A Chinese medicine composition for improving microcirculation disorders and its preparation method and application

Abstract

The present invention relates to the field of medical technology, and in particular to a Chinese medicine composition for improving microcirculation disorders, and a preparation method and application thereof. The Chinese medicine composition for improving microcirculation disorders of the present invention comprises the following raw materials in parts by weight: 1-10 parts of Gastrodia elata, 1-5 parts of Panax notoginseng, 1-10 parts of lotus seed heart, and 5-30 parts of corn silk. The formula is scientific and reasonable, the process is simple, the taste is good, the cost is low, it is safe and effective, it can improve microcirculation disorders, reduce vascular endothelial damage, and has a good therapeutic effect on diseases such as cardiovascular and cerebrovascular diseases. The Chinese medicine composition can be applied to the preparation of a medicine for treating microcirculation disorders, and its main component is the same source of medicine and food, with relatively few side effects, suitable for long-term use, and is particularly important for the management of chronic diseases.

Description

Technical Field

[0001] The present invention relates to the field of medical technology, and in particular to a Chinese medicine composition for improving microcirculation disorders, and a preparation method and application thereof. Background Art

[0002] Microcirculation is the blood circulation in the capillaries between arterioles and venules. It is the most basic structural and functional unit in the circulatory system and plays an important role in providing oxygen and nutrients, transferring energy, exchanging information, and eliminating carbon dioxide and metabolic waste. Microcirculatory disorders are changes in the physical and chemical properties of blood, which cause the lumen to narrow, blood flow to slow down or thrombosis to form, causing local tissue ischemia, hypoxia and even necrosis, causing a series of clinical symptoms. Microcirculatory disorders are the common pathological basis of many diseases. Studies have shown that the occurrence and development of aging, tumors, hypertension, diabetes and many cardiovascular and cerebrovascular diseases are closely related to microcirculatory disorders. A sound microcirculatory function is the primary prerequisite for ensuring the normal functions of important organs in the body, and improving microcirculatory disorders has therefore received increasing attention.

[0003] At present, the commonly used drugs for improving microcirculation mainly include: microcirculation is the blood circulation in the capillaries between arterioles and venules, which is the most basic structural and functional unit in the circulatory system, vascular protection drugs, anticoagulants, thrombolytic and defibrillating drugs, antiplatelet drugs, vasodilators, blood viscosity reducing drugs, etc. Although they can improve microcirculatory disorders to some extent, they often have more toxic side effects. Traditional Chinese medicine can regulate the whole body through multiple targets and multiple pathways, which is superior in comparison. Summary of the invention

[0004] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a traditional Chinese medicine composition for improving microcirculation disorders. The traditional Chinese medicine composition is composed of Gastrodia elata, Panax notoginseng, lotus seed core and corn silk, which has the effect of improving microcirculation disorders and protecting microvessels.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] In a first aspect, the present invention provides a traditional Chinese medicine composition for improving microcirculation disorders, comprising the following components in parts by weight: 1-10 parts of Gastrodia elata, 1-5 parts of Panax notoginseng, 1-10 parts of lotus seed core, and 5-30 parts of corn silk.

[0007] Lotus coix, bitter and cold, can purge fire, and treat symptoms of restlessness and heat. Because it is an embryo, it has vitality, and it has the property of circulation in purging fire. Cardiovascular diseases are caused by qi stagnation and fire, and lotus seed heart leads the heart fire downward, clearing the heart and eliminating troubles; Gastrodia elata is a top grade in the "Nervous Materia Medica", with a pungent taste and warm. It can kill ghosts, poisons, bad air, eliminate carbuncle, lower limb hernia, and bleeding. Long-term use can increase qi and strength, grow fat and healthy, and lighten the body and increase age. Gastrodia elata can calm liver yang, dredge meridians and activate collaterals. Wood generates fire, calms the liver to suppress the hyperactivity of heart fire, and the internal burning of phase fire, which can reduce cardiovascular pressure; most cardiovascular diseases are caused by qi stagnation and blood stasis. Panax notoginseng can stop bleeding, dredge meridians and remove blood stasis, break all blood stasis, stop all new blood, and protect heart blood while removing blood stasis; corn silk is sweet and slightly warm. It enters the Yangming stomach meridian, relaxes the intestines and relieves gas, treats red and swollen breast nodules in women, has the effect of clearing away heat and promoting the production of body fluid, and has a good therapeutic effect on high blood sugar caused by yin deficiency and heat. The present invention combines four traditional Chinese medicines, and within the above range, the effective ingredients of the traditional Chinese medicines interact with each other, which has the effects of purging and tonifying, clearing away heat and relieving restlessness, promoting blood circulation and removing blood stasis, while not forgetting to promote the production of body fluid and tonify deficiency, and protecting cardiovascular and cerebrovascular vessels.

[0008] Preferably, the Chinese medicine composition for improving microcirculation disorders comprises the following components in parts by weight: 9 parts of Gastrodia elata, 3 parts of Panax notoginseng, 9 parts of lotus seed core, and 30 parts of corn silk.

[0009] In a second aspect, the present invention provides a method for preparing the above-mentioned Chinese medicine composition for improving microcirculation disorders, comprising the following steps:

[0010] (1) Weighing medicinal materials according to weight ratio, mixing, soaking in water, heating and steaming, filtering, and obtaining an extract A and a filter residue;

[0011] (2) taking the filter residue, adding water and boiling it, and filtering it to obtain extract B;

[0012] (3) The extract A and the extract B are mixed, allowed to stand, filtered, and concentrated to obtain the Chinese medicine composition for improving microcirculation disorders.

[0013] The invention uses secondary water extraction to fully dissolve and extract the effective ingredients contained in the medicinal raw materials, thereby maximizing the effect of the medicinal raw materials.

[0014] Preferably, in step (1), the amount of water added is 8-30 times the total weight of the medicinal material raw materials; and the cooking time is 30-45 minutes.

[0015] Preferably, in step (1), the soaking time is 0.5-3h.

[0016] Preferably, in step (2), the cooking time is 20 min-2 h.

[0017] Preferably, in step (3), the concentration is carried out by vacuum distillation.

[0018] Preferably, in step (3), the mixture is concentrated to a clear paste with a relative density of 1.0-1.2.

[0019] In a third aspect, the present invention provides the use of the above-mentioned Chinese medicine composition for improving microcirculation disorders in the preparation of a medicine for treating microcirculation disorders.

[0020] In a fourth aspect, the present invention provides a medicine for treating microcirculation disorders, including the above-mentioned traditional Chinese medicine composition for improving microcirculation disorders.

[0021] Preferably, the dosage form of the drug includes tablets, capsules, syrups, mixtures, and oral liquids.

[0022] Preferably, when the dosage form is a tablet or a capsule, the drug further comprises at least one of a diluent, a wetting agent, a binder, a disintegrant, and a lubricant.

[0023] Preferably, when the dosage form is a syrup or an oral solution, the drug further comprises at least one of a flavoring agent, a preservative, and a solubilizing agent.

[0024] Preferably, when the dosage form is a mixture, the medicine further comprises at least one of a flavoring agent and a preservative.

[0025] The beneficial effects of the present invention are:

[0026] The Chinese medicine composition for improving microcirculation disorder prepared by the present invention has a scientific and reasonable formula, simple process, good taste, low cost, safety and effectiveness, can improve microcirculation disorder, reduce vascular endothelial damage, and has a good therapeutic effect on cardiovascular and cerebrovascular diseases and the like.

[0027] Patients with chronic diseases usually need to take medication continuously. The Chinese medicine composition for improving microcirculation disorders described in the present invention is used to prepare drugs for treating microcirculation disorders. Its main ingredients are both medicinal and edible, with relatively few side effects, and it is suitable for long-term use, which is particularly important for the management of chronic diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The results of the improvement of the Chinese medicine composition on the endothelial injury of the mouse heart microvessels; A, the expression level of ICAM-1 in the heart vessels of each group of mice; B, the expression level of ZO-1 in the heart vessels of each group of mice; Among them, VWF is a vascular marker; * represents the significant difference between each group and the model group, ** represents P<0.01, and *** represents P<0.001.

[0029] Figure 2 The effect of the Chinese medicine composition on peripheral blood circulation in mice; A, representative images of blood flow imaging of both feet of mice in each group; B, statistical graph of blood flow perfusion ratio of right foot of mice in each group; * represents significant difference between each group and the model group, ** represents P<0.01, *** represents P<0.001.

[0030] Figure 3 Results of the improvement of mouse cardiac microvascular endothelial injury caused by intervention with a traditional Chinese medicine composition; A. Expression levels of VCAM-1 in the cardiac blood vessels of each group of mice; B. Expression levels of ZO-1 in the cardiac blood vessels of each group of mice; * represents significant differences compared with the model group, * indicates p < 0.05, ** indicates P < 0.01, *** indicates P < 0.001, and CD31 is a blood vessel marker. Detailed implementation manners

[0031] To better illustrate the objectives, technical solutions, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0032] 6-week-old SPF-grade C57BL / 6 mice and 8-week-old SPF-grade C57BL / 6N mice were purchased from Guangdong Vital River Laboratory Animal Technology Co., Ltd., and the animal production license number is: SCXK(Yue)2022-0063.

[0033] The sources of the reagents and materials used in the present invention are as follows: L-NAME (Sigma aldrich, N5751); empagliflozin (Boehringer Ingelheim, Germany, 14202853284); high-fat feed (Huafukang, H10060); folic acid (Yuanye, B21487); homocysteine (Merck, H4628).

[0034] Example 1:

[0035] An example of the traditional Chinese medicine composition for improving microcirculation disorders according to the present invention; the traditional Chinese medicine composition in this example includes the following components by weight: Gastrodia elata 9g, Panax notoginseng 3g, Nelumbo nucifera Gaertn. F. germplasma 9g, and Stigma maydis 30g.

[0036] The preparation method of the traditional Chinese medicine composition for improving microcirculation disorders includes the following steps:

[0037] (1) Weigh the raw medicinal materials according to the weight ratio, mix them, add 1 L of water, soak for 0.5 h, heat and cook for 0.5 h, and filter to obtain extract A and filter residue;

[0038] (2) Take the filter residue, add 0.5 L of water, cook for 20 min, and filter to obtain extract B;

[0039] (3) Mix extract A and extract B, let it stand overnight, filter, and concentrate the obtained filtrate to a clear paste with a relative density of 1.2 by reduced pressure distillation to obtain the traditional Chinese medicine composition for improving microcirculation disorders.

[0040] Example 2:

[0041] An embodiment of the Chinese medicine composition for improving microcirculation disorders according to the present invention; the Chinese medicine composition described in this embodiment includes the following components by weight: 9g of Gastrodia elata, 1g of Panax notoginseng, 5g of lotus seed core, and 20g of corn silk.

[0042] The preparation method of the Chinese medicine composition for improving microcirculation disorder comprises the following steps:

[0043] (1) Weigh the raw materials of medicinal materials according to the weight ratio, add 1 L of water, soak for 0.5 h, heat and cook for 45 min, filter, and obtain extract A and filter residue;

[0044] (2) Take the filter residue, add 0.75 L of water and boil for 25 min, then filter to obtain extract B;

[0045] (3) The extract A and the extract B are mixed, allowed to stand overnight, filtered, and the filtrate obtained is concentrated by vacuum distillation to a clear paste with a relative density of 1.0, thereby obtaining the Chinese medicine composition for improving microcirculation disorders.

[0046] Embodiment 3:

[0047] An embodiment of the Chinese medicine composition for improving microcirculation disorders according to the present invention; the Chinese medicine composition described in this embodiment includes the following components by weight: 10g of Gastrodia elata, 1g of Panax notoginseng, 1g of lotus seed core, and 20g of corn silk.

[0048] The preparation method of the Chinese medicine composition for improving microcirculation disorder comprises the following steps:

[0049] (1) Weigh the raw materials of medicinal materials according to the weight ratio, add 1 L of water, soak for 0.5 h, heat and cook for 30 min, filter, and obtain extract A and filter residue;

[0050] (2) Take the filter residue, add 0.75 L of water and boil for 2 h, then filter to obtain extract B;

[0051] (3) The extract A and the extract B are mixed, allowed to stand overnight, filtered, and the filtrate obtained is concentrated by vacuum distillation to a clear paste with a relative density of 1.0, thereby obtaining the Chinese medicine composition for improving microcirculation disorders.

[0052] Embodiment 4:

[0053] An embodiment of the Chinese medicine composition for improving microcirculation disorders according to the present invention; the Chinese medicine composition described in this embodiment includes the following components by weight: 1g of Gastrodia elata, 5g of Panax notoginseng, 10g of lotus seed core, and 5g of corn silk.

[0054] The preparation method of the Chinese medicine composition for improving microcirculation disorder comprises the following steps:

[0055] (1) Weigh the raw materials of medicinal materials according to the weight ratio, add 1 L of water, soak for 3 hours, heat and cook for 45 minutes, filter, and obtain extract A and filter residue;

[0056] (2) Take the filter residue, add 0.75 L of water and boil for 25 min, then filter to obtain extract B;

[0057] (3) The extract A and the extract B are mixed, allowed to stand overnight, filtered, and the filtrate obtained is concentrated by vacuum distillation to a clear paste with a relative density of 1.0, thereby obtaining the Chinese medicine composition for improving microcirculation disorders.

[0058] Comparative Example 1

[0059] A comparative example of the Chinese medicine composition for improving microcirculation disorders of the present invention; the Chinese medicine composition in this comparative example comprises the following components by weight: 9g of Gastrodia elata, 3g of Panax notoginseng, and 9g of lotus seed core.

[0060] The preparation method of the Chinese medicine composition for improving microcirculation disorder is the same as that in Example 1.

[0061] Comparative Example 2

[0062] A comparative example of the Chinese medicine composition for improving microcirculation disorders of the present invention; the Chinese medicine composition in this comparative example includes the following components by weight: 11g of Gastrodia elata, 1g of Panax notoginseng, 11g of lotus seed core, and 4g of corn silk.

[0063] The preparation method of the Chinese medicine composition for improving microcirculation disorder is the same as that in Example 1.

[0064] Comparative Example 3

[0065] A comparative example of the Chinese medicine composition for improving microcirculation disorders of the present invention; the Chinese medicine composition in this comparative example includes the following components by weight: 5g of Gastrodia elata, 6g of Panax notoginseng, 11g of lotus seed core, and 35g of corn silk.

[0066] The preparation method of the Chinese medicine composition for improving microcirculation disorder is the same as that in Example 1.

[0067] Efficacy test 1:

[0068] Experimental samples: Chinese medicine compositions prepared in Examples 1-4 and Comparative Examples 1-3.

[0069] Experimental animal grouping: 8-week-old SPF-grade C57BL / 6N mice were purchased as experimental mice and randomly divided into a normal group, a model group, a Chinese medicine composition group of comparative examples 1-3, a low-dose group of the Chinese medicine composition of Example 1, a medium-dose group of the Chinese medicine composition of Example 1, a high-dose group of the Chinese medicine composition of Example 1, a Chinese medicine composition group of Examples 2-4, and a positive drug group (empagliflozin), with 6 mice in each group.

[0070] The mice in the normal group were given ordinary feed and ordinary drinking water, and the mice in the model group and the drug group were given a free diet of high-fat feed and drinking water containing L-NAME (0.5 g / L) every day. The low-dose group of the Chinese medicine composition in Example 1 was gavaged with 5.3 g of crude drug / kg every day from the beginning of modeling, the medium-dose group of the Chinese medicine composition in Example 1 was gavaged with 10.4 g of crude drug / kg every day, and the high-dose group of the Chinese medicine composition in Example 1 was gavaged with 20.9 g of crude drug / kg every day from the beginning of modeling. The positive drug group was gavaged with 10 mg / kg of empagliflozin every day for 10 consecutive weeks. The normal group and the model group were administered with the same volume of normal saline.

[0071] After continuous oral administration for 10 weeks, the mice were killed after anesthesia, and the mouse heart tissue was collected for frozen sectioning, and the sections were stained using immunofluorescence staining. First, the slices were taken out and rinsed with 0.02% PBST three times, then the slices were placed in acetone and fixed at -20°C for 20 minutes, then the slices were taken out, 0.02% PBST was added and allowed to stand for 3 minutes, and repeated 3 times. The excess liquid around the tissue was wiped with lens paper, and a circle of appropriate size was drawn around the tissue with an immunohistochemical pen, and the blocking solution was added for blocking at room temperature for 1 hour. The blocking solution was discarded, and the primary antibody (anti-VWF / anti-ICAM-1 / anti-ZO-1) was added, and incubated at 4°C overnight. The primary antibody was discarded, and 0.02% PBST was added and allowed to stand for 5 minutes, and repeated three times. The PBST was dried, and the fluorescent secondary antibody was added, and incubated at room temperature in the dark for 2 hours. Discard the secondary antibody, add 0.02% PBST and let stand for 5 min, repeat three times, wipe off the excess water around the tissue and the traces of immunohistochemistry pen, seal the slide in the dark, and take pictures under an immunofluorescence microscope.

[0072] Tight junction protein ZO-1 is one of the important proteins that characterize vascular endothelial function. When the vascular endothelium is damaged, the expression of ZO-1 will decrease. Intercellular adhesion molecule-1 (ICAM-1) is an important adhesion molecule that mediates adhesion reactions. ICAM-1 is expressed at low levels on resting vascular endothelial cells. When the blood vessels are damaged, the expression of ICAM-1 will be significantly upregulated. Figure 1 The results show that when mice receive a double blow of high-fat diet and L-NAME, endothelial damage will occur in the heart and blood vessels of the mice. Compared with the normal group, the expression of ICAM-1 in the heart microvascular endothelial of the model group mice increased significantly, and the expression of ZO-1 decreased significantly; the Chinese medicine composition of Examples 1-4 and the positive drug group can improve vascular endothelial damage after treatment; while the Chinese medicine composition group of Comparative Examples 1-3 has a weaker effect on improving mouse microvascular damage, indicating that the distribution ratio of each group in the Chinese medicine composition is not within the scope of the present invention, which will weaken the improvement effect of the Chinese medicine composition on microvascular endothelial damage.

[0073] Efficacy test 2:

[0074] Experimental samples: Chinese medicine compositions prepared in Examples 1-4 and Comparative Examples 1-3.

[0075] Experimental animal grouping: 6-week-old SPF-grade C57BL / 6 mice were purchased as experimental mice and randomly divided into normal group, model group, comparative example 1-3 Chinese medicine composition group, Example 1 Chinese medicine composition low-dose group, Example 1 Chinese medicine composition medium-dose group, Example 1 Chinese medicine composition high-dose group, Example 2-4 Chinese medicine composition group, positive drug group (folic acid), 6 mice in each group. The mice in the normal group were given ordinary drinking water, and the mice in the model group and drug group were given drinking water containing homocysteine ​​(1.8 g / L). The low-dose group of the Chinese medicine composition of Example 1 was gavaged with 5.3 g crude drug / kg daily from the beginning of modeling, the medium-dose group of the Chinese medicine composition of Example 1 was gavaged with 10.4 g crude drug / kg daily, the high-dose group of the Chinese medicine composition of Example 1 was gavaged with 20.9 g crude drug / kg daily, the Chinese medicine composition groups of Comparative Examples 1-3 and the Chinese medicine composition groups of Examples 2-4 were gavaged with 20.9 g crude drug / kg daily from the beginning of modeling, and the positive drug group was gavaged with 71 μg / kg of folic acid daily for 6 consecutive weeks. The normal group and the model group were administered with the same volume of physiological saline.

[0076] Six weeks after administration, the mice were anesthetized and the blood flow of the mice's feet was detected using a laser speckle blood flow imaging system. After the mice were anesthetized, they were placed in a suitable position to expose the part to be tested. The camera focus was adjusted under visible light to obtain a clear image, and then various parameters were adjusted according to different parts. After recording, the ROI area of ​​the part to be tested was selected using LSCI software (Shenzhen RWD), the average perfusion volume was calculated, and the physical image, grayscale image, pseudo-color image and video file were generated.

[0077] After anesthesia, the mice were killed, and the mouse heart tissue was collected for frozen sections, and the sections were stained using immunofluorescence staining. First, the slides were taken out and rinsed with 0.02% PBST three times, then the slides were placed in acetone and fixed at -20°C for 20 minutes, then the slides were taken out, 0.02% PBST was added and allowed to stand for 3 minutes, and repeated 3 times. The excess liquid around the tissue was wiped with lens paper, and a circle of appropriate size was drawn around the tissue with an immunohistochemistry pen, and the blocking solution was added for blocking at room temperature for 1 hour. The blocking solution was discarded, and the primary antibody (anti-CD31 / anti-VCAM-1 / anti-ZO-1) was added, and incubated at 4°C overnight. The primary antibody was discarded, and 0.02% PBST was added and allowed to stand for 5 minutes, and repeated three times. The PBST was dried, and the fluorescent secondary antibody was added, and incubated at room temperature in the dark for 2 hours. The secondary antibody was discarded, 0.02% PBST was added and allowed to stand for 5 min, and this process was repeated three times. Excess water around the tissue and traces of the immunohistochemical pen were wiped off, the slides were sealed in a dark place, and photographed under an immunofluorescence microscope.

[0078] Mice drinking drinking water containing homocysteine ​​(Hcy) will develop hyperhomocysteinemia. High homocysteine ​​can promote microcirculatory disorders. Figure 2 As shown, the results of laser speckle blood flow imaging showed that the blood flow in both feet of the normal group mice was abundant (the color was orange-red); while the blood flow in the forelimbs of the model group mice was significantly reduced (the color was green-blue). After the intervention of the Chinese medicine composition of Examples 1-4 and the positive drug, the blood flow in the forelimbs of the mice was significantly increased, while the Chinese medicine composition of the comparative example 1-3 group had no obvious effect, indicating that the Chinese medicine composition can improve the microcirculation disorder caused by hyperhomocysteinemia.

[0079] Tight junction protein ZO-1 is one of the important proteins that characterize vascular endothelial function. When the vascular endothelium is damaged, the expression of ZO-1 will decrease. Vascular cell adhesion molecule 1 (VCAM-1) is expressed at a low level on resting vascular endothelial cells. When the blood vessels are damaged, the expression of ICAM-1 will be significantly upregulated. Figure 3 As shown, when mice were treated with homocysteine ​​(Hcy), endothelial damage occurred in the mouse heart microvessels. Compared with the normal group, the expression of VCAM-1 in the heart microvascular endothelial of the mice in the model group was significantly increased, and the expression of ZO-1 was significantly reduced. After intervention with the Chinese medicine composition of Examples 1-4 and the positive drug, the vascular endothelial damage was significantly improved; while the improvement effect of the Chinese medicine composition group of Comparative Examples 1-3 on the microvascular damage of mice was weak, indicating that the distribution ratio of each group in the Chinese medicine composition is not within the scope of the present invention, which will weaken the improvement effect of the Chinese medicine composition on the microvascular endothelial damage.

[0080] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. Use of a Chinese medicine composition for improving microcirculatory disorders in the preparation of a medicament for treating hyperhomocysteinemia, characterized in that: The Chinese medicinal composition for improving microcirculation disorder is composed of the following components in parts by weight: 1-10 parts of Gastrodia elata, 1-5 parts of Panax notoginseng, 1-10 parts of lotus seed core, and 5-30 parts of corn silk.

2. The use according to claim 1, characterized in that The Chinese medicine composition for improving microcirculation disorder is composed of the following components in parts by weight: 9 parts of Gastrodia elata, 3 parts of Panax notoginseng, 9 parts of lotus seed core, and 30 parts of corn silk.

3. The use according to claim 1, characterized in that The preparation method of the Chinese medicine composition for improving microcirculation disorder comprises the following steps: (1) Weighing medicinal materials according to weight ratio, mixing, soaking in water, heating and steaming, filtering, and obtaining an extract A and a filter residue; (2) taking the filter residue, adding water and boiling it, and filtering it to obtain extract B; (3) The extract A and the extract B are mixed, allowed to stand, filtered, and concentrated to obtain the Chinese medicine composition for improving microcirculation disorders.

4. The use according to claim 3, characterized in that In the step (1), the amount of water added is 8-30 times the total weight of the medicinal material raw materials; the soaking time is 0.5-3h; and the steaming time is 30-45min.

5. The use according to claim 3, characterized in that In the step (2), the cooking time is 20 min-2 h.

6. The use according to claim 3, characterized in that In the step (3), the concentration is carried out by vacuum distillation.

7. The use according to claim 3, characterized in that In the step (3), the mixture is concentrated to a clear paste with a relative density of 1.0-1.

2.

8. The use according to claim 1, characterized in that The dosage forms of the medicine include tablets, capsules, syrups and mixtures.

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