A pine wood nematode detection all-in-one machine

Abstract

The application relates to a detection device, and particularly discloses a pine wood nematode detection all-in-one machine. The pine wood nematode detection all-in-one machine comprises a sampling device, a processing device and a detection device, the processing device is arranged on the detection device, the sampling device is slidingly arranged on the processing device, a driving piece for driving the sampling device to slide is arranged on the processing device, and the driving piece is connected with the sampling device. The detection all-in-one machine can detect pine wood nematodes at any time and any place, is convenient and fast, and thus the detection efficiency is improved.

Description

Technical Field

[0001] This application relates to a detection device, and more particularly to an integrated machine for detecting pine wilt disease. Background Technology

[0002] Pine wilt nematode is a common parasite of pine trees. The adult nematode is about 1 mm long. The female's tail is nearly conical with a rounded end; the male's tail resembles a bird's claw and curves ventrally. It is transmitted by vector insects such as the pine sawyer beetle, causing pine wilt disease. Once infected, the needles turn yellowish-brown or reddish-brown, the entire tree dries up and dies, eventually rotting. Pine wilt nematode is usually detected using a PCR instrument. However, PCR instruments are typically placed in laboratories, requiring samples to be collected from the field, pre-treated, and then tested, which affects detection efficiency. Summary of the Invention

[0003] To improve the detection efficiency of pine wilt disease, this application provides an integrated pine wilt disease detection machine.

[0004] This application provides an integrated pine wilt nematode detection machine, which adopts the following technical solution:

[0005] A pine wilt disease detection integrated machine includes a sampling device, a processing device, and a detection device. The processing device is disposed on the detection device, and the sampling device is slidably disposed on the processing device. The processing device is provided with a driving component for driving the sampling device to slide, and the driving component is connected to the sampling device.

[0006] By adopting the above technical solution, the sampling device is first moved by the driving component to sample different parts of the pine wood. Then, the sample is pre-processed by the processing device. Finally, the pre-processed sample is tested by the detection device. Pine wood can be tested anytime and anywhere, which is convenient and fast, thereby improving the detection efficiency.

[0007] In one specific implementation, the processing device includes a processing chamber disposed on the detection device. The processing chamber has a processing channel communicating with the detection device. A first annular groove is formed on the peripheral wall of the processing channel. A first rotating ring is rotatably connected to the groove wall of the first annular groove. A first worm gear is connected to the outer wall of the first rotating ring. A first motor is mounted on the processing chamber. A first worm is coaxially connected to the motor shaft of the first motor and meshes with the first worm gear. A processing tube is disposed on the peripheral wall of the processing channel. An insertion block is disposed on the inner wall of the first rotating ring. A slot for inserting the insertion block is formed on the processing tube. A heating tube is installed inside the processing chamber.

[0008] By adopting the above technical solution, the obtained wood chip sample is added to the processing tube, which contains lyophilized lysis solution powder, and then water is added. The motor shaft of the first motor drives the first worm, the first worm wheel, and the first rotating ring to rotate in sequence. With the cooperation of the insert and the slot, the first rotating ring drives the processing tube to rotate centrifugally. While rotating, the heating tube heats the processing tube, first at 65°C for 25 minutes, and then at 95°C for 5 minutes. The processing tube is then pulled out, and the wood chips are separated to the bottom of the processing tube using an inner tube with a 500-mesh sieve. The upper clear liquid is poured into the detection device for detection.

[0009] In one specific implementation, a second annular groove communicating with the processing channel is formed on the top wall of the processing chamber, a second rotating ring is rotatably connected to the groove wall of the second annular groove, a locking block is connected to the processing tube, and a locking slot for the locking block to be inserted is formed on the second rotating ring, the locking slot being arranged opposite to the locking block.

[0010] By adopting the above technical solution, the cooperation of the card block and the card slot facilitates the rotation of the processing tube by the second rotating ring and the installation and removal of the processing tube. The setting of the second rotating ring improves the stability of the rotation of the processing tube. The card slot and the insertion block are set opposite to each other, which plays a certain guiding role and makes it easy to insert the insertion block into the slot.

[0011] In one specific implementation, the sampling device includes a lifting block and an electric drill that are slidably disposed on the processing chamber. A first rotating rod is hinged to the lifting block, a first telescopic rod is slidably connected to the first rotating rod, a second rotating rod is hinged to the first telescopic rod, a second telescopic rod is slidably connected to the second rotating rod, a sampling tube is rotatably disposed on the second telescopic rod, and an adsorption element for adsorbing the sawdust in the sampling tube is disposed on the processing chamber.

[0012] By adopting the above technical solution, the sampling tube is placed against the pine wood, and then an electric drill is used to drill. During the drilling process, the adsorption component is used to suck up the sawdust in the sampling tube. The cooperation of the first rotating rod, the first telescopic rod, the second rotating rod, and the second telescopic rod makes it convenient to sample different positions at the same height of the pine wood.

[0013] In one specific implementation, the adsorption element includes an exhaust fan installed on the processing chamber. The air inlet of the exhaust fan is connected to a feed hose, and the air outlet of the exhaust fan is connected to a discharge hose. The discharge hose is inserted into the processing tube. A connecting pipe is connected to the sampling tube, and a protruding ring is provided on the connecting pipe. The feed hose is sleeved on the connecting pipe.

[0014] By adopting the above technical solution, the drilled wood chips are sucked into the processing pipe by a blower; the connection between the connecting pipe and the convex ring facilitates the installation and removal of the feed hose and the sampling pipe.

[0015] In one specific implementation, a first sliding groove is formed on the end wall of the first rotating rod, and a first limiting groove is formed on the groove wall of the first sliding groove. The first telescopic rod is slidably connected to the groove wall of the first sliding groove, and a first limiting block is connected to the first telescopic rod, which is slidably connected to the groove wall of the first limiting groove. A second sliding groove is formed on the end wall of the second rotating rod, and a second limiting groove is formed on the groove wall of the second sliding groove. The second telescopic rod is slidably connected to the groove wall of the second sliding groove, and a second limiting block is connected to the second telescopic rod, which is slidably connected to the groove wall of the second limiting groove.

[0016] By adopting the above technical solution, the stability of the sliding of the first telescopic rod is improved by utilizing the cooperation of the first limiting block and the first limiting groove, and the first telescopic rod can also be prevented from sliding out of the first sliding groove; by utilizing the cooperation of the second limiting block and the second limiting groove, the stability of the sliding of the second telescopic rod is improved, and the second telescopic rod can also be prevented from sliding out of the second sliding groove.

[0017] In one specific implementation, the drive component includes a threaded rod rotatably connected to the processing chamber, the threaded rod being threadedly connected to the lifting block, a second worm gear being connected to the threaded rod, a second motor being mounted on the processing chamber, a second worm coaxially connected to the motor shaft of the second motor, the second worm meshing with the second worm gear, a guide rod being connected to the processing chamber, and a guide groove being provided on the lifting block for the guide rod to be inserted.

[0018] By adopting the above technical solution, the motor shaft of the second motor drives the second worm, the second worm wheel, and the threaded rod to rotate in sequence. With the cooperation of the guide rod and the guide groove, the lifting block has a certain limiting effect, so that the threaded rod drives the lifting block to slide and completes the sliding adjustment of the lifting block.

[0019] In one specific implementation, the detection device is provided with a fixing device for connecting with pine wood. The detection device has a third sliding groove. The fixing device includes a bidirectional screw rotatably connected to the wall of the third sliding groove. Two clamping blocks are threadedly connected to the bidirectional screw. The clamping blocks are slidably connected to the wall of the third sliding groove. Curved surfaces are provided on the opposite side walls of the two clamping blocks, and connecting straps are provided on the two clamping blocks. The two connecting straps are connected by buckles.

[0020] By adopting the above technical solution, rotating the bidirectional screw drives two clamping blocks to clamp the pine material, and then connecting the two clamping blocks with a connecting belt, thereby fixing the detection device on the pine material; the curved surface design increases the contact area between the clamping blocks and the pine material, thereby further improving the connection strength between the detection device and the pine material.

[0021] In summary, this application includes at least one of the following beneficial technical effects:

[0022] 1. In this application, the sampling device is first moved by the driving component to sample different parts of the pine wood. Then, the sample is pre-processed by the processing device. Finally, the pre-processed sample is tested by the detection device. Pine wood can be tested anytime and anywhere, which is convenient and fast, thereby improving the detection efficiency.

[0023] 2. In this application, the obtained wood chip sample is added to the processing tube, which contains lyophilized lysis solution powder, and then water is added. The motor shaft of the first motor drives the first worm, the first worm wheel, and the first rotating ring to rotate in sequence. With the cooperation of the insert and the slot, the first rotating ring drives the processing tube to rotate centrifugally. While rotating, the heating tube heats the processing tube. First, it is heated at 65°C for 25 minutes, and then at 95°C for 5 minutes. The processing tube is then pulled out, and the wood chips are separated to the bottom of the processing tube using an inner tube with a 500-mesh sieve. The upper clear liquid is poured into the detection device for detection.

[0024] 3. The sampling device in this application places the sampling tube against the pine wood and then drills it with an electric drill. During the drilling process, the adsorption element is used to suck up the wood chips in the sampling tube. The cooperation of the first rotating rod, the first telescopic rod, the second rotating rod, and the second telescopic rod makes it convenient to sample different positions at the same height of the pine wood. Attached Figure Description

[0025] Figure 1 This is a schematic diagram illustrating the overall structure of the integrated testing machine in the embodiments of this application.

[0026] Figure 2 This is a cross-sectional schematic diagram illustrating the processing device in the embodiments of this application.

[0027] Figure 3 This is a schematic diagram illustrating the structure of the sampling device in the embodiments of this application.

[0028] Figure 4 This is an exploded schematic diagram illustrating the connection between the sampling tube and the feed hose in an embodiment of this application.

[0029] Figure 5 This is a cross-sectional schematic diagram used to illustrate the driving component in the embodiments of this application.

[0030] Figure 6 This is a cross-sectional schematic diagram used to illustrate the sampling device in the embodiments of this application.

[0031] Figure 7 yes Figure 4 Enlarged view of point A in the middle.

[0032] Explanation of reference numerals in the attached drawings: 1. Sampling device; 11. Lifting block; 111. Guide groove; 12. First rotating rod; 121. First sliding groove; 122. First limiting groove; 13. First telescopic rod; 131. First limiting block; 14. Second rotating rod; 141. Second sliding groove; 142. Second limiting groove; 15. Second telescopic rod; 151. Second limiting block; 16. Sampling tube; 161. Connecting tube; 162. Protruding ring; 2. Processing device; 21. Processing chamber; 211. Processing channel; 212. First annular groove; 213. Second annular groove; 22. First rotating ring; 23. ... 1. Worm gear; 24. First motor; 25. First worm; 26. Processing tube; 261. Slot; 262. Clamping block; 27. Insertion block; 28. Second rotating ring; 281. Slot; 3. Detection device; 31. Chassis; 311. Third sliding groove; 4. Drive component; 41. Threaded rod; 42. Second worm gear; 43. Second motor; 44. Second worm; 45. Guide rod; 5. Fixing device; 51. Bidirectional screw; 52. Clamping block; 521. Curved surface; 53. Connecting belt; 54. Buckle; 6. Adsorption component; 61. Exhaust fan; 62. Feed hose; 63. Discharge hose. Detailed Implementation

[0033] The following is in conjunction with the appendix Figure 1-7 This application will be described in further detail.

[0034] This application discloses an integrated machine for detecting pine wilt disease.

[0035] Reference Figure 1 A pine wilt nematode detection integrated machine includes a sampling device 1, a processing device 2, and a detection device 3. The processing device 2 is mounted on the detection device 3, the sampling device 1 is slidably mounted on the processing device 2, the processing device 2 is provided with a driving component 4, the driving component 4 is connected to the sampling device 1, and the detection device 3 is provided with a fixing device 5.

[0036] Reference Figure 1The detection device 3 includes a chassis 31, in which a PCR detector (not shown in the figure) is installed. A third sliding groove 311 is provided on the outer wall of the chassis 31. The fixing device 5 includes a bidirectional screw 51 rotatably connected between two opposite end walls of the third sliding groove 311. The bidirectional screw 51 is horizontally set and one end extends out of the third sliding groove 311. Two clamping blocks 52 are threadedly connected to the bidirectional screw 51. The clamping blocks 52 are slidably connected to the groove wall of the third sliding groove 311. Curved surfaces 521 are provided on the opposite side walls of the two clamping blocks 52, and connecting straps 53 are installed on the two clamping blocks 52. The two connecting straps 53 are connected by buckles 54.

[0037] Reference Figure 2 and Figure 3 The processing device 2 includes a processing chamber 21 mounted on the top wall of the outer casing 31. A processing channel 211 is formed inside the processing chamber 21. The processing channel 211 is vertically arranged and connected to the feed end of the PCR detector. A first annular groove 212 is formed on the peripheral wall of the processing channel 211. A first rotating ring 22 is rotatably connected to the wall of the first annular groove 212. The first rotating ring 22 is horizontally arranged, and a first worm gear 23 is installed on the outer wall of the first rotating ring 22. A first motor 24 is installed on the outer wall of the processing chamber 21. The first motor 24 is horizontally arranged, and a first worm gear 25 is coaxially fixedly connected to the motor shaft of the first motor 24. The first worm gear 25 meshes with the first worm gear 23. The peripheral wall of the processing channel 211... A processing tube 26 is provided on the upper part of the processing chamber 21, which is inserted into the processing channel 211. An insert 27 is integrally formed on the inner wall of the first rotating ring 22. A slot 261 is provided on the processing tube 26, and the insert 27 is inserted into the slot 261 and abuts against the slot wall. A second annular groove 213 is provided on the top wall of the processing chamber 21, which communicates with the processing channel 211. A second rotating ring 28 is rotatably connected to the wall of the second annular groove 213. The second rotating ring 28 is horizontally positioned. A locking block 262 is fixedly connected to the processing tube 26. A locking groove 281 is provided on the second rotating ring 28, and the locking block 262 is inserted into the locking groove 281 and abuts against the groove wall. The locking groove 281 is positioned opposite to the insert 27. A heating tube (not shown in the figure) is installed inside the processing chamber 21.

[0038] Reference Figure 4 and Figure 5The sampling device 1 includes an electric drill (not shown in the figure) and a lifting block 11 that is slidably mounted on the top wall of the processing chamber 21. The driving component 4 includes a threaded rod 41 that is rotatably connected to the top wall of the processing chamber 21. The threaded rod 41 is vertically mounted and threadedly connected to the lifting block 11. A second worm gear 42 is fixedly connected to the threaded rod 41. A second motor 43 is mounted on the outer wall of the processing chamber 21. The second motor 43 is horizontally mounted and a second worm gear 44 is coaxially fixedly connected to the motor shaft of the second motor 43. The second worm gear 44 meshes with the second worm gear 42. A guide rod 45 is fixedly connected to the outer top wall of the processing chamber 21. The guide rod 45 is vertically mounted. A guide groove 111 is opened on the bottom wall of the lifting block 11. The guide rod 45 is inserted into the guide groove 111 and abuts against the groove wall of the guide groove 111.

[0039] Reference Figure 3 and Figure 6 A first rotating rod 12 is hinged to the side wall of the lifting block 11. A first sliding groove 121 is formed on the end wall of the first rotating rod 12 away from the lifting block 11. A first limiting groove 122 is formed on the groove wall of the first sliding groove 121. A first telescopic rod 13 is slidably connected to the groove wall of the first sliding groove 121. A first limiting block 131 is fixedly connected to the first telescopic rod 13. The first limiting block 131 is slidably connected to the groove wall of the first limiting groove 122. One end of the first telescopic rod 13 extends out of the first sliding groove 121 and the end... A second rotating rod 14 is hinged to the wall. A second sliding groove 141 is provided on the end wall of the second rotating rod 14 away from the first telescopic rod 13. A second limiting groove 142 is provided on the groove wall of the second sliding groove 141. A second telescopic rod 15 is slidably connected to the groove wall of the second sliding groove 141. A second limiting block 151 is fixedly connected to the second telescopic rod 15. The second limiting block 151 is slidably connected to the groove wall of the second limiting groove 142. A sampling tube 16 is hinged to the end wall of the second telescopic rod 15 away from the second rotating rod 14.

[0040] Reference Figure 3 and Figure 7 An adsorption component 6 is provided on the top wall of the processing chamber 21. The adsorption component 6 includes an exhaust fan 61 installed on the top wall of the processing chamber 21. The air inlet end of the exhaust fan 61 is fixedly connected to a feed hose 62, and the air outlet end of the exhaust fan 61 is fixedly connected to a discharge hose 63. The end of the discharge hose 63 away from the exhaust fan 61 is inserted into the processing tube 26. A connecting pipe 161 is provided on the sampling tube 16. A protruding ring 162 is integrally formed on the outer peripheral wall of the connecting pipe 161. The feed hose 62 is sleeved on the connecting pipe 161.

[0041] The implementation principle of the pine wood nematode detection integrated machine in this application embodiment is as follows: First, rotate the bidirectional screw 51, and the two clamping blocks 52 move closer to each other to clamp the pine wood. Then, connect the two clamping blocks 52 with the connecting belt 53 to fix the machine box 31 on the pine wood. The second motor 43 can be used to drive the second worm 44, the second worm wheel 42, and the threaded rod 41 to rotate in sequence. The threaded rod 41 drives the lifting block 11 to slide. Then, rotate the first rotating rod 12 and the second rotating rod 14 to adjust the sampling position of the sampling tube 16 and place the sampling tube 16 against the pine wood. Then, use an electric drill to drill. During the drilling process, use an exhaust fan 61 to suck in the drilled wood chips. In the processing tube 26, lysate powder of lysis buffer is reserved, and then water is added. The motor shaft of the first motor 24 drives the first worm 25, the first worm wheel 23, and the first rotating ring 22 to rotate in sequence. With the cooperation of the insert block 27 and the slot 261, the first rotating ring 22 drives the processing tube 26 to rotate and centrifuge. While rotating, the heating tube heats the processing tube 26. First, it is heated at 65°C for 25 minutes, and then at 95°C for 5 minutes. The processing tube 26 is then pulled out. Then, the sawdust is separated to the bottom of the processing tube 26 using an inner tube with a 500-mesh sieve. The supernatant is poured into the PCR detector through the processing channel 211 for detection.

[0042] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A pine wilt nematode detection integrated machine, characterized in that: The device includes a sampling device (1), a processing device (2), and a detection device (3). The processing device (2) is mounted on the detection device (3). The sampling device (1) is slidably mounted on the processing device (2). The processing device (2) is provided with a driving member (4) for driving the sampling device (1) to slide. The driving member (4) is connected to the sampling device (1). The processing device (2) includes a processing chamber (21) mounted on the detection device (3). The processing chamber (21) has a processing channel (211) that communicates with the detection device (3). A first annular groove (212) is formed on the peripheral wall of the processing channel (211). A first rotating ring (22) is rotatably connected to the wall, and a first worm gear (23) is connected to the outer wall of the first rotating ring (22). A first motor (24) is installed on the processing chamber (21), and a first worm (25) is coaxially connected to the motor shaft of the first motor (24). The first worm (25) meshes with the first worm gear (23). A processing tube (26) is provided on the peripheral wall of the processing channel (211). An insert (27) is provided on the inner wall of the first rotating ring (22), and a slot (261) for inserting the insert (27) is provided on the processing tube (26). A heating tube is installed inside the processing chamber (21). The sampling device (1) includes a component that is slidably disposed in the processing chamber. (21) The lifting block (11) and the electric drill are mounted on the lifting block (11). A first rotating rod (12) is hinged on the lifting block (11). A first telescopic rod (13) is slidably connected to the first rotating rod (12). A second rotating rod (14) is hinged on the first telescopic rod (13). A second telescopic rod (15) is slidably connected to the second rotating rod (14). A sampling tube (16) is rotatably mounted on the second telescopic rod (15). An adsorption element (6) for adsorbing the sawdust in the sampling tube (16) is provided on the processing chamber (21). A first sliding groove (121) is provided on the end wall of the first rotating rod (12). A first limiting groove (122) is provided on the groove wall of the first sliding groove (121). The first telescopic rod (13) is slidably connected to the groove wall of the first sliding groove (121), and a first limiting block (131) is connected to the first telescopic rod (13). The first limiting block (131) is slidably connected to the groove wall of the first limiting groove (122). A second sliding groove (141) is provided on the end wall of the second rotating rod (14), and a second limiting groove (142) is provided on the groove wall of the second sliding groove (141). The second telescopic rod (15) is slidably connected to the groove wall of the second sliding groove (141), and a second limiting block (151) is connected to the second telescopic rod (15). The second limiting block (151) is slidably connected to the groove wall of the second limiting groove (142).The driving component (4) includes a threaded rod (41) rotatably connected to the processing chamber (21), the threaded rod (41) being threadedly connected to the lifting block (11), a second worm gear (42) connected to the threaded rod (41), a second motor (43) mounted on the processing chamber (21), a second worm (44) coaxially connected to the motor shaft of the second motor (43), the second worm (44) meshing with the second worm gear (42), a guide rod (45) connected to the processing chamber (21), and a guide groove (111) provided on the lifting block (11) for the guide rod (45) to be inserted.

2. The pine wilt nematode detection integrated machine according to claim 1, characterized in that: The top wall of the processing chamber (21) is provided with a second annular groove (213) that communicates with the processing channel (211). A second rotating ring (28) is rotatably connected to the groove wall of the second annular groove (213). A locking block (262) is connected to the processing tube (26). A slot (281) for inserting the locking block (262) is provided on the second rotating ring (28). The slot (281) is arranged opposite to the insertion block (27).

3. The pine wilt nematode detection integrated machine according to claim 1, characterized in that: The adsorption component (6) includes a blower (61) installed on the processing chamber (21). The air inlet of the blower (61) is connected to a feed hose (62), and the air outlet of the blower (61) is connected to a discharge hose (63). The discharge hose (63) is inserted into the processing tube (26). A connecting pipe (161) is connected to the sampling tube (16). A protruding ring (162) is provided on the connecting pipe (161). The feed hose (62) is sleeved on the connecting pipe (161).

4. The pine wilt nematode detection integrated machine according to claim 1, characterized in that: The detection device (3) is provided with a fixing device (5) for connecting with pine wood. The detection device (3) is provided with a third sliding groove (311). The fixing device (5) includes a bidirectional screw (51) rotatably connected to the groove wall of the third sliding groove (311). Two clamping blocks (52) are threadedly connected to the bidirectional screw (51). The clamping blocks (52) are slidably connected to the groove wall of the third sliding groove (311). Curved surfaces (521) are provided on the opposite side walls of the two clamping blocks (52). Connecting straps (53) are provided on the two clamping blocks (52). The two connecting straps (53) are connected by buckles (54).

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