Soilless cultivation agricultural planting cabinet and use method thereof
By setting up a real-time microbial detection mechanism and an automatic pore membrane replacement mechanism in the soilless cultivation agricultural planting cabinet, the microbial reproduction problem caused by the nutrient solution is solved, real-time monitoring and early warning of microorganisms in the nutrient solution is achieved, and microorganisms are prevented from cerebralizing plants. Root corrosion improves plant health and yield.
Patent Information
- Application Number
- CN202211095585.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-09-06
- Publication Date
- 2025-02-07
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In soilless cultivation, the reproduction of microorganisms due to the standing of nutrient solution, causing foul odor in water and plant health problems. The circulating supply system accelerates the transmission of microorganisms and endangers the health of plant roots.
A soilless cultivation agricultural planting cabinet is designed, equipped with a real-time microbial detection mechanism and an automatic pore membrane replacement mechanism. By automatically detecting the reproduction of microorganisms in the nutrient solution, and early warning and replacement of the nutrient solution when exceeding the standard to prevent microorganisms from corroding the plant roots.
Real-time detection and early warning of the reproduction of microorganisms in nutrient solution is achieved, which avoids the corrosion of microorganisms on plant roots through the circulation system, improves plant health and yield, and reduces the cost of labor detection and labor intensity.
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Figure CN116138150B_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of soilless cultivation, and more particularly to a soilless cultivation agricultural planting cabinet and a use method thereof. Background Art
[0002] Soilless cultivation, also known as nutrient solution cultivation or hydroponic cultivation, is a cultivation technology that uses nutrient solution containing essential elements for plant growth and development to provide nutrition without natural soil, so that plants can complete their entire life cycle normally. It greatly expands the space for agricultural production, allowing crops to be produced without relying on soil, and has a very broad development prospect.
[0003] Since the water and nutrient solution in the cultivation pot are in a static state for a long time, it is easy for microorganisms in the air to enter the water and multiply, which can easily cause water odor and further cause pollution to the surrounding air environment. Similarly, the reproduction of microorganisms in the water and nutrient solution will also absorb the nutrients in the nutrient solution, causing poor plant growth and affecting plant health and yield.
[0004] In response to this technical problem, Chinese patent application number: 202120482373.2 provides an agricultural soilless cultivation device, which uses a liquid pump and a series of liquid transmission mechanisms to deliver nutrient solution from a water tank to a breeding tank to water the plants, and then uses a wastewater collection tank to return the unabsorbed nutrient solution to the water tank for circulation. In this way, a circulating supply of nutrient solution is achieved, thereby solving the problem of microbial reproduction caused by standing still.
[0005] However, in actual use, since it is difficult to thoroughly disinfect and sterilize the equipment used to prepare the nutrient solution, and the nutrient solution is exposed to the air, a certain amount of bacteria and microorganisms will exist in the nutrient solution. Once these bacteria and microorganisms encounter a suitable breeding environment, such as hot and humid weather, they will multiply a large number of microorganisms and bacteria. Moreover, due to the use of a circulating supply system, the speed of microbial transmission will be accelerated, endangering the health of plant roots.
[0006] Therefore, a soilless culture agricultural planting cabinet and a method for using the same are proposed. Summary of the invention
[0007] 1. Technical issues to be solved
[0008] In view of the problems existing in the prior art, the purpose of the present invention is to provide a soilless agricultural planting cabinet and a method of using the same. By setting up a real-time microbial detection mechanism, the nutrient solution in the cultivation system can be automatically and regularly tested for microbial reproduction. When the microbial reproduction amount exceeds the standard, an early warning can be given in time, and the staff can be notified to replace the nutrient solution to prevent the microorganisms from corroding the plant roots through the circulation system and endangering the health of the plants.
[0009] 2. Technical solution
[0010] To solve the above problems, the present invention adopts the following technical solutions.
[0011] A soilless cultivation agricultural planting cabinet comprises a cabinet body, a nutrient solution tank is arranged at the lower end of the cabinet body; evenly distributed cultivation troughs are fixedly connected to the middle position of the cabinet body; light sources are arranged on the lower surfaces of the cultivation troughs; a liquid outlet pipe is fixedly connected to one side of the nutrient solution tank; a liquid return pipe is arranged on the other side of the nutrient solution tank, wherein the liquid outlet pipe and the liquid return pipe are interconnected with each layer of the cultivation troughs; a microorganism real-time detection mechanism for detecting the reproduction of microorganisms in the nutrient solution is also arranged at the connection position between the liquid return pipe and the nutrient solution tank;
[0012] The real-time microbial detection mechanism includes an outer shell body fixedly connected to one side of the nutrient solution tank; an inner shell body is provided at the upper inner end of the outer shell body; the lower end of the liquid return pipe extends into the inner shell body and is fixedly connected to the liquid collection box; a reflux pipe is provided on the outer surface of one side of the liquid collection box, and one end of the reflux pipe passes through the inner shell body, the outer shell body and is connected to the nutrient solution tank; a sample tube is provided on the outer surface of the other side of the liquid collection box, and the lower end of the sample tube passes through the inner shell body; a water sampling mechanism is also provided inside the liquid collection box; an ELISA instrument is provided on the lower surface of the liquid collection box near the sample tube; an automatic membrane replacement mechanism is provided on one side of the inner bottom of the outer shell body; a waste liquid outlet is opened on the inner bottom of the outer shell body near the side of the automatic membrane replacement mechanism; a nucleic acid dye storage box for dye addition is also provided on the outer surface of one side of the outer shell body, and the lower end of the nucleic acid dye storage box is extended to the inside of the outer shell body by a pipeline.
[0013] Furthermore, the hole film automatic replacement mechanism includes a mounting frame fixedly connected to one side of the bottom of the outer shell; a hole film storage mechanism is provided on one side of the outer surface of the mounting frame; the upper end of the mounting frame is fixedly connected to the rotating mechanical arm; the upper end of the rotating mechanical arm is fixedly connected to the vacuum generator; one side of the lower surface of the rotating mechanical arm is fixedly connected to the No. 1 telescopic rod, and the lower end of the No. 1 telescopic rod is fixedly connected to the electromagnet ring; the position of the lower surface of the rotating mechanical arm away from the No. 1 telescopic rod is fixedly connected to the No. 2 telescopic rod, and the lower end of the No. 2 telescopic rod is fixedly connected to the vacuum suction cup;
[0014] A waste hole film collection box is fixedly connected to one side of the inner bottom of the outer shell body near the mounting frame; a rotating mechanism is provided on one side of the inner bottom of the outer shell body near the waste hole film collection box; the upper end of the rotating mechanism is rotatably connected to the support frame; one end of the support frame is fixedly connected to the fixed magnet ring; the upper end of the fixed magnet ring is connected to the film pressing iron ring through magnetic field adsorption, and when the hole film is replaced, the center positions of the film pressing iron ring, the fixed magnet ring and the electromagnet ring are all in the same straight line.
[0015] Furthermore, the porous membrane storage mechanism comprises a storage box fixedly connected to the outer surface of one side of the mounting frame; the interior of the storage box is provided with evenly distributed retaining frames; and the upper end of each layer of retaining frames is provided with a microporous filter membrane.
[0016] Furthermore, the rotating mechanism includes a rotating shell fixedly connected to the bottom of the outer shell; one side outer surface of the rotating shell is fixedly connected to the motor, and the output end of the motor extends to the inside of the rotating shell and is fixedly connected to the worm; one side of the worm is meshed with a worm wheel; the lower end of the support frame passes through the rotating shell and is fixedly connected to the middle position of the upper surface of the worm wheel.
[0017] Furthermore, the water sampling mechanism includes a partition shell arranged inside the liquid collection box; an opening is provided at the top of the partition shell; a filtering mechanism is provided at a position near the opening at the upper end of the partition shell; a partition is fixedly connected to the middle position inside the partition shell; a No. 3 telescopic rod is fixedly connected to the bottom of the partition shell; the upper end of the No. 3 telescopic rod is fixedly connected to a hollow tube; the hollow tube passes through the partition, and a liquid inlet distributed in a ring shape is opened on the upper side of the outer circumferential surface of the hollow tube, and a liquid outlet distributed in a ring shape is opened on the lower side of the outer circumferential surface of the hollow tube; the upper end of the hollow tube is fixedly connected to the upper cover body; one end of the sample tube extends to the inside of the partition shell, and a liquid flow meter is provided on the sample tube.
[0018] Furthermore, the filtering mechanism includes a disassembly mechanism fixedly connected to the upper end of the interior of the partition shell; the lower end of the disassembly mechanism is fixedly connected to the filter plate; and the inner upper end of the filter plate is fixedly connected to the liquid inlet pipe.
[0019] Furthermore, the disassembly mechanism includes a mounting ring fixedly connected to the upper end of the partition shell and fixing columns symmetrically fixed to both sides of the outer circumferential surface of the filter plate; the outer circumferential surface of the mounting ring is symmetrically provided with two fixing grooves; the filter plate is fixedly connected to the mounting ring through the fixing columns and the fixing grooves.
[0020] Furthermore, a sealing ring made of rubber material is provided on the outer circumferential surface of the upper cover body; and a sealing stuffing box is also provided at the connection position between the hollow tube and the partition.
[0021] Furthermore, ultraviolet lamps are provided on one side of the inner surface of the outer shell and the liquid collection box.
[0022] 3. Beneficial effects
[0023] Compared with the prior art, the advantages of the present invention are:
[0024] (1) The present invention provides a real-time microbial detection mechanism, which can automatically and regularly detect the microbial reproduction status of the nutrient solution in the cultivation system. When the microbial reproduction amount exceeds the standard, an early warning can be issued in time, and the staff can be notified to replace the nutrient solution to prevent the microorganisms from corroding the plant roots through the circulation system and endangering the health of the plants. The real-time microbial detection mechanism provided in the present invention has a high degree of automation and can automatically detect the microbial reproduction status without manual operation, thereby reducing the labor detection cost. The real-time microbial detection mechanism can obtain the detection results within a few minutes, and has a high detection system efficiency.
[0025] (2) The present invention is capable of automatically replacing the microporous filter membrane by setting up an automatic pore membrane replacement mechanism without opening the outer shell. The replacement operation is simple and efficient, which improves the degree of automation of the real-time microbial detection mechanism, can meet the needs of high-frequency microbial reproduction detection, and reduces labor costs and labor intensity of staff.
[0026] (3) The present invention can automatically and quantitatively sample the nutrient solution by setting up a water sampling mechanism, and cooperate with the automatic pore membrane replacement mechanism to realize the fully automatic operation of the microbial real-time detection mechanism without the need for human intervention. By setting up a filtering mechanism, impurities in the nutrient solution can be filtered to prevent affecting the test results and clogging the liquid flow channel. It should be noted that the liquid inlet pipe and the filter plate are fixedly connected to the partition shell by a disassembly mechanism, which can avoid the common problem of bolt connections being easily corroded in liquids, extend the service life, and make disassembly and assembly more convenient. BRIEF DESCRIPTION OF THE DRAWINGS
[0027] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;
[0028] Figure 2 It is a schematic diagram of the overall bottom-up structure of the present invention;
[0029] Figure 3 It is a schematic cross-sectional structure diagram of the real-time microbial detection mechanism of the present invention;
[0030] Figure 4 It is a structural schematic diagram of the automatic hole film replacement mechanism of the present invention;
[0031] Figure 5 It is a structural schematic diagram of the porous film storage mechanism of the present invention;
[0032] Figure 6 It is a schematic diagram of a top cross-sectional structure of the rotating mechanism of the present invention;
[0033] Figure 7 For the present invention Figure 3 A schematic diagram of the enlarged structure of the water sampling mechanism;
[0034] Figure 8 It is a three-dimensional structural schematic diagram of the filtering mechanism of the present invention;
[0035] Fig. 9 It is a schematic diagram of the process of the present invention.
[0036] Description of the numbers in the figure:
[0037] 1. Cabinet; 2. Water sampling mechanism; 21. Partition shell; 22. Filter mechanism; 221. Liquid inlet pipe; 222. Filter plate; 223. Fixed column; 224. Fixed groove; 225. Mounting ring; 23. Hollow tube; 24. Upper cover; 25. Sealing ring; 26. Liquid inlet; 27. Liquid outlet; 28. Sealing stuffing box; 29. No. 3 telescopic rod; 210. Partition plate; 211. Liquid flow meter; 3. Microbial real-time detection mechanism; 4. Liquid return pipe; 5. Cultivation tank; 6. Liquid outlet pipe; 7. Nutrient solution tank; 8. Light source; 9. Automatic replacement mechanism for porous membrane; 91. Mounting frame; 92. Porous membrane storage mechanism; 921. Storage box ; 922, microporous filter membrane; 923, retaining frame; 93, telescopic rod No. 1; 94, rotating mechanical arm; 95, vacuum generator; 96, telescopic rod No. 2; 97, electromagnet ring; 98, vacuum suction cup; 99, rotating mechanism; 991, rotating shell; 992, worm gear; 993, motor; 994, worm; 910, supporting frame; 911, film pressing iron ring; 912, fixed magnet ring; 913, waste pore membrane collection box; 10, outer shell; 11, ultraviolet lamp; 12, reflux pipe; 13, inner shell; 14, liquid collection box; 15, sample tube; 16, microplate reader; 17, waste liquid outlet; 18, nucleic acid stain storage box. DETAILED DESCRIPTION
[0038] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention; it is obvious that the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments, and all other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making creative work are within the scope of protection of the present invention.
[0039] Embodiment 1:
[0040] See also Figures 1 to 9 A soilless cultivation agricultural planting cabinet comprises a cabinet body 1, a nutrient solution tank 7 is provided at the lower end of the cabinet body 1; evenly distributed cultivation troughs 5 are fixedly connected to the middle position of the cabinet body 1; a light source 8 is provided on the lower surface of the cultivation troughs 5; a liquid outlet pipe 6 is fixedly connected to one side of the nutrient solution tank 7; a liquid return pipe 4 is provided on the other side of the nutrient solution tank 7, wherein the liquid outlet pipe 6 and the liquid return pipe 4 are interconnected with each layer of the cultivation troughs 5; a microbial real-time detection mechanism 3 for detecting the reproduction of microorganisms in the nutrient solution is also provided at the connection position between the liquid return pipe 4 and the nutrient solution tank 7;
[0041] The microorganism real-time detection mechanism 3 includes an outer shell 10 fixedly connected to one side of the nutrient solution tank 7; an inner shell 13 is provided at the upper end of the inner shell 10; the lower end of the liquid return pipe 4 extends into the inner shell 13 and is fixedly connected to the liquid collection box 14; a return pipe 12 is provided on the outer surface of one side of the liquid collection box 14, and one end of the return pipe 12 passes through the inner shell 13 and the outer shell 10 and is connected to the nutrient solution tank 7; a sample tube 15 is provided on the outer surface of the other side of the liquid collection box 14, and the lower end of the sample tube 15 passes through the inner shell 13. The liquid collection box 14 is also provided with a water sampling mechanism 2; a microplate reader 16 is provided on the lower surface of the liquid collection box 14 near the sample tube 15; an automatic membrane replacement mechanism 9 is provided on one side of the inner bottom of the outer shell 10; a waste liquid outlet 17 is provided on one side of the inner bottom of the outer shell 10 near the automatic membrane replacement mechanism 9; a nucleic acid stain storage box 18 for adding a stain is also provided on the outer surface of one side of the outer shell 10, and the lower end of the nucleic acid stain storage box 18 is extended to the inside of the outer shell 10 by a pipeline.
[0042] In actual use, the microorganisms present in the nutrient solution preparation process will multiply a large number of microorganisms and bacteria in hot and humid weather, and the use of a circulating supply system will accelerate the speed of microbial infection and endanger the health of plant roots;
[0043] The present invention solves this technical problem by setting up a real-time microbial detection mechanism 3. When the real-time microbial detection mechanism 3 is working, first, the nutrient solution refluxed through the return pipe 4 enters the liquid collection box 14, and the nutrient solution is sampled by the water body sampling mechanism 2. The remaining nutrient solution flows back to the nutrient solution tank 7 through the return pipe 12. The water body sampling mechanism 2 drips the sampled nutrient solution from the sample tube 15 to the automatic porous membrane replacement mechanism 9, and uses the filter membrane fixed on the automatic porous membrane replacement mechanism 9 to perform microporous filtration on the nutrient solution sample, so that the microorganisms remain on the filter membrane, and then uses the nucleic acid dye storage box 18 to drip an appropriate amount of nucleic acid dye onto the filtered microporous filter membrane 922. The coloring agent is kept away from light for five minutes to facilitate staining of the microorganisms. After the microorganisms are stained, a 470nm-510nm laser is used to irradiate the microorganisms so that the microorganisms produce fluorescence emission. Then, the microorganisms on the microporous filter membrane 922 are measured for fluorescence intensity using an ELISA instrument 16. Finally, the fluorescence intensity parameter is brought into the standard linear regression equation of the total number of bacteria and the fluorescence intensity. After calculation, the total number of microbial bacteria is obtained, thereby realizing the detection of the microbial reproduction in the nutrient solution. After the detection is completed, the used microporous filter membrane 922 is automatically replaced by the automatic membrane replacement mechanism 9 for the next detection;
[0044] The present invention is capable of automatically and regularly detecting the microbial reproduction conditions of the nutrient solution in the cultivation system by setting up a real-time microbial detection mechanism 3, and can promptly issue an early warning when the microbial reproduction amount exceeds the standard, and notify the staff to replace the nutrient solution to prevent the microorganisms from corroding the plant roots through the circulation system and endangering the health of the plants. The real-time microbial detection mechanism 3 provided in the present invention has a high degree of automation and can automatically detect the microbial reproduction conditions without the need for manual operation, thereby reducing the labor detection cost. The real-time microbial detection mechanism 3 can obtain the detection results within a few minutes, and the detection efficiency is relatively high.
[0045] like Figure 4 As shown, the hole film automatic replacement mechanism 9 includes a mounting frame 91 fixedly connected to one side of the bottom of the outer shell 10; a hole film storage mechanism 92 is provided on one side of the outer surface of the mounting frame 91; the upper end of the mounting frame 91 is fixedly connected to a rotating mechanical arm 94; the upper end of the rotating mechanical arm 94 is fixedly connected to a vacuum generator 95; one side of the lower surface of the rotating mechanical arm 94 is fixedly connected to a first telescopic rod 93, and the lower end of the first telescopic rod 93 is fixedly connected to an electromagnet ring 97; the lower surface of the rotating mechanical arm 94 is fixedly connected to a second telescopic rod 96 away from the first telescopic rod 93, and the lower end of the second telescopic rod 96 is fixedly connected to a vacuum suction cup 98;
[0046] A waste hole film collection box 913 is fixedly connected to one side of the bottom inner side of the outer shell 10 near the mounting frame 91; a rotating mechanism 99 is provided on one side of the bottom inner side of the outer shell 10 near the waste hole film collection box 913; the upper end of the rotating mechanism 99 is rotatably connected to the support frame 910; one end of the support frame 910 is fixedly connected to the fixed magnet ring 912; the upper end of the fixed magnet ring 912 is connected to the film pressing iron ring 911 through magnetic field adsorption, and when the hole film is replaced, the center positions of the film pressing iron ring 911, the fixed magnet ring 912, and the electromagnet ring 97 are all in the same straight line.
[0047] like Figure 5 As shown, the porous membrane storage mechanism 92 includes a storage box 921 fixedly connected to the outer surface of one side of the mounting frame 91; the interior of the storage box 921 is provided with evenly distributed retaining frames 923; and the upper end of each layer of retaining frames 923 is provided with a microporous filter membrane 922.
[0048] like Figure 6 As shown, the rotating mechanism 99 includes a rotating shell 991 fixedly connected to the inner bottom of the outer shell 10; the outer surface of one side of the rotating shell 991 is fixedly connected to the motor 993, and the output end of the motor 993 extends to the inside of the rotating shell 991 and is fixedly connected to the worm 994; one side of the worm 994 is meshedly connected with a worm wheel 992; the lower end of the support frame 910 passes through the rotating shell 991 and is fixedly connected to the middle position of the upper surface of the worm wheel 992.
[0049] After the microbial real-time detection mechanism 3 performs detection, the used microporous filter membrane 922 needs to be replaced for the next detection. The entire microbial real-time detection mechanism 3 is arranged inside the outer shell 10. When replacing, the outer shell 10 needs to be opened, which is cumbersome and inefficient. In addition, the microbial real-time detection mechanism 3 of the present invention is used for real-time detection, and its detection frequency is relatively high. In hot weather, sampling and detection may even be performed at a frequency of several minutes. Manually replacing the microporous filter membrane 922 at a high frequency also requires high labor costs.
[0050] The present invention solves this technical problem by setting up an automatic pore membrane replacement mechanism 9. When the automatic pore membrane replacement mechanism 9 is working, the rotating mechanism 99 works first, and the motor 993 starts to drive the worm 994 connected thereto to rotate, and the worm 994 is meshed and connected with the worm wheel 992, thereby driving the worm wheel 992 to rotate, and the lower end of the support frame 910 is fixedly connected to the worm wheel 992, so it drives the support frame 910 to rotate. It should be noted here that the setting of the rotating mechanism 99 can not only achieve the rotation effect, but also because the worm wheel 992 and the worm 994 are a reduction system, it can reduce the transmission efficiency of the motor 993 and improve the rotation stability; after the rotating mechanism 99 drives the support frame 910 to rotate to 180 degrees, the microporous filter membrane 9 22 is rotated from the position just above the waste liquid outlet 17 to the position just above the waste hole membrane collection box 913. At this time, the No. 1 telescopic rod 93 is controlled to extend, driving the electromagnet ring 97 to descend until it is close to the film pressing iron ring 911, and the electromagnet ring 97 is energized. The electromagnet ring 97 generates a magnetic field due to the electromagnetic effect, and adsorbs the film pressing iron ring 911. At this time, the film pressing iron ring 911 is simultaneously adsorbed by the fixed magnet ring 912 and the electromagnet ring 97. Controlling the current intensity can control the magnetic field intensity of the electromagnet ring 97, so that the adsorption force of the electromagnet ring 97 on the film pressing iron ring 911 is greater than the adsorption force of the fixed magnet ring 912 on the film pressing iron ring 911, and then the No. 1 telescopic rod 93 is controlled to shorten, and then the electromagnet ring 97 uses strong adsorption force to bring the film pressing iron ring 911 away from the surface of the fixed magnet ring 912, and the microporous filter membrane 922 that loses the fixation of the film pressing iron ring 911 is no longer subjected to the fixing force. At this time, the second telescopic rod 96 is controlled to extend to drive the vacuum suction cup 98 to descend. The diameter of the vacuum suction cup 98 is smaller than the diameter of the fixed magnet ring 912, so the microporous filter membrane 922 can be pushed out and dropped into the waste microporous membrane collection box 913 during the descent process. At this time, the disassembly of the old microporous filter membrane 922 is completed, and then, the rotating mechanical arm 94 works to drive the vacuum suction cup 98 to rotate to the top of the microporous filter storage mechanism 92, and the vacuum generator 95 is turned on. The vacuum suction cup 98 generates an adsorption force to the new microporous filter membrane 922 inside the microporous filter storage mechanism 92. After the film is adsorbed and fixed, the rotating mechanical arm 94 rotates again, so that the vacuum suction cup 98 moves to the top of the fixed magnet ring 912, the second telescopic rod 96 is controlled to extend, and then the vacuum generator 95 is controlled to stop working, the adsorption force of the vacuum suction cup 98 disappears, and the new microporous filter membrane 922 stays on the upper surface of the fixed magnet ring 912 at this time. Finally, the first telescopic rod 93 is extended, driving the electromagnet ring 97 and the film pressing iron ring 911 to descend until the film pressing iron ring 911 is adsorbed with the fixed magnet ring 912 again. At this time, the electromagnet ring 97 is powered off, and the new microporous filter membrane 922 is clamped and fixed by the film pressing iron ring 911 and the fixed magnet ring 912, thereby completing the automatic replacement of the microporous filter membrane 922;It should also be noted here that the microporous membrane storage mechanism 92 is provided with a uniformly distributed holder 923 to layer the microporous membranes 922, so as to prevent the vacuum suction cup 98 from adsorbing multiple microporous membranes 922 at one time, thereby affecting the test results;
[0051] The present invention is capable of automatically replacing the microporous filter membrane 922 by providing an automatic pore membrane replacement mechanism 9, without opening the outer shell 10. The replacement operation is simple and efficient, thereby improving the degree of automation of the real-time microbial detection mechanism 3, being able to meet the needs of high-frequency microbial reproduction detection, and reducing labor costs and the labor intensity of staff.
[0052] like Figure 7 As shown, the water sampling mechanism 2 includes a partition shell 21 arranged inside the liquid collection box 14; an opening is provided at the top of the partition shell 21; a filtering mechanism 22 is provided at a position near the opening at the upper end of the partition shell 21; a partition plate 210 is fixedly connected to the middle position inside the partition shell 21; a No. 3 telescopic rod 29 is fixedly connected to the bottom of the partition shell 21; the upper end of the No. 3 telescopic rod 29 is fixedly connected to a hollow tube 23; the hollow tube 23 passes through the partition plate 210, and a liquid inlet 26 distributed in an annular manner is provided on the upper side of the outer circumferential surface of the hollow tube 23, and a liquid outlet 27 distributed in an annular manner is provided on the lower side of the outer circumferential surface of the hollow tube 23; the upper end of the hollow tube 23 is fixedly connected to the upper cover body 24; one end of the sample tube 15 extends to the inside of the partition shell 21, and a liquid flow meter 211 is provided on the sample tube 15.
[0053] like Figure 8 As shown, the filter mechanism 22 includes a disassembly mechanism fixedly connected to the upper end of the interior of the partition shell 21 ; the lower end of the disassembly mechanism is fixedly connected to the filter plate 222 ; the inner upper end of the filter plate 222 is fixedly connected to the liquid inlet pipe 221 .
[0054] like Figure 8 As shown, the disassembly mechanism includes a mounting ring 225 fixedly connected to the upper end of the interior of the partition shell 21 and fixing columns 223 symmetrically fixedly connected to both sides of the outer circumferential surface of the filter plate 222; the outer circumferential surface of the mounting ring 225 is symmetrically provided with two fixing grooves 224; the filter plate 222 is fixedly connected to the mounting ring 225 via the fixing columns 223 and the fixing grooves 224.
[0055] like Figure 7 As shown, a rubber sealing ring 25 is disposed on the outer circumferential surface of the upper cover 24 ; a sealing stuffing box 28 is also disposed at the connection position between the hollow tube 23 and the partition plate 210 .
[0056] When the microorganism real-time detection mechanism 3 is working, it is necessary to use the water sampling mechanism 2 to sample the nutrient solution. When the water sampling mechanism 2 is working, first, the nutrient solution in the liquid collection box 14 enters the filter mechanism 22 from the opening at the upper end of the partition shell 21 to filter the impurities in the nutrient solution to prevent affecting the detection results and blocking the liquid flow channel. The filter mechanism 22 is composed of a liquid inlet pipe 221, a filter plate 222 and a disassembly mechanism. The nutrient solution entering the opening enters through the liquid inlet pipe 221, and then the liquid level rises and flows into the filter plate 222. Inside the partition shell 21, the filter plate 222 filters the impurities in the nutrient solution. It should be noted that the liquid inlet pipe 221 and the filter plate 222 are fixedly connected to the partition shell 21 through a disassembly mechanism, which can avoid the problem of common bolt connections being easily corroded in the liquid, prolonging the service life, and making disassembly and assembly more convenient. During installation, it is only necessary to align the fixing column 223 with the fixing groove 224 and insert it, and then rotate the filter plate 222 and the liquid inlet pipe 221 combination so that the fixing column 223 moves to the lowest position of the fixing groove 224 to achieve the rotation. The fixing of the rotating filter plate 222 and the mounting ring 225 facilitates the replacement and cleaning of the rotating filter plate 222; after the nutrient solution is filtered by the filtering mechanism 22 to remove impurities, it flows to the upper end of the interior of the partition shell 21. At this time, the third telescopic rod 29 works to drive the hollow tube 23 to move upward, and the liquid inlet 26 opened at the upper end of the hollow tube 23 is no longer blocked by the partition plate 210, so that the nutrient solution at the upper end of the interior of the partition shell 21 flows from the liquid inlet 26 into the hollow tube 23, and then flows from the liquid outlet 27 at the lower end of the hollow tube 23 to the lower end of the interior of the partition shell 21, and then flows through the liquid outlet 27 at the lower end of the hollow tube 23 to the lower end of the interior of the partition shell 21. The sample tube 15 falls onto the microporous filter membrane 922, and the liquid flow meter 211 can detect the sampling amount, which is convenient for controlling the number of samples. When the sampling amount is reached, the third telescopic rod 29 is shortened, driving the hollow tube 23 to descend, and the liquid inlet 26 is blocked by the partition 210 again to achieve isolation of the nutrient solution and complete sampling. It should be noted that a rubber sealing ring 25 is provided on the outer circumferential surface of the upper cover body 24, and a sealing stuffing box 28 is also provided at the connection position of the hollow tube 23 and the partition 210, thereby improving the sealing effect of the partition 210;
[0057] The present invention can automatically and quantitatively sample the nutrient solution by setting up a water sampling mechanism 2, and cooperate with the automatic pore membrane replacement mechanism 9 to realize the fully automatic operation of the microbial real-time detection mechanism 3 without the need for manual intervention. By setting up a filtering mechanism 22, impurities in the nutrient solution can be filtered to prevent affecting the test results and clogging the liquid flow channel. It should be noted that the liquid inlet pipe 221 and the filter plate 222 are fixedly connected to the partition shell 21 by a disassembly mechanism, which can avoid the common problem of bolt connections being easily corroded in liquids, extend the service life, and make disassembly and assembly more convenient.
[0058] like Figure 3As shown, an ultraviolet lamp 11 is provided on one side of the inner surface of the outer shell 10 and the liquid collection box 14 to disinfect and sterilize the internal equipment of the outer shell 10 and the liquid collection box 14 to avoid adverse effects on the detection accuracy of the real-time microbial detection mechanism 3.
[0059] A method for using a soilless cultivation agricultural planting cabinet, such as Fig. 9 As shown, the following steps are included:
[0060] S1: Plant crop seeds inside each layer of cultivation trough 5, control the pump body inside the nutrient solution tank 7 to pump the nutrient solution out from the outlet pipe 6 and into the nutrient solution tank inside the cultivation trough 5; then the nutrient solution flows out from the nutrient solution tank and returns to the nutrient solution tank 7 through the return pipe 4, completing the nutrient solution irrigation of the crop seeds;
[0061] S2: Based on S1, the microbial real-time detection mechanism 3 can be used to perform real-time detection on the refluxed nutrient solution, so as to understand the current microbial reproduction situation in the nutrient solution. The work of the microbial real-time detection mechanism 3 is divided into the following steps;
[0062] S3: sampling the nutrient solution in the liquid return pipe 4 using the water sampling mechanism 2, and filtering the sample using the microporous filter membrane 922;
[0063] S4: Based on S3, a proper amount of nucleic acid stain is dripped onto the filtered microporous filter membrane 922 using the nucleic acid stain storage box 18, and the mixture is allowed to stand for five minutes away from light to stain the microorganisms;
[0064] S5: Use the microplate reader 16 to measure the fluorescence intensity of the microorganisms on the microporous filter membrane 922, and then bring the parameter into the linear regression equation of the total number of bacteria and the fluorescence intensity, and after calculation, the total number of microbial bacteria is obtained, thereby realizing the detection of the microbial reproduction in the nutrient solution;
[0065] S6: After the detection is completed, the used microporous filter membrane 922 is automatically replaced by the automatic membrane replacement mechanism 9 so as to facilitate the next detection.
[0066] The above is only a preferred specific implementation of the present invention; however, the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solution and its improved conception within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.
Claims
1. A soilless agricultural cultivation cabinet, comprising a cabinet body (1), characterized in that: A nutrient solution tank (7) is provided at the lower end of the cabinet (1); evenly distributed cultivation troughs (5) are fixedly connected to the middle position of the cabinet (1); a light source (8) is provided on the lower surface of each cultivation trough (5); a liquid outlet pipe (6) is fixedly connected to one side of the nutrient solution tank (7); a liquid return pipe (4) is provided on the other side of the nutrient solution tank (7), wherein the liquid outlet pipe (6) and the liquid return pipe (4) are interconnected with each layer of cultivation troughs (5); a microorganism real-time detection mechanism (3) for detecting the growth status of microorganisms in the nutrient solution is also provided at the connection position between the liquid return pipe (4) and the nutrient solution tank (7); The microorganism real-time detection mechanism (3) comprises an outer shell (10) fixedly connected to one side of a nutrient solution tank (7); an inner shell (13) is provided at the upper end of the inner part of the outer shell (10); the lower end of the liquid return pipe (4) extends into the inner shell (13) and is fixedly connected to a liquid collection box (14); a return pipe (12) is provided on the outer surface of one side of the liquid collection box (14), and one end of the return pipe (12) passes through the inner shell (13) and the outer shell (10) and is connected to the nutrient solution tank (7); a sample tube (15) is provided on the outer surface of the other side of the liquid collection box (14), and the lower end of the sample tube (15) passes through the inner shell (13) and is connected to the nutrient solution tank (7); 13) is arranged; a water sampling mechanism (2) is also arranged inside the liquid collection box (14); an ELISA instrument (16) is arranged at a position near the sample tube (15) on the lower surface of the liquid collection box (14); an automatic membrane replacement mechanism (9) is arranged on one side of the inner bottom of the outer shell (10); a waste liquid outlet (17) is opened at a position near the automatic membrane replacement mechanism (9) on one side of the inner bottom of the outer shell (10); a nucleic acid dye storage box (18) for adding a dye is also arranged on the outer surface of one side of the outer shell (10), and the lower end of the nucleic acid dye storage box (18) is extended to the inside of the outer shell (10) by a pipeline; The water sampling mechanism (2) comprises a partition shell (21) arranged inside a liquid collection box (14); an opening is provided at the top of the partition shell (21); a filtering mechanism (22) is provided at a position near the opening at the upper end of the partition shell (21); a partition plate (210) is fixedly connected to the middle position inside the partition shell (21); a third telescopic rod (29) is fixedly connected to the bottom of the partition shell (21); the upper end of the third telescopic rod (29) is fixedly connected to a hollow tube (23); the hollow tube (23) passes through the partition plate (210), and a liquid inlet (26) distributed in an annular manner is provided on the upper side of the outer circumferential surface of the hollow tube (23), and a liquid outlet (27) distributed in an annular manner is provided on the lower side of the outer circumferential surface of the hollow tube (23); the upper end of the hollow tube (23) is fixedly connected to an upper cover body (24); one end of the sample tube (15) extends into the interior of the partition shell (21), and a liquid flow meter (211) is provided on the sample tube (15); The filtering mechanism (22) comprises a disassembly mechanism fixedly connected to the upper end of the interior of the partition shell (21); the lower end of the disassembly mechanism is fixedly connected to a filter plate (222); and the upper end of the interior of the filter plate (222) is fixedly connected to a liquid inlet pipe (221).
2. The soilless agricultural planting cabinet according to claim 1, characterized in that: The automatic hole film replacement mechanism (9) comprises a mounting frame (91) fixedly connected to one side of the bottom of the outer shell (10); a hole film storage mechanism (92) is provided on one side of the outer surface of the mounting frame (91); the upper end of the mounting frame (91) is fixedly connected to a rotating mechanical arm (94); the upper end of the rotating mechanical arm (94) is fixedly connected to a vacuum generator (95); one side of the lower surface of the rotating mechanical arm (94) is fixedly connected to a first telescopic rod (93), and the lower end of the first telescopic rod (93) is fixedly connected to an electromagnet ring (97); the lower surface of the rotating mechanical arm (94) is fixedly connected to a second telescopic rod (96) at a position away from the first telescopic rod (93), and the lower end of the second telescopic rod (96) is fixedly connected to a vacuum suction cup (98); A waste hole film collection box (913) is fixedly connected to one side of the inner bottom of the outer shell (10) close to the mounting frame (91); a rotating mechanism (99) is provided on one side of the inner bottom of the outer shell (10) close to the waste hole film collection box (913); the upper end of the rotating mechanism (99) is rotatably connected to the support frame (910); one end of the support frame (910) is fixedly connected to the fixed magnet ring (912); the upper end of the fixed magnet ring (912) is connected to the film pressing iron ring (911) through magnetic field adsorption, and when the hole film is replaced, the center positions of the film pressing iron ring (911), the fixed magnet ring (912) and the electromagnet ring (97) are all on the same straight line.
3. The soilless agricultural planting cabinet according to claim 2, characterized in that: The porous membrane storage mechanism (92) comprises a storage box (921) fixedly connected to the outer surface of one side of the mounting frame (91); evenly distributed retaining frames (923) are provided inside the storage box (921); and a microporous filter membrane (922) is provided at the upper end of each layer of the retaining frames (923).
4. The soilless agricultural cultivation cabinet according to claim 2, characterized in that: The rotating mechanism (99) comprises a rotating shell (991) fixedly connected to the inner bottom of the outer shell (10); a motor (993) is fixedly connected to an outer surface of one side of the rotating shell (991), and an output end of the motor (993) extends into the interior of the rotating shell (991) and is fixedly connected to a worm (994); a worm wheel (992) is meshedly connected to one side of the worm wheel (994); and the lower end of the support frame (910) passes through the rotating shell (991) and is fixedly connected to a middle position of an upper surface of the worm wheel (992).
5. The soilless cultivation agricultural planting cabinet according to claim 1, characterized in that: The disassembly mechanism comprises a mounting ring (225) fixedly connected to the upper end of the interior of the partition shell (21) and fixing columns (223) symmetrically fixedly connected to both sides of the outer circumferential surface of the filter plate (222); the outer circumferential surface of the mounting ring (225) is symmetrically provided with two fixing grooves (224); the filter plate (222) is fixedly connected to the mounting ring (225) via the fixing columns (223) and the fixing grooves (224).
6. The soilless cultivation agricultural planting cabinet according to claim 1, characterized in that: The outer circumferential surface of the upper cover body (24) is provided with a sealing ring (25) made of rubber material; and a sealing stuffing box (28) is also provided at the connection position between the hollow tube (23) and the partition plate (210).
7. The soilless cultivation agricultural planting cabinet according to claim 1, characterized in that: An ultraviolet lamp (11) is provided on one side of the inner surface of the outer shell (10) and the liquid collection box (14).
Citation Information
Patent Citations
Method for measuring density of bacteria in water sample
CN106153591A
Microorganism regular sampling detection device and sampling method
CN112592814A