Polysaccharide composition for improving skin immunity and consolidating skin barrier function and application thereof

Abstract

This invention belongs to the field of daily chemical products and discloses a polysaccharide composition that improves skin immunity and strengthens the skin barrier function, as well as its applications. The polysaccharide composition includes Scutellaria baicalensis polysaccharide, Adenophora stricta polysaccharide, and Trametes versicolor polysaccharide. Through the synergistic effect of these three polysaccharides, it can not only reduce the number of mast cells but also increase antimicrobial peptides and filaggrin; increase filaggrin; and decrease monocyte chemotactic protein-1 and interleukin-6, thereby achieving the effects of improving skin immunity, anti-inflammatory and whitening. Furthermore, it can act as a signaling molecule, widely participating in physiological processes such as intercellular ligand-receptor recognition and intercellular signal transduction, reducing the average relative amplification fold of interleukin-6 expression factor mRNA, and effectively participating in epidermal cell differentiation and skin barrier formation, thus strengthening the skin barrier function; it can be used in the preparation of cosmetics.

Description

[0001] This application is a divisional application of Chinese application No. 202111272768.0, filed on October 29, 2021, entitled "A polysaccharide composition that improves skin immunity and strengthens the skin barrier function and its application therein". Technical Field

[0002] This invention belongs to the field of daily chemical products, specifically relating to polysaccharide compositions that improve skin immunity and strengthen the skin barrier function, and their applications. Background Technology

[0003] Currently, there are many types of cosmetics on the market with varying effects, including moisturizing cosmetics, whitening cosmetics, and acne-removing cosmetics. However, their effects are often limited, lacking sufficient moisturizing and hydration, and failing to prevent skin aging, improve skin immunity, or strengthen the skin barrier. Furthermore, some whitening toners contain prohibited additives such as lead compounds and hydroquinone. Although they may whiten the skin in the short term, long-term use can lead to the accumulation of heavy metals, making the skin sensitive, irritating it, and causing localized pigmentation and a dull, yellowish complexion.

[0004] The quality of skin's moisturizing ability reflects the normal functioning of its skin barrier. Reduced skin moisturizing ability leads to a decline in skin barrier function, which in turn further reduces the skin's moisturizing ability, creating a vicious cycle. Therefore, moisturizing is the foundation and key to skincare.

[0005] Therefore, based on the needs of the cosmetics market, developing a polysaccharide composition that improves skin immunity and strengthens the skin barrier is of great significance for the research and development of whitening and moisturizing products. Summary of the Invention

[0006] The first aspect of the present invention is to provide a polysaccharide composition.

[0007] The second aspect of the present invention aims to provide the application of the polysaccharide composition of the first aspect.

[0008] The third aspect of this invention is to provide a product.

[0009] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0010] In a first aspect, the present invention provides a polysaccharide composition comprising at least three of the following: wolfberry polysaccharide, rose polysaccharide, Yunzhi polysaccharide, Scutellaria baicalensis polysaccharide, and Adenophora stricta polysaccharide.

[0011] Preferably, the polysaccharide composition comprises three of the following: wolfberry polysaccharide, rose polysaccharide, Yunzhi polysaccharide, Scutellaria baicalensis polysaccharide, and Adenophora stricta polysaccharide.

[0012] Preferably, the polysaccharide composition comprises any one of (1) to (5);

[0013] (1) Lycium barbarum polysaccharide, rose polysaccharide and Trametes versicolor polysaccharide;

[0014] (2) Scutellaria baicalensis polysaccharide, Adenophora stricta polysaccharide and Trametes versicolor polysaccharide;

[0015] (3) Polysaccharides from Adenophora stricta, Lycium barbarum, and Rose;

[0016] (4) Scutellaria baicalensis polysaccharide, Trametes versicolor polysaccharide and rose polysaccharide;

[0017] (5) Scutellaria baicalensis polysaccharide, Lycium barbarum polysaccharide and Adenophora stricta polysaccharide.

[0018] Preferably, the polysaccharide composition, by weight, comprises any one of (6) to (10);

[0019] (6) 0.5-1.5 parts of Lycium barbarum polysaccharide, 0.5-1.5 parts of Rose polysaccharide and 0.5-1.5 parts of Trametes versicolor polysaccharide;

[0020] (7) 0.5-1.5 parts of Scutellaria baicalensis polysaccharide, 0.5-1.5 parts of Adenophora stricta polysaccharide and 0.5-1.5 parts of Trametes versicolor polysaccharide;

[0021] (8) 0.5-1.5 parts of Nan Sha Shen polysaccharide, 0.5-1.5 parts of Goji Berry polysaccharide and 0.5-1.5 parts of Rose polysaccharide;

[0022] (9) 0.5-1.5 parts of Scutellaria baicalensis polysaccharide, 0.5-1.5 parts of Trametes versicolor polysaccharide and 0.5-1.5 parts of Rosa rugosa polysaccharide;

[0023] (10) 0.5-1.5 parts of Scutellaria baicalensis polysaccharide, 0.5-1.5 parts of Lycium barbarum polysaccharide and 0.5-1.5 parts of Adenophora stricta polysaccharide.

[0024] Preferably, the polysaccharide composition, by weight, comprises any one of (11) to (15);

[0025] (11) 1 part of wolfberry polysaccharide, 1 part of rose polysaccharide and 1 part of Yunzhi polysaccharide;

[0026] (12) 1 part of Scutellaria baicalensis polysaccharide, 1 part of Adenophora stricta polysaccharide and 1 part of Trametes versicolor polysaccharide;

[0027] (13) 1 part of Nansha ginseng polysaccharide, 1 part of wolfberry polysaccharide and 1 part of rose polysaccharide;

[0028] (14) 1 part of Scutellaria baicalensis polysaccharide, 1 part of Trametes versicolor polysaccharide and 1 part of Rosa rugosa polysaccharide;

[0029] (15) 1 part of Scutellaria baicalensis polysaccharide, 1 part of Lycium barbarum polysaccharide and 1 part of Adenophora stricta polysaccharide.

[0030] The polysaccharides of Adenophora stricta, Lycium barbarum, Rose, Trametes versicolor, and Scutellaria baicalensis can be commercially available or extracted from Adenophora stricta, Lycium barbarum, Rose, Trametes versicolor, and Scutellaria baicalensis, respectively.

[0031] Preferably, the rose is a rosebud.

[0032] Preferably, the extraction method is at least one of alcohol extraction and ultrasonic extraction; more preferably, it is alcohol extraction.

[0033] Preferably, the preparation method of the polysaccharide of Adenophora stricta is as follows: 1) defatting Adenophora stricta; 2) extracting polysaccharides from the defatted Adenophora stricta to obtain crude polysaccharide extract; 3) separating the crude polysaccharide extract into solid and liquid components to obtain an aqueous solution of Adenophora stricta polysaccharide, and drying it to obtain Adenophora stricta polysaccharide.

[0034] Preferably, the preparation method of the Nansha ginseng polysaccharide is as follows: Take dried Nansha ginseng, remove the parts with insects or deterioration, crush it, mix the crushed Nansha ginseng with a 95% ethanol solution at a material-to-liquid ratio of 1:10 (g:mL), let it stand overnight, filter to obtain a solid, add purified water (the material-to-liquid ratio of Nansha ginseng to purified water is 1:7.5 (g:mL)), heat at 90℃ for 3 h, filter with a 300-mesh filter bag to obtain a suspension, let it stand for 2 h, filter the suspension with double-layer medium-speed qualitative centrifuge paper to obtain an aqueous solution of Nansha ginseng polysaccharide, dry it to obtain Nansha ginseng polysaccharide.

[0035] Preferably, the preparation method of the wolfberry polysaccharide is as follows: 1) defatting wolfberry; 2) extracting polysaccharides from the defatted wolfberry to obtain crude polysaccharide extract; 3) separating the crude polysaccharide extract into solid and liquid components to obtain a wolfberry polysaccharide aqueous solution, which is then dried to obtain wolfberry polysaccharide.

[0036] Preferably, the preparation method of the wolfberry polysaccharide is as follows: take dried wolfberries, remove insect-infested or spoiled parts, crush them, mix the crushed wolfberries with a 95% ethanol solution at a material-to-liquid ratio of 1:10 (g:mL), let stand overnight, filter to obtain a solid, add purified water (the material-to-liquid ratio of wolfberries to purified water is 1:7.5 (g:mL)), heat at 90℃ for 3 h, filter with a 300-mesh filter bag to obtain a suspension, let stand for 2 h, filter the suspension with double-layer medium-speed qualitative centrifuge paper to obtain a wolfberry polysaccharide aqueous solution, dry to obtain wolfberry polysaccharide.

[0037] Preferably, the preparation method of the rose polysaccharide is as follows: 1) defatting the rose; 2) extracting polysaccharides from the defatted rose to obtain a crude polysaccharide extract; 3) separating the crude polysaccharide extract into solid and liquid components to obtain an aqueous solution of rose polysaccharide, which is then dried to obtain the rose polysaccharide.

[0038] Preferably, the preparation method of the rose polysaccharide is as follows: take dried rosebuds, remove insect-infested or deteriorated parts, crush them, mix the crushed roses with a 95% ethanol solution at a material-to-liquid ratio of 1:10 (g:mL), let stand overnight, filter to obtain a solid, add purified water (the material-to-liquid ratio of roses to purified water is 1:7.5 (g:mL)), heat at 90℃ for 3 h, filter with a 300-mesh filter bag to obtain a suspension, let stand for 2 h, filter the suspension with double-layer medium-speed qualitative centrifuge paper to obtain a rose polysaccharide aqueous solution, dry to obtain rose polysaccharide.

[0039] Preferably, the preparation method of the Coriolus versicolor polysaccharide is as follows: 1) defatting Coriolus versicolor; 2) extracting polysaccharides from the defatted Coriolus versicolor to obtain a crude polysaccharide extract; 3) separating the crude polysaccharide extract into solid and liquid components to obtain an aqueous solution of Coriolus versicolor polysaccharide, and drying it to obtain Coriolus versicolor polysaccharide.

[0040] Preferably, the preparation method of the Coriolus versicolor polysaccharide is as follows: take dried Coriolus versicolor, remove insect-infested or deteriorated parts, crush it, mix the crushed Coriolus versicolor with a 95% ethanol solution at a material-to-liquid ratio of 1:10 (g:mL), let it stand overnight, filter to obtain a solid, add purified water (the material-to-liquid ratio of Coriolus versicolor to purified water is 1:7.5 (g:mL)), heat at 90℃ for 3 h, filter with a 300-mesh filter bag to obtain a suspension, let it stand for 2 h, filter the suspension with double-layer medium-speed qualitative centrifuge paper to obtain an aqueous solution of Coriolus versicolor polysaccharide, dry it to obtain Coriolus versicolor polysaccharide.

[0041] Preferably, the preparation method of the Scutellaria baicalensis polysaccharide is as follows: 1) defatting Scutellaria baicalensis; 2) extracting polysaccharides from the defatted Scutellaria baicalensis to obtain crude polysaccharide extract; 3) separating the crude polysaccharide extract into solid and liquid components to obtain an aqueous solution of Scutellaria baicalensis polysaccharide, and drying it to obtain Scutellaria baicalensis polysaccharide.

[0042] Preferably, the preparation method of the Scutellaria baicalensis polysaccharide is as follows: take dried Scutellaria baicalensis, remove the parts with insects or deterioration, crush it, mix the crushed Scutellaria baicalensis with a 95% ethanol solution at a material-to-liquid ratio of 1:10 (g:mL), let it stand overnight, filter to obtain a solid, add purified water (the material-to-liquid ratio of Scutellaria baicalensis to purified water is 1:7.5 (g:mL)), heat at 90℃ for 3 h, filter with a 300-mesh filter bag to obtain a suspension, let it stand for 2 h, filter the suspension with double-layer medium-speed qualitative centrifuge paper to obtain an aqueous solution of Scutellaria baicalensis polysaccharide, dry it to obtain Scutellaria baicalensis polysaccharide.

[0043] In the polysaccharide composition of the present invention,

[0044] Southern ginseng, belonging to the Campanulaceae family, primarily possesses immune-regulating, antioxidant, and free radical scavenging properties. It can enhance skin resistance, whiten skin, and reduce collagen breakdown caused by sunlight. Southern ginseng is a polymer mainly composed of xylose, mannose, and glucuronic acid, which has the ability to scavenge hydroxyl free radicals, reduce lipofuscin content, and provides good moisturizing effects.

[0045] The main benefits of goji berries include anti-oxidation, moisturizing, and anti-aging. Therefore, they can moisturize and whiten the skin, and delay the aging process.

[0046] The main benefits of roses include anti-oxidation, anti-aging, and free radical scavenging. Therefore, they can moisturize and whiten the skin, and delay the aging process.

[0047] The main benefits of Ganoderma lucidum include strengthening the spleen and removing dampness, clearing heat and detoxifying. Therefore, it can moisturize and whiten the skin, delay aging, increase skin elasticity, and make the skin soft and delicate.

[0048] The main effects of Scutellaria baicalensis are to clear heat and dampness, and to stop bleeding. Therefore, it can keep the skin moisturized, whiten it, and delay aging.

[0049] The polysaccharide composition of the present invention improves skin immunity and strengthens the skin barrier through the synergistic effect of at least three components among Adenophora stricta polysaccharide, Lycium barbarum polysaccharide, Rose polysaccharide, Trametes versicolor polysaccharide and Scutellaria baicalensis polysaccharide, thereby enhancing the skin's whitening and moisturizing effects.

[0050] A second aspect of the invention provides the application of the polysaccharide composition of the first aspect of the invention.

[0051] Preferably, the polysaccharide composition of the first aspect of the present invention is used in any one of (a1) to (a7);

[0052] (a1) Preparation of cosmetics;

[0053] (a2) Prepare products with reduced skin thickness;

[0054] (a3) Prepare products that enhance the expression of antimicrobial peptides;

[0055] (a4) Prepare a product that reduces the expression level of monocyte chemoattractant protein-1 (MCP-1);

[0056] (a5) Prepare products that increase the expression level of filaggrin (FLG);

[0057] (a6) Prepare products that reduce the expression level of interleukin-6 (IL-6);

[0058] (a7) Prepare products that reduce the number of mast cells.

[0059] Preferably, the antimicrobial peptide is cathelicidin antimicrobial peptide (CAMP).

[0060] The cosmetics described in this invention are preparations made according to specific dosage form requirements. They primarily refer to products that, through application to the skin surface or other means, act on the body to improve skin immunity and strengthen the skin barrier function. These cosmetics can be gels, cleansers, toners, serums, creams, lotions, and moisturizers, but are not limited to these.

[0061] A third aspect of the present invention provides a product comprising the polysaccharide composition of the first aspect of the present invention.

[0062] Preferably, a cosmetic product comprises the polysaccharide composition and excipients of the first aspect of the present invention.

[0063] The excipients are at least one of the following: preservatives, chelating agents, fragrances, humectants, colorants, emulsifiers, antioxidants, and skin conditioning agents.

[0064] The cosmetics mentioned can be gels, facial cleansers, toners, serums, face creams, lotions, and moisturizers, but are not limited to these.

[0065] The beneficial effects of this invention are:

[0066] This invention provides a polysaccharide composition comprising at least three of the following: Lycium barbarum polysaccharide, Rose polysaccharide, Trametes versicolor polysaccharide, Scutellaria baicalensis polysaccharide, and Adenophora stricta polysaccharide. Through the synergistic effect of at least three of these components, it can not only reduce the number of mast cells but also increase antimicrobial peptide (cAMP), increase filaggrin (FLG), and decrease monocyte chemoattractant protein-1 (MCP-1) and interleukin-6 (IL-6), thereby improving skin immunity and achieving anti-inflammatory and whitening effects. Furthermore, it can act as a signaling molecule, widely participating in physiological processes such as intercellular ligand-receptor recognition and intercellular signal transduction, reducing the average relative amplification fold of interleukin-6 (IL-6) expression factor mRNA, and effectively participating in epidermal cell differentiation and skin barrier formation, thus strengthening the skin barrier function. It can be used in the preparation of cosmetics. Attached Figure Description

[0067] Figure 1 This is an image of the epidermal skin of mice after treatment with different polysaccharide compositions in Animal Experiment 1.

[0068] Figure 2 This is a graph showing the PASI score results of mice after being treated with different polysaccharide compositions in Animal Experiment 1.

[0069] Figure 3The figure shows the results of epidermal thickness after treating the back skin of mice with different polysaccharide compositions in Animal Experiment 1: where ### indicates "p < 0.05 compared with the blank group"; *** indicates "p < 0.05 compared with the model group".

[0070] Figure 4 The figure shows the results of epidermal thickness in mouse ear skin after treatment with different polysaccharide compositions in Animal Experiment 1: ### indicates "p < 0.05 compared with the blank group"; *** indicates "p < 0.05 compared with the model group".

[0071] Figure 5 The graph shows the results of antimicrobial peptide (cAMP) content in mice treated with different polysaccharide compositions in Animal Experiment 1: where ### indicates "p < 0.05 compared with the blank group"; *** indicates "p < 0.05 compared with the model group".

[0072] Figure 6 The graph shows the results of monocyte chemoattractant protein-1 (MCP-1) content in mice after treatment with different polysaccharide compositions in Animal Experiment 1: where ### indicates "p < 0.05 compared with the blank group"; *** indicates "p < 0.05 compared with the model group".

[0073] Figure 7 The graph shows the results of filamentous polymer (FLG) content in mice after treatment with different polysaccharide compositions in Animal Experiment 1: where ### indicates "p < 0.05 compared with the blank group"; *** indicates "p < 0.05 compared with the model group".

[0074] Figure 8 The graph shows the results of interleukin-6 (IL-6) content in mice after treatment with different polysaccharide compositions in Animal Experiment 1: where ### indicates "p < 0.05 compared with the blank group"; *** indicates "p < 0.05 compared with the model group".

[0075] Figure 9 This is a graph showing the results of interleukin-6 (IL-6) mRNA in mice after treatment with different polysaccharide compositions in Animal Experiment 1.

[0076] Figure 10 The images show the results of staining the back skin of mice treated with different polysaccharide compositions in Animal Experiment 1 using mast cell staining solution (toluidine blue method): A is a visual image of the back skin of mice treated with different polysaccharide compositions in Animal Experiment 1 using mast cell staining solution (toluidine blue method); B is a statistical result of mast cells after staining the back skin of mice treated with different polysaccharide compositions in Animal Experiment 1 using mast cell staining solution (toluidine blue method).

[0077] Figure 11This is an image of the epidermal skin of mice after treatment with different polysaccharide compositions in Animal Experiment 2.

[0078] Figure 12 This is a graph showing the PASI score results of mice after being treated with different polysaccharide compositions in Animal Experiment 2.

[0079] Figure 13 The images show the results of epidermal thickness after treatment of mouse dorsal skin with different polysaccharide compositions in Animal Experiment 2: A is a staining image of mouse dorsal skin after treatment with different polysaccharide compositions in Animal Experiment 2; B is a statistical result of epidermal thickness after treatment of mouse dorsal skin with different polysaccharide compositions in Animal Experiment 2; #### indicates "p < 0.05 compared with the blank group"; **** indicates "p < 0.05 compared with the model group".

[0080] Figure 14 The graph shows the results of antimicrobial peptide (cAMP) content in mice treated with different polysaccharide compositions in Animal Experiment 2: where ### indicates "p < 0.05 compared with the blank group"; *** indicates "p < 0.05 compared with the model group".

[0081] Figure 15 The figure shows the results of monocyte chemoattractant protein-1 (MCP-1) content in mice after treatment with different polysaccharide compositions in Animal Experiment 2: where ### indicates "p < 0.05 compared with the blank group"; *** indicates "p < 0.05 compared with the model group".

[0082] Figure 16 The graph shows the results of filamentous polymer (FLG) content in mice after treatment with different polysaccharide compositions in Animal Experiment 2: where ### indicates "p < 0.05 compared with the blank group"; *** indicates "p < 0.05 compared with the model group".

[0083] Figure 17 The graph shows the results of interleukin-6 (IL-6) content in mice after treatment with different polysaccharide compositions in Animal Experiment 2: where ### indicates "p < 0.05 compared with the blank group"; *** indicates "p < 0.05 compared with the model group".

[0084] Figure 18 The images show the results of staining mast cell staining (toluidine blue method) on the back skin of mice treated with different polysaccharide compositions in Animal Experiment 2. A is a visual representation of the mast cell staining (toluidine blue method) on the back skin of mice treated with different polysaccharide compositions in Animal Experiment 2; B is a statistical result of mast cells after staining (toluidine blue method) on the back skin of mice treated with different polysaccharide compositions in Animal Experiment 2; #### indicates "p < 0.05 compared to the control group"; **** indicates "p < 0.05 compared to the model group". Detailed Implementation

[0085] The present invention will be further described in detail below through specific embodiments.

[0086] It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the invention.

[0087] Experimental methods not specified in this embodiment are all conventional experimental methods. Experimental methods not specifying particular conditions in this embodiment are generally performed under conventional conditions or according to the manufacturer's recommendations. Unless otherwise specified, the materials and reagents used in this embodiment are commercially available, and the water may be deionized water, purified water, or distilled water.

[0088] The preparation method of Nan Sha Shen polysaccharide in this embodiment is as follows: Nan Sha Shen is ground into powder, 20 g is weighed and added to a blue-mouth bottle, and 200 mL of 95% ethanol solution is added. The bottle is sealed and left to stand overnight. The Nan Sha Shen-ethanol mixture after standing overnight is filtered, and 150 mL of purified water is added to the Nan Sha Shen filter. The mixture is then placed in a 90℃ constant temperature water bath and heated for 3 h. The Nan Sha Shen-purified water mixture is filtered through a 300-mesh filter bag to obtain a suspension. After standing for 2 h, the suspension is filtered through double-layer medium-speed qualitative centrifuge paper to obtain an aqueous solution of Nan Sha Shen polysaccharide. The solution is concentrated to about 50 mL. The Nan Sha Shen polysaccharide solution is shaken well, and 2 mL of the solution is taken with a pipette and placed in a centrifuge tube. The centrifuge tube is placed in a 70℃ oven to dry, and finally, Nan Sha Shen polysaccharide is obtained.

[0089] The preparation method of Lycium barbarum polysaccharide in this embodiment is as follows: Take Lycium barbarum powder, weigh 20 g and add it to a blue-mouthed bottle, along with 200 mL of 95% ethanol solution. Seal and let stand overnight. Filter the overnight Lycium barbarum-ethanol mixture, add 150 mL of purified water to the Lycium barbarum filter, and heat in a 90℃ constant temperature water bath for 3 h. Filter the Lycium barbarum-purified water mixture using a 300-mesh filter bag to obtain a suspension. After standing for 2 h, filter the suspension using double-layer medium-speed qualitative centrifuge paper to obtain an aqueous solution of Lycium barbarum polysaccharide. Concentrate the solution to about 50 mL, shake the Lycium barbarum polysaccharide solution well, and use a pipette to take 2 mL of the solution into a centrifuge tube. Place the centrifuge tube in a 70℃ oven to dry, finally obtaining Lycium barbarum polysaccharide.

[0090] The preparation method of rose polysaccharide in this embodiment is as follows: Take rose powder, weigh 20 g and add it to a blue-mouthed bottle, then add 200 mL of 95% ethanol solution, seal and let stand overnight; filter the overnight rose-ethanol mixture, add 150 mL of purified water to the rose filter, and heat in a 90℃ constant temperature water bath for 3 h; filter the rose-purified water mixture through a 300-mesh filter bag to obtain a suspension, let stand for 2 h, then filter the suspension through double-layer medium-speed qualitative centrifuge paper to obtain a rose polysaccharide aqueous solution, concentrate the solution to about 50 mL, shake the rose polysaccharide solution well, use a pipette to take 2 mL of the solution into a centrifuge tube, place the centrifuge tube in a 70℃ oven to dry, and finally obtain rose polysaccharide.

[0091] The preparation method of Coriolus versicolor polysaccharide in this embodiment is as follows: Coriolus versicolor powder is taken, 20 g is weighed and added to a blue-mouth bottle, and 200 mL of 95% ethanol solution is added. The bottle is sealed and left to stand overnight. The overnight Coriolus versicolor-ethanol mixture is filtered, 150 mL of purified water is added to the Coriolus versicolor filter, and the mixture is heated in a 90℃ constant temperature water bath for 3 h. The Coriolus versicolor-purified water mixture is filtered through a 300-mesh filter bag to obtain a suspension. After standing for 2 h, the suspension is filtered through double-layer medium-speed qualitative centrifuge paper to obtain an aqueous solution of Coriolus versicolor polysaccharide. The solution is concentrated to about 50 mL. The Coriolus versicolor polysaccharide solution is shaken well, and 2 mL of the solution is taken with a pipette and placed in a centrifuge tube. The centrifuge tube is placed in a 70℃ oven to dry, and finally Coriolus versicolor polysaccharide is obtained.

[0092] The preparation method of Scutellaria baicalensis polysaccharide in this embodiment is as follows: Scutellaria baicalensis is ground into powder, 20 g is weighed and added to a blue-mouth bottle, and 200 mL of 95% ethanol solution is added. The bottle is sealed and left to stand overnight. The overnight Scutellaria baicalensis-ethanol mixture is filtered, 150 mL of purified water is added to the Scutellaria baicalensis filter, and the mixture is heated in a 90℃ constant temperature water bath for 3 h. The Scutellaria baicalensis-purified water mixture is filtered through a 300-mesh filter bag to obtain a suspension. After standing for 2 h, the suspension is filtered through double-layer medium-speed qualitative centrifuge paper to obtain an aqueous solution of Scutellaria baicalensis polysaccharide. The solution is concentrated to about 50 mL. The Scutellaria baicalensis polysaccharide solution is shaken well, and 2 mL of the solution is taken into a centrifuge tube by pipette. The centrifuge tube is placed in a 70℃ oven to dry, and finally Scutellaria baicalensis polysaccharide is obtained.

[0093] Example 1: A polysaccharide composition that improves skin immunity and strengthens the skin barrier function (compound 1 group)

[0094] A polysaccharide composition that improves skin immunity and strengthens the skin barrier function comprises the following components in parts by weight: 1 part of wolfberry polysaccharide, 1 part of rose polysaccharide, and 1 part of Yunzhi polysaccharide.

[0095] Example 2: A polysaccharide composition (combined into two groups) that improves skin immunity and strengthens the skin barrier function.

[0096] A polysaccharide composition that improves skin immunity and strengthens the skin barrier function comprises the following components in parts by weight: 1 part of Scutellaria baicalensis polysaccharide, 1 part of Adenophora stricta polysaccharide, and 1 part of Trametes versicolor polysaccharide.

[0097] Example 3: A polysaccharide composition (combined into 3 groups) that improves skin immunity and strengthens the skin barrier function.

[0098] A polysaccharide composition that improves skin immunity and strengthens the skin barrier function comprises the following components in parts by weight: 1 part of Adenophora stricta polysaccharide, 1 part of Lycium barbarum polysaccharide, and 1 part of Rosa rugosa polysaccharide.

[0099] Example 4: A polysaccharide composition (4 formulations) that improves skin immunity and strengthens the skin barrier function.

[0100] A polysaccharide composition that improves skin immunity and strengthens the skin barrier function comprises the following components in parts by weight: 1 part of Coriolus versicolor polysaccharide, 1 part of rose polysaccharide, and 1 part of Scutellaria baicalensis polysaccharide.

[0101] Example 5: A polysaccharide composition that improves skin immunity and strengthens the skin barrier function (5 formulations)

[0102] A polysaccharide composition that improves skin immunity and strengthens the skin barrier function comprises the following components in parts by weight: 1 part of Lycium barbarum polysaccharide, 1 part of Adenophora stricta polysaccharide, and 1 part of Scutellaria baicalensis polysaccharide.

[0103] Comparative Example 1: A polysaccharide substance that improves skin immunity and strengthens the skin barrier function

[0104] A polysaccharide substance that improves skin immunity and strengthens the skin barrier function includes the following components by weight: 1 part rose polysaccharide.

[0105] Comparative Example 2: A polysaccharide substance that improves skin immunity and strengthens the skin barrier function

[0106] A polysaccharide substance that improves skin immunity and strengthens the skin barrier function includes the following components by weight: 1 part of Yunzhi polysaccharide.

[0107] Comparative Example 3: A polysaccharide substance that improves skin immunity and strengthens the skin barrier function

[0108] A polysaccharide substance that improves skin immunity and strengthens the skin barrier function includes the following components by weight: 1 part of wolfberry polysaccharide.

[0109] Comparative Example 4: A polysaccharide composition that improves skin immunity and strengthens the skin barrier function

[0110] A polysaccharide composition with improved skin immunity and consolidated skin barrier function, comprising the following components in parts by mass: 1 part of rose polysaccharide and 1 part of coriolus versicolor polysaccharide.

[0111] Comparative Example 5 A polysaccharide composition with improved skin immunity and consolidated skin barrier function

[0112] A polysaccharide composition with improved skin immunity and consolidated skin barrier function, comprising the following components in parts by mass: 1 part of wolfberry polysaccharide and 1 part of coriolus versicolor polysaccharide.

[0113] Comparative Example 6 A polysaccharide composition with improved skin immunity and consolidated skin barrier function

[0114] A polysaccharide composition with improved skin immunity and consolidated skin barrier function, comprising the following components in parts by mass: 1 part of wolfberry polysaccharide and 1 part of rose polysaccharide.

[0115] Application Example

[0116] An emulsion with improved skin immunity and consolidated skin barrier function, comprising the following components: 3.9 g of squalane, 2.1 g of Montanov S, 2.4 g of glycerol, 0.03 g of ethylparaben, 1.2 g of the polysaccharide composition in Example 1, and distilled water added to 30 g.

[0117] Effect Example 1

[0118] Respectively take the polysaccharide compositions / substances of Examples 1 to 5 and Comparative Examples 1 to 6 and dissolve them in propylene glycol to obtain a preparation with improved skin immunity and consolidated skin barrier function (the concentrations of wolfberry polysaccharide, rose polysaccharide, coriolus versicolor polysaccharide, adenophora tetraphylla polysaccharide, and scutellaria baicalensis polysaccharide in the preparation are all 0.5% (w / v)).

[0119] 1. Animal Experiment 1

[0120] Select a batch of BALB / c mice with normal morphology and similar body weights (animal batch number: SCXK (Guangdong) 2016-004). The BALB / c mice were adaptively raised for 7 days before the experiment; the back of the mice was depilated with sodium sulfide in nine mixtures for 2 cm * 4 cm, and the time of mouse depilation was D0; the following treatments were carried out respectively, and each treatment was repeated 10 times:

[0121] Blank group: Normal feeding. Starting from D8, apply 100 μL of propylene glycol to the depilated area on the back and 25 μL of propylene glycol to the right ear (inside and outside) every day for 9 days.

[0122] Model group: Starting on day 3, apply 100 μL of 0.15% (w / v) DNFB (prepared with acetone and olive oil at a volume ratio of 3:1) to the hair-removed area on the back, and apply 25 μL of 0.15% (w / v) DNFB (prepared with acetone and olive oil at a volume ratio of 3:1) to the right ear (inner and outer parts). Apply again on day 7, for a total of two applications. On day 10, stimulation begins, and 100 μL of 0.2% DNFB is applied to the back. Apply again on day 13, for a total of two applications, to establish an atopic dermatitis model. Starting on day 8, apply 100 μL of propylene glycol to the hair-removed area on the back, and apply 25 μL of propylene glycol to the right ear (inner and outer parts), once a day for a total of 9 days.

[0123] Positive group: Starting on day 3, apply 100 μL of 0.15% DNFB (prepared with acetone and olive oil at a volume ratio of 3:1) to the hair-removed area on the back, and apply 25 μL of 0.15% DNFB (w / v) (prepared with acetone and olive oil at a volume ratio of 3:1) to the right ear (inner and outer). Apply again on day 7, for a total of two applications. On day 10, stimulation begins, and 100 μL of 0.2% DNFB (w / v) is applied to the back. Apply again on day 13, for a total of two applications, to establish an atopic dermatitis model. Starting on day 8, apply 100 μL of 0.5% (w / v) prednisolone cream (Guangzhou Duoyang Biotechnology Co., Ltd.) to the hair-removed area on the back, and apply 25 μL of 0.5% prednisolone cream to the right ear (inner and outer), once a day for a total of 9 days.

[0124] In the treatment group: starting on day 3, 100 μL of 0.15% (w / v) DNFB (prepared with acetone and olive oil at a volume ratio of 3:1) was applied to the hair-removed area on the back, and 25 μL of 0.15% (w / v) DNFB (prepared with acetone and olive oil at a volume ratio of 3:1) was applied to the right ear (inner and outer parts). The application was repeated on day 7, for a total of two applications. On day 10, stimulation was initiated by applying 100 μL of 0.2% (w / v) DNFB to the back. The application was repeated on day 13, for a total of two applications, to establish an atopic dermatitis model. Starting on day 8, 100 μL of a preparation that improves skin immunity and strengthens skin barrier function (Examples 1-5 (combinations 1-5), Comparative Examples 1-6) was applied to the hair-removed area on the back, and 25 μL of a 0.5% (w / v) preparation that improves skin immunity and strengthens skin barrier function was applied to the right ear (inner and outer parts). The treatment was administered once daily for 9 days.

[0125] After D16 administration, blood was collected from the eyeballs of mice before euthanasia. The blood was allowed to stand at room temperature for 2 hours, then centrifuged at 1000g for 5 minutes at room temperature. The serum was collected and stored at -80℃. Mice were euthanized by cervical dislocation, and a 1cm sample was taken from the back skin of the experimental site. 2Divide into two parts, one part is fixed with 4% paraformaldehyde, and the other part is stored in a -80℃ refrigerator; take the left and right ears (non-test ears and test ears) respectively, make 8 mm diameter circular ear pieces, weigh and record the weight, and then fix with 4% paraformaldehyde. The inhibition rate, mouse dorsal skin condition, PASI score, thickness of the dorsal skin, thickness of the ear skin, antimicrobial peptide (CAMP), MCP-1, FLG, interleukin-6 (IL-6), number of mast cells stained with toluidine blue, and average relative fold increase of interleukin-6 (IL-6) expression factor mRNA were detected respectively (methods are described in the literature: Anti-allergic effects of novel sulfated polysaccharide sacran on mouse model of 2,4-Dinitro-1-fluorobenzene-induced atopic dermatitis, International Journal of Biological Macromolecules, Volume 108, March 2018, Pages 112-118).

[0126] The formula for calculating the inhibition rate is: Inhibition rate = (W DNFB -W 给药 ) / (W DNFB -W 空白 )*100%, where W DNFB The weight of the 8mm diameter round ear piece in the model group mice; W 给药 The weight of an 8mm diameter round ear piece in the treated mice; W 空白 The weight of an 8mm diameter round ear piece from the control group mice was used. The results are shown in Table 1: The polysaccharide composition provided in the examples, which improves skin immunity and strengthens the skin barrier function, can inhibit ear swelling in mice. The inhibition rate was ranked as follows: Compound 4 > Compound 5 > Positive > Compound 3 > Compound 1 > Compound 2, indicating that the polysaccharide composition provided in the examples, which improves skin immunity and strengthens the skin barrier function, can inhibit atopic dermatitis.

[0127] Table 1. Effect of polysaccharide compositions from different embodiments on inhibition rate.

[0128] Sort - Decreasing Group Inhibition rate (I, %) 1 Compound 4 132.4324 2 blank 100 3 Compound 5 73.51351 4 Positive 69.18919 5 Compound 3 68.10811 6 Compound 1 30.27027 7 Compound 2 21.62162 8 Model 0

[0129] The skin condition on the back of D16 mice is as follows: Figure 1 As shown: The polysaccharide composition provided in the example, which improves skin immunity and strengthens the skin barrier function, can inhibit atopic dermatitis. The inhibitory effect is as follows: Compound 4 > Compound 5 > Positive > Compound 3 > Compound 1 > Compound 2.

[0130] Starting from day 8, the daily changes in skin lesions of mice in each group were observed. The PASI scoring system was used to score the lesions on the backs of mice in each group daily. The PASI scoring system assigned scores of 0-4 to the degree of erythema, scales, and thickening at the lesion site: 0, none; 1, mild; 2, moderate; 3, severe; 4, very severe. The scores were summed to obtain a total score (0-12). The average scores for each group were then used to plot a trend line. The results are shown below. Figure 2 As shown: The polysaccharide composition provided in the example, which improves skin immunity and strengthens the skin barrier function, can reduce skin lesions in mice. The specific effects are as follows: positive > compound 4 > compound 5 > compound 2 > compound 1 > compound 3.

[0131] The results of the measurement of the epidermal layer thickness of the mouse dorsal skin are as follows: Figure 3 As shown: Compared with the model group, the polysaccharide compositions provided in Examples 1-3, which improve skin immunity and strengthen skin barrier function, can significantly reduce the thickness of the epidermal layer on the back of mice, and the effect is better than that of the positive group (prednisolone cream); in particular, the thickness reduction of the polysaccharide composition provided in Example 1 relative to the model group (0.81-0.38=0.43cm) is higher than the sum of the thickness reductions of each component (Comparative Examples 1-3) relative to the model group (0.13-0.07+0.2=0.26cm), indicating that the components in the polysaccharide composition provided in Example 1 have a synergistic effect in reducing the thickness of the epidermal layer on the back of the mouse.

[0132] The results of the measurement of the epidermal layer thickness of mouse ear skin are as follows: Figure 4 As shown: Compared with the model group, the polysaccharide compositions provided in Examples 1-5, which improve skin immunity and strengthen skin barrier function, can reduce the thickness of the epidermal layer of the mouse ear, and the effect is better than the positive group (prednisolone cream); among them, the polysaccharide compositions provided in Examples 1-3 can significantly reduce the thickness of the epidermal layer of the mouse ear.

[0133] The serum antimicrobial peptide (cAMP) content was detected using an ELISA kit (Jiangsu Enzyme Labeling Biotechnology MB-6350A) (see instruction manual for specific methods). The results are as follows: Figure 5As shown: Compared with the model group, the polysaccharide compositions provided in Examples 1-5 that improve skin immunity and strengthen skin barrier function can increase the content of antimicrobial peptides (CAMP) (among which, Examples 1-3 can significantly increase the content of antimicrobial peptides). In particular, the increase in the content of antimicrobial peptides (CAMP) relative to the model group by the polysaccharide composition provided in Example 1 (38.59-22.7=15.89ng / mL) is higher than the sum of the increases in the content of antimicrobial peptides (CAMP) relative to the model group by each component (Comparative Examples 1-3) and the sum of the increases in the content of antimicrobial peptides (CAMP) relative to the model group by a certain component (Comparative Examples 1-3) and the remaining components (Comparative Examples 4-6), indicating that the components in the polysaccharide composition provided in Example 1 have a synergistic effect in increasing the content of antimicrobial peptides (CAMP).

[0134] The serum monocyte chemoattractant protein-1 (MCP-1) level was detected using an ELISA kit (Jiangsu Enzyme Labeling Biotechnology MB-6161A) (see instruction manual for specific methods). The results are as follows: Figure 6 As shown: Compared with the model group, the polysaccharide compositions provided in Examples 1-5, which improve skin immunity and strengthen skin barrier function, can significantly reduce the content of monocyte chemoattractant protein-1 (MCP-1). In particular, the polysaccharide compositions provided in Examples 1-3 and 5 are more effective than the positive group (prednisolone cream).

[0135] The serum filaggrin (FLG) content was detected using an ELISA kit (Jiangsu Enzyme Labeling Biotechnology MB-5912A) (see instruction manual for specific methods). The results are as follows: Figure 7 As shown, compared with the model group, the polysaccharide compositions provided in Examples 1-5, which improve skin immunity and strengthen skin barrier function, can increase the content of filaggrin (FLG). Among them, the polysaccharide compositions provided in Examples 1 and 3-5 can significantly increase the content of filaggrin (FLG). In particular, the increase in the content of filaggrin (FLG) relative to the model group by the polysaccharide composition provided in Example 1 (10.27-5.52=4.75ng / mL) is higher than the sum of the increases in the content of each component (Comparative Examples 1-3) relative to the model group and the sum of the increases in the content of a certain component (Comparative Examples 1-3) and the remaining components (Comparative Examples 4-6) relative to the model group. This indicates that the components in the polysaccharide composition provided in Example 1 have a synergistic effect in increasing the content of filaggrin (FLG).

[0136] The serum interleukin-6 (IL-6) level was detected using an ELISA kit (Jiangsu Enzyme Labeling Biotechnology MB-2899A). The results are as follows: Figure 8Shown: Compared with the model group, the polysaccharide compositions provided in Examples 1-5, which have the effects of improving skin immunity and consolidating skin barrier function, can significantly reduce the content of interleukin-6 (IL-6), and the effect is better than that of the positive group (prednisolone cream).

[0137] Quantitative real-time fluorescence PCR was used to detect the content of interleukin-6 (IL-6) mRNA in serum: mRNA was extracted from mouse skin tissue using a total RNA extraction kit (purchased from Servicebio, catalog number: G3013), and the RNA content and purity met the requirements; reverse transcription kit was used to synthesize cDNA; GADPH was used as the internal reference gene, and the IL-6 primers were synthesized by Guangzhou Sevier Biotechnology Co., Ltd. (M-Il6-S: CCCC AAT TCC AAT GCT CTC C, SEQ ID NO.1; M-Il6-A: CGC ACT AGG TTT GCC GAG TA, SEQ ID NO.2). The mRNA expression levels of each group were detected according to the 2-ΔΔCt algorithm, and the results were as Figure 9 Shown: Compared with the model group, the polysaccharide compositions provided in Examples 1-5, which have the effects of improving skin immunity and consolidating skin barrier function, can reduce the expression level of interleukin-6 (IL-6) mRNA. In particular, the effects of the polysaccharide compositions provided in Examples 2, 4, and 5 are better than those of the positive group (prednisolone cream).

[0138] The back skin of mice was stained with mast cell staining solution (toluidine blue method), and the results were as Figure 10 Shown: Compared with the model group, the polysaccharide compositions provided in Examples 1-5, which have the effects of improving skin immunity and consolidating skin barrier function, can reduce the number of mast cells (the polysaccharide compositions provided in Examples 1 and 2 can significantly reduce the number). In particular, the effects of the polysaccharide compositions provided in Examples 1-3 and 5 are better than those of the positive group (prednisolone cream).

[0139] 2. Animal experiment 2

[0140] A batch of male BALB / c mice with normal morphology and similar body weights (animal batch number: SCXK (Yue) 2016-0041) was selected. After 7 days of adaptive feeding of the mice, the hair on the back of the mice was shaved about 2 cm * 2 cm, and 2% sodium sulfide was used for hair removal to make the villi fall off completely and the back was fully exposed. The hair removal of the mice was D0; the following treatments were carried out respectively, and each treatment was repeated 10 times:

[0141] Blank group: Normal feeding. Starting from D1, the blank group was infiltrated with a cotton ball moistened with water for 60 s. After 90 min of water treatment of the mice, 200 μL of propylene glycol was applied twice a day for 7 days.

[0142] Model group: Starting from day 1, mice were soaked in a 1:1 acetone-ether mixture for 30 seconds, followed by soaking in distilled water for 30 seconds. After treating the mice with the acetone-ether mixture for 90 minutes, 200 μL of propylene glycol was applied twice a day for 7 days.

[0143] Positive group: Starting on day 1, mice were soaked in a 1:1 acetone-ether mixture for 30 seconds, followed by soaking in distilled water for 30 seconds. After treating the mice with the acetone-ether mixture for 90 minutes, 200 μL of 0.5 (w / v)% hyaluronic acid was applied twice a day for 7 days.

[0144] In the treatment group: starting on day 1, mice were soaked in a 1:1 acetone-ether mixture for 30 seconds, followed by soaking in distilled water for 30 seconds. After treating the mice with the acetone-ether mixture for 90 minutes, 200 μL of a preparation that improves skin immunity and strengthens the skin barrier function (Examples 1-5 (compound 1-5), Comparative Examples 1-6) was applied twice a day for 7 days.

[0145] After the administration of medication on day 7, blood was collected from the eyeballs of mice before euthanasia. The blood was allowed to stand at room temperature for 2 hours, then centrifuged at 1000 g for 5 minutes at room temperature. The serum was collected and stored at -80°C. Mice were euthanized by cervical dislocation, and a 1 cm sample was taken from the back skin of the experimental site. 2 The sample was divided into two portions. One portion was fixed with 4% paraformaldehyde, and the other portion was stored at -80°C. The following parameters were measured: mouse dorsal skin condition; PASI score; dorsal skin epidermal thickness; antimicrobial peptide (cAMP); MCP-1; FLG; interleukin-6 (IL-6); number of mast cells stained with toluidine blue; and the average relative fold increase of interleukin-6 (IL-6) expression factor mRNA (methods described in the literature: Itch-Associated Response Induced by Experimental Dry Skin in Mice, Japanese Journal of Pharmacology, Volume 88, Issue 3, 2002, Pages 285-292).

[0146] The skin condition on the back of D7 mice is as follows: Figure 11 As shown: Compared with the model group, the polysaccharide compositions provided in Examples 3-5, which improve skin immunity and strengthen skin barrier function, can relieve symptoms such as dry skin and peeling, and the effect is better than that of the positive group.

[0147] Skin lesion changes in mice in each group were observed starting from day 7, and scored according to the PASI scoring criteria. The results are as follows: Figure 12As shown: The polysaccharide composition provided in the example, which improves skin immunity and strengthens the skin barrier function, can reduce skin lesions in mice. The specific effects are as follows: positive > compound 4 > compound 5 > compound 2 > compound 1 > compound 3.

[0148] The results of the measurement of the epidermal layer thickness of the mouse dorsal skin are as follows: Figure 13 As shown: Compared with the model group, the polysaccharide compositions provided in Examples 2-5, which improve skin immunity and strengthen skin barrier function, can significantly reduce the thickness of the epidermal layer on the back of mice, and the effect is better than that of the positive group.

[0149] The serum antimicrobial peptide (cAMP) content was detected using an ELISA kit (Jiangsu Enzyme Labeling Biotechnology MB-6350A) (see instruction manual for specific methods). The results are as follows: Figure 14 As shown, compared with the model group, the polysaccharide compositions provided in Examples 1-5, which improve skin immunity and strengthen skin barrier function, can increase the content of antimicrobial peptides (CAMP), and the effect is better than that of the positive group. Among them, the polysaccharide compositions provided in Examples 1-3, which improve skin immunity and strengthen skin barrier function, can significantly increase the content of antimicrobial peptides (CAMP). In particular, the increase in the content of antimicrobial peptides (CAMP) relative to the model group by the polysaccharide composition provided in Example 1 (43-18.51=24.49ng / mL) is higher than the sum of the increases in the content of antimicrobial peptides (CAMP) relative to the model group by each component (Comparative Examples 1-3) and the sum of the increases in the content of antimicrobial peptides (CAMP) relative to the model group by a certain component (Comparative Examples 1-3) and the remaining components (Comparative Examples 4-6), indicating that the components in the polysaccharide composition provided in Example 1 have a synergistic effect in increasing the content of antimicrobial peptides (CAMP).

[0150] The serum monocyte chemoattractant protein-1 (MCP-1) level was detected using an ELISA kit (Jiangsu Enzyme Labeling Biotechnology MB-6161A) (see instruction manual for specific methods). The results are as follows: Figure 15 As shown: Compared with the model group, the polysaccharide composition provided in Example 1, which improves skin immunity and strengthens skin barrier function, can reduce the content of monocyte chemoattractant protein-1 (MCP-1), and the effect is better than that of the positive group; while the components or combinations of two components in the polysaccharide composition provided in Example 1, which improves skin immunity and strengthens skin barrier function, all increase the content of monocyte chemoattractant protein-1 (MCP-1) compared with the model group, indicating that the components in the polysaccharide composition provided in Example 1 have achieved unexpected technical effects in reducing the content of monocyte chemoattractant protein-1 (MCP-1).

[0151] The serum filaggrin (FLG) content was detected using an ELISA kit (Jiangsu Enzyme Labeling Biotechnology MB-5912A) (see instruction manual for specific methods). The results are as follows: Figure 16 As shown, compared with the model group, the polysaccharide compositions provided in Examples 1-5, which improve skin immunity and strengthen skin barrier function, can increase the content of filaggrin (FLG). Among them, the polysaccharide compositions provided in Examples 1-3 can significantly increase the content of filaggrin (FLG). In particular, the increase in the content of filaggrin (FLG) relative to the model group by the polysaccharide composition provided in Example 1 (13.93-9.18=4.75ng / mL) is higher than the sum of the increases in the content of each component (Comparative Examples 1-3) relative to the model group, and the sum of the increases in the content of a certain component (Comparative Examples 1-3) and the remaining components (Comparative Examples 4-6) relative to the model group. This indicates that the components in the polysaccharide composition provided in Example 1 have a synergistic effect in increasing the content of filaggrin (FLG).

[0152] The serum interleukin-6 (IL-6) level was detected using an ELISA kit (Jiangsu Enzyme Labeling Biotechnology MB-2899A) (see the instruction manual for specific methods). The results are as follows: Figure 17 As shown: Compared with the model group, the polysaccharide compositions provided in Examples 1-5, which improve skin immunity and strengthen skin barrier function, can reduce the content of interleukin-6 (IL-6). Among them, the polysaccharide compositions provided in Examples 1-3 can significantly reduce the content of interleukin-6 (IL-6) and the effect is better than that of the positive group.

[0153] Mouse dorsal skin was stained using mast cell staining solution (toluidine blue method), and the results are as follows: Figure 18 As shown: Compared with the model group, the polysaccharide compositions provided in Examples 1-5, which improve skin immunity and strengthen skin barrier function, can significantly reduce the number of mast cells, and the effect is better than that of the positive group.

[0154] Nine indicators from the DNFB animal experiment (Animal Experiment 1) and seven indicators from the acetone-ethyl ether skin drying experiment (Animal Experiment 2) were weighted equally. The results of the two experimental indicators were ranked separately, with MCP-1, IL-6, back skin thickness, ear skin thickness, and inhibition rate in ascending order, and FLG, CAMP, skin score, and mast cell count in descending order. The rankings of each experimental data were summed to obtain a total weighted score. The lower the total weighted score, the better the comprehensive therapeutic effect of the plant polysaccharide combination on mice. The results are shown in Table 2.

[0155] Based on the total weighted score and ELISA results, the polysaccharide composition compound 1 was found to be more effective for skin symptoms than polysaccharide monomers and combinations of two polysaccharide monomers. Ultimately, compound 1, consisting of wolfberry polysaccharide, rose polysaccharide, and Yunzhi polysaccharide, was determined to be the most effective.

[0156] Table 2 Summary of results from Animal Experiment 1 and Animal Experiment 2

[0157]

[0158]

[0159] Example 2

[0160] 1. Human sensory testing

[0161] This test uses the emulsion prepared in the application examples and the excipients in the application examples (i.e., the combination of other components of the emulsion in the application examples except for the polysaccharide composition in Example 1) as controls to verify whether the product with the added polysaccharide composition in this example has significant moisturizing effects and skin-improving functions.

[0162] Experimental Methods: Before the experiment, 18 subjects aged 20-22 years with mixed-tolerance skin type and no allergies were selected. The test areas had not undergone any skin treatments, cosmetic procedures, or other tests that might affect the results. Measurement areas were marked on the outer forearms of both subjects, with a 1cm interval between the areas. For the arm test, two fixed 5cm x 5cm areas were selected on the front of the arm: one as the sample area (using the lotion from the example), and the other as the control sample area. The moisture content of the stratum corneum was measured every hour. The test conditions for this experiment were a temperature of 20-22℃ and a humidity of 50-55%.

[0163] The skin stratum corneum moisture content data are shown in Table 3. Higher skin stratum corneum moisture content indicates better moisturizing effect of the product. As can be seen from the data in Table 3, the control product has the lowest moisture content, indicating the worst moisturizing effect. The moisture content of the application example is significantly higher than that of the control product, proving its significant moisturizing effect. Therefore, the emulsion of the application example of this invention has significant moisturizing properties.

[0164] Table 3

[0165]

[0166] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. A polysaccharide composition, characterized in that, The polysaccharide composition, by weight, comprises 0.5-1.5 parts of Scutellaria baicalensis polysaccharide, 0.5-1.5 parts of Adenophora stricta polysaccharide, and 0.5-1.5 parts of Trametes versicolor polysaccharide; The polysaccharides of Adenophora stricta, Ganoderma lucidum, and Scutellaria baicalensis were extracted from Adenophora stricta, Ganoderma lucidum, and Scutellaria baicalensis, respectively. The preparation method of the polysaccharide of Adenophora stricta is as follows: 1) defatting Adenophora stricta; 2) extracting polysaccharides from the defatted Adenophora stricta to obtain crude polysaccharide extract; 3) separating the crude polysaccharide extract into solid and liquid components to obtain an aqueous solution of Adenophora stricta polysaccharide, drying it to obtain Adenophora stricta polysaccharide. The preparation method of Scutellaria baicalensis polysaccharide is as follows: 1) defatting Scutellaria baicalensis; 2) extracting polysaccharides from the defatted Scutellaria baicalensis to obtain crude polysaccharide extract; 3) separating the crude polysaccharide extract into solid and liquid components to obtain Scutellaria baicalensis polysaccharide aqueous solution, drying it to obtain Scutellaria baicalensis polysaccharide. The preparation method of the Coriolus versicolor polysaccharide is as follows: 1) defatting Coriolus versicolor; 2) extracting polysaccharides from the defatted Coriolus versicolor to obtain crude polysaccharide extract; 3) separating the crude polysaccharide extract into solid and liquid components to obtain an aqueous solution of Coriolus versicolor polysaccharide, which is then dried to obtain Coriolus versicolor polysaccharide.

2. The polysaccharide composition according to claim 1, characterized in that, The polysaccharide composition, by weight, consists of 1 part of Scutellaria baicalensis polysaccharide, 1 part of Adenophora stricta polysaccharide and 1 part of Ganoderma lucidum polysaccharide.

3. The use of the polysaccharide composition according to any one of claims 1-2 in the preparation of cosmetics.

4. The use of the polysaccharide composition according to any one of claims 1-2 in the preparation of products having the functions of inhibiting atopic dermatitis and / or strengthening the skin barrier.

5. The application according to claim 4, characterized in that, The product inhibits atopic dermatitis and / or strengthens the skin barrier through any one of (a1)-(a6); (a1) Reduce epidermal thickness; (a2) Increase the expression level of antimicrobial peptides; (a3) Reduce the expression level of monocyte chemoattractant protein-1; (a4) Increase the expression level of filaggrin; (a5) Reduces interleukin-6 expression; (a6) Reduce the number of mast cells.

6. A product comprising the polysaccharide composition according to any one of claims 1-2.

7. A cosmetic product comprising the polysaccharide composition and excipients as described in any one of claims 1-2; The excipients are at least one of the following: preservatives, chelating agents, fragrances, humectants, colorants, emulsifiers, antioxidants, and skin conditioning agents.

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