A high-pressure environment marine microorganism enrichment culture and gravity separation device

Abstract

The present invention discloses a high-pressure environment marine microorganism enrichment culture and gravity separation device, which belongs to the field of marine microorganism technology, and comprises: a culture chamber, the top of which is open; an upper cover, which is detachably mounted on the top of the culture chamber; a liquid storage cylinder, the bottom of which is rotatably connected with a curved pipe, the bottom end of which penetrates the upper cover and extends into the culture chamber, and a piston is installed in the liquid storage cylinder in a sliding seal, the top of the piston is hinged with two first connecting rods, and the two first connecting rods are hinged with a cross plate on one side close to each other. The present invention can drive the piston to move down along the liquid storage cylinder to gradually guide out the microorganism enrichment bacterial liquid through the cooperation of a screw rod, a synchronous pulley, a connecting plate and a mounting shaft, and can drive the curved pipe to rotate and rise at the same time under the cooperation of a gear, a bevel gear and a rack, so as to realize the spiral upward uniform delivery of the microorganism enrichment bacterial liquid, and can adjust the piston downward movement rate, so as to meet the needs of different output concentrations of the enrichment bacterial liquid.

Description

Technical Field

[0001] The invention relates to the technical field of marine microorganisms, and in particular to a high-pressure environment marine microorganism enrichment culture and gravity separation device. Background Art

[0002] Marine microbial resources are important strategic resources in the world, with the largest biodiversity. Technologies such as molecular ecology and metagenomics have significantly increased our understanding of marine microbial diversity. However, currently 99% of marine microorganisms have not been cultured in pure form, and many genes have unknown functions that are not annotated or may be incorrectly annotated in the database. Therefore, modern omics technology is not enough to provide enough information to understand all microorganisms, especially those that have not yet been classified. Improving the culturability of uncultured microorganisms and innovating pure culture methods are important ways to explain the phenotypes and genotypes of these microorganisms.

[0003] The patent document with publication number CN114350508B discloses a high-pressure environment marine microorganism enrichment culture and gravity separation device, including an enrichment and multi-level purification unit and a gravity separation culture unit; in a high-pressure, low-temperature environment consistent with the marine environment, the enrichment and multi-level purification unit is used to realize the enrichment and multi-level purification process of marine microorganisms, obtain the marine microorganism enriched bacterial liquid and inject the marine microorganism enriched bacterial liquid into the gravity separation culture unit; the gravity separation culture unit is used to use gravity to perform automatic streaking in a high-pressure environment.

[0004] In the above technical solution, the marine microorganism enriched bacterial liquid is diluted and dispersed in a concentration gradient as the small balls carrying the marine microorganism enriched bacterial liquid slide from top to bottom along the guide groove. The movement rate of the small balls during the dispersion process is uncontrollable, so that the dilution uniformity of the enriched bacterial liquid cannot be guaranteed. In addition, the input of the enriched bacterial liquid into the guide groove is determined by gravity, so that the output amount of the enriched bacterial liquid is fixed and cannot be adjusted according to needs. Therefore, we propose a high-pressure environment marine microorganism enrichment culture and gravity separation device to solve this problem. Summary of the invention

[0005] The object of the present invention is to provide a high-pressure environment marine microorganism enrichment culture and gravity separation device to solve the problems raised in the above background technology.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A high-pressure environment marine microorganism enrichment culture and gravity separation device, comprising:

[0008] A culture chamber, wherein the top of the culture chamber is open;

[0009] An upper cover, the upper cover is detachably mounted on the top of the culture chamber;

[0010] A liquid storage cylinder, wherein a bent tube is rotatably connected to the bottom of the liquid storage cylinder, the bottom end of the bent tube passes through the upper cover and extends into the culture chamber, and a piston is slidably and sealably installed in the liquid storage cylinder, the top of the piston is hinged with two first connecting rods, the sides of the two first connecting rods close to each other are hinged with a cross plate, the front and rear sides of the cross plate are rotatably connected to the second connecting rods, the bottom ends of the sides of the two second connecting rods close to each other are rotatably connected to the same side plate, the right side of the side plate is fixedly connected to a fixed plate, the fixed plate is fixedly connected to the left side of the liquid storage cylinder, the outer side of the cross plate is slidably sleeved with a sliding frame, the front and rear sides of the sliding frame are fixedly connected with a mounting shaft, the outer side of the mounting shaft is rotatably sleeved with a vertical plate, and the vertical plate is fixedly connected to the top of the upper cover;

[0011] A driving assembly, the driving assembly comprising three connecting plates, three screw rods and three synchronous pulleys, the same belt is installed on the three synchronous pulleys for transmission, and the three synchronous pulleys are respectively fixedly sleeved on the outside of the corresponding screw rods, the bottom end of the screw rod is rotatably connected to the top of the upper cover, the connecting plate is fixedly connected to the outside of the liquid storage cylinder, and the connecting plate is threadedly sleeved on the outside of the corresponding screw rod;

[0012] A linkage assembly, the linkage assembly includes a driven bevel gear, a driving bevel gear, a transmission gear and a rack, the rack is fixedly connected to the top of the upper cover, a horizontal axis is connected between the driving bevel gear and the transmission gear, the horizontal axis is rotatably installed at the bottom of the liquid storage cylinder, and the driven bevel gear is meshed with the driving bevel gear.

[0013] Preferably, two vertical plates are fixedly installed on the top of the horizontal plate, a screw rod is rotatably connected between the two vertical plates, a movable plate is fixedly installed on the top of the sliding frame, the movable plate is threadedly sleeved on the outside of the screw rod, and a knob is fixedly connected to the left end of the screw rod.

[0014] Preferably, a circular hole is provided on the top of the piston, a feed hopper is slidably installed in the circular hole, a sealing plate is fixedly installed on the bottom of the feed hopper, the sealing plate movably abuts against the bottom of the piston, and a circular ring is fixedly installed on the outside of the feed hopper, a support spring is fixedly connected to the bottom of the circular ring, and the bottom ends of multiple support springs are fixedly connected to the top of the piston.

[0015] Preferably, a motor seat is fixedly installed on the front side of the vertical plate located at the rear side of the two vertical plates, a driving motor is fixedly installed on the bottom of the motor seat, and the output shaft of the driving motor is fixedly connected to the top end of the corresponding screw rod.

[0016] Preferably, two mounting plates and a plurality of vertical rods are fixedly installed on the bottom of the liquid storage cylinder, and the two mounting plates are rotatably sleeved on the outside of the horizontal axis, the bottom ends of the vertical rods are fixedly connected to a limiting frame, the outside of the bent pipe is fixedly sleeved with a limiting ring, and the limiting frame movably abuts against the outside of the limiting ring.

[0017] Preferably, a square groove is provided on the top of the horizontal plate, a convex plate is slidably installed in the square groove, a plurality of positioning grooves are provided on the outer side of the knob, the convex plate is movably engaged in the corresponding positioning grooves, and the bottom plate of the convex plate is fixedly connected with a pull rod and a compression spring, and the bottom end of the compression spring is fixedly connected to the bottom inner wall of the corresponding square groove.

[0018] Preferably, buckling plates are fixedly installed on both sides of the top of the culture chamber, and the buckling plates are movably abutted against the outer side of the upper cover, and arc-shaped protrusions are integrally formed on both sides of the upper cover, and the two buckling plates are movably abutted against the tops of the corresponding arc-shaped protrusions, and vertical holes are provided on both sides of the top of the culture chamber, and card plates are slidably installed in the vertical holes, and card grooves are provided on both sides of the bottom of the upper cover, and the two card plates are movably clamped in the corresponding card grooves, and the bottoms of the two card plates are fixedly connected to the same annular plate, the annular plate is slidably sleeved on the outside of the culture chamber, and a plurality of connecting springs are fixedly connected to the bottom of the annular plate, and a fixing ring is fixedly sleeved on the outside of the culture chamber, and the bottom end of the connecting spring is fixedly connected to the top of the fixing ring.

[0019] Preferably, three guide columns are fixedly connected to the top of the upper cover, and the three connecting plates are respectively slidably sleeved on the outer sides of the corresponding guide columns, and a positioning plate is fixedly installed on the top of the guide column, and the three positioning plates are respectively rotatably sleeved on the outer sides of the corresponding screw rods.

[0020] Preferably, a plurality of guide rods are fixedly mounted on the top of the piston, and the ring is slidably sleeved on the outer sides of the guide rods.

[0021] Preferably, a U-shaped rod is fixedly installed on the left side of the top of the piston, and the fixing plate is slidably sleeved on the outer side of the U-shaped rod.

[0022] The beneficial effects of the present invention are:

[0023] 1. In the present invention, the high-pressure environment marine microorganism enrichment culture and gravity separation device is used to fix the upper cover by the cooperation of the buckle plate and the card plate, and the high-pressure and extreme temperature environment conditions for the microorganisms to live in the marine environment are constructed by the cooperation of the upper cover and the culture chamber;

[0024] 2. In the present invention, the high-pressure environment marine microorganism enrichment culture and gravity separation device can drive the piston to move down along the liquid storage cylinder to gradually discharge the microorganism enriched bacterial liquid through the cooperation of the screw rod, synchronous pulley, connecting plate, cross plate, first connecting rod, second connecting rod and mounting shaft, and can drive the curved pipe to rotate and rise at the same time under the cooperation of gear, bevel gear and rack, so as to realize the spiral upward uniform delivery of the microorganism enriched bacterial liquid;

[0025] 3. In the present invention, the high-pressure environment marine microorganism enrichment culture and gravity separation device can adjust the speed of the piston moving downward through the cooperation of the convex plate, the knob, the screw and the slide frame, so as to meet the needs of different output concentrations of the enriched bacterial liquid;

[0026] 4. In the present invention, the high-pressure environment marine microorganism enrichment culture and gravity separation device can conveniently add the microorganism enrichment liquid into the liquid storage cylinder through the cooperation of the feed hopper, the sealing plate, the support spring and the piston, and can also realize the automatic sealing of the liquid storage cylinder;

[0027] 5. In the present invention, the high-pressure environment marine microorganism enrichment culture and gravity separation device can ensure that the bent pipe can rotate and follow the liquid storage cylinder to perform stable lifting and lowering work through the cooperation of the limit frame and the limit ring, resulting in the effect of microorganism enrichment and bacterial liquid culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of the three-dimensional structure of a high-pressure environment marine microorganism enrichment culture and gravity separation device proposed by the present invention;

[0029] Figure 2 This is a schematic diagram of the front cross-sectional structure of a high-pressure environment marine microorganism enrichment culture and gravity separation device proposed by the present invention;

[0030] Figure 3 for Figure 2 A partial enlarged view of part A;

[0031] Figure 4 for Figure 2 A partial enlarged view of part B;

[0032] Figure 5 for Figure 2 A partial enlarged view of part C in the middle;

[0033] Figure 6 for Figure 2 A partial enlarged view of part D in the middle;

[0034] Figure 7 This is a top view and cross-sectional structure schematic diagram of a high-pressure environment marine microorganism enrichment culture and gravity separation device proposed by the present invention;

[0035] Figure 8 This is a schematic diagram of the partial three-dimensional structure of a high-pressure environment marine microorganism enrichment culture and gravity separation device proposed by the present invention;

[0036] Fig. 9 for Figure 8 A partial enlarged view of part E in the middle;

[0037] Fig.10 This is a schematic diagram of the three-dimensional structure of the feed hopper and sealing plate proposed by the present invention.

[0038] In the figure: 1, culture chamber; 2, upper cover; 3, liquid storage cylinder; 4, buckle plate; 5, screw rod; 6, piston; 7, first connecting rod; 8, driving motor; 9, slide frame; 10, horizontal plate; 11, mounting shaft; 12, second connecting rod; 13, feed hopper; 14, vertical plate; 15, convex plate; 16, fixed plate; 17, U-shaped rod; 18, side plate; 19, pull rod; 20, compression spring; 21, knob; 22, screw rod; 2 3. Moving plate; 24. Positioning plate; 25. Guide column; 26. Sealing plate; 27. Circular ring; 28. Support spring; 29. ​​Rack; 30. Bend pipe; 31. Connecting plate; 32. Clamping plate; 33. Synchronous pulley; 34. Driven bevel gear; 35. Driving bevel gear; 36. Transmission gear; 37. Horizontal axis; 38. Vertical plate; 39. Limiting frame; 40. Limiting ring; 41. Annular plate; 42. Connecting spring. DETAILED DESCRIPTION

[0039] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0040] Reference Figure 1-10 , a high-pressure environment marine microorganism enrichment culture and gravity separation device, comprising:

[0041] The top of the culture room 1 is open;

[0042] An upper cover 2, which is detachably mounted on the top of the culture chamber 1;

[0043] A liquid storage cylinder 3, the bottom of which is rotatably connected with a curved tube 30, the bottom end of which passes through the upper cover 2 and extends into the culture chamber 1, and a piston 6 is slidably and sealably installed in the liquid storage cylinder 3, the top of the piston 6 is hinged with two first connecting rods 7, the sides of the two first connecting rods 7 close to each other are hinged with a transverse plate 10, the front and rear sides of the transverse plate 10 are rotatably connected with second connecting rods 12, the bottom ends of the sides of the two second connecting rods close to each other are rotatably connected with the same side plate 18, the right side of the side plate 18 is fixedly connected with a fixed plate 16, the fixed plate 16 is fixedly connected to the left side of the liquid storage cylinder 3, the outer side of the transverse plate 10 is slidably sleeved with a sliding frame 9, the front and rear sides of the sliding frame 9 are fixedly connected with a mounting shaft 11, the outer side of the mounting shaft 11 is rotatably sleeved with a vertical plate 14, and the vertical plate 14 is fixedly connected to the top of the upper cover 2;

[0044] The driving assembly includes three connecting plates 31, three screw rods 5 and three synchronous pulleys 33. The same belt is installed on the three synchronous pulleys 33 for transmission, and the three synchronous pulleys 33 are respectively fixedly sleeved on the outer sides of the corresponding screw rods 5. The bottom ends of the screw rods 5 are rotatably connected to the top of the upper cover 2. The connecting plates 31 are fixedly connected to the outer sides of the liquid storage cylinder 3, and the connecting plates 31 are threadedly sleeved on the outer sides of the corresponding screw rods 5.

[0045] The linkage assembly includes a driven bevel gear 34, a driving bevel gear 35, a transmission gear 36 and a rack 29. The rack 29 is fixedly connected to the top of the upper cover 2. A horizontal shaft 37 is connected between the driving bevel gear 35 and the transmission gear 36. The horizontal shaft 37 is rotatably installed at the bottom of the liquid storage cylinder 3. The driven bevel gear 34 is meshed with the driving bevel gear 35.

[0046] In this embodiment, two vertical plates 38 are fixedly installed on the top of the horizontal plate 10, and a screw 22 is rotatably connected between the two vertical plates 38. A movable plate 23 is fixedly installed on the top of the sliding frame 9. The movable plate 23 is threadedly sleeved on the outer side of the screw 22, and the left end of the screw 22 is fixedly connected with a knob 21, so as to facilitate the adjustment of the lateral position of the horizontal plate 10. A square groove is provided on the top of the horizontal plate 10, and a convex plate 15 is slidably installed in the square groove. A plurality of positioning grooves are provided on the outer side of the knob 21, and the convex plate 15 is movably clamped in the corresponding positioning grooves, and the bottom plate of the convex plate 15 is fixedly connected with a pull rod 19 and a compression spring 20, and the bottom end of the compression spring 20 is fixedly connected to the bottom inner wall of the corresponding square groove, thereby improving the locking effect of the screw 22.

[0047] In this embodiment, a circular hole is provided on the top of the piston 6, and a feed hopper 13 is slidably installed in the circular hole, so as to facilitate the addition of microbial enriched bacterial liquid into the liquid storage cylinder 3. A sealing plate 26 is fixedly installed on the bottom of the feed hopper 13, and the sealing plate 26 is movably abutted against the bottom of the piston 6, thereby sealing the piston 6, and a circular ring 27 is fixedly installed on the outer side of the feed hopper 13, and a support spring 28 is fixedly connected to the bottom of the circular ring 27, and the bottom ends of multiple support springs 28 are fixedly connected to the top of the piston 6, thereby supporting the feed hopper 13.

[0048] In this embodiment, a motor seat is fixedly installed on the front side of the rear vertical plate 14 of the two vertical plates 14, and a driving motor 8 is fixedly installed on the bottom of the motor seat. The output shaft of the driving motor 8 is fixedly connected to the top end of the corresponding screw rod 5, thereby facilitating the driving screw rod 5 to rotate.

[0049] In this embodiment, two mounting plates and a plurality of vertical rods are fixedly installed at the bottom of the liquid storage cylinder 3. Both mounting plates are rotatably sleeved on the outside of the horizontal axis 37. The bottom ends of the vertical rods are fixedly connected to a limiting frame 39. A limiting ring 40 is fixedly sleeved on the outside of the curved pipe 30. The limiting frame 39 is movably abutted against the outside of the limiting ring 40, thereby limiting the vertical movement of the curved pipe 30.

[0050] In this embodiment, buckling plates 4 are fixedly installed on both sides of the top of the culture chamber 1, and the buckling plates 4 are movably abutted against the outer side of the upper cover 2, and arc-shaped protrusions are integrally formed on both sides of the upper cover 2. The two buckling plates 4 are movably abutted against the tops of the corresponding arc-shaped protrusions, and vertical holes are opened on both sides of the top of the culture chamber 1, and card plates 32 are slidably installed in the vertical holes. Card grooves are opened on both sides of the bottom of the upper cover 2, and the two card plates 32 are movably clamped in the corresponding card grooves, thereby realizing the fixation of the culture chamber 1 and the upper cover 2, and the bottoms of the two card plates 32 are fixedly connected with the same annular plate 41, and the annular plate 41 is slidably sleeved on the outer side of the culture chamber 1, and the bottom of the annular plate 41 is fixedly connected with a plurality of connecting springs 42, and a fixing ring is fixedly sleeved on the outer side of the culture chamber 1, and the bottom end of the connecting spring 42 is fixedly connected to the top of the fixing ring, thereby supporting and resetting the annular plate 41.

[0051] In this embodiment, three guide columns 25 are fixedly connected to the top of the upper cover 2, and three connecting plates 31 are slidably sleeved on the outer sides of the corresponding guide columns 25, thereby guiding the connecting plates 31, and a positioning plate 24 is fixedly installed on the top of the guide column 25, and the three positioning plates 24 are rotatably sleeved on the outer sides of the corresponding screw rods 5, thereby rotationally positioning the screw rods 5.

[0052] In this embodiment, a plurality of guide rods are fixedly installed on the top of the piston 6, and a circular ring 27 is slidably sleeved on the outside of the guide rod to guide the circular ring 27. A U-shaped rod 17 is fixedly installed on the left side of the top of the piston 6, and a fixed plate 16 is slidably sleeved on the outside of the U-shaped rod 17 to guide the piston 6.

[0053] In this embodiment, the upper cover 2 is fixed by the cooperation of the buckle plate 4 and the clamping plate 32, and the high-pressure and extreme temperature environment conditions for the microorganisms to live in the marine environment are constructed by the cooperation of the upper cover 2 and the culture chamber 1. By pressing the feed hopper 13 downward, the microorganism enriched bacterial liquid is added from the feed hopper 13 to the liquid storage cylinder 3, and then the feed hopper 13 is released, so that the feed hopper 13 is reset under the elastic force of the support spring 28 and drives the sealing plate 26 to move upward, and the piston 6 is sealed by the sealing plate 26;

[0054] By starting the driving motor 8, one of the screw rods 5 is driven to rotate, and the other two screw rods 5 are driven to rotate synchronously through the cooperation of the three synchronous pulleys 33 and the belt. The three screw rods 5 drive the liquid storage cylinder 3 downward through the threaded transmission with the corresponding connecting plate 31 and under the guidance of the guide column 25. During the upward movement of the liquid storage cylinder 3, the fixed plate 16 and the side plate 18 are driven to move upward synchronously, and the cross plate 10 is driven to rotate clockwise through the second connecting rod 12. The cross plate 10 drives the piston 6 to move downward through the cooperation with the first connecting rod 7, so that the microorganism enriched bacterial liquid in the liquid storage cylinder 3 is pressed into the curved pipe 30 and discharged from the bottom end of the curved pipe 30, so that the microorganism enriched bacterial liquid is dispersed into the culture chamber 1;

[0055] When the liquid storage cylinder 3 moves upward, the vertical plate 38 drives the horizontal shaft 37 and the transmission gear 36 to move upward. The rack 29 drives the horizontal shaft 37 to rotate by meshing with the transmission gear 36, and drives the curved pipe 30 to rotate by meshing with the driving bevel gear 35 and the driven bevel gear 34, so that the curved pipe 30 rotates while rising, so that the movement of the bottom end of the curved pipe 30 is spiral upward, ensuring the dilution uniformity of the enriched bacterial liquid;

[0056] The convex plate 15 is driven to move downward by pulling the pull rod 19 downward, so that the convex plate 15 is disengaged from the positioning groove, and the fixation of the knob 21 is released. Then, the knob 21 is rotated to drive the screw rod 22 to rotate. The screw rod 22 drives the horizontal plate 10 to move horizontally through the threaded transmission with the moving plate 23, so that the ratio of the horizontal plate 10 located at both ends of the installation shaft 11 is changed, thereby adjusting the size of the force arm at both ends of the lever formed by the horizontal plate 10 and the installation shaft 11, so as to adjust the speed of the piston 6 moving downward, so as to meet the needs of different output concentrations of the enriched bacterial liquid;

[0057] Pull the annular plate 41 downward to drive the clamping plate 32 out of the clamping slot, and then rotate the upper cover 2 so that the arc-shaped protrusion is out of contact with the buckle plate 4, thereby releasing the fixation of the upper cover 2 and facilitating the removal of the upper cover 2.

[0058] The above is a detailed introduction to a high-pressure environment marine microorganism enrichment culture and gravity separation device provided by the present invention. Specific embodiments are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. It should be pointed out that for ordinary technicians in this technical field, without departing from the principle of the present invention, the present invention can also be improved and modified, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A high-pressure environment marine microorganism enrichment culture and gravity separation device, characterized in that: include: A culture chamber (1), wherein the top of the culture chamber (1) is open; An upper cover (2), the upper cover (2) being detachably mounted on the top of the culture chamber (1); A liquid storage cylinder (3), wherein the bottom of the liquid storage cylinder (3) is rotatably connected to a curved tube (30), the bottom end of the curved tube (30) penetrates the upper cover (2) and extends into the culture chamber (1), and a piston (6) is slidably and sealably installed in the liquid storage cylinder (3), the top of the piston (6) is hinged to two first connecting rods (7), the two first connecting rods (7) are hinged to a transverse plate (10) on the sides close to each other, the front and rear sides of the transverse plate (10) are rotatably connected to second connecting rods (12), and the two second connecting rods ( 12) The bottom ends of the sides close to each other are rotatably connected to the same side plate (18), the right side of the side plate (18) is fixedly connected to a fixed plate (16), the fixed plate (16) is fixedly connected to the left side of the liquid storage cylinder (3), the outer side of the horizontal plate (10) is slidably sleeved with a sliding frame (9), the front and rear sides of the sliding frame (9) are fixedly connected to a mounting shaft (11), the outer side of the mounting shaft (11) is rotatably sleeved with a vertical plate (14), and the vertical plate (14) is fixedly connected to the top of the upper cover (2); A drive assembly, the drive assembly comprising three connecting plates (31), three screw rods (5) and three synchronous pulleys (33), the three synchronous pulleys (33) being provided with a same belt for transmission, and the three synchronous pulleys (33) being respectively fixedly sleeved on the outer sides of the corresponding screw rods (5), the bottom ends of the screw rods (5) being rotatably connected to the top of the upper cover (2), the connecting plates (31) being fixedly connected to the outer sides of the liquid storage cylinder (3), and the connecting plates (31) being threadedly sleeved on the outer sides of the corresponding screw rods (5); A linkage assembly, the linkage assembly comprising a driven bevel gear (34), a driving bevel gear (35), a transmission gear (36) and a rack (29), the rack (29) being fixedly connected to the top of the upper cover (2), a transverse shaft (37) being connected between the driving bevel gear (35) and the transmission gear (36), the transverse shaft (37) being rotatably mounted on the bottom of the liquid storage cylinder (3), and the driven bevel gear (34) being meshed with the driving bevel gear (35); Two vertical plates (38) are fixedly installed on the top of the horizontal plate (10), and a screw rod (22) is rotatably connected between the two vertical plates (38). A movable plate (23) is fixedly installed on the top of the sliding frame (9), and the movable plate (23) is threadedly sleeved on the outside of the screw rod (22), and a knob (21) is fixedly connected to the left end of the screw rod (22); a circular hole is opened on the top of the piston (6), and a feed hopper (13) is slidably installed in the circular hole, and a sealing plate (26) is fixedly installed on the bottom of the feed hopper (13), and the sealing plate (26) is movably abutted against the bottom of the piston (6), and a circular ring (27) is fixedly installed on the outside of the feed hopper (13), and a support spring (28) is fixedly connected to the bottom of the circular ring (27), and the bottom ends of multiple support springs (28) are fixedly connected to the top of the piston (6); Two mounting plates and a plurality of vertical rods are fixedly mounted on the bottom of the liquid storage cylinder (3), and the two mounting plates are rotatably sleeved on the outside of the horizontal axis (37). The bottom ends of the vertical rods are fixedly connected to a limiting frame (39), and the outside of the curved tube (30) is fixedly sleeved with a limiting ring (40), and the limiting frame (39) movably abuts against the outside of the limiting ring (40); a square groove is provided on the top of the horizontal plate (10), and a convex plate (15) is slidably mounted in the square groove. A plurality of positioning grooves are provided on the outside of the knob (21), and the convex plate (15) is movably clamped in the corresponding positioning grooves. A pull rod (19) and a compression spring (20) are fixedly connected to the bottom plate of the convex plate (15), and the bottom end of the compression spring (20) is fixedly connected to the bottom inner wall of the corresponding square groove; A buckle plate (4) is fixedly installed on both sides of the top of the culture chamber (1), the buckle plate (4) is movably abutted against the outer side of the upper cover (2), and both sides of the upper cover (2) are integrally formed with arc-shaped protrusions, and the two buckle plates (4) are movably abutted against the tops of the corresponding arc-shaped protrusions, and vertical holes are provided on both sides of the top of the culture chamber (1), and a clamping plate (32) is slidably installed in the vertical holes, and a clamping groove is provided on both sides of the bottom of the upper cover (2), and the two clamping plates (32) are movably clamped in the corresponding clamping grooves, and the bottoms of the two clamping plates (32) are fixedly connected to the same annular plate (41), the annular plate (41) is slidably sleeved on the outer side of the culture chamber (1), and the bottom of the annular plate (41) is fixedly connected to a plurality of connecting springs (42), and a fixing ring is fixedly sleeved on the outer side of the culture chamber (1), and the bottom ends of the connecting springs (42) are fixedly connected to the top of the fixing ring.

2. A high pressure environment marine microorganism enrichment culture and gravity separation device according to claim 1, characterized in that: A motor seat is fixedly mounted on the front side of the rear upright plate (14) of the two upright plates (14), a driving motor (8) is fixedly mounted on the bottom of the motor seat, and an output shaft of the driving motor (8) is fixedly connected to the top end of the corresponding screw rod (5).

3. The high-pressure environment marine microorganism enrichment culture and gravity separation device according to claim 1, characterized in that: Three guide columns (25) are fixedly connected to the top of the upper cover (2), and the three connecting plates (31) are respectively slidably sleeved on the outer sides of the corresponding guide columns (25). A positioning plate (24) is fixedly installed on the top of the guide column (25), and the three positioning plates (24) are respectively rotatably sleeved on the outer sides of the corresponding screw rods (5).

4. The high-pressure environment marine microorganism enrichment culture and gravity separation device according to claim 1, characterized in that: A plurality of guide rods are fixedly mounted on the top of the piston (6), and the circular ring (27) is slidably sleeved on the outside of the guide rods.

5. The high-pressure environment marine microorganism enrichment culture and gravity separation device according to claim 1, characterized in that: A U-shaped rod (17) is fixedly mounted on the left side of the top of the piston (6), and the fixing plate (16) is slidably sleeved on the outside of the U-shaped rod (17).

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