part of the first threaded sleeve (19), a second placement box (21) is fixedly connected to one end of the first connecting frame (20) away from the center of the main box body (1), and the second placement box (21) passes through the main box body (1) to the outside of the main box body (1); Two third adjustment chambers (25), two fourth adjustment chambers (27) and a plurality of fifth adjustment chambers (31) are respectively provided in the interior of the lower end of the main box body (1) away from the mounting cover (40); the interior of the third adjustment chamber (25) is rotatably connected to a first rotating rod (26); the interior of the fourth adjustment chamber (27) is rotatably connected to a second rotating rod (28); the outer walls of one end of the first rotating rod (26) and the second rotating rod (28) close to the center of the main box body (1) are fixedly connected to two first bevel gears (30); the interiors of the plurality of fifth adjustment chambers (31) are rotatably connected to a one-way threaded rod (32); one side of the one-way threaded rod (32) passes through the fifth adjustment chamber (31) and extends to The third regulating chamber (25) and the fourth regulating chamber (27) are fixedly connected with a second bevel gear (33) inside, the first bevel gear (30) and the second bevel gear (33) are meshed with each other, the shaft of the one-way threaded rod (32) is sleeved with a second threaded sleeve (34), the upper surface of the second threaded sleeve (34) is fixedly connected with a second connecting frame (35), one side of the second connecting frame (35) is fixedly connected with a test tube placement box (36), the inside of the test tube placement box (36) is provided with a test tube placement hole (37), one side of the main box body (1) is provided with a plurality of sealing grooves (39), and the side of the test tube placement box (36) away from the second connecting frame (35) is fixedly connected with a sealing plate (38); A box cover (43) is provided at the upper end of the main box body (1), a second handle (44) is fixedly connected to the middle of the upper surface of the box cover (43), and push lock buckles (2) are fixedly connected to the connection points between the upper parts of the front and rear outer walls of the main box body (1) and the box cover (43); An adjustment groove (4) is provided on one side of the bottom surface of the main box body (1), a first adjustment cavity (7) is provided on one side of the lower end of the main box body (1), an adjustment tooth roller (5) is rotatably connected to the inside of the adjustment groove (4), a plurality of first teeth (6) are fixedly connected to one side of the lower surface of the first placement box (3), mounting grooves (8) are provided on both sides of the bottom surface of the first adjustment cavity (7), a spring (9) is fixedly connected to the bottom surface of the mounting groove (8), a clamping block (10) is fixedly connected to the upper part of the spring (9), a guide plate (11) is fixedly connected to the middle part of the first adjustment cavity (7), an adjustment plate (12) is slidably connected to the outer wall of the guide plate (11), arc-shaped clamping grooves (13) are provided on both sides of the lower surface of the adjustment plate (12), and a plurality of second teeth (14) are fixedly connected to the upper surface of the adjustment plate (12).

2. The HPV nucleic acid amplification kit according to claim 1, characterized in that: The first teeth (6) and the second teeth (14) are meshed with the adjusting tooth roller (5), and the clamping blocks (10) are clamped and matched with the arc-shaped clamping grooves (13).

3. The HPV nucleic acid amplification kit according to claim 1, characterized in that: A first handle (15) is fixedly connected to the lower portion of an outer wall of one side of the first placement box (3).

4. The HPV nucleic acid amplification kit according to claim 1, characterized in that: The rear end of the bidirectional threaded rod (18) passes through the second adjustment cavity (17) and is fixedly connected to the outside of the main box body (1) with a first rotating head (24).

5. The HPV nucleic acid amplification kit according to claim 1, characterized in that: The second placement box (21) is internally fixedly connected with a first placement clamping block (22) and a second placement clamping block (23).

6. The HPV nucleic acid amplification kit according to claim 1, characterized in that: One end of the first rotating rod (26) and the second rotating rod (28) away from the center of the main box body (1) respectively passes through the third adjustment cavity (25) and the fourth adjustment cavity (27) to the outside of the main box body (1) and is fixedly connected to a second rotating head (29).

7. A method for using a HPV nucleic acid amplification kit, using the HPV nucleic acid amplification kit according to claim 1, characterized in that: The following steps are involved: S1. The first placement box (3) is moved outward by pulling the first handle (15). At this time, the adjusting tooth roller (5) is rotated under the action of the first tooth (6), and then the adjusting plate (12) is slid on the plate body of the guide plate (11) under the action of the second tooth (14). At this time, the arc-shaped slot (13) and the block (10) are mutually engaged; S2. By pulling out the first placement box (3), it is convenient for the staff to take the amplification reagent solution from the inside of the main box body (1), thereby avoiding the staff from opening the box cover (43) when taking the amplification reagent solution; S3. The bidirectional threaded rod (18) is then rotated by rotating the first rotating head (24), thereby causing the first threaded sleeve (19) and the first connecting frame (20) to drive the second placement box (21) to move toward the outside of the main box body (1) at the same time, thereby facilitating the staff to take out the disposable sampling gloves and the disposable secretion cotton swab from the inside of the first placement clamping block (22) and the second placement clamping block (23), respectively; S4. When the patient has finished extracting the secretion sample using the disposable secretion cotton swab, the first rotating rod (26) and the second rotating rod (28) are rotated by rotating the corresponding second rotating head (29). At this time, under the action of the first bevel gear (30) and the second bevel gear (33), the corresponding test tube placement box (36) moves toward the outside of the main box body (1). At this time, the patient then sequentially places the test tubes containing the secretions into the test tube placement holes (37). At the same time, by rotating a second rotating head (29), the purpose of moving the corresponding two test tube placement boxes (36) outward is achieved, without having to move all the test tube placement boxes (36) out to the inside of the main box body (1); S5. Finally, the staff can open the box cover (43) by opening the push lock (2).

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