Application of Chinese herbal medicine Polygonum aviculare in the preparation of drugs for the prevention and treatment of stroke

Abstract

The present invention discloses the application of Chinese medicine Polygonum aviculare in the preparation of drugs for preventing and treating stroke. It relates to the field of biomedicine technology. The present invention shows that Chinese medicine Polygonum aviculare can significantly reduce the brain water content and brain index of experimental animals, and can treat brain edema. The drug has the effects of promoting blood circulation and removing blood stasis, can eliminate cerebral vascular blockage and inflammation, resist cerebral vasospasm, maintain normal cerebral vascular function, regulate normal permeability of brain barrier, promote angiogenesis, promote brain nerve function repair and regeneration, and greatly restore the nerve damage and behavioral ability of patients with cerebral stroke. Chinese medicine Polygonum aviculare can be dialectically used for the prevention and treatment of various courses of ischemic stroke and hemorrhagic stroke. The present invention not only provides a new application of Chinese medicine Polygonum aviculare, but also provides a new drug for preventing and treating ischemic stroke and hemorrhagic stroke.

Description

Technical Field

[0001] The present invention relates to the field of biomedicine technology, and more specifically, to the application of the traditional Chinese medicine Polygonum aviculare in the preparation of drugs for preventing and treating stroke. Background Art

[0002] "Cerebral stroke" is also called "stroke". It is an acute cerebrovascular disease. It is a group of diseases that cause brain tissue damage due to sudden rupture of brain blood vessels or blood vessel blockage that prevents blood from flowing into the brain. It includes ischemic stroke and hemorrhagic stroke. The incidence of ischemic stroke is higher than that of hemorrhagic stroke, accounting for 60% to 70% of the total number of strokes, and the mortality rate of hemorrhagic stroke is even higher. Stroke is characterized by high incidence, high mortality and high disability rate.

[0003] Traditional Chinese medicine has a good performance in dialectical treatment of two types of stroke. There are many reported traditional Chinese medicines for the treatment of stroke, such as the Chinese patent "A Chinese medicine compound preparation for the treatment of hemorrhagic stroke and its preparation method and application", "A pharmaceutical composition for the treatment of ischemic stroke", etc., but they only work on one type of stroke. In actual clinical practice, the two types of stroke often have many similarities. Therefore, exploring and developing a new traditional Chinese medicine product with definite efficacy for both ischemic and hemorrhagic stroke has important clinical value and practical significance.

[0004] Polygonum viscosum, also known as fragrant Polygonum, is the whole herb of the dicotyledonous plant Polygonum viscosum of the Polygonaceae family. Its main function is to dispel wind and dampness and is used for rheumatic arthralgia, but it has not yet been used in stroke. Summary of the invention

[0005] The purpose of the present invention is to provide a new Chinese medicine product for the treatment of stroke, which has good therapeutic effects on ischemic stroke and hemorrhagic stroke, that is, to provide the application of the Chinese medicine Polygonum aviculare in the preparation of drugs for preventing and treating stroke.

[0006] In order to achieve the above object, the present invention is implemented by the following scheme:

[0007] The invention provides application of the traditional Chinese medicine Polygonum aviculare in preparing a medicine for preventing and treating stroke.

[0008] Specifically, the stroke is ischemic stroke and / or hemorrhagic stroke.

[0009] The Chinese medicinal herb Polygonum aviculare is taken from the root, stem, leaf or whole plant of the original plant.

[0010] The medicine can reduce brain water content and brain index and treat brain edema.

[0011] The drug can maintain normal permeability of the brain barrier and promote the repair and regeneration of brain nerve function.

[0012] The drug can eliminate cerebral vascular blockage and inflammation, maintain normal cerebral vascular function, promote angiogenesis, and resist cerebral vascular spasm.

[0013] The drug can restore the neurological function and motor behavior of stroke patients.

[0014] Also provided is a medicine for preventing and treating stroke, which contains the traditional Chinese medicine Polygonum aviculare.

[0015] The drugs for preventing and treating stroke include ischemic stroke and hemorrhagic stroke.

[0016] The drug for preventing and treating stroke includes the acute phase, recovery phase and sequela phase of ischemic stroke.

[0017] The drug for preventing and treating stroke includes the acute phase, recovery phase and sequela phase of hemorrhagic stroke.

[0018] The medicine contains 10-200 g / day of the traditional Chinese medicine Polygonum aviculare (calculated as raw medicine).

[0019] The medicine contains 30-120 grams of the Chinese medicine Polygonum aviculare per day (calculated as raw medicine).

[0020] The drug may further include medically acceptable excipients or carriers.

[0021] The drug can be prepared into a variety of dosage forms, and representative dosage forms include but are not limited to decoctions, oral liquids, tablets, granules, capsules, pills, powders, emulsions, suppositories, injections and the like.

[0022] In order to better exert the effect of the Chinese medicine Polygonum aviculare in treating stroke, as an optional implementation scheme, the treatment method is: take the Chinese medicine Polygonum aviculare, wash it, add water and boil it, boil it 2-3 times, combine the decoctions, and concentrate the decoctions to obtain the Chinese medicine Polygonum aviculare decoction for treating stroke.

[0023] The root, stem, leaf or whole plant of the Chinese medicine Polygonum aviculare can be used as the raw material of the Chinese medicine Polygonum aviculare for treating stroke.

[0024] Among them, preferably, the Chinese medicine Polygonum aviculare: water: concentrated decoction = 90~120g: 250~400ml: 90~120ml.

[0025] In addition, the dosage form of the drug can be prepared into tablets, capsules, oral liquids, granules, pills, powders, emulsions, suppositories or injections, etc., in addition to decoctions.

[0026] As an optional implementation scheme, the preparation method of the Chinese medicine Polygonum glutinosa tablets is as follows: the above decoction is further concentrated and dried to obtain an extract, and an appropriate amount of medically acceptable auxiliary materials are added to prepare the Chinese medicine Polygonum glutinosa tablets.

[0027] As an optional implementation scheme, the preparation method of the Chinese medicine Polygonum multiflorum capsule is as follows: the above decoction is further concentrated and dried to obtain an extract, and an appropriate amount of medically acceptable auxiliary materials are added and mixed to prepare the Chinese medicine Polygonum multiflorum capsule.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] The present invention shows that the Chinese medicine Polygonum aviculare can significantly reduce brain water content and brain index, treat brain edema, eliminate brain inflammation, maintain normal brain barrier permeability, promote brain nerve function repair and regeneration, anti-oxidation, and promote angiogenesis. The medicine has the effects of promoting blood circulation and removing blood stasis, can eliminate cerebral vascular blockage and inflammation, resist cerebral vasospasm, maintain normal cerebral vascular function, regulate normal brain barrier permeability, promote angiogenesis, promote brain nerve function repair and regeneration, and greatly restore the neurological function and behavioral ability of stroke patients. The Chinese medicine Polygonum aviculare can be dialectically used for the treatment of ischemic stroke and hemorrhagic stroke. The present invention provides a new application of the Chinese medicine Polygonum aviculare, which provides a brand-new Chinese medicine product for the treatment of ischemic stroke and hemorrhagic stroke. DETAILED DESCRIPTION

[0030] The present invention is further described below with reference to specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0031] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0032] Example 1 Preparation of Chinese medicine Polygonum multiflorum decoction

[0033] Decoction: Take 100g of the dried whole plant of the Chinese medicinal plant Polygonum truncatum, rinse it clean, boil it with 300ml of water, boil it 3 times, combine the decoctions and concentrate it to 100ml to obtain the Chinese medicinal Polygonum truncatum decoction for treating stroke.

[0034] Example 2 Preparation of Chinese medicine Polygonum aviculare decoction

[0035] Decoction: Take 90g of dried Chinese medicinal plant Polygonum truncatum leaves, rinse them clean, and boil them with 250ml of water. Boil them twice, combine the decoctions and concentrate them to 90ml to obtain the Chinese medicinal plant Polygonum truncatum decoction for treating stroke.

[0036] Example 3 Preparation of Chinese medicine Polygonum aviculare decoction

[0037] Decoction: Take 120g of the dried root of the Chinese medicinal plant Polygonum glutinosa, rinse it clean, and boil it with 250ml of water. Boil it 3 times, combine the decoctions and concentrate it to 120ml to obtain the Chinese medicinal Polygonum glutinosa decoction for treating stroke.

[0038] Example 4 Preparation of Chinese medicine Polygonum multiflorum decoction

[0039] Decoction: Take 90g of the dried stems of the Chinese medicinal plant Polygonum glutinosa, rinse them clean, and boil them with 400ml of water. Boil them 3 times, combine the decoctions and concentrate them to 100ml to obtain the Chinese medicinal Polygonum glutinosa decoction for treating stroke.

[0040] Example 5 Preparation of Chinese medicine Polygonum multiflorum decoction

[0041] Decoction: Take 110g of the dried Chinese medicinal plant Polygonum truncatum root, rinse it clean, and boil it with 350ml of water. Boil it 3 times, combine the decoctions and concentrate it to 110ml to obtain the Chinese medicinal Polygonum truncatum decoction for treating stroke.

[0042] The following examples take the decoction prepared in Example 1 as an example to study the therapeutic effect and efficacy of the Chinese medicine Polygonum aviculare on stroke.

[0043] Example 6 Study on the Chinese medicine Polygonum aviculare for treating ischemic stroke

[0044] 1. Experimental methods

[0045] (1) Experimental groups and drug administration

[0046] SD rats were randomly divided into 7 groups, 3 rats in each group, namely: sham operation group, model group (model group was further divided into model group A, model group B, model group C), and drug administration group (drug administration group was further divided into drug administration group A, drug administration group B, and drug administration group C), wherein the model group and drug administration group were further divided into mild (A), moderate (B) and severe (C) neurological dysfunction groups according to the following modeling conditions and the results of the Garcia scoring method, mild refers to model group A and drug administration group A, moderate refers to model group B and drug administration group B, and severe refers to model group C and drug administration group C. The sham operation group was operated on and incised according to the following method, and the common carotid artery bifurcation, external carotid artery and internal carotid artery were separated and exposed, but no ligation and thread plug implantation were performed; the model group and drug administration group were implanted with thread plugs according to the following method, the difference was that the model group and the sham operation group were perfused with the same amount of physiological saline, and the drug administration group was perfused with 1.5g of the Chinese medicine Polygonum multiflorum decoction obtained in Example 1, and the perfusion times were once after the operation and once every 24 hours thereafter.

[0047] (2) Construction of rat ischemic stroke model

[0048] Several healthy male clean-grade SD (Sprague-Dawley) male rats (since modeling may not be successful and there is a possibility of death, the number of rats should be greater than the actual group size of 21) with a body weight of 280-300g were taken. After one week of adaptive feeding under the same conditions, the MCAO model was established. The feeding conditions were: 25±1℃, relative humidity of 50-60%, 12-hour light and dark alternation, and the same amount of drinking water and feed were provided. The light condition was a light intensity of 200lx. The suture method was used to construct a rat ischemic stroke model. The suture was used to implant and block the origin of the artery in the brain of SD rats, causing ischemia in the rat brain and focal cerebral ischemia, thereby constructing a rat ischemic stroke model. The specific model establishment method was as follows: first, a suture was prepared. A 27 mm 3-0 (No. 1) nylon suture was selected for the suture. The nylon suture was heated with an alcohol lamp to melt it into a hemispherical shape with a diameter of 0.33 ± 0.2 mm. After disinfection with alcohol, it was smeared with silicone grease under sterile conditions for use. Preparation: Rats were anesthetized by intraperitoneal injection of 40-60 mg / kg of 2% sodium pentobarbital. After anesthesia, the rats were fixed on the operating table in a supine position, and the body temperature of the rats was measured to ensure that the body temperature of the rats was normal and the rats were alive. The temperature was measured by using a rectal temperature probe to measure the rectal temperature of the rats; a scalpel was used to make a midline incision in the neck, and the thyroid connection was vertically bluntly separated with a vascular clamp to expose the neck muscles, and the muscle space composed of the digastric muscle, sternocleidomastoid muscle and omohyoid muscle was bluntly separated to gradually expose the bifurcation of the common carotid artery, the external carotid artery and the internal carotid artery. Then, the distal end of the external carotid artery and the proximal end of the common carotid artery were ligated with nylon sutures, and the internal carotid artery was pulled tightly with surgical thread to form a straight line with the common carotid artery. The internal carotid artery was clamped with an artery clamp, and a small cut was made at the common carotid artery with ophthalmic scissors. The above-mentioned hemispherical nylon suture with a diameter of 0.33 mm and coated with silicone was carefully inserted from the external carotid artery to the internal carotid artery until the beginning of the middle cerebral artery. The suture was inserted approximately 18±1 mm to block all blood supply sources of the middle cerebral artery on one side; then the wound was sutured, the excess nylon suture was cut off, and penicillin was used for anti-inflammatory treatment after the operation. After the rats woke up, the nerve injury score was calculated, and the Garcia score was used.

[0049] (3) Grouping based on Garcia score

[0050] The Garcia scoring method is used to screen and judge whether the model making is successful and to divide the groups. The lowest score of the Garcia scoring method is 3 points and the highest score is 18 points. The higher the score, the better the neurobehavioral condition of the animal. The first 4 items evaluate the motor function of the animal, and the last 2 items evaluate the sensory function of the animal. 3-7 are divided into severe neurological dysfunction, namely model group C and medication group C, 8-12 are divided into moderate neurological dysfunction, namely model group B and medication group B, and 13-16 are divided into mild neurological dysfunction, namely model group A and medication group A. Different rats are constructed with ischemic stroke models by inserting thread plugs. Due to the differences in the physiological state of each rat, the final results of neural damage are not the same, but after the damage is formed, the model score can be determined. Therefore, the model is grouped according to the Garcia score results to determine the grouping of rats with the same injury model. Garcia's scoring criteria are as follows: ① Autonomous movement (5 minutes of activity in the cage), no activity is scored as 0 points, almost no activity is scored as 1 point, activity is able to move but the range of activity does not reach the three sides of the cage is scored as 2 points, and the range of activity can reach at least 3 sides of the cage is scored as 3 points; ② Symmetry of limb activity, no activity on the left side is scored as 0 points, slight activity on the left side is scored as 1 point, slow activity on the left side is scored as 2 points, and symmetry of bilateral limb activity is scored as 3 points; ③ Extension and symmetry of the forelimbs when the tail is lifted, the left side is inactive and cannot be extended ④ The score was 0 for the left side to be able to move and slightly stretch the limbs, 1 for the left side to be able to move and stretch the limbs less than the right side, 2 for the left side to be less mobile and less stretchable than the right side, and 3 for the symmetrical extension of the bilateral forelimbs; ④ The climbing situation in the wire mouse cage was 1 for inability to climb, 2 for slightly weak left side, and 3 for normal climbing; ⑤ The reaction to touching the bilateral trunk was 1 for no reaction on the left side, 2 for weak reaction on the left side, and 3 for symmetrical reaction; ⑥ The reaction of the whiskers was 1 for no reaction on the left side, 2 for weakened reaction on the left side, and 3 for symmetrical reaction.

[0051] (4) Garcia score and brain-related data measurement

[0052] The Garcia scores of each group of experiments were recorded 7 days and 14 days after surgery and continuous drug treatment, as well as the change rate after 14 days; the rats were killed 14 days after drug treatment, and 50 ml / kg of Evans blue prepared with 2% saline was injected through the tail vein 2 hours before death to determine the permeability of brain vessels; after the rats were killed, the brain was removed by craniotomy, the brain weight was measured, and the values ​​of brain-related parameters were determined. Among them, the brain water content and the brain index are determined. The brain water content and the brain index can be used to determine whether the drug can work on brain edema caused by cerebral ischemia; the brain index is the ratio of 100 times the brain weight to the rat body weight, and the brain water content refers to the percentage of water in the brain to the wet weight of the brain; and the Evans blue content in the brain tissue is determined to verify that the Chinese medicine Polygonum aviculare can significantly reduce cerebral vascular permeability. The determination method is as follows: prepare a standard curve of Evans blue solution concentration and optical density, take a full-thickness brain tissue from the left and right sides of the anterior cerebral hemisphere, weigh it, add 3 ml of formamide, and after bathing at 37°C for 48 hours, take the brain Evans blue solution to determine the optical density (OD), the wavelength is 632 nm, distilled water is used as a blank for colorimetry, and the Evans blue solution content is calculated according to the standard curve.

[0053] 2. Experimental results

[0054] The experimental results are shown in Tables 1 and 2. From Table 1, it can be seen that the Garcia score increased significantly after the drug treatment. The drug has a greater restorative therapeutic effect on mild (dosage group A), moderate (dosage group B) and severe (dosage group C) neurological dysfunction groups. The administration of the drug can significantly improve the neurobehavioral status; from Table 2, the brain-related parameters of the drug group before and after the rat surgery model, brain water content and brain index were significantly lower than those of other groups, indicating that the drug can treat brain edema caused by ischemic stroke, and the Evans blue content in the drug group is lower than that in the model group, indicating that the drug can control the permeability of the brain barrier.

[0055] Table 1 Changes of Garcia scores after drug administration in rat ischemic stroke model

[0056]

[0057] Table 2 Brain-related parameters of rat ischemic stroke model after drug treatment and sacrifice

[0058]

[0059] Example 7 Study on the Treatment of Hemorrhagic Stroke with Chinese Medicine Polygonum multiflorum

[0060] 1. Experimental methods

[0061] (1) Experimental groups and drug administration

[0062] SD rats were randomly divided into 7 groups, 3 rats in each group, namely: sham operation group, model group (the model group was further divided into model group A, model group B, model group C), and drug administration group (the drug administration group was further divided into drug administration group A, drug administration group B, and drug administration group C). The model group and drug administration group were further divided into mild (A), moderate (B), and severe (C) neurological dysfunction groups according to the following modeling conditions and the results of the Garcia scoring method. Mild refers to model group A and drug administration group A, moderate refers to model group B and drug administration group B, and severe refers to model group C and drug administration group C. The sham operation group was operated on and incised according to the following method. The scalp was cut, the periosteum was removed, the anterior fontanelle was exposed, and the bleeding was fully stopped. A high-speed dental cranial drill was used to drill a hole (about 2 mm in diameter) on the skull until the dura mater was visible. The coordinates were: 0.2 mm in front of the anterior fontanelle, 3.5 mm lateral, and 5.5 mm deep. This location was the rat striatum area, but autologous arterial blood was not injected; the model group and the drug-treated group were implanted with thread plugs according to the following method. The difference was that the model group and the sham operation group were perfused with normal saline, and the drug-treated group was perfused with 1.5 g of the Chinese medicine Polygonum aviculare decoction obtained in Example 1. The perfusion times were once after the operation and once every 24 hours thereafter.

[0063] (2) Construction of a rat hemorrhagic stroke model

[0064] Take a number of healthy male clean-grade SD (Sprague-Dawley) male rats (since modeling may not be successful, there may be mortality, so the number of rats should be greater than the actual grouping size of 21), weighing 280-300g, and after one week of adaptive feeding under the same conditions, establish the hemorrhagic stroke model. The feeding conditions are: at a temperature of 25±1℃, a relative humidity of 50-60%, 12 hours of light and dark alternating cages, provide the same amount of drinking water and feed, and the light condition is a light intensity of 200lx. Use autologous blood injection to construct a rat hemorrhagic stroke model.

[0065] Rats were anesthetized by intraperitoneal injection of 40-60 mg / kg of 2% sodium pentobarbital. After anesthesia, the rats were fixed on the operating table in a supine position, and the body temperature of the rats was measured to ensure that the body temperature of the rats was normal and in a survival state. The temperature measurement method was to measure the rectal temperature of the rats with a rectal temperature probe. A scalpel was taken, and a midline incision was made in the neck. The scalp was cut, the periosteum was removed, and the anterior fontanelle was exposed. After hemostasis, a high-speed dental cranial drill was used to drill a hole in the rat's skull. The position was 0.2 mm in front of the anterior fontanelle and 3.5 mm to the side. The drilling diameter was about 2 mm, and the drilling depth was until the dura mater was visible. This part is the rat striatum area. After taking 100 μL of femoral artery blood with a syringe, it was immediately and slowly injected into the above-mentioned part at a speed of 20 μL / min. After staying for 20 min, it was slowly pulled out, and the bone hole was sealed with bone wax. The incision was carefully disinfected and sutured. To prevent sepsis, the entire experimental process was carried out in a sterile environment with constant temperature and sufficient light, and the animals were closely observed until they were fully awake.

[0066] (3) Determination of relevant data

[0067] The Garcia scores of each group of experiments were recorded after surgery and after 7 days and 14 days of continuous drug administration. The Garcia scores were the same as those in Example 1. The rats were killed 14 days after drug administration. 2 hours before the rats were killed, 50 ml / kg of Evans blue prepared with 2% saline was injected into the tail vein to measure the cerebral vascular permeability. After the rats were killed, the brains were removed by craniotomy, and the values ​​of brain-related parameters, brain water content and brain index were measured. The brain water content and brain index can be used to determine whether the drug can work on brain edema caused by cerebral ischemia. The brain index is the ratio of 100 times the brain weight to the rat body weight, and the brain water content refers to the percentage of brain water in the brain wet weight. The Evans blue content in the brain tissue was measured to verify that the Chinese medicine Polygonum aviculare can significantly reduce the cerebral vascular permeability. The determination method is as follows: a standard curve of Evans blue solution concentration and optical density was prepared, and a full-thickness brain tissue of the left and right sides of the anterior cerebral hemisphere was taken, and 3 ml of formamide was added after weighing, and the mixture was heated at 37 After 48 h in a water bath at ℃, the brain Evans blue solution was taken to measure the optical density (OD) at a wavelength of 632 nm. Distilled water was used as a blank for colorimetry, and the content of Evans blue solution was calculated according to the standard curve.

[0068] 2. Experimental results

[0069] The experimental results are shown in Tables 3 and 4. It can be seen from Table 3 that the Garcia score increased significantly after the drug treatment. This drug has a significant restorative therapeutic effect on the mild (dosage group A), moderate (dosage group B) and severe (dosage group C) neurological dysfunction groups.

[0070] Table 3 Changes of Garcia scores after drug treatment in rat hemorrhagic stroke model

[0071]

[0072] As shown in Table 4, the brain water content and brain index of the drug-treated group before and after the rat surgery model were significantly lower than those of other groups, indicating that the drug can treat brain edema caused by hemorrhagic stroke. The Evans blue content in the drug-treated group was lower than that in the model group, indicating that the drug can control the permeability of the brain barrier.

[0073] Table 4 Body weight changes before and after hemorrhagic stroke model in rats and brain-related parameters after sacrifice

[0074]

[0075] Example 8 Clinical application case

[0076] Stroke is divided into three stages: acute phase, recovery phase and sequela phase. The acute phase usually lasts less than 1 month after onset, the recovery phase lasts 2-6 months after onset, and the sequela phase lasts more than 6 months after onset. The Chinese medicine Polygonum aviculare has a definite effect in preventing and treating ischemic stroke and hemorrhagic stroke, and has been verified by a large number of clinical cases for more than 20 years. Representative cases are selected and displayed as follows:

[0077] 1. Case information and description of the condition

[0078] Case 1: Mr. Liu, male, 65 years old, had a cerebral infarction in 2005 and received medication for treatment. Symptoms before medication: unsteady standing, crooked mouth, drooling, unclear speech, and numbness of the head. The patient suffered from ischemic stroke and the course of the disease was in the sequelae stage.

[0079] Case 2: Ms. Li, female, 62 years old, suffered cerebral hemorrhage in 2007 for 3 years and was treated with medication. Symptoms before medication: unable to sit or stand, no feeling in feet. The patient suffered from hemorrhagic stroke and the course of the disease was in the sequelae stage.

[0080] Case 3: Mr. Deng, male, 71 years old, suffered a cerebral infarction in 2006, 11 years ago. He cannot walk and is in a wheelchair. The patient suffered from ischemic stroke and is in the sequelae stage.

[0081] Case 4: Male, 53 years old, suffered cerebral infarction nearly half a year in 2021 and has difficulty in moving. The patient has ischemic stroke and is in the recovery stage.

[0082] Case 5: Male, 72 years old, suffered cerebral infarction less than 5 months ago, needs assistance to walk. The patient suffered from ischemic stroke and is in the recovery stage.

[0083] Case 6: Female, 70 years old, suffered cerebral hemorrhage for nearly a month and is bedridden and unable to take care of herself. The patient suffered a hemorrhagic stroke and the disease is in the acute stage.

[0084] 2. Dosage

[0085] Case 1 Medication: 55±5 g of the decoction prepared by the method of Example 1 was taken for 21 days and then 13 days per year.

[0086] Case 2 Medication: 80±5 g of the decoction prepared by the method of Example 1 was taken for a total of 45 days.

[0087] Case 3 Medication: 55±5 g of the decoction prepared by the method of Example 1 was taken for a total of 26 days.

[0088] Case 4 Medication: 150±5g of the decoction prepared by the method of Example 1. When the amount of prepared medicine is insufficient, it can be decocted multiple times and the resulting decoctions can be mixed to ensure that the amount of raw herbs taken is sufficient. It is taken for a total of 15 days.

[0089] Case 5 Medication: 15±5 g of the decoction prepared by the method of Example 1 was taken for a total of 15 days.

[0090] Case 6 Medication: 85±5 g of the decoction prepared by the method of Example 1 was taken for a total of 21 days.

[0091] 3. Medication results

[0092] The medicine obtained according to the method of Example 1 is a decoction, and one dose of medicine is 10-200 grams. After treatment according to the above dosage, Case 1 to Case 6, Case 1 Liu took the medicine orally for 21 days, and took it for 13 days a year. He gradually got better every year, and now his body is normal and he has recovered. Case 2 Li took the medicine orally for 45 days, and the medicine took effect after 15 days. His feet recovered, his body could move, and he could sit up by himself; Case 3 Deng took the medicine for 13 days and was effective. He could walk 26 days later and could go from the first floor to the second floor; Case 4 was convenient to move after taking the medicine for 15 days, and his mobility was fully restored; Case 5 was significantly improved after taking the medicine for 15 days, and he could walk by himself; Case 6 took the medicine for 21 days, and his condition improved significantly, he could sit up, eat and brush his teeth by himself.

[0093] Example 9 Application Case Statistics

[0094] 1. Clinical trial data

[0095] The clinical efficacy of ischemic stroke was observed, including 30 cases in the acute stage, 35 cases in the recovery stage, and 68 cases in the sequelae stage. The Chinese medicine Polygonum multiflorum decoction was prepared according to the method of Example 1, with one dose of 10-200 grams, seven days as a course of treatment, to treat ischemic stroke patients respectively, and the drug efficacy was statistically analyzed, and the effective rate was calculated; the two groups of patients in the sequelae stage of ischemic stroke were treated with the above-mentioned drugs, and the ADL scores before and after treatment were statistically analyzed, and the average value was calculated; the clinical efficacy of hemorrhagic stroke was observed, including 18 cases in the acute stage, 28 cases in the recovery stage, and 45 cases in the sequelae stage, which were treated with the same drugs, the drug efficacy was statistically analyzed, and the effective rate was calculated; the two groups of patients in the sequelae stage of hemorrhagic stroke were treated with the above-mentioned drugs, and the ADL scores before and after treatment were statistically analyzed, and the average value was calculated.

[0096] 2. Efficacy evaluation criteria:

[0097] The evaluation of drug effects refers to the stroke diagnosis and efficacy evaluation standards and the clinical neurological deficit degree scoring standards for stroke patients. The clinical efficacy evaluation standards include: cured: conscious, language function recovery, muscle strength recovery of 3 or above or normal, able to engage in simple labor, and CT shows that the cerebral hemorrhage is completely absorbed. Significantly effective: language expression and limb function are significantly improved, muscle strength is improved by 2 or above, life is basically self-sufficient, and CT shows that the cerebral hemorrhage is basically absorbed. Effective: symptoms and signs are improved, muscle strength is improved by 1 or above, life can be partially self-sufficient, CT shows that the cerebral hemorrhage is not completely absorbed, and there is obvious cerebral edema. Ineffective: symptoms and signs are not significantly improved or aggravated, and CT shows no change or aggravation.

[0098] 3. Statistics of efficacy results

[0099] The efficacy results are shown in Tables 5 and 6. The Chinese medicine Polygonum aviculare can show significant clinical efficacy in all stages of ischemic stroke and hemorrhagic stroke, with an overall effectiveness of more than 83.3%. It can significantly improve and cure the sequelae of stroke and significantly improve the ADL scores of patients before and after treatment, with the ADL score improvement rate exceeding 117%.

[0100] Table 5 Observation of clinical efficacy in treating stroke

[0101]

[0102] Table 6 ADL scores of the two groups of patients with post-stroke sequelae before and after treatment

[0103]

[0104] The above-mentioned embodiments only express some implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several changes and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be based on the attached claims.

Claims

1. Application of a Chinese herbal medicine Polygonum viscidum extract in the preparation of a drug for preventing and treating stroke, wherein the Chinese herbal medicine Polygonum viscidum extract is obtained by decocting the Chinese herbal medicine Polygonum viscidum with water.

2. The use according to claim 1, characterized in that: The stroke is ischemic stroke and / or hemorrhagic stroke.

3. The use according to claim 1, characterized in that: The Chinese medicinal herb Polygonum aviculare is taken from the root, stem, leaf or whole plant of the original plant.

4. The use according to any one of claims 1 to 3, characterized in that: The drug can treat brain edema caused by stroke.

5. The use according to any one of claims 1 to 3, characterized in that: The drug can maintain normal permeability of the brain barrier and promote the repair and regeneration of brain nerve function.

6. The use according to any one of claims 1 to 3, characterized in that: The drug can eliminate cerebral vascular blockage and inflammation, maintain normal cerebral vascular function, promote angiogenesis, and resist cerebral vascular spasm.

7. The use according to any one of claims 1 to 3, characterized in that: The drug can restore the nerve damage and behavioral ability of stroke patients.

8. The use according to any one of claims 1 to 3, characterized in that: The dosage form of the stroke prevention and treatment medicine is decoction, tablet, capsule, oral liquid, granule, pill and powder.

9. The use according to claim 8, characterized in that: The dosage form of the drug for preventing and treating stroke is a decoction, and the preparation method thereof is as follows: taking the Chinese medicine Polygonum aviculare, adding water and boiling for 2-3 times, combining the decoctions and concentrating them, thus obtaining the Chinese medicine Polygonum aviculare decoction for treating stroke; wherein, the Chinese medicine Polygonum aviculare: water: concentrated decoction = 90-120 g: 250-400 ml: 90-120 ml.

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