Plant growth regulator for promoting root growth and increasing yield and its application

Abstract

The invention discloses a plant growth regulator for promoting root growth and increasing yield and its application, which relates to the technical field of agricultural fertilizers, including a growth regulator in which the active ingredients are active ingredient A, active ingredient B and active ingredient C, and the mass ratio of active ingredient A: active ingredient B: active ingredient C is 1-100: 10-1000: 100-10000; active ingredient A is persimmon tannin, active ingredient B is naphthylacetic acid, and active ingredient C is sodium nitrophenolate. The invention has a significant synergistic effect in regulating or promoting plant growth, can promote the development of crop roots and increase yield, improve crop quality, and improve economic and social benefits, and can effectively solve the problem that the existing compound preparations cannot have multiple effects on crops and have weak yield-increasing and synergistic effects.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural fertilizers, and in particular to a plant growth regulator for promoting root growth and increasing yield and its application. Background Art

[0002] At present, naphthylacetic acid and sodium nitrophenolate are widely used in the fields of pesticides and fertilizers as active ingredients of plant growth regulators to prepare different dosage forms, and the two can be used together as active ingredients of many types of preparations (such as insecticides, seed dressings and fertilizers, etc.); further, in the field of pesticides, a seed dressing agent for potatoes containing sodium nitrophenolate for insect and disease prevention has been developed, and a seed soaking agent formula containing naphthylacetic acid and sodium nitrophenolate for improving germination rate has been developed. In the field of fertilizers, a variety of synergistic fertilizer formulas such as water-soluble fertilizers, foliar fertilizers, and flushing fertilizers containing naphthylacetic acid and sodium nitrophenolate have been developed. Naphthylacetic acid and sodium nitrophenolate are used as plant growth regulators. Nutrient solutions that can promote root absorption and increase seedling rate and treatment agents that improve bud grafting survival rate have been developed for fruit trees, root-promoting and seedling-strengthening agents have been developed for tobacco, preparations that improve fruit setting rate have been developed for tomatoes, and seedling regulators have been developed for vegetables.

[0003] With the development of agricultural technology, the application of plant growth regulators on crops has gradually been valued. According to the different working principles of plant growth regulators, reasonable compounding can better improve the effect than using a single one. In order to help farmers increase production and income, it is of great significance to develop a formula of plant growth regulators that promotes crops and use them in agricultural production.

[0004] However, most of the existing compound preparations only have a single effect on crops, or although they have a promoting effect on crops, the promoting effect cannot meet the requirements of increasing production and increasing efficiency. Some compound preparations even have antagonistic effects during application, and the effects are not as good as using a single agent.

[0005] In summary, existing compound preparations have the problems of being unable to target multiple effects on crops and having weak yield-increasing and efficiency-enhancing effects. Summary of the invention

[0006] The purpose of the present invention is to provide a plant growth regulator for promoting root growth and increasing yield and its application, so as to solve the problem that the existing compound preparations in the above-mentioned background technology cannot have multiple effects on crops and have weak yield-increasing and synergistic effects.

[0007] To achieve the above object, the present invention provides a plant growth regulator for promoting root growth and increasing yield, comprising active ingredients of the growth regulator, namely active ingredient A, active ingredient B and active ingredient C, wherein the mass ratio of active ingredient A: active ingredient B: active ingredient C is 1-100: 10-1000: 100-10000.

[0008] Furthermore, the active ingredient A is persimmon tannin, the active ingredient B is naphthylacetic acid, and the active ingredient C is sodium nitrophenolate.

[0009] Furthermore, the mass ratio of active ingredient A:active ingredient B:active ingredient C is 1:10:100.

[0010] Furthermore, the concentration range of active ingredient A is 0.01-0.1 mg / L, the concentration range of active ingredient B is 0.1-1 mg / L, and the concentration range of active ingredient C is 1-10 mg / L.

[0011] Furthermore, the concentration of active ingredient A is 0.1 mg / L, the concentration of active ingredient B is 1 mg / L, and the concentration of active ingredient C is 10 mg / L.

[0012] Furthermore, the dosage form of the plant growth regulator is one of an aqueous solution, a wettable powder, a water-dispersible granule, an oil suspension, a suspoemulsion, an emulsifiable concentrate, a microemulsion, an emulsion in water, a soluble granule or a soluble powder.

[0013] A method for preparing a plant growth regulator for promoting root growth and increasing production, wherein the method for preparing the active ingredient A persimmon tannin comprises the following steps:

[0014] 1) Raw material processing: washing and crushing the persimmons;

[0015] 2) Ultrahigh pressure treatment: adding purified water to the crushed persimmons in step 1), and then adjusting the pressure and the action temperature to perform ultrahigh pressure treatment;

[0016] 3) Ultrasonic treatment: subjecting the solution obtained in step 2) to ultrasonic treatment;

[0017] 4) Enzymatic hydrolysis: placing the solution obtained in step 3) in a sealed fermentation tank, adding activated complex enzymes of pectinase, apple amylase and pectin lyase for enzymatic hydrolysis;

[0018] 5) filtering and concentrating the enzymatic hydrolyzate obtained in step 4), and spray-drying the solution to obtain persimmon tannin.

[0019] Furthermore, in step 2), 5-10 times the weight of pure water is added, the pressure is 350 MPa, the action temperature is 40-50° C., and the ultra-high pressure treatment time is 2-5 min.

[0020] Furthermore, in step 3), the ultrasonic treatment time is 5-10 min, the action temperature is 40-50° C., and the power is 300 W.

[0021] Furthermore, in step 4), the enzymolysis time is 20-30 minutes, the enzymolysis temperature is 43-45° C., the weight ratio of pectinase, apple amylase and pectin lyase is 5:3:2, and the amount of complex enzyme added is 40-80 mg / kg.

[0022] The invention discloses an application of a plant growth regulator for promoting root growth and increasing yield in regulating crop growth.

[0023] Furthermore, the crops include leafy vegetable crops and cereal crops.

[0024] Furthermore, the specific steps of the application are:

[0025] S1: Select crops;

[0026] S2: preparing ternary formulation ingredients;

[0027] S3: Mix with base fertilizer for fertilization. The ternary formula components in S2 are fixed to volume with water, then mixed with base fertilizer and applied to the crop soil in S1;

[0028] S4: After fertilizing in S3, wait for the crops to mature.

[0029] Furthermore, the ternary formula preparation ingredients in S2 include: active ingredient A at a concentration of 0.1 mg / L, active ingredient B at a concentration of 1 mg / L, and active ingredient C at a concentration of 10 mg / L.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] 1. The persimmon tannin, naphthylacetic acid and sodium nitrophenolate in the growth regulator composition of the present invention are compounded in a ratio of 1-100:10-1000:100-10000, which promotes the growth and development of crop roots, increases crop yields, improves crop stress resistance and improves crop quality, and will not remain in the plant body;

[0032] 2. The frequency and amount of use of the plant growth regulator of the present invention vary slightly with the type of crop, growth environment, and weather and climate. The purpose of promoting root growth and increasing yield can be achieved by using a suitable dosage or a suitable component ratio;

[0033] 3. The components of the formula of the present invention have good solubility, are easy to be applied in agricultural production, and have a wide range of drug concentrations. High concentrations are not likely to cause drug damage to crops, and the drug effect can still be guaranteed at low concentrations;

[0034] 4. The formulation ingredients of the preparation of the present invention can be directly added during the granulation of fertilizer production and sown together with the fertilizer in the form of base fertilizer. In actual production, the manpower, material resources and costs of secondary application of pesticides are reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a comparison chart of the root growth of Zhengdan 958 corn with different formulas;

[0036] Figure 2This is a comparison chart of the yields of different formulas of Zhongdefeng No. 6 corn. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] The plant growth regulator for promoting root growth and increasing yield in this embodiment, the active ingredients of the growth regulator are active ingredient A, active ingredient B and active ingredient C, the mass ratio of active ingredient A: active ingredient B: active ingredient C is 1:10:100, the concentration of active ingredient A is 0.1 mg / L, the concentration of active ingredient B is 1 mg / L, and the concentration of active ingredient C is 10 mg / L.

[0039] In this solution, the active ingredient A is persimmon tannin, the active ingredient B is naphthylacetic acid, and the active ingredient C is sodium nitrophenolate;

[0040] The preparation method of the active ingredient A persimmon tannin is as follows:

[0041] 1) Raw material processing: Wash and crush the persimmons that are 70% to 80% ripe;

[0042] 2) Ultrahigh pressure treatment: add 5-10 times the weight of pure water to the crushed persimmons in step 1), with a pressure of 350 MPa and an action temperature of 40-50° C., and perform ultrahigh pressure treatment for 2-5 minutes;

[0043] 3) Ultrasonic treatment: The solution obtained in step 2) is subjected to ultrasonic treatment for 5-10 min at a temperature of 40-50° C. and a power of 300 W;

[0044] 4) Enzymolysis: placing the solution obtained in step 3) in a sealed fermentation tank, adding activated complex enzymes of pectinase, apple amylase and pectin lyase for enzymolysis, the enzymolysis time is 20-30 minutes, the enzymolysis temperature is 43-45° C., the weight ratio of pectinase, apple amylase and pectin lyase is 5:3:2, and the amount of complex enzyme added is 40-80 mg / kg;

[0045] 5) filtering and concentrating the enzymatic hydrolyzate obtained in step 4), and spray-drying the solution to obtain persimmon tannin.

[0046] Naphthylacetic acid and sodium nitrophenolate are prepared according to the existing preparation method; wherein, the auxiliary agent is selected according to the conventional method;

[0047] The filler auxiliary agent can be selected from one or more of a solvent, a dispersant, a wetting agent, an adhesive, a thickener, a film former, a binder and a surfactant.

[0048] The solvent is one or more of water, benzene, xylene, methanol, ethanol, n-butanol, ethyl acetate, n-hexane, N-methylpyrrolidone, dimethylformamide and dimethyl sulfoxide.

[0049] The stabilizer is one or more of citrate, citric acid, hydrochloric acid, sulfuric acid, phosphoric acid and potassium dihydrogen phosphate.

[0050] The surfactant is one or more of fatty alcohol polyoxyethylene ether, sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, polyethylene glycol, alkylphenol polyoxyethylene ether, and alkylphenol polyoxyethylene polyoxypropylene ether block polymer.

[0051] Furthermore, the dosage form of the plant growth regulator of the present invention is one of aqueous solution, wettable powder, water dispersible granules, oil suspension, suspoemulsion, emulsifiable concentrate, microemulsion, emulsion in water, soluble granules or soluble powder.

[0052] The invention discloses an application of a plant growth regulator for promoting root growth and increasing yield in regulating crop growth.

[0053] Crops include leafy vegetables and cereal crops.

[0054] In this scenario, the crops are Chinese cabbage and corn, as shown below:

[0055] Example 1: Pakchoy Indoor Potted Planting Drug Efficacy Test

[0056] (1) Preparation ingredients:

[0057] According to the existing conventional preparation methods, each preparation uses the above-mentioned composition as the active ingredient, the active ingredient A is persimmon tannin, the active ingredient B is naphthylacetic acid, and the active ingredient C is sodium nitrophenolate. Now take the aqueous preparation as an example, the preparation ingredients are detailed in Table 1; at the same time, the corresponding control preparations are prepared, as shown in Table 2, and the above-mentioned preparations are all diluted to the corresponding concentrations with water.

[0058] Table 1: Ingredients of three preparations of the present invention

[0059] Preparation ingredients Recipe A Recipe B Recipe C Active ingredient A (mg / L) 0.8 0.1 0.05 Active ingredient B (mg / L) 8 1 0.5 Active ingredient C (mg / L) 80 10 5

[0060] Table 2: Comparative formulation ingredients

[0061]

[0062] (2) Test object: Brassica chinensis

[0063] Test method: The test was conducted in the glass greenhouse of Shenyang Institute of Chemical Industry. Peat soil (purchased from Nanhu Flower Market in Shenyang) and sand were mixed in a 1:1 ratio and evenly loaded into a 54cm*27cm*6cm seedling tray. The seeds were evenly sown in the seedling tray and covered with a soil layer about 0.5cm thick. Watering was carried out from the bottom, and the seeds were germinated and seedled at 24-28℃. After the pakchoy plants grew to 2 true leaves, the pakchoy seedlings with consistent growth were selected and transplanted into pots. The growth conditions of each pot of seedlings were kept consistent. After the seedlings were slow to grow, base fertilizer and the above-mentioned preparations and control preparations were applied. The base fertilizer is compound fertilizer 15-15-15, and the dosage is 300kg / hm 2 The dosage of the preparation was 20 g of active ingredient added to 1 t of fertilizer. The relevant active ingredients were fixed to volume with water according to the active ingredient ratio and concentration in Table 1 and Table 2, mixed with fertilizer and applied to the soil. The root growth of Chinese cabbage was investigated after 20 days. Each treatment was repeated 5 times. The test results are shown in Table 3.

[0064] Table 3: Data from the potted plant experiment of Chinese cabbage

[0065]

[0066] As shown in Table 3, the results of the potted plant test show that persimmon tannin, naphthylacetic acid and sodium nitrophenolate can promote the growth of the root system of Chinese cabbage and increase the yield to varying degrees, and the effect of the ternary compound composition formula in this study is more obvious. Formula B has the best effect, and the total root length, total root surface area, total root volume and average root diameter of Chinese cabbage are increased by 23%, 20%, 71% and 57% compared with the control (water); in the control of using a single composition or a binary composition, the effect of control D is more obvious, and control D is 13%, 11%, 14% and 14% higher than the control (water) in the total root length, total root surface area, total root volume and average root diameter of Chinese cabbage. It shows that the ternary formula of the present invention is more effective than the single and binary formulas in the experiment, and can promote the growth and development of the root system of the plant.

[0067] Example 2: Indoor test of corn

[0068] The ingredients of the preparation are consistent with those in Example 1.

[0069] Test object: Corn (Zhengdan 958)

[0070] Test method: The test was conducted in the glass greenhouse of Shenyang Institute of Chemical Industry. Field soil was used for planting, 3 corns were sown in each pot, and corn seedlings with the same growth were selected for planting after the corn plants grew to 5 cm. The growth conditions of each pot of seedlings were kept consistent. After the seedlings were grown, the base fertilizer was applied, as was the preparation and control preparation in the above example. The base fertilizer was compound fertilizer 15-15-15, at a dosage of 600 kg / hm2. 2For application, the dosage of the preparation is 20 g added to 1 t of fertilizer. The active ingredients of the relevant ternary formula are fixed to volume with water according to the active ingredient ratio and concentration of formula B in Table 1, and the active ingredients of the binary formula are fixed to volume with water according to the active ingredient ratio and concentration of control D in Table 2. After mixing with the fertilizer, it is applied to the soil. The growth of the corn root system was investigated 30 days after application, with 5 replicates for each treatment. The test results are shown in Table 4.

[0071] Table 4: Corn pot test data

[0072]

[0073] As shown in Table 4, the results of the potted plant test show that persimmon tannin + naphthylacetic acid + sodium nitrophenolate and naphthylacetic acid + sodium nitrophenolate can promote the growth of corn roots and leaves to varying degrees, and the ternary compound of persimmon tannin + naphthylacetic acid + sodium nitrophenolate is more effective than the binary compound of naphthylacetic acid + sodium nitrophenolate. The ternary compound treatment increased the leaf length, leaf width and root length of corn by 40%, 22% and 28% respectively compared with the control (water); and increased them by 18%, 8% and 11% respectively compared with the binary compound treatment. This shows that the ternary formula is more effective than the binary formula, and can promote the growth and development of corn leaves and roots, laying the foundation for increasing yields. See attached pictures. Figure 1 .

[0074] Example 3: Corn Outdoor Trial

[0075] The ingredients of the preparation are consistent with those in Example 1.

[0076] Test object: Corn (Zhongdefeng No. 6)

[0077] Test method: The test was conducted at the corn experimental base in Jinzhong City, Shanxi Province. The test crop was corn (Zhongdefeng No. 6) and the test area was 2 mu. The fertilization method was mixed with base fertilizer. The base fertilizer was compound fertilizer 15-15-15, and the dosage was 675 kg / hm2. 2 Application, the dosage of the preparation is 20g in 1t fertilizer, the active ingredients of the relevant ternary formula are fixed to volume with water according to the active ingredient ratio and concentration of formula B in Table 1, and the active ingredients of the binary formula are fixed to volume with water according to the active ingredient ratio and concentration of control D in Table 2, mixed with fertilizer and applied to the soil. After maturity, the yield survey was carried out, and the sampling method was to take 2 points for each treatment, where the area of ​​each corn point was 2m×3 ridges, the ridge width was 0.50m, and the area was 3m 2 , the test results are shown in Table 5.

[0078] Table 5: Yield measurement data of corn with different recipes

[0079]

[0080] As shown in Table 5, the ternary formula of persimmon tannin + naphthylacetic acid + sodium nitrophenolate in the experiment improved the single cob weight, kernel weight per unit area, seed yield and yield per mu of corn to varying degrees compared with the binary formula of naphthylacetic acid + sodium nitrophenolate. The ear weight (with axis) of corn treated with the ternary formula increased by 8.32% compared with the binary formula; the kernel weight per unit area after threshing increased by 18.35%, the seed yield per unit area increased by 2.72%; and the yield per mu increased by 18.35%. This shows that the ternary formula can promote the growth of corn, increase the accumulation of substances, increase the kernel weight and seed yield per unit area of ​​corn, and thus increase the yield per mu of corn. This shows that the ternary formula is significantly more effective than the binary formula, and has an unexpectedly significant improvement effect. See attached for photos. Figure 2 .

[0081] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A plant growth regulator for promoting root growth and increasing production, characterized in that: The active ingredients of the growth regulator are active ingredient A, active ingredient B and active ingredient C; The active ingredient A is persimmon tannin, the active ingredient B is naphthylacetic acid, and the active ingredient C is sodium nitrophenolate; The concentration of active ingredient A is 0.1 mg / L, the concentration of active ingredient B is 1 mg / L, and the concentration of active ingredient C is 10 mg / L; The preparation method of active ingredient A persimmon tannin comprises the following steps: 1) Raw material processing: washing and crushing the persimmons; 2) Ultrahigh pressure treatment: adding purified water to the crushed persimmons in step 1), and then adjusting the pressure and the action temperature to perform ultrahigh pressure treatment; 3) Ultrasonic treatment: subjecting the solution obtained in step 2) to ultrasonic treatment; 4) Enzymatic hydrolysis: placing the solution obtained in step 3) in a sealed fermentation tank, adding activated complex enzymes of pectinase, apple amylase and pectin lyase for enzymatic hydrolysis; 5) filtering and concentrating the enzymatic hydrolyzate obtained in step 4), and spray drying to obtain persimmon tannin; adding 5-10 times the weight of pure water in step 2), the pressure is 350 MPa, the action temperature is 40-50° C., and the ultrahigh pressure treatment time is 2-5 min; In step 3), the ultrasonic treatment time is 5-10 min, the action temperature is 40-50° C., and the power is 300 W; In step 4), the enzymolysis time is 20-30 minutes, the enzymolysis temperature is 43-45° C., the weight ratio of pectinase, apple amylase and pectin lyase is 5:3:2, and the amount of complex enzyme added is 40-80 mg / kg.

2. The plant growth regulator for promoting root growth and increasing production according to claim 1, characterized in that: The dosage form of the plant growth regulator is one of aqueous solution, wettable powder, water dispersible granule, oil suspension, suspoemulsion, emulsifiable concentrate, microemulsion, emulsion in water, soluble granule or soluble powder.

3. A use of the plant growth regulator for promoting root growth and increasing yield according to claim 1 in regulating crop growth, characterized in that: The crops include leafy vegetables and cereal crops; The specific steps of the application are: S1: Select crops; S2: preparing ternary formulation ingredients; S3: Mix with base fertilizer for fertilization. The ternary formula components in S2 are fixed to volume with water, then mixed with base fertilizer and applied to the crop soil in S1; S4: After fertilizing in S3, wait for the crops to mature.

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