An endoscope
By coating the endoscope surface with an aminated multi-walled carbon nanotube nanocoating, the toxicity and damage caused by the release of silver ions from the coating were resolved, resulting in improved long-term stability and biocompatibility, and reduced infection risk.
Patent Information
- Application Number
- CN202311340418.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-10-17
- Publication Date
- 2026-01-02
- Estimated Expiration
- 2043-10-17
AI Technical Summary
The silver ion nanocoating on the surface of existing endoscopes gradually releases during use, which weakens the antibacterial effect and may produce toxicity and side effects, while also posing a risk of damage to internal organs and the urethra.
An aminated multi-walled carbon nanotube nanocoating is applied to the endoscope lens and surrounding tube wall surface. The preparation method includes soaking, shaking and drying processes to ensure uniform coating adhesion, providing long-term stability and biocompatibility, and reducing friction damage and infection risk.
Aminated multi-walled carbon nanotube coatings exhibit long-term stability, improve the biocompatibility of endoscopes, reduce friction damage and infection risks, avoid toxicity and side effects, and do not rely on the antibacterial effect released by the material.
Smart Images
Figure CN117531052B_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application belongs to the technical field of medical health, and particularly relates to an endoscope. BACKGROUND
[0002] Intraoperative infection is one of the most common medical accidents in medical institutions, and the incidence of intraoperative infection worldwide is as high as 3-16% every year, which is more common in low-income countries.
[0003] At present, the methods for dealing with intraoperative infection in clinical practice mainly include prevention, early diagnosis and treatment, etc., but these methods also have some shortcomings and deficiencies. Because of the existence of infection sources in the surgical site, long operation time, low immunity of patients, etc., even if sterile instruments and appropriate surgical clothing are used, infection may still occur during the operation.
[0004] Therefore, how to reduce the occurrence of intraoperative infection has attracted more and more widespread attention. SUMMARY
[0005] The present application is made based on the discovery and understanding of the inventors on the following facts and problems:
[0006] In order to reduce infection during surgery and reduce damage to internal organs and urethra, a silver ion nano coating is coated on the surface of the endoscope in the prior art, which can reduce the risk of infection during surgery, but the silver ion will gradually release during use, resulting in weakening of the antibacterial effect, accumulation of silver ion in the body, potential toxicity and side effects, and the silver ion may have irritancy and toxicity to some artificial materials.
[0007] The present application aims to at least solve one of the technical problems in the related art. To this end, an embodiment of the present application proposes an endoscope, the lens and the surrounding tube wall surface of which are covered with a nano coating, which can effectively reduce the occurrence of infection and thrombosis and other complications during surgery, reduce the friction coefficient of the surface of the endoscope, improve the smoothness and wear resistance of the surface, reduce the damage to internal organs and urethra, and has long-term performance stability and biocompatibility, avoiding the occurrence of potential toxicity and side effects.
[0008] An endoscope according to an embodiment of the present application, the lens and the surrounding tube wall surface of which are covered with a nano coating;
[0009] The nano coating is prepared from an aqueous solution of aminated multi-walled carbon nanotubes.
[0010] The endoscope of the embodiment of the present application has the advantages and technical effects that: 1. In the embodiment of the present application, the amino-functionalized multi-walled carbon nanotubes have high hydrophilicity, can adsorb and destroy microbial cell walls, and can effectively reduce the friction damage to organs; 2. In the embodiment of the present application, the amino-functionalized multi-walled carbon nanotubes can provide long-term performance stability, so that the endoscope covered with the nano coating has a longer service life; 3. In the embodiment of the present application, the endoscope modified by the nano coating containing the amino-functionalized multi-walled carbon nanotubes has higher biocompatibility, and can reduce the occurrence of irritation and rejection reactions; 4. In the embodiment of the present application, the endoscope modified by the amino-functionalized multi-walled carbon nanotubes is not dependent on the release of the material in terms of antibacterial effect, and potential toxicity and side effects are avoided.
[0011] In some embodiments, the thickness of the nano coating is 5-10 μm.
[0012] In some embodiments, the preparation method of the nano coating comprises the following steps:
[0013] (1) After the endoscope lens and the surrounding tube wall are treated by immersion in an organic solvent, water is used for rinsing;
[0014] (2) The endoscope lens and the surrounding tube wall treated by the step (1) are immersed in an aqueous solution of amino-functionalized multi-walled carbon nanotubes, and are subjected to shaking treatment in a horizontal shaking bed, and after being taken out, are naturally air-dried.
[0015] In some embodiments, in the step (1), the organic solvent comprises chloroform.
[0016] In some embodiments, in the step (1), the immersion treatment time is 3-4 min.
[0017] In some embodiments, in the step (2), the concentration of the aqueous solution of amino-functionalized multi-walled carbon nanotubes is 0.1-0.5 mg / mL.
[0018] In some embodiments, in the step (2), the shaking treatment time is 5-6 h.
[0019] In some embodiments, in the step (2), the frequency of shaking in the horizontal shaking bed is 80-100 rpm.
[0020] In some embodiments, in the step (2), before the natural air-drying, pure water is used for rinsing. BRIEF DESCRIPTION OF DRAWINGS
[0021] Figure 1 is a photo of the gold staphylococcus after being cultured in the diluted 10 times aqueous solution of amino-functionalized multi-walled carbon nanotubes, wherein the right side is a photo after being enlarged by 35 times.
[0022] Figure 2 is the photo of the Staphylococcus aureus after being cultured with the amino-functionalized multi-walled carbon nanotube aqueous solution diluted by 100 times, wherein the right side is the photo after being enlarged by 35 times;
[0023] Figure 3 is the photo of the Staphylococcus aureus after being cultured with the silver nitrate aqueous solution diluted by 10 times, wherein the right side is the photo after being enlarged by 35 times;
[0024] Figure 4 is the photo of the Staphylococcus aureus after being cultured with the silver nitrate aqueous solution diluted by 100 times, wherein the right side is the photo after being enlarged by 35 times;
[0025] Figure 5 is the photo of the Staphylococcus aureus after being cultured with the silver nitrate aqueous solution diluted by 10 times, wherein the right side is the photo after being enlarged by 35 times;
[0026] Figure 6 is the photo of the Staphylococcus aureus after being cultured with the silver nitrate aqueous solution diluted by 100 times, wherein the right side is the photo after being enlarged by 35 times. DETAILED DESCRIPTION
[0027] Embodiments of the present application are described in detail below with reference to the accompanying drawings. The embodiments described below by reference to the drawings are exemplary and are intended to explain the present application, and cannot be understood as a limitation of the present application.
[0028] An endoscope according to an embodiment of the present application, wherein a lens and a surrounding tube wall surface of the endoscope are covered with a nano coating;
[0029] The nano coating is prepared from an amino-functionalized multi-walled carbon nanotube aqueous solution.
[0030] The endoscope according to an embodiment of the present application, the amino-functionalized multi-walled carbon nanotube has high hydrophilicity, can adsorb and destroy microbial cell walls while effectively reducing frictional damage to organs; the amino-functionalized multi-walled carbon nanotube can provide long-term performance stability, enabling the endoscope covered with the nano coating to have a longer service life; the endoscope modified with the nano coating containing the amino-functionalized multi-walled carbon nanotube has higher biocompatibility, which can reduce the occurrence of irritation and rejection; the endoscope modified with the amino-functionalized multi-walled carbon nanotube is not dependent on the release of the material in terms of antibacterial effect, avoiding potential toxicity and side effects.
[0031] In some embodiments, preferably, the thickness of the nano coating is 5-10 μm.
[0032] In the embodiment of the present application, the thickness of the nano coating is preferred, which is beneficial to further improve the antibacterial effect of the nano coating; if the thickness of the coating is too large, not only the mechanical properties such as bending property and wear resistance will be reduced, but also the production cost will be increased; if the thickness of the coating is too small, it is not conducive to improve the antibacterial effect of the coating, in addition, the adhesion of the coating is weak, which is easy to fall off, affecting the durability and stability of the coating.
[0033] In some embodiments, the preparation method of the nano coating comprises the following steps:
[0034] (1) After the endoscope lens and the surrounding pipe wall are treated by immersion in an organic solvent, they are washed with water;
[0035] (2) The endoscope lens and the surrounding pipe wall treated by the step (1) are immersed in an aqueous solution of amino-functionalized multi-walled carbon nanotubes and are shaken in a horizontal shaker, and after being taken out, they are naturally dried.
[0036] In the embodiment of the present application, the preparation method of the nano coating is preferred, the endoscope lens and the surrounding pipe wall are first treated by immersion, so that the endoscope lens and the surrounding pipe wall are expanded, the gap between the endoscope lens and the surrounding pipe wall is enlarged, which is beneficial to the adhesion of the amino-functionalized multi-walled carbon nanotubes on the surface of the endoscope lens and the surrounding pipe wall, and then the endoscope lens and the surrounding pipe wall are immersed in an aqueous solution of amino-functionalized multi-walled carbon nanotubes and are shaken in a shaker, which can make the amino-functionalized multi-walled carbon nanotubes fully contact with the endoscope lens and the surrounding pipe wall, be fixed on the surface of the endoscope lens and the surrounding pipe wall, and prevent the precipitation of the amino-functionalized multi-walled carbon nanotubes, so that the amino-functionalized multi-walled carbon nanotubes fully contact with the endoscope lens and the surrounding pipe wall and are more uniformly distributed on the surface of the endoscope lens and the surrounding pipe wall.
[0037] In some embodiments, preferably, in the step (1), the organic solvent comprises chloroform. Further preferably, in the step (1), the immersion treatment time is 3-4 min.
[0038] In the embodiment of the present application, the immersion treatment time is preferred, if the immersion time is too long, the endoscope lens and the surrounding pipe wall may be excessively expanded and cannot be retracted; if the immersion time is too short, the endoscope lens and the surrounding pipe wall are not sufficiently expanded, which is not conducive to the adhesion and fixation of the amino-functionalized multi-walled carbon nanotubes on the surface thereof.
[0039] In some embodiments, preferably, in the step (2), the concentration of the aqueous solution of amino-functionalized multi-walled carbon nanotubes is 0.1-0.5 mg / mL.
[0040] In some embodiments, preferably, in the step (2), the shaking treatment time is 5-6 h. Further preferably, in the step (2), the shaking frequency in the horizontal shaker is 80-100 rpm.
[0041] In the embodiment of the present application, the time of the oscillation treatment is preferably selected to enable the retraction of the inflated endoscope lens and the surrounding tube wall.
[0042] In some embodiments, preferably, before the natural air-drying, the endoscope lens and the surrounding tube wall are first washed with pure water.
[0043] In the embodiment of the present application, the washing with pure water before the natural air-drying can wash away the un-fixed amino-functionalized multi-walled carbon nanotubes on the surface of the endoscope lens and the surrounding tube wall, so as to avoid the falling-off during use.
[0044] The technical solutions of the present application will be described in detail below with reference to specific embodiments.
[0045] In the embodiment, the amino-functionalized multi-walled carbon nanotubes are prepared by the following method:
[0046] (a) Acyl chloride treatment of carboxylated multi-walled carbon nanotubes: 1.0 g of MWNT-COOH (carboxylated multi-walled carbon nanotubes) is placed in a three-necked flask, 20 mL of dichlorosulfoxide is added, and the mixture is stirred under reflux at 70°C for 24 h. The obtained mixture is filtered under reduced pressure with a polytetrafluoroethylene membrane, repeatedly washed with tetrahydrofuran (THF), and the obtained black powder-like solid is dried under vacuum at 50°C to obtain MWNT-COCl;
[0047] (b) Amidation: MWNT-COCl is placed in a three-necked flask, 5 g of ammonium carbonate is added, and 50 mL of concentrated ammonia is added dropwise. After stirring for 6 h, the solvent is removed by filtration, repeatedly washed with distilled water, and the obtained black powder-like substance is dried under vacuum at 50°C to obtain MWNT-CONH2.
[0048] (c) Hofmann elimination reaction: MWNT-CONH2 obtained is placed in a three-necked flask, 30 mL of sodium hypochlorite is slowly added dropwise under ice-bath conditions (0-5°C), and after stirring for 30 min, the Hofmann elimination reaction is carried out for 4 h. The obtained product is moved to a 70°C water bath for further reaction for 2 h, filtered under reduced pressure after cooling, and repeatedly washed with distilled water to obtain a black powder-like substance, which is dried under vacuum to obtain MWNT-NH2 (amino-functionalized multi-walled carbon nanotubes).
[0049] Embodiment 1
[0050] (1) The endoscope lens and the surrounding tube wall are soaked in chloroform for 4 min, and after the volume of the endoscope lens and the surrounding tube wall is slightly expanded, they are taken out and washed with a large amount of pure water;
[0051] (2) The endoscope lens and the surrounding tube wall after the treatment of step (1) are immersed in an aqueous solution of the amino-functionalized multi-walled carbon nanotubes with a concentration of 0.1 mg / mL, and are treated by oscillation in a horizontal shaking table, the oscillation frequency is 90 rounds per minute, and the oscillation time is 5 hours. After being taken out, the endoscope lens and the surrounding tube wall are washed with pure water and naturally dried.
[0052] In order to prove that the nano-coating of the amino-functionalized multi-walled carbon nanotubes has a good antibacterial effect, the following test is carried out. The test method is as follows: under the condition of 37℃, Staphylococcus aureus is cultured on a solid medium for 1 hour, then 0.1 mg / mL of the aqueous solution of the amino-functionalized multi-walled carbon nanotubes or 200 mg / L of the aqueous solution of silver nitrate is added dropwise on the surface, and the culture is continued for 48 hours. The number of bacteria on the culture medium is counted, and the culture medium without the addition of the aqueous solution of the amino-functionalized multi-walled carbon nanotubes and the aqueous solution of silver nitrate is used as a blank control group. The results are shown in Table 1, wherein the dilution multiples of 10 -1 and 100 -2 respectively refer to the dilution of the Staphylococcus aureus mother liquor by 10 times and 100 times. The photos of the culture of the amino-functionalized multi-walled carbon nanotubes after the dilution of the Staphylococcus aureus by 10 times and 100 times are shown in Figure 1 and Figure 2 respectively, the photos of the culture of the aqueous solution of silver nitrate after the dilution of the Staphylococcus aureus by 10 times and 100 times are shown in Figure 3 and Figure 4 respectively, and the photos of the culture of the blank control group after the dilution of the Staphylococcus aureus by 10 times and 100 times are shown in Figure 5 and Figure 6 respectively.
[0053] Table 1
[0054]
[0055] It can be seen from the data in Table 1 that after 48 hours of culture, the number of bacteria in the culture medium added with the aqueous solution of the amino-functionalized multi-walled carbon nanotubes is obviously reduced compared with the blank control group, and the antibacterial effect is comparable to that of the aqueous solution of silver nitrate.
[0056] In the present application, the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present application. In the present specification, the illustrative representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, the skilled person in the art can combine and combine the different embodiments or examples described in the present specification and the features of the different embodiments or examples without contradiction.
[0057] Although the above embodiments have been shown and described, it is to be understood that the above embodiments are exemplary, and are not to be construed as limiting the present application, and any changes, modifications, replacements, and variations of the above embodiments made by those skilled in the art are within the scope of the present application.
Claims
1. An endoscope, characterized by, The endoscope lens and the surrounding tube wall surface are covered with a nano coating prepared from an aqueous solution of amino-functionalized multi-walled carbon nanotubes.
2. The endoscope of claim 1, wherein, The thickness of the nano coating is 5-10 microns.
3. The endoscope of claim 1, wherein, The preparation method of the nano coating comprises the following steps: (1) After soaking treatment of the endoscope lens and the surrounding tube wall in an organic solvent, washing with water; (2) Soaking the endoscope lens and the surrounding tube wall treated in step (1) in an aqueous solution of amino-functionalized multi-walled carbon nanotubes and oscillating treatment in a horizontal shaker, and then naturally air-drying after taking out.
4. The endoscope of claim 3, wherein, In step (1), the organic solvent comprises chloroform.
5. The endoscope according to claim 3 or 4, characterized by In step (1), the soaking treatment time is 3-4 minutes.
6. The endoscope of claim 3, wherein, In step (2), the concentration of the aqueous solution of amino-functionalized multi-walled carbon nanotubes is 0.1-0.5 mg / mL.
7. The endoscope according to claim 3 or 6, characterized by In step (2), the oscillating treatment time is 5-6 hours.
8. The endoscope of claim 7, wherein, In step (2), the oscillating frequency in the horizontal shaker is 80-100 rpm.
9. The endoscope of claim 3, wherein, In step (2), before natural air-drying, washing with pure water is further included.