A production device for preparing amino acid biostimulants by enzymatic hydrolysis of marine fish protein

By designing a production device with a driving device for the production of amino acid biostimulants through proteolysis of marine fish, the existing technology problems of difficulty in sampling due to the high temperature of the reactor and difficulty in recovering the protein extract through traditional filtration have been solved, achieving a more efficient process. Protein enzymatic hydrolysis reaction and extraction solution recovery.

CN118792157BActive Publication Date: 2025-01-24QINGDAO HAIDELONG BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411287659.X
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-09-14
Publication Date
2025-01-24
Estimated Expiration
2044-09-14

AI Technical Summary

Technical Problem

The existing fish proteolytic reactor is not convenient to open immediately for sampling when the temperature is too high, and it is difficult for traditional filtration processes to fully recover the protein extract, which increases the production cycle and cost.

Method used

A production device for marine fish protease is designed to prepare amino acid bio -stimulus. The rotation of the fish slurry treatment device and the enzymatic disintegration device is used to realize the enzymatic response of the protein, and the protein extract is used to filter the protein.

Benefits of technology

This device can reduce the transfer of protein extract without increasing the cost of equipment, improve the recycling efficiency of protein extract, and reduce the production cycle and cost.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention relates to the technical field of biostimulant preparation, and specifically, to a production device for preparing amino acid biostimulants by enzymatic hydrolysis of marine fish protein, which uses a driving device to drive a fish paste processing device in a fixed device and a structure in an enzymatic hydrolysis device to rotate, thereby completing the enzymatic hydrolysis reaction of marine fish protein, and then completing the production of amino acid biostimulants. The production device for preparing amino acid biostimulants by enzymatic hydrolysis of marine fish protein can simultaneously drive a centrifugal cylinder and a rotating cover to rotate at the same time through a driving motor, and after starting an electric push rod to move a mobile tank upward, a protein extract can be directly poured into the interior of the mobile tank, which saves costs and reduces the time for transporting the protein extract between two processing equipment in the traditional process.
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Description

Technical Field

[0001] The invention relates to the technical field of biostimulant preparation, in particular to a production device for preparing amino acid biostimulant by enzymatic hydrolysis of marine fish protein. Background Art

[0002] The preparation of amino acid biostimulants by enzymatic hydrolysis of marine fish protein is an innovative biotechnology process. It uses specific enzymatic hydrolysis technology to break down marine fish protein into small molecule amino acids and other biologically active substances. These decomposition products are rich in a variety of amino acids and trace elements that are beneficial to plant growth. As biostimulants, they have broad application potential in the agricultural field.

[0003] The patent with publication number CN220597516U discloses a reactor suitable for fish protein hydrolysis, including a sampling port, a sampling tube is fixedly installed on the inner wall surface of the sampling port, a fixing plate is fixedly installed on the outer wall surface of the reactor body, a movable groove is opened on one side surface of the fixing plate, and a sliding rod is fixedly installed between the inner walls on both sides of the movable groove. When the existing fish protein hydrolysis reactor is in use, it is not convenient to open the reactor immediately for sampling and observation due to the high internal temperature of the device. The sampling can only be carried out after the internal temperature drops, which makes the whole sampling process time-consuming and laborious.

[0004] In the traditional fish protein extraction process, the mixture after crushing often contains insoluble impurities such as fish bone fragments and fibers. During filtration, these impurities are retained on the filter medium, while the protein extract flows out. However, this process has limitations: first, after filtration, extra time is required to disassemble the equipment, clean the medium and rinse the reactor, which increases the production cycle and cost; second, due to the water absorption of fish bone fragments and fibers, traditional filtration methods are difficult to fully recover the protein extract; finally, protein extraction and enzymatic hydrolysis reactions are usually carried out in two groups of reactors, and the extract needs to be transported through pipes or other automated devices. Although this reduces the risk of contamination, it also increases the equipment investment cost.

[0005] In view of this, we propose a production device for preparing amino acid biostimulants by enzymatic hydrolysis of marine fish protein. Summary of the invention

[0006] The object of the present invention is to provide a production device for preparing amino acid biostimulants by enzymatic hydrolysis of marine fish proteins, so as to solve the problems raised in the above-mentioned background technology.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A production device for preparing amino acid biostimulants by enzymatic hydrolysis of marine fish protein, wherein when in use, a driving device is used to drive a fish paste processing device in a fixed device and a structure in an enzymatic hydrolysis device to rotate, thereby completing the enzymatic hydrolysis reaction of marine fish protein, and further completing the production of amino acid biostimulants;

[0009] The fixing device comprises a protein extraction tank, a jacket tank body arranged outside the bottom of the protein extraction tank, and a placement portion at the bottom surface of the protein extraction tank;

[0010] The placement part includes a placement table, a fixed pipe arranged at the center of the bottom surface of the placement table, a liquid blocking cover slidably connected to the inside of the fixed pipe, an upper lifting rod arranged at the center of the top surface inside the liquid blocking cover, and a jacket spring sleeved on the outside of the upper lifting rod;

[0011] The fish paste processing device comprises a centrifugal cylinder with a plurality of openings on the outer wall, a cylinder wall filter screen arranged on the inner side of the openings of the centrifugal cylinder, a plurality of mixing rods regularly arranged on the inner wall of the centrifugal cylinder, a cylinder wall ring tooth sleeved on the outer wall of the centrifugal cylinder near the top position, a plurality of limit blocks regularly arranged on the inner wall of the centrifugal cylinder near the bottom position, a downward pressure spring used to provide downward pressure for the plurality of limit blocks, and a placing plate arranged at the bottom opening of the centrifugal cylinder.

[0012] In the technical solution of the present invention, the enzymatic hydrolysis device includes a rotating cover body, a cover wall ring gear sleeved on the outer side wall of the rotating cover body, a stirring rod arranged on the inner top surface of the rotating cover body, a ball valve arranged on the outer side of the pipe opening at the bottom of the rotating cover body, a movable tank body sleeved on the inner side of the rotating cover body, an upward lifting pipe arranged at the center position of the top surface of the movable tank body, and an electric push rod used to drive the movable tank body to move up and down;

[0013] The driving device comprises a driving motor, a driving gear sleeved on the outside of the output shaft of the driving motor, and a docking gear that rotates as the driving gear rotates;

[0014] A fixing ring body is integrally formed at a position near the top of the inner wall of the protein extraction tank, the outer shell tank body is welded and fixed to the outer side of the protein extraction tank, and convex bin bodies are integrally formed on the outer side walls at the left and right ends of the outer shell tank body.

[0015] In the technical solution of the present invention, a connecting box is integrally formed on the front outer side walls of the protein extraction tank and the outer shell tank body, a plurality of support frames are welded and fixed on the outer side wall of the outer shell tank body, and a sealing cover is rotatably connected to the outer side of the top opening of the protein extraction tank through a hinge.

[0016] In the technical solution of the present invention, a limiting groove is provided at the top surface of the placing table, the fixed tube is integrally formed with the placing table and is fixed on the inner bottom surface of the protein extraction tank by clamping, the through grooves provided on the outer tube wall of the fixed tube are internally connected with tube wall filters by screws, the lifting rod is welded and fixed to the inner top surface of the liquid-blocking cover, and the upper and lower ends of the outer sleeve spring are respectively welded and fixed to the bottom surface of the placing table and the inner top surface of the liquid-blocking cover.

[0017] In the technical solution of the present invention, the centrifugal cylinder is rotatably connected to the top surface of the placement table, the cylinder wall filter is fixedly connected to the open inner wall of the centrifugal cylinder by screws, and the mixing rod is clamped and fixed to the inner wall of the centrifugal cylinder.

[0018] In the technical solution of the present invention, the cylinder wall ring tooth is fixedly connected to the outer wall of the cylinder wall ring tooth by a bayonet pin, the bottom horizontal height of the cylinder wall ring tooth is higher than the top horizontal height of the fixed ring body, the limit block is slidably connected to the groove on the bottom surface of the inner convex ring on the inner wall of the centrifugal cylinder body near the bottom surface, and the upper and lower ends of the downward pressure spring are respectively welded and fixed to the top surface of the groove wall of the groove on the bottom surface of the convex ring in the centrifugal cylinder body and the bottom surface of the limit block.

[0019] In the technical solution of the present invention, the supporting plate is slidably connected to the bottom opening of the centrifugal cylinder body, the bottom surface of the upper ring plate of the supporting plate is in conflict with the top surface of the convex ring in the centrifugal cylinder body, and the top surface of the lower ring plate of the supporting plate is provided with a plurality of plate surface grooves which are the same in number and correspond in position to the limit blocks, and a plate bottom plug block which is adapted to the size of the limit groove is integrally formed at the center position of the bottom surface of the supporting plate.

[0020] In the technical solution of the present invention, the rotating cover body is rotatably connected to the bottom opening of the outer shell tank body, the cover wall ring teeth are fixedly connected to the outer wall of the outer shell tank body by a bayonet pin, the stirring rod is clamped and fixed to the inner top surface of the rotating cover body, the spherical valve flange is connected to the pipe mouth of the bottom surface of the rotating cover body, the movable tank body is rotatably connected to the inside of the rotating cover body, the upper lifting tube is integrally formed with the movable tank body, and a plurality of regularly distributed liquid holes that pass through the inside and outside are opened on the outer tube wall of the upper lifting tube, the electric push rod is fixedly connected to the inner bottom surface of the outer convex warehouse body by screws, and the top end of the telescopic rod of the electric push rod is fixedly connected to a connecting plate clamped and fixed to the outer wall of the movable tank body by bolts.

[0021] In the technical solution of the present invention, the driving motor is fixedly connected to the top surface of the connecting box body by bolts, the driving gear is fixedly connected to the output shaft of the driving motor by a bayonet pin, the driving gear is meshed with the cylinder wall ring teeth, the docking gear is rotatably connected to the inner bottom surface of the connecting box body and meshed with the cover wall ring teeth, and the outer sides of the driving gear and the docking gear are provided with a rotating shaft whose upper and lower ends are rotatably connected to the inside of the connecting box body, and the upper and lower ends of the rotating shaft are both clamped and fixed with shaft end gears that mesh with the driving gear and the docking gear respectively.

[0022] In the technical solution of the present invention, the use includes the following steps:

[0023] S1. First, marine fish are selected as raw materials, cleaned, scaled, and eviscerated, and then mechanically crushed into fish paste;

[0024] S2, then, open the sealing cover in the fixing device, put the processed fish paste into the inside of the centrifugal cylinder in the fish paste processing device, and pour saline solution into the protein extraction tank;

[0025] S3, after the pH value of the salt solution is adjusted to acidic by dilute hydrochloric acid, the sealing cover is closed again, and the driving motor in the driving device is started to drive the driving gear to rotate. After the driving gear contacts the cylinder wall ring gear, the centrifugal cylinder as a whole is driven to rotate. After the mixing rod fully stirs and mixes the fish paste and the salt solution, the protein in the fish paste is dissolved in the salt solution;

[0026] S4. Subsequently, the electric push rod in the enzymatic hydrolysis device is started to drive the movable tank body to move upward as a whole through the connecting plate, thereby driving the lifting tube to move upward together;

[0027] S5, the upward lifting tube squeezes the liquid blocking cover in the placement part, and drives the liquid blocking cover to move upward inside the fixed tube. At this time, the protein extract passes through the tube wall filter and the liquid hole and then flows into the interior of the movable tank;

[0028] S6. As the liquid blocking cover moves, the lifting rod moves upward with it, driving the position of the placing plate to move upward, and the plate surface slot on the placing plate immediately conflicts with the bottom surface of the inner convex ring used to place the limit block in the centrifugal cylinder. As the centrifugal cylinder continues to rotate, the limit block is pushed into the inside of the plate surface slot under the action of the downward pressure spring, driving the placing plate to rotate as a whole;

[0029] S7. At this time, the output power of the driving motor is increased to drive the centrifugal cylinder and the placing plate to rotate at a high speed, so that the protein extract retained in the centrifugal cylinder is filtered by centrifugation and flows into the interior of the mobile tank;

[0030] S8, after the electric push rod is controlled to restore the position of the movable tank, as the driving motor is started, the rotating cover body that is rotating will mix the protein extract and the neutral protease placed in the movable tank in advance through the internal stirring rod, so that the protein in the extract is enzymatically hydrolyzed into amino acids and small peptides through the enzymatic reaction;

[0031] S9, after the enzymatic hydrolysis reaction is completed, the driving motor is turned off, and the ball valve is opened to discharge the enzymatic hydrolysis liquid from the interior of the mobile tank;

[0032] S10. Subsequently, the enzymatic hydrolysate is subjected to ultrafiltration membrane separation technology to further purify the amino acids and small peptide products, and after removing the macromolecular impurities and salts, the purified products are concentrated and dried to obtain the amino acid biostimulant product prepared by fish protein hydrolysis.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] 1. The production device for preparing amino acid biostimulants by protein hydrolysis of marine fish protein can drive the centrifugal cylinder and the rotating cover to rotate at the same time through the driving motor, and after the electric push rod is started to move the mobile tank upward, the protein extract can be directly poured into the interior of the mobile tank, which saves costs and reduces the time for transferring the protein extract between two processing equipment in the traditional process.

[0035] 2. In the production device for preparing amino acid biostimulants by enzymatic hydrolysis of marine fish protein, while the lifting tube drives the liquid blocking cover to change, the lifting rod will also drive the change of the position of the holding plate in the fish paste processing device. At this time, the limit block will extend to the inside of the groove on the plate surface, and after accelerating the output power of the driving motor, the centrifugal cylinder can be rotated at a high speed together with the holding plate, so as to realize centrifugal filtration of the fish paste inside the centrifugal cylinder and fully recover the protein extract.

[0036] 3. In the production device for preparing amino acid biostimulants by protein hydrolysis of marine fish protein, after the centrifugal cylinder completes centrifugal filtration and the position of the movable tank is restored, the operator can replace the entire fish paste processing device by briefly shutting down the drive motor and clean the centrifugal cylinder in the replaced fish paste processing device, which reduces the cleaning of the inner wall of the protein extraction tank and improves the continuity of the overall production process. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0038] Figure 2 It is a schematic cross-sectional view of the overall structure of the present invention;

[0039] Figure 3 It is a schematic cross-sectional view of the structure of the fixing device in the present invention;

[0040] Figure 4 It is a schematic cross-sectional view of the structure of the placement part in the present invention;

[0041] Figure 5 It is a schematic cross-sectional view of the structure of the fish paste processing device of the present invention;

[0042] Figure 6 It is a partial structural cutaway schematic diagram of the fish paste processing device of the present invention;

[0043] Figure 7 For the present invention Figure 6 A magnified schematic diagram of part A;

[0044] Figure 8 It is a schematic cross-sectional view of the structure of the placing plate in the present invention;

[0045] Fig. 9 It is a schematic diagram of the cross-section structure of the enzymatic hydrolysis device of the present invention;

[0046] Fig.10 It is a schematic diagram of the structure of the driving device in the present invention;

[0047] Description of reference numerals:

[0048] 100, fixing device; 110, protein extraction tank; 111, fixing ring body; 120, outer shell tank body; 121, outer convex warehouse body; 130, placement part; 131, placement table; 1310, limiting groove; 132, fixing pipe; 133, pipe wall filter; 134, liquid blocking cover; 135, lifting rod; 136, outer shell spring; 140, connecting box body; 150, support frame; 160, sealing cover;

[0049] 200, fish paste processing device; 210, centrifugal cylinder; 220, cylinder wall filter; 230, mixing rod; 240, cylinder wall ring gear; 250, limit block; 260, downward pressure spring; 270, support plate; 271, plate surface slot; 272, plate bottom plug;

[0050] 300, enzymolysis device; 310, rotating cover; 320, cover wall ring gear; 330, stirring rod; 340, ball valve; 350, movable tank; 360, lifting pipe; 361, liquid hole; 370, electric push rod; 380, connecting plate;

[0051] 400, driving device; 410, driving motor; 420, driving gear; 430, docking gear; 440, rotating shaft; 450, shaft end gear. DETAILED DESCRIPTION

[0052] The following will be combined with the accompanying drawings in the present invention to clearly and completely describe the technical solutions in the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0053] See also Figure 1-Figure 10 As shown, this embodiment provides a technical solution:

[0054] A production device for preparing amino acid biostimulants by enzymatic hydrolysis of marine fish protein, comprising the following steps when used:

[0055] S1. First, marine fish are selected as raw materials, cleaned, scaled, and eviscerated, and then mechanically crushed into fish paste;

[0056] S2, then, open the sealing cover 160 in the fixing device 100, put the processed fish paste into the centrifugal cylinder 210 in the fish paste processing device 200, and pour the salt solution into the protein extraction tank 110;

[0057] S3, after adjusting the pH value of the salt solution to the range of 3.5-5.0 by dilute hydrochloric acid, the sealing cover 160 is closed again, and the driving motor 410 in the driving device 400 is started to drive the driving gear 420 to rotate, and after the driving gear 420 contacts the cylinder wall ring gear 240, the centrifugal cylinder 210 is driven to rotate as a whole, and the mixing rod 230 fully stirs and mixes the fish paste and the salt solution, and the protein in the fish paste is dissolved in the salt solution;

[0058] S4. Subsequently, the electric push rod 370 in the enzymolysis device 300 is started, and the movable tank 350 is driven to move upward as a whole through the connecting plate 380, thereby driving the lifting tube 360 ​​to move upward together;

[0059] S5, the lifting tube 360 ​​then squeezes the liquid blocking cover 134 in the placement portion 130, and drives the liquid blocking cover 134 to move upward inside the fixed tube 132. At this time, the protein extract passes through the tube wall filter 133 and the liquid through hole 361 and then flows into the interior of the movable tank 350;

[0060] S6. As the liquid blocking cover 134 moves, the lifting rod 135 moves upward with it, driving the position of the placing plate 270 to move upward, and the plate surface slot 271 on the placing plate 270 immediately conflicts with the bottom surface of the inner convex ring for placing the limit block 250 in the centrifugal cylinder 210. As the centrifugal cylinder 210 continues to rotate, the limit block 250 is pushed into the inside of the plate surface slot 271 under the action of the downward pressure spring 260, driving the placing plate 270 to rotate as a whole;

[0061] S7, at this time, the output power of the driving motor 410 is increased, and the centrifugal cylinder 210 and the placing plate 270 are driven to rotate at a high speed, so that the protein extract retained in the centrifugal cylinder 210 is filtered by centrifugation and flows into the interior of the movable tank 350;

[0062] S8, after the electric push rod 370 is controlled to restore the position of the movable tank 350, as the driving motor 410 is started, the rotating cover 310 that is rotating will mix the protein extract and the neutral protease placed in the movable tank 350 in advance through the internal stirring rod 330, so that the protein in the extract is enzymatically hydrolyzed into amino acids and small peptides through the enzymatic reaction;

[0063] S9, after the enzymatic hydrolysis reaction is completed, the driving motor 410 is turned off, and the ball valve 340 is opened to discharge the enzymatic hydrolysis liquid from the interior of the movable tank 350;

[0064] S10. Subsequently, the enzymatic hydrolysate is subjected to ultrafiltration membrane separation technology to further purify the amino acids and small peptide products, and after removing macromolecular impurities and salts, the purified products are concentrated and dried to obtain amino acid biostimulant products prepared by fish protein hydrolysis;

[0065] When in use, the driving device 400 is used to drive the fish paste processing device 200 in the fixing device 100 and the structure in the enzymatic hydrolysis device 300 to rotate, thereby completing the enzymatic hydrolysis reaction of the marine fish protein, and further completing the production of the amino acid biostimulant;

[0066] In this embodiment, Figure 3 As shown, the fixing device 100 includes a protein extraction tank 110, a jacket tank body 120 disposed outside the bottom of the protein extraction tank 110, and a placement portion 130 located at the bottom surface of the protein extraction tank 110;

[0067] Specifically, a fixing ring body 111 is integrally formed on the inner wall of the protein extraction tank 110 near the top, the outer shell tank body 120 is welded and fixed to the outer side of the protein extraction tank 110, and a convex chamber body 121 is integrally formed on the outer wall at the left and right ends of the outer shell tank body 120.

[0068] Furthermore, a connecting box 140 is integrally formed on the front outer side walls of the protein extraction tank 110 and the outer shell tank body 120, a plurality of support frames 150 are welded and fixed on the outer side wall of the outer shell tank body 120, and a sealing cover 160 is rotatably connected to the outer side of the top opening of the protein extraction tank 110 through a hinge.

[0069] Furthermore, the protein extraction tank 110 is used to provide a placement area for the fish paste processing device 200, the fixed ring body 111 is used to limit the overall rotation area of ​​the fish paste processing device 200, the outer tank body 120 is used to provide a placement area for the enzymatic hydrolysis device 300, the outer convex warehouse body 121 is used to provide a placement area for the structure inside the enzymatic hydrolysis device 300, the connecting box body 140 is used to provide a placement area for the structure inside the driving device 400, the support frame 150 is used to ensure the overall stability of the protein extraction tank 110, and the sealing cover 160 is used to ensure the airtightness of the internal space of the protein extraction tank 110.

[0070] In this embodiment, Figure 4 As shown, the placement portion 130 includes a placement platform 131, a fixed tube 132 disposed at the center of the bottom surface of the placement platform 131, a liquid blocking cover 134 slidably connected to the inside of the fixed tube 132, an upper lifting rod 135 disposed at the center of the top surface inside the liquid blocking cover 134, and a jacket spring 136 sleeved on the outside of the upper lifting rod 135;

[0071] Specifically, a limiting groove 1310 is opened at the top surface of the placement table 131, the fixed tube 132 is integrally formed with the placement table 131 and is fixed on the inner bottom surface of the protein extraction tank 110 by clamping, the through groove opened on the outer tube wall of the fixed tube 132 is connected to the tube wall filter 133 by screws, the lifting rod 135 is welded and fixed to the inner top surface of the liquid blocking cover 134, and the upper and lower ends of the outer sleeve spring 136 are respectively welded and fixed to the bottom surface of the placement table 131 and the inner top surface of the liquid blocking cover 134.

[0072] Furthermore, the limiting groove 1310 on the placement table 131 is used to limit the position of the internal structure of the fish paste processing device 200, the tube wall filter 133 in the through groove on the fixed tube 132 is used to further filter the protein extract, and the liquid blocking cover 134 is used to ensure the airtightness of the bottom of the protein extraction tank 110. When the liquid blocking cover 134 moves upward, it will drive the lifting rod 135 to move upward together, and the outer sleeve spring 136 provides a downward force on the liquid blocking cover 134 through its own elastic force.

[0073] In this embodiment, Figure 5-Figure 8 As shown, the fish paste processing device 200 includes a centrifugal cylinder 210 with a plurality of openings on the outer wall, a cylinder wall filter 220 arranged inside the openings of the centrifugal cylinder 210, a plurality of mixing rods 230 regularly arranged on the inner wall of the centrifugal cylinder 210, a cylinder wall ring gear 240 sleeved on the outer wall of the centrifugal cylinder 210 near the top position, and a plurality of limit blocks 250 regularly arranged on the inner wall of the centrifugal cylinder 210 near the bottom position, a downward pressure spring 260 for providing downward pressure to the plurality of limit blocks 250, and a placing plate 270 arranged at the bottom opening of the centrifugal cylinder 210;

[0074] Specifically, the centrifugal cylinder 210 is rotatably connected to the top surface of the placement table 131 , the cylinder wall filter 220 is fixedly connected to the open inner wall of the centrifugal cylinder 210 by screws, and the mixing rod 230 is clamped and fixed to the inner wall of the centrifugal cylinder 210 .

[0075] Furthermore, the cylinder wall ring tooth 240 is fixedly connected to the outer wall of the cylinder wall ring tooth 240 by a bayonet pin, the bottom horizontal height of the cylinder wall ring tooth 240 is higher than the top horizontal height of the fixed ring body 111, and the limit block 250 is slidably connected to the groove on the bottom surface of the inner convex ring on the inner wall of the centrifugal cylinder body 210 near the bottom surface, and the upper and lower ends of the downward pressure spring 260 are respectively welded and fixed to the top surface of the groove wall of the groove on the bottom surface of the inner convex ring of the centrifugal cylinder body 210 and the bottom surface of the limit block 250.

[0076] Furthermore, the supporting plate 270 is slidably connected to the bottom opening of the centrifugal cylinder 210, and the bottom surface of the upper ring plate of the supporting plate 270 is in conflict with the top surface of the inner convex ring of the centrifugal cylinder 210. The top surface of the lower ring plate of the supporting plate 270 is provided with a plurality of plate surface grooves 271 which are the same in number and correspond in position to the limit blocks 250, and a plate bottom plug block 272 which is adapted to the size of the limit groove 1310 is integrally formed at the center position of the bottom surface of the supporting plate 270.

[0077] Furthermore, the centrifugal cylinder 210 is used to provide a placement area for the processed fish paste, the cylinder wall filter 220 is used to filter the fish paste, and the mixing rod 230 is used to fully mix the fish paste and the salt solution after the centrifugal cylinder 210 rotates. After the position of the supporting plate 270 is moved up, the plate surface groove 271 on the supporting plate 270 immediately conflicts with the bottom surface of the inner convex ring for placing the limit block 250 in the centrifugal cylinder 210. As the centrifugal cylinder 210 continues to rotate, the limit block 250 is ejected into the inside of the plate surface groove 271 under the action of the downward pressure spring 260, thereby driving the supporting plate 270 to rotate as a whole. At this time, the fish paste in the centrifugal cylinder 210 will rotate together, and the internal protein extract will be separated from it.

[0078] In this embodiment, Fig. 9 As shown, the enzymatic hydrolysis device 300 includes a rotating cover body 310, a cover wall ring gear 320 sleeved on the outer wall of the rotating cover body 310, a stirring rod 330 arranged on the inner top surface of the rotating cover body 310, a ball valve 340 arranged outside the bottom pipe opening of the rotating cover body 310, a movable tank body 350 sleeved on the inner side of the rotating cover body 310, an upper lifting pipe 360 ​​arranged at the center position of the top surface of the movable tank body 350, and an electric push rod 370 used to drive the movable tank body 350 to move up and down;

[0079] Specifically, the rotating cover body 310 is rotatably connected to the bottom opening of the outer shell tank body 120, the cover wall ring gear 320 is fixedly connected to the outer wall of the outer shell tank body 120 by a bayonet, the stirring rod 330 is clamped and fixed to the inner top surface of the rotating cover body 310, and the ball valve 340 is flange-connected to the pipe opening on the bottom surface of the rotating cover body 310.

[0080] Furthermore, the movable tank body 350 is rotatably connected to the interior of the rotating cover body 310, the upper lifting tube 360 ​​is integrally formed with the movable tank body 350, and a plurality of regularly distributed liquid holes 361 that pass through the inside and outside are opened on the outer tube wall of the upper lifting tube 360. The electric push rod 370 is fixedly connected to the inner bottom surface of the outer convex warehouse body 121 by screws, and the top end of the telescopic rod of the electric push rod 370 is fixedly connected to the connecting plate 380 that is clamped and fixed to the outer wall of the movable tank body 350 by bolts.

[0081] Furthermore, the electric push rod 370 in the enzymolysis device 300 is started, and the movable tank body 350 is driven to move upward as a whole through the connecting plate 380, and the lifting tube 360 ​​is driven to move upward together. The lifting tube 360 ​​then squeezes the liquid blocking cover 134 in the placement portion 130, and drives the liquid blocking cover 134 to move upward inside the fixed tube 132. At this time, the protein extract passes through the tube wall filter 133 and the liquid hole 361 and is poured into the movable tank body 350. Then, the electric push rod 370 is controlled to restore the position of the movable tank body 350. As the driving motor 410 is started, the rotating cover body 310 that rotates will mix the protein extract and the neutral protease placed in the movable tank body 350 in advance through the internal stirring rod 330, so that the protein in the extract is enzymatically hydrolyzed into amino acids and small peptides through the enzymatic reaction. The enzymolysis solution can be discharged by subsequently opening the ball valve 340.

[0082] In this embodiment, Fig.10 As shown, the driving device 400 includes a driving motor 410, a driving gear 420 sleeved on the outside of the output shaft of the driving motor 410, and a docking gear 430 that rotates as the driving gear 420 rotates;

[0083] Specifically, the drive motor 410 is fixedly connected to the top surface of the connecting box 140 by bolts, the drive gear 420 is fixedly connected to the output shaft of the drive motor 410 by a bayonet pin, the drive gear 420 is meshed with the cylinder wall ring gear 240, the docking gear 430 is rotatably connected to the inner bottom surface of the connecting box 140 and meshed with the cover wall ring gear 320, and the outer sides of the drive gear 420 and the docking gear 430 are provided with a rotating shaft 440 whose upper and lower ends are rotatably connected to the inside of the connecting box 140, and the upper and lower ends of the rotating shaft 440 are both clamped and fixed with shaft end gears 450 that are respectively meshed with the drive gear 420 and the docking gear 430.

[0084] Furthermore, after the driving motor 410 is started, it drives the driving gear 420 to rotate. After the driving gear 420 contacts the shaft end gear 450 at the top of the rotating shaft 440, it drives the rotating shaft 440 to rotate, and then drives the shaft end gear 450 located below to rotate, thereby driving the docking gear 430 to rotate.

[0085] The foregoing description of specific exemplary embodiments of the present invention is for the purpose of illustration and demonstration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is clear that many changes and variations can be made based on the above teachings. The purpose of selecting and describing exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can realize and utilize various different exemplary embodiments of the present invention and various different selections and changes. The scope of the present invention is intended to be limited by the specification and its equivalents.

Claims

1. A production device for preparing amino acid biostimulants by enzymatic hydrolysis of marine fish protein, wherein when in use, a driving device (400) is used to drive the fish paste processing device (200) in the fixing device (100) and the structure in the enzymatic hydrolysis device (300) to rotate, thereby completing the enzymatic hydrolysis reaction of the marine fish protein, and further completing the production of the amino acid biostimulant; Features: The fixing device (100) comprises a protein extraction tank (110), an outer tank body (120) arranged outside the bottom of the protein extraction tank (110), and a placement portion (130) located at the bottom surface of the protein extraction tank (110); The placement portion (130) comprises a placement platform (131), a fixed tube (132) disposed at the center of the bottom surface of the placement platform (131), a liquid blocking cover (134) slidably connected to the inside of the fixed tube (132), an upper lifting rod (135) disposed at the center of the top surface inside the liquid blocking cover (134), and a jacket spring (136) sleeved on the outside of the upper lifting rod (135); The fish paste processing device (200) comprises a centrifugal cylinder (210) having a plurality of openings on its outer wall, a cylinder wall filter (220) arranged inside the openings of the centrifugal cylinder (210), a plurality of mixing rods (230) regularly arranged on the inner wall of the centrifugal cylinder (210), a cylinder wall ring tooth (240) sleeved on the outer wall of the centrifugal cylinder (210) near the top, a plurality of limit blocks (250) regularly arranged on the inner wall of the centrifugal cylinder (210) near the bottom, a downward pressure spring (260) for providing downward pressure to the plurality of limit blocks (250), and a receiving plate (270) arranged at the bottom opening of the centrifugal cylinder (210); The enzymatic hydrolysis device (300) comprises a rotating cover body (310), a cover wall ring gear (320) sleeved on the outer wall of the rotating cover body (310), a stirring rod (330) arranged on the inner top surface of the rotating cover body (310), a ball valve (340) arranged on the outer side of the bottom pipe opening of the rotating cover body (310), a movable tank body (350) sleeved on the inner side of the rotating cover body (310), an upward lifting pipe (360) arranged at the center position of the top surface of the movable tank body (350), and an electric push rod (370) used to drive the movable tank body (350) to move up and down; The driving device (400) comprises a driving motor (410), a driving gear (420) sleeved on the outside of an output shaft of the driving motor (410), and a docking gear (430) that rotates as the driving gear (420) rotates; A fixing ring body (111) is integrally formed at a position near the top of the inner wall of the protein extraction tank (110); the outer shell tank body (120) is welded and fixed to the outer side of the protein extraction tank (110); and outer convex chamber bodies (121) are integrally formed on the outer walls at the left and right ends of the outer shell tank body (120); A connecting box (140) is integrally formed on the front outer side walls of the protein extraction tank (110) and the outer shell tank body (120); a plurality of support frames (150) are welded and fixed to the outer side wall of the outer shell tank body (120); and a sealing cover (160) is rotatably connected to the outer side of the top opening of the protein extraction tank (110) via a hinge; A limiting groove (1310) is provided at the top surface of the placement platform (131); the fixed tube (132) is integrally formed with the placement platform (131) and is fixedly connected to the inner bottom surface of the protein extraction tank (110); the inner parts of the through grooves provided on the outer tube wall of the fixed tube (132) are fixedly connected to the tube wall filter (133) by screws; the lifting rod (135) is welded and fixed to the inner top surface of the liquid blocking cover (134); and the upper and lower ends of the outer sleeve spring (136) are respectively welded and fixed to the bottom surface of the placement platform (131) and the inner top surface of the liquid blocking cover (134); The centrifugal cylinder (210) is rotatably connected to the top surface of the placement platform (131), the cylinder wall filter (220) is fixedly connected to the open inner wall of the centrifugal cylinder (210) by screws, and the mixing rod (230) is clamped and fixed to the inner wall of the centrifugal cylinder (210); The cylinder wall ring tooth (240) is fixedly connected to the outer wall of the cylinder wall ring tooth (240) by means of a bayonet pin, the bottom level of the cylinder wall ring tooth (240) is higher than the top level of the fixed ring body (111), the limit block (250) is slidably connected to the groove on the bottom surface of the inner convex ring near the bottom surface of the inner wall of the centrifugal cylinder body (210), and the upper and lower ends of the downward pressure spring (260) are respectively welded and fixed to the top surface of the groove wall of the groove on the bottom surface of the inner convex ring of the centrifugal cylinder body (210) and the bottom surface of the limit block (250); The placing plate (270) is slidably connected to the bottom opening of the centrifugal cylinder (210); the bottom surface of the placing plate (270) located on the upper ring plate abuts against the top surface of the inner convex ring of the centrifugal cylinder (210); the top surface of the placing plate (270) located on the lower ring plate is provided with a plurality of plate surface slots (271) the same in number as the limiting blocks (250) and corresponding in position; a plate bottom plug block (272) matching the size of the limiting slot (1310) is integrally formed at the center of the bottom surface of the placing plate (270); The rotating cover (310) is rotatably connected to the bottom opening of the outer tank body (120); the cover wall ring gear (320) is fixedly connected to the outer wall of the outer tank body (120) via a bayonet; the stirring rod (330) is fixedly connected to the inner top surface of the rotating cover (310); the ball valve (340) is flange-connected to the pipe opening of the bottom surface of the rotating cover (310); the movable tank body (350) is rotatably connected to the rotating cover (310); 0), the upper lifting tube (360) and the movable tank body (350) are integrally formed, a plurality of regularly distributed liquid-through holes (361) that are connected inside and outside are opened on the outer tube wall of the upper lifting tube (360), the electric push rod (370) is fixedly connected to the inner bottom surface of the outer convex tank body (121) by screws, and the top end of the telescopic rod of the electric push rod (370) is fixedly connected to a connecting plate (380) that is clamped and fixed to the outer wall of the movable tank body (350) by bolts.

2. The production device for preparing amino acid biostimulants by enzymatic hydrolysis of marine fish protein according to claim 1, characterized in that: The driving motor (410) is fixedly connected to the top surface of the connecting box (140) by means of bolts, the driving gear (420) is fixedly connected to the output shaft of the driving motor (410) by means of a bayonet pin, the driving gear (420) is meshed with the cylinder wall ring gear (240), the docking gear (430) is rotatably connected to the inner bottom surface of the connecting box (140) and meshed with the cover wall ring gear (320), the outer sides of the driving gear (420) and the docking gear (430) are provided with a rotating shaft (440) whose upper and lower ends are rotatably connected to the inside of the connecting box (140), and the upper and lower ends of the rotating shaft (440) are both clamped and fixed with shaft end gears (450) respectively meshed with the driving gear (420) and the docking gear (430).

3. The production device for preparing amino acid biostimulants by enzymatic hydrolysis of marine fish protein according to claim 2, characterized in that: The following steps are involved in use: S1. First, marine fish are selected as raw materials, cleaned, scaled, and eviscerated, and then mechanically crushed into fish paste; S2, then, opening the sealing cover (160) in the fixing device (100), placing the processed fish paste into the interior of the centrifugal cylinder (210) in the fish paste processing device (200), and pouring a salt solution into the protein extraction tank (110); S3, after the pH value of the salt solution is adjusted to acidic by using dilute hydrochloric acid, the sealing cover (160) is closed again, and the driving motor (410) in the driving device (400) is started to drive the driving gear (420) to rotate, and after the driving gear (420) contacts the cylinder wall ring gear (240), the centrifugal cylinder (210) is driven to rotate as a whole, and the mixing rod (230) fully stirs and mixes the fish paste and the salt solution, and the protein in the fish paste is dissolved in the salt solution; S4. Subsequently, the electric push rod (370) in the enzymolysis device (300) is started to drive the movable tank (350) to move upward as a whole through the connecting plate (380), thereby driving the lifting tube (360) to move upward together; S5, the lifting tube (360) then squeezes the liquid blocking cover (134) in the placement portion (130), thereby driving the liquid blocking cover (134) to move upward inside the fixed tube (132). At this time, the protein extract passes through the tube wall filter (133) and the liquid through hole (361) and then flows into the interior of the movable tank (350); S6. As the liquid blocking cover (134) moves, the lifting rod (135) moves upward with it, driving the position of the placing plate (270) to move upward, and the plate surface slot (271) on the placing plate (270) then abuts against the bottom surface of the inner convex ring for placing the limit block (250) in the centrifugal cylinder (210). As the centrifugal cylinder (210) continues to rotate, the limit block (250) is pushed into the inside of the plate surface slot (271) under the action of the downward pressure spring (260), driving the placing plate (270) to rotate as a whole; S7, at this time, the output power of the driving motor (410) is increased, thereby driving the centrifugal cylinder (210) and the placing plate (270) to rotate at a high speed, so that the protein extract retained in the centrifugal cylinder (210) is filtered by centrifugation and flows into the interior of the mobile tank (350); S8, after the electric push rod (370) is controlled to restore the position of the movable tank (350), the rotating cover (310) is rotated as the driving motor (410) is started, and the protein extract and the neutral protease placed in the movable tank (350) in advance are mixed through the internal stirring rod (330), so that the protein in the extract is enzymatically hydrolyzed into amino acids and small peptides through an enzymatic reaction; S9, after the enzymatic hydrolysis reaction is completed, the driving motor (410) is turned off, and the ball valve (340) is opened to discharge the enzymatic hydrolysis liquid from the interior of the movable tank (350); S10. Subsequently, the enzymatic hydrolysate is subjected to ultrafiltration membrane separation technology to further purify the amino acids and small peptide products, and after removing the macromolecular impurities and salts, the purified products are concentrated and dried to obtain the amino acid biostimulant product prepared by fish protein hydrolysis.

Citation Information

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