A Chinese medicine composition for treating avian influenza and its application

Abstract

The invention provides a Chinese medicine composition for treating avian influenza and its application, and relates to the technical field of Chinese veterinary medicine. The Chinese medicine composition selects dandelion, isatis indigo leaf, liquorice, forsythia, gardenia, rhubarb, mirabilite, atractylodes, scutellaria, rhubarb, anemarrhena, and calamus, and the medicines are used together to play the effects of clearing away heat, detoxicating, and cooling blood. The Chinese medicine composition of the invention has obvious antiviral effect on avian influenza virus, has a high cure rate for avian influenza, reduces the mortality rate of poultry, significantly improves the titer level of multiple influenza antibodies in the body and maintains a high level for a long time, effectively improves the body's immunity, and enhances the body's ability to resist viral infection.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine veterinary drugs, and in particular to a traditional Chinese medicine composition for treating avian influenza and its application. Background Art

[0002] Avian influenza (AI) is a disease caused by avian influenza virus (AIV) that mainly infects the respiratory system. It often causes poultry to become ill or even die, and can also infect mammals and humans. Avian influenza has a wide range of transmission, a fast transmission speed, and a variety of virus subtypes. Once poultry is infected with this virus, it is easy to be mixed with other pathogens (such as Escherichia coli, Newcastle disease virus, etc.). It is also possible that racial mutations will occur between multiple subtypes of AIV, which will bring serious harm to the poultry industry and pose a serious threat to human public health and safety. At present, in addition to strengthening daily comprehensive management and routine disinfection, vaccination is the most important means of prevention for the prevention and control of avian influenza. However, due to the large number of serotypes of AIV, the cross-protection function between different types is weak, and even different strains of the same serotype have large differences; in addition, there are also problems such as the window period for immune antibody production. Western medicines such as neuraminidase (NA) inhibitors (such as Tamiflu) are widely used in human influenza, but they also have the problem of easy drug resistance.

[0003] Traditional Chinese veterinary medicine has a history of thousands of years, and it has unique advantages such as multiple targets, low drug resistance, few side effects, many types, and low cost. It is expected to play an important role in the fight against AIV. Traditional Chinese medicine is a natural medicine, and it also contains a variety of effective biologically active substances such as alkaloids and polysaccharides. In addition, it also contains minerals, vitamins and unknown nutritional factors, which can play a variety of roles in the prevention, treatment and nutrition of diseases. At present, there are many studies at home and abroad reporting that Chinese herbal medicine has a certain inhibitory effect on AIV. There are also Chinese patent medicines for the prevention and treatment of avian influenza on the market, but there are problems such as narrow universal range, slow absorption, unclear inhibitory effect on viruses, and unsatisfactory treatment effects. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies in the prior art, the present invention provides a Chinese medicine composition for treating avian influenza and its application. The Chinese medicine composition has a definite therapeutic effect on avian influenza, has no toxic side effects on the body, has a significant inhibitory effect on viruses, and has a significant enhancing effect on the body's immune function.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] On the one hand, the present invention provides a traditional Chinese medicine composition for treating avian influenza, which is composed of the following traditional Chinese medicine raw materials in parts by weight: 10-30 parts of dandelion, 10-30 parts of isatis indigotica, 5-15 parts of licorice, 10-20 parts of forsythia, 5-15 parts of gardenia, 10-20 parts of rhubarb, 5-15 parts of sodium sulfate, 10-30 parts of atractylodes, 15-30 parts of scutellaria, 5-15 parts of anemarrhena, and 10-20 parts of calamus.

[0009] Furthermore, the Chinese medicine composition is composed of the following Chinese medicine raw materials in parts by weight: 20-30 parts of dandelion, 10-20 parts of isatis indigotica, 8-12 parts of licorice, 14-18 parts of forsythia, 5-10 parts of gardenia, 12-16 parts of rhubarb, 5-10 parts of sodium sulfate, 15-25 parts of atractylodes, 20-30 parts of scutellaria, 8-12 parts of anemarrhena, and 12-16 parts of calamus.

[0010] Furthermore, the Chinese medicine composition is composed of the following Chinese medicine raw materials in parts by weight: 26 parts of dandelion, 14 parts of isatis indigotica, 8 parts of licorice, 15 parts of forsythia, 8 parts of gardenia, 15 parts of rhubarb, 8 parts of sodium sulfate, 22 parts of atractylodes, 26 parts of scutellaria, 10 parts of anemarrhena, and 14 parts of calamus.

[0011] In another aspect, the present invention also provides an application of a traditional Chinese medicine composition for treating avian influenza, wherein the traditional Chinese medicine composition is used to prepare a drug for treating avian influenza, and the traditional Chinese medicine composition is used to prepare any drug dosage form acceptable to an animal, including but not limited to powders, granules, decoctions, and oral liquids.

[0012] Pharmacological effects of each Chinese medicinal ingredient in the present invention

[0013] Dandelion: bitter and sweet in taste, cold in nature, enters the liver and stomach meridians; has the effects of clearing away heat and detoxifying, reducing swelling and dispersing nodules, and promoting diuresis and relieving stranguria; used for carbuncle, breast abscess, scrofula, red eyes, sore throat, lung abscess, intestinal abscess, damp-heat jaundice, hot stranguria and other diseases; it contains effective ingredients such as flavonoids, coumarins, phenolic acids, terpenes, sterols and polysaccharides. Clinical studies have shown that it has pharmacological effects such as antibacterial, antioxidant, anti-inflammatory, anti-tumor and gastrointestinal protection.

[0014] Folium Isatidis: bitter in taste, cold in nature, enters the liver, heart, stomach and spleen meridians; has the effects of clearing away heat and detoxifying, cooling blood and removing spots; used for fever, high fever, thirst, rashes, jaundice, diarrhea, erysipelas, mouth sores and other symptoms; it contains chemical components such as indoles, quinazolinones, and mustard glycosides, and is clinically used to treat various viral influenza, mumps, viral hepatitis and other infectious diseases, with antibacterial, antiviral, anti-endotoxin activity, anti-cancer, immune enhancement and other pharmacological effects.

[0015] Licorice: sweet in taste, neutral in nature, enters the heart, spleen, lung, and stomach meridians; has the effects of tonifying the spleen and replenishing qi, clearing away heat and detoxifying, removing phlegm and relieving cough, relieving acute pain, and harmonizing various medicines; used for spleen and stomach weakness, fatigue, palpitations and shortness of breath, cough with sputum, carbuncles, sores and other symptoms. Licorice is also good at harmonizing the properties of medicines and alleviating the toxicity of drugs.

[0016] Forsythia: bitter in taste, slightly cold in nature, enters the heart, lung and small intestine meridians; has the effects of clearing away heat and detoxifying, reducing swelling and dispersing nodules; used for breast pain, erysipelas, wind-heat cold, the initial stage of febrile disease, high fever and thirst, coma and rash, etc.; Forsythia contains active ingredients such as phenylethanol glycosides, lignans and their glycosides, pentacyclic triterpenes, phenolic acids, volatile oils, etc.; has antibacterial, antioxidant, antiviral, anti-inflammatory, antipyretic and analgesic effects.

[0017] Gardenia: bitter, cold in nature, enters the heart meridian, lung meridian, and triple burner meridian; has the effects of purging fire and relieving restlessness, clearing heat and removing dampness, cooling blood and detoxifying; used to treat fever and restlessness, damp-heat jaundice, stranguria and pain, red and swollen eyes, fire poison sores and other symptoms; Gardenia contains chemical components such as cyclopentane ether terpenes, diterpenes, flavonoids and organic acid esters, and the gardenia glycosides it contains have multiple effects such as anti-infection, immunomodulation and antioxidant. Gardenia glycosides can restore the dynamic balance of pro-inflammatory / anti-inflammatory cytokines, regulate the function and activation of immune cells, reduce the production of inflammatory transmitters and inflammatory factors, and regulate multiple signaling pathways to play anti-infection and immunomodulatory effects.

[0018] Rhubarb: It has the function of purging and attacking accumulation, clearing away heat and purging fire, cooling blood and detoxifying, removing blood stasis and promoting menstruation, and removing dampness and relieving jaundice. It is used for constipation caused by excess heat accumulation, vomiting and bleeding due to blood heat, red eyes and swollen throat, carbuncle, abdominal pain due to intestinal carbuncle, amenorrhea caused by blood stasis, postpartum blood stasis, traumatic injury, dysentery caused by damp heat, jaundice and red urine, stranguria, and edema.

[0019] Glauber's Salt: It has a laxative effect, moisturizes dryness and softens hard masses, clears away heat and reduces swelling. It is used for stagnation of excess heat, abdominal distension and pain, dry and hard stools, and swollen and painful intestinal abscesses. It is also used externally to treat breast abscesses and swollen and painful hemorrhoids.

[0020] Atractylodes: bitter, sweet, and warm in nature, it enters the spleen and stomach meridians; it has the effects of strengthening the spleen and replenishing Qi, drying dampness and promoting diuresis, stopping sweating, and calming the fetus; it is often used for symptoms such as spleen deficiency, poor appetite, diarrhea, loose stools, and fetal movement disorder; its main chemical components include volatile oils, polysaccharides, and lipids, which have anti-tumor, anti-inflammatory, and digestive system regulation effects.

[0021] Scutellaria baicalensis: bitter in taste, cold in nature, enters the spleen and lung meridians; it contains baicalin, baicalein, wogonin, baicalein I, II, norwogonin, volatile oil, phenylethanol glycosides, etc., which can clear away heat and dampness, purge fire and detoxify, stop bleeding, stabilize pregnancy, lower blood pressure, kill bacteria, kill viruses, kill fungi, etc. Baicalin and baicalein contained in Scutellaria baicalensis have good antioxidant function. Human medicine uses Scutellaria baicalensis to treat ischemic stroke (cerebral infarction) and Parkinson's disease, and veterinary medicine uses it to treat foot-and-mouth disease, blister disease, clear lung heat, and eliminate respiratory tract infections.

[0022] Anemarrhena: bitter in taste, cold in nature, enters the lung, stomach and kidney meridians; has the effects of clearing away heat and purging fire, nourishing yin and moistening dryness; used for symptoms such as thirst due to febrile disease, dry cough due to lung heat, hot flashes due to bone steaming, internal heat and thirst, dry intestinal constipation, etc.; the active ingredients contained in Anemarrhena are mainly saponins, bipyridones, lignin, alkaloids, polysaccharides, trace elements and a large amount of mucus, etc., which have anti-inflammatory, antiviral, platelet aggregation inhibition, blood sugar lowering, blood lipid lowering and other pharmacological effects.

[0023] Acorus calamus: pungent, slightly warm, belongs to the liver meridian; has the effects of opening the orifices, removing phlegm, regulating qi, activating blood circulation, dispersing wind, and removing dampness. It can treat epilepsy, phlegm syncope, fever coma, forgetfulness, qi stagnation and deafness, chest distress, stomachache, abdominal pain, wind-cold-dampness arthralgia, carbuncle swelling and poison, and traumatic injuries.

[0024] (III) Beneficial effects

[0025] The present invention provides a Chinese medicine composition for treating bird flu and its application. The Chinese medicine composition is prepared by rationally combining dandelion, isatis indigo leaf, liquorice, forsythia, gardenia, rhubarb, mirabilite, atractylodes macrocephala, scutellaria baicalensis, rhubarb rhizome, anemarrhena, and calamus according to the principle of syndrome differentiation and treatment in traditional Chinese medicine, giving full play to the effects of each medicine, and the combination of the medicines has the effects of clearing away heat, detoxicating, and cooling blood. The Chinese medicine combination of the present invention has obvious antiviral effect on bird flu virus, has a high cure rate for bird flu, reduces mortality, promotes the recovery of egg-laying performance, and can significantly improve the titer level of antibodies to multiple influenza in the body and maintain a high level for a long time, has a positive effect on the body's immune organs, effectively improves the body's immunity, and improves the body's ability to resist viral infection. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The bursal index corresponding to the blank control group, the western medicine control group, the traditional Chinese medicine control group and the traditional Chinese medicine group of the present invention ( Figure 1 A) Spleen index ( Figure 1 B) Thymus index ( Figure 1 C). DETAILED DESCRIPTION

[0027] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Example 1

[0029] A traditional Chinese medicine composition for treating avian influenza is composed of the following raw materials in parts by weight: 10 parts of dandelion, 10 parts of isatis indigotica, 5 parts of liquorice, 10 parts of forsythia, 5 parts of gardenia, 10 parts of rhubarb, 5 parts of sodium sulfate, 10 parts of atractylodes, 15 parts of scutellaria, 5 parts of anemarrhena, and 10 parts of calamus.

[0030] The Chinese medicine composition is used for preparing medicine for treating avian influenza, and is used for preparing any medicine dosage form acceptable to animals, including but not limited to powder, granule, decoction, and oral liquid.

[0031] Example 2

[0032] The difference between this embodiment and embodiment 1 is that:

[0033] The weight proportions of the raw materials are as follows: 30 parts of dandelion, 30 parts of isatis indigotica, 15 parts of licorice, 20 parts of forsythia, 15 parts of gardenia, 20 parts of rhubarb, 15 parts of sodium sulfate, 30 parts of atractylodes, 30 parts of scutellaria, 15 parts of anemarrhena, and 20 parts of calamus.

[0034] Example 3

[0035] The difference between this embodiment and embodiment 1 is that:

[0036] The weight proportions of the raw materials are as follows: 20 parts of dandelion, 10 parts of isatis indigotica, 8 parts of licorice, 14 parts of forsythia, 5 parts of gardenia, 12 parts of rhubarb, 5 parts of sodium sulfate, 15 parts of atractylodes, 20 parts of scutellaria, 8 parts of anemarrhena, and 12 parts of calamus.

[0037] Example 4

[0038] The difference between this embodiment and embodiment 1 is that:

[0039] The weight proportions of the raw materials are as follows: 30 parts of dandelion, 20 parts of isatis indigotica, 12 parts of licorice, 18 parts of forsythia, 10 parts of gardenia, 16 parts of rhubarb, 10 parts of sodium sulfate, 25 parts of atractylodes, 30 parts of scutellaria, 12 parts of anemarrhena, and 16 parts of calamus.

[0040] Example 5

[0041] The difference between this embodiment and embodiment 1 is that:

[0042] The weight proportions of the raw materials are as follows: 16 parts of dandelion, 22 parts of isatis indigotica, 6 parts of licorice, 14 parts of forsythia, 6 parts of gardenia, 12 parts of rhubarb, 6 parts of sodium sulfate, 14 parts of atractylodes, 20 parts of scutellaria, 7 parts of anemarrhena, and 11 parts of calamus.

[0043] Example 6

[0044] The difference between this embodiment and embodiment 1 is that:

[0045] The weight proportions of the raw materials are as follows: 22 parts of dandelion, 20 parts of isatis indigotica, 10 parts of licorice, 12 parts of forsythia, 10 parts of gardenia, 10 parts of rhubarb, 7 parts of sodium sulfate, 18 parts of atractylodes, 22 parts of scutellaria, 8 parts of anemarrhena, and 16 parts of calamus.

[0046] Example 7

[0047] The difference between this embodiment and embodiment 1 is that:

[0048] The weight proportions of the raw materials are as follows: 24 parts of dandelion, 18 parts of isatis indigotica, 8 parts of licorice, 18 parts of forsythia, 6 parts of gardenia, 13 parts of rhubarb, 6 parts of sodium sulfate, 16 parts of atractylodes, 28 parts of scutellaria, 9 parts of anemarrhena, and 12 parts of calamus.

[0049] Example 8

[0050] The difference between this embodiment and embodiment 1 is that:

[0051] The weight proportions of the raw materials are as follows: 26 parts of dandelion, 14 parts of isatis indigotica, 8 parts of licorice, 15 parts of forsythia, 8 parts of gardenia, 15 parts of rhubarb, 8 parts of sodium sulfate, 22 parts of atractylodes, 26 parts of scutellaria, 10 parts of anemarrhena, and 14 parts of calamus.

[0052] Test Example 1

[0053] The drugs of the present invention are reasonably compatible, and the effects of each drug are fully exerted. The drugs work together to enhance the efficacy. Due to the large amount of experimental data in the present invention, the prescriptions are adjusted many times and experiments are carried out. Here, only the antiviral effects of multiple prescriptions on H9N2 subtype AIV are listed and compared with the prescription of the present invention.

[0054] Prescription 1: 14 parts of Isatis indigotica, 8 parts of Licorice, 15 parts of Forsythia suspensa, 8 parts of Gardenia jasminoides, 15 parts of Rhubarb, 26 parts of Scutellaria baicalensis, 10 parts of Anemarrhena asphodeloides, and 14 parts of Acorus calamus.

[0055] Prescription 2: 26 parts of dandelion, 8 parts of liquorice, 15 parts of rhubarb, 8 parts of sodium sulfate, 22 parts of atractylodes, 26 parts of scutellaria, 10 parts of anemarrhena, and 14 parts of calamus.

[0056] Prescription 3: 26 parts of dandelion, 14 parts of isatis indigotica, 15 parts of forsythia, 8 parts of gardenia, 15 parts of rhubarb, 8 parts of sodium sulfate, 22 parts of atractylodes, 26 parts of scutellaria.

[0057] Prescription 4: 26 parts of dandelion, 14 parts of isatis indigotica, 8 parts of liquorice, 15 parts of forsythia, 8 parts of gardenia, 8 parts of sodium sulfate, 22 parts of atractylodes, 10 parts of anemarrhena, and 14 parts of calamus.

[0058] The prescription of the present invention includes 26 parts of dandelion, 14 parts of isatis indigotica, 8 parts of liquorice, 15 parts of forsythia, 8 parts of gardenia, 15 parts of rhubarb, 8 parts of sodium sulfate, 22 parts of atractylodes, 26 parts of scutellaria, 10 parts of anemarrhena and 14 parts of calamus.

[0059] Classic Chinese medicine formula: Qingwen Baidu San

[0060] The above Chinese medicine raw materials are washed, dried, crushed, and prepared into Chinese medicine stock solution according to the conventional decoction method, with a concentration of 200 mg / mL (each milliliter is equivalent to 200 mg of the original medicinal material), and stored in a refrigerator at 0-4°C for future use.

[0061] Western medicine: Oseltamivir phosphate granules are prepared into a 1 mg / mL concentration solution with sterile saline.

[0062] Test method:

[0063] The EID of 1H9N2 subtype AIV in chicken embryos 50 ) determination

[0064] The virus solution was diluted to 10 with sterile saline. -5 Up to 10 -9 , then inoculate different concentrations of virus liquid into 10-day-old SPF chicken embryos through the allantoic cavity, inoculate 0.1 mL per embryo, and inoculate 5 embryos at each concentration, then incubate in an incubator at 37.5±0.5°C and 60±5% humidity, observe every 24 hours, incubate for 120 hours, discard dead chicken embryos within 24 hours, measure the hemagglutination titer of dead embryos after 24 hours, and those with red blood cell agglutination of more than 4 holes are judged as positive. Determine virus EID according to the Reed-Muench method 50 .

[0065] Finally, the H9N2 subtype AIV was tested for chicken embryo EID 50 For 10 -8.5 / 0.1mL.

[0066] 2 Test on the anti-H9N2 subtype AIV activity of different Chinese medicine compositions and Western medicines

[0067] Forty-five 10-day-old SPF chicken embryos were selected and divided into 9 groups, 5 in each group, namely blank control group, virus control group, western medicine control group, Chinese medicine control group, and experimental groups 1-5; the blank control group was inoculated with 0.2 mL / egg of normal saline; the virus control group was inoculated with 0.1 mL 100 EID 50 Virus liquid / piece inoculated 5 chicken embryos; the Western medicine control group, the Chinese medicine control group and the experimental groups 1-5 were first inoculated with 0.1mL100 EID 50The virus solution was inoculated into 5 chicken embryos of each group and incubated for 2 hours. The western medicine control group was inoculated with 0.2 mL / piece of 1 mg / mL oseltamivir phosphate solution into 5 chicken embryos; the Chinese medicine control group and test groups 1-5 corresponded to the Chinese medicine stock solutions prepared by the classic Chinese medicine formula, formula 1-4 and the formula of the present invention, respectively, and the Chinese medicine stock solutions prepared by each formula were taken and inoculated into 5 chicken embryos of test groups 1-5 at 0.2 mL / piece; the chicken embryos of each group were incubated in an incubator at a temperature of 37.5±0.5°C and a humidity of 60±5% for 120 hours, the allantoic fluid was collected aseptically, and then a blood agglutination test was performed, and the blood agglutination test was performed according to a conventional method.

[0068] From the results in Table 1, it can be seen that the hemagglutination titers of the western medicine control group, the Chinese medicine control group and the test 1-5 groups are significantly lower than those of the virus control group (P<0.05), indicating that the drugs used in each group have a certain antiviral effect, and the lower the hemagglutination titer, the better the antiviral effect; compared with the Chinese medicine control group, the hemagglutination titers of the western medicine control group and the test 1-5 groups are significantly lower than those of the Chinese medicine control group (P<0.05); compared with the western medicine control group, the hemagglutination titer of the test 1-4 groups is significantly higher than that of the western medicine control group (P<0.05), and the hemagglutination titer of the test 5 group is equivalent to that of the western medicine control group, with no significant difference (P>0.05); the above results indicate that the antiviral effect of the Chinese medicine composition of the present invention on H9N2 subtype AIV is equivalent to that of oseltamivir phosphate, and is superior to the Chinese medicine composition circulated on the market, and when some of the Chinese medicines in the Chinese medicine composition of the present invention are missing, the ideal antiviral effect cannot be achieved. The Chinese medicine composition of the present invention is obtained by applying the principle of Chinese medicine dialectical treatment and reasonable compatibility, giving full play to the synergistic effect between the medicinal materials, and the ingredients in the formula are indispensable.

[0069] Table 1 Effects of different Chinese medicine compositions and Western medicines against H9N2 subtype AIV in chicken embryos

[0070]

[0071]

[0072] Note: Compared with the virus control group, the data in the same column with * indicates significant difference (P<0.05); compared with the western medicine control group, the data in the same column # Indicates significant difference (P<0.05); Compared with the Chinese medicine control group, the data in the same column + Indicates significant difference (P<0.05).

[0073] Test Example 2

[0074] 1 Materials and methods

[0075] 1.1 Experimental drugs:

[0076] (1) The Chinese medicine composition of the present invention is prepared into a Chinese medicine stock solution according to Example 8, with a concentration of 1 g / mL (each mL is equivalent to 1 g of the original medicinal material), and stored in a refrigerator at 0-4°C for future use;

[0077] (2) Qingwen Baidu San;

[0078] (3) 10% amoxicillin soluble powder.

[0079] 1.2 Experimental animals: 80 healthy 10-day-old Hy-Line brown laying hens

[0080] 1.3 Experimental design

[0081] 80 healthy Hailan brown laying hens aged 10 days were randomly divided into 4 groups, namely a blank control group, a Chinese medicine group of the present invention, a western medicine control group, and a Chinese medicine control group; on the basis of the original immunity, one dose of Newcastle disease avian influenza (H9 subtype) bivalent inactivated vaccine and 0.25 mL of avian influenza virus H5 subtype bivalent inactivated vaccine (Re-6 strain + Re-7 strain) were added. Blank control group: fed with normal saline; Chinese medicine group of the present invention: fed with the Chinese medicine stock solution prepared in Example 8, with a feeding dose of 0.2 mL / Kg; Western medicine control group: 0.5% amoxicillin aqueous solution by mass fraction; Chinese medicine control group: Qingwen Baidu powder, with a feeding dose of 0.2 g / Kg. Each group was given the drug according to the dose, and normal drinking water was fed for the rest of the time, and other feeding conditions and good environment were kept consistent. The drug was administered continuously for 14 days, followed by discontinuation of the drug, and normal feeding continued for 7 days. The total test period was 21 days. At 10 days, 17 days, 24 days, and 31 days of age, 10 laying hens were randomly selected from each group. Blood was collected from the wing vein of each chicken, 0.5 mL per chicken, and centrifuged after agglutination to separate the serum. The serum samples were tested for hemagglutination inhibition titer according to the method specified in the Technical Specifications for the Prevention and Control of Highly Pathogenic Avian Influenza, and statistical analysis was performed. When the chickens were raised to 31 days of age, all chickens were bled to death, and the bursa of Fabricius, thymus, and spleen were taken, the fat was removed, weighed, and recorded. The bursa of Fabricius, thymus, and spleen indexes were calculated.

[0082] Immune organ index (g / Kg) = immune organ weight (g) / animal weight (Kg)

[0083] 1.4 Data Analysis

[0084] The data were analyzed using Prism 8.0.2 statistical software, and all data were expressed as mean ± standard deviation.

[0085] 2 Results

[0086] 2.1 Effect of the Chinese medicine composition of the present invention on the antibody levels of Newcastle disease and avian influenza H5 subtype Re-6 and Re-7 strains

[0087] As shown in Table 2, before immunization, there was no significant difference in the antibody levels in the chickens of each group (P>0.05); at 17 days of age, the Newcastle disease antibody levels in the serum of the chickens in each drug-treated group increased significantly, and the difference was significant compared with the blank control group (P<0.05); at 24 days of age, the Newcastle disease antibody levels in the serum of the chickens in each drug-treated group reached the highest, among which the antibody level in the treatment group was the highest among the three groups, and was significantly different from the Western medicine control group and the Chinese medicine control group (P<0.05); at 31 days of age, the Newcastle disease antibody levels in the serum of the chickens in the Western medicine control group and the Chinese medicine control group decreased slightly compared with those at 17 days of age, and the difference was not significant (P>0.05); at 31 days of age, the Newcastle disease antibody levels in the serum of the chickens in the treatment group increased slightly compared with those at 17 days of age, and the difference was not significant (P>0.05).

[0088] Table 2 Effect of the Chinese medicine composition of the present invention on the level of Newcastle disease antibody in laying hen serum

[0089] Grouping 10 days old 17 days old 24 days old 31 days old Blank control group <![CDATA[3.95±0.30 Aa ]]> <![CDATA[3.91±0.33 Aa ]]> <![CDATA[3.90±0.24 Aa ]]> <![CDATA[3.94±0.21 Aa ]]> Western medicine control group <![CDATA[3.96±0.29 Aa ]]> <![CDATA[5.53±0.16 Bb ]]> <![CDATA[5.97±0.19 Cb ]]> <![CDATA[5.92±0.28 Cb ]]> Chinese medicine control group <![CDATA[3.95±0.25 Aa ]]> <![CDATA[4.96±0.17 Bc ]]> <![CDATA[5.51±0.23 Cc ]]> <![CDATA[5.41±0.19 Cc ]]> Treatment Group <![CDATA[3.88±0.30 Aa ]]> <![CDATA[5.58±0.18 Bb ]]> <![CDATA[6.37±0.21 Cd ]]> <![CDATA[6.42±0.23 Cd ]]>

[0090] Note: Different lowercase letters in the same column indicate significant differences (P<0.05), while the same or no lowercase letters indicate insignificant differences (P>0.05); Different uppercase letters in the same row indicate significant differences (P<0.05), while the same or no uppercase letters indicate insignificant differences (P>0.05), the same below.

[0091] As shown in Table 3, before immunization, there was no significant difference in the antibody levels in the chickens of each group (P>0.05); with the increase of age, the antibody levels of avian influenza H5 subtype Re-6 strain in the serum of laying hens in each experimental group showed a trend of first increasing and then decreasing; at 17 days of age, there was no significant difference between the western medicine control group and the Chinese medicine control group and the blank control group (P>0.05); there was a significant difference between the treatment group and the blank control group (P<0.05). At 24 days of age, there was no significant difference between the Chinese medicine control group and the blank control group (P>0.05); there was a significant difference between the western medicine control group and the treatment group and the blank control group (P<0.05); there was a significant difference between the treatment group and the western medicine control group (P<0.05). At 31 days of age, there was a significant difference between the western medicine control group and the Chinese medicine control group and the blank control group (P<0.05); there was a significant difference between the treatment group and the western medicine control group and the Chinese medicine control group (P<0.05). At 31 days of age, the antibody levels of each drug-treated group were lower than those at 24 days of age. Among them, the antibody levels of the western medicine control group and the traditional Chinese medicine control group at 31 days of age were significantly different from those at 24 days of age (P<0.05). There was no significant difference in the antibody levels of the treatment group at 31 days of age compared with those at 24 days of age (P>0.05).

[0092] Table 3 Effect of the Chinese medicine composition of the present invention on the antibody level of avian influenza H5 subtype Re-6 strain in laying hens serum

[0093] Grouping 10 days old 17 days old 24 days old 31 days old Blank control group <![CDATA[1.97±0.19 Aa ]]> <![CDATA[5.00±0.23 Ba ]]> <![CDATA[5.13±0.17 Ba ]]> <![CDATA[4.28±0.24 Ca ]]> Western medicine control group <![CDATA[1.99±0.21 Aa ]]> <![CDATA[5.02±0.31 Ba ]]> <![CDATA[5.36±0.22 Cb ]]> <![CDATA[4.85±0.21 Db ]]> Chinese medicine control group <![CDATA[1.90±0.24 Aa ]]> <![CDATA[5.01±0.23 Ba ]]> <![CDATA[5.12±0.15 Ba ]]> <![CDATA[4.66±0.15 Cb ]]> Treatment Group <![CDATA[1.93±0.16 Aa ]]> <![CDATA[5.92±0.23 Bb ]]> <![CDATA[6.35±0.32 Cc ]]> <![CDATA[6.12±0.18 Cc ]]>

[0094] As shown in Table 4, before immunization, there was no significant difference in the antibody levels in the chickens of each group (P>0.05); with the increase of age, the antibody levels of avian influenza H5 subtype Re-6 strain in the serum of laying hens in each experimental group showed a trend of first increasing and then decreasing; at 17 days of age, the western medicine control group and the Chinese medicine control group were significantly different from the blank control group (P<0.05); the treatment group was significantly different from the western medicine control group and the Chinese medicine control group (P<0.05). At 24 days of age, the western medicine control group, the Chinese medicine control group and the treatment group were significantly different from the blank control group (P<0.05); the treatment group was significantly different from the western medicine control group and the Chinese medicine control group (P<0.05). At 31 days of age, the western medicine control group, the Chinese medicine control group and the treatment group were significantly different from the blank control group (P<0.05); the treatment group was significantly different from the western medicine control group and the Chinese medicine control group (P<0.05). At 31 days of age, the antibody levels of each drug-treated group were lower than those at 24 days of age. Among them, the antibody levels of the western medicine control group and the traditional Chinese medicine control group at 31 days of age were significantly different from those at 24 days of age (P<0.05). There was no significant difference in the antibody levels of the treatment group at 31 days of age compared with those at 24 days of age (P>0.05).

[0095] Table 4 Effect of the Chinese medicine composition of the present invention on the antibody level of avian influenza H5 subtype Re-7 strain in laying hens' serum

[0096] Grouping 10 days old 17 days old 24 days old 31 days old Blank control group <![CDATA[2.28±0.20 Aa ]]> <![CDATA[5.01±0.17 Ba ]]> <![CDATA[5.20±0.18 Ba ]]> <![CDATA[4.78±0.18 Ca ]]> Western medicine control group <![CDATA[2.30±0.18 Aa ]]> <![CDATA[5.57±0.22 Bb ]]> <![CDATA[5.95±0.16 Cb ]]> <![CDATA[5.58±0.13 Db <!-- 7 -->]]> Chinese medicine control group <![CDATA[2.34±0.21 Aa ]]> <![CDATA[5.53±0.21 Bb ]]> <![CDATA[5.66±0.20 Bc ]]> <![CDATA[4.98±0.24 Cc ]]> Treatment Group <![CDATA[2.34±0.15 Aa ]]> <![CDATA[6.24±0.15 Bc ]]> <![CDATA[6.60±0.18 Cd ]]> <![CDATA[6.41±0.13 Cd ]]>

[0097] 2.2 Effects of the Chinese medicine composition of the present invention on the immune organs of laying hens

[0098] Figure 1 A-1C correspond to the bursal index, spleen index, and thymus index of the blank control group, western medicine control group, traditional Chinese medicine control group, and treatment group, respectively. Figure 1 It can be seen that the bursa index, spleen index and thymus index of the western medicine control group and the treatment group were significantly higher than those of the blank control group, with significant differences (P<0.05). The bursa index, spleen index and thymus index of the Chinese medicine group of the present invention were significantly higher than those of the western medicine control group and the Chinese medicine control group, with significant differences (P<0.05).

[0099] 3 Conclusion

[0100] From the above experimental results, it can be seen that the Chinese medicine composition of the present invention can significantly improve the antibody level of Newcastle disease and avian influenza H5 subtype Re-6 strain and Re-7 strain in laying hens, and can maintain the body's Newcastle disease and avian influenza H5 subtype Re-6 strain and Re-7 strain antibody titer high water for a long time, which is significantly better than the Western medicine control and the Chinese medicine control. At the same time, the Chinese medicine combination of the present invention has a positive effect on the immune organ index of laying hens, can significantly increase the immune organ index, and is better than the Western medicine control and the Chinese medicine control. The above results can illustrate that the Chinese medicine composition of the present invention has the effect of improving and maintaining the levels of various influenza antibodies, and by improving the body condition, the function of the immune organs is enhanced, and the Chinese medicine composition of the present invention can effectively promote the development of the body's immune organs, enhance the body's immune ability, and provide a material basis for the body to have a good resistance to antiviral infection.

[0101] Test Example 3

[0102] 30-50 days old laying hens diagnosed with avian influenza were selected. The clinical symptoms of the sick laying hens were: loss of appetite, depression, coughing, dyspnea, unwillingness to stand, decreased egg production, increased number of inferior eggs (broken shells, sand eggs, soft shells, dirty eggs, deformed eggs), etc. A total of 150 sick laying hens were randomly divided into 3 groups, namely a blank control group, a test group 1, and a test group 2, each with 50 hens. The blank control group was not medicated, fed normally, and fed freely and drank water; the test group 1 was administered with the Chinese medicine stock solution of the Chinese medicine composition of the present invention prepared in Example 8, with a dosage of 0.2 mL / Kg; the test group 2 was administered with morpholino hydrochloride injection, with a dosage of 1 mL / hen, twice a day; the test group 1 and the test group 2 were administered for 7 consecutive days, and the feeding conditions and environment of each group remained consistent.

[0103] Efficacy criteria:

[0104] (1) Effective: Mental state returned to normal, feed intake returned to normal, coughing and asthma symptoms were alleviated, egg production rate increased, and the number of poor quality eggs decreased;

[0105] (2) Cured: Good mental state, increased feed intake, no coughing and wheezing symptoms, willing to stand, egg production rate returns to normal levels, and the number of poor quality eggs is significantly reduced;

[0106] (3) Ineffective: Mental depression, no significant change in feed intake, coughing and wheezing symptoms have not disappeared, unwillingness to stand, egg production rate continues to decline, the number of low-quality eggs increases, and in severe cases, even death.

[0107] The therapeutic effect of the Chinese medicine composition of the present invention on laying hens suffering from avian influenza is shown in Table 5. The mortality rate of laying hens suffering from avian influenza without any treatment can be as high as 92%. After drug treatment, the clinical symptoms and signs of the sick laying hens in test group 1 and test group 2 were significantly improved and cured; the cure rate of the test group was 90.0%, and the effective rate was 98.0%, both higher than that of test group 2; and the ineffective rate of test group 1 was 2%, and the mortality rate was 0%, which was much lower than that of test group 2; there was a significant difference between test group 1 and test group 2. The above results show that the Chinese medicine composition of the present invention has a significant therapeutic effect on laying hens suffering from avian influenza, and the therapeutic effect is better than the use of antibiotics.

[0108] Table 5 The therapeutic effect of the Chinese medicine composition of the present invention on laying hens suffering from avian influenza

[0109] Grouping Healing (head / %) Effective (head / %) Invalid (header / %) Death (head / %) Blank control group — — — 46(92％) Test Group 1 45(90％) 49(98％) 1(2％) 0(0％) Test Group 2 37(74％) 42(84％) 8(16％) 2(4％)

[0110] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese medicine composition for treating avian influenza, characterized in that: The Chinese medicine composition is composed of the following Chinese medicine raw materials in parts by weight: 10-30 parts of dandelion, 10-30 parts of isatis indigotica, 5-15 parts of liquorice, 10-20 parts of forsythia, 5-15 parts of gardenia, 10-20 parts of rhubarb, 5-15 parts of sodium sulfate, 10-30 parts of atractylodes, 15-30 parts of scutellaria, 5-15 parts of anemarrhena, and 10-20 parts of calamus.

2. A Chinese medicine composition for treating avian influenza according to claim 1, characterized in that: The Chinese medicine composition is composed of the following Chinese medicine raw materials in parts by weight: 20-30 parts of dandelion, 10-20 parts of isatis indigotica, 8-12 parts of liquorice, 14-18 parts of forsythia, 5-10 parts of gardenia, 12-16 parts of rhubarb, 5-10 parts of sodium sulfate, 15-25 parts of atractylodes, 20-30 parts of scutellaria, 8-12 parts of anemarrhena, and 12-16 parts of calamus.

3. A Chinese medicine composition for treating avian influenza according to claim 1, wherein the Chinese medicine composition is composed of the following Chinese medicine raw materials in parts by weight: 26 parts of dandelions, 14 parts of isatis indigotica, 8 parts of liquorice, 15 parts of forsythia, 8 parts of gardenia, 15 parts of rhubarb, 8 parts of sodium sulfate, 22 parts of atractylodes, 26 parts of scutellaria, 10 parts of anemarrhena, and 14 parts of calamus.

4. The use of a Chinese medicine composition for treating avian influenza according to any one of claims 1 to 3, characterized in that: The Chinese medicine composition is used for preparing medicine for treating avian influenza, and the Chinese medicine composition is used for preparing any medicine dosage form acceptable to animals, and the dosage form is powder, granule, decoction, and oral liquid.

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