A nucleic acid amplification detection module and nucleic acid amplification detection all-in-one machine

Abstract

The present application relates to a nucleic acid amplification detection module and a nucleic acid amplification detection all-in-one machine, the nucleic acid amplification detection module includes the fluorescence detection mechanism and temperature adjusting mechanism that are oppositely arranged along the first direction, carrier for carrying reaction sample can be pressed to the side of temperature adjusting mechanism facing fluorescence detection mechanism, fluorescence detection mechanism is used to detect reaction sample, temperature adjusting mechanism is used to heat and cool reaction sample.Fluorescence detection mechanism, carrier temperature adjusting mechanism are sequentially arranged along the first direction, so that the nucleic acid amplification detection module is compact, carrier can be pressed to temperature adjusting mechanism, so that the surface of carrier can be in contact with the surface of temperature adjusting mechanism, increase the contact area between carrier and temperature adjusting mechanism, improve the heat transfer efficiency and amplification efficiency.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of in vitro diagnosis, and in particular to a nucleic acid amplification detection module and a nucleic acid amplification detection all-in-one machine. BACKGROUND

[0002] In the prior art, PCR (Polymerase Chain Reaction) refers to a molecular biology experiment method for in vitro enzymatic synthesis of specific DNA fragments, which mainly consists of three steps of high-temperature denaturation, low-temperature annealing and suitable temperature extension repeated heat cycle. Before PCR amplification, the reaction sample needs to be placed in a carrier, wherein the reaction sample is composed of collected throat swabs or nasal swabs and reagents for PCR amplification. During PCR amplification, the reaction sample needs to be heated by a heating sheet and cooled by a cooler (22), so that the reaction sample cycles in the stages of high-temperature denaturation, low-temperature annealing and suitable temperature extension.

[0003] When the PCR amplification machine heats the sample, the sample needs to be placed in a carrier. The PCR amplification machine in the prior art is mostly used for heating tube-shaped carriers. The contact area between the carrier and the heating mechanism is small, and the liquid layer is thick, which leads to slow heating and cooling speed of the reaction sample, and further affects the amplification efficiency.

[0004] In addition, the PCR amplification machine in the prior art drives the heating mechanism to translate by a motor, so that the heating mechanism is pressed on the carrier to improve the heat transfer efficiency between the heating mechanism and the carrier. However, the motor is large in size and occupies a large space, so that the PCR amplification machine cannot be miniaturized. At the same time, the wire harness and pipeline connected with the heating mechanism need to have a certain excess length, so as not to be pulled off when the heating mechanism translates. Therefore, the excess wire harness and pipeline also need to occupy a certain space.

[0005] In addition, the fluorescence detection of the reaction sample often needs to be carried out in a relatively dark space to avoid the influence of light on the detection structure. In the prior art, a cover is installed on the shell. After the cover is opened, the motor drives the heating mechanism to move outside the shell for placing and taking the carrier. This makes the steps of placing and taking the carrier more, and the operation more complicated, which leads to low working efficiency. Alternatively, the heating mechanism is similar to a drawer and is connected to the shell in a pullable manner. The carrier can be placed after the heating mechanism is pulled out, and the carrier is in a relatively dark environment after the heating mechanism is pushed into the shell. At the same time, after the carrier is located in the shell, an additional pressing mechanism is needed to press the carrier on the heating mechanism. Therefore, structures cooperating with the heating mechanism for pulling and the pressing mechanism need to be set, which increases the complexity of the PCR amplification machine and leads to the difficulty of miniaturization of the PCR amplification machine. SUMMARY

[0006] An object of the present application is to provide a nucleic acid amplification detection module to at least solve one of the above technical problems.

[0007] To achieve the above object, the present application provides a nucleic acid amplification detection module, comprising a fluorescence detection mechanism and a temperature adjusting mechanism oppositely arranged along a first direction, a carrier for carrying a reaction sample being capable of being pressed against one side of the temperature adjusting mechanism facing the fluorescence detection mechanism, the fluorescence detection mechanism being used for detecting the reaction sample, and the temperature adjusting mechanism being used for heating and cooling the reaction sample.

[0008] Optionally, the nucleic acid amplification detection module further comprises a pressing device, the pressing device being capable of being in a pressing state of pressing the carrier against the temperature adjusting mechanism or a loosening state of loosening the carrier.

[0009] Optionally, the fluorescence detection mechanism or the temperature adjusting mechanism is arranged in the pressing device.

[0010] Optionally, the pressing device comprises a linear mechanism and a pressing mechanism, the linear mechanism being used for driving the pressing mechanism to translate so as to press or loosen the carrier.

[0011] Optionally, the linear mechanism is a cam mechanism, or a gear mechanism, or a crank slider mechanism.

[0012] Optionally, the nucleic acid amplification detection module further comprises a housing, the pressing device, the fluorescence detection mechanism and the temperature adjusting mechanism all being arranged in the housing, the housing being provided with an opening, and the carrier entering or exiting the housing through the opening.

[0013] Optionally, the cam mechanism comprises a cam and a first guide rod, the cam being rotationally connected to the housing, the first guide rod being connected to the housing and extending along a first direction, the pressing mechanism being a first pressing mechanism, the first pressing mechanism being slidingly connected to the first guide rod, and the cam abutting against the first pressing mechanism.

[0014] Optionally, the cam has a circumferential surface abutting against the first pressing mechanism, and the circumferential surface gradually moves away from a rotation center of the cam from one end to another end in a circumferential direction.

[0015] Optionally, the cam mechanism further comprises a first locking piece, the first locking piece being arranged on the first guide rod and abutting against the first pressing mechanism, so that the first pressing mechanism always abuts against the cam.

[0016] Optionally, one end of the first guide rod is connected with a first flange, and the first locking piece comprises a spring, the spring being sleeved on the first guide rod, one end of the spring abutting against the first flange, and the other end of the spring abutting against the first pressing mechanism.

[0017] Optionally, the cam is arranged on one side of the fluorescence detection mechanism or the temperature adjusting mechanism along a second direction, the first direction being perpendicular to the second direction.

[0018] Optionally, two cams are arranged along the second direction, the two cams being connected by a first connecting shaft, and the two cams respectively abutting against the first pressing mechanism.

[0019] Optionally, the fluorescence detection mechanism or the temperature adjusting mechanism is located between the two cams.

[0020] Optionally, the first pressing mechanism comprises a pressing frame and a first pressing plate, the pressing frame being slidingly connected with the first guide rod, and the first pressing plate being elastically connected with the pressing frame.

[0021] Optionally, the first pressing mechanism further comprises a pressing wheel for abutting against the cam, the pressing wheel being rotationally connected with the pressing frame.

[0022] Optionally, the pressing frame is an annular frame.

[0023] Optionally, a first buffer is arranged between the pressing frame and the first pressing plate.

[0024] Optionally, the first pressing mechanism further comprises a second guide rod, the second guide rod being connected with the pressing frame and extending along the first direction, and the first pressing plate being slidingly connected with the second guide rod.

[0025] Optionally, the first buffer is a spring, the spring being sleeved outside the second guide rod and located between the pressing frame and the first pressing plate.

[0026] Optionally, the fluorescence detection mechanism or the temperature adjusting mechanism is arranged in the pressing frame, a first avoiding hole is formed in the first pressing plate, and the fluorescence detection mechanism detects the reaction sample through the first avoiding hole.

[0027] Optionally, the gear mechanism comprises a driving gear, a driven gear meshing with the driving gear, and a transmission assembly, the driven gear being provided with an arc-shaped guide hole, the pressing mechanism being a second pressing mechanism, the arc-shaped guide hole gradually moving away from the center of the driven gear from one end to the other end, the transmission assembly being slidingly connected with the housing along the first direction, one end of the transmission assembly being slidingly arranged in the arc-shaped guide hole, and the other end of the transmission assembly being connected with the second pressing mechanism to drive the second pressing mechanism to translate.

[0028] Optionally, the gear mechanism further comprises a second locking member, the second locking member being arranged on the transmission assembly and abutting against the second pressing mechanism, so that the transmission assembly always abuts against one side of the inner wall of the arc-shaped guide hole.

[0029] Optionally, the gear mechanism further comprises a fixed guide, the fixed guide is fixedly connected to the shell, and the transmission assembly is slidably connected to the fixed guide in the first direction.

[0030] Optionally, the second locking member comprises a spring, the spring is sleeved on the transmission assembly and located between the fixed guide and the second pressing mechanism.

[0031] Optionally, the second pressing mechanism comprises a second pressing plate, the transmission assembly is slidably arranged through the second pressing plate, the second pressing plate is used for pressing the carrier, and a second avoiding hole is formed in the second pressing plate, and the fluorescence detection mechanism detects the reaction sample through the second avoiding hole.

[0032] Optionally, a second buffer is arranged between the gear mechanism and the second pressing mechanism.

[0033] Optionally, the other end of the transmission assembly is connected with a second flange, the second buffer is a spring, the spring is sleeved outside the transmission assembly and located between the second flange and the second pressing plate.

[0034] Optionally, the second pressing mechanism further comprises a pre-pressing block for pre-pressing the second buffer, the pre-pressing block is connected to the second pressing plate, the pre-pressing block is provided with an accommodating groove, the second buffer and the second flange are arranged in the accommodating groove, and the second flange is capable of sliding relative to the pre-pressing block.

[0035] Optionally, the driven gear and the transmission assembly are arranged on one side of the fluorescence detection mechanism or the temperature adjusting mechanism in the second direction.

[0036] Optionally, two groups of the transmission assemblies are arranged in the second direction, the two groups of the transmission assemblies are connected through a second connecting shaft, the second connecting shaft is slidably arranged in the arc-shaped guide hole, and the fluorescence detection mechanism or the temperature adjusting mechanism is located between the two groups of the transmission assemblies.

[0037] Optionally, the crank slider mechanism comprises a connecting rod, a slider and a third guide rod, the pressing mechanism is a third pressing mechanism, the third guide rod is connected to the shell and extends in the first direction, the slider is sleeved outside the third guide rod and slides along the third guide rod, one end of the connecting rod is rotationally connected to the slider and drives the slider to slide along the third guide rod, and the slider is used for driving the third pressing mechanism to press the carrier.

[0038] Optionally, the crank slider mechanism further comprises a third locking member, the third locking member is arranged on the third guide rod and abuts against the third pressing mechanism, the third locking member drives the third pressing mechanism to move to the side where the slider is located.

[0039] Optionally, an end of the third guide rod is connected with a third flange, the third pressing mechanism comprises a pressing rod, the pressing rod is slidingly connected with the third guide rod, the third locking member is a spring, the spring is sleeved on the third guide rod and abuts against the pressing rod and the third flange.

[0040] Optionally, the third pressing mechanism further comprises a third pressing plate, the third pressing plate is provided with a third avoiding hole, the fluorescent detection mechanism detects the reaction sample through the third avoiding hole.

[0041] Optionally, a third buffer member is arranged between the slider and the third pressing mechanism.

[0042] Optionally, the slider and the third guide rod are arranged on one side of the fluorescent detection mechanism or the temperature adjusting mechanism in the second direction.

[0043] Optionally, two groups of the slider and the third guide rod are arranged in the second direction, the two groups of the slider are connected through a third connecting shaft, the connecting rod is connected with the third connecting shaft, and the fluorescent detection mechanism or the temperature adjusting mechanism is located between the two groups of the third guide rod.

[0044] Optionally, the nucleic acid amplification detection module further comprises a driving member, the driving member is connected with the pressing device and drives the pressing device to switch between the pressing state and the loosening state.

[0045] Optionally, the driving member is a cover body, the cover body can be in an opening state of opening the opening or a closing state of closing the opening.

[0046] Optionally, the cover body is switched from the opening state to the closing state, which can drive the pressing device to switch from the loosening state to the pressing state; the cover body is switched from the closing state to the opening state, which can drive the pressing device to switch from the pressing state to the loosening state.

[0047] Optionally, the pressing device further comprises a pressing detection mechanism, the pressing detection mechanism is used for detecting whether the pressing mechanism presses the carrier.

[0048] Optionally, the pressing detection mechanism comprises a photoelectric switch and a blocking piece, one of the photoelectric switch and the blocking piece is connected to the pressing mechanism, and the other is fixedly connected to the shell, the pressing mechanism is in translation, and the blocking piece can enter and exit between the emitting part and the receiving part of the photoelectric switch.

[0049] Optionally, a carrier for inserting a flat structure is arranged between the fluorescence detection mechanism and the temperature adjusting mechanism.

[0050] Optionally, the temperature adjusting mechanism comprises a heating sheet for heating the carrier and a cooler for cooling the heating sheet, and the heating sheet is arranged on one side of the cooler along a first direction.

[0051] Optionally, the nucleic acid amplification detection module further comprises an insertion block arranged on a side of the temperature adjusting mechanism facing the fluorescence detection mechanism, and the insertion block is provided with an insertion slot for inserting the carrier.

[0052] Optionally, the nucleic acid amplification detection module further comprises an in-place detection mechanism for detecting whether the carrier is inserted into the insertion slot.

[0053] Optionally, the in-place detection mechanism comprises a travel switch connected to the insertion block, and the carrier inserted into the insertion slot can press the travel switch.

[0054] Optionally, the in-place detection mechanism further comprises a pressing sheet, the pressing sheet is rotationally connected to the insertion block, and the carrier inserted into the insertion slot can press the pressing sheet, so that the pressing sheet presses the travel switch.

[0055] Optionally, the nucleic acid amplification detection module further comprises a to-position detection mechanism for detecting whether the carrier is inserted to a preset position.

[0056] Optionally, the to-position detection mechanism comprises a to-position protrusion elastically connected to the insertion block, and a to-position notch is arranged on the carrier, and the to-position protrusion can enter and exit the to-position notch.

[0057] Optionally, the nucleic acid amplification detection module further comprises a squeezing mechanism for squeezing a squeezing cavity on the carrier, and the squeezing mechanism is connected to the temperature adjusting mechanism.

[0058] Optionally, the squeezing mechanism comprises a squeezing fixed block, a pressing head and a squeezing elastic piece, the squeezing fixed block is connected to the temperature adjusting mechanism and is provided with a stepped hole, the pressing head is partially arranged in the stepped hole and can abut against a stepped surface of the stepped hole, and the other part of the pressing head protrudes out of the stepped hole, and the squeezing elastic piece is used for adjusting the length of the pressing head protruding out of the stepped hole.

[0059] Optionally, the cooler comprises a cooling body and a base, the cooling body is capable of circulating cooling liquid to cool the heating sheet, and the base is provided with a mounting groove, and the cooling body is mounted in the mounting groove.

[0060] Another object of the present application is to provide a nucleic acid amplification and detection integrated machine to at least solve one of the above technical problems.

[0061] To achieve the above object, the second aspect of the present application adopts the following technical solution:

[0062] A nucleic acid amplification and detection integrated machine comprises the nucleic acid amplification and detection module.

[0063] Optionally, the nucleic acid amplification and detection integrated machine comprises one or more groups of the nucleic acid amplification and detection module.

[0064] Optionally, the nucleic acid amplification and detection integrated machine further comprises a water chiller, and the water chiller is used to provide cooling medium for the temperature adjusting mechanism.

[0065] Optionally, the nucleic acid amplification and detection integrated machine further comprises a control module, and the control module is electrically connected with the water chiller and the nucleic acid amplification and detection module.

[0066] As can be seen from the above, the technical solution provided by the present application, the nucleic acid amplification and detection module comprises a fluorescence detection mechanism and a temperature adjusting mechanism which are oppositely and spacedly arranged along a first direction, and the carrier for carrying the reaction sample can be pressed against a side of the temperature adjusting mechanism facing the fluorescence detection mechanism, i.e., the fluorescence detection mechanism, the carrier and the temperature adjusting mechanism are sequentially arranged along the first direction, so that the nucleic acid amplification and detection module is compact in structure. The temperature adjusting mechanism is used to heat and cool the reaction sample to realize pcr amplification of the reaction sample, and the carrier can be pressed against the temperature adjusting mechanism, so that the surface of the carrier can be in contact with the surface of the temperature adjusting mechanism, the contact area between the carrier and the temperature adjusting mechanism is increased, and the heat transfer efficiency and the amplification efficiency are improved. The fluorescence detection mechanism is used to detect the amplified reaction sample, and since the carrier is located on the side of the temperature adjusting mechanism close to the fluorescence detection mechanism, after the reaction sample is amplified, the fluorescence detection mechanism can directly detect the reaction sample without transferring the carrier, so that the detection efficiency is improved.

[0067] The carrier is pressed against the temperature adjusting mechanism by the state change of the pressing device, and the temperature adjusting mechanism does not need to move to press the carrier any more, and the wire harness and pipeline connected with the temperature adjusting mechanism do not need to be provided with the length required for the movement of the temperature adjusting mechanism, so that the space occupied by the excess wire harness and pipeline can be saved, and the nucleic acid amplification and detection module can be miniaturized.

[0068] When the pressing device is in the pressing state, the carrier can be in contact with the temperature adjusting mechanism, so as to ensure the heat conduction efficiency. In addition, after the pressing mechanism presses the carrier, the extruding mechanism extrudes the extruding cavity, the air in the extruding cavity is compressed, and then the reaction sample in the containing cavity is extruded, so that the deformable wall is tightly attached to the temperature adjusting mechanism. It can be understood that the temperature adjusting mechanism can stop the deformable wall from protruding outwardly. Therefore, when the reaction sample extrudes the deformable wall, the deformable wall is still in a planar state and is tightly attached to the temperature adjusting mechanism, so as to ensure the contact area between the deformable wall and the temperature adjusting mechanism, and ensure that the reaction sample and the temperature adjusting mechanism have high heat conduction efficiency.

[0069] The carrier enters between the fluorescence detection mechanism and the temperature adjusting mechanism by insertion, and the fluorescence detection mechanism and the temperature adjusting mechanism can put the carrier in place or take out the carrier without moving, so that the fluorescence detection mechanism and the temperature adjusting mechanism do not need more activity space, which improves the compactness of the nucleic acid amplification fluorescence detection mechanism. At the same time, the line connected with the fluorescence detection mechanism and the wire harness and pipeline connected with the temperature adjusting mechanism do not need to be provided with excess length, which saves space. In addition, since the fluorescence detection mechanism and the temperature adjusting mechanism do not need to move, the nucleic acid amplification detection module does not need to be configured with a structure cooperating with the movement of the fluorescence detection mechanism and the temperature adjusting mechanism, which simplifies the structure of the nucleic acid amplification detection module, thereby facilitating the miniaturization of the nucleic acid amplification detection module.

[0070] The cover body is linked with the pressing device, and the cover body can drive the pressing device and close and open the opening. At the same time, the pressing device does not need to be driven by a motor or the like, which saves the space of the nucleic acid amplification detection module and helps to realize the miniaturization of the nucleic acid amplification detection module.

[0071] The cover body is switched from the open state to the closed state, which can drive the pressing device to switch from the loosened state to the pressed state. The cover body is switched from the closed state to the open state, which can drive the pressing device to switch from the pressed state to the loosened state. That is, when the cover body is opened, the cover body can drive the pressing device to loosen the carrier, so that after the opening is opened, the operator can take out the carrier. After the carrier is inserted, when the cover body is closed, the cover body can drive the pressing device to press the carrier, so that the carrier is tightly attached to the temperature adjusting mechanism. The closing of the cover body is synchronized with the pressing of the carrier, and the opening of the cover body is synchronized with the loosening of the carrier, so that the steps of taking and placing the carrier are simple and the work efficiency is high.

[0072] The fluorescence detection mechanism is arranged in the pressing device, so that the pressing device can fully utilize the free space on at least one side of the fluorescence device along the second direction, so that the nucleic acid amplification detection module is compact in structure and the volume of the nucleic acid amplification detection module can be reduced. BRIEF DESCRIPTION OF DRAWINGS

[0073] Figure 1is a top view of the fluorescence detection mechanism, carrier and temperature adjusting mechanism provided by the embodiment of the present application;

[0074] Figure 2a is a structural schematic view of the carrier provided by the embodiment of the present application;

[0075] Figure 2b is a structural schematic view of another carrier provided by the embodiment of the present application;

[0076] Figure 3 is a structural schematic view of the temperature adjusting mechanism, in-place detection mechanism, to-place detection mechanism and plug block provided by the embodiment of the present application, which carries the carrier;

[0077] Figure 4 is a structural schematic view of the temperature adjusting mechanism, in-place detection mechanism, to-place detection mechanism and plug block provided by the embodiment of the present application, which does not carry the carrier; Figure 3

[0078] Figure 5 is a structural schematic view of the plug block provided by the embodiment of the present application;

[0079] Figure 6 is a sectional view of the to-place detection mechanism provided by the embodiment of the present application;

[0080] Figure 7 is a structural schematic view of the in-place detection mechanism provided by the embodiment of the present application;

[0081] Figure 8a is a sectional view of the partial nucleic acid amplification detection module provided by the embodiment of the present application, which carries the carrier;

[0082] Figure 8b is a sectional view of the partial nucleic acid amplification detection module provided by the embodiment of the present application, which does not carry the carrier; Figure 8a is an enlarged view of A in the above figure;

[0083] Figure 9 is an exploded view of the temperature adjusting mechanism and plug block provided by the embodiment of the present application;

[0084] Figure 10 is an exploded view of the temperature adjusting mechanism and plug block provided by the embodiment of the present application, from another perspective;

[0085] Figure 11 is a structural schematic view of the partial cooling main body provided by the embodiment of the present application;

[0086] Figure 12 is a structural schematic view of the nucleic acid amplification detection module provided by the embodiment of the present application, with the cover covering the opening;

[0087] Figure 13 is a structural schematic view of the nucleic acid amplification detection module provided by the embodiment of the present application, with the cover opening the opening;

[0088] Figure 14 ​is a structural schematic view of the first nucleic acid amplification detection module (cover body opening) removing part of the shell provided by the embodiment of the present application;

[0089] Figure 15 is a structural schematic view of the first nucleic acid amplification detection module (cover body covering the opening) removing part of the shell provided by the embodiment of the present application;

[0090] Figure 16 is a structural schematic view of the first nucleic acid amplification detection module hiding part of the shell provided by the embodiment of the present application;

[0091] Figure 17a is an exploded view of part of the first nucleic acid amplification detection module (cover body covering the opening) provided by the embodiment of the present application;

[0092] Figure 17b is an exploded view of part of the first nucleic acid amplification detection module (cover body covering the opening) provided by the embodiment of the present application;

[0093] Figure 18 is another schematic view of part of the first nucleic acid amplification detection module (cover body covering the opening) provided by the embodiment of the present application;

[0094] Figure 19a is another sectional view of part of the first nucleic acid amplification detection module provided by the embodiment of the present application;

[0095] Figure 19b is another exploded view of part of the first nucleic acid amplification detection module provided by the embodiment of the present application;

[0096] Figure 19c is an exploded view of the first pressing mechanism provided by the embodiment of the present application;

[0097] Figure 20 is a sectional view of part of the first nucleic acid amplification detection module at another position provided by the embodiment of the present application;

[0098] Figure 21a is a structural schematic view of the pressing frame provided by the embodiment of the present application;

[0099] Figure 21b is a structural schematic view of the fluorescence detection mechanism arranged in the pressing frame provided by the embodiment of the present application;

[0100] Figure 22 is a schematic view of part of the first nucleic acid amplification detection module (cover body covering the opening, fluorescence detection mechanism not removed) from another perspective provided by the embodiment of the present application;

[0101] Figure 23 is a structural schematic view of the first nucleic acid amplification detection module removing part of the shell provided by the embodiment of the present application;

[0102] Figure 24a is a partial structure diagram of a first nucleic acid amplification detection module (remove the cover and part of the shell) provided by another perspective of the embodiment of the application;

[0103] Figure 24b is a schematic view of the temperature adjusting mechanism provided in the compression device;

[0104] Figure 25 is a partial structure diagram of a second nucleic acid amplification detection module (cover closes the opening) provided by the embodiment of the application;

[0105] Figure 26 is a partial structure diagram of a second nucleic acid amplification detection module (cover opens the opening) provided by the embodiment of the application;

[0106] Figure 27 is an exploded view of a partial structure of a second nucleic acid amplification detection module provided by the embodiment of the application;

[0107] Figure 28 is a partial structure diagram of a second nucleic acid amplification detection module provided by another perspective of the embodiment of the application;

[0108] Figure 29a is an exploded view of a transmission assembly provided by the embodiment of the application;

[0109] Figure 29b is a schematic view of the fluorescence detection mechanism provided in the compression device;

[0110] Figure 30 is a partial structure diagram of a third nucleic acid amplification detection module (cover closes the opening) provided by the embodiment of the application;

[0111] Figure 31 is a partial structure diagram of a third nucleic acid amplification detection module (cover opens the opening) provided by the embodiment of the application;

[0112] Figure 32 is a partial structure diagram of a third nucleic acid amplification detection module provided by the embodiment of the application;

[0113] Figure 33 is a structure diagram of a nucleic acid amplification detection all-in-one machine provided by the embodiment of the application.

[0114] In the figure:

[0115] 1, fluorescence detection mechanism;

[0116] 2, temperature adjusting mechanism;

[0117] 22, cooler;

[0118] 221, cooling body; 2211, cooling fin; 2212, cooling shell; 2213, sealing ring; 2214, sealing cover; 2215, blind hole; 2216, medium inlet; 2217, medium outlet;

[0119] 222, heat-conducting temperature measurement plate; 2221, temperature measurement groove;

[0120] 223, base; 2231, mounting groove; 224, temperature sensor;

[0121] 23, heating sheet;

[0122] 3, shell; 4, cover;

[0123] 5, pressing device;

[0124] 51, cam mechanism;

[0125] 511, cam; 5111, U-shaped groove; 5112, peripheral surface; 512, first guide rod; 513, first locking piece; 514, first flange; 515, transmission part; 5151, transmission body; 5152, pulley; 516, first connecting shaft; 517, first fixed plate; 518, mounting block; 519, second fixed plate;

[0126] 52, gear mechanism;

[0127] 521, driving gear; 5211, arc-shaped guide hole; 522, driven gear; 523, transmission assembly; 5231, transmission piece; 5232, fourth guide rod; 5233, shaft hole; 524, second locking piece; 525, second flange; 526, second connecting shaft; 527, fixed guide piece; 528, second buffer piece; 529, gear fixing piece;

[0128] 53, crank slider mechanism;

[0129] 531, connecting rod; 532, slider; 533, third guide rod; 534, third locking piece; 535, third flange; 536, third buffer piece;

[0130] 54, first pressing mechanism;

[0131] 541, pressing frame; 5411, guide stepped hole; 542, first pressing plate; 5421, first avoiding hole; 5422, sliding through hole; 543, pressing wheel; 544, first buffer piece; 545, second guide rod;

[0132] 55, second pressing mechanism;

[0133] 551, second pressing plate; 5511, second avoiding hole; 552, pre-pressing block; 553, accommodating groove;

[0134] 56, third pressing mechanism;

[0135] 561, pressing rod; 5611, first rod; 5612, second rod; 562, third pressing plate; 5621, third avoiding hole;

[0136] 57, pressing detection mechanism; 571, photoelectric switch; 572, blocking piece;

[0137] 6, extruding mechanism; 61, pressing head; 611, abutting convex ring; 62, extruding elastic piece;

[0138] 7, in-position detection mechanism; 71, travel switch; 72, pressing piece; 73, rotating shaft; 74, mounting piece;

[0139] 8, to-position detection mechanism; 81, to-position protrusion; 82, to-position fixing block; 83, to-position elastic piece; 84, adjusting rod; 841, adjusting blind hole; 842, blocking ring;

[0140] 9, insertion block; 91, insertion groove; 911, entrance end; 92, stepped hole;

[0141] 10, nucleic acid amplification detection module; 20, water-cooling machine; 30, control module;

[0142] 100, carrier; 101, extruding cavity; 102, to-position notch; 103, accommodating cavity; 104, sealing piece; 105, sealing cavity. DETAILED DESCRIPTION

[0143] The technical solutions of the present application will be further described below in combination with the drawings and through specific embodiments. It can be understood that the specific embodiments described herein are only used to explain the present application, but not to limit the present application. In addition, it should be noted that, for the convenience of description, only the parts related to the present application are shown in the drawings, but not all.

[0144] In the present application, some orientation words are defined. Without making the opposite statement, the orientation words used such as "upper", "lower", "left", "right", "inner", "outer" are used for the convenience of understanding, and thus do not constitute the limitation to the protection scope of the present application.

[0145] In the present application, unless otherwise explicitly specified and limited, "on" or "under" of a first feature to a second feature can include that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, "on", "above" and "over" of a first feature to a second feature includes that the first feature is directly above and obliquely above the second feature, or only indicates that the first feature is higher in horizontal height than the second feature. "Under", "below" and "underneath" of a first feature to a second feature includes that the first feature is directly below and obliquely below the second feature, or only indicates that the first feature is lower in horizontal height than the second feature.

[0146] In the description of the present application, unless otherwise explicitly specified and limited, the terms "connected", "connected", "fixed" should be understood broadly, for example, can be fixedly connected, or can be detachably connected, or integrated; can be mechanically connected, or electrically connected; can be directly connected, or indirectly connected through an intermediate medium, can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances. Embodiment one

[0147] The present embodiment provides a nucleic acid amplification detection module 10 for pcr amplification and fluorescence detection of the reaction sample after pcr amplification, so as to improve the amplification efficiency, reduce the volume of the nucleic acid amplification detection module 10, and realize the miniaturization of the nucleic acid amplification detection module 10 and the nucleic acid amplification detection all-in-one machine with the nucleic acid amplification detection module 10.

[0148] As shown in Figure 1 The nucleic acid amplification detection module 10 includes a fluorescence detection mechanism 1 and a temperature adjusting mechanism 2 arranged in a first direction X, and a carrier 100 for carrying a reaction sample can be pressed against the side of the temperature adjusting mechanism 2 facing the fluorescence detection mechanism 1, that is, the fluorescence detection mechanism 1, the carrier 100 and the temperature adjusting mechanism 2 are arranged in the first direction X in turn, so that the nucleic acid amplification detection module 10 is compact in structure. The temperature adjusting mechanism 2 is used for heating and cooling the reaction sample to realize pcr amplification of the reaction sample, and the carrier 100 can be pressed against the temperature adjusting mechanism 2, so that the surface of the carrier 100 can be in contact with the surface of the temperature adjusting mechanism 2, the contact area between the carrier 100 and the temperature adjusting mechanism 2 is increased, and the heat transfer efficiency and the amplification efficiency are improved. The fluorescence detection mechanism 1 is used for detecting the amplified reaction sample. Since the carrier 100 is located on the side of the temperature adjusting mechanism 2 close to the fluorescence detection mechanism 1, after the reaction sample is amplified, the fluorescence detection mechanism 1 can directly detect the reaction sample without transferring the carrier 100, so as to improve the detection efficiency.

[0149] Exemplarily, in the embodiment, the first direction X and the second direction Y are both horizontal directions, and the vertical direction Z, the first direction X and the second direction Y are perpendicular to each other. Of course, in other optional embodiments, the first direction X can also be the vertical direction Z or other directions according to the placement mode of the nucleic acid amplification detection module 10.

[0150] As shown in Figure 1 、 Figure 2a and Figure 2b , the carrier 100 in a flat structure is inserted between the fluorescence detection mechanism 1 and the temperature adjusting mechanism 2. It can be understood that the flat structure can refer to that the size of the carrier 100 in the thickness direction is much smaller than the size of the direction perpendicular to the thickness direction. Exemplarily, the ratio of the size of the direction perpendicular to the thickness direction to the size of the thickness direction is greater than 5:1, such as 50:1~100:1, exemplarily, the ratio is 90:1. Exemplarily, the carrier 100 is a cuboid, and the ratio of the length to the thickness of the cuboid can be greater than 5:1, such as 90:1. Exemplarily, the size of the carrier 100 in the thickness direction can be 0.3-1.0mm, and the width and length of the carrier 100 are about 10mm and 20mm respectively. Of course, the cross section of the carrier 100 can be cylindrical, polygonal or elliptical, etc.

[0151] The carrier 100 is in a flat structure, which can make the thickness of the reaction sample in the carrier 100 very thin, and the distance from the center of the reaction sample to the surface of the liquid is very small. When the carrier 100 is heated or cooled by the temperature adjusting mechanism 2, the temperature of the reaction sample can reach uniformity in a very short time, the heat transfer efficiency is high, and the heating and cooling speed of the reaction sample and the detection efficiency are greatly improved. The inner diameter of the PCR tube is relatively large compared with the flat structure carrier 100, the distance from the center of the reaction sample to the surface of the liquid is very large, and the temperature of the reaction sample needs a long time to reach uniformity. The heating and cooling speed of the reaction sample is low, and the detection efficiency is low.

[0152] In addition, the carrier 100 enters the fluorescence detection mechanism 1 and the temperature adjusting mechanism 2 by the way of insertion, and the fluorescence detection mechanism 1 and the temperature adjusting mechanism 2 can put the carrier 100 in place or take out the carrier 100 without moving, so that the fluorescence detection mechanism 1 and the temperature adjusting mechanism 2 do not need more moving space, which improves the compactness of the nucleic acid amplification fluorescence detection mechanism 1. At the same time, the line connected with the fluorescence detection mechanism 1 and the wire harness and pipeline connected with the temperature adjusting mechanism 2 do not need to be set with excess length, which saves space. In addition, since the fluorescence detection mechanism 1 and the temperature adjusting mechanism 2 do not need to move, the nucleic acid amplification detection module 10 does not need to be configured with a structure cooperating with the movement of the fluorescence detection mechanism 1 or the temperature adjusting mechanism 2, which simplifies the structure of the nucleic acid amplification detection module 10, thereby facilitating the miniaturization of the nucleic acid amplification detection module 10.

[0153] As shown in Figure 2a , the carrier 100 comprises a containing cavity 103 for containing a reaction sample and a compression cavity 101 in communication with the containing cavity 103, the containing cavity 103 and the compression cavity 101 are in communication through a flow channel, the cavity wall corresponding to the compression cavity 101 and the containing cavity 103 is a deformable wall (not shown in the figure), the compression cavity 101 is compressed, the gas in the compression cavity 101 is compressed, and in turn the reaction sample in the containing cavity 103 can be compressed, so that the corresponding deformable wall at the containing cavity 103 protrudes outward, so that the deformable wall is better attached to the temperature adjusting mechanism 2. Alternatively, the deformable wall can be a metal film such as an aluminum film. The side wall of the carrier 100 can also be provided with a to-position notch 102.

[0154] As shown in Figure 2b , the carrier 100 can also not include the compression cavity 101, but include a sealing member 104, the carrier 100 is provided with a sealing cavity 105, the sample containing cavity 103 is in communication with the sealing cavity 105, at least part of the sealing member 104 can be inserted into the sealing cavity 105 to at least compress the gas in the sealing cavity 105, and the deformable wall protrudes outward, so that the deformable wall is in close contact with the temperature adjusting mechanism 2.

[0155] As shown in Figures 3-5 , alternatively, the nucleic acid amplification detection module 10 can also include an insertion block 9 arranged on the side of the temperature adjusting mechanism 2 facing the fluorescence detection mechanism 1, the insertion block 9 is provided with an insertion slot 91 for inserting the carrier 100. The insertion block 9 can be connected to the temperature adjusting mechanism 2 by screws or other connecting members to fix the insertion block 9. The carrier 100 is inserted between the fluorescence detection mechanism 1 and the temperature adjusting mechanism 2 through the insertion slot 91, which can provide general positioning and guiding for the insertion of the carrier 100. The upper end of the insertion slot 91 has an entrance end 911, and the carrier 100 is inserted into the insertion slot 91 from the entrance end 911 in the vertical direction Z. The insertion slot 91 penetrates the insertion block 9 in the first direction X, so that one side of the carrier 100 in the first direction X abuts against the temperature adjusting mechanism 2, and the fluorescence detection mechanism 1 detects the reaction sample through the other side of the carrier 100 in the first direction X.

[0156] When inserting the carrier 100, it is not easy for the operator to observe whether the carrier 100 is inserted in place, in order to facilitate the operator to perceive whether the carrier 100 is inserted in place, as shown in Figure 3 and Figure 4 , alternatively, the nucleic acid amplification detection module 10 can also include a to-position detection mechanism 8 for detecting whether the carrier 100 is inserted into a preset position.

[0157] Specifically, the in-position detection mechanism 8 comprises an in-position protrusion 81 elastically connected with the insertion block 9, an in-position notch 102 formed on the carrier 100, and the in-position protrusion 81 capable of entering and exiting the in-position notch 102. Since the in-position protrusion 81 is elastically connected with the insertion block 9, during the insertion of the carrier 100, when the position on the sidewall of the carrier 100 where the in-position notch 102 is not formed is opposite to the in-position protrusion 81, the carrier 100 can extrude the in-position protrusion 81 to make the in-position protrusion 81 avoid the carrier 100, and when the in-position notch 102 is opposite to the in-position protrusion 81, the in-position protrusion 81 enters into the in-position notch 102, and the carrier 100 is inserted into the preset position. Since the in-position protrusion 81 is elastically connected with the insertion block 9, during the insertion of the carrier 100, the operator can perceive the in-position protrusion 81 entering into the in-position notch 102 to determine that the carrier 100 is inserted into the preset position.

[0158] As shown in Figure 6 , the in-position detection mechanism 8 can further comprise an in-position fixing block 82 and an in-position elastic member 83. The in-position fixing block 82 is fixedly connected with the insertion block 9 (in combination with Figure 4 ), and the in-position fixing block 82 is formed with a mounting hole, and the in-position protrusion 81 is at least partially located in the mounting hole and is limited to avoid falling from the in-position fixing block 82. The in-position elastic member 83 is arranged in the mounting hole, and the in-position protrusion 81 abuts against the in-position elastic member 83 to enter and exit the in-position notch 102. Optionally, the in-position elastic member 83 is a spring.

[0159] Further, in order to facilitate the adjustment of the position of the in-position protrusion 81 in the natural state, optionally, the in-position detection mechanism 8 can further comprise an adjusting rod 84, and the adjusting rod 84 is screw-connected in the mounting hole. Specifically, the adjusting rod 84 is formed with external threads, and the mounting hole is arranged with internal threads screw-connected with the external threads. The adjusting rod 84 is formed with an adjusting blind hole 841, and the in-position protrusion 81 is partially arranged in the adjusting blind hole 841 and partially protrudes out of the adjusting blind hole 841, so that the in-position protrusion 81 protruding out of the adjusting blind hole 841 enters into the in-position notch 102.

[0160] The in-position elastic member 83 is arranged in the adjusting blind hole 841, one end of the in-position elastic member 83 abuts against the in-position protrusion 81, and the other end of the in-position elastic member 83 abuts against the bottom wall of the adjusting blind hole 841. The length of the adjusting rod 84 screwing into the mounting hole can be adjusted, so that the position of the in-position protrusion 81 can be adjusted. Optionally, the in-position protrusion 81 is spherical, and the diameter of the spherical in-position protrusion 81 is smaller than the diameter of the adjusting blind hole 841, so as to put the in-position protrusion 81 into the adjusting blind hole 841. In order to avoid the in-position protrusion 81 from being separated from the adjusting blind hole 841, the end of the hole wall of the adjusting blind hole 841 can be inwardly bent to form a stop ring 842 stopping the in-position protrusion 81.

[0161] Continuing as Figure 3 and Figure 4As shown, the nucleic acid amplification detection module 10 may also include an in-situ detection mechanism 7, which is used to detect whether a carrier 100 is inserted in the slot 91, thereby preventing the nucleic acid amplification detection module 10 from malfunctioning when unloaded.

[0162] like Figure 7 As shown, and in combination Figure 4 The in-situ detection mechanism 7 includes a limit switch 71 connected to the insert block 9. The carrier 100 can press the limit switch 71 when inserted into the slot 91. The limit switch 71 can be electrically connected to the control module 30. After the carrier 100 presses the limit switch 71, the limit switch 71 transmits a signal to the control module 30, thereby detecting that the carrier 100 is placed inside the nucleic acid amplification detection module 10.

[0163] To facilitate the carrier 100 pressing the limit switch 71, the in-place detection mechanism 7 may further include a pressure plate 72. The pressure plate 72 is rotatably connected to the insertion block 9. When the carrier 100 is inserted into the slot 91, it can press against the pressure plate 72 and drive the pressure plate 72 to rotate, thereby pressing the limit switch 71. When the carrier 100 is removed, the limit switch 71 resets and drives the pressure plate 72 to reset. The surface of the pressure plate 72 that contacts the carrier 100 is flat, therefore, after the carrier 100 contacts the pressure plate 72, it is easier for the carrier 100 to continue to be inserted into the preset position.

[0164] Furthermore, a rotating shaft 73 is connected to the limit switch 71, and a mounting plate 74 is connected to one end of the pressure plate 72. The mounting plate 74 has a hole, and the rotating shaft 73 is inserted into the hole, thereby causing the pressure plate 72 to be rotatably connected to the limit switch 71. That is, in this embodiment, the pressure plate 72 is rotatably connected to the insertion block 9 through the limit switch 71. Of course, in other optional embodiments, the rotating shaft 73 connected to the mounting plate 74 can be connected to the insertion block 9.

[0165] like Figure 4 As shown, in order to make as Figure 2a The carrier 100 shown can form good contact with the heating plate 23. Optionally, the nucleic acid amplification detection module 10 may also include a squeezing mechanism 6 for squeezing the squeezing chamber 101 on the carrier 100, and the squeezing mechanism 6 is connected to the temperature regulating mechanism 2.

[0166] Optionally, the extrusion mechanism 6 is located at a position close to the open end of the insertion slot 91, and the extrusion mechanism 6 can partially extend into the insertion slot 91 to extrude the extrusion cavity 101. By extruding the extrusion cavity 101 by the extrusion mechanism 6, the extrusion cavity 101 is compressed and deformed under the extrusion mechanism 6, so that the reaction sample in the containing cavity 103 applies an outward force to the deformable wall, so that the corresponding deformable wall at the containing cavity 103 protrudes outward, and then the corresponding deformable wall at the containing cavity 103 is attached to the temperature adjusting mechanism 2, avoiding the gap between the temperature adjusting mechanism 2 and the deformable wall, and the deformable wall and the temperature adjusting mechanism 2 form good thermal contact, improve the heat conduction efficiency between the deformable wall and the temperature adjusting mechanism 2, realize rapid temperature rise and fall, and improve the amplification efficiency.

[0167] Further, a pressure can be applied to the carrier 100 towards the temperature adjusting mechanism 2 (for example, by applying a pressure to the carrier 100 by the pressing device 5 described below), and under the action of the pressure, the deformable wall can be pressed from the protruding state to the flat state, thereby increasing the contact area between the deformable wall and the temperature adjusting mechanism 2 and improving the heat conduction efficiency between the deformable wall and the temperature adjusting mechanism 2.

[0168] As shown in Figure 8a and Figure 8b , the extrusion mechanism 6 includes an extrusion fixed block, a pressure head 61 and an extrusion elastic member 62, the extrusion fixed block is connected to the temperature adjusting mechanism 2 and is provided with a stepped hole 92, the pressure head 61 is partially arranged in the stepped hole 92 and can abut against the stepped surface of the stepped hole 92, and the other part extends out of the stepped hole 92, and the extrusion elastic member 62 is used to adjust the length of the pressure head 61 extending out of the stepped hole 92.

[0169] As shown in Figure 8a and Figure 8b , and in combination with Figure 5 , in the embodiment, the extrusion fixed block is the insertion block 9, the insertion block 9 is provided with the stepped hole 92 at a position close to the inlet end 911 of the insertion slot 91, and the temperature adjusting mechanism 2 can also be provided with a blind hole 2215, and the extrusion elastic member 62 is contained in the blind hole 2215 and the stepped hole 92. One end of the extrusion elastic member 62 abuts against the pressure head 61, and the other end abuts against the bottom wall of the blind hole 2215 and is always in a compressed state.

[0170] As shown in Figure 8b , optionally, the pressure head 61 has an abutting convex ring 611 in the circumferential direction, one end of the abutting convex ring 611 can abut against the stepped surface of the stepped hole 92, thereby limiting the pressure head 61 and avoiding the pressure head 61 from being separated from the blind hole 2215. The side of the pressure head 61 abutting against the extrusion cavity 101 can be a spherical structure to avoid scratching the deformable wall.

[0171] As shown in Figures 8a-10As shown, the temperature adjusting mechanism 2 comprises a heating sheet 23 for heating the carrier 100 and a cooler 22 for cooling the heating sheet 23, the heating sheet 23 is arranged at one side of the cooler 22 along the first direction X. The heating sheet 23 is always in contact with the cooler 22, and the carrier 100 is always in contact with the heating sheet 23 during amplification. During the amplification stage, the cooler 22 and the heating sheet 23 do not need to move all the time, which saves the driving structure required for moving the two, simplifies the structure of the nucleic acid amplification detection module 10, and enables the nucleic acid amplification detection module 10 to be miniaturized.

[0172] For example, the nucleic acid amplification using the nucleic acid amplification detection module 10 provided in the embodiment comprises the following steps:

[0173] The carrier 100 abuts against the heating sheet 23;

[0174] The cooler 22 keeps cooling all the time to continuously cool the heating sheet 23 and the reaction sample by the cooler 22;

[0175] The temperature increasing process increases the power of the heating sheet 23 to heat the reaction sample to the denaturation temperature or the extension temperature;

[0176] The temperature decreasing process decreases the power of the heating sheet 23 to cool the reaction sample to the annealing temperature;

[0177] Or comprises the following steps:

[0178] The carrier 100 abuts against the heating sheet 23;

[0179] The cooler 22 keeps cooling all the time to continuously cool the heating sheet 23 and the reaction sample by the cooler 22;

[0180] The power of the heating sheet 23 is increased to heat the reaction sample to the denaturation temperature;

[0181] The power of the heating sheet 23 is controlled to keep the reaction sample at the denaturation temperature for a first preset time;

[0182] The temperature decreasing process decreases the power of the heating sheet 23 to cool the reaction sample to the annealing temperature;

[0183] The low-temperature annealing stage adjusts the power of the heating sheet 23 to keep the reaction sample at the annealing temperature for a second preset time;

[0184] The above steps are repeated for multiple times until a preset cycle number or a preset amplification level is reached.

[0185] The temperature increasing process adjusts the power of the heating sheet 23 to heat the reaction sample to the denaturation temperature or the extension temperature.

[0186] In the heating process and the cooling process, the cooler 22 only needs to maintain continuous refrigeration, and the temperature change of the reaction sample is only realized by the change of the heating sheet 23, such as by controlling the power change of the heating sheet 23. In the heating process and the cooling process, the part of the heating sheet 23 close to the cooler 22 is always maintained at a lower temperature. Therefore, when the reaction sample needs to be cooled, the cooler 22 only needs to cool another part of the heating sheet 23 and the reaction sample. That is, because the volume of the heating sheet 23 that needs to be cooled is reduced, the volume of the entire part that needs to be cooled is reduced, thereby shortening the time required for cooling. At the same time, the cooler 22 does not need to perform pre-cooling and moving actions again, thereby achieving the purpose of quickly heating and quickly cooling the reaction sample, and the control method is simple and the control precision is high. At the same time, the cooler 22 and the heating sheet 23 do not need to move throughout the process, and the internal space of the nucleic acid amplification detection module 10 is also saved. Of course, in other optional embodiments, the temperature of the cooler 22 can also be changed accordingly as needed for heating and cooling.

[0187] As shown in Figures 8a-10 , the cooler 22 includes a cooling body 221 and a base 223. The cooling body 221 can circulate cooling liquid to cool the heating sheet 23. The base 223 is provided with a mounting groove 2231, and the cooling body 221 is mounted in the mounting groove 2231. The base 223 facilitates the installation of the cooling body 221.

[0188] As shown in Figure 11 , further, the cooling body 221 includes cooling fins 2211. Two adjacent cooling fins 2211 form a flow channel. The flow channel can circulate liquid cooling medium. The cooling medium continuously flows to take away the heat conducted by the carrier 100 and the heating sheet 23 to the cooling body 221.

[0189] Optionally, the cooling body 221 can further include a cooling shell 2212. The cooling shell 2212 is arranged in the mounting groove 2231. The cooling shell 2212 is provided with a cooling groove. The cooling fins 2211 are arranged in the cooling groove. The cooling shell 2212 and the cooling fins 2211 can be integrally formed by casting or the cooling fins 2211 and the cooling shell 2212 can be connected by welding.

[0190] The cooling shell 2212 can be further provided with a medium port in communication with the cooling groove. The number of the medium ports can be two. The cooling medium enters the cooling groove through one medium port and flows out of the cooling groove through the other medium port.

[0191] Continuing as shown in Figure 8a , Figure 9 and Figure 10As shown, the cooling body 221 can further include a sealing cover 2214, which covers the slot opening of the cooling groove. A sealing ring 2213 can be arranged between the sealing cover 2214 and the cooling shell 2212 to prevent the cooling liquid in the cooling groove from leaking. Optionally, the opening direction of the slot opening of the cooling groove is towards the bottom of the mounting groove 2231 of the base 223, so that the bottom of the mounting groove 2231 of the base 223 can support the sealing cover 2214 after the sealing cover 2214 covers the slot opening of the cooling groove.

[0192] The end surface of the cooling body 221 can be further connected with a heat-conducting temperature measurement plate 222, which is arranged between the heating sheet 23 and the cooling shell 2212. The heat-conducting temperature measurement plate 222 is provided with a temperature measurement groove 2221, and a temperature sensor 224 can be arranged in the temperature measurement groove 2221 to detect the temperature of the cooling body 221 through the temperature sensor 224.

[0193] As shown in Figure 12 , the nucleic acid amplification detection module 10 can further include a shell 3, and the fluorescence detection mechanism 1 and the temperature adjustment mechanism 2 are arranged in the shell 3. The shell 3 is provided with an opening, and the carrier 100 enters and exits the shell 3 through the opening. Further, the carrier 100 is inserted into or taken out of the insertion slot 91 between the temperature adjustment mechanism 2 and the fluorescence detection mechanism 1 through the opening.

[0194] As shown in Figure 12 and Figure 13 , the nucleic acid amplification detection module 10 can further include a cover 4, which can be in an open state of opening the opening or a closed state of closing the opening. The cover 4 can prevent external light from passing through the opening, thereby avoiding affecting the detection structure.

[0195] Optionally, the cover 4 can rotate relative to the shell 3. When the cover 4 is rotated to an approximately horizontal state, the opening is covered, and the cover 4 is in the closed state. When the cover 4 is rotated to an angle with the horizontal direction, the opening is opened, and the cover 4 is in the open state. It can be understood that when the cover 4 is in the open state, the cover 4 can form an acute angle, a right angle or an obtuse angle with the horizontal direction.

[0196] As shown in Figures 14-16As shown, the nucleic acid amplification detection module 10 may further include a clamping device 5, which can be in a clamped state or a released state. When the clamping device 5 is in the clamped state, it clamps the carrier 100 to the temperature regulating mechanism 2; when the clamping device 5 is in the released state, it releases the carrier 100. In this embodiment, the clamping device 5 may also be located inside the housing 3 to facilitate clamping the carrier 100. By changing the state of the clamping device 5 to clamp the carrier to the temperature regulating mechanism, the temperature regulating mechanism no longer needs to move to clamp the carrier. The wiring harness and tubing connected to the temperature regulating mechanism do not need to have the extra length required for the movement of the temperature regulating mechanism. Therefore, the space occupied by the excess wiring harness and tubing can be saved, which facilitates the miniaturization of the nucleic acid amplification detection module 10.

[0197] When the clamping device 5 is in the clamping state, the carrier 100 can contact the temperature regulating mechanism 2, thereby ensuring thermal conductivity. Furthermore, after the clamping mechanism clamps the carrier 100, the extrusion mechanism 6 extrudes the extrusion chamber 101, compressing the air inside and thus extruding the reaction sample inside the receiving chamber 103. The reaction sample extrudes the deformable wall, causing it to adhere tightly to the temperature regulating mechanism 2. It is understood that the temperature regulating mechanism 2 can prevent the deformable wall from protruding outwards. Therefore, even when the reaction sample extrudes the deformable wall, the deformable wall remains flat and adheres tightly to the temperature regulating mechanism 2, ensuring a large contact area between the deformable wall and the temperature regulating mechanism 2, and guaranteeing high thermal conductivity between the reaction sample and the temperature regulating mechanism 2. When the clamping device 5 is in the released state, the carrier 100 can move between the fluorescence detection mechanism 1 and the temperature regulating mechanism 2. More specifically, the carrier 100 can be inserted into or removed from the slot 91.

[0198] like Figures 15-16 As shown, in order to drive the clamping mechanism to switch between the clamping state and the loosening state, the nucleic acid amplification detection module 10 may also include a driving component, which is connected to the clamping device 5 and drives the clamping device 5 to switch between the clamping state and the loosening state.

[0199] The driving component can be a rod, plate, or other part that is easy for the user to hold. In this embodiment, the driving component is the cover 4 mentioned above. That is, the cover 4 is linked with the pressing device 5. The cover 4 can both drive the pressing device 5 and close and open the opening. At the same time, there is no need for a motor or other driving component to drive the pressing device 5, saving space in the nucleic acid amplification and detection module 10 and helping to miniaturize the nucleic acid amplification and detection module 10.

[0200] Further, the cover 4 is switched from the open state to the closed state, and the pressing device 5 is driven from the loosened state to the pressed state; the cover 4 is switched from the closed state to the open state, and the pressing device 5 is driven from the pressed state to the loosened state. That is, when the cover 4 is opened, the cover 4 can drive the pressing device 5 to loosen the carrier 100, so that the operator can take out the carrier 100 after the opening of the opening; after the carrier 100 is inserted, the cover 4 is closed, and the cover 4 can drive the pressing device 5 to press the carrier 100, so that the carrier 100 is tightly attached to the temperature adjusting mechanism 2. The cover 4 is closed synchronously with the pressing of the carrier 100, and the cover 4 is opened synchronously with the loosening of the carrier 100, so that the steps of taking and placing the carrier 100 are simple and the work efficiency is high.

[0201] As shown in Figure 15 , Figure 25 and Figure 30 , optionally, the pressing device 5 comprises a linear mechanism and a pressing mechanism, and the linear mechanism is used to drive the pressing mechanism to translate to press or loosen the carrier 100. Specifically, the linear mechanism drives the pressing mechanism to translate in the first direction X to apply a force in the first direction X to the carrier 100, so that the carrier 100 is pressed to the temperature adjusting mechanism 2. Optionally, the cover 4 is connected with the linear mechanism to drive the linear mechanism.

[0202] It can be understood that, since the carrier 100 is inserted into or taken out of the carrier 100, the pressing mechanism only needs to move a small distance, such as 1.5 mm, in the first direction X, so that the carrier 100 can be inserted into or taken out of the insertion block 9, and thus the pressing mechanism needs a small moving space, which is helpful to realize the miniaturization of the nucleic acid amplification detection module 10.

[0203] As shown in Figures 16-18 , in the embodiment, the linear mechanism is a cam mechanism 51, and the pressing mechanism is a first pressing mechanism 54. The cam mechanism 51 can stably and accurately convert the rotary motion into linear motion in a small moving space, which is convenient for realizing the miniaturization of the nucleic acid amplification detection module 10.

[0204] The cam mechanism 51 comprises a cam 511 and a first guide rod 512, the cam 511 is rotationally connected to the shell 3, the first guide rod 512 is connected to the shell 3 and extends along the first direction X, the first pressing mechanism 54 is slidingly connected to the first guide rod 512, and the cam 511 abuts against the first pressing mechanism 54. It can be understood that the cam 511 has a circumferential surface 5112 abutting against the first pressing mechanism 54, and the circumferential surface 5112 gradually moves away from the rotation center of the cam 511 from one end to the other end in the circumferential direction. The first pressing mechanism 54 can only move along the first direction X under the action of the first guide rod 512, and the first pressing mechanism 54 abuts against different positions on the circumferential surface 5112 of the cam 511 when the cam 511 rotates, so that the first pressing mechanism 54 moves along the first direction X under the driving action of the cam 511 and the guiding action of the first guide rod 512, and then the first pressing mechanism 54 abuts against the carrier 100.

[0205] As shown in FIG. 4, two cams 511 are arranged along the second direction Y, the two cams 511 are connected through a first connecting shaft 516, and the two cams 511 respectively abut against the first pressing mechanism 54, so that the first pressing mechanism 54 is uniformly stressed, and then the pressure of the first pressing mechanism 54 on the carrier 100 is uniform. Figure 17a As shown in FIG. 4, two cams 511 are arranged along the second direction Y, the two cams 511 are connected through a first connecting shaft 516, and the two cams 511 respectively abut against the first pressing mechanism 54, so that the first pressing mechanism 54 is uniformly stressed, and then the pressure of the first pressing mechanism 54 on the carrier 100 is uniform.

[0206] Figure 17a As shown in FIG. 4 and FIG. 5, optionally, the shell 3 can be provided with a fixed component fixedly connected to the shell 3, and the components connected to the shell 3 in the pressing device 5 can be connected to the shell 3 through the fixed component. For example, in order to connect the first guide rod 512 to the shell 3, the fixed component comprises a first fixed plate 517, and the first guide rod 512 is fixedly connected to the first fixed plate 517. Figure 17b As shown in FIG. 4 and FIG. 5, optionally, the first guide rod 512 can be one or more, and in this embodiment, four first guide rods 512 are arranged, and the first guide rods 512 are distributed at the four corners of a square to improve the stability of the movement of the first pressing mechanism 54. In order to rotationally connect the cam 511 to the shell 3, the fixed component can further comprise a shaft support portion, and the cam 511 can be fixedly connected to the first connecting shaft 516 to drive the two cams 511 to rotate synchronously, the shaft support portion is provided with a shaft hole, and the first connecting shaft 516 is arranged in the shaft hole to be rotationally connected to the shaft support portion. Specifically, the shaft support portion comprises an installation block 518 and a second fixed plate 519 connected to each other, and the installation block 518 and the second fixed plate 519 are both provided with an arc-shaped groove on the side facing each other, and the two arc-shaped grooves can be spliced into a shaft hole after the installation block 518 and the second fixed plate 519 are connected.

[0207] ​Optionally, the second connecting shaft 526 is connected with a shaft support at both axial ends, so as to improve the stability of the rotation of the second connecting shaft 526. For example, the pressing device 5 is arranged in the shell, and the lower end of the second fixed plate 519 is connected to the shell 3. In order to improve the stability of the second fixed plate 519 and the first fixed plate 517, the side surface of the second fixed plate 519 can be connected to the first fixed plate 517.

[0208] In order to drive the cam 511 to rotate, the cover 4 is connected with a transmission part 515, one end of the transmission part 515 is connected to the cam 511, so as to drive the cam 511 to rotate.

[0209] As shown in Figure 17a and Figure 18 , further, the cam 511 is provided with a U-shaped groove 5111, and one end of the transmission part 515 is slidingly and rotatably arranged in the U-shaped groove 5111. It can be understood that the opening of the U-shaped groove 5111 is generally directed to the cover 4, so that one end of the transmission part 515 enters the U-shaped groove 5111. The transmission part 515 can include a transmission body 5151 and a pulley 5152 connected to the transmission body 5151, one end of the transmission body 5151 is fixedly connected to one end of the cover 4, so as to drive the transmission body 5151 to rotate when the cover 4 rotates. The pulley 5152 is connected to the other end of the transmission body 5151, and the pulley 5152 can reduce the friction with the groove wall of the U-shaped groove 5111, so as to avoid the wear of the pulley 5152 and the cam 511. It can be understood that the depth of the U-shaped groove 5111 can be set according to the sliding distance of the pulley 5152 in the U-shaped groove 5111, so that the pulley 5152 is always located in the U-shaped groove 5111.

[0210] As shown in Figure 17a , Figure 19a , Figure 19b and Figure 20 , the cam mechanism 51 can further include a first locking piece 513, the first locking piece 513 is arranged on the first guide rod 512 and abuts against the first pressing mechanism 54, so as to make the first pressing mechanism 54 always abut against the cam 511. As shown in Figure 19a , for example, the first locking piece 513 abuts against the first pressing mechanism 54, so as to apply a leftward force to the first pressing mechanism 54, thereby making the first pressing mechanism 54 always abut against the cam 511.

[0211] Continuing to refer to Figure 17a , and combining Figure 19aAs shown, one end of the first guide rod 512 is connected to a first flange 514. The first locking member 513 includes a spring, which is sleeved on the first guide rod 512. One end of the spring abuts against the first flange 514, and the other end abuts against the first pressing mechanism 54. It can be understood that the spring is always in a compressed state to apply pressure towards the cam 511 to the first pressing mechanism 54. Figure 19a The force acting on the left side of the image. For example... Figure 19a As shown, exemplarily, the first pressing mechanism 54 has a guide step hole 5411, the first guide rod 512 passes through the guide step hole 5411, and the spring is disposed inside the guide step hole 5411. One end of the spring abuts against the stepped surface of the guide step hole 5411, and the other end abuts against the first flange 514. In other optional embodiments, the first pressing mechanism 54 may not have a guide step hole 5411, or it may have a through hole with the same diameter. The first guide rod 512 passes through the through hole, and the spring is located outside the through hole. One end of the spring abuts against the surface of the first pressing mechanism 54, and the other end abuts against the first flange 514.

[0212] like Figures 19a-19c As shown, the first pressing mechanism 54 includes a pressing frame 541 and a first pressing plate 542. One end of the spring presses against the pressing frame 541, and the other end presses against the first flange 514.

[0213] like Figure 17a and Figure 18 As shown, a guide step hole 5411 is formed in the clamping frame 541, and the clamping frame 541 is slidably connected to the first guide rod 512, thereby causing the first clamping mechanism 54 to translate along the first direction X. The first clamping plate 542 is elastically connected to the clamping frame 541, thereby causing the first clamping plate 542 to elastically contact the carrier 100, avoiding damage to the first clamping mechanism 54 after long-term use, and avoiding damage to the carrier 100.

[0214] It is understood that the first pressing plate 542 is located between the temperature regulating mechanism 2 and the fluorescence detection mechanism 1, so that the movement of the first pressing plate 542 in the first direction X can press or release the carrier 100. Figure 22 As shown, a first clearance hole 5421 is provided on the first pressing plate 542. The fluorescence detection mechanism 1 detects the reaction sample through the first clearance hole 5421, so that the carrier 100 can be pressed against the temperature adjustment mechanism 2 and fluorescence detection can be realized.

[0215] The first pressing mechanism 54 may also include a pressing wheel 543 for abutting against the cam 511. The pressing wheel 543 is rotatably connected to the pressing frame 541, thereby greatly reducing the wear generated when the first pressing mechanism 54 and the cam 511 abut against each other.

[0216] like Figure 19b , Figure 19c andFigure 20 As shown, further, the first pressing mechanism 54 can further include a second guide rod 545 connected to the pressing frame 541 and extending along the first direction X, and the first pressing plate 542 is slidingly connected to the second guide rod 545. To enable the first pressing plate 542 to be slidingly connected to the second guide rod 545, the first pressing plate 542 can optionally be provided with a sliding through hole 5422, and the second guide rod 545 passes through the sliding through hole 5422, and the end of the second guide rod 545 has a flange that can abut against the first pressing plate 542, thereby preventing the first pressing plate 542 from falling off the second guide rod 545.

[0217] Optionally, the second guide rod 545 can be one or more, and in the embodiment, four second guide rods 545 are provided, which are distributed at the four corners of the square to improve the stability of the movement of the first pressing plate 542.

[0218] Optionally, a first buffer 544 is provided between the pressing frame 541 and the first pressing plate 542, so that the pressing frame 541 and the first pressing plate 542 are elastically connected, and the first pressing plate 542 and the carrier 100 are in elastic contact, and at the same time, the first buffer 544 can also eliminate the machining and installation errors of the first pressing plate 542, fine-tune the angle of the first pressing plate 542, and ensure that the first pressing plate 542 is pressed to the carrier 100 at all places.

[0219] Illustratively, the first buffer 544 is a spring, which is sleeved outside the second guide rod 545 and located between the pressing frame 541 and the first pressing plate 542, so that the first pressing plate 542 and the pressing frame 541 are elastically connected, and at the same time, the angle of the first pressing plate 542 is fine-tuned, and it is ensured that the first pressing plate 542 is pressed to the carrier 100 at all places, and that the first pressing plate 542 moves to press the carrier 100 along the first direction X.

[0220] As Figure 20 shown, it can be understood that when the spring is compressed, the end of the second guide rod 545 will protrude from the first pressing plate 542, in order to avoid the second guide rod 545 affecting the pressing of the carrier 100 by the first pressing plate 542, as Figure 8a shown, after the carrier 100 is inserted into the insertion block 9, it can protrude from the insertion block 9 in the first direction X, so that after the carrier 100 is pressed by the first pressing plate 542, the end of the second guide rod 545 can be accommodated in the space between the insertion block 9 and the first pressing plate 542.

[0221] When the carrier 100 needs to be pressed, the cover 4 is closed, the cover 4 drives the transmission part 515 to rotate, the pulley 5152 slides in the U-shaped groove 5111, and the cam 511 is driven to rotate, the cam 511 rotates and overcomes the force of the first locking part 513, drives the first pressing mechanism 54 to move in the first direction X (moves to the right as shown in the figure), and then presses the carrier 100 on the temperature adjusting mechanism 2. Figure 20 When the carrier 100 needs to be pressed, the cover 4 is closed, the cover 4 drives the transmission part 515 to rotate, the pulley 5152 slides in the U-shaped groove 5111, and the cam 511 is driven to rotate, the cam 511 rotates and overcomes the force of the first locking part 513, drives the first pressing mechanism 54 to move in the first direction X (moves to the right as shown in the figure), and then presses the carrier 100 on the temperature adjusting mechanism 2. Figure 20 When the carrier 100 needs to be pressed, the cover 4 is closed, the cover 4 drives the transmission part 515 to rotate, the pulley 5152 slides in the U-shaped groove 5111, and the cam 511 is driven to rotate, the cam 511 rotates and overcomes the force of the first locking part 513, drives the first pressing mechanism 54 to move in the first direction X (moves to the right as shown in the figure), and then presses the carrier 100 on the temperature adjusting mechanism 2. Figure 20 When the carrier 100 needs to be pressed, the cover 4 is closed, the cover 4 drives the transmission part 515 to rotate, the pulley 5152 slides in the U-shaped groove 5111, and the cam 511 is driven to rotate, the cam 511 rotates and overcomes the force of the first locking part 513, drives the first pressing mechanism 54 to move in the first direction X (moves to the right as shown in the figure), and then presses the carrier 100 on the temperature adjusting mechanism 2.

[0222] As shown in Figure 19b and Figure 20 , in order to facilitate detection of whether the pressing device 5 presses the carrier 100, the nucleic acid amplification detection module 10 can also optionally include a pressing detection mechanism 57, which is used to detect whether the pressing mechanism presses the carrier 100.

[0223] Specifically, the pressing detection mechanism 57 includes a photoelectric switch 571 and a blocking piece 572, one of which is connected to the pressing mechanism, and the other is fixedly connected to the shell 3. The first pressing mechanism 54 translates and can make the blocking piece 572 enter and exit between the emitting part and the receiving part of the photoelectric switch 571. For example, in the embodiment, the photoelectric switch 571 is connected to the shell 3, and the blocking piece 572 is connected to the first pressing mechanism 54, as shown in Figure 21a Further, the blocking piece 572 is connected to the pressing frame 541. For example, when the blocking piece 572 is between the emitting part and the receiving part of the photoelectric switch 571, the blocking piece 572 blocks the light, and the receiving part cannot receive the signal, at this time the first pressing mechanism 54 does not press the carrier 100 (or presses the carrier 100); when the blocking piece 572 leaves the photoelectric switch 571, the receiving part receives the signal, at this time the first pressing mechanism 54 presses the carrier 100 (or does not press the carrier 100).

[0224] As shown in Figure 21a and Figure 21b , the pressing frame 541 is a ring frame, such as a square ring. The cooler 22 or the fluorescence detection mechanism 1 described above can be arranged in the ring frame, so that the structure of the nucleic acid amplification detection module 10 is more compact.

[0225] Specifically, as shown in Figure 22 and Figure 23 , and in combination with Figure 17aIn the present embodiment, the fluorescence detection mechanism 1 is arranged in the pressing device 5, and part of the fluorescence detection mechanism 1 is arranged in the square frame. As shown in Figure 23 The cam 511 is arranged at one side of the fluorescence detection mechanism 1 along the second direction Y, so that the cam 511 reduces or avoids the space of the cam 511 along the first direction X using the nucleic acid amplification detection module 10, thereby reducing the size of the nucleic acid amplification detection module 10 along the first direction X. When the pressing device 5 is provided with two cams 511 along the second direction Y, the fluorescence detection mechanism 1 is located between the two cams 511.

[0226] In combination Figure 1 Since the size of the temperature adjusting mechanism 2 along the second direction Y is greater than the size of the fluorescence detection mechanism 1 along the second direction Y, there is a free space on both sides of the fluorescence detection mechanism 1 along the second direction Y. In the present embodiment, the pressing device 5 occupies the free space on at least one side of the fluorescence detection mechanism 1 along the second direction Y (as shown in Figure 1 Therefore, the space in the nucleic acid amplification detection module 10 is fully and reasonably utilized, which is beneficial to the miniaturization of the nucleic acid amplification detection module 10.

[0227] As shown in Figure 24a When the fluorescence detection mechanism 1 is arranged in the pressing device 5, the size of the fluorescence detection mechanism 1 and the pressing device 5 at the corresponding second direction Y is substantially the same as the size of the temperature adjusting mechanism 2 at the corresponding second direction Y, that is, after the pressing device 5 is added, the size of the nucleic acid amplification detection module 10 along the second direction Y is basically not increased. In the case where the shell 3 is not arranged, the size of the nucleic acid amplification detection module 10 along the first direction X, the second direction Y and the vertical direction Z is only 191 mm x 60 mm x 140 mm.

[0228] As shown in Figure 24b Of course, in other optional embodiments, the temperature adjusting mechanism 2 can be arranged in the pressing device 5 (not shown in the figure), and the temperature adjusting mechanism 2 is located between the two cams 511. At this time, after the carrier 100 is inserted into the shell 3 and located in the pressing frame 541, the first locking member 513 applies a left force to the pressing frame 541, so that the first pressing mechanism 54 moves to the left as shown in Figure 20 The cam 511 drives the first pressing mechanism 54 to move to the right as shown in Figure 20 The cam 511 drives the first pressing mechanism 54 to move to the right as shown in Embodiment Two

[0229] The present embodiment two is basically the same as the embodiment one, and the main difference between the two is the specific structure of the pressing device, as shown in Figures 25-27 In the present embodiment, the linear mechanism is a gear mechanism 52, and the pressing mechanism corresponds to a second pressing mechanism 55.

[0230] Specifically, the gear mechanism 52 includes a driving gear 521, a driven gear 522 rotatably connected to the housing 3, and a transmission assembly 523. The driven gear 522 has an arc-shaped guide hole 5211, which gradually moves away from the center of the driven gear 522 from one end to the other (e.g., ...). Figure 25 As shown, the arc-shaped guide hole 5211 gradually approaches the center of the driven gear 522 in a clockwise direction. The transmission component 523 is slidably connected to the housing 3 in the first direction X. One end of the transmission component 523 is slidably disposed in the arc-shaped guide hole 5211, and the other end is connected to the second pressing mechanism 55 to drive the second pressing mechanism 55 to translate.

[0231] Optionally, the cover 4 is connected to the driven gear 522. The rotation of the cover 4 drives the driven gear 522 to rotate, thereby realizing the linkage between the cover 4 and the pressing device 5.

[0232] In this embodiment, the temperature regulating mechanism 2 is located in the pressing device 5 as an example for explanation. Figures 25-28 As shown, when the cover 4 is closed, the transmission assembly 523 slides along the arc-shaped guide hole 5211 and gradually approaches the center of the driven gear 522. The transmission assembly 523 drives the second pressing mechanism 55 to move towards the side closer to the carrier 100 (e.g., Figure 25 (As shown, move to the left) until the cover 4 is tightly closed, and the second pressing mechanism 55 presses the carrier 100. Figure 26 As shown, when the cover 4 is opened, the transmission assembly 523 slides along the arc-shaped guide hole 5211 and gradually moves away from the center of the driven gear 522. The transmission assembly 523 drives the second pressing mechanism 55 to move away from the carrier 100 (e.g., Figure 25 (as shown, move to the right) until the cover 4 is opened, and the second clamping mechanism 55 releases the carrier 100.

[0233] like Figure 26 and Figure 27 As shown, in this embodiment, the gear mechanism 52 may further include a fixed guide 527, which is fixedly connected to the housing 3. The transmission assembly 523 is slidably connected to the fixed guide 527 along the first direction X. The fixed guide 527 can provide guidance for the transmission assembly 523, causing the transmission assembly 523 to reciprocate along the first direction X, thereby driving the second pressing mechanism 55 to reciprocate along the first direction X, thereby pressing or releasing the carrier 100.

[0234] like Figure 27As shown, the gear mechanism 52 can further comprise a gear fixing member 529, the driven gear 522 can be rotatably connected to the gear fixing member 529, and the gear fixing member 529 is directly or indirectly connected to the housing 3, so that the driven gear 522 rotates relative to the housing 3.

[0235] As shown in Figure 27 and Figure 28 , the driven gear 522 and the transmission assembly 523 are arranged on one side of the temperature adjusting mechanism 2 along the second direction Y.

[0236] Alternatively, two groups of transmission assemblies 523 are arranged along the second direction Y, and the two groups of transmission assemblies 523 are connected by a second connecting shaft 526, the second connecting shaft 526 is slidingly arranged in the arc-shaped guide hole 5211, so that the two groups of transmission assemblies 523 slide synchronously relative to the arc-shaped guide hole 5211. The two groups of transmission assemblies 523 apply pressure force to the second pressing mechanism, which can make the second pressing mechanism 55 and the carrier 100 bear force uniformly. At this time, the temperature adjusting mechanism 2 is located between the two groups of transmission assemblies 523, and the temperature adjusting mechanism 2 is located in the space surrounded by the two groups of transmission assemblies 523 and the second pressing plate 551, so that the temperature adjusting mechanism 2 is located in the pressing device 5.

[0237] As shown in Figure 27 , the fixed guide 527 is a U-shaped structure, thereby guiding the two groups of transmission assemblies 523.

[0238] As shown in Figure 29a , optionally, the transmission assembly 523 comprises a transmission member 5231 and a fourth guide rod 5232 connected to each other, wherein one end of the transmission member 5231 is provided with an axle hole 5233, the second connecting shaft 526 is arranged in the axle hole 5233 and is slidingly connected with the arc-shaped guide hole 5211, and the fourth guide rod 5232 is slidingly arranged in the fixed guide 527.

[0239] As shown in Figure 26 , when the transmission assembly 523 moves to the right as shown in Figure 26 , the left wall of the arc-shaped guide hole 5211 applies a rightward force to the second connecting shaft 526; conversely, when the transmission assembly 523 moves to the left as shown in Figure 26When the left side of the arc-shaped guide hole 5211 is shown, the right side wall of the arc-shaped guide hole 5211 applies a leftward force to the second connecting shaft 526. It can be understood that the second connecting shaft 526 slides relative to the arc-shaped guide hole 5211, and the radial dimension of the arc-shaped guide hole 5211 along the driven gear 522 is greater than the diameter of the second connecting shaft 526, so that the second connecting shaft 526 repeatedly collides with the left side wall and the right side wall of the arc-shaped guide hole 5211 during the reciprocating movement of the transmission assembly 523 in the first direction X, which can easily cause wear of the second connecting shaft 526 and the arc-shaped guide hole 5211, and further can cause the second compression assembly to fail to compress the carrier 100.

[0240] To solve the above technical problems, optionally, the gear mechanism 52 can further include a second locking member 524, which is arranged between the transmission assembly 523 and the second compression mechanism 55 and abuts against the second compression mechanism 55, and the second locking member 524 is used to make the transmission assembly 523 always abut against one side of the inner wall of the arc-shaped guide hole 5211. Specifically, in the embodiment, the second locking member 524 is arranged between the fixed guide member 527 and the second compression mechanism 55, and always applies a force to the second compression mechanism 55 to make the second compression mechanism 55 drive the transmission assembly 523 to always abut against one side of the arc-shaped guide hole 5211 (for example, the right side of the arc-shaped guide hole 5211 as shown in the figure). Figure 26

[0241] Figure 26 Figure 27 Further, the second locking member 524 includes a spring, the spring is sleeved on the fourth guide rod 5232 of the transmission assembly 523 and is located between the fixed guide member 527 and the second compression mechanism 55, and further, one end of the spring abuts against the fixed guide member 527 and the other end abuts against the second compression mechanism 55. It can be understood that the spring is always in a compressed state to always apply a force to the second compression mechanism 55 towards the right side as shown in the figure, so that the second connecting shaft 526 always abuts against the wall of the right side of the arc-shaped guide hole 5211, thereby avoiding the second connecting shaft 526 repeatedly colliding with the wall of the arc-shaped guide hole 5211 in the first direction X. Figure 26

[0242] Figure 26 Figure 27 ​​​​​​As shown, the second pressing mechanism 55 comprises a second pressing plate 551, the fourth guide rod 5232 of the transmission assembly 523 slides through the second pressing plate 551, and the second pressing plate 551 is used to press the carrier 100. Wherein, the other end of the spring abuts against the second pressing plate 551, the second pressing plate 551 is provided with a second avoiding hole 5511, and the fluorescence detection mechanism 1 detects the reaction sample through the second avoiding hole 5511. It can be understood that the second pressing plate 551 is located between the temperature adjusting mechanism 2 and the fluorescence detection mechanism 1, so that the movement of the second pressing plate 551 in the first direction X can press or release the carrier 100.

[0243] The second pressing mechanism 55 has simple structure and does not affect the detection of the fluorescence detection mechanism 1.

[0244] Optionally, a second buffer 528 is arranged between the gear mechanism 52 and the second pressing mechanism 55. When the gear mechanism 52 drives the second pressing mechanism 55 to move until pressing the carrier 100, the second buffer 528 can make the second pressing mechanism 55 and the carrier 100 elastically contact, and at the same time, the second buffer 528 can eliminate the machining error and installation error of the second pressing plate 551, fine-tune the angle of the second pressing plate 551, and ensure that the second pressing plate 551 is pressed to the carrier 100.

[0245] As shown in the figure, Figure 26 exemplarily, the other end of the transmission assembly 523 is connected with a second flange 525, the second buffer 528 is a spring, the spring is sleeved outside the fourth guide rod 5232 of the transmission assembly 523, and is located between the second flange 525 and the second pressing plate 551.

[0246] As shown in the figure, Figure 25 when the carrier 100 is pressed, under the action of the arc-shaped guide hole 5211, the transmission assembly 523 and the second flange 525 are driven to move to the left as shown in the figure, Figure 25 the second flange 525 applies a rightward force to the second pressing plate 551 through the second buffer 528, at the same time, the second buffer 528 overcomes the force applied to the second pressing plate 551 by the second locking piece 524, so that the second pressing plate 551 moves to the left as shown in the figure, Figure 25 until pressing the carrier 100.

[0247] As shown in the figure, Figure 26 when the carrier 100 is released, under the action of the arc-shaped guide hole 5211, the transmission assembly 523 and the second flange 525 are driven to move to the right as shown in the figure, Figure 26 initially, the second buffer 528 releases a certain force, and the pressure on the carrier 100 gradually decreases, the transmission assembly 523 and the second flange 525 continue to move to the right as shown in the figure, Figure 26As shown in the rightward movement, the force exerted by the second buffer 528 on the second pressing plate 551 is further reduced and becomes less than the force exerted by the second locking member 524 on the second pressing plate 551. Therefore, under the action of the second locking member 524, the second pressing plate 551 is driven to separate from the carrier 100.

[0248] It is understandable that in this embodiment, the travel distance of the clamping device 5 along the first direction X is relatively short, for example, approximately 1.5 mm. Simultaneously, when the second clamping plate 551 clamps the carrier 100, the pressure of the second buffer member 528 on the second clamping plate 551 needs to be greater than the pressure of the second locking member 524 on the second clamping plate 551. Furthermore, during the process of releasing the carrier 100, the pressure of the second buffer member 528 on the second clamping plate 551 needs to be less than the pressure of the second locking member 524 on the second clamping plate 551. Therefore, the second buffer member 528 needs to have a large force change under small deformation, i.e., the stiffness coefficient of the second buffer member 528 needs to be large. This increases the cost of the clamping device 5 and narrows the selection range of the second buffer member 528.

[0249] Therefore, to solve the above-mentioned technical problems, the second pressing mechanism 55 may optionally include a pre-pressing block 552 for pre-pressing the second buffer member 528. The pre-pressing block 552 is connected to the second pressing plate 551. Specifically, the pre-pressing block 552 has a receiving groove 553. The second buffer member 528 and the second flange 525 are disposed in the receiving groove 553. The second flange 525 can slide relative to the pre-pressing block 552 to increase the deformation space of the second buffer member 528, so that the second pressing plate 551 can have a large deformation space with a small movement space.

[0250] like Figure 25 As shown, when the cover 4 is closed, the second pressing plate 551 presses the carrier 100, the second flange 525 and the second pressing plate 551 press the second buffer 528, the force of the second locking member 524 is less than the force of the second buffer 528, part of the force of the second buffer 528 resists the second locking member 524, and the other part of the force presses the carrier 100.

[0251] like Figure 26 As shown, when the cover 4 is opened, the transmission assembly 523, under the action of the second buffer 528, moves in a directional manner as follows: Figure 26 As shown in the rightward movement trend, the second connecting shaft 526 approaches the right side wall of the arc-shaped guide hole 5211, and the transmission assembly 523 moves to the right. Continuing to open the cover 4, the transmission assembly 523, under the action of the second buffer 528, moves to the right until the second flange 525 abuts against the bottom of the receiving groove 553 of the pre-pressing block 552 (as shown). Figure 26the right side of the accommodating groove 553). At this time, the second buffer 528 can no longer continue to stretch, and the second flange 525 is in contact with the pre-pressing block 552 and is deadlocked, and the elastic force of the second buffer 528 no longer has the effect of pushing the second flange 525 to the right. After the second buffer 528 no longer functions, the second locking member 524 begins to stretch, pushing the second pressing plate 551 to the right as shown in Figure 26 the right side movement, at this time, since the transmission assembly 523 is pressed against the bottom of the accommodating groove 553 by the second buffer 528, the second pressing plate 551 and the pre-pressing block 552 continue to move along the first direction X as shown in Figure 26 the right side movement, until the carrier 100 is released.

[0252] As shown in Figure 29b It can be understood that in other alternative embodiments, the fluorescence detection mechanism 1 can also be located in the pressing device 5, so as to utilize the Figure 1 As shown in the empty space, the fluorescence detection mechanism 1 is located between the two sets of transmission assemblies 523. At this time, the arrangement of the arc-shaped guide hole 5211 is changed accordingly (i.e., as shown in Figure 25 the clockwise direction, the arc-shaped guide hole 5211 gradually moves away from the center of the driven gear 522), so as to achieve the purpose of closing the cover 4, pressing the carrier 100, opening the cover 4, and releasing the carrier 100. Further, when pressing the carrier 100, the second flange 525 is driven by the transmission assembly 523 to move to the right as shown in Figure 26 the right side, and further drives the pre-pressing block 552 and the second pressing plate 551 to move to the right as shown in Figure 26 the right side, and drives the second pressing plate 551 to press the carrier 100 through the second locking member 524; when releasing the carrier 100, the second flange 525 is driven by the transmission assembly 523 to move to the left as shown in Figure 26 the left side, and further drives the third pressing plate to move to the left as shown in Figure 26 the left side to release the carrier 100.

[0253] Compared with the straight line mechanism in Embodiment One, the present embodiment requires a driving gear 521 and a driven gear 522, but compared with the cam 511 in Embodiment One, the machining cycle of the gear is longer and the machining cost is higher. Embodiment Three

[0254] As shown in Figures 30-32 The present embodiment three is basically the same as Embodiment One, and the main difference between the two is that the specific structure of the pressing device is different. In the present embodiment, the straight line mechanism is a crank slider mechanism 53, and the pressing mechanism corresponds to a third pressing mechanism 56.

[0255] The crank slider mechanism 53 comprises a connecting rod 531, a slider 532 and a third guide rod 533 connected to the shell 3 and extending along the first direction X, the slider 532 is sleeved on the third guide rod 533, one end of the connecting rod 531 is rotatably connected to the slider 532 and drives the slider 532 to slide along the third guide rod 533, and the slider 532 is used to drive the third pressing mechanism 56 to press against the carrier 100.

[0256] As shown in Figure 30 , the connecting rod 531 is rotatably connected to the cover 4, the cover 4 drives the connecting rod 531 to rotate, thereby realizing the linkage between the cover 4 and the pressing device 5. It can be understood that the rotation axis of the cover 4 does not coincide with the axis of the connecting rod 531 and the cover 4, so as to realize the sliding of the slider 532 along the third guide rod 533. As shown in Figure 30 , when the cover 4 is closed, the third pressing mechanism 56 presses the carrier 100, as shown in Figure 31 , when the cover 4 is opened, the third pressing mechanism 56 releases the carrier 100.

[0257] As shown in Figure 32 , the slider 532 and the third guide rod 533 are arranged on one side of the fluorescence detection mechanism 1 along the second direction Y, and the fluorescence detection mechanism 1 is located in the pressing device 5. In this embodiment, the fluorescence detection mechanism 1 is located in the crank slider mechanism 53, as shown in Figure 30 and Figure 31 , the slider 532 moves to the right along the third guide rod 533, pushing the third pressing plate 562 to press the carrier 100, and the slider 532 moves to the left along the third guide rod 533, and the third pressing mechanism 56 can release the carrier 100.

[0258] As shown in Figure 32 , the third pressing mechanism 56 comprises a pressing rod 561 rotatably connected to the third guide rod 533, and the end of the third guide rod 533 can be connected to a third flange 535 to prevent the pressing rod 561 from sliding out of the third guide rod 533.

[0259] The third pressing mechanism 56 can further comprise a third pressing plate 562, and the third pressing plate 562 is provided with a third avoiding hole 5621, and the fluorescence detection mechanism 1 detects the reaction sample through the third avoiding hole 5621. The third pressing plate 562 is connected with the pressing rod 561 to move along the first direction X with the pressing rod 561. As shown in Figure 31 , the third pressing plate 562 is located between the temperature adjusting mechanism 2 and the fluorescence detection mechanism 1, so that the third pressing plate 562 can press or release the carrier 100 when moving along the first direction X.

[0260] To make the elastic contact between the slider 532 and the third pressing mechanism 56, the third buffer 536 is arranged between the slider 532 and the third pressing mechanism 56. Optionally, the third buffer 536 is a spring, which is sleeved on the third guide rod 533 and located between the third pressing mechanism 56 and the slider 532. The third buffer 536 can make the elastic contact between the third pressing mechanism 56 and the carrier 100, and at the same time, the third buffer 536 can also eliminate the machining and installation errors of the third pressing plate 562, fine-tune the angle of the third pressing plate 562, and ensure that the third pressing plate 562 is tightly pressed on the carrier 100.

[0261] As shown in Figure 31 It can be understood that when the connecting rod 531 drives the slider 532 to move to the right along the third guide rod 533, the third pressing mechanism 56 can be driven to move to the right by abutting against the third pressing mechanism 56, and then the carrier 100 is pressed. When the slider 532 moves to the left, in order to make the third pressing mechanism 56 also move to the left, the slider 532 and the third pressing mechanism 56 can be connected, such as one end of the third buffer 536 being connected with the slider 532 and the other end being connected with the third pressing mechanism 56.

[0262] However, in the embodiment, in order to ensure the result of the fluorescence detection, the distance between the fluorescence detection mechanism 1 and the carrier 100 needs to be ensured within a predetermined range, so the stroke of the third pressing plate 562 is short, and when the cover 4 rotates, the slider 532 needs to slide a large stroke, which causes the third buffer 536 to be stretched for a large length, and the third buffer 536 needs to be repeatedly stretched and compressed, so the third buffer 536 is easy to fail.

[0263] As shown in Figure 32 Therefore, to solve the above technical problems, in the embodiment, the slider 532 and the third pressing mechanism 56 are not connected, so that the third pressing plate 562 of the third pressing mechanism 56 is driven to loosen the carrier 100, the crank slider mechanism 53 further comprises a third locking piece 534, the third locking piece 534 is arranged on the third guide rod 533 and abuts against the third pressing mechanism 56, and the third locking piece 534 drives the third pressing mechanism 56 to move to the side where the slider 532 is located. When the slider 532 slides to the left as shown in Figure 31 As shown in Figure 31 The third locking piece 534 can drive the third pressing mechanism 56 to move to the left as shown in

[0264] Optionally, the third locking piece 534 is a spring, which is sleeved on the third guide rod 533 and abuts against the pressing rod 561 and the third flange 535, that is, the spring is located between the pressing rod 561 and the third flange 535.

[0265] As shown in Figures 30-32As shown, when the pressing device 5 provided by the embodiment is used, as Figure 30 and shown in Figure 32 When the cover 4 is opened, the third locking member 534 and the third buffer member 536 are in a natural state. When the cover 4 is closed, the cover 4 rotates to drive the connecting rod 531 to rotate, and the connecting rod 531 drives the sliding block 532 to move to the right as shown in Figure 30 until the sliding block 532 abuts against one end of the third buffer member 536. The sliding block 532 continues to move, the third buffer member 536 is compressed, and exerts a rightward force on the pressing rod 561 as shown in Figure 30 , and the third locking member 534 is compressed until the third pressing plate 562 is pressed against the carrier 100; as Figure 31 and shown in Figure 31 When the cover 4 is opened, the cover 4 rotates to drive the connecting rod 531 to rotate, and the connecting rod 531 drives the sliding block 532 to move to the left as shown in Figure 31 , and the third buffer member 536 gradually returns to the natural state, so the force on the third pressing mechanism 56 and the third locking member 534 gradually decreases, and the third locking member 534 gradually returns to the natural state while driving the third pressing mechanism 56 to move to the right as shown in Figure 32 , thereby releasing the carrier 100.

[0266] As shown in Figure 1 , two groups of sliding blocks 532 and third guide rods 533 are arranged along the second direction Y. The two groups of sliding blocks 532 are connected by a third connecting shaft, the connecting rod 531 is connected to the third connecting shaft (not shown in the figure), and the connecting rod 531 is fixedly connected to the third connecting shaft to simultaneously drive the two sliding blocks 532 to slide. The two groups of sliding blocks 532 and the third guide rods 533 can make the third pressing mechanism 56 and the carrier 100 bear forces uniformly. Optionally, two groups of pressing rods 561 are connected to the third pressing plate 562, and the two groups of pressing rods 561 are arranged in the second direction Y to be connected to the two groups of third guide rods 533, respectively. The fluorescence detection mechanism 1 is located between the two groups of third guide rods 533. It can be understood that the fluorescence detection mechanism 1 is located in the space surrounded by the two groups of third guide rods 533 and the third pressing plate 562, so as to make full use of the empty space shown in Figure 31 .

[0267] Optionally, the pressing rod 561 includes a first rod 5611 and a second rod 5612 connected in an L shape. The first rod 5611 extends along the second direction Y and is slidingly connected to the third guide rod 533. The second rod 5612 extends along the first direction X and is connected to the third pressing plate 562. The pressing plate of this structure can avoid the third flange 535, and facilitate abutment of the third locking member 534, the third buffer member 536, and the third pressing mechanism 56.

[0268] It can be understood that in other optional embodiments, the slider 532 and the third guide rod 533 can be arranged on one side of the temperature adjusting mechanism 2 along the second direction Y, and the temperature adjusting mechanism 2 is located in the pressing device 5. At this time, the third pressing mechanism 56 is pressed by applying a leftward (with reference to Figure 31 ) force to the third pressing mechanism 56 through the third locking member 534; and the third pressing mechanism 56 is released by applying a rightward (with reference to Figure 33 ) force to the third pressing mechanism 56 through the slider 532 and the third buffer 536. Embodiment Four

[0269] As shown in ​ , the embodiment discloses a nucleic acid amplification and detection integrated machine, which comprises the nucleic acid amplification and detection module 10 in the embodiment one, the embodiment two or the embodiment three, so as to amplify and detect the reaction sample, realize the miniaturization of the nucleic acid amplification and detection integrated machine, and improve the detection efficiency.

[0270] The nucleic acid amplification and detection integrated machine comprises one or more groups of nucleic acid amplification and detection modules 10. In the embodiment, the nucleic acid amplification and detection integrated machine is provided with four groups of nucleic acid amplification and detection modules 10. Optionally, the four groups of nucleic acid amplification modules are sequentially arranged along the second direction Y, so as to improve the structural compactness of the nucleic acid amplification and detection integrated machine, and facilitate the miniaturization thereof. Of course, the number of the nucleic acid amplification and detection modules 10 is not limited to four groups, and can be more than four groups or less than four groups.

[0271] The nucleic acid amplification and detection integrated machine can further comprise a water cooler 20, which is used for providing the cooling medium for the temperature adjusting mechanism 2. For example, the outlet of the water cooler 20 is connected with the medium inlet 2216 of the cooler 22, and the inlet of the water cooler 20 is connected with the medium outlet 2217 of the cooler 22, so that the cooling medium circulates between the water cooler 20 and the cooler 22. Optionally, the water cooler 20 is arranged below the nucleic acid amplification and detection module 10.

[0272] The nucleic acid amplification and detection integrated machine can further comprise a control module 30, which is electrically connected with the water cooler 20 and the nucleic acid amplification and detection module 10, so as to control the water cooler 20 and the nucleic acid amplification and detection module 10. Optionally, the control module 30 is arranged on the upper side of the water cooler 20 and located on one side of the nucleic acid amplification module along the first direction X. The nucleic acid amplification and detection integrated machine has a reasonable and compact structure, and is convenient for miniaturization.

[0273] Although the present application has been described in detail with general description, specific embodiments and experiments, some modifications or improvements can be made on the basis of the present application, which is obvious to those skilled in the art. Therefore, these modifications or improvements made on the basis of not deviating from the spirit of the present application, all belong to the scope of the present application.

Claims

1. A nucleic acid amplification detection module, characterized by, The nucleic acid amplification detection module comprises a fluorescence detection mechanism (1) and a temperature adjusting mechanism (2) which are oppositely and spaced apart along a first direction, a carrier (100) for carrying a reaction sample can be pressed against one side of the temperature adjusting mechanism (2) facing the fluorescence detection mechanism (1), the fluorescence detection mechanism (1) is used for detecting the reaction sample, and the temperature adjusting mechanism (2) is used for heating and cooling the reaction sample. The nucleic acid amplification detection module further comprises a pressing device (5) which can be in a pressing state for pressing the carrier (100) against the temperature adjusting mechanism (2) or in a releasing state for releasing the carrier (100). The pressing device (5) comprises a linear mechanism and a pressing mechanism, the linear mechanism is used for driving the pressing mechanism to translate so as to press or release the carrier (100). The linear mechanism is a cam mechanism (51), a gear mechanism (52) or a crank slider mechanism (53). The nucleic acid amplification detection module further comprises an insertion block (9) arranged on the side of the temperature adjusting mechanism (2) facing the fluorescence detection mechanism (1), the insertion block (9) is provided with an insertion slot (91) for inserting the carrier (100). The nucleic acid amplification detection module further comprises a pressing mechanism (6) for pressing a pressing cavity (101) on the carrier (100), and the pressing mechanism (6) is connected to the temperature adjusting mechanism (2).

2. The nucleic acid amplification detection module of claim 1, wherein, The fluorescence detection mechanism (1) or the temperature adjusting mechanism (2) is arranged in the pressing device (5).

3. The nucleic acid amplification detection module of claim 1, wherein, The nucleic acid amplification detection module further comprises a shell (3), the pressing device (5), the fluorescence detection mechanism (1) and the temperature adjusting mechanism (2) are arranged in the shell (3), and the shell (3) is provided with an opening, and the carrier (100) enters or exits the shell (3) through the opening.

4. The nucleic acid amplification detection module of claim 3, wherein, The cam mechanism (51) comprises a cam (511) and a first guide rod (512), the cam (511) is rotationally connected to the shell (3), the first guide rod (512) is connected to the shell (3) and extends along a first direction, the pressing mechanism is a first pressing mechanism (54), the first pressing mechanism (54) is slidingly connected to the first guide rod (512), and the cam (511) abuts against the first pressing mechanism (54).

5. The nucleic acid amplification detection module of claim 4, wherein, The cam (511) has a peripheral surface (5112) abutting against the first pressing mechanism (54), and the peripheral surface (5112) gradually moves away from a rotation center of the cam (511) from one end to the other end in the peripheral direction.

6. The nucleic acid amplification detection module of claim 4, wherein, The cam mechanism (51) further comprises a first locking piece (513), the first locking piece (513) is arranged on the first guide rod (512) and abuts against the first pressing mechanism (54), so that the first pressing mechanism (54) always abuts against the cam (511).

7. The nucleic acid amplification detection module of claim 6, wherein, One end of the first guide rod (512) is connected with a first flange (514), the first locking piece (513) comprises a spring, the spring is sleeved on the first guide rod (512), one end of the spring abuts against the first flange (514), and the other end abuts against the first pressing mechanism (54).

8. The nucleic acid amplification detection module of any one of claims 4-7, wherein, The cam (511) is arranged on one side of the fluorescence detection mechanism (1) or the temperature adjusting mechanism (2) in a second direction, and the first direction is perpendicular to the second direction.

9. The nucleic acid amplification detection module of claim 8, wherein, Two cam (511) are arranged in the second direction, the two cam (511) are connected through a first connecting shaft (516), and the two cam (511) respectively abut against the first pressing mechanism (54).

10. The nucleic acid amplification detection module of claim 9, wherein, The fluorescence detection mechanism (1) or the temperature adjusting mechanism (2) is located between the two cam (511).

11. The nucleic acid amplification detection module of any one of claims 4-7, wherein, The first pressing mechanism (54) comprises a pressing frame (541) and a first pressing plate (542), the pressing frame (541) is in sliding connection with the first guide rod (512), and the first pressing plate (542) is in elastic connection with the pressing frame (541).

12. The nucleic acid amplification detection module of claim 11, wherein, The first pressing mechanism (54) further comprises a pressing wheel (543) for abutting against the cam (511), and the pressing wheel (543) is rotationally connected to the pressing frame (541).

13. The nucleic acid amplification detection module of claim 12, wherein, The pressing frame (541) is an annular frame.

14. The nucleic acid amplification detection module of claim 11, wherein, A first buffer (544) is arranged between the pressing frame (541) and the first pressing plate (542).

15. The nucleic acid amplification detection module of claim 14, wherein, The first pressing mechanism (54) further comprises a second guide rod (545), the second guide rod (545) is connected to the pressing frame (541) and extends in the first direction, and the first pressing plate (542) is in sliding connection with the second guide rod (545).

16. The nucleic acid amplification detection module of claim 15, wherein, The first buffer (544) is a spring, the spring is sleeved outside the second guide rod (545) and located between the pressing frame (541) and the first pressing plate (542).

17. The nucleic acid amplification detection module of claim 11, wherein, The fluorescence detection mechanism (1) or the temperature adjusting mechanism (2) is arranged in the pressing frame (541), a first avoiding hole (5421) is formed in the first pressing plate (542), and the fluorescence detection mechanism (1) detects the reaction sample through the first avoiding hole (5421).

18. The nucleic acid amplification detection module of claim 3, wherein, The gear mechanism (52) comprises a driving gear (521), a driven gear (522) in meshing connection with the driving gear (521) and a transmission assembly (523), the driven gear (522) is provided with an arc-shaped guide hole (5211), the pressing mechanism is a second pressing mechanism (55), the arc-shaped guide hole (5211) gradually moves away from the center of the driven gear (522) from one end to the other end, the transmission assembly (523) is in sliding connection with the shell (3) in the first direction, one end of the transmission assembly (523) is arranged in the arc-shaped guide hole (5211), the other end is connected with the second pressing mechanism (55), and the second pressing mechanism (55) is driven to translate.

19. The nucleic acid amplification detection module of claim 18, wherein, The gear mechanism (52) further comprises a second locking piece (524) arranged on the transmission assembly (523) and abutting against the second pressing mechanism (55) so that the transmission assembly (523) always abuts against one side of the inner wall of the arc-shaped guide hole (5211).

20. The nucleic acid amplification detection module of claim 19, wherein, The gear mechanism (52) further comprises a fixed guide (527) fixedly connected to the shell (3), and the transmission assembly (523) is slidably connected to the fixed guide (527) in a first direction.

21. The nucleic acid amplification detection module of claim 20, wherein, The second locking piece (524) comprises a spring sleeved on the transmission assembly (523) and located between the fixed guide (527) and the second pressing mechanism (55).

22. The nucleic acid amplification detection module of any one of claims 18-21, wherein, The second pressing mechanism (55) comprises a second pressing plate (551), the transmission assembly (523) is slidably arranged on the second pressing plate (551), the second pressing plate (551) is used for pressing the carrier (100), and a second avoiding hole (5511) is arranged on the second pressing plate (551), and the fluorescence detection mechanism (1) detects the reaction sample through the second avoiding hole (5511).

23. The nucleic acid amplification detection module of claim 22, wherein, A second buffer (528) is arranged between the gear mechanism (52) and the second pressing mechanism (55).

24. The nucleic acid amplification detection module of claim 23, wherein, The other end of the transmission assembly (523) is connected with a second flange (525), the second buffer (528) is a spring, the spring is sleeved on the transmission assembly (523) and located between the second flange (525) and the second pressing plate (551).

25. The nucleic acid amplification detection module of claim 24, wherein, The second pressing mechanism (55) further comprises a pre-pressing block (552) for pre-pressing the second buffer (528), the pre-pressing block (552) is connected to the second pressing plate (551), the pre-pressing block (552) is provided with an accommodating groove (553), the second buffer (528) and the second flange (525) are arranged in the accommodating groove (553), and the second flange (525) can slide relative to the pre-pressing block (552).

26. The nucleic acid amplification detection module of any one of claims 18-21, wherein, The driven gear (522) and the transmission assembly (523) are arranged on one side of the fluorescence detection mechanism (1) or the temperature adjusting mechanism (2) in a second direction, and the first direction is perpendicular to the second direction.

27. The nucleic acid amplification detection module of claim 26, wherein, Two groups of the transmission assembly (523) are arranged in the second direction, the two groups of the transmission assembly (523) are connected through a second connecting shaft (526), the second connecting shaft (526) is slidably arranged in the arc-shaped guide hole (5211), and the fluorescence detection mechanism (1) or the temperature adjusting mechanism (2) is located between the two groups of the transmission assembly (523).

28. The nucleic acid amplification detection module of claim 3, wherein, The crank slider mechanism (53) comprises a connecting rod (531), a slider (532) and a third guide rod (533), the pressing mechanism is a third pressing mechanism (56), the third guide rod (533) is connected to the shell (3) and extends along a first direction, the slider (532) is sleeved outside the third guide rod (533), one end of the connecting rod (531) is rotatably connected to the slider (532) and drives the slider (532) to slide along the third guide rod (533), and the slider (532) is used for driving the third pressing mechanism (56) to press against the carrier (100).

29. The nucleic acid amplification detection module of claim 28, wherein, The crank slider mechanism (53) further comprises a third locking piece (534), the third locking piece (534) is arranged on the third guide rod (533) and abuts against the third pressing mechanism (56), and the third locking piece (534) drives the third pressing mechanism (56) to move to the side where the slider (532) is located.

30. The nucleic acid amplification detection module of claim 29, wherein, An end of the third guide rod (533) is connected with a third flange (535), the third pressing mechanism (56) comprises a pressing rod (561) which is slidably connected to the third guide rod (533), the third locking piece (534) is a spring which is sleeved on the third guide rod (533) and abuts against the pressing rod (561) and the third flange (535).

31. The nucleic acid amplification detection module of any one of claims 28-30, wherein, The third pressing mechanism (56) further comprises a third pressing plate (562), the third pressing plate (562) is provided with a third avoiding hole (5621), and the fluorescent detection mechanism (1) detects the reaction sample through the third avoiding hole (5621).

32. The nucleic acid amplification detection module of claim 31, wherein, A third buffer (536) is arranged between the slider (532) and the third pressing mechanism (56).

33. The nucleic acid amplification detection module of any one of claims 28-30, wherein, The slider (532) and the third guide rod (533) are arranged on one side of the fluorescent detection mechanism (1) or the temperature adjusting mechanism (2) along a second direction, and the first direction is perpendicular to the second direction.

34. The nucleic acid amplification detection module of claim 33, wherein, Two groups of the slider (532) and the third guide rod (533) are arranged along the second direction, the two groups of the slider (532) are connected through a third connecting shaft, the connecting rod (531) is connected with the third connecting shaft, and the fluorescent detection mechanism (1) or the temperature adjusting mechanism (2) is located between the two groups of the third guide rod (533).

35. The nucleic acid amplification detection module of any one of claims 3-7, 18-21, 28-30, wherein, The nucleic acid amplification detection module further comprises a driving piece, the driving piece is connected with the pressing device (5) and drives the pressing device (5) to switch between the pressing state and the loosening state.

36. The nucleic acid amplification detection module of claim 35, wherein, The driving piece is a cover body (4), and the cover body (4) can be in an opening state of opening the opening or a closing state of closing the opening.

37. The nucleic acid amplification detection module of claim 36, wherein, The cover body (4) is switched from the opening state to the closing state, which can drive the pressing device (5) to switch from the loosening state to the pressing state; and the cover body (4) is switched from the closing state to the opening state, which can drive the pressing device (5) to switch from the pressing state to the loosening state.

38. The nucleic acid amplification detection module of claim 3, wherein, The pressing device (5) further comprises a pressing detection mechanism (67) for detecting whether the pressing mechanism presses the carrier (100).

39. The nucleic acid amplification detection module of claim 38, wherein, The pressing detection mechanism (67) comprises a photoelectric switch (671) and a blocking piece (672), one of which is connected to the pressing mechanism, and the other is fixedly connected to the shell (3), the pressing mechanism is in translation, and the blocking piece (672) can enter and exit between the emitting part and the receiving part of the photoelectric switch (671).

40. The nucleic acid amplification detection module of any one of claims 1-7, 18-21, 28-30, wherein, A flat-structure carrier (100) is inserted between the fluorescence detection mechanism (1) and the temperature adjusting mechanism (2).

41. The nucleic acid amplification detection module of any one of claims 7, 18-21, 28-30, wherein, The temperature adjusting mechanism (2) comprises a heating sheet (23) for heating the carrier (100) and a cooler (22) for cooling the heating sheet (23), and the heating sheet (23) is arranged on one side of the cooler (22) along a first direction.

42. The nucleic acid amplification detection module of claim 1, wherein, The nucleic acid amplification detection module further comprises an in-place detection mechanism (7) for detecting whether the carrier (100) is inserted into the slot (91).

43. The nucleic acid amplification detection module of claim 42, wherein, The in-place detection mechanism (7) comprises a travel switch (71) connected to the insertion block (9), and the carrier (100) inserted into the slot (91) can press the travel switch (71).

44. The nucleic acid amplification detection module of claim 43, wherein, The in-place detection mechanism (7) further comprises a pressing sheet (72) rotatably connected to the insertion block (9), and the carrier (100) inserted into the slot (91) can press the pressing sheet (72) to press the travel switch (71).

45. The nucleic acid amplification detection module of claim 1, wherein, The nucleic acid amplification detection module further comprises a to-position detection mechanism (8) for detecting whether the carrier (100) is inserted into a preset position.

46. The nucleic acid amplification detection module of claim 45, wherein, The to-position detection mechanism (8) comprises a to-position protrusion (81) elastically connected to the insertion block (9), and a to-position notch (102) is formed on the carrier (100), and the to-position protrusion (81) can enter and exit the to-position notch (102).

47. The nucleic acid amplification detection module of claim 1, wherein, The pressing mechanism (6) comprises a pressing fixed block, a pressing head (61) and a pressing elastic member (62), the pressing fixed block is connected to the temperature adjusting mechanism (2) and is provided with a stepped hole (213), the pressing head (61) is partially arranged in the stepped hole (213) and can abut against the stepped surface of the stepped hole (213), and the other part of the pressing head (61) extends out of the stepped hole (213), and the pressing elastic member (62) is used for adjusting the length of the pressing head (61) extending out of the stepped hole (213).

48. The nucleic acid amplification detection module of claim 41, wherein, The cooler (22) comprises a cooling main body (221) and a base (223), the cooling main body (221) can circulate cooling liquid to cool the heating sheet (23), and the base (223) is provided with a mounting groove (2231), and the cooling main body (221) is mounted in the mounting groove (2231).

49. A nucleic acid amplification detection all-in-one machine, characterized in that, The nucleic acid amplification detection module (10) comprises any one of claims 1-48. The nucleic acid amplification detection module (10) comprises any one of claims 1-48.

50. The integrated nucleic acid amplification and detection machine of claim 49, wherein, The nucleic acid amplification detection all-in-one machine comprises one or more sets of the nucleic acid amplification detection module (10).

51. The integrated nucleic acid amplification and detection machine of claim 49, wherein, The nucleic acid amplification detection all-in-one machine further comprises a water chiller (20) configured to provide a cooling medium for the temperature adjusting mechanism (2).

52. The integrated nucleic acid amplification and detection machine of claim 51, wherein, The nucleic acid amplification detection all-in-one machine further comprises a control module (30) electrically connected with the water chiller (20) and the nucleic acid amplification detection module (10).

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