A method for processing nasal secretion samples

Abstract

The present invention relates to the technical field of medical devices, and in particular to a method for processing nasal secretion samples. Since nasal secretions are collected through cotton pads, the cotton pads with nasal secretions are blown away by circulating wind to remove aerosols and moisture in the nasal secretions, the aerosols are filtered and absorbed by a filtering unit, and the moisture is absorbed by a drying unit to filter and air-dry the cotton pads with nasal secretions, remove mucin on the cotton pads after filtration and air-drying, place the cotton pads in a container and inject diluent to dilute, separate the cotton pads and the liquid with nasal secretions in the diluted cotton pads, so as to obtain detectable secretions with nasal secretions, and then through this processing method, aerosols and mucin in nasal secretions are effectively removed, and the accuracy of subsequent nasal secretion detection results is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a method for processing nasal secretion samples. Background Art

[0002] At present, the method of obtaining nasal secretions to diagnose allergic rhinitis or local allergic rhinitis is safer and non-invasive than the method of obtaining peripheral blood, which can improve the accuracy of diagnosis and provide patients with earlier, accurate and targeted individualized treatment. This method can also be combined with the evaluation of the expression level of secretory proteins in nasal secretions, and then the two different inflammatory carriers of nasal secretions, namely, allergen-specific IgE and inflammatory factors, can be used to jointly diagnose allergic rhinitis, which is truly safe, efficient, economical and non-invasive, and more in line with the concept of today's precision medicine.

[0003] In the prior art, the commonly used sampling body for specific allergen detection of nasal secretions is to collect samples from the patient's nasal cavity with an expanded sponge, place it in a centrifuge tube filled with physiological saline for soaking, then take out the expanded sponge and place it in a container with a filter tip, then put it into a centrifuge tube for centrifugation, so as to separate the solid and liquid to obtain liquid nasal secretions. However, this method takes a long time to process and is complicated to operate. At the same time, a large amount of mucin is mixed in the secretions, which will affect the clinical test results. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the above-mentioned shortcomings and deficiencies of the prior art, the present invention provides a method for processing nasal secretion samples, which solves the technical problem of inaccurate nasal secretion detection results.

[0006] (II) Technical solution

[0007] In order to achieve the above object, the main technical solutions adopted by the present invention include:

[0008] The embodiment of the present invention provides a method for processing a nasal secretion sample, which comprises the following steps:

[0009] S1. Collect nasal secretions through cotton pads;

[0010] S2. The cotton piece with nasal secretions is blown away by circulating wind to remove aerosol and moisture in the nasal secretions, the aerosol is filtered and absorbed by the filtering unit, and the moisture is absorbed by the drying unit, so as to filter and air-dry the cotton piece with nasal secretions;

[0011] S3, after removing the mucin on the cotton sheet after filtering and air drying, the cotton sheet is placed in a container and a diluent is injected for dilution;

[0012] S4. Separate the cotton piece and the liquid containing nasal secretions from the diluted cotton piece in step S3 to obtain detectable secretions.

[0013] Preferably, in step S4, the cotton piece and the liquid containing nasal secretions are separated by a centrifuge.

[0014] Preferably, in step S2, the cotton sheet containing nasal secretions is filtered and air-dried by an air-drying device.

[0015] Preferably, the air-drying device comprises a box body, a cover body, a fan, a drying unit, a filter unit, a sample box and a refrigeration module; the cover body is buckled on the box body, the box body is recessed to form a cooling space, and the refrigeration module is arranged inside the box body to cool the cooling space; the cooling space is separated by a spacer to form a first space and a second space, the sample box is arranged on the spacer, the filter unit and the drying unit are arranged at the bottom of the first space and the second space respectively, a third space is formed between the filter unit, the drying unit and the box body, and the fan is arranged in the third space; the cotton sheet is placed in the sample box, and the fan blows air in the direction of the filter unit, so that the airflow passes through the filter unit, the sample box and the drying unit in turn and then flows back to the third space to air-dry and filter the cotton sheet in the sample box.

[0016] Preferably, an opening groove is provided on the spacer, and the sample box is opposite to the opening groove and is detachably arranged on the spacer.

[0017] Preferably, the sample box comprises a plurality of rectangular accommodating through holes arranged in an array, and the openings of the rectangular accommodating through holes are horizontally oriented; the cotton sheet is a rectangular sheet structure, and the width of the cotton sheet matches the diagonal length of the rectangular accommodating holes.

[0018] Preferably, it also includes a drying and filtering assembly; the head end of the sample box is clamped with the spacer, and the drying and filtering assembly is clamped with the tail end of the sample box.

[0019] Preferably, the drying and filtering assembly comprises a box body and a filtering group and a drying group sequentially arranged in the box body.

[0020] Preferably, the drying unit is detachably connected to the bottom of the first space; and the filtering unit is detachably connected to the bottom of the second space.

[0021] Preferably, the spacer is a connecting plate, which is vertically oriented in the box and the bottom of the spacer is spaced apart from the bottom of the box; the side walls, cover, connecting plate and drying unit of the box form a first space; the side walls, cover, connecting plate and filtering unit of the box form a second space.

[0022] (III) Beneficial effects

[0023] The beneficial effects of the present invention are:

[0024] The nasal secretion sample processing method of the present invention collects nasal secretions through cotton pads, blows away aerosols and moisture in the nasal secretions through circulating wind, filters and absorbs aerosols through a filtering unit, absorbs moisture through a drying unit, filters and air-dries the cotton pads with nasal secretions, removes mucin on the cotton pads after filtration and air-drying, places the cotton pads in a container and injects a diluent to dilute them, separates the cotton pads and the liquid with nasal secretions in the diluted cotton pads, and obtains detectable secretions with nasal secretions. Furthermore, through this processing method, aerosols and mucin in nasal secretions are effectively removed, thereby improving the accuracy of subsequent nasal secretion detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 is a flow chart of the nasal secretion sample processing method of the present invention;

[0026] Figure 2 It is a structural schematic diagram of the air-drying device in the present invention;

[0027] Figure 3 for Figure 2 A top view of the

[0028] Figure 4 for Figure 2 Schematic diagram of longitudinal section;

[0029] Figure 5 This is a schematic diagram of the structure in which the cotton sheet is installed in the sample box.

[0030] [Description of Reference Numerals]

[0031] 1: Cotton pad;

[0032] 2: air drying device; 21: box body; 22: cover body; 23: fan; 24: drying unit; 25: filtering unit; 26: sample box; 261: rectangular receiving through hole; 27: spacer; 28: drying and filtering assembly;

[0033] 3: First space;

[0034] 4: Second space;

[0035] 5: The third space;

[0036] 6: Storage space. DETAILED DESCRIPTION

[0037] In order to better explain the present invention and facilitate understanding, the present invention is described in detail below through specific implementation modes in conjunction with the accompanying drawings.

[0038] like Figure 1 As shown, this embodiment provides a method for processing nasal secretion samples, which adopts the following steps:

[0039] S1, collect nasal secretions through cotton pad 1;

[0040] S2, placing the cotton piece 1 with nasal secretions into the air drying device 2, blowing away the aerosol and moisture in the nasal secretions through the circulating air in the air drying device 2, filtering and absorbing the aerosol through the filtering unit 25, and absorbing the moisture through the drying unit 24, so as to filter and air dry the cotton piece 1 with nasal secretions;

[0041] S3, after removing the mucin on the cotton sheet 1 after filtering and air drying, the cotton sheet 1 is placed in a container and a diluent is injected for dilution. Since the mucin is a macromolecular substance, it is located on the outside of the cotton sheet 1 after air drying, and the secretion to be detected is a small molecule substance, so it enters the pores of the cotton sheet 1 during collection. The diluent can be one of physiological saline, phosphate buffer or double distilled water;

[0042] S4. Separate the cotton piece 1 and the liquid containing nasal secretions in the diluted cotton piece 1 in step S3 by a centrifuge, take out the dried cotton piece 1 after centrifugation to obtain the liquid for detecting secretions, and then through this treatment method, effectively remove the aerosol and mucin in the nasal secretions, thereby improving the accuracy of subsequent nasal secretion detection results.

[0043] like Figure 2-4 As shown, the air drying device 2 includes a box 21, a cover 22, a fan 23, a drying unit 24, a filter unit 25, a sample box 26 and a refrigeration module (not shown). The cover 22 is buckled on the box 21, and the box 21 is recessed to form a cooling space. The refrigeration module is arranged inside the box 21 to refrigerate the cooling space. The cooling space is separated by a spacer 27 to form a first space 3 and a second space 4. The sample box 26 is arranged on the spacer 27. The filter unit 25 and the drying unit 24 are arranged at the bottom of the first space 3 and the second space 4 respectively. A third space 5 is formed between the filter unit 25, the drying unit 24 and the box 21. The fan 23 is arranged in the third space 5, and the cotton sheet 1 is placed in the sample box 26. It should be noted that the refrigeration module is a prior art that can achieve a refrigeration effect. In this embodiment, the temperature in the cooling space is 4-6°C.

[0044] The fan 23 blows air in the direction of the filter unit 25, and a negative pressure is formed in the third space 5, so that the airflow passes through the filter unit 25, the sample box 26 and the drying unit 24 in sequence and then flows back to the third space 5 to air dry and filter the cotton sheet 1 in the sample box 26. Among them, the filter unit 25 is used to filter and absorb the aerosol in the airflow to avoid the aerosol with viruses remaining in the box 21 to contaminate the next batch of samples, thereby further improving the accuracy of the nasal secretion detection results, and the drying unit 24 is used to absorb the moisture in the airflow.

[0045] The spacer 27 is a connecting plate, which is vertically oriented and arranged in the box body 21, and the bottom of the spacer is spaced from the bottom of the box body 21. The side wall of the box body 21, the cover body 22, the connecting plate and the drying unit 24 form a first space 3, and the side wall of the box body 21, the cover body 22, the connecting plate and the filter unit 25 form a second space 4. To facilitate the installation and disassembly of the drying unit 24 and the filter unit 25, the drying unit 24 is detachably connected to the bottom of the first space 3, and the filter unit 25 is detachably connected to the bottom of the second space 4. Of course, to facilitate the placement of the cotton sheet 1 after air drying, a storage space is also provided on one side of the box body 21, wherein the storage space is an independent space.

[0046] like Figure 5 As shown, to facilitate the placement and removal of samples, the sample box 26 includes a plurality of rectangular receiving through holes 261 arranged in an array, the openings of the rectangular receiving through holes 261 are horizontally oriented, the cotton sheet 1 is a rectangular sheet structure, and the width of the cotton sheet 1 matches the diagonal length of the rectangular receiving hole. To facilitate the installation and removal of the sample box 26, an opening slot is provided on the spacer 27, and the sample box 26 is detachably arranged on the spacer 27 opposite to the opening slot.

[0047] In order to increase the service life of the filter unit 25 and the drying unit 24 and avoid frequent replacement, the air drying device 2 also includes a drying filter assembly 28. The head end of the sample box 26 is clamped with the spacer 27, and the drying filter assembly 28 is clamped with the tail end of the sample box 26. The drying filter assembly 28 includes a box body and a filter group and a drying group sequentially arranged in the box body. Since the drying filter assembly 28 is connected to the tail end of the sample box 26, after the airflow passes through the sample box 26, the aerosol and moisture in the sample are first filtered and dried by the drying filter assembly 28, and then enter the filter unit 25 and the drying unit 24. Therefore, it is only necessary to frequently replace the drying filter assembly 28 with a relatively small volume and a low price. At the same time, since the drying filter assembly 28 is clamped with the sample box 26, it is also convenient for disassembly and installation.

[0048] In the description of the present invention, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0049] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0050] In the present invention, unless otherwise clearly specified and limited, when a first feature is “on” or “below” a second feature, it may be that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, when a first feature is “above”, “above” or “above” a second feature, it may be that the first feature is directly above or obliquely above the second feature, or it may simply mean that the first feature is higher in level than the second feature. When a first feature is “below”, “below” or “below” a second feature, it may be that the first feature is directly below or obliquely below the second feature, or it may simply mean that the first feature is lower in level than the second feature.

[0051] In the description of this specification, the description of the terms "one embodiment", "some embodiments", "embodiment", "example", "specific example" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, unless they are contradictory.

[0052] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may alter, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A method for processing nasal secretion samples, characterized in that: Use the following steps: S1. Collect nasal secretions using cotton pad (1); S2, using circulating air to blow away aerosol and water in the nasal secretions from the cotton sheet (1) carrying the nasal secretions, filtering and absorbing the aerosol through the filtering unit (25), and absorbing the water through the drying unit (24), so as to filter and air-dry the cotton sheet (1) carrying the nasal secretions; In the step S2, the cotton sheet (1) containing nasal secretions is filtered and air-dried by an air-drying device (2); S3, after removing the mucin on the cotton sheet (1) after filtering and air-drying, the cotton sheet (1) is placed in a container and a diluent is injected to dilute it; S4, separating the cotton piece (1) and the liquid containing nasal secretions in the cotton piece (1) diluted in step S3 to obtain detectable secretions; In the step S4, the cotton piece (1) and the liquid containing nasal secretions are separated by a centrifuge; The air drying device (2) comprises a box body (21), a cover body (22), a fan (23), the drying unit (24), the filtering unit (25), a sample box (26) and a refrigeration module; The cover (22) is buckled onto the box (21); the box (21) is recessed to form a cooling space; and the refrigeration module is arranged inside the box (21) to cool the cooling space; The cooling space is separated by a spacer (27) to form a first space (3) and a second space (4); the sample box (26) is arranged on the spacer (27); the filter unit (25) and the drying unit (24) are arranged at the bottom of the first space (3) and the second space (4), respectively; a third space (5) is formed between the filter unit (25), the drying unit (24) and the box body (21); and the fan (23) is arranged in the third space (5); The cotton sheet (1) is placed in the sample box (26), and the fan (23) blows air in the direction of the filter unit (25), so that the airflow passes through the filter unit (25), the sample box (26), and the drying unit (24) in sequence and then flows back to the third space (5) to dry and filter the cotton sheet (1) in the sample box (26).

2. The method for processing nasal secretion samples according to claim 1, characterized in that: The spacer (27) is provided with an opening groove, and the sample box (26) is opposite to the opening groove and is detachably arranged on the spacer (27).

3. The method for processing nasal secretion samples according to claim 1, characterized in that: The sample box (26) comprises a plurality of rectangular accommodating through holes (261) arranged in an array, and the openings of the rectangular accommodating through holes (261) are arranged in a horizontal orientation; The cotton sheet (1) is a rectangular sheet structure, and the width of the cotton sheet (1) matches the diagonal length of the rectangular receiving hole.

4. The method for processing nasal secretion samples according to claim 1, characterized in that: Also included is a dry filter assembly (28); The head end of the sample box (26) is snap-fitted to the spacer (27), and the drying and filtering assembly (28) is snap-fitted to the tail end of the sample box (26).

5. The method for processing nasal secretion samples according to claim 4, characterized in that: The drying and filtering assembly (28) comprises a box body, and a filtering group and a drying group which are sequentially arranged in the box body.

6. The method for processing nasal secretion samples according to claim 4, characterized in that: The drying unit (24) is detachably connected to the bottom of the first space (3); The filter unit (25) is detachably connected to the bottom of the second space (4).

7. The method for processing nasal secretion samples according to claim 1, characterized in that: The spacer (27) is a connecting plate, the connecting plate is vertically oriented and arranged in the box body (21), and the bottom of the connecting plate is spaced apart from the bottom of the box body (21); The side wall of the box body (21), the cover body (22), the connecting plate and the drying unit (24) form the first space (3); The side wall of the box body (21), the cover body (22), the connecting plate and the filter unit (25) form the second space (4).

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