A repellent for adults of spruce beetles and its application

Abstract

A repellent for adults of spruce beetles and its application, belong to the technical field of using chemical information substances to repel pests. A repellent for adults of spruce beetles of the present invention comprises the following components in parts by weight: 1-100 parts of 1,8-cineole and 1-100 parts of 3-carene. The repellent for adults of spruce beetles of the present invention can be hung around the trunk of the host tree of spruce beetles to repel the damage caused by the adults of spruce beetles boring into the trees. The repellent of the present invention can last up to 30-120 days, and the repellent for adults of spruce beetles of the present invention is environmentally friendly, reduces the use of chemical pesticides, is particularly suitable for promotion and application in the forest, and has good economic, social and ecological benefits and broad industrialization prospects.

Description

Technical Field

[0001] The invention belongs to the technical field of using chemical information substances to repel pests, and specifically relates to a repellent for adults of spruce beetles and an application thereof. Background Art

[0002] The spruce eight-toothed beetle Ips typographus Linnaeus belongs to the Coleoptera, Scolytidae, and Ips genus. Its original host abroad is the Norway spruce (Picea abies (L.)), one of the most important economic tree species in Europe. In China, it mainly harms red-skinned spruce Picea koraiensis, fish-scale spruce P. jezoensis var. microsperma, and snow-capped spruce P. schrenkiana. The spruce eight-toothed beetle is widely distributed throughout the Palearctic region. It is an important trunk-boring pest in the spruce ecosystem and a secondary pest. It generally harms natural spruce forests, especially mature and over-mature spruce forests. Under suitable conditions or when the pest is serious, it can also directly cause damage to healthy standing trees. The serious damage caused by the spruce eight-toothed beetle has caused large-scale death of trees, bringing huge ecological and economic losses to the local forest ecological environment and forestry production. It is considered to be the most destructive species in coniferous forests to date.

[0003] Spruce relies on structural defense and induced defense to repel the invading spruce beetle. Its first line of defense against spruce beetles is the bark. Producing a large number of defensive chemicals to deal with the infestation of spruce beetles will help them survive in the long term. Spruce bark uses its own physical properties and chemical toxicity to prevent the invasion of spruce beetles. Once the damage or infection is recognized by spruce, it will stimulate the production of induced defense, including the formation of traumatic resin ducts and polyphenol thin-walled cells, and may increase the concentration of terpenes by 100 times or more. As the concentration increases, it can more effectively inhibit the colonization of spruce beetles. Studies have found that trans-4-thujanol in the bark decreases with the age of the tree. This substance has also been detected in the volatiles of spruce bored by male spruce beetles. During an outbreak of spruce beetles, host trees that were not attacked by spruce beetles released higher amounts of 1,8-cineole than successfully colonized host trees. Under laboratory conditions, both trans-4-thujanol and 1,8-cineole have been shown to inhibit spruce beetle aggregation pheromones.

[0004] Non-host green leaf volatiles can interfere with the response of the spruce beetle to pheromones. The three main components of birch: l-hexanol (l-hexanoal), (Z)-3-hexen-l-ol (Z-3-hexen-l-ol) and (E)-2-hexen-l-ol (E-2-hexen-ol) can cause strong antennal potential responses of the spruce beetle. Adding these three substances to artificially synthesized pheromones can reduce the amount of trapping; cis-2-hexen-l-ol (cis-2-hexen-l-ol), trans-2-hexen-l-ol (trans-2-hexen-ol) at a volume ratio of 0.001 can also cause strong antennal potential responses of the spruce beetle. The results showed that the mixtures of cis-3-hexen-1-ol, cis-3-Hexen-1-ol and 3-Carene, trans-2-hexenal at a volume ratio of 0.01, (-)-isocaryophyllene and 3-Carene, and benzaldehyde at a volume ratio of 0.001 or 0.01 and 3-Carene had a repellent effect on the spruce beetle.

[0005] However, the repellents in the prior art are all composed of multiple components, have poor repellent effects, and are based on laboratory conditions, with few reports on their application in forests. Summary of the invention

[0006] In view of the problems existing in the prior art, the object of the present invention is to design and provide a repellent for adults of the spruce eight-toothed borer and a technical solution for its application in the forest.

[0007] The present invention is specifically implemented through the following technical solutions:

[0008] In one aspect, the present invention provides a repellent for adults of the spruce beetle, comprising the following components in parts by volume:

[0009] 1,8-Cineole 1-100 parts

[0010] 3-Carene 1-100 parts.

[0011] Furthermore, the repellent for adults of the spruce eight-toothed borer comprises the following components in parts by volume:

[0012] 1,8-cineole 5-50 parts

[0013] 3-Carene 5-50 parts.

[0014] Furthermore, the repellent for adults of the spruce eight-toothed borer comprises the following components in parts by volume:

[0015] 1,8-cineole 10-30 parts

[0016] 3-Carene 10-30 parts.

[0017] Furthermore, the volume ratio of 1,8-cineole to 3-carene is 1:0.1-10.

[0018] Furthermore, the volume ratio of 1,8-cineole to 3-carene is 1:1.

[0019] Another aspect of the present invention provides a repellent for adults of the spruce beetle, which is used as a repellent for adults of the spruce beetle.

[0020] The specific steps of the preparation process of the spruce beetle adult repellent of the present invention are as follows:

[0021] 1) Preparation of sustained-release bag: Soak the degradable sustained-release bag in ethanol for 1-3 days, rinse it with distilled water until there is no ethanol smell, and dry it for later use;

[0022] 2) Preparation of repellent: accurately weigh the active ingredients in the formula in milliliters and set aside;

[0023] 3) After adding a medium such as absorbent cotton or gauze into the sustained-release bag, the repellent prepared in step 2) is added dropwise to the attractant carrier prepared in step 1) to prepare the repellent for adult spruce beetles.

[0024] The repellent for adult spruce beetles of the present invention can be hung around the trunk of a host tree of the spruce beetles to repel the damage of the spruce beetles adults boring into the tree. The repellent of the present invention has a lasting effect of 30-120 days, and the repellent for adult spruce beetles of the present invention is environmentally friendly, reduces the use of chemical pesticides, is particularly suitable for promotion and application in forests, and has good economic, social and ecological benefits and broad industrialization prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is the trapping amount of the spruce eight-toothed borer adults in each embodiment. DETAILED DESCRIPTION

[0026] The present invention is further described below with reference to the examples.

[0027] Example 1: Preparation of a spruce beetle repellent (parts by volume)

[0028] Weigh 1 part of 1,8-cineole and 1 part of 3-carene, respectively put them into sustained-release bags filled with absorbent cotton, and thus prepare a repellent for adult spruce beetles.

[0029] Example 2: Preparation of a spruce beetle repellent (parts by volume)

[0030] Weigh 5 parts of 1,8-cineole and 5 parts of 3-carene, and put them into slow-release bags filled with absorbent cotton respectively, so as to prepare a repellent for adults of spruce beetles.

[0031] Example 3: Preparation of a spruce beetle repellent (parts by volume)

[0032] Weigh 10 parts of 1,8-cineole and 10 parts of 3-carene, and put them into slow-release bags filled with absorbent cotton respectively, so as to prepare a repellent for adults of spruce beetles.

[0033] Example 4: Preparation of a spruce beetle repellent (parts by volume)

[0034] Weigh 100 parts of 1,8-cineole and 100 parts of 3-carene, and put them into slow-release bags filled with absorbent cotton respectively, so as to prepare a repellent for adults of spruce beetles.

[0035] Example 5: Preparation of a spruce beetle repellent (parts by volume)

[0036] Weigh 10 parts of 1,8-cineole and 1 part of 3-carene, respectively put them into sustained-release bags filled with absorbent cotton to prepare a repellent for adult spruce beetles.

[0037] Example 6: Preparation of a spruce beetle repellent (parts by volume)

[0038] Weigh 5 parts of 1,8-cineole and 1 part of 3-carene, respectively put them into sustained-release bags filled with absorbent cotton to prepare a repellent for adult spruce beetles.

[0039] Example 7: Preparation of a spruce beetle repellent (parts by volume)

[0040] Weigh 1 part of 1,8-cineole and 5 parts of 3-carene, respectively put them into sustained-release bags filled with absorbent cotton to prepare a repellent for adult spruce beetles.

[0041] Comparative Example 1

[0042] Weigh 1 portion of 1,8-cineole and put it into a sustained-release bag filled with absorbent cotton to prepare a repellent for adults of spruce beetles.

[0043] Comparative Example 2

[0044] Weigh 5 parts of 3-carene and put it into a sustained-release bag filled with absorbent cotton to prepare a repellent for adults of spruce beetles.

[0045] Comparative Example 3

[0046] Weigh 4 grams of myrcene and put it into a sustained-release bag filled with absorbent cotton to prepare a repellent for adult spruce beetles.

[0047] Comparative Example 4

[0048] Weigh 4 grams of nonanal and put it into a sustained-release bag filled with absorbent cotton to prepare a repellent for adult spruce beetles.

[0049] Test Example 1: The spruce beetle repellents prepared in Examples 1, 2, 3, 5 and Comparative Examples 1, 2, 3, 4 were respectively suspended on the connecting rod of the funnel-shaped trap together with an attractant for spruce beetles (60 parts of cis-verbenol, 60 parts of 2-methyl-3-butene-2-ol, 60 parts of α-pinene (+25 / -75), 0.02 parts of 2,6-di-tert-butyl-p-cresol and 0.02 parts of 2-hydroxy-4-methoxybenzophenone, mixed evenly on an oscillator, accurately weighed in milliliters for use, the attractant is an existing product, and its preparation method can be specifically seen in the Chinese invention patent with publication number CN105746511A and application number 201610183543.0). Traps were hung in the 36th forest block of Jingouling Forest Farm, Wangqing Forestry Bureau, with a spacing of 100m between the traps, and each treatment and replicate were randomly distributed. The trap was hung between two trees at a height of about 2m, and the lower part of the trap was about 50cm from the ground. The hanging time was from mid-April to early August. Each treatment had 5 replicates. Data analysis was performed using one-way analysis of variance and Duncan's multiple range test.

[0050] From Table 1 and Figure 1 It can be seen from the survey data that the repellent of the present invention can significantly reduce the trapping effect of the attractant.

[0051] Table 1: The trapping effects of various embodiments of the Wangqing Forestry Bureau of Jilin Province in 2020

[0052] Different embodiments Trapping amount ± standard error Significance of difference Patented attractant formula 4611.4±660.17 a Comparative Example 2 4499.2±761.32 a Comparative Example 3 1874.8±446.56 b Comparative Example 4 1542.2±126.69 b Comparative Example 1 1528.8±67.3 b Example 1 356.8±156.71 c Example 2 297.2±50.41 c Example 3 171.8±21.73 c Example 5 285±71.85 c Blank control 0.4±0.24 c

[0053] Note: Different letters represent significant differences at the 0.05 level.

[0054] Test Example 2: Using the spruce beetle repellent prepared in Example 4, 50 spruce beetles collected in the wild were fed individually indoors for 5 days, starved for 3 hours before the test, and tested with an indoor Y-type olfactometer. The results are shown in Table 2. The test results show that the repellent effect of the attractant formula of the present invention is significantly higher than that of the blank control.

[0055] Table 2 Repellent effect of the present invention (Y-type olfactometer)

[0056]

[0057] χ 2 -test:*P<0.05;**P<0.005.

[0058] Test Example 3: Using the spruce beetle repellent prepared in Example 6, 50 spruce beetles collected in the wild were fed individually indoors for 5 days, starved for 3 hours before the test, and tested with an indoor Y-type olfactometer. The results are shown in Table 3. The test results show that the repellent effect of the attractant formula of the present invention is significantly higher than that of the blank control.

[0059] Table 3 Comparison of repellent effects of the present invention (Y-type olfactometer)

[0060]

[0061] χ 2 -test:*P<0.05;**P<0.005.

[0062] Test Example 4: Using the spruce beetle repellent prepared in Example 7, 50 spruce beetles collected in the wild were fed individually indoors for 5 days, starved for 3 hours before the test, and tested with an indoor Y-type olfactometer. The results are shown in Table 3. The test results show that the repellent effect of the attractant formula of the present invention is significantly higher than that of the blank control.

[0063] Table 4 Comparison of repellent effects of the present invention (Y-type olfactometer)

[0064]

[0065] χ 2 -test:*P<0.05;**P<0.005.

[0066] Test Example 5: Using the spruce beetle repellent prepared in Example 5, 50 spruce beetles collected in the wild were fed individually indoors for 5 days, starved for 3 hours before the test, and tested with an indoor Y-type olfactometer. The results are shown in Table 3. The test results show that the repellent effect of the attractant formula of the present invention is significantly higher than that of the blank control.

[0067] Table 5 Comparison of the repellent effect of the present invention (Y-type olfactometer)

[0068]

[0069] χ 2 -test: *P<0.05; **P<0.005.

Claims

1. A repellent for adults of the spruce beetle, characterized in that It is composed of the following components in parts by volume: 1,8-Cineole 1-100 parts 3-Carene 1-100 parts.

2. A repellent for adults of the spruce beetle as claimed in claim 1, characterized in that It is composed of the following components in parts by volume: 1,8-cineole 5-50 parts 3-Carene 5-50 parts.

3. A repellent for adults of the spruce beetle as claimed in claim 1, characterized in that It is composed of the following components in parts by volume: 1,8-cineole 10-30 parts 3-Carene 10-30 parts.

4. A repellent for adults of the spruce beetle as claimed in claim 1, characterized in that The volume ratio of 1,8-cineole to 3-carene is 1:0.1-10.

5. The repellent for adults of the spruce beetle as claimed in claim 1, characterized in that The volume ratio of 1,8-cineole to 3-carene is 1:

1.

6. Use of the repellent for adults of the spruce beetle as claimed in any one of claims 1 to 3 as a repellent for adults of the spruce beetle.

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