Method for alleviating saline-alkali stress and promoting seed germination of kudzu vine

Abstract

A method for alleviating saline-alkali stress and promoting seed germination of kudzu vine, comprising the following steps: S1, preparing kudzu vine seeds with full grains and uniform size; S2, placing the kudzu vine seeds in a culture dish with a thickness of 1-2 mm; S3, placing the culture dish containing the kudzu vine seeds in a high-voltage electrostatic field treatment device, and treating with high-voltage electrostatic field for 55-75 min; the voltage of the high-voltage electrostatic field treatment is 14-16 kv; S4, taking out the seeds and planting them in saline-alkali soil; the high-voltage electrostatic field treatment device comprises a controller, a transformer and a seed treatment device; the controller and the seed treatment device are electrically connected through wires and the transformer; the seed treatment device comprises an insulating shell and an insulating bottom plate, the insulating bottom plate is movably arranged at the bottom end of the insulating shell, and the bottom end of the insulating shell is closed; the present application provides a method for alleviating saline-alkali stress and promoting seed germination of kudzu vine, which has the advantages of simple operation, low energy consumption, green and pollution-free, fast drying speed and sterilization.

Description

A method to alleviate salt and alkali stress and promote the germination of Platycodon grandiflorus seeds Technical Field

[0001] This invention relates to the field of plant cultivation technology, and more specifically, to a method for alleviating salt and alkali stress and promoting the germination of Platycodon grandiflorus seeds. Background Technology

[0002] Soil salinization is a serious problem in the ecological environment. The accumulation of soluble salts in the soil changes the properties of the soil, which in turn makes it difficult for crop seeds to germinate and sprout, seriously restricting the development of agriculture and medicinal plants.

[0003] Platycodon grandiflorum (Jacq.) A.DC, a traditional Chinese medicine, is the dried root of Platycodon grandiflorum, a plant in the Campanulaceae family. It has the effects of clearing the lungs, relieving sore throat, resolving phlegm, and draining pus. It is both a food and a medicine, but wild resources are scarce, and it is currently mainly obtained through artificial cultivation and propagation by seed. However, during cultivation, soil salinization makes it difficult for Platycodon grandiflorum seeds to germinate, resulting in low seedling emergence rates, which seriously affects its yield and quality.

[0004] Currently, pre-sowing treatment techniques for bellflower seeds mostly employ chemical agents. However, this method sometimes fails to effectively mitigate the impact of saline-alkali soil on seed germination, and long-term use can lead to pesticide resistance in pests and diseases. Furthermore, the use of chemical agents pollutes the environment; chemically treated seeds, if not planted, can only be disposed of as waste and cannot be reused as animal feed. Therefore, finding methods to improve the efficient germination of bellflower seeds in saline-alkali environments is of significant practical importance and helps solve the root causes of problems in large-scale bellflower production. Summary of the Invention

[0005] In view of the above-mentioned technical problems, the present invention provides a method for alleviating salt-alkali stress and promoting the germination of Platycodon grandiflorus seeds. This method is simple to operate, green, and pollution-free, and can effectively improve the germination rate of Platycodon grandiflorus seeds in saline-alkali soil, increase the seedling emergence rate, and solve the source problem in large-scale production of Platycodon grandiflorus. The method includes the following steps:

[0006] S1. Prepare plump and uniformly sized bellflower seeds;

[0007] S2. Place the bellflower seeds in a petri dish, 1-2 mm thick;

[0008] S3. Place the culture dish containing the Platycodon grandiflorus seeds in a high-voltage electrostatic field treatment device and perform high-voltage electrostatic treatment for 55-75 minutes; the voltage of the high-voltage electrostatic field treatment is 14-16 kV.

[0009] The high-voltage electrostatic field treatment device includes a controller, a transformer, and a seed treatment device; the controller is electrically connected to the transformer and the seed treatment device via wires.

[0010] The seed treatment device includes an insulating outer shell and an insulating base plate, wherein the insulating base plate is movably disposed at the bottom end of the insulating outer shell to close the bottom end of the insulating outer shell;

[0011] An insulating post is fixedly connected to each of the four corners of the insulating base plate. A conductive metal frame is movably mounted on each insulating post. The conductive metal frame consists of two vertical conductive metal rods and several horizontal conductive metal rods. The horizontal conductive metal rods are arranged side by side on the vertical conductive metal rods. A discharge nail is movably connected to each horizontal conductive metal rod.

[0012] The distance from the insulating base plate to the tip of the discharge nail is 4-6cm;

[0013] S4. Remove the seeds and plant them in saline-alkali soil.

[0014] Furthermore, a method for alleviating salt-alkali stress and promoting the germination of Platycodon grandiflorus seeds includes the following steps:

[0015] S1. Prepare plump and uniformly sized bellflower seeds;

[0016] S2. Place the bellflower seeds in a petri dish, 1.5 mm thick;

[0017] S3. Place the petri dish containing the bellflower seeds into a high-voltage electrostatic field treatment device and perform high-voltage electrostatic treatment for 65 minutes; the voltage of the high-voltage electrostatic field treatment is 15kV.

[0018] S4. Remove the seeds and plant them in saline-alkali soil;

[0019] The distance from the insulating base plate to the tip of the discharge nail is 6cm.

[0020] Furthermore, one end of the wire is electrically connected to the transformer, and the other end is connected to a metal tweezer, which is clamped at any point on the conductive metal frame.

[0021] Furthermore, the insulating post includes an upper post and a lower post, with the upper post inserted into the lower post to adjust the height of the insulating post.

[0022] Furthermore, a groove is provided on the lower side of the transverse conductive metal rod, and a fixing buckle is integrally connected to the upper part of the discharge nail. A groove is provided between the fixing buckle and the discharge nail. The inner diameter of the groove of the discharge nail is less than or equal to the width of the groove. The groove of the discharge nail is engaged in the groove and can slide freely in the groove.

[0023] Furthermore, both the vertical and horizontal conductive metal rods are provided with scales, and the horizontal conductive metal rod can slide freely on the vertical conductive metal rod to adjust its width.

[0024] Furthermore, several petri dishes are movably placed on the insulating base plate.

[0025] Furthermore, the longitudinal distance between two adjacent discharge nails is 2-4 cm, and the lateral distance is 3-5 cm.

[0026] Furthermore, the longitudinal distance between two adjacent discharge nails is 3cm and the lateral distance is 4cm.

[0027] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0028] 1. This invention provides a method for relieving salt and alkali stress and promoting the germination of Platycodon grandiflorus seeds, which has the advantages of simple operation, low energy consumption, green and pollution-free operation, fast drying speed and sterilization.

[0029] 2. The method for alleviating salt-alkali stress and promoting the germination of Platycodon grandiflorus seeds provided by the present invention, when the distance from the insulating base plate to the tip of the discharge nail is 5-7 cm, the high-voltage electrostatic treatment is 55-75 min, and the voltage of the high-voltage electrostatic field treatment is 14-16 kV, the germination rate of Platycodon grandiflorus seeds under 150 mmol / L salt-alkali stress is relatively high, at 16.66%, 18.66%, and 15.33%, respectively; preferably, when the distance from the insulating base plate to the tip of the discharge nail is 6 cm, the voltage is 15 kV, and the treatment time is 65 min, the germination rate of Platycodon grandiflorus seeds under 150 mmol / L salt-alkali stress is the highest, at 18.66%.

[0030] 3. Existing high-voltage electrostatic field treatment devices, as shown in Figure 9, use an anvil for discharge. The discharge current is too high and cannot be adjusted, and the distance from the seeds is also difficult to adjust. This invention provides a new high-voltage electrostatic field treatment device, including a controller, a transformer, and a seed treatment device. The controller is electrically connected to the transformer and the seed treatment device via wires. The seed treatment device includes an insulating shell and an insulating base plate. The insulating base plate is movably disposed at the bottom of the insulating shell, sealing the bottom of the shell. An insulating column is fixedly connected to each of the four corners of the insulating base plate, and a conductive metal frame is movably mounted on each insulating column. The conductive metal frame consists of two vertical conductive metal rods and several horizontal conductive metal rods. The horizontal conductive metal rods are arranged side-by-side on the vertical conductive metal rods, and discharge nails are movably connected to the horizontal conductive metal rods. The distance from the insulating base plate to the tip of the discharge nail is 5-7 cm. The longitudinal distance between two adjacent discharge nails is 2-4 cm, and the lateral distance is 3-5 cm. The insulating columns are adjustable in height, making them convenient to use and allowing for control of the discharge current. Attached Figure Description

[0031] Figure 1 shows the effect of mixed salt and alkali stress on the germination rate of Platycodon grandiflorus seeds;

[0032] Figure 2 shows the effect of different electric field strengths and treatment times on the germination rate of Platycodon grandiflorus under salt-alkali stress when the distance from the insulating base plate to the tip of the discharge nail is 4 cm (Experiment 2).

[0033] Figure 3 shows the effect of different electric field strengths and treatment times on the germination rate of Platycodon grandiflorus under salt-alkali stress when the distance from the insulating base plate to the tip of the discharge nail is 6 cm (Experiment 3).

[0034] Figure 4 shows the effect of different electric field strengths and treatment times on the germination rate of Platycodon grandiflorus under salt-alkali stress when the distance from the insulating base plate to the tip of the discharge nail is 8 cm (Experiment 4).

[0035] Figure 5 is an overall schematic diagram of the high-voltage electrostatic field treatment device of the present invention;

[0036] Figure 6 is an enlarged view of part A in Figure 5;

[0037] Figure 7 is a schematic diagram of the connection between the discharge nail and the conductive metal frame of the present invention;

[0038] Figure 8 is a cross-sectional view of the seed treatment device;

[0039] Figure 9 shows a high-voltage electrostatic field treatment device in the prior art.

[0040] In the diagram: 1. Controller; 2. Seed treatment device; 3. Wire; 4. Metal tweezers; 5. Insulating shell; 6. Insulating base plate; 7. Insulating column; 71. Upper column; 72. Lower column; 8. Conductive metal frame; 81. Vertical conductive metal rod; 82. Horizontal conductive metal rod; 83. Slide; 84. Fixing buckle; 85. Groove; 9. Petri dish; 10. Discharge nail; 11. Transformer. Detailed Implementation Example 1

[0041] A method for alleviating salt-alkali stress and promoting the germination of Platycodon grandiflorus seeds includes the following steps:

[0042] S1. Prepare plump and uniformly sized bellflower seeds;

[0043] S2. Place the bellflower seeds in a petri dish, 1 mm thick;

[0044] S3. Place the petri dish containing the bellflower seeds into a high-voltage electrostatic field treatment device and perform high-voltage electrostatic treatment for 55 minutes; the voltage of the high-voltage electrostatic field treatment is 14 kV.

[0045] The high-voltage electrostatic field treatment device includes a controller 1, a transformer 11, and a seed treatment device 2; the controller 1 is electrically connected to the transformer 11 and the seed treatment device 2 via a wire 3.

[0046] The seed treatment device 2 includes an insulating outer shell 5 and an insulating base plate 6. The insulating base plate 6 is movably disposed at the bottom end of the insulating outer shell 5, thereby closing the bottom end of the insulating outer shell 5.

[0047] An insulating post 7 is fixedly connected to each of the four corners of the insulating base plate 6. A conductive metal frame 8 is movably mounted on the insulating post 7. The conductive metal frame 8 consists of two vertical conductive metal rods 81 and several horizontal conductive metal rods 82. The horizontal conductive metal rods 82 are arranged side by side on the vertical conductive metal rods 81. A discharge nail 10 is movably connected to the horizontal conductive metal rods 82.

[0048] The distance from the insulating base plate 6 to the tip of the discharge nail 10 is 4cm;

[0049] S4. Remove the seeds and plant them in saline-alkali soil.

[0050] Moreover, one end of the wire 3 is electrically connected to the transformer 11, and the other end is connected to a metal tweezer 4, which is clamped at any point on the conductive metal frame 8.

[0051] Furthermore, the insulating post 7 includes an upper post 71 and a lower post 72, with the upper post 71 inserted into the lower post 72 to adjust the height of the insulating post 7.

[0052] Furthermore, a groove 83 is provided on the lower side of the transverse conductive metal rod 82, and a fixing buckle 84 is integrally connected to the upper part of the discharge nail 10. A groove 85 is provided between the fixing buckle 84 and the discharge nail 10. The inner diameter of the groove 85 of the discharge nail 10 is less than or equal to the width of the groove 83. The groove 85 of the discharge nail 10 is engaged in the groove 83 and can slide freely in the groove 83.

[0053] Furthermore, both the vertical conductive metal rod 81 and the horizontal conductive metal rod 82 are provided with scales, and the horizontal conductive metal rod 82 can slide freely on the vertical conductive metal rod 81 to adjust its width.

[0054] Furthermore, several petri dishes 9 are movably placed on the insulating base plate 6.

[0055] Furthermore, the longitudinal distance between two adjacent discharge nails 10 is 2cm, and the lateral distance is 3cm. Example 2

[0056] A method for alleviating salt-alkali stress and promoting the germination of Platycodon grandiflorus seeds includes the following steps:

[0057] S1. Prepare plump and uniformly sized bellflower seeds;

[0058] S2. Place the bellflower seeds in a petri dish, 2 mm thick;

[0059] S3. Place the culture dish containing the bellflower seeds in a high-voltage electrostatic field treatment device and perform high-voltage electrostatic treatment for 75 minutes; the voltage of the high-voltage electrostatic field treatment is 16kV; the high-voltage electrostatic field treatment device is the same as in Example 1, except that the distance from the insulating base plate 6 to the tip of the discharge nail 10 is adjusted to 8cm.

[0060] The longitudinal distance between two adjacent discharge nails 10 is adjusted to 4cm and the lateral distance is adjusted to 5cm;

[0061] S4. Remove the seeds and plant them in saline-alkali soil. Example 3

[0062] A method for alleviating salt-alkali stress and promoting the germination of Platycodon grandiflorus seeds includes the following steps:

[0063] S1. Prepare plump and uniformly sized bellflower seeds;

[0064] S2. Place the bellflower seeds in a petri dish, 1.5 mm thick;

[0065] S3. Place the culture dish containing the Platycodon grandiflorus seeds in a high-voltage electrostatic field treatment device and treat it for 65 minutes. The voltage of the high-voltage electrostatic field treatment is 15kV. The high-voltage electrostatic field treatment device is the same as in Example 2. Adjust the distance from the insulating base plate 6 to the tip of the discharge nail 10 to 6cm.

[0066] The longitudinal distance between two adjacent discharge nails 10 is adjusted to 3cm and the lateral distance is adjusted to 4cm;

[0067] S4. Remove the seeds and plant them in saline-alkali soil.

[0068] Experimental Section

[0069] In the experiments conducted in this invention, different lowercase letters indicate significant differences between different treatments (p < 0.05).

[0070] Experiment 1

[0071] Two neutral salts, NaCl and Na₂SO₄, and two alkaline salts, Na₂CO₃ and NaHCO₃, were mixed in different proportions to form five treatment groups: A, B, C, D, and E. Each group contained four salt concentrations: 50, 100, 150, and 200 mmol / L. A total of 20 mixed salt-alkali treatment groups with different concentrations and pH values ​​were simulated, as detailed in Table 1. Among the different concentrations, the mixed salt-alkali stress treatment group C (1:9:9:1) represented the concentration typically used for planting this medicinal herb in saline-alkali soil, and it showed the most significant inhibitory effect on the germination of Platycodon grandiflorus seeds (see Figure 1). Therefore, the mixed salt-alkali stress concentration of 150 mmol / L, C (1:9:9:1), was selected as the experimental research object.

[0072] Table 1. Salt composition, molar ratio, and pH value of each treatment group

[0073]

[0074] Using the high-voltage electrostatic field treatment device of the present invention, the distance from the insulating base plate to the tip of the discharge nail was adjusted to 4 cm, and the effects of different electric field strengths and treatment times on the germination rate of Platycodon grandiflorus under salt-alkali stress were tested.

[0075] Experiment 2

[0076] ① The electric field strength is 5kV

[0077] Seven treatment groups were set up: control (distilled water), pure salt-alkali stress 150 mmol / L (C 1:9:9:1), 5 kv + 35 min + 150 mmol / L, 5 kv + 45 min + 150 mmol / L, 5 kv + 55 min + 150 mmol / L, 5 kv + 65 min + 150 mmol / L, and 5 kv + 75 min + 150 mmol / L. The experimental results are shown in Figure 2.

[0078] ② The electric field strength is 10kV

[0079] Seven treatment groups were set up: control (distilled water), pure salt-alkali stress (C 1:9:9:1), 10kv+35min+150mmol / L, 10kv+45min+150mmol / L, 10kv+55min+150mmol / L, 10kv+65min+150mmol / L, and 10kv+75min+150mmol / L. The experimental results are shown in Figure 2.

[0080] ③ The field strength is 15kV

[0081] Seven treatments were set up: control (distilled water), pure salt-alkali stress (C 1:9:9:1), 15kv 35min + 150mmol / L, 15kv 45min + 150mmol / L, 15kv 55min + 150mmol / L, 15kv 65min + 150mmol / L, and 15kv 75min + 150mmol / L. The experimental results are shown in Figure 2.

[0082] ④ The field strength is 20kV

[0083] Seven treatments were set up: control (distilled water), pure salt-alkali stress (C 1:9:9:1), 20kv 35min + 150mmol / L, 20kv 45min + 150mmol / L, 20kv 55min + 150mmol / L, 20kv 65min + 150mmol / L, and 20kv 75min + 150mmol / L. The experimental results are shown in Figure 2.

[0084] The optimal experimental results of this experiment are listed in Table 2.

[0085] As shown in Figure 2, when the distance from the insulating base plate to the tip of the discharge nail is 4 cm, the treatment of 10 kV 55 min + 150 mmol / L improves the germination rate of Platycodon grandiflorus seeds under salt-alkali stress. Compared with the pure salt-alkali stress group, the germination rate of Platycodon grandiflorus seeds increased by 10.66%.

[0086] Experiment 3

[0087] In Experiment 1, the distance from the insulating base plate to the tip of the discharge nail was adjusted to 6 cm to test the effect of different electric field strengths and treatment times on the germination rate of Platycodon grandiflorus under salt-alkali stress.

[0088] ① The electric field strength is 5kV

[0089] Seven treatment groups were set up: control (distilled water), pure salt-alkali stress 150 mmol / L (C 1:9:9:1), 5 kv + 35 min + 150 mmol / L, 5 kv + 45 min + 150 mmol / L, 5 kv + 55 min + 150 mmol / L, 5 kv + 65 min + 150 mmol / L, and 5 kv + 75 min + 150 mmol / L. The experimental results are shown in Figure 3.

[0090] ② The electric field strength is 10kV

[0091] Seven treatment groups were set up: control (distilled water), pure salt-alkali stress (C 1:9:9:1), 10kv+35min+150mmol / L, 10kv+45min+150mmol / L, 10kv+55min+150mmol / L, 10kv+65min+150mmol / L, and 10kv+75min+150mmol / L. The experimental results are shown in Figure 3.

[0092] ③ The field strength is 15kV

[0093] Seven treatments were set up: control (distilled water), pure salt-alkali stress 150 mmol / L (C 1:9:9:1), 15 kv 35 min + 150 mmol / L, 15 kv 45 min + 150 mmol / L, 15 kv 55 min + 150 mmol / L, 15 kv 65 min + 150 mmol / L, and 15 kv 75 min + 150 mmol / L. The experimental results are shown in Figure 3.

[0094] ④ The field strength is 20kV

[0095] Seven treatments were set up: control (distilled water), pure salt-alkali stress 150 mmol / L (C 1:9:9:1), 20 kv 35 min + 150 mmol / L, 20 kv 45 min + 150 mmol / L, 20 kv 55 min + 150 mmol / L, 20 kv 65 min + 150 mmol / L, and 20 kv 75 min + 150 mmol / L. The experimental results are shown in Figure 3.

[0096] The optimal experimental results of this experiment are listed in Table 2.

[0097] As shown in Figure 3, the treatments of 15kv+55min+150mmol / L, 15kv+65min+150mmol / L, and 15kv+75min+150mmol / L significantly improved the germination rate of Platycodon grandiflorus seeds under salt-alkali stress, with increases of 16.66%, 18.66%, and 15.33% respectively compared to the pure salt-alkali stress group.

[0098] Experiment 4

[0099] In Experiment 1, the distance from the insulating base plate to the tip of the discharge nail was adjusted to 8 cm to test the effect of different electric field strengths and treatment times on the germination rate of Platycodon grandiflorus under salt-alkali stress.

[0100] ① The electric field strength is 5kV

[0101] Seven treatment groups were set up: control (distilled water), pure salt-alkali stress 150 mmol / L (C 1:9:9:1), 5 kv + 35 min + 150 mmol / L, 5 kv + 45 min + 150 mmol / L, 5 kv + 55 min + 150 mmol / L, 5 kv + 65 min + 150 mmol / L, and 5 kv + 75 min + 150 mmol / L. The experimental results are shown in Figure 4.

[0102] ② The electric field strength is 10kV

[0103] Seven treatment groups were set up: control (distilled water), pure salt-alkali stress (C 1:9:9:1), 10kv+35min+150mmol / L, 10kv+45min+150mmol / L, 10kv+55min+150mmol / L, 10kv+65min+150mmol / L, and 10kv+75min+150mmol / L. The experimental results are shown in Figure 4.

[0104] ③ The field strength is 15kV

[0105] Seven treatments were set up: control (distilled water), pure salt-alkali stress (C 1:9:9:1), 15kv 35min + 150mmol / L, 15kv 45min + 150mmol / L, 15kv 55min + 150mmol / L, 15kv 65min + 150mmol / L, and 15kv 75min + 150mmol / L. The experimental results are shown in Figure 4.

[0106] ④ The field strength is 20kV

[0107] Seven treatments were set up: control (distilled water), pure salt-alkali stress (C 1:9:9:1), 20kv 35min+150mmol / L, 20kv 45min+150mmol / L, 20kv 55min+150mmol / L, 20kv 65min+150mmol / L, and 20kv 75min+150mmol / L. The experimental results are shown in Figure 4.

[0108] The optimal experimental results of this experiment are listed in Table 2.

[0109] As shown in Figure 4, the treatment of 15 kv + 45 min + 150 mmol / L significantly improved the germination rate of Platycodon grandiflorus seeds under salt-alkali stress, with an increase of 8.66% compared to the pure salt-alkali stress group.

[0110] Table 2 Optimal experimental results of Experiments 2-4

[0111]

[0112] Based on Experiment 4, Figure 4, and Table 2, it can be concluded that in saline-alkali soil conditions, the optimal solution in Experiment 4 is when the distance from the insulating base plate to the tip of the discharge nail is 8 cm, the voltage is 15 kV, and the treatment time is 45 min. This solution increases the germination rate of Platycodon grandiflorus seeds under 150 mmol / L saline-alkali stress by 8.66%, which is a relatively poor effect.

[0113] Based on Experiment 2, Figure 2 and Table 2 above, it can be seen that the optimal scheme for Experiment 4 is when the distance from the insulating base plate to the tip of the discharge nail is 4 cm, the voltage is 10 kV, and the treatment time is 55 min. This scheme increases the germination rate of Platycodon grandiflorus seeds under 150 mmol / L salt and alkali stress by 10.66%, but the effect is only average.

[0114] Based on Experiment 3, Figure 3, and Table 2, it can be seen that when the distance from the insulating base plate to the tip of the discharge nail is 6 cm, the voltage is 15 kV, and the treatment time is 55 min, 65 min, and 75 min, the germination rate of Platycodon grandiflorus seeds under 150 mmol / L salt-alkali stress is increased by 16.66%, 18.66%, and 15.33%, respectively, showing a significant effect. Preferably, when the distance from the insulating base plate to the tip of the discharge nail is 6 cm, the voltage is 15 kV, and the treatment time is 65 min, the germination rate of Platycodon grandiflorus seeds under 150 mmol / L salt-alkali stress is increased the most, at 18.66%.

[0115] In conclusion, it can be proven that the technical solution of the present invention significantly improves the germination rate of Platycodon grandiflorus seeds under 150 mmol / L salt-alkali stress.

[0116] Note: Throughout the experiment, we observed slight fluctuations in the germination rate of Platycodon grandiflorus seeds without affecting the overall trend. This is because the effect of the high-voltage electrostatic field on seed germination is a microscopic and complex combination of physical, chemical, and biological factors. When the electric field strength and treatment time are appropriate, seeds exposed to the electric field experience increased activity of various metabolic enzymes, antioxidant enzymes such as SOD, POD, and CAT due to the disruption of their cell membrane structure, resulting in energy and mass deposition and enhancing their germination characteristics. Meanwhile, the lower the voltage, the less energy the electric field outputs, and the weaker the intensity of its effect on the seeds, resulting in a less significant impact on seed germination. Furthermore, due to the distance between the discharge pins and between the pins and the seeds within the electric field, seeds directly below the discharge pins receive greater radiation intensity than those further away. Therefore, as the electric field strength and treatment time gradually increase, the uneven distribution of electric field and electromagnetic radiation intensity causes the overall germination characteristics to show an upward trend in the experimental results, while also exhibiting some fluctuations. When the electric field strength increases again, the energy output per unit time becomes greater, and the destructive damage to the seeds far exceeds their repair capabilities. As the duration of the electric field's action increases, the penetrating power of the electromagnetic field surges, making seeds exposed to the electric field highly susceptible to breakdown, leading to a rapid decline in their germination ability.

Claims

1. A method for alleviating salt-alkali stress and promoting the germination of Platycodon grandiflorus seeds, characterized in that, The process includes the following steps: S1, preparing plump and uniformly sized bellflower seeds; S2, placing the bellflower seeds in a petri dish with a thickness of 1-2 mm; S3, placing the petri dish containing the bellflower seeds in a high-voltage electrostatic field treatment device and treating it with high voltage electrostatics for 65 min; the voltage of the high-voltage electrostatic field treatment is 15 kV; the high-voltage electrostatic field treatment device includes a controller (1), a transformer (11), and a seed treatment device (2); the controller (1) is electrically connected to the transformer (11) and the seed treatment device (2) via a wire (3); the seed treatment device (2) includes an insulating shell (5) and an insulating base plate (6), the insulating base plate (6) is movably set at the bottom end of the insulating shell (5) to close the bottom end of the insulating shell (5); an insulating column (7) is fixedly connected to each of the four corners of the insulating base plate (6), and a conductive metal frame (8) is movably set on the insulating column (7); the conductive metal frame (8) It consists of two vertical conductive metal rods (81) and several horizontal conductive metal rods (82). The horizontal conductive metal rods (82) are arranged side by side on the vertical conductive metal rods (81). A discharge nail (10) is movably connected to the horizontal conductive metal rod (82). A sliding groove (83) is opened on the lower side of the horizontal conductive metal rod (82). A fixing buckle (84) is integrally connected to the upper part of the discharge nail (10). A groove (85) is opened between the fixing buckle (84) and the discharge nail (10). The inner diameter of the groove (85) of the discharge nail (10) is less than or equal to the width of the sliding groove (83). The groove (85) of the discharge nail (10) is engaged in the sliding groove (83) and can slide freely in the sliding groove (83). The distance from the insulating base plate (6) to the tip of the discharge nail (10) is 6cm. The longitudinal distance between two adjacent discharge nails (10) is 3cm and the transverse distance is 4cm. S4. Remove the seeds and plant them in saline-alkali soil.

2. The method for alleviating salt-alkali stress and promoting the germination of Platycodon grandiflorus seeds as described in claim 1, characterized in that, One end of the wire (3) is electrically connected to the transformer (11), and the other end is connected to a metal tweezer (4), which is clamped at any point on the conductive metal frame (8).

3. The method for alleviating salt-alkali stress and promoting the germination of Platycodon grandiflorus seeds as described in claim 1, characterized in that, The insulating post (7) includes an upper post (71) and a lower post (72), with the upper post (71) inserted into the lower post (72) to adjust the height of the insulating post (7).

4. The method for alleviating salt-alkali stress and promoting the germination of Platycodon grandiflorus seeds as described in claim 1, characterized in that, Both the vertical conductive metal rod (81) and the horizontal conductive metal rod (82) are provided with scales. The horizontal conductive metal rod (82) can slide freely on the vertical conductive metal rod (81) to adjust the width.

5. The method for alleviating salt-alkali stress and promoting the germination of Platycodon grandiflorus seeds as described in claim 1, characterized in that, Several petri dishes (9) are movably placed on the insulating base plate (6).

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