Anti-hair loss and hair growth cream containing coffee bean fermentation liquid Supferm COFE and vitality coffee and preparation method thereof

Abstract

The present invention discloses an anti-hair loss and hair-producing water cream containing coffee bean fermentation liquid Supferm COFE and Starfact HCS and a preparation method thereof. The water-producing cream formula includes, by mass percentage, an emulsifier, a conditioner, a moisturizer, a preservative, and an active substance. The active substances include Supferm COFE, Starfact HCS, and Starfact HCS 50C, and the remainder is deionized water. The anti-hair loss and hair-producing water cream has a light coffee frosty appearance and a chewy jelly texture. When applied to the skin, the frosty paste quickly breaks the emulsion and releases fine small water droplets, producing a strong water feeling, giving the user a hydrated, refreshing, and moisturizing pleasant skin feeling. The resident product of the oil-in-water system can better exert the role of the active ingredients, and the unique skin feeling makes consumers happy to use it without feeling burdened.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics, and specifically relates to an anti-hair loss and hair growth water cream containing Supferm COFE Vitality Coffee 50C and a preparation method thereof. Background Art

[0002] In recent years, hair loss prevention and hair growth have become a hot topic. According to the latest statistics from the World Health Organization, one in six Chinese people has symptoms of hair loss, and there is a trend of younger age. Young and middle-aged people have become the main force of the "hair loss army". The latest survey shows that 60% of men begin to lose their hair before the age of 25, and nearly 84% begin to lose their hair before the age of 30. The market share of online hair loss prevention and hair growth products continues to increase, with a growth rate of 40% from 2017 to 2019. At present, common causes of hair loss include androgenic alopecia, endocrine alopecia, neurotic alopecia, physical alopecia, etc. Consumers believe that products that can clearly feel the reduction of hair breakage and hair loss can bring them a satisfactory experience. Faced with such market and consumer demand, research and development of formulas with hair growth functions have broad market prospects.

[0003] As consumers have a high level of demand for hair products and pay more and more attention to health, the proportion of products with scalp care claims has gradually increased, becoming the focus of people's attention. For scalp care products, people are not only concerned about the efficacy, but also pay more and more attention to the safety and mildness of the products. The consumption concept has gradually developed to pursue the desired efficacy based on the safety and mildness of the products.

[0004] The number of hair products claiming to be "scalp care" on the market has been increasing year by year. According to their different performance, scalp care products are mainly divided into two categories. One is the wash-off scalp care products, which are mainly used for hair washing and scalp care. By using mild surfactants, special oils, natural active ingredients and other ingredients, the hair is better conditioned and has a certain care effect on the scalp. The other is the stay-on scalp care products, which are mainly applied to the scalp and slowly massaged until absorbed, thereby improving scalp problems. This type of product mainly achieves the purpose of improving scalp health by adding effective ingredients.

[0005] This formula is mainly composed of active ingredients with hair growth and development effects. It stimulates hair follicle growth by activating the activity of hair follicle stem cells, inhibiting the expression of TGF-β1, inhibiting tyrosinase, etc., preventing the growth follicles from entering the decline stage too early, preventing premature hair loss and promoting hair growth. Summary of the invention

[0006] The invention discloses a novel scalp water-removing cream formula. Products suitable for scalp care generally need to be very refreshing. Generally speaking, scalp care products are not used. With the improvement of living standards and people's awareness of scalp care, more and more people start to care for their scalp from a very young age. Scalp care products (retention type) on the market are generally aqueous or water-in-oil type formulations, and their effects are mainly moisturizing, anti-dandruff, anti-itching, oil control, anti-allergic and other effects. However, there are not many products for stimulating hair follicle vitality to prevent premature hair loss and promote hair growth, and there are even fewer oil-in-water type products. The present invention provides a water-removing cream formula containing Supferm COFE and Starfact HCS, which is helpful for preventing hair loss and promoting hair growth, fundamentally preventing premature hair loss and promoting hair growth. Supferm COFE and Starfact HCS are both prepared by Zhejiang Chenhai Life Sciences Co., Ltd., and Starfact HCS is represented by Guangzhou Shengkais Chemical Technology Co., Ltd.

[0007] Pure caffeine is a white powder. Due to its poor water solubility, it is difficult to use in the formulation. In the prior art, caffeine is generally not directly used as an additive. Because it is not water-soluble, it cannot be absorbed by the skin. Caffeine is a highly effective active substance, but it has the following three drawbacks that cannot be overcome: 1) low solubility limits the dosage; 2) it is cytotoxic at high dosages; 3) the skin has low bioavailability. Therefore, how to keep caffeine active while being absorbed by the skin, it is very important to use cyclodextrin to encapsulate caffeine. The present invention mainly improves the problem of low water solubility of caffeine and the size of the carrier. In this application, nano-scale inclusion β-cyclodextrin is used as a carrier, and non-water-soluble caffeine is nano-encapsulated with β-cyclodextrin, so that caffeine can be well used in cosmetics and personal care products. In addition, it is also necessary to control the size of the carrier to achieve the addition of caffeine and efficient attachment to the skin surface of the scalp without penetrating into the skin.

[0008] The Vitality Coffee 50C in the present invention is 50% caffeine made by using cyclodextrin encapsulation technology (CycloXome technology), making it more soluble in water, thus achieving the purpose of using caffeine in cosmetics.

[0009] The purpose of the present invention is achieved by at least one of the following technical solutions.

[0010] A hair loss prevention and hair growth water cream containing coffee bean fermentation liquid Supferm COFE and Vitality Coffee, which comprises, by mass percentage, 1% to 3% of emulsifier, 6% to 12% of conditioner, 0.5% to 2% of preservative, 10% to 15% of moisturizer, 5% to 35% of Supferm COFE, 0.5% to 5% of Starfact HCS and 1% to 3% of Vitality Coffee 50C, and the remainder is deionized water;

[0011] The water-removing cream formula is a water-removing cream for scalp care, hair loss prevention and hair growth;

[0012] In the present invention, the active ingredient is one or more of Supferm COFE, Starfact HCS, and Vitality Coffee 50C.

[0013] The active ingredients are SupfermCOFE, a skin-refreshing coffee prepared by Zhejiang Chenhai Life Sciences Co., Ltd., and 50C, a vitality coffee represented by Guangzhou Shengkais Chemical Technology Co., Ltd.; the main function of caffeine is to promote hair growth. Caffeine can inhibit the activity of 5α-reductase and restore the hair to the growth phase; it can also promote hair growth by inhibiting PDE enzymes and promoting microcirculation.

[0014] The Starfact HCS is composed of hydrolyzed collagen PG-propylmethylsilanediol and water, wherein the mass percentage of hydrolyzed collagen PG-propylmethylsilanediol is 10%, and the remaining 90% is water.

[0015] In the present invention, Supferm COFE is composed of yeast fermentation product, yeast fermentation product filtrate, Arabica coffee seed extract, 1,3-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone; wherein the mass fraction of the yeast fermentation product is 57.0%, the mass fraction of the yeast fermentation product filtrate is 35.0%, the mass fraction of Arabica coffee seed extract is 1.0%, the mass fraction of 1,3-propylene glycol is 6.0%, the mass fraction of 1,2-hexanediol is 0.5% and the mass fraction of p-hydroxyacetophenone is 0.5%; the skin-awakening coffee Supferm COFE is 1.0 ... The preparation method of COFE is as follows: prepare fermentation bacteria yeast and culture medium raw material green coffee beans, grind the coffee beans into powder, and prepare an aqueous solution with water, wherein the mass ratio of coffee powder to water is 1:100-5:100, sterilize at 100°C for 30 minutes, inoculate with bacterial liquid, add 1.0%-5.0% of yeast liquid, aerobically ferment (rotation speed is 150-200rpm / min) for 50 hours, centrifuge the fermented liquid at 4500rpm for 30 minutes, sterilize at 100°C for 30 minutes, remove impurities, take the filtrate and add 1,3-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone at 75°C, and obtain the product by fine filtration.

[0016] The preparation method of the skin-awakening coffee Supferm COFE is to use a specific substrate as a growth matrix, use a specific microorganism, and under specific culture conditions, allow the enzymes of the microorganisms during the growth process to convert the active ingredients in the substrate, and at the same time, the substrate provides the microorganisms with the substances required for growth, thereby converting the secondary metabolites of the microorganisms. The raw materials of this method can reach 100% bio-based, which means that all the raw materials are derived from organic matter in nature, and no raw materials need to be synthesized by chemical synthesis. In the method, the strains will produce a large number of enzymes, such as amylase, glycosidase, etc., during the metabolism process, and the substrate can be modified with the help of these metabolic enzymes, including: 1. Most of the active substances of plants exist in cells, and the cell wall is the biggest obstacle to extracting plant active substances. During the fermentation period, metabolic enzymes are used to break the cell wall, so as to efficiently obtain the active ingredients in the substrate 2. Make the effective ingredients small molecules, which can be quickly absorbed. The human body cannot directly use macromolecular substances such as proteins, polypeptides, polysaccharides, etc. to decompose them into small molecules through the action of multiple enzymes of microorganisms, which is more conducive to skin absorption and enhancement of efficacy and activity. 3. And the molecular weight and range of the target molecule can be controlled. The main function of Supferm COFE here is anti-oxidation, making scalp cells more active.

[0017] The preparation process of Vitality Coffee 50C is as follows: dissolving synthetic caffeine in an ethanol solution, adding cyclodextrin, stirring for 45 to 55 hours, evaporating to complete dryness under vacuum at a temperature of 48 to 52° C., grinding into powder, homogenizing, and sieving to obtain the target product; the mass ratio of the added amount of synthetic caffeine to cyclodextrin is 1:0.9 to 1.

[0018] The Vitality Coffee 50C includes an inclusion and a coating, wherein the inclusion is caffeine, and the coating is a cylindrical nanomaterial cyclodextrin, and the inner cavity diameter of the coating is less than 1nm. Caffeine is wrapped in cyclodextrin and will not penetrate into the skin but can adhere to the skin and have a sustained release effect, slowly releasing the active substance on the skin surface, having a slow and continuous release effect, protecting the active molecule caffeine, such as Figure 5a shown.

[0019] Its sustained release mechanism is:

[0020] 1. When applied to the skin, only a small amount of encapsulated molecules are released;

[0021] 2. Establish thermodynamic equilibrium between intact Vitality Coffee 50C, free active ingredients and unencapsulated cyclodextrin;

[0022] 3. As free active substances penetrate into the skin, their concentration on the skin surface will decrease;

[0023] 4. The reduction of active molecules will induce more active molecules to be released from Vitality Coffee 50C.

[0024] Re-adjust the balance, such as Figure 5b As shown;

[0025] The cyclodextrin includes one or more combinations of α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, methyl cyclodextrin, hydroxypropyl cyclodextrin, and cyclodextrin laurate.

[0026] However, the bioavailability of caffeine to the skin is limited. The present invention uses a cylindrical nano-cyclodextrin to load caffeine, which can prevent the active factor from being lost during the transmission process; the main component of cyclodextrin is cyclopolyglucose, which is a conical cylindrical hollow three-dimensional structure. The upper end of the outer side is a hydrophobic structure formed by a C2-3 secondary hydroxyl structure, and the lower end is a hydrophilic structure formed by a C6 primary hydroxyl structure. Through the shielding effect of carbon-hydrogen bonds, a hydrophobic cavity is formed. The outer surface of this cylinder is polar, and the inner cavity is non-polar. Cyclodextrin wraps the non-polar molecules in the hydrophobic cavity, that is, the purpose of wrapping the active substance is completed. Specifically, the present invention utilizes the characteristics of molecular polar structure. After contacting the skin, the wrapped caffeine molecules are replaced by skin lipids, and are slowly and continuously released to penetrate into the lower part of the skin epidermis. The progressive release mechanism significantly improves the absorption rate of caffeine by the skin epidermis, and the active function of caffeine can be exerted for a long time. Acting on the scalp can effectively eliminate edema, promote moisture metabolism and blood circulation at the bottom of the muscle, and improve the condition of the scalp skin. In this application, cyclodextrin and caffeine are bound together by van der Waals forces. When the physical and chemical conditions change, they have the effect of slowly releasing active substances. Since water molecules are polar, they will occupy the inner cavity of cyclodextrin and be in a disordered state. At this time, the compound has a high entropy value. When non-polar molecules are miscible with cyclodextrin, they will automatically replace water molecules, changing from a high entropy value to a low entropy value, and becoming an ordered state, thereby forming a compound with a lower energy level, so a stable encapsulation component is formed. Because it is highly hydrophilic, cyclodextrin will not pass through the skin barrier, so the active substance will only be transmitted to the skin surface. After contacting the skin, the encapsulated active substance will be replaced by skin lipids and slowly and continuously released from cyclodextrin.

[0027] In the present invention, Caffenergia 50C, whose English name is CAFFENERGY 50C, can promote blood microcirculation, eliminate fat and resist oxidation, activate the activity of hair follicle stem cells, inhibit the expression of TCF-β1, inhibit tyrosinase, etc., thereby stimulating the growth of hair follicles, preventing the growth period hair follicles from entering the decline period too early, preventing premature hair loss and promoting hair growth.

[0028] In the present invention, Vitality Coffee 50C can increase the bioavailability. Vitality Coffee 50C in vitro skin absorption test shows that Figure 1 As shown, the caffeine in Vitality Coffee 50C stays on the skin's surface longer, increasing the long-lasting effect of the formula and the overall bioavailability of the caffeine.

[0029] In the present invention, Starfact HCS prepared by Zhejiang Chenhai Life Sciences Co., Ltd. is a modified product of keratin hydrolyzate that can moisturize and condition skin and hair, and has the advantages of organosiloxane, and is derived from pure wool. The present invention controls the molecular weight range of 3000-4000 Daltons of polypeptides. There is a high proportion of cystine-derived amino acids as part of the polypeptide skeleton. These amino acids provide measurable antioxidant activity, which can protect skin and hair, prevent environmental stress, inhibit protein and lipid oxidative damage, such as scavenging free radicals generated by sunlight.

[0030] Molecular weight is key to product performance, governing the ability to bind water, the extent of film formation and the ability to penetrate into the cortex of the hair fiber. Figure 7 Starfact HCS molecular weight distribution is shown.

[0031] More preferably, the emulsifier is one or more of SSG-210SP (dimethicone PEG-10 / 15 crosspolymer / dimethicone) and KF-6038 (PEG-9 polydimethylsiloxyethyl dimethicone).

[0032] More preferably, the conditioning agent includes at least one of isohexadecane, cyclopentasiloxane, polydimethylsiloxane, vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane crosspolymer, sodium chloride, and sodium citrate.

[0033] More preferably, the moisturizing agent is one or more of glycerin, butylene glycol, an amino acid moisturizing agent, trehalose, and 1,2-hexanediol.

[0034] More preferably, the preservative is p-hydroxyacetophenone.

[0035] The present invention discloses a method for preparing a water-derived cream for scalp care for preventing hair loss and promoting hair growth, containing Supferm COFE and Vitality Coffee 50C, comprising the following steps:

[0036] Step 1: After wetting vinyl dimethicone / polymethicone silsesquioxane crosspolymer and cyclopentasiloxane, add emulsifier and other conditioning agent components, and homogenize at high speed, with a homogenizing force of 3000-8000 r / min and a homogenizing time of 3 min-10 min to obtain component A;

[0037] Step 2: Stir p-hydroxyacetophenone, 1,2-hexanediol and 1,3-butanediol and heat to 70-80°C until transparent, add other moisturizer components and water and stir at 500-700 r / min for 1 minute until uniform, to obtain component B;

[0038] Step 3: Heat the ratio of Vitality Coffee 50C to water at 1:20 to 70-80°C, dissolve it, add it to component B, stir at 500-700r / min for 1 minute, cool it to below 45°C, add Supferm COFE and Starfact HCS, stir at 500-700r / min for 1 minute until it is uniform, and obtain component C.

[0039] Step 4: Slowly add C to A while stirring. After adding C, homogenize at a low speed of 2000-3000 r / min for about 2 minutes, and then stir at 500-700 r / min for 10 minutes until the paste has a thick texture, and the target product can be obtained.

[0040] The water cream formula of the present invention has certain considerations in terms of the safety of raw material selection and the overall matching of the formula. Compared with the prior art, the advantages of the present invention are:

[0041] The uniqueness of the present invention lies in that it is safe and reliable in terms of raw material selection. Furthermore, the present invention has a unique combination in terms of formula design: first, the present invention selects a silicone oil-in-water emulsifier, and the formula system based on silicone oil has a fresher skin feel and better spreadability than the traditional oil-in-water system. The oil-in-water retention product can better play the role of the active ingredients; second, the light yellow appearance of the present invention is a frosty appearance with a Q-elastic texture. When applied to the skin, the frosty paste quickly breaks the emulsion and releases fine small water droplets, producing a strong sense of water, giving the user a refreshing and moisturizing pleasant skin feeling; third, the present invention uses a combination of two caffeine-containing raw materials, and the Supferm COFE extracted from biological fermentation has a high content of chlorogenic acid, which mainly plays an antioxidant and anti-aging role. In the present invention, 30% Supferm COFE was tested. The DPPH free radical scavenging effect of COFE reached 93.93% under the condition of 50% test concentration, which is much better than the samples without adding or adding 5% SupfermCOFE. The Vitality Coffee 50C made by CycloXome technology is non-cytotoxic at a dosage of 1%, and can be released slowly and continuously on the skin for a long time. Compared with free caffeine, its bioavailability is improved. The main function of caffeine is to promote hair growth. Caffeine can inhibit the activity of 5α-reductase and restore the hair to the growth period. It can also promote hair growth by inhibiting PDE enzyme and promoting microcirculation.

[0042] Therefore, whether from the active raw materials selected for efficacy expression or from the overall combination of formula design, the water cream formula of the present invention has good skin feel, good efficacy, safety, is skin-friendly and non-toxic, the preparation process is not difficult to operate, it is suitable for a wide range of people, and can be well promoted. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1This is the in vitro skin absorption test chart of caffeine and Vitality Coffee 50C;

[0044] Figure 2 This is the in vitro cytotoxicity test result of Vitality Coffee 50C;

[0045] Figure 3a This is a diagram showing the effect of using the product of Example 1;

[0046] Figure 3b This is a diagram showing the effect of using the product of Example 1;

[0047] Figure 3c This is a diagram showing the effect of using the product of Example 1;

[0048] Figure 4a This is the blood flow velocity diagram before use;

[0049] Figure 4b This is the blood flow velocity diagram after use;

[0050] Figure 5a This is the structural diagram and size diagram of the Vitality Coffee 50C;

[0051] Figure 5b This is the action mechanism diagram of Vitality Coffee 50C;

[0052] Figure 6 This is a test chart for DPPH removal ability;

[0053] Figure 7 This is the molecular weight distribution diagram of Starfact HCS. DETAILED DESCRIPTION

[0054] The present invention is further described below with reference to the examples. The test materials used in the present invention are all common commercial products and can be purchased on the market. Among them, Supferm COF and Starfact HCS are prepared by Zhejiang Chenhai Life Sciences Co., Ltd. and supplied by Guangzhou Shengkaisi Chemical Technology Co., Ltd.

[0055] Example 1

[0056] The cosmetics were prepared according to the formula in Table 1, which is a type of anti-hair loss and hair growth water cream in the water cream formula.

[0057] Table 1

[0058]

[0059]

[0060]

[0061] In this embodiment, the preparation method of Vitality Coffee 50C is as follows: dissolving synthetic caffeine in an ethanol solution, adding β-cyclodextrin, stirring for 48 hours, evaporating to complete dryness under vacuum at a temperature of about 50°C, grinding into powder, homogenizing, and sieving to obtain the target product. The mass ratio of the addition amount of synthetic caffeine to cyclodextrin is 1:1.

[0062] In this embodiment, Supferm COFE is composed of yeast fermentation product, yeast fermentation product filtrate, Arabica coffee seed extract, 1,3-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone; wherein the mass fraction of the yeast fermentation product is 57%, the mass fraction of the yeast fermentation product filtrate is 35%, the mass fraction of Arabica coffee seed extract is 1.0%, the mass fraction of 1,3-propylene glycol is 6.0%, the mass fraction of 1,2-hexanediol is 0.5% and the mass fraction of p-hydroxyacetophenone is 0.5%; the Supferm COFE is composed of yeast fermentation product, yeast fermentation product filtrate, Arabica coffee seed extract, 1,3-propylene glycol, 35%, 1,2-hexanediol and p-hydroxyacetophenone; wherein the mass fraction of the yeast fermentation product is 57%, the mass fraction of the yeast fermentation product filtrate is 35%, the mass fraction of the Arabica coffee seed extract is 1.0%, the mass fraction of 1,3-propylene glycol is 6.0%, the mass fraction of 1,2-hexanediol is 0.5% and the mass fraction of p-hydroxyacetophenone is 0.5%. The preparation method of COFE is as follows: prepare fermentation bacteria yeast (purchased from China General Microbiological Culture Collection Management Center, CGMCC2.1543, named: brewer's yeast) and culture medium raw material green coffee beans, grind the coffee beans into powder, and prepare an aqueous solution with water, wherein the mass ratio of coffee powder to water is 1:100, sterilize at 100°C for 30 minutes, access the bacterial liquid, the yeast liquid addition amount is 3%, and aerobic fermentation (rotation speed is 180rpm / min) is carried out for 50 hours, and the fermented liquid is centrifuged at 4500rpm for 30 minutes, sterilized: sterilized at 100°C for 30 minutes, impurities are removed, and 1,3-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone are added to the filtrate at 75°C, and fine filtration is performed to obtain COFE.

[0063] The preparation method of the anti-hair loss and hair growth scalp care water cream containing Supferm COFE and Vitality Coffee 50C is as follows:

[0064] Step 1: After wetting vinyl dimethicone / methicone silsesquioxane crosspolymer and cyclopentasiloxane, add dimethicone PEG-10 / 15 crosspolymer / dimethicone, PEG-9 polydimethylsiloxyethyl dimethicone, dimethicone (and) dimethicone / vinyl dimethicone crosspolymer and dimethicone and isohexadecane components, and homogenize at a high speed with a homogenizing force of 8000 r / min and a homogenizing time of 5 min to obtain component A;

[0065] Step 2: Stir p-hydroxyacetophenone, 1,2-hexanediol and 1,3-butanediol and heat to 70-80°C until transparent, add amino acid moisturizer, trehalose, glycerol, sodium chloride, sodium citrate and water and stir at 600 r / min for 1 minute until uniform, to obtain component B;

[0066] Step 3: Add Vitality Coffee 50C and water in a mass ratio of 1:15, heat to 70-80°C to dissolve, add to component B and stir at 500-700r / min for 1 minute, cool to below 45°C (key step), then add Supferm COFE and Starfact HCS and stir at 500-700r / min for 1 minute until uniform, to obtain component C;

[0067] Step 4: Slowly add C to A while stirring. After adding C, homogenize at a low speed of 2000r / min for about 2min, and then stir at 700r / min for 10min until the paste has a thick texture to obtain the target product.

[0068] The Vitality Coffee 50C made by the cyclodextrin encapsulation technology in this embodiment has the following advantages: Vitality Coffee 50C is an active substance that encapsulates 50% of caffeine by mass in cyclodextrin, and has the following advantages;

[0069] 1. Solubility is improved, up to 10%;

[0070] 2. The active ingredient is encapsulated in cyclodextrin, a nano-scale carrier <1nm, which will not penetrate into the skin but can attach to it, slowly releasing the active ingredient on the skin surface and protecting the active ingredient molecules. Since it can be slowly released on the skin for a long time, its bioavailability is improved and the effect is better than that of free caffeine.

[0071] 3. No cytotoxicity. Allowable usage in the formulation: Higher. In vitro cytotoxicity test: The reagent concentration is 1% and tested in human endothelial cells Caco-2.

[0072] Test results: Human skin irritation <10%, non-irritating (20 patch tests).

[0073] from Figure 2 It can be seen that the encapsulated Vitality Coffee 50C still maintains a high cell survival rate at high concentration (10mM), which shows that it is less toxic to cells than ordinary caffeine.

[0074] Example 2

[0075] A cosmetic was prepared according to the formula in Table 2, which was a control version of the anti-hair loss and hair growth water cream in the water cream formula, without adding Vitality Coffee 50C and Skin Refreshing Coffee Supferm COFE, and the difference with Example 1 was observed when compared.

[0076] Table 2

[0077]

[0078]

[0079] Step 1: After wetting vinyl dimethicone / methicone silsesquioxane crosspolymer and cyclopentasiloxane, add dimethicone PEG-10 / 15 crosspolymer / dimethicone, PEG-9 polydimethylsiloxyethyl dimethicone, dimethicone (and) dimethicone / vinyl dimethicone crosspolymer and dimethicone and isohexadecane components, and homogenize at a high speed with a homogenizing force of 8000 r / min and a homogenizing time of 5 min to obtain component A;

[0080] Step 2: Stir p-hydroxyacetophenone, 1,2-hexanediol and 1,3-butanediol and heat to 70-80°C until transparent, add amino acid moisturizer, Starfact HCS, trehalose, glycerol, sodium chloride, sodium citrate and water and stir at 600 r / min for 1 minute until uniform to obtain component B;

[0081] Step 3: Slowly add B into A while stirring. After adding B, homogenize at a low speed of 2000r / min for about 2min, and then stir at 700r / min for 10min until the paste becomes thick. The target product can be obtained.

[0082] Example 3

[0083] Example 3

[0084] The cosmetics were prepared according to the formula in Table 3, which is a type of anti-hair loss and hair growth water cream in the water cream formula.

[0085] Table 3

[0086]

[0087]

[0088] The preparation method of the anti-hair loss and hair growth scalp care water cream containing Supferm COFE and Vitality Coffee 50C is as follows:

[0089] Step 1: After wetting vinyl dimethicone / methicone silsesquioxane crosspolymer and cyclopentasiloxane, add dimethicone PEG-10 / 15 crosspolymer / dimethicone, PEG-9 polydimethylsiloxyethyl dimethicone, dimethicone (and) dimethicone / vinyl dimethicone crosspolymer and dimethicone and isohexadecane components, and homogenize at a high speed with a homogenizing force of 8000 r / min and a homogenizing time of 5 min to obtain component A;

[0090] Step 2: Stir p-hydroxyacetophenone, 1,2-hexanediol and 1,3-butanediol and heat to 70-80°C until transparent, add amino acid moisturizer, Starfact HCS, trehalose, glycerol, sodium chloride, sodium citrate and water and stir at 600 r / min for 1 minute until uniform to obtain component B;

[0091] Step 3: Add Vitality Coffee 50C and water in a mass ratio of 1:15, heat to 70-80°C to dissolve, add to component B and stir at 500-700r / min for 1 minute, cool to below 45°C, then add Supferm COFE and Starfact HCS and stir at 500-700r / min for 1 minute until uniform, to obtain component C;

[0092] Step 4: Slowly add C to A while stirring. After adding C, homogenize at a low speed of 2000r / min for about 2min, and then stir at 700r / min for 10min until the paste has a thick texture to obtain the target product.

[0093] Example 4

[0094] In order to demonstrate the effect of the skin-awakening coffee Supferm COFE prepared by Zhejiang Chenhai Life Sciences Co., Ltd. in the present invention, Examples 1, 2, and 3 are renumbered according to the amount of skin-awakening coffee Supferm COFE added, with the amount of skin-awakening coffee Supferm COFE added being 0% being recorded as sample #1, the amount of skin-awakening coffee Supferm COFE added being 5% being recorded as sample #2, and the amount of skin-awakening coffee Supferm COFE added being 30% being recorded as sample #3. Two product application concentrations of 10% and 50% were tested, and the results are as follows:

[0095] Test Concentration | Sample No. 1 2 3 50％ -24.30％ 48.05％ 93.93％ 10％ -9.97％ 7.17％ 54.83％

[0096] from Figure 6 It can be seen that the test value of sample 1# without Supferm COFE is negative, which can be considered to have no DPPH scavenging ability. Samples 2# and 3# both have good DPPH scavenging ability. When the amount of Supferm COFE added is 30% and the test concentration is 50%, the DPPH scavenging ability is as high as 93.93%.

[0097] 1. Testing of physical and chemical properties.

[0098] a) Apply the anti-hair loss water cream obtained in Example 1 and the blank water cream obtained in Example 2 to the skin and massage gently, and water droplets can be seen; b) Stability test under external force: The water cream formula is centrifuged at a speed of 2000r / min for 30min, and the water-oil phase separation of the water cream formula is observed. The results show that no phase separation is observed in the two water cream formulas; c) Heat resistance, cold resistance, and cycle experiment: The water cream formula is placed in an environment of 48℃ heat resistance for 90 days, -18℃ cold resistance for 90 days, and 24h 48℃, 24h room temperature, 24h -18℃ (a total of 3 cycles) cycle, and the water and oil phase separation of the water cream formula is observed. The results show that in the environment of 90 days of cold and hot and 3 cycles of cold and hot cycles, the water cream formula does not undergo phase separation. The specific physical and chemical basic properties are shown in Tables 4 and 5.

[0099] Table 4 Basic physical and chemical properties of anti-hair loss scalp care water cream containing Supferm COFE and Vitality Coffee 50C in Example 1

[0100]

[0101] Table 5 Basic physical and chemical properties of the comparative version of the anti-hair loss scalp care water cream of Example 2

[0102]

[0103] Example 5

[0104] The product obtained in Example 1 was used to apply it to the alopecia areata area after washing the hair every day. New hair grew out after two months, and then the hair was normal and long all the time. Figure 3a As shown, the testing dates are from October 17, 2021 to December 17, 2021.

[0105] Use the product obtained in Example 1 and apply it to the sparse hair on the forehead after washing your hair every day. After one week, some hair will grow. Figure 3b and Figure 3c As shown, the test date is November 25, 2022 ( Figure 3b ) to December 2, 2022 ( Figure 3c ).

[0106] Example 6

[0107] Proof of Vitality Coffee 50C in promoting blood circulation:

[0108] Instrument used: X-CRYSTAL (ray crystal analyzer)

[0109] Test principle: By observing the capillaries at the fingernail wrinkles

[0110] Test process: Before application, use X-CRYSTAL to test the blood flow of fingers. Apply 5% Vitality Coffee 50C basic lotion on the fingers of volunteers, wait for 5-10 minutes for the lotion to be completely absorbed, and then test the blood flow again.

[0111] Test method: observe blood flow velocity, the results are as follows Figure 4a and Figure 4b ,in Figure 4a This is the picture before use. Figure 4b This is the picture after use. Figure 4a and Figure 4b It can be observed that after applying 5% Vitality Coffee 50C basic lotion, the blood flow rate is significantly accelerated.

Claims

1. A hair loss prevention and hair growth cream containing coffee bean fermentation liquid Supferm COFE and vitality coffee, characterized in that: In terms of mass percentage, it includes 1%~3% emulsifier, 6%~12% conditioning agent, 0.5%~2% preservative, 10%~15% moisturizer, 5%~35% Supferm COFE, 0.5~5% Starfact HCS and 1%~3% Vitality Coffee 50C, and the rest is deionized water; The Supferm COFE skin-awakening coffee is composed of yeast fermentation product, yeast fermentation product filtrate, Arabica coffee seed extract, 1,3-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone; wherein the mass fraction of the yeast fermentation product is 57%, the mass fraction of the yeast fermentation product filtrate is 35%, the mass fraction of Arabica coffee seed extract is 1.0%, the mass fraction of 1,3-propylene glycol is 6.0%, the mass fraction of 1,2-hexanediol is 0.5% and the mass fraction of p-hydroxyacetophenone is 0.5%; the Supferm COFE skin-awakening coffee is composed of yeast fermentation product, yeast fermentation product filtrate, Arabica coffee seed extract, 1,3-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone. The preparation method of COFE is as follows: prepare fermentation bacteria yeast and culture medium raw material green coffee beans, grind the coffee beans into powder, and prepare an aqueous solution with water, wherein the mass ratio of coffee powder to water is 1:100, sterilize at 100°C for 30 minutes, then inoculate with bacterial liquid, add 3% yeast liquid, aerobically ferment at a speed of 180rpm for 50 hours, centrifuge the fermented liquid at 4500rpm for 30 minutes, sterilize at 100°C for 30 minutes, remove impurities, take the filtrate and add 1,3-propylene glycol, 1,2-hexanediol and p-hydroxyacetophenone at 75°C, and finely filter to obtain; the yeast is Saccharomyces cerevisiae, purchased from China General Microbiological Culture Collection Management Center, numbered CGMCC2.1543; The preparation process of Vitality Coffee 50C is as follows: dissolving synthetic caffeine in an ethanol solution, adding β-cyclodextrin, stirring for 48 hours, evaporating to complete dryness under vacuum at a temperature of about 50°C, grinding into powder, homogenizing, and sieving to obtain the target product; the mass ratio of the synthetic caffeine to β-cyclodextrin is 1:1; The Starfact HCS is composed of hydrolyzed collagen PG-propylmethylsilanediol and water, wherein the mass percentage of hydrolyzed collagen PG-propylmethylsilanediol is 10%, and the remaining 90% is water.

2. The anti-hair loss and hair growth cream containing coffee bean fermentation liquid Supferm COFE and vitality coffee according to claim 1, characterized in that: The Vitality Coffee 50C comprises an inclusion and a coating, wherein the inclusion is caffeine, and the coating is a cylindrical nanomaterial cyclodextrin, and the inner cavity diameter of the coating is less than 1 nm.

3. The anti-hair loss and hair growth cream containing coffee bean fermentation liquid Supferm COFE and vitality coffee according to claim 1, characterized in that: The emulsifier includes one or more of dimethicone PEG-10 / 15 crosspolymer / dimethicone and PEG-9 polydimethylsiloxyethyl dimethicone.

4. The anti-hair loss and hair growth cream containing coffee bean fermentation liquid Supferm COFE and vitality coffee according to claim 1, characterized in that: The conditioning agent includes at least one of isohexadecane, cyclopentasiloxane, polydimethylsiloxane, vinyl polydimethylsiloxane / polymethylsiloxane silsesquioxane cross-linked polymer, sodium chloride, and sodium citrate.

5. The anti-hair loss and hair growth cream containing coffee bean fermentation liquid Supferm COFE and vitality coffee according to claim 1, characterized in that: The moisturizing agent is one or more of glycerol, 1,3-butylene glycol, amino acid moisturizing agent, trehalose, and 1,2-hexanediol.

6. The anti-hair loss and hair growth cream containing coffee bean fermentation liquid Supferm COFE and vitality coffee according to claim 1, characterized in that: The preservative is p-hydroxyacetophenone.

7. The method for preparing the anti-hair loss and hair growth cream containing coffee bean fermentation liquid Supferm COFE and vitality coffee as claimed in any one of claims 1 to 6, characterized in that: The steps include: Step 1: Mix the emulsifier and the conditioner and homogenize them at a homogenization speed of 3000-8000 r / min for 3-10 min to obtain component A; Step 2: Stir p-hydroxyacetophenone, 1,2-hexanediol and 1,3-butanediol and heat to 70-80°C until they become transparent, add other moisturizer components and water and stir until uniform to obtain component B; Step 3: Mix Vitality Coffee 50C and water in a mass ratio of 1:20, heat to 70-80°C to dissolve, add to component B and stir, cool to below 45°C, add Supferm COFE and Starfact HCS and stir until uniform, to obtain component C; Step 4: Slowly add component C to component A while stirring. After adding component C, homogenize at a low speed and stir until the paste has a thick texture to obtain the target product.

8. The method for preparing the anti-hair loss and hair growth water cream containing coffee bean fermentation liquid Supferm COFE and vitality coffee according to claim 7, characterized in that: In step 2 and step 3, the stirring is performed at 500-700 r / min for 1-3 minutes.

9. The method for preparing the anti-hair loss and hair growth water cream containing coffee bean fermentation liquid Supferm COFE and vitality coffee according to claim 7, characterized in that: The low-speed homogenization is 2000-3000 r / min for 2-5 minutes; the stirring is 500-700 r / min for 5-10 minutes.

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