A skin cell repair and anti-wrinkle composition and preparation method thereof

Abstract

The present invention discloses a skin cell repair anti-wrinkle composition and a preparation method thereof, which specifically belongs to the technical field of skin care products. The skin cell repair anti-wrinkle composition includes ingredients including skin conditioning components, composite functional oils and emulsifiers, wherein the skin conditioning components are sea fennel extract, unicorn extract, oligopeptide-1, sea red bean extract, cape aloe leaf extract and ebony bark extract, and the composite functional oils are brown-spotted pit viper oil and black tea seed oil; the repair anti-wrinkle composition can effectively promote collagen I synthesis and effectively inhibit the activity of elastase; the resident skin topical preparation provided by the present invention has excellent firming and anti-wrinkle effects, and the skin topical preparation has a high lactic acid stinging improvement rate and has a good repair effect.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care products, and specifically relates to a skin cell repair and anti-wrinkle composition and a preparation method thereof. Background Art

[0002] Skin health issues are attracting more and more attention. As the largest organ in the human body, the health of the skin is directly related to a person's overall image and physical and mental health. However, under the influence of internal and external factors, the skin often suffers from problems such as aging, dryness, and wrinkles, which seriously affect people's quality of life and self-confidence.

[0003] Skin aging is a complex process that involves the interaction of multiple factors. Among them, ultraviolet radiation, free radical damage, oxidative stress, etc. are all important factors that lead to skin aging. These factors can cause skin cell damage, collagen and elastic fiber reduction, skin elasticity decrease, and wrinkles. In addition, with age, the regeneration ability of skin cells will gradually weaken, making it more difficult for the skin to resist the damage of the external environment.

[0004] In order to improve the problem of skin aging, people have been looking for effective skin repair and anti-wrinkle methods. Traditional skin care methods can only temporarily improve the condition of the skin surface, but cannot fundamentally solve the problem of skin cell damage.

[0005] Therefore, developing a composition that can deeply repair skin cells and has significant anti-wrinkle effects is the research focus of this application. Summary of the invention

[0006] In view of the deficiencies in the prior art, the present invention aims to provide a skin cell repair and anti-wrinkle composition and a preparation method thereof. The composition comprises a variety of natural plant extracts and active ingredients, which can deeply penetrate the skin, repair damaged cells, promote the production of collagen and elastic fibers, and enhance skin elasticity and firmness.

[0007] To achieve the above object, the present invention discloses the following technical solutions:

[0008] In a first aspect, the present invention provides a skin cell repair and anti-wrinkle composition, comprising a skin conditioning component, a composite functional oil and an emulsifier;

[0009] The skin conditioning component is sea fennel extract, unicorn extract, oligopeptide-1, sea red bean extract, aloe vera leaf extract and ebony bark extract;

[0010] The composite functional oil is Agkistrodon acutus oil and Nigella sativa seed oil.

[0011] Furthermore, the skin conditioning component comprises the following components by mass:

[0012]

[0013] Furthermore, the composite functional oil comprises the following components by weight:

[0014] 2-5 parts of Pit Viper oil;

[0015] 7-9 parts of black tea seed oil.

[0016] Furthermore, the emulsifier is at least one of cetearyl alcohol, cetearyl glucoside, PEG-100 stearate, sodium lauryl sulfate and glyceryl stearate.

[0017] Sea fennel extract has the effects of anti-aging, promoting skin metabolism, improving overall skin tone, and maintaining the water-oil balance of the skin. At the same time, it can also whiten the skin, fade spots, improve melanin, and make the skin white and shiny;

[0018] Kirin extract can induce endothelial cell migration, capillary proliferation, promote fibroblast proliferation, effectively rebuild collagen fiber bundles, and restore skin elasticity and toughness;

[0019] Oligopeptide-1 is included in my country's "Catalogue of Used Cosmetic Raw Materials" (2015 edition). It is a synthetic peptide composed of three amino acids, including glycine, histidine, and lysine. It has anti-inflammatory, repairing, improving skin condition, anti-aging and promoting wound healing. It can promote skin metabolism and collagen regeneration, and help the skin restore elasticity and luster.

[0020] Sea red bean extract can resist ultraviolet rays in the sun, prevent sunburn and sun spots, and play a positive role in protecting the skin from ultraviolet damage;

[0021] Aloe vera leaf extract is rich in polysaccharides and natural moisturizing factors, which can provide long-lasting moisturizing effect for the skin, making it softer and smoother. At the same time, its antioxidant and anti-inflammatory effects help improve skin health and delay skin aging.

[0022] Ebony bark extract is mainly used as a skin conditioner, which can effectively help delicate skin increase elasticity and reduce skin wrinkles;

[0023] As an emollient and skin conditioner, Pit Viper oil has excellent dispersing, emulsifying and lubricating abilities, helping to improve the softness and smoothness of the skin;

[0024] Black tea seed oil is rich in unsaturated fatty acids, vitamins and antioxidants, which helps moisturize, nourish and anti-aging, and can enhance skin elasticity and radiance;

[0025] The present invention uses a combination of sea fennel extract, kelp extract, oligopeptide-1, sea red bean extract, aloe vera leaf extract, ebony bark extract, brown-spotted pit viper oil and black tea seed oil to comprehensively improve skin conditions and enhance the skin's firming, anti-wrinkle, anti-aging and repair capabilities. The sea fennel extract, kelp extract and oligopeptide-1 promote skin metabolism and collagen regeneration, and enhance the anti-aging effect; the aloe vera leaf extract, ebony bark extract and sea red bean extract promote each other to enhance the regeneration and repair ability of skin cells; and the black tea seed oil and brown-spotted pit viper oil are rich in unsaturated fatty acids, and the two promote skin metabolism under the synergistic effect, so that skin cells grow and divide faster, and the elasticity and smoothness of the skin are improved.

[0026] In a second aspect, the present invention provides a repairing and anti-wrinkle leave-on skin external preparation, characterized in that it comprises the composition described in the first aspect.

[0027] Furthermore, the amount of the composition added to the leave-on skin topical preparation is ≥5% by mass.

[0028] Furthermore, it also includes a thickener, a chelating agent, an antioxidant, a preservative, a moisturizer, a pH adjuster and a solvent.

[0029] Furthermore, the antioxidant is at least one of tocopherol acetate, tocopherol, ascorbic acid, and propyl gallate;

[0030] The preservative is at least one of p-hydroxyacetophenone, phenoxyethanol, and ethylhexylglycerin;

[0031] The moisturizing agent is at least one of glycerin, allantoin, butylene glycol, and 1,2-hexanediol;

[0032] The pH regulator is at least one of arginine, sodium hydroxide and citric acid.

[0033] In a third aspect, the present invention provides a method for preparing the residual skin external preparation for repairing and anti-wrinkle according to the second aspect, characterized in that it comprises the following steps:

[0034] Step (1) taking a certain amount of thickener, chelating agent, antioxidant, preservative, humectant and solvent, heating to 65-85° C. to dissolve, stirring, homogenizing and mixing, and keeping warm for 20-60 minutes to obtain a mixture;

[0035] Step (2) cooling the mixture of step (1) to 40-50° C., adding the skin cell repair and anti-wrinkle composition and the pH regulator, dissolving, stirring and mixing, to obtain the repair and anti-wrinkle resident skin external preparation.

[0036] In a fourth aspect, the present invention provides the use of the skin cell repair and anti-wrinkle composition described in the first aspect in the preparation of a skin care product preparation having anti-wrinkle, repair, soothing, whitening and / or acne-removing effects.

[0037] Beneficial effects of the present invention:

[0038] 1. The skin cell repair and anti-wrinkle composition provided by the present invention can effectively promote the synthesis of Collagen I;

[0039] 2. The skin cell repair and anti-wrinkle composition provided by the present invention can effectively inhibit the activity of elastase;

[0040] 3. The sea fennel extract, unicorn extract, oligopeptide-1, sea red bean extract, aloe vera leaf extract, ebony bark extract, brown-spotted pit viper oil and black tea seed oil in the skin cell repair and anti-wrinkle composition provided by the present invention are indispensable, and the ingredients have a synergistic effect;

[0041] 4. The leave-on skin topical preparation provided by the present invention has excellent firming and anti-wrinkle effects. At the same time, the skin topical preparation has a high improvement rate of lactic acid stinging and has a good repair effect. DETAILED DESCRIPTION

[0042] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. It should be understood by those skilled in the art that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0043] Unless otherwise specified, the test methods used in the specific implementation methods are all conventional methods; the materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels; the percentages mentioned in the specific implementation methods, unless otherwise specified, are all mass percentages.

[0044] In the present invention:

[0045] The sea fennel extract is sea fennel (CRITHMUM MARITIMUM) extract, INCI name / English name is CRITHMUM MARITIMUM EXTRACT, purchased from Xi'an Jiatian Biotechnology Co., Ltd.

[0046] The DAEMONOROPSDRACO extract is a DAEMONOROPSDRACO extract, with the INCI name / English name being DAEMONOROPSDRACO EXTRACT, purchased from Xi'an Jiatian Biotechnology Co., Ltd.

[0047] The Adenanthera Pavonina extract is an Adenanthera Pavonina extract, with the INCI name / English name being ADENANTHERA PAVONINA EXTRACT, purchased from Xi'an Jiatian Biotechnology Co., Ltd.

[0048] Aloe barbadensis leaf extract is Aloe barbadensis leaf extract (ALOE FEROX), INCI name / English name is ALOE FEROX LEAF EXTRACT, purchased from Xi'an Jiatian Biotechnology Co., Ltd.

[0049] The ebony bark extract is ebony (DIOSPYROSEBENUM) bark extract, INCI name / English name is DIOSPYROSEBENUMBARKEXTRACT, purchased from Ningxia Vanilla Biotechnology Co., Ltd.

[0050] Agkitrodon halysblom Hoffi oil, INCI name / English name MAMUSHIOIL, purchased from Poli Aromatic Pharmaceutical Technology (Shanghai) Co., Ltd.

[0051] Black currant seed oil is black currant seed oil, INCI name / English name is RIBESNIGRUM (BLACKCURRANT) SEEDOIL, purchased from Poli Aromatic Pharmaceutical Technology (Shanghai) Co., Ltd.

[0052] Aloe barbadensis leaf extract is Aloe barbadensis leaf extract, INCI name / English name is ALOEBARBADENSISLEAFEXTRACT, purchased from Xi'an Jiatian Biotechnology Co., Ltd.

[0053] Other raw materials are commercially available.

[0054] Preparation of skin cell repair and anti-wrinkle composition

[0055] Weigh the raw materials according to Table 1, then add ionized water according to the concentrations in Tables 2 and 3, mix and disperse them uniformly to obtain a composite sample.

[0056] Table 1

[0057] Raw material name Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Sea fennel extract 2 3 3 3 - Kirin Extract 3 1 1 1 1 Oligopeptide-1 2.4 2.0 2.0 2.0 2.0 Sea bean extract 13 15 15 15 15 Aloe Barbadensis Leaf Extract 25 30 30 30 30 Aloe Barbadensis Leaf Extract - - - - - Ebony Bark Extract 0.05 0.1 0.1 - 0.1 Pit Viper Oil 5 2 4 2 2 Black tea seed oil 7 9 9 9 9 Cetearyl Alcohol 1.0 1.5 - 1.5 1.5 Cetearyl Glucoside - - 0.5 - - PEG-100 Stearate 0.5 - - - - Sodium Lauryl Sulfate - - 0.5 - - Glyceryl Stearate - - 0.5 - -

[0058] Table 1 (continued)

[0059]

[0060]

[0061] Note: “-” in the table means not added.

[0062] Table 2 Addition concentration of composition 1-3 (mass percentage / %)

[0063]

[0064] Table 3 Addition concentration of composition 4-11 (mass percentage / %)

[0065] Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Sample 11 Composition 4 5％ - - - - - - - Composition 5 - 5％ - - - - - - Composition 6 - - 5％ - - - - - Composition 7 - - - 5％ - - - - Composition 8 - - - - 5％ - - - Composition 9 - - - - - 5％ - - Composition 10 - - - - - - 5％ - Composition 11 - - - - - - - 5％ Deionized water Replenish to 100% Replenish to 100% Replenish to 100% Replenish to 100% Replenish to 100% Replenish to 100% Replenish to 100% Replenish to 100%

[0066] Note: “-” in the table means not added.

[0067] Composition performance test

[0068] Test of the synthetic ability of Collagen I

[0069] Type I collagen (Collagen I) is the most abundant collagen in the skin. Stimulating the synthesis of Collagen I by testing the composition is an important indicator of firming and anti-wrinkle testing.

[0070] Experimental reagents: DMEM (Gibco), fetal bovine serum (Zhongqiao Xinzhou), collagen I ELISA kit (Wuhan Huamei), and the composition samples prepared by samples 1-3 and samples 4-11.

[0071] The experimental steps are as follows:

[0072] (1) Cell culture: Prepare cell suspension 24 h before the experiment and inoculate the cell suspension into a 96-well cell culture plate, with 0.1 mL per well and 5000 cells per well, and culture for 24 h;

[0073] (2) Exposure: discard the original culture medium in the wells, add 0.1 mL of composition samples 1-11 to each well, add an equal amount of new culture medium to the blank control, and incubate in an incubator for 24 h;

[0074] (3) Sample collection: centrifuge and take the supernatant for fresh use or store at -20°C for later use;

[0075] (4) ELISA test: The absorbance value was measured at a wavelength of 450 nm using an ELISA reader, and the collagen I content in the sample was calculated using the standard curve method.

[0076] The experimental results are shown in Tables 4 and 5:

[0077] Table 4 Relative content of collagen I (%)

[0078]

[0079] Table 5 Relative content of collagen I (%)

[0080]

[0081] Result analysis:

[0082] The test results show that the skin cell repair and anti-wrinkle composition of samples 1-3 can effectively promote the synthesis of Collagen I. Among them, the ability of sample 3 to promote the synthesis of Collagen I is stronger than that of samples 1 and 2, indicating that the addition ratio of Agkistrodon acutus oil and black tea seed oil at 4:9 has a promoting effect on the firming and anti-wrinkle effect of the overall formula, so the addition ratio of Agkistrodon acutus oil and black tea seed oil is preferably 4:9;

[0083] At the same concentration, the firming and anti-wrinkle effects of samples 1-3 are better than those of samples 4-11, indicating that the sea fennel extract, unicorn extract, oligopeptide-1, red bean extract, aloe barbadensis leaf extract, ebony bark extract, pit viper oil and black tea seed oil in the composition are indispensable and have a synergistic effect.

[0084] Elastase activity inhibition test

[0085] Experimental principle: Collagen forms collagen fibers and reticular fibers, and elastin forms elastic fibers. These three fibers together form the connective tissue of the skin, which makes the skin plump and elastic. Elastase has the ability to decompose collagen, elastin and other proteins. The change in elastin structure is closely related to skin aging. The inhibitory effect of the test sample on the catalysis of elastase and its substrate is detected by an enzyme marker to determine whether the test sample has the ability to inhibit elastase.

[0086] The test steps are as follows:

[0087] (1) Reagent preparation

[0088] Preparation of 0.2mol / L Tris-HCl buffer at pH 8.0: Solution A: Accurately weigh 4.8456g of Tris particles, add 100mL of deionized water, stir and mix to dissolve to obtain 0.4mol / L Tris solution; Solution B: Take 3.34mL of concentrated hydrochloric acid and add 96.66mL of deionized water to dilute to obtain 0.4mol / L HCl solution; Take 100ml of Solution A and 44.76mL of Solution B, mix, adjust pH to 8.0, and add deionized water to make up to 200ml.

[0089] Elastase solution: Prepared with pH 8.0 Tris-HCL buffer, take 0.033g of elastase, add 10mL pH 8.0 Tris-HCL buffer to prepare 100U / mL elastase solution, prepare immediately before use; Substrate solution: weigh 1.8057mg N-succinyl-alanine-alanine-alanine-p-nitroaniline, dissolve in 10mL pH 8.0 Tris-HCl buffer;

[0090] Samples: Samples 1-3 and Samples 4-11.

[0091] (2) Reagent addition is shown in Table 6.

[0092] Table 6 Reaction solution composition (μL)

[0093] sample Enzyme solution Substrate Buffer blank / 50 100 50 Blank control / 50 / 150 sample 50 50 100 / Sample control 50 50 / 100

[0094] Incubate at room temperature for 60 min, detect the absorbance at 410 nm using an enzyme reader, and record and save the data.

[0095] (3) Data analysis

[0096] Elastase inhibition rate (%) calculation formula:

[0097] Elastase inhibition rate (%) = [1-(OD A -OD B )] / (OD C -OD D )×100%;

[0098] In the above formula: OD A is the absorbance value of the sample group (containing enzyme and substrate); OD B is the absorbance value of the sample control group (containing enzyme but not substrate); OD C is the absorbance value of the blank group (containing enzyme, substrate and no sample); OD D It is the absorbance value of the blank control group (containing enzyme but no substrate or sample).

[0099] The experimental results are shown in Tables 7 and 8:

[0100] Table 7 Elastase inhibition rate (%)

[0101]

[0102] Table 8 Elastase inhibition rate (%)

[0103]

[0104] Result analysis:

[0105] The test results show that the skin cell repair and anti-wrinkle composition of samples 1-3 can effectively inhibit the activity of elastase. Among them, at the same concentration, the firming and anti-wrinkle effect of samples 1-3 is better than that of samples 4-11, indicating that the sea fennel extract, unicorn extract, oligopeptide-1, sea red bean extract, aloe vera leaf extract, ebony bark extract, brown-spotted pit viper oil and black tea seed oil in the composition are indispensable and have synergistic effects.

[0106] Preparation of leave-on skin topical preparations

[0107] Step (1), weigh a certain amount of VP / DMAPA acrylic acid (ester) copolymer, glycerol, allantoin and deionized water according to Table 9, mix and stir, heat to 85° C., stir and homogenize until completely dissolved, keep warm for 20 minutes, and obtain an oil phase for use;

[0108] Step (2), weighing a certain amount of butanediol, 1,2-hexanediol, preservative and antioxidant, adding distilled water to dissolve, to obtain an aqueous phase; cooling the oil phase to 65° C., adding the pre-dissolved aqueous phase, and homogenizing and dispersing until completely mixed, to obtain a mixed phase;

[0109] Step (3), cooling the mixed phase to 45°C, adding the components of the composition prepared above and the pH adjuster in sequence, and stirring and dispersing them homogeneously until they are completely dissolved;

[0110] Step (4) Cooling to 38° C., discharging the material, and obtaining the residual skin external preparation Examples 1-11.

[0111] Table 9 Raw materials for preparation of leave-on skin topical preparations

[0112]

[0113] Note: Blank spaces in the table indicate that nothing is added.

[0114] Skin topical agent performance test

[0115] Human skin patch test

[0116] The above-prepared Examples 1-11 were subjected to the human skin patch test in reference to the "2022 Cosmetic Safety Technical Specifications".

[0117] The skin reaction was observed according to the standard at 30 minutes (after the indentation disappears), 24 hours and 48 hours, and the observation results were recorded (see Table 10).

[0118] Table 10 Human safety test results

[0119]

[0120]

[0121] The results of human skin patch tests showed that no adverse skin reactions occurred among 30 people.

[0122] Firming and anti-wrinkle human efficacy test

[0123] Experimental method: 55 people with facial wrinkles (30-45 years old) were selected as volunteers for the firming and anti-wrinkle efficacy test (every 5 volunteers used the same test sample). The volunteers used the leave-on skin topical preparation of Examples 1-11 on half of their faces, about 2 mL / time, once in the morning and evening every day, and the other half of their faces were not used as a control group. The change rates of their skin firmness R0 and skin elasticity R2 were measured at the 0th and 2nd weeks of use, respectively, to evaluate the effect of the leave-on skin topical preparation on reducing skin wrinkles.

[0124] Test instrument: Skin elasticity tester MPA580 (produced by CK Company of Germany).

[0125] The higher the skin firmness R0 value, the firmer the skin is; the higher the skin elasticity R2 value, the better the skin elasticity is.

[0126] Skin firmness R0 change rate = (skin firmness R0 value after 2 weeks of use - skin firmness R0 value before use) / skin firmness R0 value before use × 100%.

[0127] Skin elasticity R2 change rate = (skin elasticity R2 value after 2 weeks of use - skin elasticity R2 value before use) / skin elasticity R2 value before use × 100%.

[0128] The test results are shown in Table 11.

[0129] Table 11 Test results of firming and anti-wrinkle performance

[0130] Test samples Skin firmness R0 change rate (%) Skin elasticity R2 change rate (%) Control group -1.63 -1.02 Example 1 26.52 30.01 Example 2 24.13 29.15 Example 3 28.14 31.42 Example 4 12.11 13.52 Example 5 13.04 14.46 Example 6 10.22 11.15 Example 7 13.07 14.21 Example 8 16.23 16.98 Example 9 14.78 15.43 Example 10 18.48 19.51 Embodiment 11 14.01 14.96

[0131] Repair performance test

[0132] Volunteer screening: 60 people with sensitive skin (25-45 years old) were selected as volunteers for the repair performance test (every 5 volunteers used the same test sample).

[0133] Test steps: The test was conducted on the nasolabial folds of volunteers. 50ul of 10% lactic acid solution was dropped on a single-layer filter paper with a diameter of 0.8cm. The subjects were asked about the tingling sensation at 0.5min and 2min respectively. The pain was scored according to the 4-point system (0 for no tingling, 1 for mild tingling, 2 for moderate tingling, and 3 for severe tingling). If the subject reached 3 points at any time during the test, the test was terminated.

[0134] Sample use: Volunteers used the leave-on skin topical preparations of Examples 1-11 on their entire faces, and volunteers in the blank control group used deionized water as samples. Volunteers applied the product twice a day, morning and evening, about 1 mL, for two consecutive weeks, and repeated the above test steps after two weeks of use.

[0135] The lactic acid stinging scores of the volunteers before use and after 2 weeks of use were recorded, and the lactic acid stinging improvement rate of the volunteers was calculated. Wherein, lactic acid stinging improvement rate (%) = (average lactic acid stinging score before use - average lactic acid stinging score after 2 weeks of use) / average lactic acid stinging score before use × 100%; average lactic acid stinging score = average stinging score at 0.5 min + average stinging score at 2 min.

[0136] The higher the improvement rate of lactic acid stinging, the better the repair effect of the sample.

[0137] Table 12 Lactic acid stinging improvement rate

[0138] Test samples Lactic acid stinging improvement rate (%) Blank control group 1.74 Example 1 51.31 Example 2 50.62 Example 3 52.44 Example 4 23.62 Example 5 24.48 Example 6 21.07 Example 7 24.15 Example 8 30.18 Example 9 26.74 Example 10 21.25 Embodiment 11 16.34

[0139] Result analysis:

[0140] The test results in Tables 11 and 12 show that the resident skin topical preparations of Examples 1-3 have excellent firming and anti-wrinkle effects, and the improvement rate of lactic acid stinging of the skin topical preparations is high, and they have good repairing effects. Among them, the firming and anti-wrinkle effects and repairing effects of Examples 1-3 are better than those of Examples 4-11, indicating that the components in the composition provided by the present invention have a synergistic effect, and the lack of any one of the components will have a certain impact on the firming, anti-wrinkle and repairing effects of the overall formula.

[0141] Although the specific embodiments of the present invention are described above, it should be understood by those skilled in the art that this is only for illustration and the protection scope of the present invention is defined by the appended claims. Those skilled in the art may make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but these changes and modifications all fall within the protection scope of the present invention.

Claims

1. A skin cell repair and anti-wrinkle composition, characterized in that: The ingredients include skin conditioning components, compound functional oils and emulsifiers; The skin conditioning component comprises the following components in parts by weight: The composite functional oil comprises the following components in parts by weight: 2-5 parts of Pit Viper oil; 7-9 parts of black tea seed oil; The emulsifier is at least one of cetearyl alcohol, cetearyl glucoside, PEG-100 stearate, sodium lauryl sulfate and glyceryl stearate.

2. A residual skin external preparation for repairing and anti-wrinkle, characterized in that: Comprising the composition according to claim 1; Calculated by mass percentage, the addition amount of the composition in the leave-on skin external preparation is ≥5%.

3. The anti-wrinkle leave-on skin preparation according to claim 2, characterized in that: Also included are thickeners, chelating agents, antioxidants, preservatives, humectants, pH adjusters and solvents.

4. The leave-on skin external preparation for repairing and anti-wrinkle according to claim 3, characterized in that: The antioxidant is at least one of tocopherol acetate, tocopherol, ascorbic acid, and propyl gallate; The preservative is at least one of p-hydroxyacetophenone, phenoxyethanol, and ethylhexylglycerin; The moisturizing agent is at least one of glycerin, allantoin, butylene glycol, and 1,2-hexanediol; The pH regulator is at least one of arginine, sodium hydroxide and citric acid.

5. The method for preparing the anti-wrinkle residual skin preparation according to any one of claims 2 to 4, characterized in that: The following steps are involved: Step (1) taking a certain amount of thickener, chelating agent, antioxidant, preservative, humectant and solvent, heating to 65-85° C. to dissolve, stirring, homogenizing and mixing, and keeping warm for 20-60 minutes to obtain a mixture; Step (2) cooling the mixture of step (1) to 40-50° C., adding the skin cell repair and anti-wrinkle composition and the pH regulator, dissolving, stirring and mixing, to obtain the repair and anti-wrinkle resident skin external preparation.

6. Use of the skin cell repair and anti-wrinkle composition according to claim 1 in the preparation of skin care products with anti-wrinkle, repair, soothing, whitening and / or acne-removing effects.

Images