Brevundimonas vesicularis capable of degrading cephalexin and application thereof

Abstract

The invention relates to a blister-shaped Brevundimonas cefalexin-degradable bacterium and application thereof. The invention screens a blister-shaped Brevundimonas CEF1 strain which can tolerate high-concentration antibiotics and efficiently degrade cefalexin at high temperature. The concentration of cefalexin is 100-1000 mg / L, the inoculation amount of CEF1 is 1%-20%, the degradation temperature is 20-50 DEG C, and the pH is 5-9. The bacterium can effectively degrade cefalexin. Under the conditions of a temperature of 45 DEG C, a culture medium pH of 7, an inoculation amount of 10%, and a rotation speed of 200 r / min, the degradation rate of the strain CEF1 to 500 mg / L cefalexin reaches 89.65% in 20 hours, and under the condition of 50 DEG C, the strain can also degrade 80.87% of cefalexin. The strain has high biological safety and will not endanger human health or pollute the environment during application.

Description

Technical Field

[0001] The invention relates to the field of cephalexin degradation, and in particular to Brevundimonas vesicularis capable of degrading cephalexin and application thereof. Background Art

[0002] In recent years, due to the excessive use of antibiotics, they have accumulated and discharged continuously in the environment and caused serious pollution. Antibiotics have become an emerging pollutant and an important part of pharmaceuticals and personal care products (PPCPs). Antibiotic resistance has become a serious public health crisis that threatens the global social and economic development, causing serious harm to human health and the ecological environment. Antibiotics are a class of secondary metabolites produced by microorganisms during their growth, as well as chemically synthesized or semi-synthesized similar compounds that can selectively inhibit the growth and survival of other microorganisms. They have been widely used in medicine, agriculture, animal husbandry and other fields. According to different chemical structures, functions and modes of action, antibiotics can be divided into β-lactams, fluoroquinolones (FQs), macrolides (mLs), sulfonamides (SAs) and tetracyclines (TC). According to relevant statistics, from 2000 to 2015, the use of antibiotics in 76 countries increased from 21.1 billion doses to 34.8 billion doses. If they are not used rationally, it is expected to reach 126 billion doses by 2030. my country's antibiotic production and consumption are among the highest in the world. In 2013, my country's total antibiotic production reached 248,000 tons, and the consumption was 162,000 tons, accounting for about half of the world's total antibiotic production. my country has included antibiotics in the "List of New Pollutants under Key Control".

[0003] Cephalexin is a first-generation broad-spectrum β-lactam antibiotic with the advantages of broad antibacterial spectrum, few side effects, and strong bactericidal ability. It is widely used to treat human and animal diseases. The conversion rate of cephalexin in the organism is only 10%, and the remaining 90% is excreted through urine or feces. Therefore, with the excessive use of cephalexin, it persists in the environment and causes serious pollution, causing the accumulation and spread of biological toxicity and drug resistance genes (ARGs), posing a serious threat to human health and ecological security. Therefore, the treatment of cephalexin in the environment has become an urgent problem to be solved.

[0004] Microbial degradation is an efficient, environmentally friendly and economical method for treating antibiotics. At present, several studies on the degradation of cephalexin by microorganisms have been reported in the literature. Among them, two strains of Brevundimonas ce21 and ce22 that can degrade cephalexin were identified as Pseudomonas. In a culture medium with a cephalexin concentration of 1 mg / L, the degradation rates of cephalexin were 90% and 46.7% respectively; Rhizobium CLX-2 and Klebsiella CLX-3 could quickly remove 99% of cephalexin with an initial concentration of 10 mg / L within 12 hours; and Ochrobacterium spp. had a degradation rate of 100% after 36 hours of cultivation at a cephalexin concentration of 10 mg / L. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a Brevundimonas vesicularis capable of degrading cephalexin and its application in view of the above shortcomings.

[0006] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0007] A Brevundimonas bullata that can degrade cephalexin is Brevundimonas bullata CEF1, which was deposited in the General Microbiology Center of the China Culture Collection Administration on November 13, 2023, with the deposit number CGMCC No.28948.

[0008] The application of the Brevundimonas vesicularis capable of degrading cephalexin in degrading cephalexin.

[0009] Furthermore, the concentration of cephalexin during degradation is 100-1000 mg / L.

[0010] Furthermore, during the degradation, the inoculation amount of the cephalexin-degradable Brevundimonas vesicularis is 1%-20%.

[0011] Furthermore, the environmental temperature during degradation is 20-50°C.

[0012] Furthermore, the environmental pH during degradation is 5-9.

[0013] The invention has the following beneficial effects: experiments prove that the Brevundimonas bullata provided by the invention can tolerate high-concentration antibiotics and is a microbial strain that can efficiently degrade cephalexin at high temperature; the concentration of cephalexin is 100-1000 mg / L, the inoculation amount of CEF1 is 1%-20%; the degradation temperature is 20-50 DEG C, and the pH is 5-9, and cephalexin can be effectively degraded; under the conditions of a temperature of 45 DEG C, a culture medium pH of 7, an inoculation amount of 10%, and a rotation speed of 200 r / min, the degradation rate of 500 mg / L cephalexin by the strain CEF1 reaches 89.65% in 20 hours, and under the condition of 50 DEG C, 80.87% of cephalexin can be degraded; the strain has high biological safety, will not endanger human health or pollute the environment during application, and has important application value.

[0014] The present invention is described in detail below with reference to the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 is the relationship curve between the peak area and concentration of cephalexin;

[0016] Figure 2 is the growth curve of strain CEF1;

[0017] Figure 3 This is the colony morphology of strain CEF1 after growing on LB solid medium for 2 days;

[0018] Figure 4 It is a curve diagram showing the effect of temperature on the degradation of CEF1;

[0019] Figure 5 It is a curve diagram showing the effect of pH on the degradation of CEF1;

[0020] Figure 6 This is a curve diagram showing the effect of inoculation amount on the degradation effect of CEF1;

[0021] Figure 7 This is a curve diagram showing the effect of substrate concentration on CEF1 degradation. DETAILED DESCRIPTION

[0022] The following examples are provided for a better understanding of the present invention, but are not intended to limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The experimental materials used in the following examples are purchased from conventional biochemical reagent stores unless otherwise specified.

[0023] 1. Screening of Brevundimonas vesicularis strains capable of degrading cephalexin

[0024] 1. Material preparation

[0025] Sample source: coastal sediments from Dashentang, Tianjin

[0026] LB liquid medium: NaCl 10.0g, peptone 10.0g, yeast powder 5.0g, deionized water to 1L, use NaOH to adjust the pH to 7.0±0.2. On this basis, add 1.5% to 2% agar powder to make LB solid medium, sterilize at 121℃ for 20min.

[0027] Inorganic salt culture medium: composed of 0.5g (NH4) 2SO4, 0.5g KH2PO4, 1.5g K2HPO4, 0.15g MgSO4·7H2O, 0.5g NaCl and 1.0mL trace elements, supplemented to 1L with deionized water, sterilized at 121℃ for 20min. The trace elements used in this patent include 0.3g MnSO4·4H2O, 0.2g ZnSO4·7H2O, 0.02g (NH4) 6Mo7O2·4H2O, 0.1g CuSO4·5H2O, 0.5g CoCl2·6H2O, 0.05g CaCl2·2H2O, 0.5g FeSO4·7H2O.

[0028] 2. Preliminary screening of bacterial strains

[0029] Sediment samples were collected from the coastal area of ​​Dashentang, Tianjin. 5g of sludge was inoculated into 100mL of inorganic salt liquid culture medium. The final concentration of cephalexin in the culture medium was 50mg / L. The culture was continuously cultured in a constant temperature shaking incubator at 30℃ and 200r / min for 5 days. 5mL of culture solution was transferred to an inorganic salt culture medium with a cephalexin concentration of 100mg / L. After another 5 days of culture under the same conditions, the culture was transferred to a culture medium with a cephalexin concentration of 200mg / L. The culture was then cultured for 5 days. 5mL of culture solution was transferred to a culture medium with a cephalexin concentration of 500mg / L. The bacterial solution was then diluted 10 -1 , 10 -2 , 10 -3 , 10 -4 and 10 -5 The purified colonies were transferred to a liquid inorganic salt medium with cephalexin as the sole carbon source to screen strains with degradation performance.

[0030] 3. Strain rescreening

[0031] The strain obtained in the above steps is inoculated into LB liquid medium for activation culture, and then inoculated into an inorganic salt liquid medium with cephalexin as the sole carbon source at an inoculum of 5%, and cultured under shaking conditions of 30°C and 200r / min. The degradation rate of each strain is determined by high performance liquid chromatography, and the strain CEF1 that can efficiently degrade cephalexin is screened. At present, there is no report on the degradation of cephalexin by Brevundimonas vesicularis at home and abroad. Strain CEF1 is a new strain that can degrade cephalexin.

[0032] 4. Cephalexin content determination method

[0033] The bacterial culture solution was centrifuged at 800 rpm for 10 min. The precipitate was discarded, and the supernatant was retained and filtered through a 0.22 um microporous membrane, and then sealed for testing.

[0034] HPLC detection conditions: octadecylsilane bonded silica gel as filler; water-methanol-3.86% sodium acetate solution-4% acetic acid solution (742:240:15:3) as mobile phase; detection wavelength 254nm, column temperature 30°C. Substitute the measured peak area into the standard curve to calculate the substrate concentration and the degradation rate.

[0035] Drawing of the standard curve of cephalexin: Accurately weigh 300 mg of cephalexin in a 100 mL volumetric flask, dilute to the mark with mobile phase, and prepare a standard solution containing 10 mg of cephalexin per mL. Add mobile phase to dilute and prepare 50, 100, 200, 400, 600, 800, 1000, 1200, and 1400 mg / L of cephalexin standard solution. Draw the standard curve with the mass concentration of cephalexin as the horizontal axis and the peak area as the vertical axis. The curve drawn is as follows: Figure 1 As shown, Y = 12.464X-296.99, R 2 =0.9993, indicating a good linear relationship in the concentration range of 50-1000 mg / L.

[0036] 2. Growth curve of strain CEF1

[0037] Take the activated strain CEF and inoculate it into LB liquid culture medium. Under appropriate culture conditions, take the bacterial solution regularly and measure the OD value at a wavelength of 600nm. Within a certain range, the bacterial solution concentration and the optical density value are linearly related. Plot the measured OD value and its corresponding culture time to draw the growth curve of the bacteria, as shown in the attached figure. Figure 2 The specific steps are as follows:

[0038] Take the preserved bacteria, inoculate it into LB liquid culture medium, culture it at 30℃, 200r / min for 12h, and set aside. The components of LB liquid culture medium are: NaCl5.0g, peptone 10.0g, yeast powder 5.0g, deionized water to 1000mL, pH adjusted to 7.0±0.2, sterilized at 121℃ for 20min; take 1mL of bacterial solution and transfer it into 200mL LB liquid culture medium; place it in a constant temperature shaking incubator at 30℃, 200r / min, take 4mL of bacterial solution at 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24h, and immediately store it in the refrigerator. Finally, measure the OD value at a wavelength of 600nm to make a growth curve. Figure 2 It can be seen that strain CEF1 entered the logarithmic growth phase from 6h.

[0039] 3. Strain morphology and molecular biological identification

[0040] 1 Observation of colony morphology

[0041] The strain CEF1 was grown on LB solid medium for 2 days, and the colony morphology was as follows Figure 3 As shown, the colonies are white, round, and have smooth and moist edges.

[0042] 2 Molecular biological identification of strain CEF1

[0043] 2.1 Genome extraction

[0044] The extraction method of strain CEF1 genomic DNA is as follows:

[0045] (1) Collect 1.0×10 9 (1mL bacterial solution OD 600 1-1.5) of bacterial culture, centrifuge at 12000×g for 30s, discard the supernatant, and resuspend the precipitate with 150 μL of Buffer S to which RNase A has been added.

[0046] (2) Add 20 μL of lysozyme storage solution, mix well, and let stand at room temperature for 5 min.

[0047] (3) Add 30 μL 0.25 M EDTA (pH 8.0), mix well, and place on ice for 5 min.

[0048] (4) Add 450 μL Buffer GA, vortex for 15 seconds, and incubate in a 65°C water bath for 10 minutes.

[0049] (5) Add 400 μL of Buffer GB and 1 mL of Buffer DV (precooled at 4°C), mix vigorously, and centrifuge at 12,000 × g for 2 min.

[0050] (6) Discard the upper phase as much as possible and retain the interphase precipitate and lower phase. Add 1 mL of 4°C pre-cooled Buffer DV, mix vigorously, and centrifuge at 12,000 × g for 2 min.

[0051] (7) Discard the upper phase and transfer the lower phase to a filter (the filter is placed in a 2 mL centrifuge tube). Centrifuge at 12000×g for 1 min.

[0052] (8) Discard the filter and add 400 μL of Buffer BV to the filtrate and mix well.

[0053] (9) Place the preparation tube in a 2 mL centrifuge tube, transfer the mixed solution in step (8) into the preparation tube, and centrifuge at 12,000 × g for 1 min.

[0054] (10) Discard the filtrate, place the preparation tube back into the original 2 mL centrifuge tube, add 500 μL Buffer W1, and centrifuge at 12,000 × g for 1 min.

[0055] (11) Discard the filtrate, return the preparation tube to the original 2 mL centrifuge tube, add 700 μL Buffer W2, and centrifuge at 12,000 × g for 1 min.

[0056] (12) Wash once more with 700 μL Buffer W2 using the same method.

[0057] (13) Discard the filtrate, return the preparation tube to the original 2 mL centrifuge tube, and centrifuge at 12,000 × g for 1 min.

[0058] (14) Place the preparation tube in another clean 1.5 mL centrifuge tube, add 100-200 μL Eluent or deionized water to the center of the siLica membrane, and let it stand at room temperature for 1 min. Centrifuge at 12,000 × g for 1 min to elute the DNA.

[0059] 2.2 Primer sequence for bacterial genome PCR amplification: 27F: 5-AGAGTTTGATCCTGGCTCAG-3

[0060] 1492R:5-GGTTACCTTGTTACGACTT-3

[0061] PCR amplification reaction system

[0062] Add the following ingredients to a 0.2 mL centrifuge tube:

[0063] Table 1 PCR amplification reaction system

[0064]

[0065] Mix by flicking gently, collect the droplets on the tube wall to the bottom of the tube by instant centrifugation, and perform PCR reaction on a PCR amplification instrument. The reaction parameters are as follows:

[0066] Table 2 PCR amplification reaction program

[0067]

[0068] After the reaction was completed, 3uL of PCR product was taken for 1% agarose gel electrophoresis to confirm the PCR amplified fragment. The PCR product was recovered using the AxyPrep DNA gel recovery kit and then sent to Shanghai Paisono Co., Ltd. for 16S sequencing.

[0069] 2.3 Sequencing results

[0070] After sequencing, the length of the 16S rDNA gene sequence of strain CEF1 was 1457bp. Comparison in the NCBI database showed that the 16S rDNA sequence of strain CEF1 had the highest similarity with Brevundimonas bullata IAM 13153, reaching 99.01%. The strain was deposited in the General Microbiology Center of the China Microbiological Culture Collection Administration on November 13, 2023, with the deposit number CGMCCNO.28948. Deposit address: Institute of Microbiology, Chinese Academy of Sciences, No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing. The obtained 16S rDNA sequence is as follows:

[0071] CTGAGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCCT

[0072] AACACATGCAAGTCGAACGGACCCTTCGGGGTTAGTGGCGGACGGGT

[0073] GAGTAACACGTGGGAACGTGCCTTTAGGTTCGGAATAGCTCCTGGAAA

[0074] CGGGTGGTAATGCCGAATGTGCCCTTCGGGGGAAAGATTTATCGCCTTT

[0075] AGAGCGGCCCGCGTCTGATTAGCTAGTTGGTGAGGTAATGGCTCACCA

[0076] AGGCGACGATCAGTAGCTGGTCTGAGAGGATGACCAGCCACATTGGG

[0077] ACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTGGGGAATCT

[0078] TGCGCAATGGGCGAAAGCCTGACGCAGCCATGCCGCGTGAATGATGAA

[0079] GGTCTTAGGATTGTAAAATTCTTTCACCGGGGACGATAATGACGGTACC

[0080] CGGAGAAGAAGCCCCGGCTAACTTCGTGCCAGCAGCCGCGGTAATAC

[0081] GAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTAG

[0082] GCGGACATTTAAGTCAGGGGTGAAATCCCAGAGCTCAACTCTGGAACT

[0083] GCCTTTGATACTGGATGTCTTGAGTGTGAGAGAGGTATGTGGAACTCC

[0084] GAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAGTGGCGA

[0085] AGGCGACATACTGGCTCATTACTGACGCTGAGGCGCGAAAGCGTGGGG

[0086] AGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATT

[0087] GCTAGTTGTCGGGCTGCATGCAGTTCGGTGACGCAGCTAACGCATTAA

[0088] GCAATCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATT

[0089] GACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCA

[0090] ACGCGCAGAACCTTACCACCTTTTGACATGCCTGGACCGCCACGGAGA

[0091] CGTGGCTTTCCCTTCGGGGACTAGGACACAGGTGCTGCATGGCTGTCG

[0092] TCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAAC

[0093] CCTCGCCATTAGTTGCCATCATTTAGTTGGGAACTCTAATGGGACTGCC

[0094] GGTGCTAAGCCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCC

[0095] CTTACAGGGTGGGCTACACACGTGCTACAATGGCGACTACAGAGGGTT

[0096] AATCCTTAAAAGTCGTCTCAGTTCGGATTGTCCTCTGCAACTCGAGGG

[0097] CATGAAGTTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAAT

[0098] ACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTGGT

[0099] TCTACCCGAAGGCGATGCGCTAACCGTAAGGAGGCAGTCGACCACGGT

[0100] AGGGTCAGCGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAA

[0101] CCTGCGGCTGGATCACCTCCTTT

[0102] IV. Degradation characteristics of strain CEF1

[0103] 1. Effect of temperature on the degradation effect of CEF1

[0104] Take the above CEF1 strain, after activation in LB liquid medium, take 10% of the inoculation amount of bacterial liquid, centrifuge at 8000rpm for 2min, discard the supernatant LB medium, resuspend 3 times with sterile PBS solution to remove the residual LB medium, and then inoculate into a sterile inorganic salt medium with a concentration of 500mg / L cephalexin, the culture temperature is set to 20°C, 25°C, 30°C, 35°C, 40°C, 45°C and 50°C, the shaking speed is set to 200r / min, the pH is 7, three parallels are set for each group, and a sterile treatment is set as a control, and uniform culture is performed. Samples are taken after 20h to determine the residual concentration of cephalexin, and the degradation rate is calculated.

[0105] At different temperatures, due to the differences in the characteristics of the strains themselves, their degradation performance will also vary. Figure 4 It can be seen that the degradation of 500 mg / L cephalexin by strain CEF1 at different temperatures is significantly different. In the temperature range of 20-50°C, it can produce different degrees of degradation of cephalexin. As the culture temperature increases, the degradation ability of strain CEF1 gradually increases. When the culture temperature reaches 45°C, the degradation ability of the strain is the strongest, reaching 89.65%, and the degradation rate can reach 80.87% at 50°C, indicating that the strain can be used to degrade cephalexin in high temperature environments.

[0106] Effect of 2pH on CEF1 degradation

[0107] Take the above CEF1 strain, after activation in LB liquid medium, take 10% of the inoculation amount of bacterial liquid, centrifuge at 8000rpm for 2min, discard the supernatant LB medium, resuspend 3 times with sterile PBS solution to remove the residual LB medium, and then inoculate into a sterile inorganic salt medium with a concentration of 500mg / L cephalexin, the medium pH is set to 5, 6, 7, 8 and 9 respectively, the culture temperature is set to 35°C, the shaking speed is set to 200r / min, three parallels per group, and a sterile treatment is set as a control, and uniform culture is performed. Samples are taken after 20h to determine the residual concentration of cephalexin and calculate the degradation rate.

[0108] At different pH values, due to the differences in the characteristics of the strains themselves, their ability to degrade pollutants will also vary. Some bacteria can tolerate acid, some can tolerate alkali, and some can only degrade under neutral conditions. Figure 5It can be seen that the degradation effect of strain CEF1 is poor in a strong acidic environment. As the acidity weakens, the degradation effect gradually increases. At a pH of 8, the degradation rate reaches a maximum of 76.44%. Strain CEF1 is relatively more suitable for an alkaline environment. This may be because the microorganism produces organic acids in the process of degrading cephalexin, which causes the acidic substances in the degradation system to accumulate and the pH to drop rapidly, thereby inhibiting the degradation of cephalexin. However, under alkaline conditions, the organic acids finally generated by the strain will be neutralized by the base.

[0109] 3 Effect of inoculation amount on CEF1 degradation

[0110] Take the above CEF1 strain, after activation in LB liquid medium, take 1%, 2%, 5%, 10%, 15% and 20% of the inoculation amount of bacterial solution respectively, centrifuge at 8000rpm for 2min, discard the supernatant LB medium, resuspend 3 times with sterile PBS solution to remove the residual LB medium, and then inoculate into a sterile inorganic salt medium with a concentration of 500mg / L cephalexin, set the culture temperature to 35°C, pH to 7, set the shaking speed to 200r / min, three parallels per group, and set the sterile treatment as a control, and culture uniformly. Take samples after 20h to determine the residual concentration of cephalexin, and calculate the degradation rate.

[0111] The inoculum size can affect the initial growth of microorganisms. Too low an inoculum size will result in a longer delay period in bacterial culture, thus affecting the degradation rate. Figure 6 It can be seen that with the increase of inoculation amount, the degradation rate of cephalexin by strain CEF1 shows an upward trend. When the inoculation amount is 15%, the degradation effect of strain CEF1 is the best, and the degradation rate of 500 mg / L cephalexin can reach 86.55% after 20 hours of cultivation. When the inoculation amount exceeds 15%, the degradation rate shows a downward trend. The reason may be that the competitive inhibition between bacteria leads to insufficient nutrients, which in turn affects the degradation efficiency of the strain for cephalexin.

[0112] 4 Effect of substrate concentration on CEF1 degradation

[0113] Take the above CEF1 strain, after activation in LB liquid medium, take 10% of the inoculation amount of bacterial liquid, centrifuge at 8000rpm for 2min, discard the supernatant LB medium, resuspend 3 times with sterile PBS solution to remove the residual LB medium, and then inoculate into sterile inorganic salt medium with initial concentrations of 100, 200, 500 and 1000mg / L cephalexin respectively, set the culture temperature to 35°C, pH to 7, set the shaking speed to 200r / min, three parallels per group, and set the sterile treatment as a control, and culture uniformly. Take samples after 20h to determine the residual concentration of cephalexin, and calculate the degradation rate.

[0114] The degradation effect of strain CEF1 on cephalexin in inorganic salt liquid medium with initial concentrations of 100, 200, 500, and 1000 mg / L is as follows Figure 7 As shown. For strain CEF1, when the concentration is 100-200 mg / L, the degradation rate of cephalexin increases from 61.09% to 65.78%. However, when the concentration is too high, the degradation efficiency of cephalexin by strain CEF1 will gradually decrease. It can be seen that cephalexin is the only carbon source for the growth of the strain, and the concentration has a certain effect on the degradation effect of the strain. When the concentration is 1000 mg / L, CEF1 can still degrade 52.8% of cephalexin, indicating that the bacteria can tolerate high concentrations of cephalexin and can effectively degrade it.

[0115] The above is an example of the best implementation of the present invention, and the parts not described in detail are common knowledge of ordinary technicians in the field. The protection scope of the present invention shall be based on the content of the claims, and any equivalent transformation based on the technical enlightenment of the present invention is also within the protection scope of the present invention.

Claims

1. A Brevundimonas vesicularis for degrading cephalexin, characterized in that: The vesicular Brevundimonas is vesicular Brevundimonas ( Brevundimonas bullata )CEF1, deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on November 13, 2023, with the deposit number CGMCC No.28948.

2. Use of the cephalexin-degrading Brevundimonas vesicularis according to claim 1 in the degradation of cephalexin.

3. The use according to claim 2, characterized in that: The concentration of cephalexin during degradation was 100-1000 mg / L.

4. The use according to claim 2, characterized in that: During the degradation, the inoculation amount of the cephalexin-degrading Brevundimonas vesicularis is 1%-20%.

5. The use according to claim 2, characterized in that: The ambient temperature during degradation is 20-50°C.

6. The use according to claim 2, characterized in that: The environmental pH during degradation is 5-9.

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