Electrophoretic system, electrophoretic device, and electrophoretic analysis method

Abstract

The present application provides an electrophoresis system, an electrophoresis device, and an electrophoresis analysis method. The electrophoresis system has an electrophoresis device, an analysis device, and a display unit. Moreover, the analysis device is configured to switch between a detailed display state and an enlarged display state. The detailed display state is a state in which the result display area of the display unit displays a plurality of analysis result confirmation displays including gel image display. The enlarged display state is a state in which the result display area of the display unit only displays the gel image display in the plurality of analysis result confirmation displays in an enlarged manner.

Description

Technical Field

[0001] This invention relates to an electrophoresis system, an electrophoresis apparatus, and an electrophoresis analysis method. Background Technology

[0002] Previously, an analytical apparatus for obtaining separation data through electrophoretic analysis was known. Such an apparatus is disclosed, for example, in Japanese Patent Application Publication No. 2020-106351.

[0003] The analytical apparatus described in Japanese Patent Application Publication No. 2020-106351 analyzes separation data obtained through electrophoretic analysis using an electrophoresis apparatus. In analyzing the separation data obtained through electrophoretic analysis, the components contained in the target sample are identified by comparing reference data (which is separation data for a reference sample) with target data (which is separation data for the target sample). The reference sample contains known components. The analytical apparatus described in Japanese Patent Application Publication No. 2020-106351 acquires separation data obtained through electrophoretic analysis using an electrophoresis apparatus. Furthermore, the analytical apparatus determines the separation index values ​​of each component peak in the separation data and determines whether the component peaks in the reference data and the target data are identical. Additionally, the analytical apparatus described in Japanese Patent Application Publication No. 2020-106351 displays the gel images of the target data and the reference data in an arranged configuration on a display device, making it visually easy to identify the component peaks present in the target data. The gel image shows a band pattern indicating the separation index values ​​of the component peaks that have been separated by electrophoresis.

[0004] While not explicitly described in Japanese Patent Application Publication No. 2020-106351, generally speaking, when analyzing separation data obtained through electrophoresis, the display device (display unit) shows the positions of the sample wells configured for the sample being measured, along with the waveform of the separation data (measured values) obtained by the electrophoresis apparatus, along with the gel image. However, since the size of the area where the gel image is displayed in the display area of ​​the display device is limited, multiple bands with similar values ​​may overlap in the displayed gel image's band pattern. In this case, even if the displayed gel image is checked, it is difficult to determine whether separation was performed correctly in the electrophoretic analysis. Therefore, it is desirable to improve the visibility of the distribution (band pattern) of components separated by electrophoresis in the gel image display. Summary of the Invention

[0005] The present invention was made to solve the problems described above. One object of the present invention is to provide an electrophoresis system, electrophoresis apparatus, and electrophoresis analysis method that can improve the visibility of the distribution of components separated by electrophoresis in gel image display.

[0006] To achieve the above objectives, the electrophoresis system according to a first aspect of the present invention comprises: an electrophoresis apparatus including a measuring unit that measures a test object separated by electrophoresis in a flow path including a separation flow path for separating test objects; an analysis device that analyzes the components of the test objects separated by electrophoresis based on the measured values ​​of the test objects measured by the measuring unit; and a display unit that displays a plurality of analysis result confirmation displays, including at least a gel image display showing the distribution of components of the test objects analyzed by the analysis device, in a result display area, wherein the analysis device is configured to switch between a detailed display state and a magnified display state, wherein the detailed display state is a state in which the result display area of ​​the display unit displays a plurality of analysis result confirmation displays, including at least a gel image display, and the magnified display state is a state in which the result display area of ​​the display unit magnifies only the gel image display among the plurality of analysis result confirmation displays.

[0007] The electrophoresis apparatus of the second aspect of the present invention includes a measurement unit that measures a test object separated by electrophoresis in a flow path including a separation flow path for separating the test object. The electrophoresis apparatus is configured to switch between a detailed display state and a magnified display state. The detailed display state is a state in which the result display area of ​​the display unit displays at least a plurality of analytical result confirmation displays including a gel image display, wherein the gel image display shows the distribution of components of the test object as resolved based on the measurement value of the test object measured by the measurement unit. The magnified display state is a state in which the result display area of ​​the display unit displays only the gel image display from the plurality of analytical result confirmation displays.

[0008] The electrophoretic analysis method in the third aspect of the present invention includes the following steps: analyzing the components of the separated test objects based on the measured values ​​obtained by measuring the test objects separated by electrophoresis in a flow path including a separation flow path for separating the test objects; and switching between a detailed display state and a magnified display state, wherein the detailed display state is a state in which the result display area of ​​the display unit displays at least a plurality of analysis result confirmation displays including gel image displays, wherein the gel image displays show the distribution of the components of the analyzed test objects, and the magnified display state is a state in which the result display area of ​​the display unit displays only the gel image displays in the plurality of analysis result confirmation displays.

[0009] In the electrophoresis system of the first aspect, the electrophoresis apparatus of the second aspect, and the electrophoretic analysis method of the third aspect described above, switching is performed between a detailed display state and a magnified display state. The detailed display state displays multiple analysis result confirmation displays, including at least the gel image display, in the result display area of ​​the display unit. The magnified display state magnifies only the gel image display among the multiple analysis result confirmation displays in the result display area of ​​the display unit. Therefore, by switching between the detailed display state and the magnified display state, it is possible to switch between a state where multiple analysis result confirmation displays are shown in the result display area of ​​the display unit and a state where only the gel image display is shown in the result display area of ​​the display unit. Thus, when multiple bands with similar values ​​exist in the banded pattern of the gel image display in the detailed display state, switching to the magnified display state can be used to magnify the gel image display. As a result, the visibility of the distribution of components separated by electrophoresis in the gel image display can be improved. Attached Figure Description

[0010] Figure 1 This is a block diagram showing the overall structure of the electrophoresis system of this embodiment.

[0011] Figure 2 This is a schematic diagram illustrating the structure of the electrophoresis apparatus of this embodiment.

[0012] Figure 3 This is a diagram illustrating the structure of a chip with flow paths for electrophoresis.

[0013] Figure 4 This is a diagram showing an example of a measurement value obtained through measurement by the measuring unit.

[0014] Figure 5 This is a diagram showing an example of the display section in detailed display mode.

[0015] Figure 6 This diagram shows an example of the display unit in magnified display mode.

[0016] Figure 7 This is a flowchart illustrating an electrophoretic analysis method for one implementation. Detailed Implementation

[0017] The following describes one embodiment of the invention based on the accompanying drawings.

[0018] (Overall structure of the electrophoresis system)

[0019] Reference Figures 1-6 An electrophoresis system 100 according to one embodiment of the present invention will be described.

[0020] like Figure 1 As shown, the electrophoresis system 100 of this embodiment includes an electrophoresis apparatus 101 and an analytical apparatus 102.

[0021] The electrophoresis apparatus 101 separates the analyte by electrophoresis using three chips 60a, 60b, and 60c, thereby determining the components contained in the analyte. Specifically, in the electrophoresis apparatus 101, a sample preparation section 71 (see reference 60a) is pre-positioned on the plate 70 and the sample preparation section 71. Figure 2 The measurement targets are located in flow paths 61 (refer to) respectively set in chips 60a to 60c. Figure 3 The components are separated by electrophoresis. Then, the electrophoresis apparatus 101 measures the degree of separation (the extent of distribution of each component) of the test objects separated by electrophoresis.

[0022] <Structure of the electrophoresis apparatus>

[0023] like Figure 1 and Figure 2 As shown, the electrophoresis apparatus 101 includes a supply unit 10, a voltage application unit 20, a measurement unit 30, and a control unit 40.

[0024] In the electrophoresis apparatus 101, the analyte and separation buffer are supplied to the respective flow paths 61 of chips 60a, 60b and 60c by the operation of the supply unit 10 for electrophoresis-based measurement.

[0025] The analyte may include, for example, DNA (deoxyribonucleic acid), RNA (ribonucleic acid), or protein. The analyte includes the analyte sample (sample) for which the separation degree of each component obtained by electrophoresis is to be determined, and a reference sample (size standard) that serves as a benchmark for the electrophoresis-based determination of the analyte sample. The analyte sample is the measured value 111 (refer to) measured using the measuring unit 30. Figure 4 The target sample (subject to electrophoresis) is the analyte for which the separation degree is unknown. Conversely, the reference sample is the analyte containing nucleic acids or proteins whose separation characteristics, such as molecular weight (chain length), are already known. In other words, the target sample is the analyte with unknown composition, while the reference sample is the analyte with known composition.

[0026] Furthermore, the test objects are arranged on the plate 70 and the sample placement section 71. The plate 70 has multiple wells 70a as placement positions for the test objects. For example, the plate 70 has 96 wells 70a arranged in an 8×12 grid pattern. The plate 70 is arranged by the operator in the plate placement positions inside the electrophoresis apparatus 101, with all or part of each well 70a containing multiple types of test objects. Then, the test objects are placed in the sample placement section 71 independently of the plate 70. Additionally, the test...

[0027] The sample configuration section 71 has sample holes 71a at multiple configuration positions for configuring the measurement object. The sample holes 71a are configured in a 3×12 grid pattern.

[0028] The separation buffer is filled into the flow path 61 of chips 60a, 60b and 60c respectively (see reference) before being supplied to the analyte. Figure 3 The separation medium. The separation buffer, for example, contains at least one of a pH buffer material and a water-soluble polymer (such as a cellulose-based polymer). Additionally, the separation buffer is filled into a container not shown.

[0029] The buffer container. Alternatively, the separation buffer can be placed in the plate 70 or the sample preparation section 71. In the electrophoresis apparatus 101, the analyte is supplied with the separation buffer pre-filled inside the flow path 61 for electrophoresis.

[0030] like Figure 2 As shown, the supply unit 10 includes a probe 11 and a pump 12. The supply unit 10 supplies the separation buffer and the test object (test object sample) disposed on the plate 70 or the sample placement section 71 by moving the probe 11.

[0031] (And reference samples) are supplied to chips 60a-60c. Pump 12 adjusts the pressure of probe 11 for the aspiration and ejection of separation buffer and 5 analytes.

[0032] like Figure 3 As shown, each of chips 60a to 60c has a flow path 61 inside. Chips 60a, 60b, and 60c have identical structures. In the following description, details of chip 60a are illustrated and explained, while descriptions of chips 60b and 60c are omitted as they are identical to those of chip 60a.

[0033] Chip 60a is an electrophoresis microchip with a flow path 61 for electrophoresis disposed inside a pair of combined planar components. Flow path 61 includes a separation flow path 62 and a preparation flow path 63. The separation flow path 62 and the preparation flow path 63 are arranged to intersect each other. The separation flow path 62 is provided for separating the analyte by electrophoresis. In addition, the preparation flow path 63 is provided for guiding the analyte to the separation flow path 62.

[0034] Furthermore, reservoirs 64a and 64b are provided at both ends of the preparation flow path 63, serving as spaces for supplying and aspirating the separation buffer and the analyte. Similarly, reservoirs 64c and 64d are provided at both ends of the separation flow path 62. Electrodes 65a and 65b are respectively disposed in the reservoirs 64a and 64b at both ends of the preparation flow path 63. Electrodes 65c and 65d are respectively disposed in the reservoirs 64c and 64d at both ends of the separation flow path 62.

[0035] In the electrophoresis apparatus 101, electrophoresis is performed by applying voltage from the voltage application unit 20 to a plurality of electrodes 65a to 65d disposed in the flow path 61. Furthermore, the magnitude of the voltage applied to the electrodes 65a to 65d is controlled by the control unit 40. In addition, three voltage application units 20 are provided, each corresponding to a chip 60a to 60c (see reference). Figure 2 The voltage application unit 20 applies a current voltage to the current path 61 of each of the chips 60a to 60c. That is, for chips 60b and 60c, a DC voltage is applied to the current path 61 using the voltage application unit 20 in the same way as for chip 60a.

[0036] In the electrophoresis apparatus 101, when performing electrophoresis-based measurements on the chip 60a, firstly, the supply unit 10 fills the entire interior of the flow path 61 (separation flow path 62 and preparation flow path 63) with separation buffer. Then, for example, the supply unit 10 supplies the sample material drawn from the designated well 70a of the plate 70 to the reservoir 64a of the preparation flow path 63. Next, by applying a predetermined voltage to the electrodes 65a to 65d using the voltage application unit 20, the sample material moves within the preparation flow path 63 until it intersects with the separation flow path 62. Then, by varying the voltage applied from the voltage application unit 20 to each of the electrodes 65a to 65d, the sample material moves towards the electrode 65d (reservoir 64d) within the separation flow path 62 while being separated by electrophoresis.

[0037] At this time, in the electrophoretic separation measurement, the analyte moves within the separation flow path 62 at different speeds depending on the molecular weight (chain length) and other separation characteristics of its constituent components. In the electrophoresis apparatus 101, the separation characteristics of each component of the analyte are measured by sequentially measuring the components arriving at the measurement positions 66 within the separation flow path 62. Thus, in the electrophoresis apparatus 101, the components contained in the analyte are measured according to the degree of separation (migratory power).

[0038] like Figure 2As shown, the measurement unit 30 measures the test objects that have been separated by electrophoresis in the flow paths 61 of each of the multiple (3) chips 60a to 60c. For example, the measurement unit 30 performs fluorescence detection on the components of the test objects separated by electrophoresis. The measurement unit 30 has a measurement position 66 (see reference) in the separation flow path 62. Figure 3 An LED 31 (light-emitting diode) is used to irradiate the sample with excitation light. Furthermore, the excitation light from the LED 31 is irradiated onto each component of the sample as it moves through the separation flow path 62 while being separated by electrophoresis, thereby exciting each component of the sample to emit fluorescence. The measurement unit 30 measures this fluorescence using a photomultiplier tube 32, for example, via an optical fiber and a filter component, thereby measuring the components of the sample that have been separated by electrophoresis.

[0039] like Figure 4 As shown, the photomultiplier tube 32 outputs a measurement signal representing the measurement value 111 to the control unit 40 based on the detected fluorescence intensity. In the measurement value 111 measured by the measurement unit 30, the measurement object, which moves while being separated by electrophoresis, passes the measurement position 66 (see reference). Figure 3 The time of peaks indicates the largest values ​​(peaks). Therefore, based on the size and position (time) of the peaks of each component contained in the analyte, the degree of distribution of each component contained in the analyte is used to analyze the components (concentration) and composition (size).

[0040] Furthermore, the electrophoresis apparatus 101 is equipped with a cleaning mechanism (not shown). The electrophoresis apparatus 101 is cleaned each time a measurement of a analyte is performed in each part, including chips 60a-60c and the supply unit 10. Moreover, the electrophoresis apparatus 101 is configured to use the cleaning mechanism to clean residual analytes and separation buffer in the flow path 61, thereby repeatedly performing measurements on each chip in chips 60a-60c. In this way, the electrophoresis apparatus 101 sequentially performs measurements on each of the multiple analytes arranged in multiple sample wells 70a and 71a.

[0041] The control unit 40 controls the operation of each part of the electrophoresis apparatus 101. The control unit 40 is, for example, a microcomputer (microcontroller) equipped with a processing unit such as a CPU (Central Processing Unit) and a storage device such as flash memory. Furthermore, the control unit 40 includes a communication module, configured to communicate with the analysis device 102. Moreover, based on drive signals from the analysis device 102, the control unit 40 controls the operation of each part of the electrophoresis apparatus 101 in a manner that sequentially performs electrophoretic measurements on multiple test objects arranged on the plate 70 and the sample placement unit 71.

[0042] Specifically, the control unit 40 operates the supply unit 10 based on a drive signal from the analysis device 102, thereby sequentially supplying the test objects arranged in the sample wells 70a of the plate 70 in a manner that, for example, one type of measurement is performed on each of the chips 60a to 60c. Then, the control unit 40 separates (moves) the test objects by applying voltage to the flow paths 61 of each of the chips 60a to 60c using the voltage application unit 20. Furthermore, the control unit 40 acquires the measurement values ​​111 measured by the measurement unit 30, which is arranged to correspond to each of the chips 60a to 60c. Then, the control unit 40 acquires the measurement values ​​111 for each of the sample wells 70a of the plate 70 and for each of the sample wells 71a of the sample placement unit 71. Finally, the control unit 40 outputs the measurement values ​​111 of the test objects measured by the measurement unit 30 to the analysis device 102 for each of the chips 60a to 60c.

[0043] <Structure of the analytical apparatus>

[0044] like Figure 1 As shown, the analysis device 102 includes an operation unit 51, a display unit 52, a storage unit 53, and a control unit 54. The analysis device 102 is a computer used to analyze the components of a test object separated by electrophoresis based on the measurement value 111 of the test object measured by the electrophoresis apparatus 101. The analysis device 102 is configured to communicate with the electrophoresis apparatus 101 and to acquire the measurement value 111 acquired by the electrophoresis apparatus 101.

[0045] The operation unit 51 accepts input operations from the operator. Furthermore, the operation unit 51 outputs an operation signal based on the accepted input operation to the control unit 54. The operation unit 51 may be a pointing device such as a keyboard or mouse.

[0046] The display unit 52 is, for example, a monitor such as a liquid crystal display. Furthermore, the display unit 52 displays the input information according to the control of the control unit 54. Additionally, the display unit 52 displays the analysis results of the measurement object by the control unit 54 of the analysis device 102. In this embodiment, the display unit 52 displays multiple analysis results confirmation displays in the result display area 52a (see reference). Figure 5 and Figure 6 Details of the display on display unit 52 will be described later.

[0047] The storage unit 53 is composed of a storage device such as a hard disk drive or an SSD (Solid State Drive). The storage unit 53 stores the measurement values ​​111 obtained using the electrophoresis apparatus 101. Additionally, the storage unit 53 stores an electrophoresis analysis program 53a for operating the control unit 54. Furthermore, the storage unit 53 stores various parameters, including preset settings or settings (measurement conditions) input by the operator.

[0048] The control unit 54 is a computer including a CPU, RAM (Random Access Memory), and ROM (Read Only Memory). The control unit 54 executes the program (electrophoresis analysis program 53a) stored in the storage unit 53 to control each part of the analysis device 102. Furthermore, the control unit 54 is configured to communicate with the control unit 40 of the electrophoresis apparatus 101 via a communication module (not shown).

[0049] (Details of the control measures performed by the analytical device)

[0050] The control unit 54 sends a drive signal to the control unit 40 to operate the electrophoresis apparatus 101. Specifically, based on the input operation received by the operation unit 51, the control unit 54 acquires various parameters for performing electrophoresis. For example, based on the input operation to the operation unit 51, the control unit 54 acquires sample well information indicating the sample wells 70a and 71a on which the test objects (reference sample and test object sample) to be measured are arranged, measurement condition information including information on the magnitude and time of the applied voltage, and arrangement table information indicating the measurement order of the test objects arranged in the multiple sample wells 70a and 71a. In addition, the sample well information, measurement condition information, and arrangement table information can also be selected from a database pre-stored in the storage unit 53. Then, the control unit 54 sends a drive signal containing the acquired sample well information, measurement condition information, and arrangement table information to the control unit 40 of the electrophoresis apparatus 101. Then, the control unit 54 acquires the measurement value 111 obtained by the control unit 40 based on the control performed by the control unit 40 on the sent drive signal.

[0051] Then, as Figure 5 and Figure 6As shown, the control unit 54 analyzes the analyte separated by electrophoresis based on the acquired measurement value 111. Then, the control unit 54 displays the analysis results of the analyte in the result display area 52a of the display unit 52. Specifically, the control unit 54 analyzes the size (separation index value) of each component of the analyte separated by electrophoresis based on the acquired measurement value 111. For example, in the case of DNA, the analyzed size is represented by the DNA strand length (number of base pairs).

[0052] <Display switching>

[0053] Furthermore, in this embodiment, the analysis device 102 (control unit 54) is configured to switch between a detailed display state and a magnified display state based on an operation received by the operation unit 51. Specifically, the analysis device 102 displays the switch button display 52b selectively on the upper side of the display unit 52. Moreover, the analysis device 102 is configured to switch the display of the analysis results of the measured object in the result display area 52a of the display unit 52 between a detailed display state and a magnified display state based on a selection operation such as a click operation on the switch button display 52b received by the operation unit 51.

[0054] <Detailed display of status information>

[0055] like Figure 5 As shown, in the detailed display state, the analysis device 102 (control unit 54) is configured to display multiple analysis result confirmation displays, including the sample well position display 91, the measurement waveform display 92, the peak table 93, and the gel image display 94, in the result display area 52a of the display unit 52.

[0056] The well position display 91 shows the position of each well among the multiple wells 70a and 71a configured for each of the multiple test objects. In the well position display 91, the positions of each well among the multiple wells 70a and 71a are shown in a grid pattern corresponding to the arrangement of the wells 70a and 71a in a grid pattern. For example, the positions of 96 wells 70a of 8×12 are displayed as a grid-like quadrilateral (rectangle) arranged in 8 columns (A to H) and 1 to 12 columns (X1 to X3). Similarly, the positions of wells 71a of 3×12 are displayed as a grid-like quadrilateral (rectangle) arranged in 8 columns (A to H) and 3 columns (X1 to X3).

[0057] Furthermore, in the sample hole position display 91, the sample holes 70a or 71a where the test objects are located are indicated by circles displayed inside a grid-like quadrilateral. Additionally, the display of two overlapping circles indicates that multiple measurements are performed on the test objects located in the same sample hole 70a or 71a. Moreover, how the test objects (reference sample and test object sample) are arranged in the multiple sample holes 70a and 71a is set based on input operations to the operation unit 51 or data stored in the storage unit 53.

[0058] In addition, such as Figure 5 As shown, the measurement waveform display 92 is a waveform (electrophoresis diagram) showing the time series values ​​of the acquired measurement values ​​111. Specifically, the measurement waveform display 92 is represented by the signal intensity (measurement value 111) of the measurement unit 30, with the horizontal axis as the dimension and the vertical axis as the measurement value of the time series values ​​of the acquired measurement values ​​111. The measurement waveform display 92 also displays numerical values ​​representing the dimensions of the separated components of the measurement object.

[0059] Here, an internal standard marker, which serves as a reference for analyzing the composition of the analytes, is mixed into the analytes separated by electrophoresis. That is, in electrophoresis-based measurements, the internal standard marker, which serves as a reference for the minimum and maximum values ​​of the size (chain length) measured by electrophoresis, is supplied to the flow path 61 along with the analytes. Specifically, the internal standard marker is disposed in each of the sample wells 70a and 71a in a mixed state with each analyte. The internal standard marker has a lower marker (hereinafter referred to as LM) and an upper marker (hereinafter referred to as UM). LM is measured in the measurement unit 30 with a size sufficiently small compared to the analytes. Furthermore, UM is measured in the measurement unit 30 with a size sufficiently large compared to the analytes. In other words, LM is a size sufficiently small compared to both the reference sample and the analyte sample, and UM is a size sufficiently large compared to both the reference sample and the analyte sample.

[0060] In the electrophoresis-based measurement, the LM and UM of both the reference sample and the test sample are mixed. Then, based on the LM and UM measured when the reference sample was measured and the LM and UM measured when the test sample was measured, the time when the measured value 111 of the reference sample and the measured value 111 of the test sample were measured are compared, thereby resolving the size of the test sample. Furthermore, which of the multiple test samples arranged in the sample wells 70a and 71a is the reference sample is predetermined. In addition, when multiple reference samples are arranged in the sample wells 70a or 71a, the reference sample on which the test sample is analyzed is predetermined for each test sample.

[0061] In detail, firstly, an electrophoretic measurement of a reference sample, which serves as the standard for measurement, is performed. Then, the analytical apparatus 102 detects peaks from the waveform of the measured value 111 of the reference sample. Then, based on the detected LM, UM, and peaks of each component, the size of LM is set to 0, the size of UM is set to a predetermined value, and the ratio of the time (moment) at which the measuring unit 30 detects the lower limit marker (LM) and the upper limit marker (UM) to the time (moment) at which the measuring unit 30 detects each component (peak) contained in a reference sample of a predetermined size is obtained, thereby generating a calibration curve.

[0062] Then, as Figure 5 As shown, the analysis device 102 (control unit 54) analyzes the size of the test sample based on the measured value 111 obtained by electrophoresis in a state where the test sample, which is a test object with unknown size, is mixed with LM and UM, and the generated calibration curve. Specifically, the analysis device 102 detects peaks from the waveform generated based on the measured value 111 obtained by measuring the test sample with unknown size. Then, the analysis device 102 detects LM and UM from the detected peaks, and analyzes the size corresponding to the peak based on the relative time ratio (movement time index) of the peaks contained between LM and UM, and according to the calibration curve of the reference sample. Then, the analysis device 102 displays the size corresponding to each detected peak in the measured waveform display 92. In addition, the analysis device 102 displays the specific values ​​of the size corresponding to the detected peak and the movement time index in the peak table 93.

[0063] The gel image display 94 shows the resolution results (sizes) of each of the multiple measurement objects. Specifically, in the gel image display 94, for each measurement of the measurement object using chips 60a to 60c, a display (image) showing the distribution of the components (sizes) of each of the multiple measurement objects resolved by the resolution device 102 is arranged as multiple resolution results. In the resolution results in the gel image display 94, the size of each resolved component of the measurement object is represented by multiple horizontal lines (band patterns, trapezoids). In addition, in the resolution results in the gel image display 94, LM is set as the lower end and UM is set as the upper end, and multiple horizontal lines are arranged at positions corresponding to the peaks of the waveform of the measured value 111 according to the size. Furthermore, in the gel image display 94, it is shown that in the multiple resolution results arranged in the array, the positions showing LM and the positions showing UM are common positions. For example, in the resolution results of the gel image display 94, the pixel value is set according to the magnitude of the measured value 111 (signal strength), thereby representing the magnitude of the measured value 111 by the intensity of the color.

[0064] Furthermore, the gel image display 94 shows the well numbers of the sample wells 70a or 71a containing the test objects corresponding to each analysis result, as well as the test sequence number indicating the measurement order. Additionally, the analysis device 102 is configured to display chip numbers in the gel image display 94. The chip numbers are displayed as numbers 1 to 3 for each of the chips 60a to 60c. Moreover, in the gel image display 94, during the analysis of the measurement values ​​111 obtained by measuring each chip in chips 60a to 60c, the chip numbers are displayed only for the analysis results of the reference sample, which serves as the reference for generating the calibration curve.

[0065] Furthermore, the analysis device 102 (control unit 54) is configured to display a measurement waveform display 92 and a peak table 93 corresponding to one of the multiple analysis results selected from the gel image display 94 on the display unit 52. Specifically, the operation unit 51 receives a selection operation to select one analysis result from the images showing multiple analysis results arranged in the gel image display 94. The analysis device 102 is configured to display the measurement waveform display 92 and the peak table 93 corresponding to the selected analysis result on the display unit 52 based on the selection operation received by the operation unit 51. In addition, a frame-shaped identification display is displayed on the gel image display 94 to enable identification of the selected analysis result. Furthermore, textual information such as the position of the sample wells 70a and 71a corresponding to the selected analysis result, the type of the test object (reference sample or test object sample), and the number of the chips 60a to 60c used for the measurement can also be displayed on the display unit 52.

[0066] Furthermore, in this embodiment, the analysis device 102 (control unit 54) displays a scale 94a on the gel image display 94. The scale 94a shows the separation scale of the separated components of the test object in the analysis results displayed on the gel image display 94. Specifically, the scale 94a represents the size of each component of the test object that has been analyzed. Additionally, a numerical value corresponding to the scale 94a is displayed on the gel image display 94. The numerical value of the scale 94a represents a specific numerical value of the size of each component of the test object. Furthermore, the numerical value of the scale 94a is obtained based on the analysis of the components of the reference sample in the test object. That is, the size of the components contained in the reference sample (the size of the detected peak) set for generating the calibration curve is obtained as the numerical value corresponding to the scale 94a.

[0067] Furthermore, in this embodiment, the analysis device 102 (control unit 54) is configured to reduce and periodically reject the number of values ​​displayed in the gel image display 94 corresponding to the scale 94a, so that the values ​​of the scale 94a do not overlap. Additionally, the analysis device 102 periodically rejects the number of displayed values ​​while suppressing unevenness in the display intervals of the values ​​of the scale 94a. Specifically, when the size of the reference sample is displayed on the scale 94a of the gel image display 94 and the values ​​overlap, the analysis device 102 periodically rejects the number of displayed values ​​of the scale 94a. At this time, among the values ​​corresponding to the scale 94a, the values ​​to be periodically rejected are set such that the intervals between adjacent displayed values ​​are approximately equal in the vertical direction (the difference in the vertical direction does not increase).

[0068] Furthermore, in this embodiment, the analysis device 102 (control unit 54) displays the reference values ​​(lower limit and upper limit) corresponding to the internal standard markers (LM and UM) in the gel image display 94, regardless of the interval rejection of the values ​​on the scale 94a. Specifically, the analysis device 102 displays "LM" at the position corresponding to the size of LM and "UM" at the position corresponding to the size of UM on the scale 94a. In addition, the display of "UM" and "LM" is also in a manner that does not overlap with the values ​​on the scale 94a.

[0069] Furthermore, in the gel image display 94, when multiple resolution results are displayed in an arrangement by performing multiple measurements, if the width of the display area in the arrangement direction (horizontal) is insufficient, the displayed resolution results are changed by scrolling the display horizontally.

[0070] <Details of the zoomed-in display status>

[0071] In addition, such as Figure 6As shown, in the magnified display state, the analysis device 102 (control unit 54) is configured such that the sample well position display 91, the measurement waveform display 92, and the peak table 93 are not displayed, and the result display area 52a of the display unit 52 only magnifies and displays the gel image display 94 in the multiple analysis result confirmation display.

[0072] In this embodiment, the analysis device 102 (control unit 54) ensures that the size of each analysis result displayed in the arrangement direction (horizontal direction) in the gel image display 94 is the same size in both the detailed display state and the magnified display state. Therefore, the analysis device 102 is configured to display more analysis results in the magnified display state compared to the detailed display state.

[0073] That is, in the magnified display state, the resolution device 102 is configured to magnify only the vertical dimension of the resolution results in the gel image display 94, without magnifying the horizontal dimension. Therefore, compared to the detailed display state, the magnified display state displays more resolution results of the gel image display 94 in the result display area 52a of the display unit 52. Furthermore, in the magnified display state, if the width of the display area is insufficient, the displayed resolution results are changed by scrolling the display horizontally.

[0074] Furthermore, in this embodiment, the analysis device 102 (control unit 54) is configured such that the interval rejection of the values ​​of scale 94a in the gel image display 94 is different in the detailed display state and the magnified display state. Specifically, the analysis device 102 is configured such that the number of values ​​displayed in the magnified display state is greater than the number of values ​​displayed in the detailed display state, thus making the interval rejection of the number of values ​​displayed in scale 94a different in the detailed display state and the magnified display state. Moreover, the display size of the values ​​of scale 94a is the same in both the detailed display state and the magnified display state.

[0075] Furthermore, in the magnified display state, the analysis device 102 (control unit 54) is configured to eliminate the number of displayed values ​​at intervals based on the state where unevenness in the display intervals of the values ​​displayed in the scale 94a is suppressed. That is, in this embodiment, the analysis device 102 is configured to change the interval elimination degree of the values ​​corresponding to the scale 94a when switching between the detailed display state and the magnified display state, based on the state where unevenness in the display of the values ​​displayed in the scale 94a is suppressed.

[0076] Specifically, when switching from detailed display mode to magnified display mode, the analysis device 102 magnifies the display (image) of multiple analysis results in the gel image display 94 vertically. At this time, the analysis device 102 changes the interval rejection rate by increasing the number of displays for the values ​​displayed in the scale 94a, so that the values ​​do not overlap and the unevenness of the display interval is suppressed.

[0077] Furthermore, in this embodiment, even in the magnified display state, the analysis device 102 (control unit 54) displays the reference values ​​(lower limit and upper limit) corresponding to the internal standard marker substances (LM and UM) regardless of the interval rejection of the number of values ​​displayed in the scale 94a. That is, in both the detailed display state and the magnified display state, the display of "LM" indicating the lower limit of the size and the display of "UM" indicating the upper limit of the size are displayed as the references (lower limit and upper limit) in the scale 94a of the gel image display 94.

[0078] (Regarding electrophoretic analysis methods)

[0079] Next, refer to Figure 7 The electrophoretic analysis method using the electrophoresis system 100 in this embodiment will be explained. Furthermore, the control processing in steps 201 to 209 is performed by the control unit 54 (analysis device 102) executing the electrophoretic analysis program 53a stored in the storage unit 53.

[0080] First, in step 201, measurement condition information for performing the measurement is acquired. Specifically, information such as the sample well information indicating the sample wells 70a and 71a on which the test objects to be measured are arranged, the arrangement table information indicating the measurement order, and the information indicating the type of test objects (reference sample and test object) arranged in the sample wells 70a and 71a, along with measurement condition information including information indicating the magnitude and duration of the voltage applied by the voltage application unit 20, is acquired (set). Furthermore, this information can be acquired either based on input operations to the operation unit 51 or from information pre-stored in the storage unit 53, etc.

[0081] Next, in step 202, a drive signal containing the acquired measurement condition information is sent to the control unit 40 of the electrophoresis apparatus 101. In addition to the measurement condition information, the drive signal also includes the acquired sample well information and arrangement table information. Based on this drive signal, in the electrophoresis apparatus 101, each measurement object in the specified sample wells 70a and 71a is subjected to electrophoresis-based measurements using chips 60a to 60c in a predetermined sequence.

[0082] Next, in step 203, a measurement value 111 is obtained based on the measurement performed by the measurement unit 30 of the electrophoresis apparatus 101.

[0083] Next, in step 204, analysis is performed based on the obtained measurement value 111. Specifically, based on the obtained measurement value 111, the components of the analyte separated by electrophoresis are analyzed.

[0084] Next, in step 205, based on the analysis of the components of the analyte, the analysis results confirmation display in the detailed display state is displayed in the result display area 52a of the display unit 52. Specifically, multiple analysis results confirmation displays, including the sample well position display 91, the measurement waveform display 92, the peak table 93, and the gel image display 94, are displayed in the result display area 52a of the display unit 52.

[0085] Next, in step 206, it is determined whether an input operation for switching between detailed display mode and zoomed display mode has been received. Specifically, based on the operation signal from the operation unit 51 and the selection operation of the switch button display 52b shown on the display unit 52, it is determined whether an input operation for switching between detailed display mode and zoomed display mode has been received. If it is determined that an input operation for switching between detailed display mode and zoomed display mode has been received, the process proceeds to step 207. If it is not determined that an input operation for switching between detailed display mode and zoomed display mode has been received, the control processing remains pending while the detailed display mode continues to be displayed.

[0086] In step 207, based on the input operation received by the operation unit 51, the display is switched from detailed display mode to magnified display mode. Specifically, the sample well position display 91, the measurement waveform display 92, and the peak table 93 are not displayed, and only the gel image display 94 is magnified in the result display area 52a of the display unit 52.

[0087] Next, in step 208, it is determined whether an input operation to switch between detailed display mode and zoomed display mode has been received. Specifically, similar to step 206, based on the operation signal from the operation unit 51 and the selection operation of the switch button display 52b shown on the display unit 52, it is determined whether an input operation to switch between detailed display mode and zoomed display mode has been received. If it is determined that an input operation to switch between detailed display mode and zoomed display mode has been received, the process proceeds to step 209. If it is not determined that an input operation to switch between detailed display mode and zoomed display mode has been received, the control processing remains pending while the zoomed display mode continues to be displayed.

[0088] In step 209, based on the input operation received by the operation unit 51, the display is switched back from the magnified display state to the detailed display state. The display of the result display area 52a of the display unit 52 in the detailed display state is the same as in step 205.

[0089] Alternatively, the switch between detailed display mode and magnified display mode can be performed again after step 209. Alternatively, after analyzing the components of the measured object in step 204, the magnified display mode can be used to confirm the analysis results instead of the detailed display mode.

[0090] (Effects of this implementation method)

[0091] In this embodiment, the following effects can be obtained.

[0092] In the electrophoresis system 100 and electrophoresis apparatus 101 of this embodiment, as described above, switching between a detailed display state and a magnified display state is achieved. The detailed display state displays multiple resolution result confirmation displays, including at least the gel image display 94, in the result display area 52a of the display unit 52. The magnified display state magnifies only the gel image display 94 among the multiple resolution result confirmation displays in the result display area 52a of the display unit 52. Therefore, by switching between the detailed display state and the magnified display state, it is possible to switch between a state where multiple resolution result confirmation displays are displayed in the result display area 52a of the display unit 52 and a state where only the gel image display 94 is displayed in the result display area 52a of the display unit 52. Thus, when multiple bands with similar values ​​(sizes) exist in the band pattern of the gel image display 94 in the detailed display state, switching to the magnified display state can be used to magnify the gel image display 94. As a result, the visibility of the distribution of components separated by electrophoresis in the gel image display 94 can be improved.

[0093] Furthermore, in the above embodiments, further effects can be obtained by configuring it as follows.

[0094] That is, in this embodiment, as described above, the analysis device 102 is configured to reduce and periodically eliminate the number of values ​​corresponding to scale 94a displayed in the gel image display 94 so that the values ​​do not overlap, and the analysis device 102 is configured to ensure that the degree of interval elimination of the number of displayed values ​​is different in the detailed display state and the magnified display state. According to this structure, in both the detailed display state and the magnified display state, by reducing and periodically eliminating the values ​​of scale 94a in the gel image display 94 so that the values ​​of scale 94a in the gel image display 94 do not overlap, the decrease in the visibility of the values ​​of scale 94a in the gel image display 94 can be suppressed. Therefore, in both the detailed display state and the magnified display state of the gel image display 94, the visibility of the distribution of the separated components can be improved, and the specific values ​​representing the separated components (sizes) can be easily confirmed.

[0095] Furthermore, in this embodiment, as described above, the analysis device 102 is configured such that, in both the detailed display state and the magnified display state, numerical values ​​are displayed at a common display size, and the interval between the displayed numerical values ​​is different, with the number of displayed numerical values ​​in the magnified display state being greater than that in the detailed display state. According to this configuration, since more numerical values ​​are displayed at scale 94a in the gel image display 94 in the magnified display state, the visibility of the distribution of the separated components in the gel image display 94 is improved, and the specific numerical values ​​representing the separated components (sizes) can be confirmed in more detail. Additionally, since the numerical values ​​at scale 94a are displayed at a common display size in both the detailed display state and the magnified display state, the numerical values ​​at scale 94a displayed in the detailed display state do not decrease in size. Therefore, the decrease in the visibility of the numerical values ​​in the detailed display state can be suppressed.

[0096] Furthermore, in this embodiment, as described above, the electrophoresis apparatus 101 is configured to separate each of the multiple test objects by electrophoresis, and the analysis apparatus 102 is configured to display a gel image display 94 in the result display area 52a of the display unit 52, which displays multiple analysis results showing the distribution of components of each of the multiple test objects. The analysis apparatus 102 is also configured to ensure that the size of each analysis result in the arrangement direction is the same in both the detailed display state and the magnified display state, thereby displaying more analysis results in the magnified display state compared to the detailed display state. According to this structure, by switching from the detailed display state to the magnified display state, the visibility of the gel image display 94 can be improved, and since more analysis results are displayed in the magnified display state compared to the detailed display state, more analysis results can be visually identified at a glance. Therefore, when comparing multiple analysis results with each other, switching from the detailed display state to the magnified display state makes it easier to compare more analysis results with each other.

[0097] Furthermore, in this embodiment, as described above, the electrophoresis apparatus 101 is configured to separate the analyte containing an internal standard marker substance that serves as a reference in the analysis of the components of the analyte by electrophoresis, and the analysis apparatus 102 is configured to display, in both detailed display and magnified display states, reference values ​​(LM and UM) corresponding to the internal standard marker substance in the gel image display 94. With this configuration, even when switching between detailed display and magnified display states, the display of reference values ​​(LM and UM) corresponding to the internal standard marker substance can be maintained in the gel image display 94. Therefore, even when the interval rejection of the values ​​at the scale 94a in the gel image display 94 changes due to switching between detailed display and magnified display states, the display of reference values ​​corresponding to the internal standard marker substance can be confirmed. Thus, the reference of the separated components can be easily confirmed regardless of the size of the gel image display 94.

[0098] Furthermore, in this embodiment, as described above, the analysis device 102 is configured such that the unevenness of the display intervals of the values ​​in the gel image display 94 is suppressed, and the degree of rejection of the number of displayed values ​​is different in the detailed display state and the magnified display state. According to this configuration, the unevenness of the values ​​of scale 94a in the gel image display 94 is suppressed in both the detailed display state and the magnified display state, thus enabling the display of values ​​in the gel image display 94 with approximately equal display intervals of the values ​​of scale 94a. Therefore, compared to the case where the values ​​of scale 94a are displayed unevenly in the gel image display 94, it is easier to grasp the distribution of the specific values ​​of the separated components (sizes).

[0099] Furthermore, in this embodiment, as described above, the electrophoresis apparatus 101 is configured to separate a reference sample (as the object of measurement with known components) and a test sample (as the object of measurement with unknown components) by electrophoresis. The analysis apparatus 102 is configured to acquire numerical values ​​based on the analysis of the components of the reference sample, and the analysis apparatus 102 is configured to have different intervals for displaying the number of values ​​acquired based on the analysis of the components of the reference sample in detailed display mode and magnified display mode. Based on this structure, by checking the values ​​of the scale 94a on the gel image display 94, it is easy to determine which component in the reference sample with known components the separated components of the test sample with unknown components correspond to. Therefore, by checking the values ​​of the scale 94a displayed in the gel image display 94, it is easy to determine the specific values ​​used to represent the separated components (sizes) of the test sample.

[0100] Furthermore, in this embodiment, as described above, the electrophoresis apparatus 101 is configured to separate each of the multiple test objects by electrophoresis, and the analysis apparatus 102 is configured to, in the detailed display state, display multiple analysis result confirmation displays in the result display area 52a of the display unit 52, including a measurement waveform display 92 showing the time series values ​​of the measurement value 111, a sample well position display 91 showing the position of each of the multiple sample wells 70a and 71a arranged for each of the multiple test objects, and a gel image display 94 arranging multiple analysis results showing the distribution of the components of each of the multiple test objects. In the magnified display state, the analysis apparatus 102 is configured not to display the measurement waveform display 92 and the sample well position display 91, but to magnify and display only the gel image display 94 in the multiple analysis result confirmation displays in the result display area 52a of the display unit 52. Based on this structure, in the detailed display state, the gel image display 94 containing the analysis results of the components of the analyte, the positions of the sample wells 70a and 71a where the analyte is located, and the waveform of the measured value 111 can all be confirmed simultaneously. Therefore, the detailed analysis of the separated analyte can be confirmed. Thus, by switching between the detailed display state and the magnified display state, it is possible to easily switch between confirming the detailed analysis and confirming the improved visibility of the gel image display 94, depending on the situation.

[0101] Furthermore, in this embodiment, as described above, the electrophoresis system 100 includes an operation unit 51 for receiving input operations, and the analysis device 102 is configured such that, in a detailed display state, the result display area 52a of the display unit 52 displays a gel image display 94 showing multiple analysis results arranged in a row, and a measurement waveform display 92 corresponding to one analysis result selected from the multiple analysis results in the gel image display 94 based on the operation received by the operation unit 51. The analysis device 102 is also configured to switch between a detailed display state and a magnified display state based on the operation received by the operation unit 51. With this configuration, the operator analyzing the test object separated by electrophoresis can easily switch between the detailed display state and the magnified display state by operating the operation unit 51. Additionally, by selecting one analysis result from the gel image display 94 in the detailed display state, the operator can easily confirm the measurement waveform display 92 corresponding to the selected analysis result. As a result, the following actions can be easily switched by inputting an operation to the operation unit 51: confirming the magnified gel image display 94 in magnified display mode; and confirming the measurement waveform display 92 corresponding to a resolution result selected from the gel image display 94 in detailed display mode.

[0102] (Effects of the electrophoretic analysis method and procedure in this embodiment)

[0103] In the electrophoretic analysis method and electrophoretic analysis procedure 53a of this embodiment, the following effects can be obtained.

[0104] In the electrophoretic analysis method and procedure 53a of this embodiment, by configuring it as described above, it switches between a detailed display state and a magnified display state. The detailed display state displays multiple analysis result confirmation displays, including at least the gel image display 94, in the result display area 52a of the display unit 52. The magnified display state magnifies only the gel image display 94 among the multiple analysis result confirmation displays in the result display area 52a of the display unit 52. Therefore, by switching between the detailed display state and the magnified display state, it is possible to switch between a state where multiple analysis result confirmation displays are displayed in the result display area 52a of the display unit 52 and a state where only the gel image display 94 is displayed in the result display area 52a of the display unit 52. Thus, when multiple bands with similar values ​​(sizes) exist in the band pattern of the gel image display 94 in the detailed display state, it is possible to switch to the magnified display state to magnify the gel image display 94. As a result, an electrophoretic analysis method and procedure 53a that improves the visibility of the distribution of components separated by electrophoresis in the gel image display 94 can be provided.

[0105] [Variation Example]

[0106] Furthermore, it should be understood that the embodiments disclosed herein are illustrative in all respects and not restrictive. The scope of the invention is shown by the claims rather than by the description of the foregoing embodiments, and includes all modifications (variations) within the meaning and scope equivalent to the claims.

[0107] For example, in the above embodiment, an example is shown where an analysis device 102 for analyzing the components of the target object is provided independently of the electrophoresis apparatus 101, but the present invention is not limited thereto. In the present invention, the electrophoresis apparatus 101 and the analysis device 102 may also be formed as one unit. Similarly, it is also possible that only the display unit 52 is formed as one unit with the electrophoresis apparatus 101. Alternatively, the display unit 52 may be configured separately from both the electrophoresis apparatus 101 and the analysis device 102.

[0108] Furthermore, in the above embodiment, an example was shown where the number of values ​​corresponding to scale 94a in the gel image display 94 was reduced and intermittently removed to prevent the values ​​from overlapping; however, the present invention is not limited to this. For example, the values ​​may be displayed in an overlapping state on scale 94a within a range that allows the values ​​in scale 94a to be identified. Alternatively, the display size of the values ​​may be changed in such a way that the values ​​in scale 94a do not overlap, without changing the number of values ​​displayed.

[0109] Furthermore, in the above embodiment, an example was shown in which the size of each of the multiple resolution results displayed in the arrangement direction (horizontal direction) in the gel image display 94 is the same size in both the detailed display state and the magnified display state, but the present invention is not limited thereto. For example, it is also possible that the size of the multiple resolution results displayed in the arrangement direction is different in the detailed display state and the magnified display state.

[0110] Furthermore, in the above embodiment, an example was shown in which, regardless of the number of values ​​displayed on scale 94a, the reference values ​​(lower limit and upper limit) corresponding to the internal standard markers (LM and UM) are displayed on scale 94a in both the detailed display state and the magnified display state; that is, "LM" and "UM" are displayed on scale 94a. However, the present invention is not limited to this. For example, only one of "LM" and "UM" may be displayed on the gel image display 94. Alternatively, instead of displaying textual information showing the reference values ​​corresponding to the internal standard markers, the specific numerical values ​​of the reference values ​​may be displayed.

[0111] Furthermore, in the above embodiment, an example was shown where the number of displayed values ​​was selectively eliminated to suppress unevenness in the display interval of the values ​​of scale 94a in the gel image display 94, but the present invention is not limited to this. For example, unevenness in the display interval of the values ​​of scale 94a may also exist. In addition, when switching from a magnified display state to a detailed display state, the displayed values ​​of scale 94a can be eliminated directly, or the state change interval elimination degree can be changed to suppress unevenness by displaying values ​​different from those displayed in the detailed display state compared to the magnified display state.

[0112] Furthermore, in the above embodiment, an example was shown where the value of scale 94a was obtained based on the analysis of a reference sample, but the present invention is not limited thereto. For example, scale 94a may also display a preset value. Alternatively, scale 94a may be set to a fixed interval, and the value displayed by scale 94a may be set to a fixed display interval. Additionally, the value of scale 94a may be the time (time) at which the peak of the measured waveform is detected for each component, rather than a value representing size.

[0113] Furthermore, in the above embodiment, an example was shown in which, in the detailed display state, the result display area 52a of the display unit 52 displays a display for confirming analytical results, including a sample well position display 91, a measurement waveform display 92, a peak table 93, and a gel image display 94. However, the present invention is not limited to this. For example, the display for confirming analytical results in the detailed display state may not include one or any two of the sample well position display 91, the measurement waveform display 92, and the peak table 93. In addition, the multiple displays for confirming analytical results displayed in the detailed display state may also include displays showing the measurement conditions or measurement order, etc.

[0114] Furthermore, in the above embodiment, an example is shown where the control unit 54 of the analysis apparatus 102 acquires the measurement value 111 output from the control unit 40 of the electrophoresis apparatus 101 for analysis, but the present invention is not limited thereto. For example, the analysis apparatus 102 may also be configured to analyze the components of the measurement object separated by electrophoresis by acquiring the measurement value 111 pre-stored in a storage device or storage medium such as the storage unit 53. That is, the analysis apparatus 102 may also analyze the measurement value 111 at a time different from the time when the measurement value 111 of the electrophoresis apparatus 101 is acquired.

[0115] Furthermore, in the above embodiments, an example is shown where chips 60a-60c are provided with a preparation flow path 63 for guiding the measurement object to the separation flow path 62, but the present invention is not limited thereto. For example, chips 60a-60c may be configured to have only the separation flow path 62 and not include the preparation flow path 63. Alternatively, the preparation flow path 63 may be configured to intersect the separation flow path 62 in a T-shape, rather than intersecting the separation flow path 62 in a through-flow shape (cross-shaped).

[0116] Furthermore, in the above embodiment, an example was shown where the electrophoresis apparatus 101 was configured to measure each of the multiple (3) chips 60a to 60c, but the present invention is not limited thereto. For example, one or two chips can be used to measure the object, or four or more chips can be used. In addition, even when the electrophoresis apparatus 101 is configured to measure each of the three chips 60a to 60c, it can also be configured to select only one or two chips for measurement.

[0117] Furthermore, in the above embodiment, an example of configuring the electrophoresis apparatus 101 to perform microchip electrophoresis has been shown, but the present invention is not limited thereto. For example, it may also be configured to perform capillary electrophoresis without using a microchip.

[0118] Furthermore, in the above embodiment, an example of obtaining the measurement value 111 of the analyte through fluorescence detection was shown, but the present invention is not limited thereto. For example, the separated components of the analyte can also be detected by using a reagent for staining.

[0119] [Way]

[0120] Those skilled in the art will understand that the exemplary embodiments described above are specific examples of the following approaches.

[0121] (Project 1)

[0122] An electrophoresis system comprising:

[0123] An electrophoresis apparatus, comprising a measuring unit that measures the test objects that have been separated by electrophoresis in a flow path including a separation flow path for separating the test objects;

[0124] An analytical apparatus that analyzes the components of a analyte, which have been separated by electrophoresis, based on the measured values ​​of the analyte determined by the measuring unit; and

[0125] The display unit, in the result display area, displays at least a plurality of analytical result confirmation displays, including gel images showing the distribution of components of the measured object as resolved by the analytical device.

[0126] The analysis device is configured to switch between a detailed display state and a magnified display state. The detailed display state is a state in which the result display area of ​​the display unit displays at least the plurality of analysis result confirmation displays including the gel image display. The magnified display state is a state in which the result display area of ​​the display unit magnifies only the gel image display among the plurality of analysis result confirmation displays.

[0127] (Project 2)

[0128] According to the electrophoresis system described in Project 1, among which,

[0129] The analysis device is configured to reduce and periodically eliminate the number of values ​​corresponding to the scale displayed in the gel image display so that the values ​​do not overlap, and the analysis device is configured to make the interval elimination degree of the number of displayed values ​​different in the detailed display state and the magnified display state.

[0130] (Project 3)

[0131] According to the electrophoresis system described in Project 2, among which,

[0132] The analysis device is configured such that, in both the detailed display state and the magnified display state, the numerical value is displayed at a common display size, and the interval between the display numbers of the numerical values ​​is different in such a way that the number of numerical values ​​displayed in the magnified display state is greater than the number of numerical values ​​displayed in the detailed display state.

[0133] (Project 4)

[0134] According to the electrophoresis system described in Project 2 or 3, among which,

[0135] The electrophoresis apparatus is configured to separate each of the multiple test objects by electrophoresis.

[0136] The analytical apparatus is configured such that the result display area of ​​the display unit displays a gel image in which multiple analytical results showing the distribution of components of each of the multiple measured objects are arranged, and the analytical apparatus is configured such that the size of each of the multiple analytical results displayed in the arrangement direction is the same size in both the detailed display state and the magnified display state, thereby displaying more analytical results in the magnified display state compared to the detailed display state.

[0137] (Project 5)

[0138] According to the electrophoresis system described in any of items 2 to 4, among which,

[0139] The electrophoresis apparatus is configured to separate the analyte, which contains an internal standard marker substance that serves as a reference in the analysis of the components of the analyte, by electrophoresis.

[0140] The analytical device is configured such that, regardless of the interval rejection of the number of displayed values, in both the detailed display state and the magnified display state, a reference value corresponding to the internal standard marker substance is displayed in the gel image display.

[0141] (Project 6)

[0142] According to the electrophoresis system described in any of items 2 to 5, among which,

[0143] The analysis device is configured such that the unevenness of the display interval of the values ​​in the gel image is suppressed, and the interval elimination degree of the number of displayed values ​​is different in the detailed display state and the magnified display state.

[0144] (Project 7)

[0145] According to the electrophoresis system described in any of items 2 to 6, among which,

[0146] The electrophoresis apparatus is configured to separate a reference sample (as the object of measurement with known composition) and an object sample (as the object of measurement with unknown composition) by electrophoresis.

[0147] The analytical device is configured to obtain the numerical value based on the analysis of the composition of the reference sample, and the analytical device is configured to make the interval rejection of the number of displayed values ​​obtained based on the analysis of the composition of the reference sample different in the detailed display state and the magnified display state.

[0148] (Project 8)

[0149] According to the electrophoresis system described in any one of items 1 to 7, among which,

[0150] The electrophoresis apparatus is configured to separate each of the multiple test objects by electrophoresis.

[0151] The analysis device is configured such that, in the detailed display state, the result display area of ​​the display unit displays a plurality of analysis result confirmation displays, including a measurement waveform display showing the time series of the measured values, a sample well position display showing the position of each sample well in a plurality of sample wells in which each of the plurality of measured objects is arranged, and a gel image display showing the distribution of components of each of the plurality of measured objects arranged in a plurality of analysis results. Furthermore, in the magnified display state, the measurement waveform display and the sample well position display are not displayed, but the result display area of ​​the display unit only magnifies and displays the gel image display in the plurality of analysis result confirmation displays.

[0152] (Project 9)

[0153] According to the electrophoresis system described in Project 8, among which,

[0154] It also has an operating unit for accepting input operations.

[0155] The analysis device is configured such that, in the detailed display state, the result display area of ​​the display unit displays a gel image display in which the plurality of analysis results are arranged and displayed, and a measurement waveform display corresponding to one of the analysis results selected from the plurality of analysis results in the gel image display based on an operation received by the operation unit, and the analysis device is configured to switch between the detailed display state and the magnified display state based on an operation received by the operation unit.

[0156] (Project 10)

[0157] An electrophoresis apparatus includes a measuring unit that measures the test objects, which have been separated by electrophoresis in a flow path including a separation flow path for separating the test objects.

[0158] The electrophoresis apparatus is configured to switch between a detailed display state and a magnified display state. The detailed display state is a state in which the result display area of ​​the display unit displays at least a plurality of analytical result confirmation displays, including a gel image display, wherein the gel image display shows the distribution of the components of the analyte determined based on the measurement values ​​of the analyte measured by the measurement unit. The magnified display state is a state in which the result display area of ​​the display unit displays only the gel image display among the plurality of analytical result confirmation displays.

[0159] (Project 11)

[0160] An electrophoretic analysis method includes the following steps:

[0161] Based on the measured values ​​obtained by measuring the analyte separated by electrophoresis in a flow path including a separation flow path for separating the analyte, the components of the analyte separated by electrophoresis are analyzed; and

[0162] The display can switch between a detailed display state and a magnified display state. The detailed display state is a state in which the result display area of ​​the display unit displays at least a plurality of analytical result confirmation displays, including a gel image display, wherein the gel image display shows the distribution of the components of the measured object that have been analyzed. The magnified display state is a state in which the result display area of ​​the display unit displays only the gel image display among the plurality of analytical result confirmation displays.

[0163] (Project 12)

[0164] An electrophoretic analysis program that causes a computer to perform the following steps:

[0165] Based on the measured values ​​obtained by measuring the analyte separated by electrophoresis in a flow path including a separation flow path for separating the analyte, the components of the analyte separated by electrophoresis are analyzed; and

[0166] The display can switch between a detailed display state and a magnified display state. The detailed display state is a state in which the result display area of ​​the display unit displays at least a plurality of analytical result confirmation displays, including a gel image display, wherein the gel image display shows the distribution of the components of the measured object that have been analyzed. The magnified display state is a state in which the result display area of ​​the display unit displays only the gel image display among the plurality of analytical result confirmation displays.

Claims

1. An electrophoresis system, comprising: An electrophoresis apparatus, comprising a measuring unit that measures the test objects that have been separated by electrophoresis in a flow path including a separation flow path for separating the test objects; An analytical apparatus that analyzes the components of the analyte, which have been separated by electrophoresis, based on the measured values ​​of the analyte determined by the measuring unit. as well as The display unit, in the result display area, displays at least a plurality of analytical result confirmation displays, including gel images showing the distribution of components of the measured object as resolved by the analytical device. The analysis device is configured to switch between a detailed display state and a magnified display state. The detailed display state displays at least the plurality of analysis result confirmation displays, including the gel image display, in the result display area of ​​the display unit. The magnified display state displays only the gel image display among the plurality of analysis result confirmation displays in the result display area of ​​the display unit. The electrophoresis apparatus is configured to separate each of the multiple test objects by electrophoresis. The analysis device is configured such that the result display area of ​​the display unit displays a gel image that shows the distribution of components of each of the plurality of measured objects in an arranged manner, and the analysis device is configured to display more analysis results in the magnified display state compared to the detailed display state.

2. The electrophoresis system according to claim 1, wherein, The analysis device is configured to reduce and periodically eliminate the number of values ​​corresponding to the scale displayed in the gel image display so that the values ​​do not overlap, and the analysis device is configured to make the interval elimination degree of the number of displayed values ​​different in the detailed display state and the magnified display state.

3. The electrophoresis system according to claim 2, wherein, The analysis device is configured such that, in both the detailed display state and the magnified display state, the numerical value is displayed at a common display size, and the interval between the display numbers of the numerical values ​​is different in such a way that the number of numerical values ​​displayed in the magnified display state is greater than the number of numerical values ​​displayed in the detailed display state.

4. The electrophoresis system according to claim 1, wherein, The analysis device is configured such that the size of each analysis result in the arrangement direction is the same in both the detailed display state and the magnified display state, thereby displaying more analysis results in the magnified display state compared to the detailed display state.

5. The electrophoresis system according to claim 2, wherein, The electrophoresis apparatus is configured to separate the analyte, which contains an internal standard marker substance that serves as a reference in the analysis of the components of the analyte, by electrophoresis. The analytical device is configured such that, regardless of the interval rejection of the number of displayed values, in both the detailed display state and the magnified display state, a reference value corresponding to the internal standard marker substance is displayed in the gel image display.

6. The electrophoresis system according to claim 2, wherein, The analysis device is configured such that the unevenness of the display interval of the values ​​in the gel image is suppressed, and the interval elimination degree of the number of displayed values ​​is different in the detailed display state and the magnified display state.

7. The electrophoresis system according to claim 1, wherein, The analysis device is configured such that, in the detailed display state, the result display area of ​​the display unit displays a plurality of analysis result confirmation displays, including a measurement waveform display showing the time series of the measured values, a sample well position display showing the position of each sample well in a plurality of sample wells in which each of the plurality of measured objects is arranged, and a gel image display showing the distribution of components of each of the plurality of measured objects arranged in a plurality of analysis results. Furthermore, in the magnified display state, the measurement waveform display and the sample well position display are not displayed, but the result display area of ​​the display unit only magnifies and displays the gel image display in the plurality of analysis result confirmation displays.

8. The electrophoresis system according to claim 7, wherein, It also has an operating unit for accepting input operations. The analysis device is configured such that, in the detailed display state, the result display area of ​​the display unit displays a gel image display in which the plurality of analysis results are arranged and displayed, and a measurement waveform display corresponding to one of the analysis results selected from the plurality of analysis results in the gel image display based on an operation received by the operation unit, and the analysis device is configured to switch between the detailed display state and the magnified display state based on an operation received by the operation unit.

9. An electrophoresis system, comprising: An electrophoresis apparatus, comprising a measuring unit that measures the test objects that have been separated by electrophoresis in a flow path including a separation flow path for separating the test objects; An analytical apparatus that analyzes the components of the analyte, which have been separated by electrophoresis, based on the measured values ​​of the analyte determined by the measuring unit. as well as The display unit, in the result display area, displays at least a plurality of analytical result confirmation displays, including gel images showing the distribution of components of the measured object as resolved by the analytical device. The analysis device is configured to switch between a detailed display state and a magnified display state. The detailed display state displays at least the plurality of analysis result confirmation displays, including the gel image display, in the result display area of ​​the display unit. The magnified display state displays only the gel image display among the plurality of analysis result confirmation displays in the result display area of ​​the display unit. The analysis device is configured to reduce and periodically eliminate the number of displayed values ​​corresponding to the scale in the gel image display so that the values ​​do not overlap, and the analysis device is configured to ensure that the interval of elimination of the number of displayed values ​​is different in the detailed display state and the magnified display state. The electrophoresis apparatus is configured to separate a reference sample (as the object of measurement with known composition) and an object sample (as the object of measurement with unknown composition) by electrophoresis. The analytical device is configured to obtain the numerical value based on the analysis of the composition of the reference sample, and the analytical device is configured to make the interval rejection of the number of displayed values ​​obtained based on the analysis of the composition of the reference sample different in the detailed display state and the magnified display state.

10. An electrophoresis apparatus comprising a measuring unit that measures the test object after it has been separated by electrophoresis in a flow path including a separation flow path for separating the test object. The electrophoresis apparatus is configured to switch between a detailed display state and a magnified display state. The detailed display state is a state where the result display area of ​​the display unit displays at least a plurality of analytical result confirmation displays, including a gel image display. The gel image displays the distribution of components of the test object as resolved based on the measured values ​​of the test object obtained using the measuring unit. The magnified display state is a state in which the result display area of ​​the display unit displays only the gel image displayed in the multiple resolution result confirmation display. The electrophoresis apparatus is configured to separate each of the multiple test objects by electrophoresis. The electrophoresis apparatus is configured such that the result display area of ​​the display unit displays a gel image showing multiple analytical results that arrange and display the distribution of components of each of the multiple measured objects, and is configured to display more analytical results in the magnified display state compared to the detailed display state.

11. An electrophoretic analysis method, comprising the following steps: Based on the measured values ​​obtained by measuring a plurality of the analytes separated by electrophoresis in a flow path including a separation flow path for separating the analytes, the components of the plurality of analytes separated by electrophoresis are analyzed; as well as The system switches between a detailed display state and a magnified display state. The detailed display state displays multiple confirmation displays of analytical results, including at least a gel image, in the result display area of ​​the display unit. The gel image displays show the distribution of components of the analyzed object. The magnified display state displays only the gel image from the multiple confirmation displays of analytical results in the result display area of ​​the display unit. The step of switching between the detailed display state and the magnified display state includes the following steps: displaying the gel image in the result display area of ​​the display unit, which displays multiple analytical results showing the distribution of components of each of the multiple measured objects in an arranged manner; and switching between the detailed display state and the magnified display state, such that more analytical results are displayed in the magnified display state compared to the detailed display state.

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