A method, device and equipment for nucleic acid synthesis based on enzymatic technology

Abstract

The present invention provides a method, device and electronic device based on enzymatic nucleic acid synthesis. The method based on enzymatic nucleic acid synthesis includes: analyzing the sequence of target DNA; calculating the generation process of the sequence of target DNA according to the sequence of target DNA; nucleic acid reagent, mixing the nucleic acid reagent and enzyme; according to the generation process of the sequence of target DNA, the nucleic acid reagent completes the nucleic acid synthesis reaction. The above method and device provided by the present invention have a simple structure, and can integrate a complex double-stranded nucleic acid synthesis process into one device, and the double-stranded nucleic acid synthesis process has high precision and high efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid synthesis, and in particular to a method, device and equipment for nucleic acid synthesis based on enzymatic technology. Background Art

[0002] Unlike traditional biology, which studies the internal structure of living organisms by dissecting them, synthetic biology has a completely opposite research direction. It starts from the most basic elements and builds components step by step. Among the nucleic acid synthesis technologies in the field of synthetic biology technology, enzymatic nucleic acid synthesis technology is a relatively new synthesis process, which has many advantages over traditional chemical synthesis technology.

[0003] At present, there are multiple technical branches of enzymatic nucleic acid synthesis technology, among which the main technical path is to synthesize single-stranded DNA (deoxyribonucleic acid, DeoxyriboNucleic Acid, abbreviated as DNA) and then amplify it into double-stranded DNA. However, this path has certain defects, among which the most criticized are the limited nucleic acid synthesis length and high error rate. In the process of synthesizing double-stranded DNA from single-stranded DNA bases, due to the instability of the single-stranded DNA bases themselves, the length of the single-stranded DNA bases is limited, and the single-stranded DNA bases themselves are prone to misalignment and errors. As a result, errors in double-stranded DNA are also generated, and the length after synthesis is limited.

[0004] Another path is double-stranded DNA synthesis technology, which uses several double-stranded DNA bases for synthesis. Since the double-stranded DNA used for synthesis is stable in itself, errors are less likely to occur during the continuous synthesis process. At the same time, the length of the synthesized double-stranded DNA can also be greatly extended.

[0005] However, double-stranded DNA synthesis technology has technical barriers such as requiring high throughput, high purity, high complexity and randomness, as well as the need for many consumables, high synthesis costs and the inability to carry out large-scale synthesis. Summary of the invention

[0006] In view of the above technical problems in the prior art, the present invention provides a method for synthesizing nucleic acid based on enzymatic technology, comprising:

[0007] Analyze the sequence of the target DNA;

[0008] Calculate the generation process of the target DNA sequence according to the target DNA sequence;

[0009] Preparing a nucleic acid reagent, and mixing the nucleic acid reagent and an enzyme;

[0010] The nucleic acid reagent is synthesized according to the generation process of the target DNA sequence.

[0011] Furthermore, the step of calculating the generation process of the target DNA sequence according to the target DNA sequence includes:

[0012] Initializing data to identify the sequence of the target DNA;

[0013] According to the sequence of the target DNA, construct a superimposed image of the target DNA after synthesis;

[0014] The overlapping images are divided into a plurality of target DNA fragments, wherein the target DNA fragments contain corresponding sequences of the target DNA.

[0015] Furthermore, the step of synthesizing the nucleic acid reagent according to the generation process of the target DNA sequence includes:

[0016] Creating a dynamic synthesis table, wherein the synthesis table includes information of the target DNA fragment and information of the nucleic acid reagent;

[0017] Identify information of the target DNA fragment and information of the nucleic acid reagent;

[0018] When the information of the nucleic acid reagent is the same as the information of the target DNA fragment, synthesizing the nucleic acid reagent;

[0019] When the information of the nucleic acid reagent is different from the information of the target DNA fragment, the search for the next nucleic acid reagent continues.

[0020] Furthermore, the step of constructing a superimposed image of the target DNA after synthesis according to the sequence of the target DNA comprises:

[0021] Create a vector defined as:

[0022] ;

[0023] Among them, the The overlapping images, the represents the target DNA fragment, the represents nucleic acid reagent; the vector Defined as:

[0024] ;

[0025] Among them, the and , respectively represent the two DNA chains constituting the nucleic acid reagent.

[0026] Furthermore, the step of creating a dynamic synthesis table including the position of the target DNA fragment and the position of the nucleic acid reagent comprises:

[0027] Define the synthesis table as DP, the DP= ;

[0028] Said represents the information of the target DNA fragment, the Information indicating the nucleic acid reagent;

[0029] Regarding the and stated Do the following operations:

[0030] Identify the information of the nucleic acid reagent, record the information of the nucleic acid reagent, and define it as ;

[0031] Identify the information of the target DNA fragment, record the information of the target DNA fragment, and define it as ;

[0032] Judge the and stated Are they the same?

[0033] It should be noted that when the position of the nucleic acid reagent is the same as the position of the target DNA fragment, it means that the content in the target DNA fragment is the same as the content of the nucleic acid reagent.

[0034] Therefore, when the positions of the above two are the same, it means that the arrangement order of the two is consistent and the DNA information contained is also consistent.

[0035] It should be noted that the and stated The range is from 1 to .

[0036] When from 1 to Complete the above Regarding the and stated After the following operation steps are performed, it is defined as the completion of the primary synthesis, and the same operation is then performed on other DNA fragments until the target DNA sequence is synthesized.

[0037] The present invention also provides a device for nucleic acid synthesis based on enzymatic technology, comprising:

[0038] An analytical computing module for analyzing the sequence of the target DNA;

[0039] A DNA template allocation module, used for calculating a generation process of the target DNA sequence according to the target DNA sequence;

[0040] A consumable reagent management module, used for pre-preparing nucleic acid reagents and mixing the nucleic acid reagents with enzymes;

[0041] The DNA synthesis module is used to synthesize the nucleic acid reagent according to the generation process of the target DNA sequence.

[0042] It should be noted that the consumable reagent management module is used to provide nucleic acid reagents and to preserve and store the nucleic acid reagents.

[0043] It should be noted that the consumable reagent management module includes:

[0044] Consumables moving unit and consumables storage management unit;

[0045] The consumables moving unit is arranged on one side of the consumables storage management unit, and the two belong to different workstations;

[0046] The consumable moving unit includes a well plate and a gun head, and the gun head is installed on the well plate. After the gun head collects the nucleic acid reagent, the nucleic acid reagent is moved to the consumable storage management unit.

[0047] The consumable storage management unit freezes and refrigerates the nucleic acid reagent, and provides constant environmental parameters to store the nucleic acid reagent; if it is needed later, the well plate and the gun head in the consumable mobile unit are used to transport and recover the nucleic acid reagent.

[0048] It should be noted that the consumables moving unit and the consumables storage management unit and their coordinated use are described in detail as follows:

[0049] The operator pre-stores the nucleic acid reagent in the consumable storage management unit. The nucleic acid reagent can be stored for a long time in the cold storage module in this block. The system records the inventory of the nucleic acid reagent and prompts the operator to update it immediately;

[0050] The operator determines whether to put the required new nucleic acid reagent into the nucleic acid reagent storage management unit based on the inventory.

[0051] It should be noted that the consumable moving unit is used to move the nucleic acid reagent from the consumable storage management unit to the DNA template distribution module.

[0052] Furthermore, the DNA template distribution module includes:

[0053] An initialization unit, used to initialize data and then identify the sequence of the target DNA;

[0054] A coincidence image construction unit is used to construct a coincidence image of the target DNA after synthesis according to the sequence of the target DNA;

[0055] The segment division unit is used to divide the overlapping images into a plurality of target DNA segments, wherein the target DNA segments contain the corresponding sequences of the target DNA.

[0056] Furthermore, the unit for constructing overlapping images runs the following formula:

[0057] Create a vector defined as:

[0058] ;

[0059] Among them, the The overlapping images, the represents the target DNA fragment, the represents nucleic acid reagent; the vector Defined as:

[0060] Among them, the and , respectively represent the two DNA chains constituting the nucleic acid reagent.

[0061] Further, the DNA synthesis module comprises:

[0062] A table creation unit is used to create a dynamic synthesis table, wherein the synthesis table includes information of the target DNA fragment and information of the nucleic acid reagent;

[0063] An identification unit, used to identify the information of the target DNA fragment and the information of the nucleic acid reagent;

[0064] A judgment unit, used to judge:

[0065] When the information of the nucleic acid reagent is the same as the information of the target DNA fragment, synthesizing the nucleic acid reagent;

[0066] When the information of the nucleic acid reagent is different from the information of the target DNA fragment, the search for the next nucleic acid reagent continues.

[0067] Furthermore, the creation table cell runs the following formula:

[0068] Define the synthesis table as DP, the DP= ;

[0069] Said represents the information of the target DNA fragment, the Information indicating the nucleic acid reagent;

[0070] Regarding the and stated Do the following operations:

[0071] Identify the information of the nucleic acid reagent, record the information of the nucleic acid reagent, and define it as ;

[0072] Identify the information of the target DNA fragment, record the information of the target DNA fragment, and define it as ;

[0073] Judge the and stated Are they the same?

[0074] The present invention also provides an electronic device, comprising:

[0075] Memory, used to store computer programs;

[0076] A processor is used to execute the computer program to implement the method of nucleic acid synthesis based on enzymatic technology.

[0077] The present invention provides a method for nucleic acid synthesis based on enzymatic technology, comprising: analyzing the sequence of target DNA; calculating the generation process of the target DNA sequence according to the sequence of the target DNA; prefabricating nucleic acid reagents, mixing nucleic acid reagents and enzymes; synthesizing the nucleic acid reagents according to the generation process of the target DNA sequence. And a nucleic acid synthesis device based on enzymatic technology using the method.

[0078] The present invention overcomes the problem that traditional biological instrument platforms cannot achieve the production requirements of high throughput, high purity, complexity and high randomness in the double-stranded nucleic acid synthesis process. The double-stranded nucleic acid synthesis is divided into three main areas for separate processing, wherein the DNA template distribution module, the consumable reagent management module and the DNA synthesis module cooperate with each other, and each completes the corresponding processing at their respective stations, and finally realizes the synthesis of double-stranded nucleic acid. The above-mentioned method and device provided by the present invention have a simple structure, and can integrate the complex double-stranded nucleic acid synthesis process into one device, and the double-stranded nucleic acid synthesis process has high precision and high efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0079] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative work, among which:

[0080] Figure 1 It is a schematic diagram of the process of nucleic acid synthesis based on enzymatic technology disclosed in the present invention;

[0081] Figure 2 Yes Figure 1 A schematic flow chart of step S2 is disclosed;

[0082] Figure 3 Yes Figure 2 A schematic flow chart of step S4 is disclosed;

[0083] Figure 4 The present invention provides a schematic diagram of a device structure for nucleic acid synthesis based on enzymatic technology;

[0084] Figure 5 Yes Figure 4 A schematic structural diagram of the disclosed DNA template distribution module 02;

[0085] Figure 6 Yes Figure 4 A schematic diagram of the disclosed DNA synthesis module 04;

[0086] Figure 7 The invention provides an electronic device. DETAILED DESCRIPTION

[0087] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0088] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0089] In addition, in the present invention, descriptions such as "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0090] In the present invention, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0091] In addition, the technical solutions between the various embodiments of the present invention can be combined with each other, but it must be based on the fact that ordinary technicians in the field can implement it. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0092] like Figure 1 As shown, Figure 1 Provided is a method for nucleic acid synthesis based on enzymatic technology, comprising:

[0093] S1, analyzing the sequence of the target DNA;

[0094] S2. Calculating a generation process of the target DNA sequence according to the target DNA sequence;

[0095] S3, pre-preparing a nucleic acid reagent, and mixing the nucleic acid reagent and an enzyme;

[0096] S4. Synthesizing the nucleic acid reagent according to the generation process of the target DNA sequence.

[0097] like Figure 2 As shown, Figure 2 yes Figure 1 The step S2 shown in FIG. 1 comprises:

[0098] S21, initializing data to identify the sequence of the target DNA;

[0099] S22, constructing a superimposed image of the target DNA after synthesis according to the sequence of the target DNA;

[0100] S23, dividing the overlapping images into a plurality of target DNA fragments, wherein the target DNA fragments contain corresponding sequences of the target DNA.

[0101] like Figure 3 As shown, Figure 3 yes Figure 1 The step S4 shown in FIG. 1 comprises:

[0102] The S4 step includes:

[0103] S41, creating a dynamic synthesis table, wherein the synthesis table includes information of the target DNA fragment and information of the nucleic acid reagent;

[0104] S42, identifying the information of the target DNA fragment and the information of the nucleic acid reagent;

[0105] S43, when the information of the nucleic acid reagent is the same as the information of the target DNA fragment, synthesizing the nucleic acid reagent;

[0106] S44: When the information of the nucleic acid reagent is different from the information of the target DNA fragment, continue to search for the next nucleic acid reagent.

[0107] Furthermore, the step of constructing a superimposed image of the target DNA after synthesis according to the sequence of the target DNA comprises:

[0108] Create a vector defined as:

[0109] ;

[0110] Among them, the The overlapping images, the represents the target DNA fragment, the represents nucleic acid reagent; the vector Defined as:

[0111] ;

[0112] Among them, the and , respectively represent the two DNA chains constituting the nucleic acid reagent.

[0113] Furthermore, the step S41 includes:

[0114] Define the synthesis table as DP, the DP= ;

[0115] Said represents the information of the target DNA fragment, the Information indicating the nucleic acid reagent;

[0116] Regarding the and stated Do the following operations:

[0117] Identify the information of the nucleic acid reagent, record the information of the nucleic acid reagent, and define it as ;

[0118] Identify the information of the target DNA fragment, record the information of the target DNA fragment, and define it as ;

[0119] Judge the and stated Are they the same?

[0120] It should be noted that the judgment and stated Do the same steps also include:

[0121] The position of the target DNA fragment is identified, and the position of the nucleic acid reagent is sorted according to the position of the target DNA fragment.

[0122] like Figure 4 As shown, Figure 4 The present invention also provides a device for nucleic acid synthesis based on enzymatic technology, comprising:

[0123] Analysis and calculation module 01, used to analyze the sequence of target DNA;

[0124] A DNA template allocation module 02, used to calculate a generation process of the target DNA sequence according to the target DNA sequence;

[0125] Consumable reagent management module 03, for nucleic acid reagent, mixing the nucleic acid reagent and enzyme;

[0126] The DNA synthesis module 04 is used to synthesize the nucleic acid reagent according to the generation process of the target DNA sequence.

[0127] It should be noted that the DNA synthesis module 04 is mainly used to complete the synthesis process of the nucleic acid reagent. In this process, the reaction is completed to form a longer double-stranded DNA, which is the first step of synthesis.

[0128] During the synthesis process, the nucleic acid reagent needs to be treated at a specific temperature, and then shaken, pipetted, magnetically adsorbed, etc., so that the nucleic acid reagent is fully fused and can be synthesized. After completing the first step of synthesis, the product of the first step of synthesis will be used as raw material to synthesize a more complex second step or even third step DNA sequence. The above operations are repeated continuously to finally form the target DNA sequence.

[0129] It should be noted that the consumable reagent management module is used to provide nucleic acid reagents and to preserve and store the nucleic acid reagents.

[0130] It should be noted that the consumable reagent management module includes:

[0131] Consumables moving unit and consumables storage management unit;

[0132] The consumables moving unit is arranged on one side of the consumables storage management unit, and the two belong to different workstations;

[0133] The consumables moving unit includes a well plate and a gun head, and the gun head is installed on the well plate. After the gun head collects the nucleic acid reagent, the nucleic acid reagent is moved to the consumables storage management unit. The consumables storage management unit freezes and refrigerates the nucleic acid reagent, provides constant environmental parameters, and stores the nucleic acid reagent; if it is needed later, the well plate and the gun head in the consumables moving unit are used to transport and recover the nucleic acid reagent.

[0134] It should be noted that the consumables moving unit and the consumables storage management unit and their coordinated use are described in detail as follows:

[0135] The operator pre-stores the nucleic acid reagent in the consumable storage management unit. The nucleic acid reagent can be stored for a long time in the cold storage module in this block. The system records the inventory of the nucleic acid reagent and prompts the operator to update it immediately;

[0136] The operator determines whether to put the required new nucleic acid reagent into the nucleic acid reagent storage management unit based on the inventory.

[0137] It should be noted that the consumable moving unit is used to move the nucleic acid reagent from the consumable storage management unit to the DNA template distribution module.

[0138] like Figure 5 As shown, the DNA template distribution module 02 includes:

[0139] Initialization unit 021, used to initialize data and then identify the sequence of the target DNA;

[0140] A coincidence image construction unit 022 is used to construct a coincidence image of the target DNA after synthesis according to the sequence of the target DNA;

[0141] The segment division unit 023 is used to divide the overlapped image into a plurality of target DNA segments, wherein the target DNA segments contain the corresponding sequences of the target DNA.

[0142] Furthermore, the unit for constructing the coincident image runs the following formula:

[0143] Create a vector defined as:

[0144] ;

[0145] Among them, the The overlapping images, the represents the target DNA fragment, the represents nucleic acid reagent; the vector Defined as:

[0146] ;

[0147] Among them, the and , respectively represent the two DNA chains constituting the nucleic acid reagent.

[0148] like Figure 6 As shown, the DNA synthesis module 04 includes:

[0149] A table creation unit 041 is used to create a dynamic synthesis table, wherein the synthesis table includes information of the target DNA fragment and information of the nucleic acid reagent;

[0150] An identification unit 042, used to identify the information of the target DNA fragment and the information of the nucleic acid reagent;

[0151] The judging unit 043 is used to judge:

[0152] When the information of the nucleic acid reagent is the same as the information of the target DNA fragment, synthesizing the nucleic acid reagent;

[0153] When the information of the nucleic acid reagent is different from the information of the target DNA fragment, the search for the next nucleic acid reagent continues.

[0154] Furthermore, the creation table cell runs the following formula:

[0155] Define the synthesis table as DP, the DP= ;

[0156] Said represents the information of the target DNA fragment, the Information indicating the nucleic acid reagent;

[0157] Regarding the and stated Do the following operations:

[0158] Identify the information of the nucleic acid reagent, record the information of the nucleic acid reagent, and define it as ;

[0159] Identify the information of the target DNA fragment, record the information of the target DNA fragment, and define it as ;

[0160] Judge the and stated Are they the same?

[0161] like Figure 7 As shown, Figure 7 The structure diagram of an electronic device 60 provided in an embodiment of the present application. The electronic device 60 may specifically include: at least one processor 61, at least one memory 62, a power supply 63, a communication interface 64, an input / output interface 65, and a communication bus 66. The memory 62 is used to store a computer program, which is loaded and executed by the processor 21 to implement the relevant steps in the method for nucleic acid synthesis based on enzymatic technology disclosed in any of the aforementioned embodiments.

[0162] In this embodiment, the power supply 63 is used to provide working voltage for each hardware device on the electronic device 60; the communication interface 64 can create a data transmission channel between the electronic device 60 and the external device, and the communication protocol it follows is any communication protocol that can be applied to the technical solution of the present application, and is not specifically limited here; the input and output interface 65 is used to obtain external input data or output data to the outside world, and its specific interface type can be selected according to specific application needs and is not specifically limited here.

[0163] In addition, the memory 62, as a carrier for storing resources, can be a read-only memory, a random access memory, a disk or an optical disk, etc. The resources stored thereon include an operating system 621, a computer program 622, and data 623 including high-bit video, etc. The storage method can be temporary storage or permanent storage.

[0164] The operating system 621 is used to manage and control the hardware devices and computer programs 622 on the electronic device 60, so as to realize the operation and processing of the massive data 623 in the memory 62 by the processor 61, and it can be Windows Server, Netware, Unix, Linux, etc. In addition to including a computer program that can be used to complete the nucleic acid synthesis method based on enzymatic technology disclosed in any of the aforementioned embodiments, the computer program 622 can further include a computer program that can be used to complete other specific tasks.

[0165] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part.

[0166] The steps of the method or algorithm described in conjunction with the embodiments disclosed herein may be implemented directly using hardware, a software module executed by a processor, or a combination of the two. The software module may be placed in a random access memory (RAM), a memory, a read-only memory (ROM), an electrically programmable ROM, an electrically erasable programmable ROM, a register, a hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art.

[0167] Finally, it should be noted that, in this article, the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of more restrictions, an element defined by the sentence "comprises a ..." does not exclude the presence of other identical elements in the process, method, article or device including the element.

[0168] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A method for nucleic acid synthesis based on enzymatic technology, characterized in that: include: Analyze the sequence of the target DNA; Calculate the generation process of the target DNA sequence according to the target DNA sequence; Preparing a nucleic acid reagent, and mixing the nucleic acid reagent and an enzyme; Synthesizing the nucleic acid reagent according to the generation process of the target DNA sequence; The step of calculating the generation process of the target DNA sequence according to the target DNA sequence comprises: Initializing data to identify the sequence of the target DNA; According to the sequence of the target DNA, construct a superimposed image of the target DNA after synthesis; Dividing the overlapping images into a plurality of target DNA fragments, wherein the target DNA fragments contain corresponding sequences of the target DNA; The step of synthesizing the nucleic acid reagent according to the generation process of the target DNA sequence includes: Creating a dynamic synthesis table, wherein the synthesis table includes information of the target DNA fragment and information of the nucleic acid reagent; Identify information of the target DNA fragment and information of the nucleic acid reagent; When the information of the nucleic acid reagent is the same as the information of the target DNA fragment, synthesizing the nucleic acid reagent; When the information of the nucleic acid reagent is different from the information of the target DNA fragment, continue to search for the next nucleic acid reagent; The step of constructing a superimposed image of the target DNA after synthesis according to the sequence of the target DNA comprises: Create a vector defined as: G=(V,E) Wherein, the G represents the overlapped image, the V represents the target DNA fragment, and the E represents the nucleic acid reagent; the vector E is defined as: E=(u,v) Wherein, the u and v respectively represent the two DNA chains constituting the nucleic acid reagent; The step of creating a dynamic synthesis table, wherein the synthesis table includes information of the target DNA fragment and information of the nucleic acid reagent, comprises: Define the synthesis table as DP, wherein DP=ζ[i, j]; The i represents the information of the target DNA fragment, and the j represents the information of the nucleic acid reagent; ζ performs the following operation on the i and j: Identify the information of the nucleic acid reagent, record the information of the nucleic acid reagent, and define it as j; Identify the information of the target DNA fragment and record the information of the target DNA fragment, which is defined as i; Determine whether the j is the same as the i.

2. A device for nucleic acid synthesis based on enzymatic technology, characterized in that: include: An analytical computing module for analyzing the sequence of the target DNA; A DNA template allocation module, used for calculating a generation process of the target DNA sequence according to the target DNA sequence; A consumable reagent management module, used for pre-preparing nucleic acid reagents and mixing the nucleic acid reagents with enzymes; A DNA synthesis module, used to synthesize the nucleic acid reagent according to the generation process of the target DNA sequence; The DNA template distribution module comprises: An initialization unit, used to initialize data and then identify the sequence of the target DNA; A coincidence image construction unit is used to construct a coincidence image of the target DNA after synthesis according to the sequence of the target DNA; A segmentation unit, used for dividing the overlapping images into a plurality of target DNA segments, wherein the target DNA segments contain corresponding sequences of the target DNA; The DNA synthesis module comprises: A table creation unit is used to create a dynamic synthesis table, wherein the synthesis table includes information of the target DNA fragment and information of the nucleic acid reagent; An identification unit, used to identify the information of the target DNA fragment and the information of the nucleic acid reagent; A judgment unit, used to judge: When the information of the nucleic acid reagent is the same as the information of the target DNA fragment, synthesizing the nucleic acid reagent; When the information of the nucleic acid reagent is different from the information of the target DNA fragment, continue to search for the next nucleic acid reagent; The create table cell runs the following formula: Define the synthesis table as DP, wherein DP=ζ[i, j]; The i represents the information of the target DNA fragment, and the j represents the information of the nucleic acid reagent; ζ performs the following operation on the i and j: Identify the information of the nucleic acid reagent, record the information of the nucleic acid reagent, and define it as j; Identify the information of the target DNA fragment and record the information of the target DNA fragment, which is defined as i; Determine whether the j is the same as the i.

3. An electronic device, characterized in that: include: Memory, used to store computer programs; A processor, configured to execute the computer program to implement the method for nucleic acid synthesis based on enzymatic technology as claimed in claim 1.

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