A kind of heat preservation germination substrate and germination method thereof

By using germination matrix made of locust leaves soaked with river sand, humus soil and decayed bacterial solution, the existing germination method has solved the problems of complex management and high energy consumption, and achieved the effect of fast germination speed and low cost.

CN116098035BActive Publication Date: 2025-02-11BIJIE INST OF TRADITIONAL CHINESE MEDICINE
View PDF 3 Cites 0 Cited by

Patent Information

Application Number
CN202310264673.7
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2023-03-17
Publication Date
2025-02-11
Estimated Expiration
2043-03-17

AI Technical Summary

Technical Problem

The existing germination methods are complex in management and energy consumption, making it difficult to effectively ensure the temperature and humidity in the germination matrix, resulting in slow germination speed and high cost.

Method used

The insulation and germination matrix is ​​made of locust leaves soaked in river sand, humus soil and decayed bacterial fluid. It can insulate the heat through fermentation and heating, and secrete enzymes and beneficial bacteria to inhibit the decayed bacteria and improve the quality of seed buds.

Benefits of technology

It achieves the shortening of seed germination time without additional temperature and watering, improves the thriving degree of seed buds, reduces planting costs, and is simple to manage and has low energy consumption.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN116098035B_ABST
    Figure CN116098035B_ABST
Patent Text Reader

Abstract

The present invention provides a heat preservation germination matrix and a germination method thereof, and belongs to the field of plant cultivation technology. The heat preservation germination matrix of the present invention is made of the following raw materials in parts by weight: 4 to 6 parts of river sand, 8 to 12 parts of humus soil, and 1 to 3 parts of locust leaves soaked in a decomposing bacterial solution; the decomposing bacterial solution contains Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei, and yeast. The present invention uses a decomposed matrix for germination, which not only plays a heat preservation role, but also can secrete a variety of enzymes, secondary metabolites and beneficial bacteria, inhibit the growth and reproduction of spoilage bacteria, reduce seed decay during the germination process, improve the quality of seed buds, do not need to apply additional temperature and watering, shorten the germination time, and improve the thriving degree of seed buds.
Need to check novelty before this filing date? Find Prior Art

Description

Technical Field

[0001] The invention relates to the technical field of plant cultivation, in particular to a heat-insulating germination substrate and a germination method thereof. Background Art

[0002] Direct seeding refers to the act of sowing dry seeds directly into the soil without artificial germination and seedling raising, so that the seeds germinate in the soil, thereby reducing the number of transplanting steps. However, the conditions required for seed germination are relatively harsh. Low or high temperature, low or high humidity, will all lead to the situation where the seeds do not germinate. Direct seeding makes it difficult to regulate soil conditions, making it impossible to predict the germination of seeds. In addition, transplanting seeds after germination can screen strong seedlings and remove unqualified seedlings, and the planting density can be controlled during planting, thereby reducing seed waste. Therefore, direct seeding of seedlings is currently the most commonly used method for plant cultivation.

[0003] Germination, namely providing the most ideal germination environment for seeds, allowing seeds to sprout as soon as possible, and making seedlings more robust. At present, germination is usually to place seeds in a germination matrix, and then place the matrix and seeds together indoors, and promote seed germination by artificially controlling the temperature and humidity of the external environment. However, the seeds are directly in contact with the germination matrix, and it is difficult to effectively ensure the temperature and humidity in the germination matrix by regulating the temperature of the external environment, and this method is complex to manage and consumes a lot of energy, which undoubtedly increases the planting cost. Therefore, it is urgent to provide a germination matrix and germination method that are simple to manage, have low energy consumption, are low in cost, and have a fast germination speed. Summary of the invention

[0004] The object of the present invention is to provide a heat preservation germination substrate and a germination method thereof. The present invention uses a composting substrate for germination, which not only plays a heat preservation role, but also can secrete a variety of enzymes, secondary metabolites and beneficial bacteria, inhibit the growth and reproduction of spoilage bacteria, reduce seed decay during the germination process, improve the quality of seed sprouts, do not need to apply additional temperature and water, shorten the germination time, and improve the thriving degree of seed sprouts.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] The present invention provides a heat preservation and germination matrix, which is made of the following raw materials in parts by weight: 4 to 6 parts of river sand, 8 to 12 parts of humus soil, and 1 to 3 parts of locust tree leaves soaked in a decomposed bacterial liquid;

[0007] The decomposed bacterial liquid contains Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei and yeast.

[0008] Preferably, the volume mass ratio of the decomposed bacteria liquid and the locust tree leaves is 10 ml: (1-4) g.

[0009] Preferably, the total effective viable count of Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei, and yeast is ≥ 80.0×10 8 / g; the ratio of the number of live bacteria of Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei and yeast is (1-3): (1-3): (0.5-2): (0.5-2): (1-3); the mass concentration of the decomposed bacterial liquid is 12-15%.

[0010] Preferably, the germination matrix is ​​obtained by mixing the river sand, the humus soil and the Sophora japonica leaves soaked in the decomposing bacteria liquid, and the mixture is drained until there is no flowing water on the surface.

[0011] The present invention also provides a germination method using the germination matrix, comprising the following steps:

[0012] (1) mixing seeds with the germination medium to obtain a seed mixture;

[0013] (2) laying the seed mixture on a germination bed constructed of a heat-insulating material;

[0014] Before laying the seed mixture, a layer of leaves soaked in mature bacteria liquid is laid on the germination bed, and after laying the seed mixture, another layer of leaves soaked in mature bacteria liquid is covered.

[0015] Preferably, the seeds are treated with a mixed solution of 98% fludioxonil and gibberellin before being mixed with the germination substrate;

[0016] In the mixed solution, the concentration of 98% fludioxonil is 1.5-2 μl / L, and the concentration of gibberellin is 120-180 mg / ml; the treatment time is 30-60 min.

[0017] Preferably, the mass ratio of the seeds to the germination medium is 1:(3-6).

[0018] Preferably, the leaves soaked in the fermentation solution are Sophora japonica leaves soaked in the fermentation solution in the above-mentioned germination matrix.

[0019] Preferably, the seed mixture is laid with a thickness of 8 to 12 cm.

[0020] Preferably, the laying thickness of the leaves soaked in the decomposing bacteria liquid is 10 to 15 cm; the covering thickness of the leaves soaked in the decomposing bacteria liquid is 10 to 15 cm.

[0021] The present invention provides a heat preservation germination matrix and a germination method thereof. The present invention uses river sand with ventilation function, humus soil with moisture retention function and locust tree leaves treated with a decomposing bacteria liquid as the matrix, and mixes the seeds so that the substrate ferments and generates heat during the seed germination process, which not only plays a heat preservation role, but also can secrete a variety of enzymes, secondary metabolites and beneficial bacteria, inhibit the growth and reproduction of spoilage bacteria, reduce the decay of seeds during the germination process, and improve the quality of seed buds. And by laying and covering locust tree leaves treated with a decomposing bacteria liquid on the top and bottom of the seeds, the temperature for seed germination is further guaranteed. This method does not require additional application of temperature and watering, can meet the germination requirements outdoors, reduces costs, shortens the germination time, and can improve the degree of bud growth, with great economic benefits. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The germination state of the seeds of Polygonatum cyrtonema treated with the germination accelerating medium of Example 1 after germination for one week.

[0023] Figure 2 This is the germination status of the Polygonatum cyrtonema seeds sown outdoors in Experimental Example 2, one week after germination. DETAILED DESCRIPTION

[0024] The invention provides a heat-insulating germination matrix, which is made of the following raw materials in parts by weight: 4-6 parts of river sand, 8-12 parts of humus soil, and 1-3 parts of locust tree leaves soaked in a decomposed bacteria liquid.

[0025] In the present invention, the river sand is preferably sterilized.

[0026] In the present invention, the sterilization treatment is preferably performed by mixing the river sand with carbendazim powder.

[0027] In the present invention, the mass ratio of the river sand to the carbendazim powder is preferably 10kg:100-120mg, and more preferably 10kg:110mg.

[0028] In the present invention, the humus soil is preferably sterilized.

[0029] In the present invention, the sterilization treatment is preferably performed by mixing the humus soil with carbendazim powder.

[0030] In the present invention, the mass ratio of the humus soil to the carbendazim powder is preferably 10kg:100-120mg, and more preferably 10kg:110mg.

[0031] In the present invention, the volume-to-mass ratio of the decomposed fungus liquid to the locust tree leaves is preferably 10 ml:(1-4) g, and more preferably 10 ml:3 g.

[0032] In the present invention, the soaking time is preferably 5 to 10 minutes, more preferably 8 minutes.

[0033] In the present invention, the locust tree leaves are preferably crushed before soaking.

[0034] In the present invention, the particle size of the pulverized locust tree leaves is preferably 0.5 to 0.8 cm, more preferably 0.6 cm.

[0035] In the present invention, the fermented bacterial liquid preferably contains Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei, and yeast.

[0036] In the present invention, the total effective viable count of the Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei, and yeast is preferably greater than or equal to 80.0×10 8 Pieces / g.

[0037] In the present invention, the ratio of the number of live bacteria of Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei and yeast is preferably (1-3): (1-3): (0.5-2): (0.5-2): (1-3), and more preferably 2:2:1:1:2.

[0038] In the present invention, the mass concentration of the decomposed bacteria liquid is preferably 12-15%, and more preferably 14%.

[0039] In the present invention, the germination substrate is preferably prepared by mixing the river sand, the humus soil and the locust tree leaves soaked in the decomposing bacteria solution, and then draining the mixture until there is no flowing water on the surface.

[0040] The present invention also provides a germination method using the germination matrix, comprising the following steps:

[0041] (1) mixing seeds with the germination medium to obtain a seed mixture;

[0042] (2) Spreading the seed mixture on a germination bed constructed with heat-insulating materials.

[0043] The present invention mixes seeds with germination matrix to obtain a seed mixture.

[0044] In the present invention, before the seeds are mixed with the germination substrate, the seeds are preferably treated with a mixed solution of 98% fludioxonil and gibberellin.

[0045] In the present invention, the concentration of 98% fludioxonil in the mixed solution is preferably 1.5 to 2 μl / L, more preferably 2 μl / L.

[0046] In the present invention, in the mixed solution, the concentration of the gibberellin is preferably 120-180 mg / ml, and more preferably 150 mg / ml.

[0047] In the present invention, the treatment is preferably soaking.

[0048] In the present invention, the soaking time is preferably 30 to 60 minutes, more preferably 40 minutes.

[0049] In the present invention, after the seeds are treated with the mixed solution, they are preferably also treated with carbendazim powder.

[0050] In the present invention, the mass ratio of the carbendazim powder to the seeds is preferably 80-120 g:10 kg, and more preferably 100 g:10 kg.

[0051] In the present invention, the mass ratio of the seeds to the germination medium is preferably 1:(3-6), and more preferably 1:4.

[0052] The present invention lays the seed mixture on a germination bed constructed of heat-insulating materials.

[0053] In the present invention, before laying the seed mixture, it is also preferred to lay a layer of leaves soaked in a composting bacteria solution on the germination bed.

[0054] In the present invention, the leaf is preferably a locust tree leaf.

[0055] In the present invention, the volume-to-mass ratio of the decomposed fungus liquid to the locust tree leaves is preferably 10 ml:(1-4) g, and more preferably 10 ml:3 g.

[0056] In the present invention, the locust tree leaves are preferably crushed before soaking.

[0057] In the present invention, the particle size of the pulverized locust tree leaves is preferably 0.5 to 0.8 cm, more preferably 0.6 cm.

[0058] In the present invention, the fermented bacterial liquid preferably contains Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei, and yeast.

[0059] In the present invention, the total effective viable count of the Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei, and yeast is preferably greater than or equal to 80.0×10 8 Pieces / g.

[0060] In the present invention, the ratio of the number of live bacteria of Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei and yeast is preferably (1-3): (1-3): (0.5-2): (0.5-2): (1-3), and more preferably 2:2:1:1:2.

[0061] In the present invention, the mass concentration of the decomposed bacteria liquid is preferably 12-15%, and more preferably 14%.

[0062] In the present invention, the laying thickness of the leaves soaked in the decomposing bacteria liquid is preferably 10 to 15 cm, and more preferably 12 cm.

[0063] In the present invention, after laying the leaves soaked in the fermentation bacteria liquid and before laying the seed mixture, a layer of plastic net is preferably laid.

[0064] In the present invention, the pore size of the plastic mesh is preferably 80-120 meshes, and more preferably 100 meshes.

[0065] In the present invention, after laying the seed mixture, it is preferably covered with a layer of leaves soaked in a composting bacteria solution.

[0066] In the present invention, the leaf is preferably a locust tree leaf.

[0067] In the present invention, the volume-to-mass ratio of the decomposed fungus liquid to the locust tree leaves is preferably 10 ml:(1-4) g, and more preferably 10 ml:3 g.

[0068] In the present invention, the locust tree leaves are preferably crushed before soaking.

[0069] In the present invention, the particle size of the pulverized locust tree leaves is preferably 0.5 to 0.8 cm, more preferably 0.6 cm.

[0070] In the present invention, the fermented bacterial liquid preferably contains Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei, and yeast.

[0071] In the present invention, the total effective viable count of the Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei, and yeast is preferably greater than or equal to 80.0×10 8 Pieces / g.

[0072] In the present invention, the ratio of the number of live bacteria of Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei and yeast is preferably (1-3): (1-3): (0.5-2): (0.5-2): (1-3), and more preferably 2:2:1:1:2.

[0073] In the present invention, the mass concentration of the decomposed bacteria liquid is preferably 12-15%, and more preferably 14%.

[0074] In the present invention, the thickness of the leaves soaked with the decomposing bacteria liquid is preferably 10 to 15 cm, more preferably 12 cm.

[0075] In the present invention, after the seed mixture is laid, and before the leaves soaked with the composting bacteria liquid are covered, a layer of plastic net is preferably also covered.

[0076] In the present invention, the pore size of the plastic mesh is preferably 80-120 meshes, and more preferably 100 meshes.

[0077] In the present invention, the laying thickness of the seed mixture is preferably 8 to 12 cm, more preferably 10 cm.

[0078] In the present invention, the thermal insulation material is preferably one or more of foam, polystyrene board, fiber blanket, aluminum silicate felt, glass wool, rock wool, and expanded perlite, and is more preferably fiber blanket.

[0079] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0080] Example 1

[0081] This embodiment provides a heat preservation and germination matrix, and the specific preparation process is as follows:

[0082] Bacillus licheniformis, Acetobacter acetobacter, Aspergillus oryzae, Trichoderma reesei, and yeast were mixed in a ratio of 2:2:1:1:2 to prepare a culture medium with a viable count of 80.0 × 10 8 The compound bacterial agent is dissolved in water to prepare a 14% concentration of decomposing bacterial liquid. The leaves of Sophora japonica are crushed to a particle size of 0.6 cm, and the leaves of Sophora japonica are soaked in each group of decomposing bacterial liquid for 8 minutes at a ratio of 10 ml of decomposing bacterial liquid: 3 g of Sophora japonica leaves, so that the decomposing bacterial liquid is evenly distributed on the surface of the leaves, which helps to ferment and generate heat during the seed germination process, plays a role in heat preservation, and can also secrete a variety of enzymes, secondary metabolites and beneficial bacteria, inhibit the growth and reproduction of spoilage bacteria, reduce seed rot during the germination process and improve the quality of seed buds.

[0083] The river sand (purchased from Hebei Zexu Building Materials Technology Development Co., Ltd.) was sterilized at a ratio of 10 kg river sand to 110 mg carbendazim powder; the humus soil (purchased from Hongmei Agricultural Facilities Equipment Co., Ltd., Shouguang City, Shandong Province) was sterilized at a ratio of 10 kg humus soil to 110 mg carbendazim powder. 2 kg of soaked locust leaves, 5 kg river sand, and 10 kg humus soil were fully mixed and drained until there was no flowing water on the surface to obtain a germination medium.

[0084] Example 2

[0085] Bacillus licheniformis, Acetobacter aceticus, Aspergillus oryzae, Trichoderma reesei, and yeast were mixed in a ratio of 1:3:0.5:0.5:1 to prepare a culture medium with a viable count of 80.0×108 The compound bacterial agent is dissolved in water to prepare a 12% concentration of decomposing bacterial liquid. The leaves of Sophora japonica are crushed to a particle size of 0.5 cm, and the leaves of Sophora japonica are soaked in each group of decomposing bacterial liquid for 5 minutes at a ratio of 10 ml of decomposing bacterial liquid: 1 g of Sophora japonica leaves, so that the decomposing bacterial liquid is evenly distributed on the surface of the leaves, which helps to ferment and generate heat during the seed germination process, plays a role in heat preservation, and can also secrete a variety of enzymes, secondary metabolites and beneficial bacteria, inhibit the growth and reproduction of spoilage bacteria, reduce seed rot during the germination process and improve the quality of seed buds.

[0086] The river sand (purchased from Hebei Zexu Building Materials Technology Development Co., Ltd.) was sterilized according to the ratio of 10kg river sand: 100mg carbendazim powder; the humus soil (purchased from Hongmei Agricultural Facilities Equipment Co., Ltd., Shouguang City, Shandong Province) was sterilized according to the ratio of 10kg humus soil: 100mg carbendazim powder. 1kg of soaked Sophora japonica leaves, 4kg river sand, and 8kg humus soil were fully mixed and drained until there was no flowing water on the surface to obtain the germination medium.

[0087] Example 3

[0088] Bacillus licheniformis, Acetobacter acetobacter, Aspergillus oryzae, Trichoderma reesei, and yeast were mixed in a ratio of 3:3:2:2:3 to prepare a culture medium with a viable count of 80.0 × 10 8 The compound bacterial agent is dissolved in water to prepare a 12% concentration of decomposing bacterial liquid. The leaves of Sophora japonica are crushed to a particle size of 0.8 cm, and the leaves of Sophora japonica are soaked in each group of decomposing bacterial liquid for 10 minutes at a ratio of 10 ml of decomposing bacterial liquid: 4 g of Sophora japonica leaves, so that the decomposing bacterial liquid is evenly distributed on the surface of the leaves, which helps to ferment and generate heat during the seed germination process, plays a role in heat preservation, and can also secrete a variety of enzymes, secondary metabolites and beneficial bacteria, inhibit the growth and reproduction of spoilage bacteria, reduce seed rot during the germination process and improve the quality of seed buds.

[0089] The river sand (purchased from Hebei Zexu Building Materials Technology Development Co., Ltd.) was sterilized at a ratio of 10 kg river sand to 120 mg carbendazim powder; the humus soil (purchased from Hongmei Agricultural Facilities Equipment Co., Ltd., Shouguang City, Shandong Province) was sterilized at a ratio of 10 kg humus soil to 120 mg carbendazim powder. 3 kg of soaked locust leaves, 6 kg river sand, and 12 kg humus soil were fully mixed and drained until there was no flowing water on the surface to obtain a germination medium.

[0090] Comparative Example 1

[0091] Based on Example 1, this comparative example explored the bacterial species in the composite bacterial agent, the concentration of the decomposed bacterial solution, and the types of leaves. Among them, the bacterial agent formula is as shown in Table 1, set as Group 1, 2, and 3; the concentration of the decomposed bacterial solution is adjusted to 20%, set as Group 4; the locust leaves are replaced with pine needles, set as Group 5. Other operations are the same as Example 1.

[0092] Table 1 Compound bacterial agent strain formula

[0093] Group 1 Group 2 Group 3 Bacillus licheniformis - 2 2 Bacillus subtilis 2 - - Acetobacter 2 - 2 Lactic acid bacteria - 2 - Aspergillus oryzae 1 1 - Aspergillus niger - - 1 Trichoderma reesei 1 1 1 Yeast 2 2 2

[0094] Test Example 1

[0095] The performance of the germination substrate prepared by the above Examples 1 to 3 and Groups 1 to 5 in Comparative Example 1 was investigated using the germination time of Polygonatum cyrtonema seeds and the germination rate after one week of germination as indicators. The specific process is as follows:

[0096] (1) In October, harvest plump, yellow-skinned Polygonatum sibiricum fruits, bag them, and seal the bags. After the flesh undergoes anaerobic respiration, heats up, and rots, soak them in water to separate the seeds and the flesh / juice. Ensure that the flesh on the seed surface is completely washed off, and dry the surface moisture.

[0097] (2) Take 98% fludioxonil, mix them in a ratio of 10 μl fludioxonil: 5 kg water, then add gibberellin to make the concentration of gibberellin 150 mg / ml, and obtain a mixed solution. Soak the polygonatum seeds dried in step (1) in the mixed solution for 40 minutes, and then coat the seed epidermis with a layer of carbendazim powder in a ratio of 100 g carbendazim powder: 10 kg seeds. Carbendazim and gibberellin have a dormancy-breaking effect on polygonatum seeds and can shorten the germination period of seeds.

[0098] (3) The seeds treated in step (2) are mixed with the germination substrates prepared above in a mass ratio of 1:4 to obtain a seed mixture.

[0099] (4) Use a fiber blanket as a base, and use the leaves soaked in the fermentation liquid of the corresponding group to spread on the fiber blanket with a thickness of 12 cm, wrap it around with fiber blanket, and then lay a layer of plastic mesh with a pore size of 100 mesh to make a germination bed. Spread the seed mixture evenly on the plastic mesh of the germination bed with a thickness of 10 cm, cover the seeds with a plastic mesh with a pore size of 100 mesh, and then cover it with a 12 cm thick layer of leaves soaked in the fermentation liquid of the corresponding group. After this treatment, the upper and lower layers of leaves of the seed mixture ferment and generate heat, which can ensure the temperature for seed germination. Then wait for germination.

[0100] The germination time of each group and the germination rate after one week of germination were counted, and the results are shown in Table 2. Among them, the germination substrate of Example 1 was used to treat the Polygonatum cyrtonema seeds. The germination state of the seeds after one week of germination was as follows Figure 1 shown.

[0101] Table 2 Germination time and germination rate of each group after one week of germination

[0102] Group Germination time Germination rate Example 1 35 days 96.5% Example 2 37 days 95.7% Example 3 38 days 95.9% Group 1 45 days 78.3% Group 2 49 days 72.1% Group 3 44 days 82.4% Group 4 41 days 45.7% Group 5 50 days 82.5%

[0103] As can be seen from Table 2, the germination matrix of Examples 1 to 3 can significantly shorten the germination time of Polygonatum sibiricum seeds and improve the germination rate. After Group 1 replaced Bacillus licheniformis, Group 2 replaced Acetobacter, and Group 3 replaced Aspergillus oryzae, the germination time of Polygonatum sibiricum seeds was significantly prolonged, and the germination rate was reduced. After Group 4 increased the concentration of the decomposed bacteria solution, the germination time was not significantly different from that of Examples 1 to 3, but its germination rate was significantly reduced. Subsequent testing showed that the temperature of the germination bed reached 42.3°C during the germination process. It is speculated that Group 4 had a lower germination rate due to the high germination temperature. After Group 5 replaced Sophora japonica leaves, the germination time was prolonged and the germination rate was reduced compared with Examples 1 to 3. It shows that the germination matrix of Examples 1 to 3 of the present invention can significantly shorten the germination time of Polygonatum sibiricum seeds and improve the germination rate.

[0104] Test Example 2

[0105] This test example explored the germination method of Polygonatum cyrtonema seeds. Polygonatum cyrtonema seeds treated according to steps (1) to (3) of Test Example 1 were sown outdoors, forming Group 7; Polygonatum cyrtonema seeds treated according to steps (1) to (2) of Test Example 1 were sown outdoors, forming Group 8. The germination time and germination rate of each group after one week of germination were counted, and the results are shown in Table 3. Among them, the germination status of seeds in Group 8 after one week of germination is as follows Figure 2 shown.

[0106] Table 3 Germination time and germination rate of each group after one week of germination

[0107] Group Germination time Germination rate Example 1 35 days 96.5% Group 7 52 days 87.7% Group 8 60 days 52.4%

[0108] As can be seen from Table 3, by using the germination matrix and germination method of the present invention, the germination time is 35 days and the germination rate is 96.5%; by only using the germination matrix of the present invention and direct sowing Polygonatum cyrtonema seeds outdoors, the germination time is 52 days and the germination rate is 87.7%; while by directly direct sowing Polygonatum cyrtonema seeds outdoors, the germination time reaches 60 days and the germination rate is only 52.4%. This shows that compared with direct sowing Polygonatum cyrtonema seeds outdoors, the germination matrix of the present invention combined with the germination method of the present invention can greatly shorten the germination time and improve the germination rate. Using only the germination matrix of the present invention can also significantly shorten the germination time and improve the germination rate. In addition, from Figure 1 and Figure 2 It can be seen that the germination matrix of the present invention combined with the germination method of the present invention can significantly enhance the vigor of seed buds than that of seed buds directly sown outdoors.

[0109] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A germination method using a heat-insulating germination matrix, characterized in that: The germination matrix is ​​made of the following raw materials in parts by weight: 4-6 parts of river sand, 8-12 parts of humus soil, and 1-3 parts of Sophora japonica leaves soaked in a decomposed bacteria solution; The decomposed bacterial liquid contains Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei, and yeast; The soaking time of the decomposed bacteria liquid is 5 to 10 minutes; The germination accelerating method using the heat-insulating germination accelerating matrix comprises the following steps: (1) mixing seeds with the germination medium to obtain a seed mixture; (2) laying the seed mixture on a germination bed constructed of a heat-insulating material; Before laying the seed mixture, a layer of leaves soaked in a mature bacteria solution is laid on the germination bed, and after laying the seed mixture, another layer of leaves soaked in a mature bacteria solution is covered; The volume mass ratio of the decomposed bacterial liquid to the locust tree leaves is 10 ml: (1-4) g; The total effective viable count of Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei and yeast is ≥80.0×10 8 / g; the ratio of the number of live bacteria of Bacillus licheniformis, Acetobacter, Aspergillus oryzae, Trichoderma reesei and yeast is (1-3): (1-3): (0.5-2): (0.5-2): (1-3); the mass concentration of the decomposed bacterial liquid is 12-15%; Before the seeds are mixed with the germination medium, they are treated with a mixed solution of 98% fludioxonil and gibberellin; In the mixed solution, the concentration of 98% fludioxonil is 1.5-2 μl / L, and the concentration of gibberellin is 120-180 mg / ml; the treatment time is 30-60 min; The mass ratio of the seeds to the germination matrix is ​​1:(3-6).

2. The germination method according to claim 1, characterized in that The germination matrix is ​​obtained by mixing the river sand, the humus soil and the locust tree leaves soaked in the mature bacteria liquid, and the mixture is drained until there is no flowing water on the surface.

3. The germination method according to claim 2, characterized in that The leaves soaked in the fermentation liquid are the leaves of Sophora japonica leaves soaked in the fermentation liquid described in the germination matrix used in claim 1.

4. The germination method according to claim 3, characterized in that The seed mixture is laid with a thickness of 8 to 12 cm.

5. The germination method according to claim 4, characterized in that: The laying thickness of the leaves soaked in the decomposing bacteria liquid is 10-15 cm; the covering thickness of the leaves soaked in the decomposing bacteria liquid is 10-15 cm.

Citation Information

Patent Citations

  • Method for planting ginseng under forest

    CN101496483A

  • Method for decomposing loquat branches by microorganisms

    CN111646864A

  • Zelkova schneideriana container seedling substrate and preparation method thereof

    CN112568091A