Trace element additive, feed and use thereof

Abstract

The invention discloses a trace element additive, feed and use. The trace element additive contains 19.7% to 41% of aminosugar iron, 15% to 35% of aminosugar zinc, 15% to 30% of aminosugar manganese, 6% to 15% of aminosugar copper, 0.05% to 0.30% of calcium iodate and 0.04% to 0.10% of sodium selenite in weight percentage; the aminosugar iron is methionine and Enteromorpha polysaccharide double chelated iron; the aminosugar zinc is methionine and Enteromorpha polysaccharide double chelated zinc; the aminosugar manganese is methionine and Enteromorpha polysaccharide double chelated manganese; the aminosugar copper is methionine and Enteromorpha polysaccharide double chelated copper. It is used to feed poultry and can improve the production energy and antioxidant properties of poultry.

Description

Technical Field

[0001] The present invention relates to the field of feed, and in particular to a trace element additive, feed and application thereof. Background Art

[0002] Trace elements are called the fifth type of nutrients that play an important role in the body. They have an extremely important impact on the current intensive and high-density animal production and reproduction. Iron, copper, manganese, zinc and selenium are the most critical essential trace elements in animal production. The five trace elements are usually used as cofactors or activators of various enzymes in animals, and play a very critical role in the growth, development, metabolism and immune regulation of livestock and poultry. At present, trace elements in animal breeding are divided into organic trace elements and inorganic trace elements. Inorganic trace elements are cheap and have a wide source of raw materials. They are most widely used in livestock and poultry production. At present, there are also many problems in the use of inorganic trace elements. They are easy to combine with anti-nutritional factors such as phytic acid in the intestine to form compounds that are difficult to digest and utilize; there is a certain antagonism between various inorganic trace elements, which affects the absorption and utilization rate of trace elements. In actual production, they are usually added in excess, which then causes stress and health problems for animals and serious environmental pollution problems. Organic trace elements are more common in single chelation of amino acid metal elements and polysaccharide metal elements. Organic trace elements are in a chelated state with a relatively stable chemical structure. There is no antagonistic effect in the intestine that affects the absorption of trace elements. In terms of bioavailability, organic trace elements can directly enter the body through the intestinal absorption channel, greatly improving the utilization rate of trace elements and reducing environmental pollution.

[0003] The improvement of animal immunity by polysaccharide trace element complexes is mainly reflected in its effect on lymphocyte proliferation; the absorption and utilization rate of amino acid chelated trace elements is higher than that of inorganic trace elements, and it can also reduce the environmental pollution problem of trace elements. Currently, the most common chelation forms in the market are single chelation, which refers to the process of combining only one organic and one inorganic substance in the same reaction system. Single chelation technology is relatively simple, but the chelation rate and chelation strength are low, and the high inorganic metal content is easy to pollute the environment. Summary of the invention

[0004] The purpose of the present invention is to replace the inorganic trace elements or amino acid single chelated organic trace elements, polysaccharide single chelated organic trace elements commonly used in poultry feed with methionine and Enteromorpha polysaccharide dual chelated organic trace elements, improve the production performance and antioxidant properties of poultry, improve the absorption and utilization rate of trace elements, and reduce environmental pollution problems.

[0005] To achieve the above object, the present invention provides a trace element additive, characterized in that it contains 19.7% to 41% by weight of aminosugar iron, 15% to 35% by weight of aminosugar zinc, 15% to 30% by weight of aminosugar manganese, 6% to 15% by weight of aminosugar copper, 0.05% to 0.30% by weight of calcium iodate, and 0.04% to 0.10% by weight of sodium selenite;

[0006] The aminosugar iron is a double chelated iron of methionine and Enteromorpha polysaccharide; its content can be 19.7%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 26.7%, 27%, 28%, 29%, 30%, 30.35%, 31%, 32%, 33%, 34%, 34.7%, 35%, 36%, 37%, 38%, 39%, 40%, 40.7%, 41%, but not limited to this.

[0007] Glucosamine zinc is a double chelated zinc of methionine and Enteromorpha polysaccharide; its content can be 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, but not limited to these.

[0008] Glucosamine manganese is a double chelated manganese of methionine and Enteromorpha polysaccharide; its content can be 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 22.5%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, but not limited thereto.

[0009] Copper glucosamine is double chelated copper of methionine and Enteromorpha polysaccharide, and its content can be 6%, 7%, 8%, 9%, 10%, 10.5%, 11%, 12%, 13%, 14%, 15%, but not limited thereto.

[0010] The calcium iodate content can be 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.10%, 0.11%, 0.12%, 0.13%, 0.14%, 0.15%, 0.16%, 0.17%, 0.18%, 0.19%, 0.20%, 0.21%, 0.22%, 0.23%, 0.24%, 0.25%, 0.26%, 0.27%, 0.28%, 0.29%, 0.30%, but is not limited to this.

[0011] The content of sodium selenite may be 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.10%, but is not limited thereto.

[0012] The invention also provides use of the trace element additive in raising poultry.

[0013] Furthermore, 0.04% to 0.1% of the trace element additive according to claim 1 is added to the basic diet; more preferably, 0.08% of the trace element additive is added to the basic diet.

[0014] Furthermore, the poultry is chicken or pig.

[0015] Furthermore, the use refers to the use of improving the production energy and antioxidant performance of poultry.

[0016] The present invention also provides a feed, characterized in that it contains the trace element additive; preferably, the weight content of the trace element additive is 0.04% to 0.1%; more preferably, the weight content of the trace element additive is 0.08%.

[0017] The invention adopts organic trace elements double-chelated by enteromorpha polysaccharide and methionine, which has the advantage of more fully improving the utilization efficiency of target trace elements and further reducing the pollution of trace element additives. DETAILED DESCRIPTION

[0018] Embodiments of the present invention are described in detail below, and the examples of the embodiments are intended to be used to explain the present invention, but should not be construed as limiting the present invention. In the embodiments, if specific techniques or conditions are not specified, they are carried out according to the techniques or conditions described in the literature in this area or according to the product specification. If the manufacturer is not specified for reagents or instruments used, they are all conventional products that can be obtained commercially.

[0019] The glucosamine iron of the present invention is double chelated iron of methionine and Enteromorpha polysaccharide, with an iron content of 28.57%; the glucosamine zinc is double chelated zinc of methionine and Enteromorpha polysaccharide, with a zinc content of 30%; the glucosamine manganese is double chelated manganese of methionine and Enteromorpha polysaccharide, with a manganese content of 30%; and the glucosamine copper is double chelated copper of methionine and Enteromorpha polysaccharide, with a copper content of 16.67%.

[0020] The preparation methods are:

[0021] Aminosugar iron: (1) For the first complexation reaction, add water to the reaction kettle, heat it, and start stirring to raise the temperature in the kettle to about 70°C. Then slowly add 1 mol of ferrous sulfate. After the addition is completed, continue to maintain 70°C and stir until the ferrous sulfate is completely dissolved.

[0022] Add 1 mol of L-lysine hydrochloride to the reactor evenly within 30 minutes for reaction, and control the temperature at about 70°C. After adding a certain amount, add 10% sodium carbonate solution to neutralize it and control the pH value to 5-6. After all the L-lysine hydrochloride is added, continue to maintain the temperature at 70°C and stir the reaction for 45 minutes. After that, add 10% sodium carbonate solution to neutralize it within 15 minutes to pH 6-7. After adding, measure the free L-lysine hydrochloride. If the free L-lysine hydrochloride content is less than 1%, the first complexation reaction ends. If the free L-lysine hydrochloride content is higher than 1%, continue to stir the reaction until it is less than 1% to reach the first complexation reaction end.

[0023] (2) Second complexation reaction After the first complexation reaction reaches the end point, the material is placed in the secondary reaction kettle, and 1 mol of Enteromorpha polysaccharide is evenly added within 30 minutes. After the addition, the temperature is maintained at about 60°C, and 10% sodium carbonate solution is added dropwise for neutralization, the pH value is controlled at 7-8, and the reaction is stirred for 45 minutes. The free iron ion content is measured. If the free iron ion content is less than 1%, the second complexation reaction ends.

[0024] (3) Granulation and drying: The materials after the reaction are placed in a mixer with ∑ blades, and 10 wt% of food-grade α-starch and 1 wt% of food-grade sodium carboxymethyl cellulose are added, and the mixture is uniformly mixed for 20 min to make the materials "clumped in the hand, and scattered by a pinch". After the materials are discharged, they are granulated by a swing granulator, pelletized by a shot blasting machine, dried in a boiling fluidized bed, cooled, sieved, and 20-60 mesh product particles are taken to obtain aminosugar iron.

[0025] Alternatively, (1) the first dry complex reaction: 1 mol of L-lysine hydrochloride, 0.2 mol of sodium carbonate and 20%wt of water are added to a mixing reactor equipped with ∑ blades, a heating jacket and a vacuum pumping function, and stirred at 70°C for 30 minutes. Then, 1 mol of ferrous sulfate is added, the machine cover is closed, and the mixing reactor is vacuumed to 1000 Pa, and the reaction is carried out under vacuum for 30 minutes. After the reaction is completed, the free L-lysine hydrochloride is sampled and determined. If the content of L-lysine hydrochloride is higher than 1%, the reaction is continued until it is lower than 1% to reach the end point of the first complex reaction.

[0026] (2) Second dry complexation reaction After the first complexation reaction reaches the end point, open the lid and add 1 mol Enteromorpha polysaccharide and 0.1 mol sodium carbonate. After the addition, cover the machine lid, evacuate the mixing reactor to 1000 Pa, maintain the temperature at 60 ° C, and continue the reaction for 30 minutes. At the end of the reaction, the material should be dry and loose, not wet and sticky. Sampling to determine the free iron ion content, if the free iron ion content is less than 1%, the second complexation reaction has reached the end point.

[0027] (3) The material after the dry granulation reaction is sent to a roller press through a feeder and pressurized to 30 MPa; the material is rolled into a dense block, cooled in a material bin with a cooling jacket at 2°C to 5°C, and then enters a crusher for crushing. After screening, 20-60 mesh product particles are taken to obtain aminosugar iron.

[0028] Glucosamine zinc: (1) For the first complex reaction, add water to the reaction kettle, heat it, and start stirring to raise the temperature in the kettle to about 70°C. Then slowly add 2 mol of zinc sulfate. After the addition is completed, continue to maintain 70°C and stir until the zinc sulfate is completely dissolved.

[0029] 1 mol of DL-methionine is added evenly to the reactor within 30 minutes for reaction. The temperature is controlled at about 70°C. After a certain amount is added, 10% sodium hydroxide solution is added dropwise to neutralize the reaction and the pH value is controlled at 5-6. After all the DL-methionine is added, the temperature is maintained at 70°C and the reaction is stirred for 45 minutes. After that, 10% sodium hydroxide solution is added dropwise within 15 minutes to neutralize the reaction to a pH of 6-7. After the addition is completed, the free DL-methionine is measured. If the free DL-methionine content is less than 1%, the first complexation reaction ends.

[0030] (2) Second complexation reaction After the first complexation reaction reaches the end point, the material is placed in the secondary reaction kettle, and 1 mol of brown algae oligosaccharide is evenly added within 30 minutes. After the addition, the temperature is maintained at about 60°C, and 10% sodium hydroxide solution is added dropwise for neutralization, the pH value is controlled at 7-8, and the reaction is stirred for 45 minutes. The free zinc ion content is measured. If the free zinc ion content is less than 1%, the second complexation reaction ends.

[0031] (3) Granulation and drying: The materials after the reaction are placed in a mixer with ∑ blades, and 10 wt% of food-grade α-starch and 2 wt% of food-grade sodium carboxymethyl cellulose are added, and the mixture is uniformly mixed for 20 minutes to make the materials "clumped in the hand, and scattered when squeezed". The materials are granulated by a swing granulator, pelletized by a shot blasting machine, dried in a boiling fluidized bed, cooled, sieved, and 20-60 mesh product particles are obtained to obtain glucosamine zinc.

[0032] Alternatively, (1) the first dry complex reaction is carried out by adding 1 mol of DL-methionine, 0.2 mol of sodium hydroxide and 30% wt of water to a mixing reactor equipped with a ∑ blade, a heating jacket and a vacuum pumping function, stirring and reacting at 65° C. for 35 minutes, then adding 2 mol of zinc sulfate, covering the machine cover, and vacuumizing the mixing reactor to 500 Pa, and reacting for 30 minutes under vacuum. After the reaction is completed, sampling is performed to determine the free DL-methionine. If the DL-methionine content is higher than 1%, the reaction is continued until it is lower than 1% to reach the end point of the first complex reaction.

[0033] (2) Second dry complexation reaction After the first complexation reaction reaches the end point, open the lid and add 1 mol of brown algae polysaccharide and 0.1 mol of sodium hydroxide. After the addition, cover the machine lid, evacuate the mixing reactor to 500 Pa, maintain the temperature at 65 ° C, and continue the reaction for 25 minutes. At the end of the reaction, the material should be dry and loose, not wet and sticky. Sampling to determine the free zinc ion content, if the free zinc ion content is less than 1%, the second complexation reaction has reached the end point.

[0034] (3) The material after the dry granulation reaction is sent to a roller press through a feeder and pressurized to 20 MPa; the material is rolled into a dense block, cooled in a material bin with a cooling jacket at 2°C to 5°C, and then enters a crusher for crushing. After screening, 20-60 mesh product particles are obtained to obtain glucosamine zinc.

[0035] Manganese Glucosamine: (1) For the first complexation reaction, add water to the reaction kettle, heat it, and start stirring to raise the temperature in the kettle to about 70°C. Then slowly add 1 mol of manganese sulfate. After the addition is completed, continue to maintain 70°C and stirring until all the manganese sulfate is dissolved.

[0036] 2 mol L-threonine is added evenly to the reactor within 30 minutes for reaction. The temperature is controlled at about 70°C. After a certain amount is added, 10% sodium carbonate solution is added dropwise to neutralize the reaction and the pH value is controlled at 5-6. After all the L-threonine is added, the temperature is maintained at 70°C and the reaction is stirred for 45 minutes. After that, 10% sodium carbonate solution is added dropwise within 15 minutes to neutralize the reaction to a pH of 6-7. After the addition is completed, the free L-threonine is measured. If the free L-threonine content is less than 1%, the first complexation reaction ends.

[0037] (2) Second complexation reaction After the first complexation reaction reaches the end point, the material is placed in the second reaction kettle, and 1 mol of Ulva polysaccharide is added evenly within 30 minutes. After the addition, the temperature is maintained at about 60°C, and 10% sodium carbonate solution is added dropwise for neutralization, the pH value is controlled at 7-8, and the reaction is stirred for 45 minutes. The free manganese ion content is measured. If the free manganese ion content is less than 1%, the second complexation reaction ends.

[0038] (3) Granulation and drying: The materials after the reaction are placed in a mixer with ∑ blades, and 12 wt% of food-grade α-starch and 1 wt% of food-grade sodium carboxymethyl cellulose are added, and the mixture is uniformly mixed for 20 minutes to make the materials "clumped in the hand, and scattered when squeezed". The materials are granulated by a swing granulator, pelletized by a shot blasting machine, dried in a boiling fluidized bed, cooled, sieved, and 20-60 mesh product particles are obtained to obtain aminoglucose manganese.

[0039] Alternatively, (1) the first dry complex reaction is to add 2 mol of L-threonine, 0.2 mol of sodium carbonate and 30% wt of water in a mixing reactor equipped with ∑ blades, a heating jacket and a vacuum pumping function, and stir the reaction at 75° C. for 25 minutes, then add 1 mol of manganese sulfate, cover the machine, and evacuate the mixing reactor to 1000 Pa, and react for 30 minutes under vacuum. After the reaction is completed, sample and measure the free L-threonine. If the L-threonine content is higher than 1%, continue the reaction until it is lower than 1% to reach the end point of the first complex reaction.

[0040] (2) Second dry complexation reaction After the first complexation reaction reaches the end point, open the lid and add 1 mol of Ulva polysaccharide and 0.2 mol of sodium carbonate. After the addition, cover the machine lid, evacuate the mixing reactor to 1000 Pa, maintain the temperature at 55 ° C, and continue the reaction for 35 minutes. At the end of the reaction, the material should be dry and loose, not wet and sticky. Sampling to determine the free manganese ion content, if the free manganese ion content is less than 1%, the second complexation reaction has reached the end point.

[0041] (3) The material after the dry granulation reaction is sent to a roller press through a feeder and pressurized to 20 MPa; the material is rolled into a dense block, cooled in a material bin with a cooling jacket at 2°C to 5°C, and then enters a crusher for crushing. After screening, 20-60 mesh product particles are taken to obtain aminoglucosamine manganese.

[0042] Copper Glucosamine: (1) For the first complex reaction, add water to the reaction kettle, heat it, and start stirring to raise the temperature in the kettle to about 70°C. Then slowly add 2 mol of copper sulfate. After the addition is completed, continue to maintain 70°C and stir until the copper sulfate is completely dissolved.

[0043] 2 mol of DL-methionine is added evenly to the reactor within 30 minutes for reaction. The temperature is controlled at about 70°C. After a certain amount is added, 10% sodium hydroxide solution is added dropwise to neutralize the reaction and the pH value is controlled at 5-6. After all the DL-methionine is added, the temperature is maintained at 70°C and the reaction is stirred for 45 minutes. After that, 10% sodium hydroxide solution is added dropwise within 15 minutes to neutralize the reaction to a pH of 6-7. After the addition is completed, the free DL-methionine is measured. If the free DL-methionine content is less than 1%, the first complexation reaction ends.

[0044] (2) Second complexation reaction After the first complexation reaction reaches the end point, the material is placed in the secondary reaction kettle, and 1 mol of brown algae polysaccharide is evenly added within 30 minutes. After the addition, the temperature is maintained at about 60°C, and 10% sodium hydroxide solution is added dropwise for neutralization, the pH value is controlled at 7-8, and the reaction is stirred for 45 minutes. The free copper ion content is measured. If the free copper ion content is less than 1%, the second complexation reaction has reached the end point.

[0045] (3) Granulation and drying: The materials after the reaction are placed in a mixer with ∑ blades, and 12 wt% of food-grade α-starch and 2 wt% of food-grade sodium carboxymethyl cellulose are added, and the mixture is uniformly mixed for 20 minutes to make the materials "clumped in the hand and scattered by a pinch". The materials are granulated by a swing granulator, pelletized by a shot blasting machine, dried in a boiling fluidized bed, cooled, sieved, and 20-60 mesh product particles are obtained to obtain aminoglucose copper.

[0046] Alternatively, (1) the first dry complex reaction is to add 2 mol of DL-methionine, 0.1 mol of sodium hydroxide and 25% wt of water to a mixing reactor equipped with ∑ blades, a heating jacket and a vacuum pumping function, and stir the reaction at 70° C. for 25 minutes, then add 2 mol of copper sulfate, cover the machine, and vacuumize the mixing reactor to 800 Pa, and react for 30 minutes under vacuum. After the reaction is completed, sample and measure the free DL-methionine. If the DL-methionine content is higher than 1%, continue the reaction until it is lower than 1% to reach the end point of the first complex reaction.

[0047] (2) Second dry complexation reaction After the first complexation reaction reaches the end point, open the lid and add 1 mol of brown algae polysaccharide and 0.1 mol of sodium hydroxide. After the addition, cover the machine lid, evacuate the mixing reactor to 800 Pa, maintain the temperature at 65 ° C, and continue the reaction for 30 minutes. At the end of the reaction, the material should be dry and loose, not wet and sticky. Sampling to determine the free copper ion content, if the free copper ion content is less than 1%, the second complexation reaction has reached the end point.

[0048] (3) The material after the dry granulation reaction is sent to a roller press through a feeder and pressurized to 25 MPa; the material is rolled into a dense block, cooled in a material bin with a cooling jacket at 2°C to 5°C, and then enters a crusher for crushing. After screening, 20-60 mesh product particles are taken to obtain aminoglucose copper.

[0049] Example 1: Preparation of trace element additives

[0050] Raw materials: see Table 1.

[0051] Preparation method: Mix the above raw materials.

[0052] Example 2: Preparation of trace element additives

[0053] Raw materials: see Table 1.

[0054] Preparation method: Same as Example 1.

[0055] Example 3: Preparation of trace element additives

[0056] Raw materials: see Table 1.

[0057] Preparation method: Same as Example 1.

[0058] Example 4: Preparation of trace element additives

[0059] Raw materials: see Table 1.

[0060] Preparation method: Same as Example 1.

[0061] Example 5: Preparation of trace element additives

[0062] Raw materials: see Table 1.

[0063] Preparation method: Same as Example 1.

[0064] Example 6: Preparation of trace element additives

[0065] Raw materials: see Table 1.

[0066] Preparation method: Same as Example 1.

[0067] Blank comparison: Preparation of trace element additives

[0068] Raw materials: see Table 1.

[0069] Preparation method: Same as Example 1.

[0070] Comparative Example 1: Preparation of trace element additives

[0071] Raw materials: see Table 1.

[0072] Preparation method: Same as Example 1.

[0073] Comparative Example 2: Preparation of trace element additives

[0074] Raw materials: see Table 1.

[0075] Preparation method: Same as Example 1.

[0076] Table 1 Proportions of each component of the trace element additives of Examples 1-6 and Control Experiments (wt%)

[0077]

[0078] Note: Methionine iron, methionine zinc, methionine manganese, and methionine copper were purchased from Chongqing Senlemei Biotechnology Co., Ltd.; Astragalus polysaccharide was purchased from Shifang Hengruikang Biotechnology Co., Ltd.

[0079] Example 5: Effect verification experiment

[0080] 1. Feeding Chickens

[0081] The additives of each group were prepared into trace element additives according to the raw material formula in Table 1. Then, 0.1% by mass was added to the basic diet of laying hens for feeding.

[0082] The experimental basal diet was prepared based on NY / T 33-2004 and NRC (1994) in combination with the Hy-Line Brown laying hen feeding manual. The test used a corn-soybean meal (powder) type diet (Table 2).

[0083] 384 30-week-old Hy-Line brown laying hens with similar production performance were raised in a semi-open chicken house with three-layer three-dimensional cages, with 5 cages (50cm×50cm×45cm) connected in each row, each cage as a replicate, and 12 replicates per experimental group, with 4 chickens in each cage (96 cages in total), and each replicate was evenly distributed on the upper, middle and lower three rows of stepped cages. Natural and artificial lighting were used, with constant light for 16 hours a day (automatic lighting control system) and an intensity of 15LX.

[0084] Feed dry powder, free to eat, feed once at 07:00 and 15:00 every day, mix feed 4 times; nipple waterer, free drinking water. According to normal procedures for epidemic prevention, special management; disinfection of chickens and cleaning of chicken manure once a week, routine immunization; record temperature, humidity and weather conditions at 14:30 every day; change with climate, season and sunlight, the temperature range during the test is 4.0-15.0℃; relative humidity is 40%-70%. The test lasts for 8 weeks, and relevant production data are counted and recorded. The eggshell strength was measured using an FGX.5R eggshell strength tester. The egg was placed vertically on the eggshell strength tester with the blunt end facing upward, and the pressure per unit area of ​​the eggshell surface was measured in kg / cm2; eggshell thickness: measured using an ETG-1061A eggshell thickness tester. The thickness values ​​at the blunt end, middle end, and sharp end were measured respectively, and the average value was calculated in mm; egg shape index: the longitudinal and transverse diameters of the egg were measured using a vernier caliper in mm with an accuracy of 0.1 mm, and the egg shape index = longitudinal diameter / transverse diameter; eggshell color: measured using a QcR eggshell color tester, with white being 100 and black being 0.

[0085] Table 2: Raw material formula of basic diet for laying fowl

[0086]

[0087]

[0088] Table 3 Trace element addition levels in feed and measured values

[0089]

[0090] Table 4 Effects of adding different trace elements to the diet on the production performance of laying hens

[0091]

[0092]

[0093] SPSS statistics 17 was used for difference analysis. The same letters or no letters for the same item indicated no significant difference (P>0.05), and different letters indicated significant difference (P<0.05).

[0094] From the data in Table 4, we can see that:

[0095] 1) In terms of egg production rate, Examples 1 to 6 were higher than the blank control group and Comparative Examples 1 to 2, wherein the egg production rate of Example 4 was the highest at 94.31%, and the egg production rate of the blank control was the lowest at 91.07%. There were significant differences between Examples 1 to 6 and the blank control and Comparative Examples 1 to 2.

[0096] 2) In terms of average egg weight, there was no significant difference among the test groups, and the egg weight of Example 4 was relatively heavy at 60.84 g.

[0097] 3) In terms of the rate of broken eggs, Example 4 was the lowest at 2.37%, the blank control was the highest at 3.58%, and Comparative Examples 1 to 2 were 2.94% and 2.78%, respectively, which were lower than the blank control group, but higher than Examples 1 to 6. There were significant differences between Examples 1 to 6 and the blank control and Comparative Example 2.

[0098] 4) In terms of eggshell strength, the strength of Examples 1 to 6 is higher than that of the blank control and Comparative Examples 1 to 2, and the eggshell strength of Example 3 is the highest at 3.07 kg / cm 2 The eggshell strength of comparative examples 1-2 is also better than that of the blank control, but worse than that of embodiments 1 to 6. There are significant differences between embodiments 1 to 5 and the blank control and comparative examples 1-2.

[0099] 5) In terms of eggshell thickness, the eggshell thicknesses of Examples 1 to 6 are 0.31 mm, 0.33 mm, 0.34 mm, 0.36 mm, 0.32 mm, and 0.29 mm, respectively, while the blank control is 0.24 mm, and the comparative examples 1-2 are 0.30 mm and 0.29 mm, respectively. Among them, the eggshell of Example 3 is the thickest, and the blank control eggshell is the thinnest. There are significant differences between Examples 1 to 5 and the blank control and comparative example 2.

[0100] 6) In terms of egg shape index, there was no significant difference among the groups, but the egg shapes of Examples 1 to 6 were more oval, while the egg shape of the blank control was elongated.

[0101] 7) In terms of eggshell color, the colors of Examples 1 to 6 are higher than those of the blank control and Comparative Examples 1 to 2, and there are significant differences between Examples 1 to 4 and the blank control and Comparative Example 2.

[0102] Antioxidant index of laying hen serum:

[0103] One chicken was randomly selected for each replicate, and 5 mL of blood was collected from the wing vein. The blood was allowed to stand for 30 min, centrifuged at 3000 r / min for 15 min, and the upper serum was taken and placed in a centrifuge tube and stored at -20°C for later use. The total antioxidant capacity (T-AOC), lipid oxidation (MDA), and total superoxide dismutase (T-SOD) were determined using an ELISA instrument. The results are shown in Table 5.

[0104] Table 5 Effects of trace element supplementation on serum antioxidant indexes of laying hens

[0105]

[0106]

[0107] SPSS statistics 17 was used for difference analysis. The same letters or no letters for the same item indicated no significant difference (P>0.05), and different letters indicated significant difference (P<0.05).

[0108] It can be seen from the above table that in the T-AOC index: Examples 1 to 6 are higher than the blank control and comparative examples 1 to 2, comparative example 2 is slightly lower than each example, and there are significant differences between Examples 1 to 6 and the blank control group and comparative example 1. In the MDA index: the blank control is the highest, comparative example 1 is second, comparative example 2 is lower than the blank control and comparative example 1 but higher than Examples 1 to 6, and there are significant differences between Examples 1 to 6 and the blank control group and comparative example 1 to 2. In the GSH-Px index: the blank control is the highest, comparative example 2 is lower than the blank control and comparative example 1 but higher than Examples 1 to 6, and there are significant differences between Examples 1 to 6 and the blank control group and comparative example 1; in the T-SOD index: Example 5 is the highest at 161.65U / mL, the blank control is the lowest, and there are significant differences between Examples 1 to 6 and the blank control group and comparative example 1. Trace element content in laying hen manure:

[0109] The whole feces method was used to collect laying hen manure. Before collecting manure, the chickens were stopped from feeding for 12 hours, and then they were fed freely for three days. The feces were collected for three days, and then they were stopped from feeding for 12 hours. During the experiment, the chickens were fed with pellets and had free access to water. The collected feces were placed in a sample bag and stored in a refrigerator at 20°C for later use. The feed intake, feces excretion and sampling volume were accurately recorded. The samples were collected by the quartering method, dried in an oven at 65°C to constant weight, crushed through 40 mesh, and tested. The contents of copper, iron, zinc and manganese in the feces were detected by atomic absorption spectrophotometer. The detection method refers to the "Determination of Calcium, Copper, Iron, Magnesium, Manganese, Potassium, Sodium and Zinc in Feed by Atomic Absorption Spectrometry" (GB / T 13885-2017).

[0110] Table 6 Effects of adding different trace elements to the diet on the trace element content in laying hen feces

[0111]

[0112]

[0113] SPSS statistics 17 was used for difference analysis. The same letters or no letters for the same item indicated no significant difference (P>0.05), and different letters indicated significant difference (P<0.05).

[0114] As can be seen from Table 6, when the trace element content in feces of each test group was detected, it was found that the trace element content in feces of Examples 1 to 6 was the lowest, and the trace element content in feces of the blank control was the highest. Comparative Example 1 added an organic trace element product, and its trace element content in feces was lower than that of the blank control, but higher than that of the embodiments.

[0115] 2. Feeding Pigs:

[0116] Experimental subjects: A total of 60 28-day-old weaned piglets of Duroc×Changchun×Dalian hybrid breed, which were in good condition and had an average body weight of (7.28±0.66) kg and were raised in the experimental field, were selected as experimental subjects.

[0117] Experimental design and grouping: 60 experimental pigs were randomly divided into 3 groups, including the basic diet group (control group), experimental group 1, experimental group 2, and experimental group 3. The control group added ordinary inorganic trace elements to the basic diet; experimental group 1 added glucosamine trace elements to the basic diet; experimental group 2 added methionine chelated organic trace elements to the basic diet; experimental group 3 added methionine astragalus polysaccharide chelated trace elements to the basic diet. Each treatment group had 10 replicates, with 2 pigs in each replicate. The experimental period was 35 days, with the first week as the pre-test period and the formal test period of 28 days.

[0118] Effects on the production performance of weaned piglets:

[0119] At 8:00 on the day when the experiment started and ended, each piglet was weighed on an empty stomach (fasted for 12 hours) to calculate the average daily weight gain; at the end of the experiment, the piglet feed intake was counted, the average daily feed intake of each repeated piglet was calculated, and the feed-to-weight ratio was calculated. The results are shown in Table 7.

[0120] Table 7 Effects of adding different trace elements on the growth performance of weaned piglets

[0121]

[0122] SPSS statistics 17 was used for difference analysis. The same letters or no letters for the same item indicated no significant difference (P>0.05), and different letters indicated significant difference (P<0.05).

[0123] As shown in Table 7, there was no significant difference in initial body weight between the control group and the experimental group, indicating that the experimental grouping was uniform. In terms of final body weight, the lowest in the control group was 14.71 kg, and the heaviest in the experimental group 1 was 17.01 kg. There were significant differences between the experimental group 1 and the control group and the experimental group 2, and there were no significant differences between the other groups. In terms of average feed intake, the highest feed intake in the experimental group 3 was 509.94 g / t, and the feed intake in the experimental group 1 was 509.78 g / d, which was basically the same. The lowest feed intake in the control group was 475.12 g / d, and there was no significant difference among the groups; in terms of average daily weight gain, the highest was 346.79g / d in the test group 1, and the lowest was 265.43g / d in the control group. The average daily weight gain of the test groups 2 and 3 was higher than that of the control group. There was a significant difference between the test group 1 and the control group and the test group 2, but no significant difference between the test group 1 and the test group 3; in terms of feed-to-weight ratio, the feed-to-weight ratio of the test groups was lower than that of the control group. The feed-to-weight ratio of the test group 1 was 1.47, and the feed-to-weight ratio of the control group was the highest at 1.79. There was a significant difference between the test group 1 and the other groups.

[0124] Effects on trace elements in feces of weaned piglets:

[0125] On the day of the end, the feces were dried at 65°C, crushed through 40 meshes, and tested. The content of copper, iron, zinc, and manganese in the feces was detected by atomic absorption spectrophotometer. The detection method was based on the determination of calcium, copper, iron, magnesium, manganese, potassium, sodium, and zinc in feed by atomic absorption spectrometry (GB / T 13885-2017). The results are shown in Table 8.

[0126] Table 8 Effects of adding different trace elements on the content of trace elements in raw feces of weaned piglets

[0127]

[0128] SPSS statistics 17 was used for difference analysis. The same letters or no letters for the same item indicated no significant difference (P>0.05), and different letters indicated significant difference (P<0.05).

[0129] It can be seen from Table 8 that, compared with the control group, the experimental groups with added organic trace elements had lower trace element contents in feces than the control group. The copper, iron, manganese and zinc contents of the experimental groups were significantly different from those of the control group, indicating that organic trace elements can be better absorbed and utilized by weaned piglets than inorganic trace elements. Among the experimental groups, the contents of copper, iron, manganese and zinc in feces of Experimental Group 1 were 104.39 mg / kg, 1496.43 mg / kg, 248.95 mg / kg and 755.47 mg / kg, respectively, which were lower than those of Experimental Groups 2 and 3, indicating that Experimental Group 1 was more able to make full use of the added trace elements.

[0130] Effects on antioxidant indicators of weaned piglets:

[0131] At the end of the experiment, one weaned piglet with a weight close to the average was selected for each replicate. 10 mL of blood was collected from the anterior vena cava on an empty stomach with a 10 mL disposable syringe and quickly transferred to a 10 mL centrifuge tube. After being tilted and allowed to stand for 1 hour at room temperature, the tube was centrifuged at 3000 r / min for 15 minutes to separate the serum and store at -20°C for testing. The determination of catalase (CAT), total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC) and other tests were performed according to the instructions and measured using a visible spectrophotometer. The results are shown in Table 9.

[0132] Table 9 Effects of adding different trace elements on the antioxidant indexes in the serum of weaned piglets

[0133]

[0134] SPSS statistics 17 was used for difference analysis. The same letters or no letters for the same item indicated no significant difference (P>0.05), and different letters indicated significant difference (P<0.05).

[0135] From the data in Table 9, it can be seen that the T-AOC index: the control group and test group 2 are lower, the highest in test group 1 containing polysaccharides is 2.89U / mL, and the test group 3 also containing polysaccharides is 2.13U / mL. There are significant differences between each test group, and there are no significant differences between each test group. However, the T-AOC value in the serum of test group 1 is higher than that of test groups 2 and 3. CAT index: the highest in the control group is 14.09U / mL, the test group 2 is 11.49U / mL, the test group 3 is slightly higher than the test group 1, and the lowest in test group 1 is 8.16U / mL, and there is a significant difference between the test groups and the control group. T-SOD index: the highest in test group 1 is 68.29U / mL, the test group 3 is 54.55U / mL, and the lowest in the control group is 34.38U / mL, and there are significant differences between each test group and the control group. MDA index: the highest in the control group is 2.52nmol / mL, and the lowest in test group 1 is 1.21nmol / mL, and there are significant differences between the test groups and the control group, and there are no significant differences between the test groups.

[0136] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention without departing from the principles and intent of the present invention.

Claims

1. A trace element additive, characterized in that: Containing by weight percentage 19.7% to 41% of aminosugar iron, 15% to 35% of aminosugar zinc, 15% to 30% of aminosugar manganese, 6% to 15% of aminosugar copper, 0.05% to 0.30% of calcium iodate, and 0.04% to 0.10% of sodium selenite; The aminoglucose iron is methionine and Enteromorpha polysaccharide double chelated iron; the aminoglucose zinc is methionine and Enteromorpha polysaccharide double chelated zinc; the aminoglucose manganese is methionine and Enteromorpha polysaccharide double chelated manganese; the aminoglucose copper is methionine and Enteromorpha polysaccharide double chelated copper; The trace element additives are added to the basic diet.

2. The trace element additive according to claim 1, characterized in that: The invention contains 34.7% of aminosugar iron, 29% of aminosugar zinc, 26% of aminosugar manganese, 10% of aminosugar copper, 0.2% of calcium iodate and 0.1% of sodium selenite in weight percentage.

3. Use of the trace element additive according to claim 1 in raising poultry.

4. The use according to claim 3, characterized in that Add 0.04% to 0.1% of the trace element additive according to claim 1 to the basic diet.

5. The use according to claim 4, characterized in that Add 0.08% of the trace element additive according to claim 1 to the basic diet.

6. The use according to claim 3, characterized in that: The poultry is chicken.

7. Use of the trace element additive according to claim 1 in pig breeding.

8. The use according to claim 7, characterized in that The use is to improve the production performance and antioxidant performance of pigs.

9. The use according to claim 3, characterized in that: The use is to improve the production performance and antioxidant performance of poultry.

10. A feed, characterized in that: Contains the trace element additive according to any one of claims 1-2.

11. The feed according to claim 10, characterized in that The weight content of the trace element additive is 0.04% to 0.1%.

12. The feed according to claim 10, characterized in that The weight content of the trace element additive is 0.08%.

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